CN103468619B - A kind of microorganism formulation purifying air - Google Patents
A kind of microorganism formulation purifying air Download PDFInfo
- Publication number
- CN103468619B CN103468619B CN201310443794.4A CN201310443794A CN103468619B CN 103468619 B CN103468619 B CN 103468619B CN 201310443794 A CN201310443794 A CN 201310443794A CN 103468619 B CN103468619 B CN 103468619B
- Authority
- CN
- China
- Prior art keywords
- parts
- pseudomonas putida
- streptococcus thermophilus
- microbial agent
- enterococcus faecium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The present invention provides a kind of microorganism formulations for purifying air, pass through the synergistic effect between streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, the concentration of the foul smell such as the ammonia in the air of the places such as lavatory, farm, soot and hydrogen sulfide can be effectively reduced, inhibit to produce smelly element, to significantly improve air quality and people's lives environment.Production method provided by the invention is simple, is adapted to most of existing zymotechniques and equipment, is conducive to large-scale promotion application.
Description
Technical field
The present invention relates to technical field of microbe application, and in particular to it is a kind of purify air microorganism formulation and its answer
With.
Background technique
Odor pollution is universally acknowledged one of seven overall situation public hazards.Common odorant pollutant has ammonia, trimethylamine, vulcanization
Hydrogen, methyl mercaptan, methyl disulfide, carbon disulfide and styrene etc. are mainly derived from industrial and agricultural production department and people's lives, example
The excrement generated in such as agriculture-stock production and processing is smelly, fish is smelly, corruption is smelly, decayed fruit is smelly, edible wild herbs are smelly.In petrochemical production process
Sulfide, hydro carbons, benzene class, phenols and the Ammonia and tar of generation, pitch steam, ammonia and various organic solvents and city
The stench of communal facility.The odorant pollutant of high concentration has direct harm to human health.
Currently, can be divided into two broad aspect of chemical deodorizing and biological deodorizing to stench removal methods, chemical deodorizing principle is logical
It crosses external source Strong oxdiative substance and oxidative degradation is carried out to stench composition, closely eliminate stench, though it is with strong points, it is quick, its
Application range is small, and when encountering complex effluvium gas ingredients, deodorization efficiency will fail, and use remainingization after chemical deodorizing agent
It learns substance and often causes secondary pollution.Biological deodorizing principle is the direct drop by external source function strain or biological enzyme to stench
It solves or stench is removed to the inhibition for producing stench microorganism.Biological deodorizing can persistently eliminate stench, without secondary pollution, user
Just, the features such as low in cost, becomes the research hotspot of stench control, finds biological deodorizing product effectively, green and method, at
For the urgent need of present society.
Summary of the invention
The present invention is to solve prior art problem, and odorant pollutant in air can be removed by providing one kind, and then be purified
The complex microorganism preparations of air.Said preparation passes through the collaboration facilitation of multiple-microorganism, can effectively inhibit to produce smelly element, drops
Odorant pollutant is solved, to reduce the concentration of pernicious gas, reduces odor concentration.
Applicant had found by long-term screening study, streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris,
Synergistic effect can be generated between bacillus pumilus and enterococcus faecium, and the foul smell such as ammonia and hydrogen sulfide in air are effectively reduced
Concentration, to facilitate the present invention.
One aspect of the present invention provides a kind of deodorant microbial agent, by streptococcus thermophilus (Streptococcus thermophilus), pseudomonas putida (pseudomonas putida), Rhodopseudomonas palustris
(Rhodopseudomonas palustris), bacillus pumilus (Bacillus pumilus) and enterococcus faecium
(Enterococcus faecium) composition.
The preferred streptococcus thermophilus CGMCC1.3996 of each component, pseudomonas putida in above-mentioned deodorant microbial agent
CGMCC1.2309, Rhodopseudomonas palustris CGMCC1.2349, bacillus pumilus CGMCC1.8112 and enterococcus faecium
CGMCC1.2136。
Each component and its content are respectively as follows: 40-60 parts of streptococcus thermophilus, Pseudomonas putida in above-mentioned deodorant microbial agent
35-40 parts of bacterium, 18-30 parts of Rhodopseudomonas palustris, 25-50 parts of bacillus pumilus and 45-80 parts of enterococcus faecium.
