CN106520627A - Microbial sludge deodorant and preparation method thereof - Google Patents
Microbial sludge deodorant and preparation method thereof Download PDFInfo
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- CN106520627A CN106520627A CN201611118083.XA CN201611118083A CN106520627A CN 106520627 A CN106520627 A CN 106520627A CN 201611118083 A CN201611118083 A CN 201611118083A CN 106520627 A CN106520627 A CN 106520627A
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- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000020265 peanut milk Nutrition 0.000 description 1
- 230000003567 photophosphorylation Effects 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical class NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 108010082567 subtilin Proteins 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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Abstract
The invention discloses a microbial sludge deodorant and a preparation method thereof, wherein the preparation method comprises the following steps of respectively carrying out high-density culture on strains of streptococcus thermophilus, bacillus subtilis, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and pseudomonas putida, dehydrating and drying various single bacteria subjected to high-density culture to prepare dormant microbe dry powder, and then mixing according to the weight ratio of 10-20 parts of streptococcus thermophilus, 10-15 parts of bacillus subtilis, 20-30 parts of aspergillus oryzae, 10-15 parts of photosynthetic bacteria, 10-20 parts of saccharomyces cerevisiae, 10-15 parts of lactobacillus casei and 10-20 parts of pseudomonas putida to obtain the microbial sludge deodorant. The invention is used for deodorizing garbage, and has the advantages of small production investment, low production cost, convenient use and good deodorizing effect compared with the prior art.
Description
Technical field
The present invention relates to a kind of preparation method, and in particular to a kind of microorganism sludge deodorizer and preparation method thereof, belong to
Environmental protection technical field.
Background technology
In recent years, the raising with people to environmental protection understanding, sewage disposal have obtained fast development, but band therewith in China
Carry out another serious environmental issue, here it is the sludge produced after how properly disposing sewage disposal, it has become whole sewage
Treatment industry problem demanding prompt solution.The sludge handled well will be a kind of good resource, but random stand is put and must be caused to ring
The secondary pollution in border.The best way for processing sludge at present is exactly to comprehensively utilize, will sludge addition inorganic fertilizer, life
Calx or flyash, Jing are simply processed for agricultural production.This method is both economical, and meet that China advocated now can
After the strategy of sustainable development, but the foundation with some municipal sludge treatment plants and actual motion, it is found that the method there is also one
A little problems, in one of subject matter generating process, sludge gives an offensive smell.The reason for producing stink is because at municipal sludge
The sludge of reason factory contains part band material frowzy, such as：Sulfide, ammonia, putrescine class etc., so when by its course of processing
When, can be to surrounding emitting offensive odor, major polluting atmosphere environment.
At present, common deodorizing method has chemical method and a Physical, but its use various equipment, complex process, energy consumption it is big,
Operating cost is high, and deodorizing effect is not so good, and the basicity for not being the increase in sludge is just the increase in the volume of sludge.Microorganism is removed
Smelly technology is that will have material frowzy by the physiological metabolism of microorganism to be converted, and reaches the purpose to sludge deodorization.With
Other deodorizing methods are compared, and microbial deodorant method has the advantages that small investment, effect be good, maintenance management is simple and convenient to operate.Examine
Considering domestic many middle or small sewage treatments does not have sludge digestion operation, and generation is primary sludge, using the side of biological deodorizing
Method can play a part of sludge digestion operation, so preferable using microbial deodorant method.Now, domestic and international market exists many raw
Thing deodorising product, but it is less for the product of the biological deodorant of municipal sludge.Have even with single bacillus subtilises
Biological deodorant is served as in agent, though complicated components also, it is unstable to there is effective flora ratio, causes product quality and use
In effect, problem is continuous.
The content of the invention
The present invention is exactly for technical problem present in prior art, there is provided a kind of efficient, pollution-free removal sludge
Microbial deodorant of middle abnormal flavour and preparation method thereof, the method adopt High Density Cultivation mode, reduce fermentation costs, reduce
The generation of miscellaneous bacteria, stabilizes product quality, can reduce the discharge of harmful gass during comprehensive utilization of mud, purifies production site
Environment, mitigate atmospheric pollution.
To achieve these goals, technical scheme is as follows, a kind of microorganism sludge deodorizer, microorganism sludge
Deodorizer, by streptococcus thermophiluss (Streptococcus thermophilus), bacillus subtilises（Bacillus subtilis）, aspergillus oryzae（Aspergillus oryzae）, photosynthetic bacteria（photosynthetic bacteria）, ferment of making wine
It is female（Saccharomyces cerevisiae）, lactobacillus casei（Lactobacillus casei）And pseudomonas putida
(Pseudomonas putida) is constituted, wherein：10～20 parts of streptococcus thermophiluss, 10～15 parts of bacillus subtilises, aspergillus oryzae
20～30 parts, 10～15 parts of photosynthetic bacteria, 10～20 parts of saccharomyces cerevisiae, 10～15 parts of lactobacillus casei and pseudomonas putida 10
～20 parts；
The preferred streptococcus thermophiluss CGMCC1.3996 of each component, bacillus subtilises in mentioned microorganism Sludge deodorant
CGMCC1.1160, aspergillus oryzae CGMCC3.4437, photosynthetic bacteria CGMCC1.7520, saccharomyces cerevisiae CGMCC2.1793, cheese breast
Bacillus CGMCC1.3113 and pseudomonas putida CGMCC1.2309；Above strain is bought from China General Microbiological strain and is protected
Administrative center is hidden, the preservation time is in October, 2015.The technical scheme passes through separation screening efficient deodorizing function stem and optimizes
Combination and compatibility to its foul gass can carry out decomposition utilization using the microbial inoculum into microbial deoderizer, so as to reduce sludge money
During sourceization utilization, the discharge of harmful gass, reaches purification surrounding, reduces the purpose of atmospheric pollution.
A kind of preparation method of microorganism sludge deodorizer, it is characterised in that methods described is as follows, 1）By thermophilus
The strain of bacterium, bacillus subtilises, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and pseudomonas putida carries out height
Density culture；2）Above-mentioned strain is seeded to into activation culture in the shaking flask equipped with the culture medium that volume ratio is 15～30% respectively, will
Cultured strain is inoculated in fermentation tank carries out fermentation amplification culture, will be enlarged by culture and obtains various single bacterium dehydrate, system
For into hypopuss microbial dry powder；3）By step 2）In mycopowder according to 10～20 parts of streptococcus thermophiluss, bacillus subtilises 10
～15 parts, 20～30 parts of aspergillus oryzae, 10～15 parts of photosynthetic bacteria, 10～20 parts of saccharomyces cerevisiae, 10～15 parts of lactobacillus casei and
The weight ratio that 10～20 parts of pseudomonas putida, is mixed to get microorganism sludge deodorizer.
As a modification of the present invention, step 2）The preparation of middle streptococcus thermophiluss hypopuss microbial dry powder includes following
Step：Thermophilus strain on the slant medium of purification culture is seeded to equipped with the activation training that volume ratio is 15～30%
In the shaking flask of foster base, the activation culture based formulas are 6.5～8.0g/l of yeast extract, 6.5～8.0g/l of peptone, glucose 8
～12g/l, 1～3g/l of dipotassium hydrogen phosphate, 50～200ml of Tomato juice, pH value 6.3～7.2, temperature are 46～50 DEG C, shaking flask
120～160rpm of rotating speed, 30～42h of activation culture；
Activation culture good thermophilus strain is inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium,
Inoculum concentration is volume ratio 1～3%, and the formula of the fermentation medium is 5～6g/l of corn cob, 8～12g/l of glucose, yeast extract
8～12g/l, 18～22g/l of potassium dihydrogen phosphate, 0.1～0.5g/l of magnesium sulfate, 0.2～0.6g/l of calcium chloride, in cultivation temperature be
48～50 DEG C, pH value 6.3～7.2, fermentation tank 120～160rpm of speed of agitator, 60～72h of amplification culture are thermophilic into fermentation tank
Hot hammer bacterial content >=108Individual/ml, then to be prepared into hypopuss micro- by the streptococcus thermophiluss list bacterium that obtains of fermentation freeze-dried
Biological dry powder.
