CN103468619A - Microbial preparation for purifying air - Google Patents
Microbial preparation for purifying air Download PDFInfo
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- CN103468619A CN103468619A CN2013104437944A CN201310443794A CN103468619A CN 103468619 A CN103468619 A CN 103468619A CN 2013104437944 A CN2013104437944 A CN 2013104437944A CN 201310443794 A CN201310443794 A CN 201310443794A CN 103468619 A CN103468619 A CN 103468619A
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- bacillus pumilus
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- faecium
- pseudomonas putida
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention provides a microbial preparation for purifying air. By utilizing the synergic effects of streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, the microbial preparation is capable of effectively reducing concentration of stink comprising ammonia gas, hydrogen sulfide and the like in the air at toilets, culturing farms, dumps and the like, and inhibiting stink-producing elements, so that air quality and human living environment can be substantially improved. A production method provided by the invention is simple, is applicable to majority of conventional fermentation technologies and equipment, and is suitable for large-scale popularization and application.
Description
Technical field
The present invention relates to technical field of microbe application, be specifically related to a kind of microbial preparation purified air and application thereof.
Background technology
Odor pollution is one of seven universally acknowledged overall situation public hazards.Common odorant pollutant has ammonia, Trimethylamine 99, hydrogen sulfide, thiomethyl alcohol, methyl disulfide, dithiocarbonic anhydride and vinylbenzene etc., be mainly derived from industrial and agricultural production department and people's life, such as husbandry production with in processing, the excrement of generation is smelly, fish is smelly, corruption is smelly, decayed fruit is smelly, edible wild herbs are smelly etc.The sulfide produced in petrochemical production process, hydro carbons, benzene class, phenols and Ammonia and tar, pitch steam, ammonia and various organic solvent, and the stench of urban public utilities.The odorant pollutant of high density has direct harm to HUMAN HEALTH.
At present, can be divided into chemical deodorizing and biological deodorizing two broad aspect to the stench removal methods, the chemical deodorizing principle is, by outer source strength oxidizing substance, the stench composition is carried out to oxidative degradation, closely eliminate stench, though it is with strong points, instant effect, but its range of application is little, while running into the complex effluvium gas ingredients, deodorizing usefulness will lose efficacy, and after using the chemical deodorizing agent, residual chemical substance tends to cause secondary pollution.The biological deodorizing principle is to remove stench by external source function bacterial classification or biological enzyme to the direct degraded of stench or to the inhibition of producing the stench microorganism.The characteristics such as biological deodorizing can be eliminated stench lastingly, and non-secondary pollution is easy to use, with low cost, become the study hotspot that stench is controlled, and finds effective, green biological deodorizing product and method, becomes the active demand of present society.
Summary of the invention
The present invention, for solving the prior art problem, provides a kind of odorant pollutant in air of can removing, and then the complex microorganism preparations purified air.Said preparation, by the collaborative promoter action of multiple-microorganism, can effectively suppress to produce smelly element, the degraded odorant pollutant, thus the concentration of minimizing obnoxious flavour reduces odor concentration.
The applicant finds by long-term screening study, can produce synergy between thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium, effectively reduce the concentration of the foul smell such as Ammonia in Air gas and hydrogen sulfide, thereby facilitate the present invention.
One aspect of the present invention provides a kind of deodorizing microorganism preparation, by thermophilus streptococcus (
streptococcus thermophilus), pseudomonas putida (
pseudomonas putida), Rhodopseudomonas palustris (
rhodopseudomonas palustris), bacillus pumilus (
bacillus pumilus) and faecium (
enterococcus faecium) form.
The preferred thermophilus streptococcus CGMCC1.3996 of each component, pseudomonas putida CGMCC1.2309, Rhodopseudomonas palustris CGMCC1.2349, bacillus pumilus CGMCC1.8112 and faecium CGMCC1.2136 in above-mentioned deodorizing microorganism preparation.
