CN106676028A - FL-microbe mixing environment inocula and preparation method thereof - Google Patents
FL-microbe mixing environment inocula and preparation method thereof Download PDFInfo
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- CN106676028A CN106676028A CN201510760397.9A CN201510760397A CN106676028A CN 106676028 A CN106676028 A CN 106676028A CN 201510760397 A CN201510760397 A CN 201510760397A CN 106676028 A CN106676028 A CN 106676028A
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- fermentation
- bacterial classification
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Abstract
The invention relates to FL-microbe mixing environment inocula, which has good anaerobic bacteria species and facultative anaerobe species; the anaerobic bacteria species comprise yeast group series and gram-positive actinomyces series; the facultative anaerobe species comprise lactic acid bacteria group, photosynthetic bacteria, bifidobacteria, fermentation-series thread fungus group and bacillus spp. A preparation method of the FL-microbe mixing environment inocula comprises the following steps: a) preparing an aerobic fermentation medium; b) preparing a facultative anaerobic fermentation medium; and c) preparing an anaerobic fermentation finished product. According to the invention, comprehensive effect and synergism of the composite microbe are used, the main stink sources such as ammonia and hydrogen sulfide in house refuse landfill are effectively decomposed and absorbed, and the effects for deodorizing and purifying air are achieved.
Description
Technical field
The present invention relates to a kind of FL- microorganisms hybird environment microbial inoculum and preparation method thereof.
Background technology
With the rapid raising of the development of social economy, the quickening of urbanization process and living standards of the people,
Urbanization production increases sharply with the junk of generation in life process, house refuse land occupation landfill,
The pernicious stink of its release causes atmosphere pollution, has a strong impact on the health of surrounding enviroment and the people.
The stink main method removed in rubbish has：Bioanalysis, absorption method and combustion method.It is relatively more normal at present
It is bioanalysis with method.Biological deodorization technology is a kind of by adding the screening from nature in environment
Beneficial microbe colony or the biological inoculum produced through genetic recombination, to improve air ambient, effectively suppress
Mosquito is grown for purpose Environmental Biotechnology.
The content of the invention
The technical problem to be solved in the present invention is：In view of microorganism energy effective process consumer waste filling and embedding is pernicious
The broad prospect of application of stink, the present invention provides a kind of FL- microorganisms hybird environment microbial inoculum and preparation method thereof,
It includes various bacterial strains, and rationally, synergy is strong for compatibility, effectively can remove the same of garbage odor by fast decoupled
When suppress various peculiar smell to generate.
The technical solution adopted for the present invention to solve the technical problems is：A kind of FL- microorganisms hybird environment bacterium
Agent, with aerobic bacterial classification and amphimicrobian bacterial classification；Described aerobic bacterial classification includes yeast flora and gram sun
Property actinomycete group；Described amphimicrobian bacterial classification includes lactobacillus, photosynthetic bacteria, Bifidobacterium, fermentation system
Thread flora and bacillus.
A kind of method for preparing FL- microorganism hybird environment microbial inoculums, comprises the following steps that shown：
A, prepare aerobic fermentation culture medium：Aerobic bacterial classification is inoculated into fluid nutrient medium mixing to be fitted into fermentation tank,
Mix 28～32 minutes, uniformly supply from fermenter base, make zymotic fluid slowly circulate, temperature keeps
At 28～32 DEG C, fermentation time is 46～50 hours, and viable count is no less than 107cfu/ml；
B, prepare amphimicrobian fermentation medium：Amphimicrobian bacterial classification is inoculated into fluid nutrient medium mixing to load
In fermentation tank, mix 28～32 minutes, keep the temperature at 28～32 DEG C, sealing and fermenting 46～50
Hour, viable count is no less than 107cfu/ml；
C, prepare anaerobic fermentation finished product：Fluid nutrient medium is fitted into fermentation tank, its pol is adjusted to 8%～12%,
Add sodium bicarbonate(edible) and adjust pH value to 7.1～7.5, begin to warm up after stirring, temperature control is existed
88～92 DEG C, maintain 28～32 minutes, less than 33～37 DEG C are reduced the temperature to after sterilizing, in adding step a
The aerobic fermentation culture medium for obtaining and the amphimicrobian fermentation medium obtained in step b, add lactic acid bacteria
Kind, mixing 28～32 minutes, temperature is maintained at 28～32 DEG C, anaerobic fermentation 6～8 days, and pH value is
3.6～4.0, total viable count is no less than 107Cfu/ml, obtains final product anaerobic fermentation finished product
The invention has the beneficial effects as follows：Comprehensive function and synergy using complex microorganism, to life rubbish
The main source of odor such as ammonia and hydrogen sulfide in rubbish landfill carries out effectively decomposing and absorbing, and reaches deodorant purify air
Effect.
Specific embodiment
A kind of FL- microorganisms hybird environment microbial inoculum of the present invention, with aerobic bacterial classification and amphimicrobian bacterial classification；It is aerobic
Bacterial classification includes yeast flora and Gram positive actinomycetes group；Amphimicrobian bacterial classification includes lactobacillus, photosynthetic
Bacterium, Bifidobacterium, the thread flora of fermentation system and bacillus.
