CN106967640A - A kind of preparation method of deodorizing microorganism microbial inoculum - Google Patents

A kind of preparation method of deodorizing microorganism microbial inoculum Download PDF

Info

Publication number
CN106967640A
CN106967640A CN201710231159.8A CN201710231159A CN106967640A CN 106967640 A CN106967640 A CN 106967640A CN 201710231159 A CN201710231159 A CN 201710231159A CN 106967640 A CN106967640 A CN 106967640A
Authority
CN
China
Prior art keywords
content
preparation
water
composite bacteria
microbial inoculum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710231159.8A
Other languages
Chinese (zh)
Inventor
徐苏愉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANGHAI LAITAI BIOTECHNOLOGY CO Ltd
Original Assignee
SHANGHAI LAITAI BIOTECHNOLOGY CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHANGHAI LAITAI BIOTECHNOLOGY CO Ltd filed Critical SHANGHAI LAITAI BIOTECHNOLOGY CO Ltd
Priority to CN201710231159.8A priority Critical patent/CN106967640A/en
Publication of CN106967640A publication Critical patent/CN106967640A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/01—Deodorant compositions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2251/00—Reactants
    • B01D2251/95—Specific microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/30—Sulfur compounds
    • B01D2257/304—Hydrogen sulfide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/40—Nitrogen compounds
    • B01D2257/406—Ammonia
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
    • B01D2257/704—Solvents not covered by groups B01D2257/702 - B01D2257/7027
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/90—Odorous compounds not provided for in groups B01D2257/00 - B01D2257/708
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/91—Bacteria; Microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2258/00—Sources of waste gases
    • B01D2258/02—Other waste gases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of preparation method of deodorizing microorganism microbial inoculum, belong to deodorant field.Comprise the following steps：（1）The preparation of composite bacteria；（2）The preparation of culture medium；（3）The preparation of composite bacteria agent stoste；（4）The preparation of fermenation raw liquid；（5）The generation of deodorizing microorganism microbial inoculum.By a variety of strain composite deodurizings, multi-cultur es cooperate, comprehensive regulation stench, can reach deodorizing effect more more preferable than single bacterium kind.