As a modification of the present invention, step 2）The preparation of the hypopuss microbial dry powder of middle bacillus subtilises includes
Following steps：It is 15～30% that Bacillus subtilis strain on the slant medium of purification culture is seeded to equipped with volume ratio
Activation medium shaking flask in, the formula of the activation medium be 0.2～0.8g/l of Carnis Bovis seu Bubali cream, 12～18g/l of peptone,
3～7g/l of 18～22g/l of glucose and Sodium Chloride, is 36～40 DEG C in cultivation temperature, pH value 6.5～7.5, shaking flask rotating speed 150
～190rpm cultivates 16～24h；It is 50～70% that the good Bacillus subtilis strain of activation culture is inoculated into equipped with volume ratio
In the fermentation tank of ferment culture medium, volume inoculum concentration is 1～3%, and the formula of the fermentation medium is 18～22g/l of soybean cake powder, Portugal
Grape 3～8g/l of sugar, 3～8g/l of fish flour, 10～15g/l of Semen Maydis powder, 0.1～0.5g/l of dipotassium hydrogen phosphate, ammonium sulfate 0.8～
1.2g/l, 0.1～0.3g/l of manganese sulfate, 0.1～0.3g/l of 5～9g/l of Calcium Carbonate and magnesium sulfate, are 36～40 in cultivation temperature
DEG C, pH value 6.5～7.5,150～190rpm of fermentation tank speed of agitator cultivate 22～34h, bacillus subtilis bacterial content in fermentation tank
≥109Individual/ml, then bacterium is freeze-dried is prepared into hypopuss microbial dry powder by the bacillus subtilises that obtain of fermentation single.
As a modification of the present invention, step 2）The preparation of the hypopuss microbial dry powder of middle aspergillus oryzae includes following step
Suddenly：
Aspergillus oryzae strain on the slant medium of purification culture is seeded to equipped with the activation medium that volume ratio is 15～30%
Shaking flask in, the formula of the activation medium be 1～5g/l of glucose, 18～22g/l of potato juice, 1～2g/l of magnesium sulfate,
1～5g/l of dipotassium hydrogen phosphate, 0.01～0.05g/l of thiamine, are 28～30 DEG C in cultivation temperature, pH value 5.5～6.5, shaking flask
120～160rpm of rotating speed cultivates 28～36h；It is 50～70% that activation culture good aspergillus oryzae strain is inoculated into equipped with volume ratio
In the fermentation tank of fermentation medium, volume inoculum concentration is 1～3%, and the formula of the fermentation medium is 20～40g/l of sucrose, jade
25～35g/l of Rice ＆ peanut milk, 4～8g/l of carbamide, 0.3～0.8g/l of magnesium sulfate, 0.3～0.8g/l of potassium chloride, dipotassium hydrogen phosphate 0.8～
8～12g/l of 1.2g/l and olive oil, is 28～30 DEG C in cultivation temperature, pH value 5.5～6.5, fermentation tank speed of agitator 120～
160rpm, cultivates 36～50h, aspergillus oryzae content >=10 in fermentation tank8Individual/ml, the aspergillus oryzae list bacterium Jing that then fermentation is obtained
Lyophilization is prepared into hypopuss microbial dry powder.
As a modification of the present invention, step 2）The preparation of the hypopuss microbial dry powder of middle photosynthetic bacteria includes following
Step：Photosynthetic bacteria strain on the slant medium of purification culture is seeded to equipped with the culture medium that volume ratio is 15～30%
Shaking flask in, cultivation temperature be 28～30 DEG C, pH value 7.0～7.5, shaking flask every 24 hours stirring once, culture 64～
84h；Activation culture good photosynthetic bacteria strain is inoculated into equipped with the fermentation tank that volume ratio is 50～70% culture medium, volume
Inoculum concentration is 6～10%, is 28～30 DEG C in cultivation temperature, and pH value 7.0～7.5 was stirred half an hour every 24 hours, and stirring turns
140～180rpm of speed, cultivates 100～150h, photosynthetic bacteria content >=10 into fermentation tank8Then fermentation is obtained by individual/ml
Photosynthetic bacteria list bacterium is freeze-dried to be prepared into hypopuss microbial dry powder, and the activation culture is matched somebody with somebody with culture medium when fermenting
Side is 0.1～0.3g/l of dipotassium hydrogen phosphate, 1～3g/l of ammonium chloride, 0.5～1.5g/l of Sodium Chloride, 2～6g/l of sodium acetate, carbonic acid
Hydrogen 1～3g/l of sodium, 0.1～0.2g/l of 0.1～0.3g/l of magnesium sulfate and yeast extract.
As a modification of the present invention, step 2）The preparation of middle saccharomyces cerevisiae hypopuss microbial dry powder includes following step
Suddenly：
Saccharomyces cerevisiae strain on the slant medium of purification culture is seeded to equipped with the culture medium that volume ratio is 15～30%
In shaking flask, cultivation temperature be 28～30 DEG C, pH value 6.2～6.8, shaking flask 140～180rpm of rotating speed, 32～48h of activation culture；
Activation culture good saccharomyces cerevisiae strain is inoculated into equipped with the fermentation tank that volume ratio is 50～70% culture medium, inoculum concentration is
Culture medium when volume ratio 1～3%, the activation culture and fermentation is beerwort potato sucrose culture medium, in cultivation temperature is
28～30 DEG C, pH value 6.2～6.8, fermentation tank 140～180rpm of speed of agitator, 68～84h of amplification culture are made into fermentation tank
Brewer yeast content >=109Individual/ml, the saccharomyces cerevisiae list bacterium for then obtaining fermentation is freeze-dried to be prepared into hypopuss microorganism
Dry powder.
As a modification of the present invention, step 2）The preparation of the hypopuss microbial dry powder of middle lactobacillus casei include with
Lower step：Species L. casei on the slant medium of purification culture is seeded to equipped with the work that volume ratio is 15～30%
Change in the shaking flask of culture medium, the formula of the activation medium is 1～5g/l of Carnis Bovis seu Bubali cream, 8～12g/l of peptone and Sodium Chloride 3
～7g/l, is 36～40 DEG C in cultivation temperature, and pH value 6.8～7.2, shaking flask 180～220rpm of rotating speed cultivate 20～32h；
The good species L. casei of activation culture is inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium
Interior, volume inoculum concentration is 1～3%, and the formula of the fermentation medium is 13～17g/l of Semen Maydis powder, 13～17g/l of soybean cake powder, phosphorus
Acid dihydride 0.05～0.15g/l of potassium, 0.05～0.1g/l of manganese sulfate, 0.1～0.2g/l of 15～20g/l of Calcium Carbonate and magnesium sulfate,
It it is 36～40 DEG C in cultivation temperature, pH value 6.8～7.2,180～220rpm of fermentation tank speed of agitator cultivate 20～32h, fermentation tank
Middle lactobacillus casei content >=109Then the lactobacillus casei list bacterium drying that fermentation is obtained is prepared into hypopuss micro- by individual/ml
Biological dry powder.
As a modification of the present invention, step 2）The preparation of the hypopuss microbial dry powder of middle pseudomonas putida includes
Following steps：It is 15～30% that Pseudomonas putida species on the slant medium of purification culture are seeded to equipped with volume ratio
Activation medium shaking flask in, the formula of the activation medium is 1～5g/l of Carnis Bovis seu Bubali cream, 8～12g/l of peptone and chlorination
3～7g/l of sodium, is 26～30 DEG C in cultivation temperature, and pH value 7.0～7.5, shaking flask 120～160rpm of rotating speed cultivate 18～26h；
The good Pseudomonas putida species of activation culture are inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium
Interior, volume inoculum concentration is 1～3%, and the formula of the fermentation medium is 3～8g/l of glucose, 10～15g/l of Semen Maydis powder, fish flour
3～8g/l, 18～22g/l of soybean cake powder, 0.8～1.2g/l of ammonium sulfate, 5～9g/l of Calcium Carbonate, 0.1～0.3g/l of magnesium sulfate, sulfur
Sour 0.1～0.3g/l of manganese and 0.1～0.5g/l of dipotassium hydrogen phosphate, is 26～30 DEG C in cultivation temperature, pH value 7.0～7.5, fermentation
180～200rpm of tank speed of agitator cultivates 24～32h, pseudomonas putida content >=10 in fermentation tank9Individual/ml, then will send out
The pseudomonas putida list bacterium drying that ferment is obtained is prepared into hypopuss microbial dry powder.
Microorganism sludge deodorizer is used for municipal sludge deodorization.
