CN101671643B - Method for preparing deodorizer by composite microorganism - Google Patents
Method for preparing deodorizer by composite microorganism Download PDFInfo
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- CN101671643B CN101671643B CN2009101964982A CN200910196498A CN101671643B CN 101671643 B CN101671643 B CN 101671643B CN 2009101964982 A CN2009101964982 A CN 2009101964982A CN 200910196498 A CN200910196498 A CN 200910196498A CN 101671643 B CN101671643 B CN 101671643B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention provides a method for preparing a deodorizer by a composite microorganism, which is characterized by comprising the following concrete steps: (1) inoculating a composite strain comprising saccharomyces cerevisiae, lactobacillus plantarum, streptococcus acidi and rhodopseudomonas palustris into a culture medium, culturing for 48 hours at the temperature of 30 DEG C and obtaining composite microbial agent basic solution; (2) sterilizing sirup, sterilizing a vitamin solution, putting fermentiation basic solution composed of the composite microbial agent raw liquid, the sirup, a trace element solution, the vitamin solution and the balance water into a closed tank, fermenting and obtaining the deodorizer. The invention can quicken the fermentation and the decomposition of organicsubstances in rubbish and reduce the volume of the rubbish stack in a short time, thus increasing the quantity of the total rubbish stacking quantity.
Description
Technical field
The present invention relates to a kind of method of utilizing complex microorganism to prepare reodorant, belong to environmental protection technical field.
Background technology
China's domestic waste year amount of clearing is about 1.4 hundred million tons, and except small part burning, compost or recycling, all the other are transported to landfill yard and dispose more than 70%.Rubbish the depositing of landfill yard, load and unload, in the processes such as tiling, compacting, because wherein organic rotten decomposition unavoidably will produce odor pollution.Foul gas can be divided into 5 classes by its composition: 1. sulfocompound, and as H
2S, SO
2, mercaptan, thioether etc.; 2. nitrogenous compound is as ammonia, amine, phthalein amine, cry and make an uproar etc.; 3. halogen and derivative, as grab gas, halohydrocarbon etc.; 4. hydro carbons and aromatic hydrocarbon; 5. oxygen-bearing organic matter is as alcohol, phenol, aldehyde, ketone, organic acid etc.Stench both can the serious harm human health, will produce secondary pollution again.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing complex microorganism to prepare reodorant.
To achieve these goals, technical scheme of the present invention provides a kind of method of utilizing complex microorganism to prepare reodorant, it is characterized in that, concrete steps are:
The first step: will be inoculated in the substratum by weight 1-10: 99-90 by the composite bacteria that yeast saccharomyces cerevisiae 25-30wt%, plant lactobacillus 20-30wt%, streptococcus acidi lactici 20-30wt% and Rhodopseudomonas palustris 10-20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, vitamin solution was sterilized 15 minutes at 110 ℃, composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution 0.8wt% that will be obtained by the first step, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed put into the inherent 30 ℃ of fermentations of closed tin and obtained reodorant in 15 days.
Further, described trace element solution is made up of the water of ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus.
Described vitamin solution is by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed.
Yeast saccharomyces cerevisiae in the described the first step (Sacharomyces cerevisiae), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC20065.Plant lactobacillus (Lactobacillus plantarum), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC11118.Streptococcus acidi lactici (Streptococcus lactis), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC10653.Rhodopseudomonas palustris (Rhodopseudomonas palustris), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC10649.Above-mentioned four kinds of bacterial classifications all can be bought.
Utilize complex microorganism group's comprehensive action, the organism in rubbish and the percolate, noxious pollutant, foul smell etc. can effectively be decomposed, reach the effect of deodorizing and harmless processing.Photosynthetic bacterium wherein is with H
2The S reduction is decomposed into S and H
2Thereby, reach the de-odorised purpose; By the anaerobically fermenting of microorganism, the organic macromolecule in the percolate is decomposed into small molecules acid and dissolved matter, and microorganism by breeding, is oxidized to carbonic acid gas and water with it again, finishes biodegradation process.Lactobacillus in the composite flora can suppress the movable and breeding of pathogenic bacterium, sclerotium bacteria, and harmful line bacterium is faded away;
Advantage of the present invention is:
1, quickens organic fermentation decomposition in the rubbish, reduce the volume of piling up rubbish at short notice, thereby increase total scrap heap quantity high-volume.