In above-mentioned deodorizing microorganism preparation, each component and content thereof are respectively: thermophilus streptococcus 40-60 part, pseudomonas putida 35-40 part, Rhodopseudomonas palustris 18-30 part, bacillus pumilus 25-50 part and faecium 45-80 part.
In above-mentioned deodorizing microorganism preparation, the content of each component is preferably respectively: 55 parts of 45 parts of thermophilus streptococcuses, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustriss, 40 parts of bacillus pumilus and faeciums.
The present invention provides the preparation method of above-mentioned deodorizing microorganism preparation on the other hand, comprises the steps:
1) thermophilus streptococcus CGMCC1.3996, pseudomonas putida CGMCC1.2309, Rhodopseudomonas palustris CGMCC1.2349, bacillus pumilus CGMCC1.8112 and faecium CGMCC1.2136 are adopted respectively to the high density liquid fermentation, by after the fermented liquid freeze-drying, make gemma content up to 10
10-10
11the hyperconcentration bacterium powder of CFU/g;
2) hyperconcentration bacterium powder is mixed with to complex microorganism preparations according to thermophilus streptococcus 40-60 part, pseudomonas putida 35-40 part, Rhodopseudomonas palustris 18-30 part, bacillus pumilus 25-50 part and faecium 45-80 part, is solid deodorizing microorganism preparation;
3) get step 2) microbial preparation that makes, add by the 1-5% mass volume ratio sodium citrate solution that concentration is 8-10%, mix, obtain liquid deodorant microbial preparation of the present invention.
The present invention provides the application of above-mentioned deodorizing microorganism preparation in purifying air on the other hand.
Aforesaid liquid deodorizing microorganism preparation is sprayed on to the high places of malodorant concentration such as lavatory, plant, refuse tip, sprays 2-3 time weekly, each consumption 100-200 mL/ m
3.
beneficial effect
Complex microorganism preparations provided by the invention is by the synergy between thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium, can effectively reduce the concentration of the foul smell such as the airborne ammonia in the places such as lavatory, plant, refuse tip and hydrogen sulfide, suppress to produce smelly element, thereby significantly improve Air quality and people's living environment.Production method provided by the invention is simple, is adapted to the existing zymotechnique of great majority and equipment, is conducive to large-scale promotion application.
Embodiment
The all ingredients the present invention relates to and equipment, all can be selected from commercially available any one; The present invention relates in bacterial strain thermophilus streptococcus purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.3996; Pseudomonas putida is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.2309; Rhodopseudomonas palustris is Qingdao Weilan Biology Group Co., Ltd.'s patented strain, deposit number CGMCC1.2349; Bacillus pumilus is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.8112; Faecium is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.2136.
Below in conjunction with specific embodiment, further set forth the present invention.
embodiment 1
A kind of deodorizing microorganism preparation, thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium, consist of, each component and content thereof are respectively: 45 parts of 40 parts of thermophilus streptococcuses, 40 parts of pseudomonas putidas, 30 parts of Rhodopseudomonas palustriss, 25 parts of bacillus pumilus and faeciums.
The preparation method of above-mentioned deodorizing microorganism preparation, comprise the steps:
1) thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium are adopted respectively to the high density liquid fermentation, by after the fermented liquid freeze-drying, make gemma content up to 10
10-10
11the hyperconcentration bacterium powder of CFU/g;
2) hyperconcentration bacterium powder is mixed with to complex microorganism preparations according to 45 parts of 40 parts of thermophilus streptococcuses, 40 parts of pseudomonas putidas, 30 parts of Rhodopseudomonas palustriss, 25 parts of bacillus pumilus and faeciums, is solid deodorizing microorganism preparation;
3) get step 2) microbial preparation that makes, add by 1% mass volume ratio the sodium citrate solution that concentration is 10%, mix, obtain liquid deodorant microbial preparation of the present invention.
embodiment 2
A kind of deodorizing microorganism preparation, thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium, consist of, each component and content thereof are respectively: 80 parts of 60 parts of thermophilus streptococcuses, 35 parts of pseudomonas putidas, 18 parts of Rhodopseudomonas palustriss, 50 parts of bacillus pumilus and faeciums.