Relative to prior art, the invention has the advantages that, 1）Streptococcus thermophiluss in the technical scheme enter to sludge
Row fermentation process, can improve response speed, eliminate the pathogenic microorganism in sludge, in fermentation industry, it is possible to use its resistance to height
The characteristic of temperature, improves reaction temperature, increases response speed, reduces the chance of warm type living contaminants in minimizing.Streptococcus thermophiluss can be produced
Various enzymes, such as cellulase, protease, amylase, lipase, dextrase etc., by the enzyme preparation produced in these microorganisms
Heat stability is good, rate of catalysis reaction is high, it is easy to preserve at room temperature；Bacillus subtilises in the technical scheme are alphalise starch
The important production bacterium of enzyme and neutral protease, the subtilin produced during bacillus subtilises thalli growth, polymyxin,
Nystatin, Gramicidin isoreactivity material, have obvious inhibitory action to the conditioned pathogen of pathogenic bacterium or autogenous infection；
The enzymes such as bacillus subtilises thalline itself synthesis α-amylase, protease, lipase, cellulase；Bacillus subtilises pair
The noxious bacteria such as vibrio, escherichia coli and baculoviruss have very strong inhibitory action, secrete the function of a large amount of chitinases, chitin
The cell wall of matter enzyme decomposable asymmetric choice net pathogenic fungi and suppress fungal disease, decompose poisonous and harmful substance in sludge, with very strong clear
The effect of reason sludge small particles；Aspergillus oryzae in the program is the bacterial strain that a class produces compound enzyme, in addition to protease is produced, can also be produced
Amylase, saccharifying enzyme, cellulase, phytase etc..In the presence of amylase, the straight chain in raw material, amylopectin are degraded
For dextrin and various low molecule saccharides, such as maltose, glucose etc.；In the presence of protease, by stodgy macromole
Protein degradation is peptone, polypeptide and various aminoacid, is widely used in the fermentation works such as sludge fermentation, production kojic acid, wine brewing
Industry；Photosynthetic bacteria in the program is using light as the energy, can utilize in nature under anaerobism illumination or aerobic dark condition
Organic substance, sulfide, ammonia etc. carry out photosynthetic microorganism as hydrogen donor and carbon source.Lead to nature is light, in sea water
Often per milliliter contains more or less a hundred PSB bacterium, and the thalline of photosynthetic bacteria is with the Organic substances such as organic acid, aminoacid, ammonia and carbohydrate and sulfuration
Hydrogen obtains energy by photophosphorylation as oxygen donator, can directly using degraded organic matter and hydrogen sulfide under illumination condition
And make itself to be bred, it is same to enter to have purified air；Saccharomyces cerevisiae in the program can decompose using the saccharide in environment, sulfuration
Hydrogen, ammonia etc., produce acidic materials in metabolic engineering, can effectively suppress the bacteria growing that causes a disease, and yeast thalline can be used as other
Coordinate the nutrient substance of bacterium, be conducive to the fast breeding of composite flora；Lactobacillus casei in the program can suppress and kill
Many putrefaction bacteria and pathogenic bacterium in sludge, and sludge character is not affected, or even food properties can be improved, therefore added
Become apparent from can product deodorizing effect in being added to deodorizer, positive role is played in the discharge to reducing odorous gas；The program
In pseudomonas putida with phenol as carbon source and the energy, phenol type substances in sludge of effectively can degrading reduce poisonous and harmful
The discharge of gas.While many putrefaction bacteria and pathogenic bacteria growing in can suppressing sludge, reduce the generation of odorous gas；2）This
Invention is by streptococcus thermophiluss, bacillus subtilises, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and Pseudomonas putida
The hypopuss microbial dry powder that the strain of bacterium is prepared into after carrying out High Density Cultivation, adopts rational proportion according to its purposes, mixing
Microorganism sludge deodorizer is obtained, is had greatly improved than the deodorizing effect of single antibacterial；3）The program utilizes composite microbial
The synergism of thing group, up to 100,000,000 per gram of bacterium number in deodorizer, organic principle that can rapidly in sludge, to the ammonia in sludge
The odor source main with hydrogen sulfide etc. carries out effectively decomposing and absorbing, and fundamentally reduces foul smell source of generation, reaches deodorization
Effect；4）The program is used for sludge deodorization, and compared to existing technology, investment of production is little, and low production cost is easy to use, and
Good deodorization effect, stink can be substantially eliminated within 3-4 days, and the Sludge deodorant has environment-friendly type, degradation capability under strain room temperature used
By force, can perish of oneself after pollutant are broken off, be decomposed into carbon dioxide, water and nontoxic cell residuum, degree of scatter is high,
It is nontoxic to humans and animals, secondary pollution will not be caused.
Specific embodiment：
In order to deepen the understanding of the present invention, the technical program is described in detail with reference to embodiment.
Embodiment 1：
It is respectively that streptococcus thermophiluss, bacillus subtilises, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and stench is false
The strain of Zymomonas mobiliss carries out High Density Cultivation：
（1）Streptococcus thermophiluss：It is 15% that thermophilus strain on the slant medium of purification culture is seeded to equipped with volume ratio
Activation medium shaking flask in, the activation culture based formulas be yeast extract 6.5g/l, peptone 6.5g/l, glucose 8g/
L, dipotassium hydrogen phosphate 1g/l, Tomato juice 50ml, pH value 6.5, temperature be 50 DEG C, shaking flask rotating speed 160rpm, activation culture 42h；
Activation culture good thermophilus strain is inoculated into equipped with the fermentation tank that volume ratio is 50% fermentation medium, inoculum concentration is
Volume ratio 1%, the formula of the fermentation medium is corn cob 5g/l, glucose 8g/l, yeast extract 8g/l, potassium dihydrogen phosphate
18g/l, magnesium sulfate 0.1g/l, calcium chloride 0.2g/l, are 46 DEG C in cultivation temperature, pH value 6.5, fermentation tank speed of agitator
120rpm, amplification culture 72h, thermophilus bacterial content >=10 into fermentation tank8Individual/ml, the thermophilic chain that then fermentation is obtained
Coccus is single, and bacterium is freeze-dried is prepared into hypopuss microbial dry powder.
（2）Bacillus subtilises：Bacillus subtilis strain on the slant medium of purification culture is seeded to and is equipped with
During volume ratio is the shaking flask of 15% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 0.2g/l, peptone 12g/
L, glucose 18g/l and Sodium Chloride 3g/l, are 36 DEG C in cultivation temperature, pH value 6.5, shaking flask rotating speed 150rpm culture 24h；Will be living
Change cultured Bacillus subtilis strain to be inoculated into equipped with the fermentation tank that volume ratio is 50% fermentation medium, volume is inoculated with
Measure as 1%, the formula of the fermentation medium is soybean cake powder 18g/l, glucose 3g/l, fish flour 3g/l, Semen Maydis powder 10g/l, phosphoric acid
Hydrogen dipotassium 0.1g/l, ammonium sulfate 0.8g/l, manganese sulfate 0.1g/l, Calcium Carbonate 5g/l and magnesium sulfate 0.1g/l, in cultivation temperature be
36 DEG C, pH value 6.5, fermentation tank speed of agitator 190rpm culture 34h, bacillus subtilis bacterial content >=10 in fermentation tank9Individual/ml,
（3）Aspergillus oryzae：Aspergillus oryzae strain on the slant medium of purification culture is seeded to equipped with the activation that volume ratio is 15%
In the shaking flask of culture medium, the formula of the activation medium is glucose 1g/l, potato juice 18g/l, magnesium sulfate 1g/l, phosphoric acid
Hydrogen dipotassium 1g/l, thiamine 0.01g/l, are 28 DEG C in cultivation temperature, pH value 5.5, shaking flask rotating speed 120rpm culture 36h；Will be living
Change cultured aspergillus oryzae strain to be inoculated into equipped with the fermentation tank that volume ratio is 50% fermentation medium, volume inoculum concentration is 1%,
The formula of the fermentation medium is sucrose 20g/l, Semen Maydis pulp 25g/l, carbamide 4g/l, magnesium sulfate 0.3g/l, potassium chloride 0.3g/
L, dipotassium hydrogen phosphate 0.8g/l and olive oil 8g/l, are 28 DEG C in cultivation temperature, pH value 5.5, fermentation tank speed of agitator 120rpm,
Culture 50h, aspergillus oryzae content >=10 in fermentation tank8Individual/ml, the freeze-dried preparation of aspergillus oryzae list bacterium that then fermentation is obtained
Into hypopuss microbial dry powder.