Description

A kind of preparation method of deodorizing microorganism microbial inoculum
Technical field
The present invention relates to a kind of preparation method of deodorizing microorganism microbial inoculum, belong to deodorant field.
Background technology
China's domestic waste year amount of clearing is about 200,000,000 tons, in addition to small part burning, compost or recycling, its Remaining and more than 70% be transported to landfill yard and be disposed.Rubbish during the stacking of landfill yard, handling, tiling, compacting etc., because For the rotten decomposition of organic matter, odor pollution will be inevitably produced.Foul gas is mainly divided into 5 classes by its component：① Sulfur-containing compound, such as H2S、SO2, mercaptan, thioether etc.；2. nitrogen-containing compound, such as ammonia, amine；3. halogen and derivative, such as Halogenated hydrocarbons etc.；4. hydro carbons and aromatic hydrocarbon；5. oxygen-bearing organic matter, such as alcohol, phenol, aldehyde, ketone, organic acid.Stench can both be seriously endangered Health, will produce secondary pollution again.At present, for the rubbish in landfill yard, needing badly a kind of can remove odor pollution Deodorant.
The content of the invention
The technical problems to be solved by the invention are：A kind of preparation method of deodorizing microorganism microbial inoculum is provided, it can A kind of efficient deodorizing microorganism microbial inoculum is prepared, the problem of organic matter rubbish rots to produce stench during decomposition is solved.
The technical problems to be solved by the invention take following technical scheme to realize：
A kind of preparation method of deodorizing microorganism microbial inoculum, comprises the following steps：
（1）The preparation of composite bacteria：By the pseudomonas putida (Pseudomonas putida) after activation, bacillus coagulans (Bacillus coagulans), Bacillus cercus (Bacillus cereus) and water are configured to composite bacteria, described compound Pseudomonas putida bacterial content, which accounts for 25~30wt%, bacillus coagulans content and accounts for 20~30wt%, Bacillus cercus, in strain contains Amount accounts for 10~20wt%, and surplus is water；
（2）The preparation of culture medium：Peptone, glucose, maltose and water are hybridly prepared into culture medium；
（3）The preparation of composite bacteria agent stoste：By step（1）Composite bacteria be inoculated into step（2）Culture medium in, wherein compound The weight proportion scope of strain and culture medium is 1:99~10:90, under the conditions of 37 DEG C, shaking table culture 24h obtains composite bacteria agent Stoste；
（4）The preparation of fermenation raw liquid：Prepare molasses and vitamin solution, molasses are sterilized 20min, dimension life under the conditions of 121 DEG C Plain solution is by biotin, vitamin B12With water composition, vitamin solution is sterilized 15min under the conditions of 110 DEG C, by molasses, dimension Raw element solution and step（3）Composite bacteria agent stoste and water be configured to molasses content in fermenation raw liquid, the fermenation raw liquid and be 25wt%, vitamin solution content is 0.2wt%, and composite bacteria agent stoste content is 20~30wt%, and surplus is water；
（5）The generation of deodorizing microorganism microbial inoculum：Sealed fermenter is sterilized 20min, by step（4）Fermenation raw liquid be put into 5 tons Fill in sealed fermenter, fermentation obtains deodorizing microorganism microbial inoculum in 7 days under the conditions of 30 DEG C.
As preferred embodiment, peptone content is that 0.8wt%, glucose content are that 1wt%, maltose contain in the culture medium Measure as 0.5wt%, surplus is water.
As preferred embodiment, biotin content is 2wt%, vitamin B in the vitamin solution12Content is 12wt%, remaining Measure as water.
The beneficial effects of the invention are as follows：
(1) pseudomonas putida is used for the environmental contaminants such as degradation of phenol；
(2) bacillus coagulans generates lactic acid in metabolic process, for harmful gas such as the ammonia in environment of degrading, hydrogen sulfide Body, and can effectively suppress the growth of harmful bacteria, generation is unfavorable for the weakly acidic condition of corrupt bacteria growing in junk-heap body；
(3) Bacillus cercus produces the materials such as a variety of enzymes, lactic acid, propionic acid, acetic acid, it is suppressed that Escherichia coli are to protein Decomposition and the growth of Escherichia coli, so as to reduce the concentration of ammonia and amine, improve environment；
（4）By a variety of strain composite deodurizings, multi-cultur es cooperate, comprehensive regulation stench, can reach more preferable than single bacterium kind Deodorizing effect.
Embodiment
In order to which technological means, creation characteristic, reached purpose and effect to the present invention are easy to understand, with reference to reality Example is applied, the present invention is expanded on further.
A kind of preparation method of deodorizing microorganism microbial inoculum, comprises the following steps：
（1）The preparation of composite bacteria：By the pseudomonas putida (Pseudomonas putida) after activation, bacillus coagulans (Bacillus coagulans), Bacillus cercus (Bacillus cereus) and water are configured to composite bacteria, composite bacteria Middle Pseudomonas putida bacterial content, which accounts for 25~30wt%, bacillus coagulans content and accounts for 20~30wt%, Bacillus cercus content, to be accounted for 10~20wt%, surplus is water；
（2）The preparation of culture medium：Peptone, glucose, maltose and water are hybridly prepared into peptone in culture medium, culture medium Content is that 0.8wt%, glucose content are that 1wt%, maltose content are 0.5wt%, and surplus is water；
