JPH07328104A - Deodorant - Google Patents

Abstract

PURPOSE:To obtain excellent deodorizing action by preparing a deodorant to deodorize a nasty smell of an organic sludge generated from sewage, urine or the like from a culture solution containing a metabolite of a microorganism comprising fungi of pseudomonas putida and pseudomonas fluorescence in the genus of pseudomonas. CONSTITUTION:This deodorant is prepared from a culture solution containing a metabolite of a microorganism produced by culturing at least one microorganism as selected from the fungi of pseudomonas putida and pseudomonas fluorescence in the genus of pseudomonas. A culture medium herein used is the one for culturing aerobic gram negative bacteria, preferably a composition of polypeptone, yeast extraction, sodium chloride, hydrogen potassium phosphate and a degerming water. The culturing conditions are the introduction of air or oxygen under an agitation, the liquid temperature in the range of 20 deg.C-30 deg.C and the duration for 10-200 hours. This reduces the generation of any malodorous substance and eliminates adverse effect on the post process and possibility of secondary pollution.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a deodorant used for suppressing odors generated from organic sludge in wastewater treatment processes such as various industrial wastewater.

[0002]

2. Description of the Related Art (1) Masking agents using aromatic substances (sensory action) (2) Oxidizing agents and reducing agents (sodium hypochlorite) , Sodium sulfite, ozone, etc.) (3) Acidic agents, alkaline agents (4) Vegetable oils extracted from natural products (5) Methacrylic acid esters, maleic acid esters, etc. (deodorization by addition reaction) (6) Glyoxal There are aldehydes (deodorization due to condensation reaction).

[0003]

DISCLOSURE OF THE INVENTION At present, the above-mentioned deodorant is effective only in a closed place such as a refrigerator or a car, or is effective only in a very weak odor, and is economically unfavorable. The current situation is that it is stable, dangerous to handle, remains on the object to be treated, adversely affects the post-process, requires new countermeasures for secondary pollution, and is insufficient.

It is desirable that the deodorant has a strong odor such as sludge, is still safe while preventing the odor generated in the open state, does not affect the subsequent processes, and is free from the concern of secondary pollution. . Nothing that can meet such a demand has been proposed yet.

Under the above circumstances, the present invention provides sewage,
The invention was devised for the purpose of providing a deodorant that highly efficiently deodorizes the offensive odor of organic sludge generated from the treatment of human waste or general industrial wastewater.

[0006]

[MEANS FOR SOLVING THE PROBLEMS] At least one kind of microorganism selected from Pseudomonas putida and Pseudomonas fluorescens belonging to the genus Pseudomonas is cultivated to form a culture solution containing a microbial metabolite.

Specific microorganisms include the following. Pseudomonas Putida (NRRL-B-12538) Pseudomonas Putida (NRRL-B-12539) Pseudomonas Fluorescence (NRRL-B-1)
8296) As a medium for culturing this microorganism, a general medium for culturing aerobic Gram-negative bacteria can be used, but one having the following ratio is preferable. Polypeptone 0.1 to 10 g Yeast extract 0.1 to 5 g Sodium chloride 0 to 2.5 g Potassium hydrogen phosphate 0 to 2.5 g Sterilized water 50 to 225 g As culture conditions, air or oxygen is introduced under stirring, and liquid What was cultivated at a temperature in the range of 5 ° C to 35 ° C, preferably in the range of 20 ° C to 30 ° C for about 10 hours to 200 hours is preferable.

[0008]

By adding the microorganism culture solution of the present invention to odorous sludge, an excellent deodorizing effect is exhibited. These effects need to be elucidated in the future, but it is thought that the malodorous components are deodorized by oxidative decomposition and neutralization due to the functions of enzymes in microbial metabolites and the functions of other metabolites. To be

[0009]

EXAMPLES The present invention will be described more specifically with reference to the following examples.

(Example 1) Munox 112 * 6 g Polypeptone 12 g Yeast extract 6 g Sodium chloride 3 g Potassium hydrogen phosphate 3 g Distilled water 270 g * US-Sprey company trade name (Pseudomonas)
Putida and Pseudomonas Fluo
Rescens) The above composition, which has been sterilized in advance, has an internal volume of 500 m.
The mixture was placed in a glass culture bottle (mini furnter) equipped with a stirrer (1), sterilized air was introduced, and the mixture was cultured at 29 ° C. for 4 days with stirring.

The culture solution was centrifuged, and the separated solution was further filtered through a membrane filter to remove the bacterial cells to obtain a culture solution A.

Sludge from the underwater treatment plant (TS 1.70%, S
S15200PPM, pH 5.35, alkalinity 27
0, organic matter 80.4%, inorganic matter 19.6%) 10 each
0 g each was collected in a 300 ml Erlenmeyer flask, and the culture solution A of the present invention was added and stirred at each addition amount shown in Table 1 and then sealed and kept at 40 ° C. to measure the odorous component concentrations after 4 hours and 24 hours. did.

[0013]

[Table 1]

(Example 2) Munox 112 12 g Polypeptone 12 g Yeast extract 6 g Meat extract 1 g Sodium chloride 3 g Potassium hydrogen phosphate 3 g Distilled water 263 g The above composition sterilized in advance was charged into a glass culture bottle equipped with a stirrer and sterilized. The cells were cultured at 25 ° C. for 5 days with stirring while introducing air. Centrifuge this culture,
The separated liquid was further filtered with a membrane filter to obtain a culture liquid B from which bacterial cells were removed.

Sludge from Otsu sewerage treatment plant (TS 2.32%, S
S20100PPM, pH 5.42, alkalinity 30
0, organic matter 78.5%, inorganic matter 21.5%) 200 each
g, the culture solution B of the present invention was added and stirred at the respective addition amounts shown in Table 2, and after standing for 20 minutes, 200 PPM (converted to DRY) of a cationic polymer flocculant (Konan Floc K-53P) was added. No. It filtered with 1 filter paper and the dehydrated cake was obtained. This dehydrated cake was collected in a 300 ml Erlenmeyer flask, sealed and kept at 40 ° C., and the odorous component concentrations after 4 hours and 24 hours were measured.

When the culture solution was added, the filtration rate was improved as compared with the case where the culture solution was not added.

[0017]

[Table 2]

[0018]

As described above, the addition of the culture solution of the present invention to sludge can fundamentally significantly reduce the generation of malodorous substances.

Further, since it is derived from a natural product, it has good biodegradability and there is no fear of adverse effects on the subsequent steps or secondary pollution.

It also has the effect of improving the solid-liquid separation of sludge and reducing the water content of the dehydrated cake. In addition, it is also useful for breaking down oil and fat in oil-containing wastewater.

Since the generation of corrosive substances such as hydrogen sulfide is suppressed, the corrosion of iron, concrete, etc. is prevented, which is very effective for the maintenance of the plant. Further, it is very useful in terms of safety and hygiene because it prevents the generation of harmful gas in the working environment.

The culture broth according to the present invention may be used after being subjected to treatments such as concentration, salt folding and solvent extraction, and a preservative may be mixed. It is also possible to fix the culture solution according to the present invention on a carrier, and bring the carrier into contact with a malodorous substance to deodorize it.

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Claims (1)

[Claims] 1. A deodorant comprising a culture solution containing a metabolite of a microorganism consisting of at least one or more species selected from Pseudomonas putida and Pseudomonas fluorescens belonging to the genus Pseudomonas.