（4）Photosynthetic bacteria：Photosynthetic bacteria strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 15% culture medium, it is 28 DEG C in cultivation temperature, pH value 7.0, shaking flask once, cultivate 84h every stirring in 24 hours；
Activation culture good photosynthetic bacteria strain is inoculated into equipped with the fermentation tank that volume ratio is 50% culture medium, volume inoculum concentration is
6%, it is 28 DEG C in cultivation temperature, pH value 7.0 was stirred half an hour every 24 hours, speed of agitator 140rpm, is cultivated 150h, is extremely sent out
Photosynthetic bacteria content >=10 in fermentation tank8Individual/ml, then bacterium is freeze-dried is prepared into dormancy by the photosynthetic bacteria that obtains of fermentation single
Body microbial dry powder, the activation culture and fermentation when culture medium formula be dipotassium hydrogen phosphate 0.1g/l, ammonium chloride 1g/l,
Sodium Chloride 0.5g/l, sodium acetate 2g/l, sodium bicarbonate 1g/l, magnesium sulfate 0.1g/l and yeast extract 0.1g/l.
（5）Saccharomyces cerevisiae：Saccharomyces cerevisiae strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 15% culture medium, cultivation temperature be 28 DEG C, pH value 6.2, shaking flask rotating speed 140rpm, activation culture 48h；Will be living
Change cultured saccharomyces cerevisiae strain to be inoculated into equipped with the fermentation tank that volume ratio is 50% culture medium, inoculum concentration is volume ratio 1%,
The culture medium when activation culture and fermentation is beerwort potato sucrose culture medium, is 28 DEG C in cultivation temperature, pH value
6.2, fermentation tank speed of agitator 140rpm, amplification culture 84h, saccharomyces cerevisiae content >=10 into fermentation tank9Individual/ml, then will
Fermenting, the saccharomyces cerevisiae list bacterium for obtaining is freeze-dried to be prepared into hypopuss microbial dry powder.
（6）Lactobacillus casei：Species L. casei on the slant medium of purification culture is seeded to equipped with volume
In than the shaking flask for 15% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 1g/l, peptone 8g/l and chlorination
Sodium 3g/l, is 36 DEG C in cultivation temperature, and pH value 6.8, shaking flask rotating speed 180rpm cultivate 32h；By activation culture good cheese breast bar
Bacterium strain is inoculated into equipped with the fermentation tank that volume ratio is 50% fermentation medium, and volume inoculum concentration is 1%, the fermentation medium
Formula be Semen Maydis powder 13g/l, soybean cake powder 13g/l, potassium dihydrogen phosphate 0.05g/l, manganese sulfate 0.05g/l, Calcium Carbonate 15g/l and
Magnesium sulfate 0.1g/l, is 36 DEG C in cultivation temperature, pH value 6.8, and fermentation tank speed of agitator 180rpm culture 32h are done in fermentation tank
Lactobacillus paracasei content >=109Then the lactobacillus casei list bacterium drying that fermentation is obtained is prepared into hypopuss microorganism by individual/ml
Dry powder.
（7）Pseudomonas putida：Pseudomonas putida species on the slant medium of purification culture are seeded to and are equipped with
During volume ratio is the shaking flask of 15% activation medium, the formula of the activation medium be Carnis Bovis seu Bubali cream 1g/l, peptone 8g/l and
Sodium Chloride 3g/l, is 26 DEG C in cultivation temperature, and pH value 7.0, shaking flask rotating speed 120rpm cultivate 26h；By the good stench of activation culture
Pseudomonass strain is inoculated into equipped with the fermentation tank that volume ratio is 50% fermentation medium, and volume inoculum concentration is 1%, the fermentation
The formula of culture medium is glucose 3g/l, Semen Maydis powder 10g/l, fish flour 3g/l, soybean cake powder 18g/l, ammonium sulfate 0.8g/l, Calcium Carbonate
5g/l, magnesium sulfate 0.1g/l, manganese sulfate 0.1g/l and dipotassium hydrogen phosphate 0.1g/l, are 26 DEG C in cultivation temperature, and pH value 7.0 is sent out
Fermentation tank speed of agitator 180rpm cultivates 32h, pseudomonas putida content >=10 in fermentation tank9Then fermentation is obtained by individual/ml
Pseudomonas putida list bacterium drying is prepared into hypopuss microbial dry powder；
The various hypopuss microbial dry powders that High Density Cultivation is obtained, according to 10 parts of streptococcus thermophiluss, bacillus subtilises 10
The bacterium of 20 parts of part, 30 parts of aspergillus oryzae, 10 parts of photosynthetic bacteria, 20 parts of saccharomyces cerevisiae, 10 parts of lactobacillus casei and pseudomonas putida
The weight ratio planted, is mixed to get microorganism sludge deodorizer.
Embodiment 2
It is respectively that streptococcus thermophiluss, bacillus subtilises, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and stench is false
The strain of Zymomonas mobiliss carries out High Density Cultivation：
（1）Streptococcus thermophiluss：It is 30% that thermophilus strain on the slant medium of purification culture is seeded to equipped with volume ratio
Activation medium shaking flask in, the activation culture based formulas be yeast extract 8.0g/l, peptone 8.0g/l, glucose 12g/
L, dipotassium hydrogen phosphate 3g/l, Tomato juice 200ml, pH value 7.2, temperature be 50 DEG C, shaking flask rotating speed 160rpm, activation culture 30h；
Activation culture good thermophilus strain is inoculated into equipped with the fermentation tank that volume ratio is 70% fermentation medium, inoculum concentration is
Volume ratio 3%, the formula of the fermentation medium is corn cob 6g/l, glucose 12g/l, yeast extract 12g/l, potassium dihydrogen phosphate
22g/l, magnesium sulfate 0.5g/l, calcium chloride 0.6g/l, are 50 DEG C in cultivation temperature, pH value 7.2, fermentation tank speed of agitator
160rpm, amplification culture 72h, thermophilus bacterial content >=10 into fermentation tank8Individual/ml, the thermophilic chain that then fermentation is obtained
Coccus is single, and bacterium is freeze-dried is prepared into hypopuss microbial dry powder.
（2）Bacillus subtilises：Bacillus subtilis strain on the slant medium of purification culture is seeded to and is equipped with
During volume ratio is the shaking flask of 30% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 0.8g/l, peptone 18g/
L, glucose 22g/l and Sodium Chloride 7g/l, are 40 DEG C in cultivation temperature, pH value 7.5, shaking flask rotating speed 190rpm culture 16h；Will be living
Change cultured Bacillus subtilis strain to be inoculated into equipped with the fermentation tank that volume ratio is 70% fermentation medium, volume is inoculated with
Measure as 3%, the formula of the fermentation medium is soybean cake powder 22g/l, glucose 8g/l, fish flour 8g/l, Semen Maydis powder 15g/l, phosphoric acid
Hydrogen dipotassium 0.5g/l, ammonium sulfate 1.2g/l, manganese sulfate 0.3g/l, Calcium Carbonate 9g/l and magnesium sulfate 0.3g/l, in cultivation temperature be
40 DEG C, pH value 7.5, fermentation tank speed of agitator 190rpm culture 22h, bacillus subtilis bacterial content >=10 in fermentation tank9Individual/ml,
（3）Aspergillus oryzae：Aspergillus oryzae strain on the slant medium of purification culture is seeded to equipped with the activation that volume ratio is 30%
In the shaking flask of culture medium, the formula of the activation medium is glucose 5g/l, potato juice 22g/l, magnesium sulfate 2g/l, phosphoric acid
Hydrogen dipotassium 5g/l, thiamine 0.05g/l, are 30 DEG C in cultivation temperature, pH value 6.5, shaking flask rotating speed 160rpm culture 28h；Will be living
Change cultured aspergillus oryzae strain to be inoculated into equipped with the fermentation tank that volume ratio is 70% fermentation medium, volume inoculum concentration is 3%,
The formula of the fermentation medium is sucrose 40g/l, Semen Maydis pulp 35g/l, carbamide 8g/l, magnesium sulfate 0.8g/l, potassium chloride 0.8g/
L, dipotassium hydrogen phosphate 1.2g/l and olive oil 12g/l, are 30 DEG C in cultivation temperature, pH value 6.5, fermentation tank speed of agitator
160rpm, cultivates 36h, aspergillus oryzae content >=10 in fermentation tank8Individual/ml, the aspergillus oryzae list bacterium for then obtaining fermentation are chilled
Drying is prepared into hypopuss microbial dry powder.