（3）The preparation of composite bacteria agent stoste：By step（1）Composite bacteria be inoculated into step（2）Culture medium in, wherein compound The weight proportion scope of strain and culture medium is 1:99~10:90, under the conditions of 37 DEG C, shaking table culture 24h obtains composite bacteria agent Stoste；
（4）The preparation of fermenation raw liquid：Prepare molasses and vitamin solution, molasses are sterilized 20min, dimension life under the conditions of 121 DEG C Plain solution is by biotin, vitamin B12Constituted with water, biotin content is 2wt%, vitamin B in vitamin solution12Content For 12wt%, surplus is water, vitamin solution is sterilized 15min under the conditions of 110 DEG C, by molasses, vitamin solution, Yi Jibu Suddenly（3）Composite bacteria agent stoste and water to be configured in fermenation raw liquid, fermenation raw liquid molasses content be 25wt%, vitamin solution contains Measure as 0.2wt%, composite bacteria agent stoste content is 20~30wt%, and surplus is water；
（5）The generation of deodorizing microorganism microbial inoculum：Sealed fermenter is sterilized 20min, by step（4）Fermenation raw liquid be put into 5 tons Fill in sealed fermenter, fermentation obtains deodorizing microorganism microbial inoculum in 7 days under the conditions of 30 DEG C.
Above-mentioned pseudomonas putida (Pseudomonas putida), bacillus coagulans (Bacillus Coagulans), Bacillus cercus (Bacillus cereus) is by China General Microbiological culture presevation administrative center preservation, Bacterium numbering is respectively 1.3096,1.3220,1.3760.
Embodiment 1
A kind of preparation method of deodorizing microorganism microbial inoculum, comprises the following steps：
（1）The preparation of composite bacteria：By the pseudomonas putida (Pseudomonas putida) after activation, bacillus coagulans (Bacillus coagulans), Bacillus cercus (Bacillus cereus) and water are configured to composite bacteria, composite bacteria Middle Pseudomonas putida bacterial content, which accounts for 25wt%, bacillus coagulans content and accounts for 20wt%, Bacillus cercus content, accounts for 10wt%, remaining Measure as water；
（2）The preparation of culture medium：Peptone, glucose, maltose and water are hybridly prepared into peptone in culture medium, culture medium Content is that 0.8wt%, glucose content are that 1wt%, maltose content are 0.5wt%, and surplus is water；
（3）The preparation of composite bacteria agent stoste：By step（1）Composite bacteria be inoculated into step（2）Culture medium in, wherein compound The weight proportion of strain and culture medium is 10:90, under the conditions of 37 DEG C, shaking table culture 24h obtains composite bacteria agent stoste；
（4）The preparation of fermenation raw liquid：Prepare molasses and vitamin solution, molasses are sterilized 20min, dimension life under the conditions of 121 DEG C Plain solution is by biotin, vitamin B12Constituted with water, biotin content is 2wt%, vitamin B in vitamin solution12Content For 12wt%, surplus is water, vitamin solution is sterilized 15min under the conditions of 110 DEG C, by molasses, vitamin solution, Yi Jibu Suddenly（3）Composite bacteria agent stoste and water to be configured in fermenation raw liquid, fermenation raw liquid molasses content be 25wt%, vitamin solution contains Measure as 0.2wt%, composite bacteria agent stoste content is 30wt%, and surplus is water；
（5）The generation of deodorizing microorganism microbial inoculum：Sealed fermenter is sterilized 20min, by step（4）Fermenation raw liquid be put into 5 tons Fill in sealed fermenter, fermentation obtains deodorizing microorganism microbial inoculum in 7 days under the conditions of 30 DEG C.
The deodorizing microorganism microbial inoculum being prepared from according to embodiment 1, its deodorizing effect detection is as follows：
The rubbish heap of 1 ton of weight is chosen in landfill yard, the junk-heap body odor concentration without deodorization is first detected, then with 1:30 is dilute 0.8 kilogram of dilution proportion microbial deoderizer stoste is released, 24 kilograms of deodorizing microorganism dilution is obtained, 24 kilograms of dilutions are equal It is even to be sprayed into junk-heap body, deodorizing effect is detected after half an hour.
Through third party testing agency --- SGS Tongbiao Standard Technology Service Co., Ltd is detected：Spray microbial deodorant bacterium After agent, odor concentration drops to 60 by 486.
Embodiment 2
A kind of preparation method of deodorizing microorganism microbial inoculum, comprises the following steps：
（1）The preparation of composite bacteria：By the pseudomonas putida (Pseudomonas putida) after activation, bacillus coagulans (Bacillus coagulans), Bacillus cercus (Bacillus cereus) and water are configured to composite bacteria, composite bacteria Middle Pseudomonas putida bacterial content, which accounts for 28wt%, bacillus coagulans content and accounts for 25wt%, Bacillus cercus content, accounts for 15wt%, remaining Measure as water；
（2）The preparation of culture medium：Peptone, glucose, maltose and water are hybridly prepared into peptone in culture medium, culture medium Content is that 0.8wt%, glucose content are that 1wt%, maltose content are 0.5wt%, and surplus is water；
（3）The preparation of composite bacteria agent stoste：By step（1）Composite bacteria be inoculated into step（2）Culture medium in, wherein compound The weight proportion of strain and culture medium is 5:95, under the conditions of 37 DEG C, shaking table culture 24h obtains composite bacteria agent stoste；