The content of each component is respectively preferably in above-mentioned deodorant microbial agent: 45 parts of streptococcus thermophilus, Pseudomonas putida
38 parts of bacterium, 25 parts of Rhodopseudomonas palustris, 40 parts of bacillus pumilus and 55 parts of enterococcus faecium.
Another aspect of the present invention provides the preparation method of above-mentioned deodorant microbial agent, includes the following steps:
1) by streptococcus thermophilus CGMCC1.3996, pseudomonas putida CGMCC1.2309, Rhodopseudomonas palustris
High density liquid hair is respectively adopted in CGMCC1.2349, bacillus pumilus CGMCC1.8112 and enterococcus faecium CGMCC1.2136
Ferment after fermentation liquid is lyophilized, is made spore content and is up to 1010-1011The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 40-60 parts of streptococcus thermophilus, 35-40 parts of pseudomonas putida, the red false unit cell in marsh
18-30 parts of bacterium, 25-50 parts of bacillus pumilus and 45-80 parts of enterococcus faecium are configured to complex microorganism preparations, and as solid removes
Smelly microorganism formulation；
3) microorganism formulation made from step 2 is taken, the sodium citrate for being 8-10% by 1-5% mass volume ratio addition concentration
Solution is uniformly mixed to get liquid deodorant microorganism formulation of the invention.
Another aspect of the present invention provides application of the above-mentioned deodorant microbial agent in purification air.
Aforesaid liquid deodorant microbial agent is sprayed on the highly concentrated place of the malodorants such as lavatory, farm, soot,
It sprays 2-3 times weekly, each dosage 100-200 mL/ m3。
Beneficial effect
Complex microorganism preparations provided by the invention by streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris,
Synergistic effect between bacillus pumilus and enterococcus faecium can be effectively reduced in the air of the places such as lavatory, farm, soot
Ammonia and the foul smell such as hydrogen sulfide concentration, inhibit to produce smelly element, to significantly improve air quality and people's lives environment.
Production method provided by the invention is simple, is adapted to most of existing zymotechniques and equipment, is conducive to large-scale promotion application.
Specific embodiment
Various reagents and equipment of the present invention, can be selected from it is commercially available any one；The present invention relates to thermophilic in bacterial strain
Streptococcus is purchased from China General Microbiological preservation administrative center, deposit number CGMCC1.3996；Pseudomonas putida is purchased from China
Common micro-organisms preservation administrative center, deposit number CGMCC1.2309；Rhodopseudomonas palustris is that the azure biological group in Qingdao has
Limit house journal's bacterial strain, deposit number CGMCC1.2349；Bacillus pumilus is purchased from China General Microbiological preservation management
The heart, deposit number CGMCC1.8112；Enterococcus faecium is purchased from China General Microbiological preservation administrative center, deposit number
CGMCC1.2136。
Present invention will be further explained below with reference to specific examples.
Embodiment 1
A kind of deodorant microbial agent, by streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, short and small gemma
Bacillus and enterococcus faecium composition, each component and its content are respectively as follows: 40 parts of streptococcus thermophilus, 40 parts of pseudomonas putida, marsh
30 parts of red pseudomonas, 25 parts of bacillus pumilus and 45 parts of enterococcus faecium.
The preparation method of above-mentioned deodorant microbial agent, includes the following steps:
1) streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium are distinguished
It is fermented using high density liquid, after fermentation liquid is lyophilized, spore content is made and is up to 1010-1011The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 40 parts of streptococcus thermophilus, 40 parts of pseudomonas putida, 30 parts of Rhodopseudomonas palustris,
25 parts and 45 parts of enterococcus faecium of bacillus pumilus are configured to complex microorganism preparations, as solid deodorant microbial agent；
3) microorganism formulation made from step 2 is taken, the sodium citrate solution for being 10% by 1% mass volume ratio addition concentration,
It is uniformly mixed to get liquid deodorant microorganism formulation of the invention.