（4）Photosynthetic bacteria：Photosynthetic bacteria strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 30% culture medium, it is 30 DEG C in cultivation temperature, pH value 7.5, shaking flask once, cultivate 64h every stirring in 24 hours；
Activation culture good photosynthetic bacteria strain is inoculated into equipped with the fermentation tank that volume ratio is 70% culture medium, volume inoculum concentration is
10%, it is 30 DEG C in cultivation temperature, pH value 7.5 was stirred half an hour every 24 hours, speed of agitator 180rpm, and culture 100 is extremely sent out
Photosynthetic bacteria content >=10 in fermentation tank8Individual/ml, then bacterium is freeze-dried is prepared into dormancy by the photosynthetic bacteria that obtains of fermentation single
Body microbial dry powder, the activation culture and fermentation when culture medium formula be dipotassium hydrogen phosphate 0.3g/l, ammonium chloride 3g/l,
Sodium Chloride 1.5g/l, sodium acetate 6g/l, sodium bicarbonate 3g/l, magnesium sulfate 0.3g/l and yeast extract 0.2g/l.
（5）Saccharomyces cerevisiae：Saccharomyces cerevisiae strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 30% culture medium, cultivation temperature be 30 DEG C, pH value 6.8, shaking flask rotating speed 180rpm, activation culture 32h；Will be living
Change cultured saccharomyces cerevisiae strain to be inoculated into equipped with the fermentation tank that volume ratio is 70% culture medium, inoculum concentration is volume ratio 3%,
The culture medium when activation culture and fermentation is beerwort potato sucrose culture medium, is 30 DEG C in cultivation temperature, pH value
6.8, fermentation tank speed of agitator 180rpm, amplification culture 68h, saccharomyces cerevisiae content >=10 into fermentation tank9Individual/ml, then will
Fermenting, the saccharomyces cerevisiae list bacterium for obtaining is freeze-dried to be prepared into hypopuss microbial dry powder.
（6）Lactobacillus casei：Species L. casei on the slant medium of purification culture is seeded to equipped with volume
In than the shaking flask for 30% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 5g/l, peptone 12g/l and chlorine
Change sodium 7g/l, be 40 DEG C in cultivation temperature, pH value 7.2, shaking flask rotating speed 220rpm cultivate 20h；By activation culture good cheese breast
Bacillus species are inoculated into equipped with the fermentation tank that volume ratio is 70% fermentation medium, and volume inoculum concentration is 3%, the fermentation culture
The formula of base is Semen Maydis powder 17g/l, soybean cake powder 17g/l, potassium dihydrogen phosphate 0.15g/l, manganese sulfate 0.1g/l, Calcium Carbonate 20g/l
With magnesium sulfate 0.2g/l, it is 40 DEG C in cultivation temperature, pH value 7.2, fermentation tank speed of agitator 220rpm cultures 20 are done in fermentation tank
Lactobacillus paracasei content >=109Then the lactobacillus casei list bacterium drying that fermentation is obtained is prepared into hypopuss microorganism by individual/ml
Dry powder.
（7）Pseudomonas putida：Pseudomonas putida species on the slant medium of purification culture are seeded to and are equipped with
During volume ratio is the shaking flask of 30% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 5g/l, peptone 12g/l
With Sodium Chloride 7g/l, it is 30 DEG C in cultivation temperature, pH value 7.5, shaking flask rotating speed 160rpm cultivate 18h；By the good evil of activation culture
Smelly pseudomonass strain is inoculated into equipped with the fermentation tank that volume ratio is 70% fermentation medium, and volume inoculum concentration is 3%, described
The formula of ferment culture medium is glucose 8g/l, Semen Maydis powder 15g/l, fish flour 8g/l, soybean cake powder 22g/l, ammonium sulfate 1.2g/l, carbonic acid
Calcium 9g/l, magnesium sulfate 0.3g/l, manganese sulfate 0.3g/l and dipotassium hydrogen phosphate 0.5g/l, are 30 DEG C in cultivation temperature, pH value 7.5,
Fermentation tank speed of agitator 200rpm cultivates 24h, pseudomonas putida content >=10 in fermentation tank9Then fermentation is obtained by individual/ml
Pseudomonas putida list bacterium drying be prepared into hypopuss microbial dry powder.
The various hypopuss microbial dry powders that High Density Cultivation is obtained, according to 20 parts of streptococcus thermophiluss, bacillus subtilis
10 parts of 15 parts of bacterium, 20 parts of aspergillus oryzae, 15 parts of photosynthetic bacteria, 10 parts of saccharomyces cerevisiae, 10 parts of lactobacillus casei and pseudomonas putida
Strain weight ratio, be mixed to get microorganism sludge deodorizer.
Embodiment 3
It is respectively that streptococcus thermophiluss, bacillus subtilises, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and stench is false
The strain of Zymomonas mobiliss carries out High Density Cultivation：
（1）Streptococcus thermophiluss：It is 22% that thermophilus strain on the slant medium of purification culture is seeded to equipped with volume ratio
Activation medium shaking flask in, the activation culture based formulas be yeast extract 7.8g/l, peptone 7.8g/l, glucose 10g/
L, dipotassium hydrogen phosphate 2g/l, Tomato juice 150ml, pH value 7.0, temperature be 48 DEG C, shaking flask rotating speed 140rpm, activation culture 36h；
Activation culture good thermophilus strain is inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, inoculum concentration is
Volume ratio 2%, the formula of the fermentation medium is corn cob 5.5g/l, glucose 10g/l, yeast extract 10g/l, biphosphate
Potassium 20g/l, magnesium sulfate 0.3g/l, calcium chloride 0.4g/l, are 49 DEG C in cultivation temperature, pH value 7.0, fermentation tank speed of agitator
140rpm, amplification culture 66h, thermophilus bacterial content >=10 into fermentation tank8Individual/ml, the thermophilic chain that then fermentation is obtained
Coccus is single, and bacterium is freeze-dried is prepared into hypopuss microbial dry powder.
（2）Bacillus subtilises：Bacillus subtilis strain on the slant medium of purification culture is seeded to and is equipped with
During volume ratio is the shaking flask of 22% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 0.5g/l, peptone 15g/
L, glucose 15g/l and Sodium Chloride 5g/l, are 38 DEG C in cultivation temperature, pH value 7.0, shaking flask rotating speed 170rpm culture 20h；Will be living
Change cultured Bacillus subtilis strain to be inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, volume is inoculated with
Measure as 2%, the formula of the fermentation medium is soybean cake powder 20g/l, glucose 5g/l, fish flour 5g/l, Semen Maydis powder 12g/l, phosphoric acid
Hydrogen dipotassium 0.3g/l, ammonium sulfate 1.0g/l, manganese sulfate 0.2g/l, Calcium Carbonate 7g/l and magnesium sulfate 0.2g/l, in cultivation temperature be
38 DEG C, pH value 7.0, fermentation tank speed of agitator 170rpm culture 28h, bacillus subtilis bacterial content >=10 in fermentation tank9Individual/ml,
（3）Aspergillus oryzae：Aspergillus oryzae strain on the slant medium of purification culture is seeded to equipped with the activation that volume ratio is 22%
In the shaking flask of culture medium, the formula of the activation medium is glucose 3g/l, potato juice 20g/l, magnesium sulfate 1.5g/l, phosphorus
Sour hydrogen dipotassium 3g/l, thiamine 0.03g/l, are 29 DEG C in cultivation temperature, pH value 6.0, shaking flask rotating speed 140rpm culture 32h；Will
The good aspergillus oryzae strain of activation culture is inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, and volume inoculum concentration is
2%, the formula of the fermentation medium is sucrose 30g/l, Semen Maydis pulp 30g/l, carbamide 6g/l, magnesium sulfate 0.5g/l, potassium chloride
0.5g/l, dipotassium hydrogen phosphate 1.0g/l and olive oil 10g/l, are 29 DEG C in cultivation temperature, pH value 6.0, fermentation tank speed of agitator
140rpm, cultivates 43h, aspergillus oryzae content >=10 in fermentation tank8Individual/ml, the aspergillus oryzae list bacterium for then obtaining fermentation are chilled
Drying is prepared into hypopuss microbial dry powder.