（4）The preparation of fermenation raw liquid：Prepare molasses and vitamin solution, molasses are sterilized 20min, dimension life under the conditions of 121 DEG C Plain solution is by biotin, vitamin B12Constituted with water, biotin content is 2wt%, vitamin B in vitamin solution12Content For 12wt%, surplus is water, vitamin solution is sterilized 15min under the conditions of 110 DEG C, by molasses, vitamin solution, Yi Jibu Suddenly（3）Composite bacteria agent stoste and water to be configured in fermenation raw liquid, fermenation raw liquid molasses content be 25wt%, vitamin solution contains Measure as 0.2wt%, composite bacteria agent stoste content is 25wt%, and surplus is water；
（5）The generation of deodorizing microorganism microbial inoculum：Sealed fermenter is sterilized 20min, by step（4）Fermenation raw liquid be put into 5 tons Fill in sealed fermenter, fermentation obtains deodorizing microorganism microbial inoculum in 7 days under the conditions of 30 DEG C.
The deodorizing microorganism microbial inoculum being prepared from according to embodiment 2, its deodorizing effect detection is as follows：
The rubbish heap of 1 ton of weight is chosen in landfill yard, the junk-heap body odor concentration without deodorization is first detected, then with 1:30 is dilute 0.8 kilogram of dilution proportion microbial deoderizer stoste is released, 24 kilograms of deodorizing microorganism dilution is obtained, 24 kilograms of dilutions are equal It is even to be sprayed into junk-heap body, deodorizing effect is detected after half an hour.
Through third party testing agency --- SGS Tongbiao Standard Technology Service Co., Ltd is detected：Spray microbial deodorant bacterium After agent, odor concentration drops to 59 by 483.
Embodiment 3
A kind of preparation method of deodorizing microorganism microbial inoculum, comprises the following steps：
（1）The preparation of composite bacteria：By the pseudomonas putida (Pseudomonas putida) after activation, bacillus coagulans (Bacillus coagulans), Bacillus cercus (Bacillus cereus) and water are configured to composite bacteria, composite bacteria Middle Pseudomonas putida bacterial content, which accounts for 30wt%, bacillus coagulans content and accounts for 30wt%, Bacillus cercus content, accounts for 20wt%, remaining Measure as water；
（2）The preparation of culture medium：Peptone, glucose, maltose and water are hybridly prepared into peptone in culture medium, culture medium Content is that 0.8wt%, glucose content are that 1wt%, maltose content are 0.5wt%, and surplus is water；
（3）The preparation of composite bacteria agent stoste：By step（1）Composite bacteria be inoculated into step（2）Culture medium in, wherein compound The weight proportion of strain and culture medium is 2:98, under the conditions of 37 DEG C, shaking table culture 24h obtains composite bacteria agent stoste；
（4）The preparation of fermenation raw liquid：Prepare molasses and vitamin solution, molasses are sterilized 20min, dimension life under the conditions of 121 DEG C Plain solution is by biotin, vitamin B12Constituted with water, biotin content is 2wt%, vitamin B in vitamin solution12Content For 12wt%, surplus is water, vitamin solution is sterilized 15min under the conditions of 110 DEG C, by molasses, vitamin solution, Yi Jibu Suddenly（3）Composite bacteria agent stoste and water to be configured in fermenation raw liquid, fermenation raw liquid molasses content be 25wt%, vitamin solution contains Measure as 0.2wt%, composite bacteria agent stoste content is 30wt%, and surplus is water；
（5）The generation of deodorizing microorganism microbial inoculum：Sealed fermenter is sterilized 20min, by step（4）Fermenation raw liquid be put into 5 tons Fill in sealed fermenter, fermentation obtains deodorizing microorganism microbial inoculum in 7 days under the conditions of 30 DEG C.
The deodorizing microorganism microbial inoculum being prepared from according to embodiment 3, its deodorizing effect detection is as follows：
The rubbish heap of 1 ton of weight is chosen in landfill yard, the junk-heap body odor concentration without deodorization is first detected, then with 1:30 is dilute 0.8 kilogram of dilution proportion microbial deoderizer stoste is released, 24 kilograms of deodorizing microorganism dilution is obtained, 24 kilograms of dilutions are equal It is even to be sprayed into junk-heap body, deodorizing effect is detected after half an hour.
Through third party testing agency --- SGS Tongbiao Standard Technology Service Co., Ltd is detected：Spray microbial deodorant bacterium After agent, odor concentration drops to 61 by 488.
Deodorization principle：Pseudomonas putida is used for the environmental contaminants such as degradation of phenol；Bacillus coagulans is in metabolic process Middle generation lactic acid, for pernicious gases such as the ammonia in environment of degrading, hydrogen sulfide, and can effectively suppress the growth of harmful bacteria, Generation is unfavorable for the weakly acidic condition of corrupt bacteria growing in junk-heap body；Bacillus cercus produce a variety of enzymes, lactic acid, propionic acid, The materials such as acetic acid, it is suppressed that Escherichia coli are to the decomposition of protein and the growth of Escherichia coli, so as to reduce the dense of ammonia and amine Degree, improves environment；By a variety of strain composite deodurizings, multi-cultur es cooperate, comprehensive regulation stench, can reach than single bacterium kind More preferable deodorizing effect.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, without departing from the spirit and scope of the present invention, this hair Bright also to have various changes and modifications, these changes and improvements all fall within the protetion scope of the claimed invention.Application claims Protection domain is by appended claims and its equivalent thereof.

