CN101671643B - Method for preparing deodorizer by composite microorganism - Google Patents
Method for preparing deodorizer by composite microorganism Download PDFInfo
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- CN101671643B CN101671643B CN2009101964982A CN200910196498A CN101671643B CN 101671643 B CN101671643 B CN 101671643B CN 2009101964982 A CN2009101964982 A CN 2009101964982A CN 200910196498 A CN200910196498 A CN 200910196498A CN 101671643 B CN101671643 B CN 101671643B
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- 238000000034 method Methods 0.000 title claims abstract description 14
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 235000013343 vitamin Nutrition 0.000 claims abstract description 16
- 239000011782 vitamin Substances 0.000 claims abstract description 16
- 229930003231 vitamin Natural products 0.000 claims abstract description 16
- 229940088594 vitamin Drugs 0.000 claims abstract description 16
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 16
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 9
- 239000011573 trace mineral Substances 0.000 claims abstract description 9
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 241000194017 Streptococcus Species 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 8
- 230000004151 fermentation Effects 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 241000233866 Fungi Species 0.000 claims description 12
- 235000013379 molasses Nutrition 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 241000186660 Lactobacillus Species 0.000 claims description 8
- 229940039696 lactobacillus Drugs 0.000 claims description 8
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 6
- 229930003756 Vitamin B7 Natural products 0.000 claims description 6
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 6
- 239000011790 ferrous sulphate Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- 229940045641 monobasic sodium phosphate Drugs 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 6
- 235000019156 vitamin B Nutrition 0.000 claims description 6
- 239000011720 vitamin B Substances 0.000 claims description 6
- 239000011735 vitamin B7 Substances 0.000 claims description 6
- 235000011912 vitamin B7 Nutrition 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 229960001763 zinc sulfate Drugs 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- 230000000813 microbial effect Effects 0.000 abstract description 6
- 238000000354 decomposition reaction Methods 0.000 abstract description 4
- 240000006024 Lactobacillus plantarum Species 0.000 abstract description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 abstract description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 3
- 101100476962 Drosophila melanogaster Sirup gene Proteins 0.000 abstract 2
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 abstract 2
- 239000003637 basic solution Substances 0.000 abstract 2
- 235000020357 syrup Nutrition 0.000 abstract 2
- 241001478307 Acidomonas Species 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000004321 preservation Methods 0.000 description 8
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000255925 Diptera Species 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 230000001877 deodorizing effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 241000256113 Culicidae Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 1
- 241000287107 Passer Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008953 bacterial degradation Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- JYYOBHFYCIDXHH-UHFFFAOYSA-N carbonic acid;hydrate Chemical compound O.OC(O)=O JYYOBHFYCIDXHH-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 239000010791 domestic waste Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 150000005826 halohydrocarbons Chemical class 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- -1 nitrogenous compound Chemical class 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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- 150000003384 small molecules Chemical class 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a method for preparing a deodorizer by a composite microorganism, which is characterized by comprising the following concrete steps: (1) inoculating a composite strain comprising saccharomyces cerevisiae, lactobacillus plantarum, streptococcus acidi and rhodopseudomonas palustris into a culture medium, culturing for 48 hours at the temperature of 30 DEG C and obtaining composite microbial agent basic solution; (2) sterilizing sirup, sterilizing a vitamin solution, putting fermentiation basic solution composed of the composite microbial agent raw liquid, the sirup, a trace element solution, the vitamin solution and the balance water into a closed tank, fermenting and obtaining the deodorizer. The invention can quicken the fermentation and the decomposition of organicsubstances in rubbish and reduce the volume of the rubbish stack in a short time, thus increasing the quantity of the total rubbish stacking quantity.
Description
Technical field
The present invention relates to a kind of method of utilizing complex microorganism to prepare reodorant, belong to environmental protection technical field.