（4）Photosynthetic bacteria：Photosynthetic bacteria strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 22% culture medium, it is 29 DEG C in cultivation temperature, pH value 7.2, shaking flask once, cultivate 74h every stirring in 24 hours；
Activation culture good photosynthetic bacteria strain is inoculated into equipped with the fermentation tank that volume ratio is 60% culture medium, volume inoculum concentration is
8%, it is 29 DEG C in cultivation temperature, pH value 7.3 was stirred half an hour every 24 hours, speed of agitator 160rpm, is cultivated 130h, is extremely sent out
Photosynthetic bacteria content >=10 in fermentation tank8Individual/ml, then bacterium is freeze-dried is prepared into dormancy by the photosynthetic bacteria that obtains of fermentation single
Body microbial dry powder, the activation culture and fermentation when culture medium formula be dipotassium hydrogen phosphate 0.2g/l, ammonium chloride 2g/l,
Sodium Chloride 1.0g/l, sodium acetate 4g/l, sodium bicarbonate 2g/l, magnesium sulfate 0.2g/l and yeast extract 0.15g/l.
（5）Saccharomyces cerevisiae：Saccharomyces cerevisiae strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 22% culture medium, cultivation temperature be 29 DEG C, pH value 6.5, shaking flask rotating speed 160rpm, activation culture 40h；Will be living
Change cultured saccharomyces cerevisiae strain to be inoculated into equipped with the fermentation tank that volume ratio is 60% culture medium, inoculum concentration is volume ratio 2%,
The culture medium when activation culture and fermentation is beerwort potato sucrose culture medium, is 29 DEG C in cultivation temperature, pH value
6.5, fermentation tank speed of agitator 160rpm, amplification culture 76h, saccharomyces cerevisiae content >=10 into fermentation tank9Individual/ml, then will
Fermenting, the saccharomyces cerevisiae list bacterium for obtaining is freeze-dried to be prepared into hypopuss microbial dry powder.
（6）Lactobacillus casei：Species L. casei on the slant medium of purification culture is seeded to equipped with volume
In than the shaking flask for 22% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 3g/l, peptone 10g/l and chlorine
Change sodium 5g/l, be 38 DEG C in cultivation temperature, pH value 7.0, shaking flask rotating speed 200rpm cultivate 26h；By activation culture good cheese breast
Bacillus species are inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, and volume inoculum concentration is 2%, the fermentation culture
The formula of base is Semen Maydis powder 15g/l, soybean cake powder 15g/l, potassium dihydrogen phosphate 0.1g/l, manganese sulfate 0.08g/l, Calcium Carbonate 17g/l
With magnesium sulfate 0.15g/l, it is 38 DEG C in cultivation temperature, pH value 7.0, fermentation tank speed of agitator 200rpm culture 26h, in fermentation tank
Lactobacillus casei content >=109Then the lactobacillus casei list bacterium drying that fermentation is obtained is prepared into the micro- life of hypopuss by individual/ml
Thing dry powder.
（7）Pseudomonas putida：Pseudomonas putida species on the slant medium of purification culture are seeded to and are equipped with
During volume ratio is the shaking flask of 22% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 3g/l, peptone 10g/l
With Sodium Chloride 5g/l, it is 28 DEG C in cultivation temperature, pH value 7.3, shaking flask rotating speed 140rpm cultivate 22h；By the good evil of activation culture
Smelly pseudomonass strain is inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, and volume inoculum concentration is 2%, described
The formula of ferment culture medium is glucose 5g/l, Semen Maydis powder 13g/l, fish flour 5g/l, soybean cake powder 20g/l, ammonium sulfate 1.0g/l, carbonic acid
Calcium 7g/l, magnesium sulfate 0.2g/l, manganese sulfate 0.2g/l and dipotassium hydrogen phosphate 0.3g/l, are 28 DEG C in cultivation temperature, pH value 7.3,
Fermentation tank speed of agitator 190rpm cultivates 28h, pseudomonas putida content >=10 in fermentation tank9Then fermentation is obtained by individual/ml
Pseudomonas putida list bacterium drying be prepared into hypopuss microbial dry powder.
The various hypopuss microbial dry powders that High Density Cultivation is obtained, according to 15 parts of streptococcus thermophiluss, bacillus subtilis
10 parts of 12 parts of bacterium, 25 parts of aspergillus oryzae, 13 parts of photosynthetic bacteria, 15 parts of saccharomyces cerevisiae, 10 parts of lactobacillus casei and pseudomonas putida
Strain weight ratio, be mixed to get microorganism sludge deodorizer.
Embodiment 3
It is respectively that streptococcus thermophiluss, bacillus subtilises, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and stench is false
The strain of Zymomonas mobiliss carries out High Density Cultivation：
（1）Streptococcus thermophiluss：It is 22% that thermophilus strain on the slant medium of purification culture is seeded to equipped with volume ratio
Activation medium shaking flask in, the activation culture based formulas be yeast extract 7.8g/l, peptone 7.8g/l, glucose 10g/
L, dipotassium hydrogen phosphate 2g/l, Tomato juice 150ml, pH value 7.0, temperature be 48 DEG C, shaking flask rotating speed 140rpm, activation culture 36h；
Activation culture good thermophilus strain is inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, inoculum concentration is
Volume ratio 2%, the formula of the fermentation medium is corn cob 5.5g/l, glucose 10g/l, yeast extract 10g/l, biphosphate
Potassium 20g/l, magnesium sulfate 0.3g/l, calcium chloride 0.4g/l, are 49 DEG C in cultivation temperature, pH value 7.0, fermentation tank speed of agitator
140rpm, amplification culture 66h, thermophilus bacterial content >=10 into fermentation tank8Individual/ml, the thermophilic chain that then fermentation is obtained
Coccus is single, and bacterium is freeze-dried is prepared into hypopuss microbial dry powder.
（2）Bacillus subtilises：Bacillus subtilis strain on the slant medium of purification culture is seeded to and is equipped with
During volume ratio is the shaking flask of 22% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 0.5g/l, peptone 15g/
L, glucose 15g/l and Sodium Chloride 5g/l, are 38 DEG C in cultivation temperature, pH value 7.0, shaking flask rotating speed 170rpm culture 20h；Will be living
Change cultured Bacillus subtilis strain to be inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, volume is inoculated with
Measure as 2%, the formula of the fermentation medium is soybean cake powder 20g/l, glucose 5g/l, fish flour 5g/l, Semen Maydis powder 12g/l, phosphoric acid
Hydrogen dipotassium 0.3g/l, ammonium sulfate 1.0g/l, manganese sulfate 0.2g/l, Calcium Carbonate 7g/l and magnesium sulfate 0.2g/l, in cultivation temperature be
38 DEG C, pH value 7.0, fermentation tank speed of agitator 170rpm culture 28h, bacillus subtilis bacterial content >=10 in fermentation tank9Individual/ml,
（3）Aspergillus oryzae：Aspergillus oryzae strain on the slant medium of purification culture is seeded to equipped with the activation that volume ratio is 22%
In the shaking flask of culture medium, the formula of the activation medium is glucose 3g/l, potato juice 20g/l, magnesium sulfate 1.5g/l, phosphorus
Sour hydrogen dipotassium 3g/l, thiamine 0.03g/l, are 29 DEG C in cultivation temperature, pH value 6.0, shaking flask rotating speed 140rpm culture 32h；Will
The good aspergillus oryzae strain of activation culture is inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, and volume inoculum concentration is
2%, the formula of the fermentation medium is sucrose 30g/l, Semen Maydis pulp 30g/l, carbamide 6g/l, magnesium sulfate 0.5g/l, potassium chloride
0.5g/l, dipotassium hydrogen phosphate 1.0g/l and olive oil 10g/l, are 29 DEG C in cultivation temperature, pH value 6.0, fermentation tank speed of agitator
140rpm, cultivates 43h, aspergillus oryzae content >=10 in fermentation tank8Individual/ml, the aspergillus oryzae list bacterium for then obtaining fermentation are chilled
Drying is prepared into hypopuss microbial dry powder.