Claims (3)

1. a kind of preparation method of deodorizing microorganism microbial inoculum, it is characterised in that comprise the following steps：
（1）The preparation of composite bacteria：By the pseudomonas putida (Pseudomonas putida) after activation, bacillus coagulans (Bacillus coagulans), Bacillus cercus (Bacillus cereus) and water are configured to composite bacteria, described compound Pseudomonas putida bacterial content, which accounts for 25~30wt%, bacillus coagulans content and accounts for 20~30wt%, Bacillus cercus, in strain contains Amount accounts for 10~20wt%, and surplus is water；
（2）The preparation of culture medium：Peptone, glucose, maltose and water are hybridly prepared into culture medium；
（3）The preparation of composite bacteria agent stoste：By step（1）Composite bacteria be inoculated into step（2）Culture medium in, wherein compound The weight proportion scope of strain and culture medium is 1:99~10:90, under the conditions of 37 DEG C, shaking table culture 24h obtains composite bacteria agent Stoste；
（4）The preparation of fermenation raw liquid：Prepare molasses and vitamin solution, molasses are sterilized 20min, dimension life under the conditions of 121 DEG C Plain solution is by biotin, vitamin B12With water composition, vitamin solution is sterilized 15min under the conditions of 110 DEG C, by molasses, dimension Raw element solution and step（3）Composite bacteria agent stoste and water be configured to molasses content in fermenation raw liquid, the fermenation raw liquid and be 25wt%, vitamin solution content is 0.2wt%, and composite bacteria agent stoste content is 20~30wt%, and surplus is water；
（5）The generation of deodorizing microorganism microbial inoculum：Sealed fermenter is sterilized 20min, by step（4）Fermenation raw liquid be put into 5 tons Fill in sealed fermenter, fermentation obtains deodorizing microorganism microbial inoculum in 7 days under the conditions of 30 DEG C.
2. a kind of preparation method of deodorizing microorganism microbial inoculum according to claim 1, it is characterised in that：Egg in the culture medium White peptone content is that 0.8wt%, glucose content are that 1wt%, maltose content are 0.5wt%, and surplus is water.
3. a kind of preparation method of deodorizing microorganism microbial inoculum according to claim 1, it is characterised in that：The vitamin solution Middle biotin content is 2wt%, vitamin B12Content is 12wt%, and surplus is water.
CN201710231159.8A 2017-04-11 2017-04-11 A kind of preparation method of deodorizing microorganism microbial inoculum Pending CN106967640A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710231159.8A CN106967640A (en) 2017-04-11 2017-04-11 A kind of preparation method of deodorizing microorganism microbial inoculum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710231159.8A CN106967640A (en) 2017-04-11 2017-04-11 A kind of preparation method of deodorizing microorganism microbial inoculum