Background technology
China's domestic waste year amount of clearing is about 1.4 hundred million tons, and except small part burning, compost or recycling, all the other are transported to landfill yard and dispose more than 70%.Rubbish the depositing of landfill yard, load and unload, in the processes such as tiling, compacting, because wherein organic rotten decomposition unavoidably will produce odor pollution.Foul gas can be divided into 5 classes by its composition: 1. sulfocompound, and as H
2S, SO
2, mercaptan, thioether etc.; 2. nitrogenous compound is as ammonia, amine, phthalein amine, cry and make an uproar etc.; 3. halogen and derivative, as grab gas, halohydrocarbon etc.; 4. hydro carbons and aromatic hydrocarbon; 5. oxygen-bearing organic matter is as alcohol, phenol, aldehyde, ketone, organic acid etc.Stench both can the serious harm human health, will produce secondary pollution again.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing complex microorganism to prepare reodorant.
To achieve these goals, technical scheme of the present invention provides a kind of method of utilizing complex microorganism to prepare reodorant, it is characterized in that, concrete steps are:
The first step: will be inoculated in the substratum by weight 1-10: 99-90 by the composite bacteria that yeast saccharomyces cerevisiae 25-30wt%, plant lactobacillus 20-30wt%, streptococcus acidi lactici 20-30wt% and Rhodopseudomonas palustris 10-20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, vitamin solution was sterilized 15 minutes at 110 ℃, composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution 0.8wt% that will be obtained by the first step, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed put into the inherent 30 ℃ of fermentations of closed tin and obtained reodorant in 15 days.
Further, described trace element solution is made up of the water of ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus.
Described vitamin solution is by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed.
Yeast saccharomyces cerevisiae in the described the first step (Sacharomyces cerevisiae), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC20065.Plant lactobacillus (Lactobacillus plantarum), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC11118.Streptococcus acidi lactici (Streptococcus lactis), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC10653.Rhodopseudomonas palustris (Rhodopseudomonas palustris), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC10649.Above-mentioned four kinds of bacterial classifications all can be bought.
Utilize complex microorganism group's comprehensive action, the organism in rubbish and the percolate, noxious pollutant, foul smell etc. can effectively be decomposed, reach the effect of deodorizing and harmless processing.Photosynthetic bacterium wherein is with H
2The S reduction is decomposed into S and H
2Thereby, reach the de-odorised purpose; By the anaerobically fermenting of microorganism, the organic macromolecule in the percolate is decomposed into small molecules acid and dissolved matter, and microorganism by breeding, is oxidized to carbonic acid gas and water with it again, finishes biodegradation process.Lactobacillus in the composite flora can suppress the movable and breeding of pathogenic bacterium, sclerotium bacteria, and harmful line bacterium is faded away;
Advantage of the present invention is:
1, quickens organic fermentation decomposition in the rubbish, reduce the volume of piling up rubbish at short notice, thereby increase total scrap heap quantity high-volume.
2, suppress the growth of putrefactive bacterium, improve organic decomposition approach, reduce ammonia and the burst size of hydrogen sulfide and the generation of Ammonia material, the biogas amount that also reduces bacterial degradation simultaneously and produced, thus reach the de-odorised purpose.
3, suppress the pupa hatching of mosquito, the long-range effect of multiplying that reduces mosquitos and flies that reaches reduces the chance that causes disease.
4, suppress the breeding of pathogenic microbes, prevent the generation of disease.
5, reduce the generation of water from diffusion, and also improved the water quality of water from diffusion, reduce wherein each kind of pollutant component greatly.