2, suppress the growth of putrefactive bacterium, improve organic decomposition approach, reduce ammonia and the burst size of hydrogen sulfide and the generation of Ammonia material, the biogas amount that also reduces bacterial degradation simultaneously and produced, thus reach the de-odorised purpose.
3, suppress the pupa hatching of mosquito, the long-range effect of multiplying that reduces mosquitos and flies that reaches reduces the chance that causes disease.
4, suppress the breeding of pathogenic microbes, prevent the generation of disease.
5, reduce the generation of water from diffusion, and also improved the water quality of water from diffusion, reduce wherein each kind of pollutant component greatly.
6, promote landfill area soilization and regenerated process.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 10: 90 by the composite bacteria that yeast saccharomyces cerevisiae 25wt%, plant lactobacillus 30wt%, streptococcus acidi lactici 25wt% and Rhodopseudomonas palustris 20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
Embodiment 2
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 1: 99 by the composite bacteria that yeast saccharomyces cerevisiae 30wt%, plant lactobacillus 30wt%, streptococcus acidi lactici 20wt% and Rhodopseudomonas palustris 20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
Embodiment 3
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 5: 95 by the composite bacteria that yeast saccharomyces cerevisiae 30wt%, plant lactobacillus 20wt%, streptococcus acidi lactici 30wt% and Rhodopseudomonas palustris 20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
Embodiment 4
A kind of method of utilizing complex microorganism to prepare reodorant, concrete steps are:
The first step: will be inoculated in the substratum by weight 5: 95 by the composite bacteria that yeast saccharomyces cerevisiae 30wt%, plant lactobacillus 30wt%, streptococcus acidi lactici 30wt% and Rhodopseudomonas palustris 10wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, with vitamin solution (by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed) 110 ℃ of sterilizations 15 minutes, (water by ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus was formed for composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution that will be obtained by the first step.) 0.8wt%, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed is put into the inherent 30 ℃ of fermentations of closed tin and was obtained reodorant in 15 days.
It is as follows that the product that application the present invention makes carries out the deodorizing effect test:
After rubbish was transported to landfill yard, the reodorant that makes with the present invention sprayed processing, sprayed when sprinkling or rubbish were transported to landfill yard when mode was included in refuse collection.As both dual-purposes, effect is better, reodorant the main effect of landfill yard rubbish have following aspect:
Stench taste before 1, interior every rubbish of handling through sprinkling has not had;
2, descend in the continuous condition of no rain, the water from diffusion of rubbish is destitute of smell, and lifts up with hand to be placed on nose and to examine to smell and also have only slight stink or be destitute of smell at all.Through the test of environment monitoring center, this moment, COD reduced to 1000 from 8000;
3, worker's reaction in, After Hours on one's body, hair all is destitute of smell, and sensation in the course of the work is also more preceding comfortable many;
4, a limit passer was to seal nose with hand to pass through fast in the past, had loseed this kind phenomenon now.
Claims (1)
1. a method of utilizing complex microorganism to prepare reodorant is characterized in that, concrete steps are:
The first step: will be inoculated in the substratum by weight 1-10: 99-90 by the composite bacteria that yeast saccharomyces cerevisiae 25-30wt%, plant lactobacillus 20-30wt%, streptococcus acidi lactici 20-30wt% and Rhodopseudomonas palustris 10-20wt% form, cultivate at 30 ℃ and obtained composite fungus agent stoste in 48 hours;
Described substratum is made up of the water of peptone 0.8wt%, maltose 0.3wt%, glucose 1wt%, SODIUM PHOSPHATE, MONOBASIC 0.1wt% and surplus, and the pH value is 6.8;