Embodiment 2
A kind of deodorant microbial agent, by streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, short and small gemma
Bacillus and enterococcus faecium composition, each component and its content are respectively as follows: 60 parts of streptococcus thermophilus, 35 parts of pseudomonas putida, marsh
18 parts of red pseudomonas, 50 parts of bacillus pumilus and 80 parts of enterococcus faecium.
The preparation method of above-mentioned deodorant microbial agent, includes the following steps:
1) streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium are distinguished
It is fermented using high density liquid, after fermentation liquid is lyophilized, spore content is made and is up to 1010-1011The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 60 parts of streptococcus thermophilus, 35 parts of pseudomonas putida, 18 parts of Rhodopseudomonas palustris,
50 parts and 80 parts of enterococcus faecium of bacillus pumilus are configured to complex microorganism preparations, as solid deodorant microbial agent；
3) microorganism formulation made from step 2 is taken, the sodium citrate solution for being 8% by 5% mass volume ratio addition concentration,
It is uniformly mixed to get liquid deodorant microorganism formulation of the invention.
Embodiment 3
A kind of deodorant microbial agent, by streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, short and small gemma
Bacillus and enterococcus faecium composition, each component and its content are respectively as follows: 45 parts of streptococcus thermophilus, 38 parts of pseudomonas putida, marsh
25 parts of red pseudomonas, 40 parts of bacillus pumilus and 55 parts of enterococcus faecium.
The preparation method of above-mentioned deodorant microbial agent, includes the following steps:
1) streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium are distinguished
It is fermented using high density liquid, after fermentation liquid is lyophilized, spore content is made and is up to 1010-1011The hyperconcetration bacterium powder of CFU/g；
2) by hyperconcetration bacterium powder according to 45 parts of streptococcus thermophilus, 38 parts of pseudomonas putida, 25 parts of Rhodopseudomonas palustris,
40 parts and 55 parts of enterococcus faecium of bacillus pumilus are configured to complex microorganism preparations, as solid deodorant microbial agent；
3) microorganism formulation made from step 2 is taken, the sodium citrate solution for being 9% by 4% mass volume ratio addition concentration,
It is uniformly mixed to get liquid deodorant microorganism formulation of the invention.
The application of 4 deodorant microbial agent of embodiment
1,100g solid-state deodorizing microorganism system of the invention is added into the beaker (2L) equipped with the fresh pig manure of 1000g
After agent and excrement mix, preservative film sealed beaker；While the control of microbial inoculum is not added, and three repetitions of every group of experimental setup, 7 days
It is as shown in table 1 below that stench intensity, ammonia concentration and concentration of hydrogen sulfide, measurement result are measured afterwards:
| Testing index | Control group | Add microorganism formulation group | Reduced rate |
| Odor strength | 297312 | 178387 | 40.0% |
| Ammonia concentration | 42.3 | 21.4 | 49.4% |
| Concentration of hydrogen sulfide | 156.1 | 77.2 | 50.5% |
As it can be seen from table 1 after addition deodorant microbial agent of the invention, ammonia that excrement generates and hydrogen sulfide it is dense
Degree reduces 49.4% and 50.5% respectively, significantly reduces odor strength, illustrates that microorganism formulation of the invention has and removes well
Smelly effect can effectively improve air quality.
2, it chooses a house refuse by the river Li Cun to leave concentratedly as test site, liquid of the invention is sprayed in detection respectively
Before body deodorant microbial agent and sprinkling (sprays dosage 150g/ m3) afterwards the stench intensity in the 3rd day air, ammonia concentration and
Concentration of hydrogen sulfide, measurement result are as shown in table 2 below:
| Testing index | Before sprinkling | After sprinkling | Reduced rate |
| Odor strength | 312410 | 164371 | 47.4% |
| Ammonia concentration | 56.1 | 27.8 | 50.4% |
| Concentration of hydrogen sulfide | 152.8 | 79.1 | 48.2% |
From table 2 it can be seen that odor strength significantly reduces in soot air after spraying deodorizing microorganism of the invention
47.4%, wherein ammonia concentration reduces 50.4%, and concentration of hydrogen sulfide reduces 48.2%, illustrates microorganism formulation of the invention
Deodorizing effect is significant, can effectively reduce the concentration of odorant in air, be conducive to better people's living environment.