Publications (1)

Publication Number Publication Date
CN106967640A true CN106967640A (en) 2017-07-21

Family

ID=59337165

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710231159.8A Pending CN106967640A (en) 2017-04-11 2017-04-11 A kind of preparation method of deodorizing microorganism microbial inoculum

Country Status (1)

Country Link
CN (1) CN106967640A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108102990A (en) * 2018-03-05 2018-06-01 河南师范大学 A kind of probiotics with deodorization functions and its preparation method and application
CN108404647A (en) * 2018-03-27 2018-08-17 湖南省蓝正微生物科技开发有限公司 Complex microorganism deodorant
CN109647178A (en) * 2018-12-25 2019-04-19 广东高龙环保科技有限公司 A kind of Organic Fertilizer Plants fermentation plant deodorization process
CN114891662A (en) * 2022-03-05 2022-08-12 福建亮洁环境服务有限公司 Pseudomonas putida and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005185658A (en) * 2003-12-26 2005-07-14 Kazunari Hashimoto Deodorizing agent, deodorizer using it, and deodorizing method
CN101671643A (en) * 2009-09-27 2010-03-17 上海创博生态工程有限公司 Method for preparing deodorizer by composite microorganism
CN101712937A (en) * 2008-10-06 2010-05-26 重庆尚美环保技术有限公司 Microbial agent used for cleaning freeness and deodorization of septic tank and preparation method thereof
CN103468619A (en) * 2013-09-26 2013-12-25 青岛蔚蓝生物集团有限公司 Microbial preparation for purifying air
CN103484413A (en) * 2013-10-14 2014-01-01 青岛蔚蓝生物集团有限公司 Pseudomonas putida strain and application thereof
CN103695331A (en) * 2013-06-27 2014-04-02 深圳市新宝环保能源科技有限公司 Bacillus coagulans and preparation method and application in biological deodorant thereof
CN106520627A (en) * 2016-12-07 2017-03-22 盐城复华环保产业开发有限公司 Microbial sludge deodorant and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005185658A (en) * 2003-12-26 2005-07-14 Kazunari Hashimoto Deodorizing agent, deodorizer using it, and deodorizing method
CN101712937A (en) * 2008-10-06 2010-05-26 重庆尚美环保技术有限公司 Microbial agent used for cleaning freeness and deodorization of septic tank and preparation method thereof
CN101671643A (en) * 2009-09-27 2010-03-17 上海创博生态工程有限公司 Method for preparing deodorizer by composite microorganism
CN103695331A (en) * 2013-06-27 2014-04-02 深圳市新宝环保能源科技有限公司 Bacillus coagulans and preparation method and application in biological deodorant thereof
CN103468619A (en) * 2013-09-26 2013-12-25 青岛蔚蓝生物集团有限公司 Microbial preparation for purifying air
CN103484413A (en) * 2013-10-14 2014-01-01 青岛蔚蓝生物集团有限公司 Pseudomonas putida strain and application thereof
CN106520627A (en) * 2016-12-07 2017-03-22 盐城复华环保产业开发有限公司 Microbial sludge deodorant and preparation method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108102990A (en) * 2018-03-05 2018-06-01 河南师范大学 A kind of probiotics with deodorization functions and its preparation method and application
CN108404647A (en) * 2018-03-27 2018-08-17 湖南省蓝正微生物科技开发有限公司 Complex microorganism deodorant
CN109647178A (en) * 2018-12-25 2019-04-19 广东高龙环保科技有限公司 A kind of Organic Fertilizer Plants fermentation plant deodorization process
CN114891662A (en) * 2022-03-05 2022-08-12 福建亮洁环境服务有限公司 Pseudomonas putida and application thereof
CN114891662B (en) * 2022-03-05 2023-11-24 闽江学院 Pseudomonas putida and application thereof

