CN111961628A - Preparation method and application method of microbial deodorant - Google Patents
Preparation method and application method of microbial deodorant Download PDFInfo
- Publication number
- CN111961628A CN111961628A CN202010881585.8A CN202010881585A CN111961628A CN 111961628 A CN111961628 A CN 111961628A CN 202010881585 A CN202010881585 A CN 202010881585A CN 111961628 A CN111961628 A CN 111961628A
- Authority
- CN
- China
- Prior art keywords
- microbial deodorant
- preparing
- fermentation
- cfu
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention discloses a preparation method of a microbial deodorant, which comprises the steps of preparing seed liquid; preparing a fermentation medium; preparing a microbial deodorant. The invention also discloses a use method of the microbial deodorant. Compared with the prior art, the microbial deodorant prepared by the preparation method of the microbial deodorant has the advantages of high deodorization efficiency, low cost, no secondary pollution and good disinfection effect. The application method of the microbial deodorant is simple and convenient to operate, low in cost and high in deodorization efficiency.
Description
Technical Field
The invention relates to the technical field of microbial deodorization, in particular to a preparation method and a use method of a microbial deodorant.
Background
With the development of society, the yield of urban household garbage is increasing day by day, the proportion of wet garbage in the household garbage is gradually increased, and the defects of the traditional landfill type household garbage treatment mode are obvious day by day, so that the garbage classification becomes a necessary policy. In China, dry and wet garbage classification is carried out nationwide from 2019, but organic substances such as cellulose, protein and starch in the classified wet garbage are excessively enriched, the wet garbage is more prone to decay than before after water absorption and dilution of the dry garbage are lacked, particularly, wet garbage sites for collecting wet garbage in a vegetable market are foul, and bad influence is caused on life and bodies of workers and surrounding residents. And various pathogenic bacteria are easier to breed. Each wet garbage site is complained of surrounding residents, and is faced with closing, and the correct policy of dry and wet garbage classification is seriously tested.
At present, the research on deodorization mainly focuses on three aspects of physical deodorization, chemical deodorization and biological deodorization, and the biological deodorization is the main research direction at present because the principle of the biological deodorization is to radically eliminate the generation of odor, so that the research is directed to high deodorization efficiency, simple operation, low cost and no secondary pollution.
Disclosure of Invention
The invention aims to overcome the technical problems and provides a preparation method and a using method of a microbial deodorant.
The invention provides a preparation method of a microbial deodorant, which comprises the following steps:
preparing seed liquid;
preparing a fermentation medium;
preparing a microbial deodorant.
Preferably, the preparation of the seed liquid comprises:
respectively preparing culture medium of 3 seed solutions according to the inoculation amounts of lactobacillus plantarum 10% v/v, saccharomyces cerevisiae 5% v/v and bacillus subtilis 10% v/v, inoculating, and culturing at 35 ℃ for 24h to obtain fermented seed solution.
Preferably, the preparation of the fermentation medium: 3 wt% of tomato juice, 2 wt% of wheat bran juice, 10 wt% of molasses, 0.1 wt% of peptone, 0.1 wt% of yeast extract and the balance of water, wherein the pH value is 6.0-6.5, the total volume in a tank is not more than 70%, and the tomato juice and the wheat bran juice are sterilized in a fermentation tank at 121 ℃ for 30 min.
Preferably, the preparation of the microbial deodorant comprises:
when the temperature of the fermentation culture medium is cooled to 30-38 ℃, saccharomyces cerevisiae accounting for 5% v/v of the total volume of the fermentation culture medium and bacillus subtilis accounting for 10% v/v of the total volume of the fermentation culture medium are inoculated, and the culture conditions are as follows: the temperature is 30-38 ℃, the stirring speed is 180-220 r/min, the ventilation volume is 0.3-0.5: 1v/v, the tank pressure is 0.03-0.04 MPa, the fermentation is 24-32 h, when the enzyme activity of the cellulase is 80-100U/ml, the enzyme activity of the acid pectinase is 3000-4000U/ml, and the enzyme activity of the saccharomyces cerevisiae is 2 multiplied by 107～5×107CFU/ml, Bacillus subtilis 4X 108～6×108When the concentration of the lactobacillus plantarum is CFU/ml, inoculating lactobacillus plantarum seed liquid accounting for 10% v/v of the total volume of the culture medium, and standing and culturing for 24-36 h in an anoxic manner at the temperature of 35-37 ℃ when the saccharomyces cerevisiae is 5 multiplied by 107～1.0×108CFU/ml, Lactobacillus plantarum 2X 108～5×108CFU/ml, Bacillus subtilis 2X 109～5×109CFU/ml, pH3.5-4.0, to obtain the microbial deodorant.