The preparation method of above-mentioned deodorizing microorganism preparation, comprise the steps:
1) thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium are adopted respectively to the high density liquid fermentation, by after the fermented liquid freeze-drying, make gemma content up to 10
10-10
11the hyperconcentration bacterium powder of CFU/g;
2) hyperconcentration bacterium powder is mixed with to complex microorganism preparations according to 80 parts of 60 parts of thermophilus streptococcuses, 35 parts of pseudomonas putidas, 18 parts of Rhodopseudomonas palustriss, 50 parts of bacillus pumilus and faeciums, is solid deodorizing microorganism preparation;
3) get step 2) microbial preparation that makes, add by 5% mass volume ratio the sodium citrate solution that concentration is 8%, mix, obtain liquid deodorant microbial preparation of the present invention.
embodiment 3
A kind of deodorizing microorganism preparation, thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium, consist of, each component and content thereof are respectively: 55 parts of 45 parts of thermophilus streptococcuses, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustriss, 40 parts of bacillus pumilus and faeciums.
The preparation method of above-mentioned deodorizing microorganism preparation, comprise the steps:
1) thermophilus streptococcus, pseudomonas putida, Rhodopseudomonas palustris, bacillus pumilus and faecium are adopted respectively to the high density liquid fermentation, by after the fermented liquid freeze-drying, make gemma content up to 10
10-10
11the hyperconcentration bacterium powder of CFU/g;
2) hyperconcentration bacterium powder is mixed with to complex microorganism preparations according to 55 parts of 45 parts of thermophilus streptococcuses, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustriss, 40 parts of bacillus pumilus and faeciums, is solid deodorizing microorganism preparation;
3) get step 2) microbial preparation that makes, add by 4% mass volume ratio the sodium citrate solution that concentration is 9%, mix, obtain liquid deodorant microbial preparation of the present invention.
the application of embodiment 4 deodorizing microorganism preparations
1, add 100g solid-state deodorizing microorganism preparation of the present invention in the beaker that the fresh pig manure of 1000g is housed (2L), and after ight soil mixes, the preservative film sealed beaker; Do the contrast that does not add microbial inoculum, every group of experiment arranges three repetitions, measures stench intensity, ammonia concentration and concentration of hydrogen sulfide after 7 days simultaneously, and measurement result is as shown in table 1 below:
| Testing index | Control group | Add the microbial preparation group | Reduced rate |
| Odor strength | 297312 | 178387 | 40.0% |
| Ammonia concentration | 42.3 | 21.4 | 49.4% |
| Concentration of hydrogen sulfide | 156.1 | 77.2 | 50.5% |
As can be seen from Table 1, after adding deodorizing microorganism preparation of the present invention, the ammonia that ight soil produces and the concentration of hydrogen sulfide have reduced respectively 49.4% and 50.5%, significantly reduce odor strength, illustrate that microbial preparation of the present invention has good deodorizing effect, can effectively improve Air quality.
2, choose in the other life refuse collecting of Li Cunhe and deposit a little as test site, detect respectively spray liquid deodorant microbial preparation of the present invention before and sprinkling (sprinkling consumption 150g/ m
3) rear the 3rd day airborne stench intensity, ammonia concentration and concentration of hydrogen sulfide, measurement result is as shown in table 2 below:
| Testing index | Before sprinkling | After sprinkling | Reduced rate |
| Odor strength | 312410 | 164371 | 47.4% |
| Ammonia concentration | 56.1 | 27.8 | 50.4% |
| Concentration of hydrogen sulfide | 152.8 | 79.1 | 48.2% |
As can be seen from Table 2, after spraying deodorizing microorganism of the present invention, in the refuse tip air, odor strength significantly reduces by 47.4%, wherein ammonia concentration has reduced by 50.4%, concentration of hydrogen sulfide has reduced by 48.2%, the deodorizing effect that microbial preparation of the present invention is described is remarkable, can effectively reduce the concentration of repugnant substance in air, is conducive to better people's living environment.