Hydrogen sulfide is resolved into sulphur and hydrogen by photosynthetic bacteria, so as to reach deodorant purpose, and detesting by microorganism
Aerobe fermentation, by some organic macromolecules be decomposed into small molecule acid and dissolved matter, microorganism by breeding,
It is oxidized as carbon dioxide and water, completes biodegradable process.
Lactobacillus (containing Bifidobacterium) form lactic acid by absorbing the carbohydrate that photosynthetic bacteria, yeast flora are produced, and have
Effect suppresses the activity and breeding of germ.
Gram positive actinomycetes group obtains amino acid, nitrogen etc. as matrix from photosynthetic bacterial classification, and output is various
Antibiotics, vitamin and enzyme, can directly suppress pathogen.
The der Pilz group energy of fermentation system and other microorganisms coexist, because alcohol producing power is strong, can prevent and treat rubbish
The generation of middle maggot and other insects, it is possible to eliminate stench.
Bacillus organic matter decomposition power is strong, while propagation, can disengage highly active catabolic enzyme, will decompose
Macromolecular substances resolve into available small-molecule substance, suppress harmful levels of pathogens growth, harmful power of going out strong.
Embodiment one：
The method that a kind of the invention prepares FL- microorganism hybird environment microbial inoculums, comprises the following steps that shown：
A, prepare aerobic fermentation culture medium：Aerobic bacterial classification is inoculated into fluid nutrient medium mixing to be fitted into fermentation tank,
Mix 28 minutes, uniformly supply from fermenter base, make zymotic fluid slowly circulate, temperature is maintained at 28 DEG C,
Fermentation time is 46 hours, and viable count is no less than 107cfu/ml；
B, prepare amphimicrobian fermentation medium：Amphimicrobian bacterial classification is inoculated into fluid nutrient medium mixing to load
In fermentation tank, mix 28 minutes, keep the temperature at 28 DEG C, sealing and fermenting 46 hours, viable count
It is no less than 107cfu/ml；
C, prepare anaerobic fermentation finished product：Fluid nutrient medium is fitted into fermentation tank, its pol is adjusted to 8%, then
Add sodium bicarbonate(edible) to adjust pH value to 7.1, begin to warm up after stirring, by temperature control at 88 DEG C,
Maintain 28 minutes, less than 33 DEG C are reduced the temperature to after sterilizing, add the aerobic fermentation culture obtained in step a
The amphimicrobian fermentation medium obtained in base and step b, adds lactobacillus inoculation, mixes 28 minutes,
Temperature is maintained at 28 DEG C, anaerobic fermentation 6 days, and pH value is 3.6, and total viable count is no less than 107Cfu/ml,
Obtain final product anaerobic fermentation finished product.
Embodiment two：
The method that a kind of the invention prepares FL- microorganism hybird environment microbial inoculums, comprises the following steps that shown：
A, prepare aerobic fermentation culture medium：Aerobic bacterial classification is inoculated into fluid nutrient medium mixing to be fitted into fermentation tank,
Mix 30 minutes, uniformly supply from fermenter base, make zymotic fluid slowly circulate, temperature is maintained at 30 DEG C,
Fermentation time is 48 hours, and viable count is no less than 107cfu/ml；
B, prepare amphimicrobian fermentation medium：Amphimicrobian bacterial classification is inoculated into fluid nutrient medium mixing to load
In fermentation tank, mix 30 minutes, keep the temperature at 30 DEG C, sealing and fermenting 48 hours, viable count
It is no less than 107cfu/ml；
C, prepare anaerobic fermentation finished product：Fluid nutrient medium is fitted into fermentation tank, its pol is adjusted to 10%,
Add sodium bicarbonate(edible) and adjust pH value to 7.3, begin to warm up after stirring, by temperature control at 90 DEG C,
Maintain 30 minutes, less than 35 DEG C are reduced the temperature to after sterilizing, add the aerobic fermentation culture obtained in step a
The amphimicrobian fermentation medium obtained in base and step b, adds lactobacillus inoculation, mixes 30 minutes,
Temperature is maintained at 30 DEG C, anaerobic fermentation 7 days, and pH value is 3.8, and total viable count is no less than 107Cfu/ml,
Obtain final product anaerobic fermentation finished product.