（4）Photosynthetic bacteria：Photosynthetic bacteria strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 22% culture medium, it is 29 DEG C in cultivation temperature, pH value 7.2, shaking flask once, cultivate 74h every stirring in 24 hours；
Activation culture good photosynthetic bacteria strain is inoculated into equipped with the fermentation tank that volume ratio is 60% culture medium, volume inoculum concentration is
8%, it is 29 DEG C in cultivation temperature, pH value 7.3 was stirred half an hour every 24 hours, speed of agitator 160rpm, is cultivated 130h, is extremely sent out
Photosynthetic bacteria content >=10 in fermentation tank8Individual/ml, then bacterium is freeze-dried is prepared into dormancy by the photosynthetic bacteria that obtains of fermentation single
Body microbial dry powder, the activation culture and fermentation when culture medium formula be dipotassium hydrogen phosphate 0.2g/l, ammonium chloride 2g/l,
Sodium Chloride 1.0g/l, sodium acetate 4g/l, sodium bicarbonate 2g/l, magnesium sulfate 0.2g/l and yeast extract 0.15g/l.
（5）Saccharomyces cerevisiae：Saccharomyces cerevisiae strain on the slant medium of purification culture is seeded to and equipped with volume ratio is
In the shaking flask of 22% culture medium, cultivation temperature be 29 DEG C, pH value 6.5, shaking flask rotating speed 160rpm, activation culture 40h；Will be living
Change cultured saccharomyces cerevisiae strain to be inoculated into equipped with the fermentation tank that volume ratio is 60% culture medium, inoculum concentration is volume ratio 2%,
The culture medium when activation culture and fermentation is beerwort potato sucrose culture medium, is 29 DEG C in cultivation temperature, pH value
6.5, fermentation tank speed of agitator 160rpm, amplification culture 76h, saccharomyces cerevisiae content >=10 into fermentation tank9Individual/ml, then will
Fermenting, the saccharomyces cerevisiae list bacterium for obtaining is freeze-dried to be prepared into hypopuss microbial dry powder.
（6）Lactobacillus casei：Species L. casei on the slant medium of purification culture is seeded to equipped with volume
In than the shaking flask for 22% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 3g/l, peptone 10g/l and chlorine
Change sodium 5g/l, be 38 DEG C in cultivation temperature, pH value 7.0, shaking flask rotating speed 200rpm cultivate 26h；By activation culture good cheese breast
Bacillus species are inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, and volume inoculum concentration is 2%, the fermentation culture
The formula of base is Semen Maydis powder 15g/l, soybean cake powder 15g/l, potassium dihydrogen phosphate 0.1g/l, manganese sulfate 0.08g/l, Calcium Carbonate 17g/l
With magnesium sulfate 0.15g/l, it is 38 DEG C in cultivation temperature, pH value 7.0, fermentation tank speed of agitator 200rpm culture 26h, in fermentation tank
Lactobacillus casei content >=109Then the lactobacillus casei list bacterium drying that fermentation is obtained is prepared into the micro- life of hypopuss by individual/ml
Thing dry powder.
（7）Pseudomonas putida：Pseudomonas putida species on the slant medium of purification culture are seeded to and are equipped with
During volume ratio is the shaking flask of 22% activation medium, the formula of the activation medium is Carnis Bovis seu Bubali cream 3g/l, peptone 10g/l
With Sodium Chloride 5g/l, it is 28 DEG C in cultivation temperature, pH value 7.3, shaking flask rotating speed 140rpm cultivate 22h；By the good evil of activation culture
Smelly pseudomonass strain is inoculated into equipped with the fermentation tank that volume ratio is 60% fermentation medium, and volume inoculum concentration is 2%, described
The formula of ferment culture medium is glucose 5g/l, Semen Maydis powder 13g/l, fish flour 5g/l, soybean cake powder 20g/l, ammonium sulfate 1.0g/l, carbonic acid
Calcium 7g/l, magnesium sulfate 0.2g/l, manganese sulfate 0.2g/l and dipotassium hydrogen phosphate 0.3g/l, are 28 DEG C in cultivation temperature, pH value 7.3,
Fermentation tank speed of agitator 190rpm cultivates 28h, pseudomonas putida content >=10 in fermentation tank9Then fermentation is obtained by individual/ml
Pseudomonas putida list bacterium drying be prepared into hypopuss microbial dry powder.
The various hypopuss microbial dry powders that High Density Cultivation is obtained, according to 15 parts of streptococcus thermophiluss, bacillus subtilis
10 parts of 12 parts of bacterium, 25 parts of aspergillus oryzae, 13 parts of photosynthetic bacteria, 15 parts of saccharomyces cerevisiae, 10 parts of lactobacillus casei and pseudomonas putida
Strain weight ratio, be mixed to get microorganism sludge deodorizer.
It should be noted that above-described embodiment, not for limiting protection scope of the present invention, in above-mentioned technical proposal
On the basis of done equivalents or replacement each fall within the scope protected by the claims in the present invention.
Claims (10)
1. a kind of microorganism sludge deodorizer, it is characterised in that the deodorizer is made up of the strain of following mass parts, thermophilic chain
10～20 parts of coccus, 10～15 parts of bacillus subtilises, 20～30 parts of aspergillus oryzae, 10～15 parts of photosynthetic bacteria, saccharomyces cerevisiae 10
～20 parts, 10～15 parts of lactobacillus casei, and 10～20 parts of pseudomonas putida.
2. microorganism sludge deodorizer according to claim 1, it is characterised in that the streptococcus thermophiluss deposit number is
CGMCC1.3996, bacillus subtilises deposit number be CGMCC1.1160, aspergillus oryzae deposit number be CGMCC3.4437, light
It is CGMCC1.7520 to close antibacterial deposit number, and saccharomyces cerevisiae deposit number is CGMCC2.1793, lactobacillus casei deposit number
It is that CGMCC1.3113 and pseudomonas putida deposit number are CGMCC1.2309.
3. the preparation method of microorganism sludge deodorizer described in claim 1 or 2, it is characterised in that methods described is as follows,
1）Will be streptococcus thermophiluss, bacillus subtilises, aspergillus oryzae, photosynthetic bacteria, saccharomyces cerevisiae, lactobacillus casei and stench false single
The strain of born of the same parents bacterium carries out High Density Cultivation；
2）Above-mentioned strain is seeded to into activation culture in the shaking flask equipped with the culture medium that volume ratio is 15～30% respectively, will culture
Good strain is inoculated in fermentation tank and carries out fermentation amplification culture, will be enlarged by culture and obtains various single bacterium dehydrate, is prepared into
Hypopuss microbial dry powder；
3）By step 2）In mycopowder according to 10～20 parts of streptococcus thermophiluss, 10～15 parts of bacillus subtilises, aspergillus oryzae 20～
30 parts, 10～15 parts of photosynthetic bacteria, 10～20 parts of saccharomyces cerevisiae, 10～15 parts of lactobacillus casei and pseudomonas putida 10～20
The weight ratio of part, is mixed to get microorganism sludge deodorizer.
4. the preparation method of microorganism sludge deodorizer according to claim 3, it is characterised in that step 2）Middle thermophilus
The preparation of bacterium hypopuss microbial dry powder is comprised the following steps：
Thermophilus strain on the slant medium of purification culture is seeded to equipped with the activation culture that volume ratio is 15～30%
In the shaking flask of base, the activation culture based formulas be 6.5～8.0g/l of yeast extract, 6.5～8.0g/l of peptone, glucose 8～
12g/l, 1～3g/l of dipotassium hydrogen phosphate, 50～200ml of Tomato juice, pH value 6.3～7.2, temperature are 46～50 DEG C, shaking flask turn
Speed 120～160rpm, 30～42h of activation culture；
Activation culture good thermophilus strain is inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium,
Inoculum concentration is volume ratio 1～3%, and the formula of the fermentation medium is 5～6g/l of corn cob, 8～12g/l of glucose, yeast extract
8～12g/l, 18～22g/l of potassium dihydrogen phosphate, 0.1～0.5g/l of magnesium sulfate, 0.2～0.6g/l of calcium chloride, in cultivation temperature be
48～50 DEG C, pH value 6.3～7.2, fermentation tank 120～160rpm of speed of agitator, 60～72h of amplification culture are thermophilic into fermentation tank
Hot hammer bacterial content >=108Individual/ml, then to be prepared into hypopuss micro- by the streptococcus thermophiluss list bacterium that obtains of fermentation freeze-dried
Biological dry powder.