Claims (5)
1. a kind of deodorant microbial agent, by streptococcus thermophilus (Streptococcus thermophilus) CGMCC No.
1.3996, pseudomonas putida (pseudomonas putida ) CGMCC No. 1.2309, Rhodopseudomonas palustris (Rhodopseudomonas palustris ) CGMCC No. 1.2349, bacillus pumilus (Bacillus pumilus )
CGMCC No. 1.8112 and enterococcus faecium (Enterococcus faecium ) composition of CGMCC No. 1.2136.
2. deodorant microbial agent as described in claim 1, which is characterized in that the content of each component is respectively as follows: streptococcus thermophilus
40-60 parts, 35-40 parts of pseudomonas putida, 18-30 parts of Rhodopseudomonas palustris, 25-50 parts of bacillus pumilus and dung intestines ball
45-80 parts of bacterium.
3. deodorant microbial agent as claimed in claim 2, which is characterized in that the content of each component is respectively as follows: streptococcus thermophilus
45 parts, 38 parts of pseudomonas putida, 25 parts of Rhodopseudomonas palustris, 40 parts of bacillus pumilus and 55 parts of enterococcus faecium.
4. application of the deodorant microbial agent described in claim 1 in purification air.
5. the application of deodorant microbial agent as claimed in claim 4, which is characterized in that the sprinkling dosage of deodorant microbial agent
For 100-200g/m3, use 2-3 times weekly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310443794.4A CN103468619B (en) | 2013-09-26 | 2013-09-26 | A kind of microorganism formulation purifying air |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310443794.4A CN103468619B (en) | 2013-09-26 | 2013-09-26 | A kind of microorganism formulation purifying air |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103468619A CN103468619A (en) | 2013-12-25 |
| CN103468619B true CN103468619B (en) | 2019-03-26 |
Family
ID=49793662
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310443794.4A Active CN103468619B (en) | 2013-09-26 | 2013-09-26 | A kind of microorganism formulation purifying air |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103468619B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104946544A (en) * | 2015-06-30 | 2015-09-30 | 防城港市盛吉科技有限公司 | Microbial deodorizing inoculant |
| CN106036320A (en) * | 2016-06-16 | 2016-10-26 | 辽宁省微生物科学研究院 | Method for preparing mushroom mycelial fermentation broth original drink |
| CN106967640A (en) * | 2017-04-11 | 2017-07-21 | 上海莱泰生物科技有限公司 | A kind of preparation method of deodorizing microorganism microbial inoculum |
| CN111548960A (en) * | 2020-05-13 | 2020-08-18 | 深圳市东荣生物科技有限责任公司 | Compound microbial preparation for deodorization |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
| CN111893071A (en) * | 2020-08-12 | 2020-11-06 | 浙江丹靓环境工程有限公司 | Soil deodorization microbial preparation and application thereof |
| CN112608859B (en) * | 2020-12-17 | 2022-06-07 | 新疆河润水业有限责任公司 | Deodorant based on microorganisms and preparation method and application thereof |
| CN114891662B (en) * | 2022-03-05 | 2023-11-24 | 闽江学院 | Pseudomonas putida and application thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07328104A (en) * | 1994-06-10 | 1995-12-19 | Konan Kagaku Kogyo Kk | Deodorant |
| CN101671643B (en) * | 2009-09-27 | 2011-09-28 | 上海创博生态工程有限公司 | Method for preparing deodorizer by composite microorganism |
| CN101845394A (en) * | 2010-04-16 | 2010-09-29 | 安徽省农业科学院畜牧兽医研究所 | Fecal deodorizing microorganism combining bacteria and method for producing organic fertilizer using same |
| CN102258796A (en) * | 2011-06-28 | 2011-11-30 | 黄小娃 | Microbial deodorant and preparation method thereof |
| KR101263366B1 (en) * | 2012-05-01 | 2013-05-21 | 정천희 | The clealhouse with removal of bad smell and new renewable energy |
| CN102851210B (en) * | 2012-06-11 | 2013-09-04 | 中国科学院成都生物研究所 | Livestock and poultry excrement microbial deodorizing agent, and preparation method and application thereof |