Claims (6)
1. a deodorizing microorganism preparation, by by thermophilus streptococcus (Streptococcus thermophilus), pseudomonas putida (
pseudomonas putida), Rhodopseudomonas palustris (
rhodopseudomonas palustris), bacillus pumilus (
bacillus pumilus) and faecium (
enterococcus faecium) form.
2. the described deodorizing microorganism preparation of claim 1, be comprised of thermophilus streptococcus CGMCC1.3996, pseudomonas putida CGMCC1.2309, Rhodopseudomonas palustris CGMCC1.2349, bacillus pumilus CGMCC1.8112 and faecium CGMCC1.2136.
3. deodorizing microorganism preparation as claimed in claim 1 or 2, it is characterized in that, the content of each component is respectively: thermophilus streptococcus 40-60 part, pseudomonas putida 35-40 part, Rhodopseudomonas palustris 18-30 part, bacillus pumilus 25-50 part and faecium 45-80 part.
4. deodorizing microorganism preparation as claimed in claim 3, is characterized in that, the content of each component is respectively: 55 parts of 45 parts of thermophilus streptococcuses, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustriss, 40 parts of bacillus pumilus and faeciums.
5. the application of the described deodorizing microorganism preparation of claim 1 in purifying air.
6. the application of deodorizing microorganism preparation as claimed in claim 5, is characterized in that, the sprinkling consumption of deodorizing microorganism preparation is 100-200g/m
3, use 2-3 time weekly.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104946544A (en) * | 2015-06-30 | 2015-09-30 | 防城港市盛吉科技有限公司 | Microbial deodorizing inoculant |
| CN106036320A (en) * | 2016-06-16 | 2016-10-26 | 辽宁省微生物科学研究院 | Method for preparing mushroom mycelial fermentation broth original drink |
| CN106967640A (en) * | 2017-04-11 | 2017-07-21 | 上海莱泰生物科技有限公司 | A kind of preparation method of deodorizing microorganism microbial inoculum |
| CN111548960A (en) * | 2020-05-13 | 2020-08-18 | 深圳市东荣生物科技有限责任公司 | Compound microbial preparation for deodorization |
| CN111893071A (en) * | 2020-08-12 | 2020-11-06 | 浙江丹靓环境工程有限公司 | Soil deodorization microbial preparation and application thereof |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
| CN112608859A (en) * | 2020-12-17 | 2021-04-06 | 新疆河润水业有限责任公司 | Bacteriostatic and odor-removing preparation based on microorganisms and preparation method and application thereof |
| CN114891662A (en) * | 2022-03-05 | 2022-08-12 | 福建亮洁环境服务有限公司 | Pseudomonas putida and application thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104946544A (en) * | 2015-06-30 | 2015-09-30 | 防城港市盛吉科技有限公司 | Microbial deodorizing inoculant |
| CN106036320A (en) * | 2016-06-16 | 2016-10-26 | 辽宁省微生物科学研究院 | Method for preparing mushroom mycelial fermentation broth original drink |
| CN106967640A (en) * | 2017-04-11 | 2017-07-21 | 上海莱泰生物科技有限公司 | A kind of preparation method of deodorizing microorganism microbial inoculum |
| CN111548960A (en) * | 2020-05-13 | 2020-08-18 | 深圳市东荣生物科技有限责任公司 | Compound microbial preparation for deodorization |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
| CN111893071A (en) * | 2020-08-12 | 2020-11-06 | 浙江丹靓环境工程有限公司 | Soil deodorization microbial preparation and application thereof |
| CN112608859A (en) * | 2020-12-17 | 2021-04-06 | 新疆河润水业有限责任公司 | Bacteriostatic and odor-removing preparation based on microorganisms and preparation method and application thereof |
| CN114891662A (en) * | 2022-03-05 | 2022-08-12 | 福建亮洁环境服务有限公司 | Pseudomonas putida and application thereof |
| CN114891662B (en) * | 2022-03-05 | 2023-11-24 | 闽江学院 | Pseudomonas putida and application thereof |
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