6, promote landfill area soilization and regenerated process.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 10: 90 by the composite bacteria that yeast saccharomyces cerevisiae 25wt%, plant lactobacillus 30wt%, streptococcus acidi lactici 25wt% and Rhodopseudomonas palustris 20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
Embodiment 2
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 1: 99 by the composite bacteria that yeast saccharomyces cerevisiae 30wt%, plant lactobacillus 30wt%, streptococcus acidi lactici 20wt% and Rhodopseudomonas palustris 20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
Embodiment 3
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 5: 95 by the composite bacteria that yeast saccharomyces cerevisiae 30wt%, plant lactobacillus 20wt%, streptococcus acidi lactici 30wt% and Rhodopseudomonas palustris 20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
Embodiment 4
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 5: 95 by the composite bacteria that yeast saccharomyces cerevisiae 30wt%, plant lactobacillus 30wt%, streptococcus acidi lactici 30wt% and Rhodopseudomonas palustris 10wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
It is as follows that the product that application the present invention makes carries out the deodorizing effect test:
After rubbish was transported to landfill yard, the reodorant that makes with the present invention sprayed processing, sprayed when sprinkling or rubbish were transported to landfill yard when mode was included in refuse collection.As both dual-purposes, effect is better, reodorant the main effect of landfill yard rubbish have following aspect:
Stench taste before 1, interior every rubbish of handling through sprinkling has not had;
2, descend in the continuous condition of no rain, the water from diffusion of rubbish is destitute of smell, and lifts up with hand to be placed on nose and to examine to smell and also have only slight stink or be destitute of smell at all.Through the test of environment monitoring center, this moment, COD reduced to 1000 from 8000;
3, worker's reaction in, After Hours on one's body, hair all is destitute of smell, and sensation in the course of the work is also more preceding comfortable many;
4, a limit passer was to seal nose with hand to pass through fast in the past, had loseed this kind phenomenon now.
Claims (1)
1. a method of utilizing complex microorganism to prepare reodorant is characterized in that, concrete steps are:
The first step: will be inoculated in the substratum by weight 1-10: 99-90 by the composite bacteria that yeast saccharomyces cerevisiae 25-30wt%, plant lactobacillus 20-30wt%, streptococcus acidi lactici 20-30wt% and Rhodopseudomonas palustris 10-20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, vitamin solution was sterilized 15 minutes at 110 ℃, composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution 0.8wt% that will be obtained by the first step, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed put into the inherent 30 ℃ of fermentations of closed tin and obtained reodorant in 15 days; Described trace element solution is made up of the water of ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus; Described vitamin solution is by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101964982A CN101671643B (en) | 2009-09-27 | 2009-09-27 | Method for preparing deodorizer by composite microorganism |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101964982A CN101671643B (en) | 2009-09-27 | 2009-09-27 | Method for preparing deodorizer by composite microorganism |
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| CN101671643A CN101671643A (en) | 2010-03-17 |
| CN101671643B true CN101671643B (en) | 2011-09-28 |
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| CN2009101964982A Expired - Fee Related CN101671643B (en) | 2009-09-27 | 2009-09-27 | Method for preparing deodorizer by composite microorganism |
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| CN102093968B (en) * | 2010-11-29 | 2012-09-19 | 中国农业科学院农业资源与农业区划研究所 | Compound microbial inoculum for deodorization |
| CN102813948A (en) * | 2011-06-07 | 2012-12-12 | 山西昌鑫生物农业科技有限公司 | Preparation method of composite biological deodorant |
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| CN111548960A (en) * | 2020-05-13 | 2020-08-18 | 深圳市东荣生物科技有限责任公司 | Compound microbial preparation for deodorization |
| CN114833180B (en) * | 2022-04-25 | 2023-02-03 | 深圳市同创环保科技有限公司 | Process for deodorizing household garbage, increasing methane yield and accelerating garbage degradation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1180849C (en) * | 2001-10-16 | 2004-12-22 | 广东省微生物研究所 | Microbial deodorant and its preparation method |
| CN101269227A (en) * | 2007-03-21 | 2008-09-24 | 北京世纪星伊埃姆生物技术研究所 | Biological agent for purifying air and preparation method thereof |
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2009
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1180849C (en) * | 2001-10-16 | 2004-12-22 | 广东省微生物研究所 | Microbial deodorant and its preparation method |
| CN101269227A (en) * | 2007-03-21 | 2008-09-24 | 北京世纪星伊埃姆生物技术研究所 | Biological agent for purifying air and preparation method thereof |
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