Preferably, the preparation method further comprises: after the microbial deodorant is prepared, acid lipase, acid protease and low-temperature amylase are added according to enzyme activities, wherein the enzyme activities are respectively 1000-2000U/ml of acid lipase, 1000-2000U/ml of acid protease and 28-35U/ml of low-temperature amylase.
The invention also provides a using method of the microbial deodorant, and the microbial deodorant prepared by the preparation method of the microbial deodorant is diluted by water by 20-30 times and then used.
Preferably, the microbial deodorant is diluted with water and sprayed in an amount of 5L per cubic meter.
Compared with the prior art, the invention provides the method for treating the liver cancer.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a preparation method of a microbial deodorant, which comprises the following steps:
preparing seed liquid;
preparing a fermentation medium;
preparing a microbial deodorant.
In this embodiment, the preparation of the seed solution includes:
respectively preparing culture medium of 3 seed solutions according to the inoculation amounts of lactobacillus plantarum 10% v/v, saccharomyces cerevisiae 5% v/v and bacillus subtilis 10% v/v, inoculating, and culturing at 35 ℃ for 24h to obtain fermented seed solution.
Specifically, the preparation of the fermentation medium comprises the following steps: 3 wt% of tomato juice, 2 wt% of wheat bran juice, 10 wt% of molasses, 0.1 wt% of peptone, 0.1 wt% of yeast extract and the balance of water, wherein the pH value is 6.0-6.5, the total volume in a tank is not more than 70%, and the tomato juice and the wheat bran juice are sterilized in a fermentation tank at 121 ℃ for 30 min.
Specifically, the preparation of the microbial deodorant comprises the following steps:
when the temperature of the fermentation culture medium is cooled to 30-38 ℃, saccharomyces cerevisiae accounting for 5% v/v of the total volume of the fermentation culture medium and bacillus subtilis accounting for 10% v/v of the total volume of the fermentation culture medium are inoculated, and the culture conditions are as follows: the temperature is 30-38 ℃, the stirring speed is 180-220 r/min, the ventilation volume is 0.3-0.5: 1v/v, the tank pressure is 0.03-0.04 MPa, the fermentation is 24-32 h, when the enzyme activity of the cellulase is 80-100U/ml, the enzyme activity of the acid pectinase is 3000-4000U/ml, and the enzyme activity of the saccharomyces cerevisiae is 2 multiplied by 107～5×107CFU/ml, Bacillus subtilis 4X 108～6×108When the concentration of the lactobacillus plantarum is CFU/ml, inoculating lactobacillus plantarum seed liquid accounting for 10% v/v of the total volume of the culture medium, and standing and culturing for 24-36 h in an anoxic manner at the temperature of 35-37 ℃ when the saccharomyces cerevisiae is 5 multiplied by 107～1.0×108CFU/ml, Lactobacillus plantarum 2X 108～5×108CFU/ml, Bacillus subtilis 2X 109～5×109CFU/ml, pH3.5-4.0, to obtain the microbial deodorant.
More specifically, the preparation method further comprises the following steps: after the microbial deodorant is prepared, acid lipase, acid protease and low-temperature amylase are added according to enzyme activities, wherein the enzyme activities are respectively 1000-2000U/ml of acid lipase, 1000-2000U/ml of acid protease and 28-35U/ml of low-temperature amylase.
The invention also provides a using method of the microbial deodorant, and the microbial deodorant prepared by the preparation method of the microbial deodorant is diluted by water by 20-30 times and then used.
In the embodiment, the microbial deodorant is diluted with water and then sprayed in an amount of 5L per cubic meter.
Example one
Preparing a seed solution: respectively preparing culture medium of 3 seed solutions according to the inoculation amounts of lactobacillus plantarum 10% v/v, saccharomyces cerevisiae 5% v/v and bacillus subtilis 10% v/v, inoculating, and culturing at 35 ℃ for 24h to obtain fermented seed solution.
Preparation of a fermentation medium: 3 percent of tomato juice, 2 percent of wheat bran juice, 10 percent of molasses, 0.1 percent of peptone, 0.1 percent of yeast extract and the balance of water, the pH value is 6.0, the total volume of the canned food is 65 percent, and the canned food is sterilized in a fermentation tank for 30min at the temperature of 121 ℃.
Preparing a microbial deodorant:
when the temperature of the fermentation culture medium is cooled to 30 ℃, saccharomyces cerevisiae accounting for 5% v/v of the total volume of the fermentation culture medium and bacillus subtilis accounting for 10% v/v of the total volume of the fermentation culture medium are inoculated, and the culture conditions are as follows: the temperature is 30 ℃, the stirring speed is 180r/min, the ventilation volume is 0.3-0.5: 1v/v, the tank pressure is 0.03MPa, the fermentation is carried out for 24 hours, when the enzyme activity of the cellulase is 80-100U/ml, the enzyme activity of the acid pectinase is 3000-4000U/ml, and the saccharomyces cerevisiae is 2 multiplied by 107～5×107CFU/ml, Bacillus subtilis 4X 108～6×108Inoculating Lactobacillus plantarum seed solution 10% v/v of the total volume of the culture medium at CFU/ml, and culturing at 35 deg.C under anaerobic condition for 24 hr when Saccharomyces cerevisiae is 5 × 107～1.0×108CFU/ml, Lactobacillus plantarum 2X 108～5×108CFU/ml, Bacillus subtilis 2X 109～5×109CFU/ml, pH3.5,obtaining the microbial deodorant.