-
2013
- 2013-09-26 CN CN201310443794.4A patent/CN103468619B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103468619A (en) | 2013-12-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103468619B (en) | A kind of microorganism formulation purifying air | |
| CN103463654B (en) | A method of producing deodorant microbial agent | |
| KR101260872B1 (en) | Composition for removing bad stock raising comprising microorganism probiotics as effective ingredient | |
| CN102247611B (en) | Microorganism deodorant and preparation method thereof | |
| KR102177670B1 (en) | Composition for removing odor and, manufacturing method thereof | |
| CN107189972A (en) | Microbial deodorant, preparation method and the usage | |
| CN105536509A (en) | Plant extract and microbe composite deodorant | |
| Matusiak et al. | The use of Yucca schidigera and microbial preparation for poultry manure deodorization and hygienization | |
| CN101711885A (en) | Method for preparing high-concentration biological deodorant | |
| CN106282038A (en) | A kind of composite efficient microbial deodorant and preparation method thereof | |
| CN104611258A (en) | Bacillus subtilis and biological deodorant containing bacterial strain of bacillus subtilis | |
| CN104307012A (en) | Deodorant special for livestock and poultry farms and application thereof | |
| CN104946544A (en) | Microbial deodorizing inoculant | |
| CN104258440A (en) | Biological deodorant and preparation method thereof | |
| CN104722200A (en) | Plant enzyme air purifying preparation | |
| CN104096249A (en) | Method for preparing deodorant using autotrophs and deodorant | |
| CN105435279B (en) | A kind of compound deodorizer coordinates the deodorizing method of washing deodorizing tower | |
| CN102872475A (en) | Biological agent for deodorizing and reducing chromium and preparation method thereof | |
| CN109609394A (en) | A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof | |
| CN114015586A (en) | Deodorizing and degrading composite microbial inoculum as well as preparation method and application thereof | |
| CN106967643A (en) | A kind of Novel compound microbial agent and preparation method thereof | |
| CN105642105A (en) | Intelligent biological deodorizer | |
| CN109762767A (en) | A kind of sewage complex microorganism inorganic agent and the preparation method and application thereof | |
| CN103275909B (en) | Phthalate degrading bacteria and application thereof | |
| CN103173384B (en) | Lactobacillus strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221125 Address after: 273400 west side of Yan Bin Road, Feixian County Economic Development Zone, Linyi, Shandong Patentee after: SHANDONG KDN BIOTECH Co.,Ltd. Patentee after: QINGDAO VLAND BIOTECH GROUP Co.,Ltd. Address before: 266061 room 12A07, Shandong Expressway Building, 29 Miaoling Road, Laoshan District, Qingdao City, Shandong Province Patentee before: QINGDAO VLAND BIOTECH GROUP Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230328 Address after: No. 596-1, jiushui East Road, Laoshan District, Qingdao City, Shandong Province 266100 Patentee after: QINGDAO VLAND BIOTECH GROUP Co.,Ltd. Address before: 273400 west side of Yan Bin Road, Feixian County Economic Development Zone, Linyi, Shandong Patentee before: SHANDONG KDN BIOTECH Co.,Ltd. Patentee before: QINGDAO VLAND BIOTECH GROUP Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230725 Address after: Room 304, no.6, Lane 88, Shengrong Road, Pudong New Area pilot Free Trade Zone, Shanghai 201203 Patentee after: SHANGHAI KDN BIOTECH Co.,Ltd. Address before: No. 596-1, jiushui East Road, Laoshan District, Qingdao City, Shandong Province 266100 Patentee before: QINGDAO VLAND BIOTECH GROUP Co.,Ltd. |
|
| TR01 | Transfer of patent right |