5. according to claim 3 or 4 microorganism sludge deodorizer preparation method, it is characterised in that step 2）Middle hay
The preparation of the hypopuss microbial dry powder of bacillus cereuss is comprised the following steps：
Bacillus subtilis strain on the slant medium of purification culture is seeded to equipped with the activation that volume ratio is 15～30%
In the shaking flask of culture medium, the formula of the activation medium is 0.2～0.8g/l of Carnis Bovis seu Bubali cream, 12～18g/l of peptone, glucose
3～7g/l of 18～22g/l and Sodium Chloride, is 36～40 DEG C in cultivation temperature, pH value 6.5～7.5, shaking flask rotating speed 150～
190rpm cultivates 16～24h；
The good Bacillus subtilis strain of activation culture is inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium
Interior, volume inoculum concentration is 1～3%, and the formula of the fermentation medium is 18～22g/l of soybean cake powder, 3～8g/l of glucose, fish flour
3～8g/l, 10～15g/l of Semen Maydis powder, 0.1～0.5g/l of dipotassium hydrogen phosphate, 0.8～1.2g/l of ammonium sulfate, manganese sulfate 0.1～
0.3g/l, 0.1～0.3g/l of 5～9g/l of Calcium Carbonate and magnesium sulfate, are 36～40 DEG C in cultivation temperature, and pH value 6.5～7.5 is sent out
150～190rpm of fermentation tank speed of agitator cultivates 22～34h, bacillus subtilis bacterial content >=10 in fermentation tank9Individual/ml, then will
The bacillus subtilises that obtain of fermentation are single, and bacterium is freeze-dried is prepared into hypopuss microbial dry powder.
6. the preparation method of microorganism sludge deodorizer according to claim 5, it is characterised in that step 2）Middle aspergillus oryzae
The preparation of hypopuss microbial dry powder is comprised the following steps：
Aspergillus oryzae strain on the slant medium of purification culture is seeded to equipped with the activation medium that volume ratio is 15～30%
Shaking flask in, the formula of the activation medium be 1～5g/l of glucose, 18～22g/l of potato juice, 1～2g/l of magnesium sulfate,
1～5g/l of dipotassium hydrogen phosphate, 0.01～0.05g/l of thiamine, are 28～30 DEG C in cultivation temperature, pH value 5.5～6.5, shaking flask
120～160rpm of rotating speed cultivates 28～36h；
Activation culture good aspergillus oryzae strain is inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium, body
Product inoculum concentration is 1～3%, the formula of the fermentation medium be 20～40g/l of sucrose, 25～35g/l of Semen Maydis pulp, carbamide 4～
8g/l, 0.3～0.8g/l of magnesium sulfate, 0.3～0.8g/l of potassium chloride, 8～12g/ of 0.8～1.2g/l of dipotassium hydrogen phosphate and olive oil
L, is 28～30 DEG C in cultivation temperature, and pH value 5.5～6.5, fermentation tank 120～160rpm of speed of agitator cultivate 36～50h, send out
Aspergillus oryzae content >=10 in fermentation tank8Individual/ml, then to be prepared into hypopuss micro- by the aspergillus oryzae list bacterium that obtains of fermentation freeze-dried
Biological dry powder.
7. the preparation method of microorganism sludge deodorizer according to claim 6, it is characterised in that step 2）Middle photosynthetic bacteria
The preparation of hypopuss microbial dry powder comprise the following steps：
Photosynthetic bacteria strain on the slant medium of purification culture is seeded to equipped with the culture medium that volume ratio is 15～30%
In shaking flask, it is 28～30 DEG C in cultivation temperature, pH value 7.0～7.5, shaking flask once, cultivate 64～84h every stirring in 24 hours；
Activation culture good photosynthetic bacteria strain is inoculated into equipped with the fermentation tank that volume ratio is 50～70% culture medium, volume is inoculated with
Measure as 6～10%, be 28～30 DEG C in cultivation temperature, pH value 7.0～7.5 stirred half an hour, speed of agitator 140 every 24 hours
～180rpm, cultivates 100～150h, photosynthetic bacteria content >=10 into fermentation tank8Individual/ml, what is then obtained fermenting are photosynthetic
Antibacterial list bacterium is freeze-dried to be prepared into hypopuss microbial dry powder, and the activation culture with the formula of culture medium when fermenting is
0.1～0.3g/l of dipotassium hydrogen phosphate, 1～3g/l of ammonium chloride, 0.5～1.5g/l of Sodium Chloride, 2～6g/l of sodium acetate, sodium bicarbonate 1
～3g/l, 0.1～0.2g/l of 0.1～0.3g/l of magnesium sulfate and yeast extract.
8. the preparation method of microorganism sludge deodorizer according to claim 7, it is characterised in that step 2）Middle saccharomyces cerevisiae
The preparation of hypopuss microbial dry powder is comprised the following steps：
Saccharomyces cerevisiae strain on the slant medium of purification culture is seeded to equipped with the culture medium that volume ratio is 15～30%
In shaking flask, cultivation temperature be 28～30 DEG C, pH value 6.2～6.8, shaking flask 140～180rpm of rotating speed, 32～48h of activation culture；
Activation culture good saccharomyces cerevisiae strain is inoculated into equipped with the fermentation tank that volume ratio is 50～70% culture medium, inoculum concentration is
Culture medium when volume ratio 1～3%, the activation culture and fermentation is beerwort potato sucrose culture medium, in cultivation temperature is
28～30 DEG C, pH value 6.2～6.8, fermentation tank 140～180rpm of speed of agitator, 68～84h of amplification culture are made into fermentation tank
Brewer yeast content >=109Individual/ml, the saccharomyces cerevisiae list bacterium for then obtaining fermentation is freeze-dried to be prepared into hypopuss microorganism
Dry powder.
9. the preparation method of microorganism sludge deodorizer according to claim 8, it is characterised in that step 2）Middle cheese breast bar
The preparation of the hypopuss microbial dry powder of bacterium is comprised the following steps：
Species L. casei on the slant medium of purification culture is seeded to equipped with the activation training that volume ratio is 15～30%
In the shaking flask of foster base, the formula of the activation medium is 1～5g/l of Carnis Bovis seu Bubali cream, 3～7g/ of 8～12g/l of peptone and Sodium Chloride
L, is 36～40 DEG C in cultivation temperature, and pH value 6.8～7.2, shaking flask 180～220rpm of rotating speed cultivate 20～32h；
The good species L. casei of activation culture is inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium
Interior, volume inoculum concentration is 1～3%, and the formula of the fermentation medium is 13～17g/l of Semen Maydis powder, 13～17g/l of soybean cake powder, phosphorus
Acid dihydride 0.05～0.15g/l of potassium, 0.05～0.1g/l of manganese sulfate, 0.1～0.2g/l of 15～20g/l of Calcium Carbonate and magnesium sulfate,
It it is 36～40 DEG C in cultivation temperature, pH value 6.8～7.2,180～220rpm of fermentation tank speed of agitator cultivate 20～32h, fermentation tank
Middle lactobacillus casei content >=109Then the lactobacillus casei list bacterium drying that fermentation is obtained is prepared into hypopuss micro- by individual/ml
Biological dry powder；
Step 2）The preparation of the hypopuss microbial dry powder of middle pseudomonas putida is comprised the following steps：
Pseudomonas putida species on the slant medium of purification culture are seeded to equipped with the activation that volume ratio is 15～30%
In the shaking flask of culture medium, the formula of the activation medium be 1～5g/l of Carnis Bovis seu Bubali cream, 8～12g/l of peptone and Sodium Chloride 3～
7g/l, is 26～30 DEG C in cultivation temperature, and pH value 7.0～7.5, shaking flask 120～160rpm of rotating speed cultivate 18～26h；
The good Pseudomonas putida species of activation culture are inoculated into equipped with the fermentation tank that volume ratio is 50～70% fermentation medium
Interior, volume inoculum concentration is 1～3%, and the formula of the fermentation medium is 3～8g/l of glucose, 10～15g/l of Semen Maydis powder, fish flour
3～8g/l, 18～22g/l of soybean cake powder, 0.8～1.2g/l of ammonium sulfate, 5～9g/l of Calcium Carbonate, 0.1～0.3g/l of magnesium sulfate, sulfur
Sour 0.1～0.3g/l of manganese and 0.1～0.5g/l of dipotassium hydrogen phosphate, is 26～30 DEG C in cultivation temperature, pH value 7.0～7.5, fermentation
180～200rpm of tank speed of agitator cultivates 24～32h, pseudomonas putida content >=10 in fermentation tank9Individual/ml, then will send out
The pseudomonas putida list bacterium drying that ferment is obtained is prepared into hypopuss microbial dry powder.
10. the microorganism sludge deodorizer described in claim 1-9 any one is used for municipal sludge deodorization.
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