Second the step: with molasses 121 ℃ the sterilization 20min, vitamin solution was sterilized 15 minutes at 110 ℃, composite fungus agent stoste 20-30wt%, molasses 25wt%, the trace element solution 0.8wt% that will be obtained by the first step, the fermenation raw liquid that the water of vitamin solution 0.2wt% and surplus is formed put into the inherent 30 ℃ of fermentations of closed tin and obtained reodorant in 15 days; Described trace element solution is made up of the water of ferrous sulfate 3.5wt%, sal epsom 0.75wt%, zinc sulfate 1.1wt% and surplus; Described vitamin solution is by vitamin H 2wt%, vitamins B
12The water of 12wt% and surplus is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009101964982A CN101671643B (en) | 2009-09-27 | 2009-09-27 | Method for preparing deodorizer by composite microorganism |
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| CN2009101964982A CN101671643B (en) | 2009-09-27 | 2009-09-27 | Method for preparing deodorizer by composite microorganism |
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| CN101671643A CN101671643A (en) | 2010-03-17 |
| CN101671643B true CN101671643B (en) | 2011-09-28 |
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| CN101885640B (en) * | 2010-06-25 | 2013-02-13 | 上海创博生态工程有限公司 | Method for preparing mushroom medium by using microbial composite bacteria |
| CN102093968B (en) * | 2010-11-29 | 2012-09-19 | 中国农业科学院农业资源与农业区划研究所 | Compound microbial inoculum for deodorization |
| CN102813948A (en) * | 2011-06-07 | 2012-12-12 | 山西昌鑫生物农业科技有限公司 | Preparation method of composite biological deodorant |
| CN102319444B (en) * | 2011-09-22 | 2015-10-07 | 澄思源生物科技（上海）有限公司 | A kind of microbe lavatory deodorant and preparation method thereof |
| CN103468619B (en) * | 2013-09-26 | 2019-03-26 | 青岛蔚蓝生物集团有限公司 | A kind of microorganism formulation purifying air |
| CN104689356A (en) * | 2013-12-05 | 2015-06-10 | 无锡市锡源锅炉有限公司 | Method for preparing drying furnace deodorant by composite microbes |
| CN104689357A (en) * | 2013-12-05 | 2015-06-10 | 无锡钻石地毯制造有限公司 | Method for preparing ecological carpet deodorant by composite microbes |
| CN103922482A (en) * | 2014-01-14 | 2014-07-16 | 厦门泰玮生物环保科技有限公司 | Deodorizing liquid based on compound microorganisms and growth promoters thereof |
| CN104353352B (en) * | 2014-11-04 | 2016-09-21 | 甘肃新德燃气有限公司 | A kind of vegetative microorganism liquid for deodorization and production method |
| CN105776563A (en) * | 2014-12-18 | 2016-07-20 | 江阴昊松格氏生物技术有限公司 | Preparation method of special biological deodorant used for sewage deodorization |
| CN105770953A (en) * | 2014-12-18 | 2016-07-20 | 江阴昊松格氏生物技术有限公司 | Preparation method of biological deodorant used for recycle bin deodorization |
| CN106731739A (en) * | 2015-11-23 | 2017-05-31 | 江阴昊松格氏生物技术有限公司 | Complex microorganism deodorant with Radix Codonopsis crape myrtle peppermint nostoc |
| CN106731728A (en) * | 2015-11-23 | 2017-05-31 | 江阴昊松格氏生物技术有限公司 | Complex microorganism deodorant with Radix Codonopsis crape myrtle peppermint nostoc |
| CN106186616A (en) * | 2016-09-07 | 2016-12-07 | 潍坊博士得生物科技有限公司 | A kind of ferment nutrient processing livestock and poultry feces toxin stench and preparation method thereof |
| CN106348556A (en) * | 2016-09-29 | 2017-01-25 | 张元生 | Latrine pit deodorant and preparation method thereof |
| CN106967640A (en) * | 2017-04-11 | 2017-07-21 | 上海莱泰生物科技有限公司 | A kind of preparation method of deodorizing microorganism microbial inoculum |
| CN108404647A (en) * | 2018-03-27 | 2018-08-17 | 湖南省蓝正微生物科技开发有限公司 | Complex microorganism deodorant |
| CN109364737A (en) * | 2018-12-27 | 2019-02-22 | 黄河三角洲京博化工研究院有限公司 | A kind of compound deodorizer |
| CN110591983A (en) * | 2019-10-30 | 2019-12-20 | 陕西天仁雪农业科技有限公司 | Biological deodorant based on compound microorganisms and preparation method thereof |
| CN111548960A (en) * | 2020-05-13 | 2020-08-18 | 深圳市东荣生物科技有限责任公司 | Compound microbial preparation for deodorization |
| CN114833180B (en) * | 2022-04-25 | 2023-02-03 | 深圳市同创环保科技有限公司 | Process for deodorizing household garbage, increasing methane yield and accelerating garbage degradation |
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| CN101671643A (en) | 2010-03-17 |
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