Embodiment three：
The method that a kind of the invention prepares FL- microorganism hybird environment microbial inoculums, comprises the following steps that shown：
A, prepare aerobic fermentation culture medium：Aerobic bacterial classification is inoculated into fluid nutrient medium mixing to be fitted into fermentation tank,
Mix 32 minutes, uniformly supply from fermenter base, make zymotic fluid slowly circulate, temperature is maintained at 32 DEG C,
Fermentation time is 50 hours, and viable count is no less than 107cfu/ml；
B, prepare amphimicrobian fermentation medium：Amphimicrobian bacterial classification is inoculated into fluid nutrient medium mixing to load
In fermentation tank, mix 32 minutes, keep the temperature at 32 DEG C, sealing and fermenting 50 hours, viable count
It is no less than 107cfu/ml；
C, prepare anaerobic fermentation finished product：Fluid nutrient medium is fitted into fermentation tank, its pol is adjusted to 12%,
Add sodium bicarbonate(edible) and adjust pH value to 7.5, begin to warm up after stirring, by temperature control at 92 DEG C,
Maintain 32 minutes, less than 37 DEG C are reduced the temperature to after sterilizing, add the aerobic fermentation culture obtained in step a
The amphimicrobian fermentation medium obtained in base and step b, adds lactobacillus inoculation, mixes 32 minutes,
Temperature is maintained at 32 DEG C, anaerobic fermentation 8 days, and pH value is 4.0, and total viable count is no less than 107Cfu/ml,
Obtain final product anaerobic fermentation finished product.
With the above-mentioned desirable embodiment according to the present invention as enlightenment, by above-mentioned description, related work
Personnel can carry out various change and modification in the range of without departing from this invention technological thought completely.
The technical scope of this invention is not limited to the content on specification, it is necessary to according to right
To determine its technical scope.
Claims (2)
1. a kind of FL- microorganisms hybird environment microbial inoculum, it is characterised in that：With aerobic bacterial classification and amphimicrobian
Bacterial classification；Described aerobic bacterial classification includes yeast flora and Gram positive actinomycetes group；Described amphimicrobian
Bacterial classification includes lactobacillus, photosynthetic bacteria, Bifidobacterium, the thread flora of fermentation system and bacillus.
2. a kind of method for preparing FL- microorganisms hybird environment microbial inoculum as claimed in claim 1, its feature
It is：Shown in comprising the following steps that：
A, prepare aerobic fermentation culture medium：Aerobic bacterial classification is inoculated into fluid nutrient medium mixing to be fitted into fermentation tank,
Mix 28～32 minutes, uniformly supply from fermenter base, make zymotic fluid slowly circulate, temperature keeps
At 28～32 DEG C, fermentation time is 46～50 hours, and viable count is no less than 107cfu/ml；
B, prepare amphimicrobian fermentation medium：Amphimicrobian bacterial classification is inoculated into fluid nutrient medium mixing to load
In fermentation tank, mix 28～32 minutes, keep the temperature at 28～32 DEG C, sealing and fermenting 46～50
Hour, viable count is no less than 107cfu/ml；
C, prepare anaerobic fermentation finished product：Fluid nutrient medium is fitted into fermentation tank, its pol is adjusted to 8%～12%,
Add sodium bicarbonate(edible) and adjust pH value to 7.1～7.5, begin to warm up after stirring, temperature control is existed
88～92 DEG C, maintain 28～32 minutes, less than 33～37 DEG C are reduced the temperature to after sterilizing, in adding step a
The aerobic fermentation culture medium for obtaining and the amphimicrobian fermentation medium obtained in step b, add lactic acid bacteria
Kind, mixing 28～32 minutes, temperature is maintained at 28～32 DEG C, anaerobic fermentation 6～8 days, and pH value is
3.6～4.0, total viable count is no less than 107Cfu/ml, obtains final product anaerobic fermentation finished product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107789372A (en) * | 2017-11-30 | 2018-03-13 | 合肥海昭生物科技有限公司 | A kind of preparation method of microbial metabolism organic acid antibacterial liquid |
| CN108660096A (en) * | 2018-05-22 | 2018-10-16 | 浙江省桐庐汇丰生物科技有限公司 | A kind of cultural method of amphimicrobian bacillus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591631A (en) * | 2009-06-08 | 2009-12-02 | 江西省天意生物技术开发有限公司 | EM original solution and production method thereof |
| CN101779608A (en) * | 2010-02-04 | 2010-07-21 | 刘英宁 | Method for manufacturing livestock cultivation fermentation bed and swine production method |
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2015
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591631A (en) * | 2009-06-08 | 2009-12-02 | 江西省天意生物技术开发有限公司 | EM original solution and production method thereof |
| CN101779608A (en) * | 2010-02-04 | 2010-07-21 | 刘英宁 | Method for manufacturing livestock cultivation fermentation bed and swine production method |
Non-Patent Citations (2)
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| 中青农村发展中心主编: "《全国农村百项致富技术项目：精选版》", 31 July 1998, 中国经济出版社 * |
| 梁利宝编著: "《微生物肥料与农药在现代农业生产中的应用》", 31 July 2012, 中国农业科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107789372A (en) * | 2017-11-30 | 2018-03-13 | 合肥海昭生物科技有限公司 | A kind of preparation method of microbial metabolism organic acid antibacterial liquid |
| CN108660096A (en) * | 2018-05-22 | 2018-10-16 | 浙江省桐庐汇丰生物科技有限公司 | A kind of cultural method of amphimicrobian bacillus |
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