Similar Documents

Publication Publication Date Title
CN101671643B (en) Method for preparing deodorizer by composite microorganism
CN102228705B (en) Preparation method of biological deodourant
CN106967640A (en) A kind of preparation method of deodorizing microorganism microbial inoculum
CN102430142A (en) Composite ecological deodorant for stink gas control
CN111012938A (en) Deodorant for removing odor generated from garbage
CN106807228A (en) Special efficacy complex microorganism deodorant
CN112375716B (en) Garlic slice processing wastewater deodorization composite microbial inoculum
CN115181697A (en) Composite biological agent for deodorizing domestic garbage and preparation method thereof
CN109158403A (en) House refuse biology high temperature drying method for treating
CN106310923A (en) Biological active enzyme composite microbial deodorizer
CN106512707A (en) Microbial deodorizer with complex photosynthetic bacteria and bioactive enzyme
CN106344946A (en) Household fixed garbage efficient composite microbial deodorant
CN110591983A (en) Biological deodorant based on compound microorganisms and preparation method thereof
CN110777103B (en) Simple and easy and long-distance transportation rapid production method of biological deodorization bactericide
CN106310925A (en) Microorganism deodorant with brown alga extract, Houttuynia cordata and mint
CN106310926A (en) Microorganism deodorant with biological active enzyme, chrysanthemum and hornwort
CN106378000A (en) Deodorant with brown alga extract
CN106512706A (en) Microbial deodorant for municipal fixed garbage treatment
CN106362579A (en) Efficient compound microorganism deodorant used in waste-yard
CN106345279A (en) Microbial deodorizer containing compound photosynthetic bacteria
CN106422753A (en) Chrysanthemum active enzyme deodorant for treating solid garbage
CN106345284A (en) Microbial deodorizer containing bifidobacteria, osmanthus fragrans and bacterium activator
CN118667713A (en) Domestic garbage deodorizing composite biological microbial agent based on intestinal beneficial bacteria
KR20240136571A (en) Em fermented solution with reduced odor and method for treating food waste using the same
CN106362580A (en) Composite deodorant containing hornwort chrysanthemum flowers and active enzymes

Legal Events

Date Code Title Description
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information
CB02 Change of applicant information

Address after: 201914 Shanghai city Chongming District Hengsha Township Fumin branch 58, room 2346 (Shanghai Heng Tai Economic Development Zone)

Applicant after: Shanghai Laitai Biological Environmental Protection Technology Group Co., Ltd

Address before: 201914 Chongming County Hengsha township of Shanghai City Fumin branch 58, room 2346 (Shanghai Heng Tai Economic Development Zone)

Applicant before: SHANGHAI LAITAI BIOTECHNOLOGY Co.,Ltd.

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20170721