After the microbial deodorant is prepared, acid lipase, acid protease and low-temperature amylase are added according to enzyme activities, wherein the enzyme activities are respectively 1000-2000U/ml of acid lipase, 1000-2000U/ml of acid protease and 28-35U/ml of low-temperature amylase.
The microbial deodorant is diluted 20 times with water for use.
The microbial deodorant is diluted with water and sprayed in an amount of 5L per cubic meter.
Example two
Preparing a seed solution: respectively preparing culture medium of 3 seed solutions according to the inoculation amounts of lactobacillus plantarum 10% v/v, saccharomyces cerevisiae 5% v/v and bacillus subtilis 10% v/v, inoculating, and culturing at 35 ℃ for 24h to obtain fermented seed solution.
Preparation of a fermentation medium: 3 percent of tomato juice, 2 percent of wheat bran juice, 10 percent of molasses, 0.1 percent of peptone, 0.1 percent of yeast extract and the balance of water, the pH value is 6.5, the total volume of the canned food is 68 percent, and the canned food is sterilized in a fermentation tank for 30min at the temperature of 121 ℃.
Preparing a microbial deodorant:
when the temperature of the fermentation culture medium is cooled to 38 ℃, saccharomyces cerevisiae accounting for 5% v/v of the total volume of the fermentation culture medium and bacillus subtilis accounting for 10% v/v of the total volume of the fermentation culture medium are inoculated, and the culture conditions are as follows: the temperature is 38 ℃, the stirring speed is 220r/min, the ventilation volume is 0.3-0.5: 1v/v, the tank pressure is 0.04MPa, the fermentation is carried out for 32 hours, when the enzyme activity of the cellulase is 80-100U/ml, the enzyme activity of the acid pectinase is 3000-4000U/ml, and the saccharomyces cerevisiae is 2 multiplied by 107～5×107CFU/ml, Bacillus subtilis 4X 108～6×108Inoculating Lactobacillus plantarum seed solution 10% v/v of the total volume of the culture medium at CFU/ml, and culturing at 37 deg.C under anaerobic condition for 36 hr when Saccharomyces cerevisiae is 5 × 107～1.0×108CFU/ml, Lactobacillus plantarum 2X 108～5×108CFU/ml, Bacillus subtilis 2X 109～5×109CFU/ml, pH4.0, to obtain the microbial deodorant.
The microbial deodorant is diluted 30 times with water for use.
EXAMPLE III
Preparing a seed solution: respectively preparing culture medium of 3 seed solutions according to the inoculation amounts of lactobacillus plantarum 10% v/v, saccharomyces cerevisiae 5% v/v and bacillus subtilis 10% v/v, inoculating, and culturing at 35 ℃ for 24h to obtain fermented seed solution.
Preparation of a fermentation medium: 3 percent of tomato juice, 2 percent of wheat bran juice, 10 percent of molasses, 0.1 percent of peptone, 0.1 percent of yeast extract and the balance of water, the pH value is 6.2, the total volume of the canned food is 70 percent, and the canned food is sterilized in a fermentation tank for 30min at the temperature of 121 ℃.
Preparing a microbial deodorant:
when the temperature of the fermentation culture medium is cooled to 35 ℃, saccharomyces cerevisiae accounting for 5% v/v of the total volume of the fermentation culture medium and bacillus subtilis accounting for 10% v/v of the total volume of the fermentation culture medium are inoculated, and the culture conditions are as follows: the temperature is 35 ℃, the stirring speed is 200r/min, the ventilation volume is 0.3-0.5: 1v/v, the tank pressure is 0.03MPa, the fermentation is carried out for 30 hours, when the enzyme activity of the cellulase is 80-100U/ml, the enzyme activity of the acid pectinase is 3000-4000U/ml, and the saccharomyces cerevisiae is 2 multiplied by 107～5×107CFU/ml, Bacillus subtilis 4X 108～6×108Inoculating Lactobacillus plantarum seed solution 10% v/v of the total volume of the culture medium at CFU/ml, and culturing at 36 deg.C under anaerobic condition for 30 hr when Saccharomyces cerevisiae is 5 × 107～1.0×108CFU/ml, Lactobacillus plantarum 2X 108～5×108CFU/ml, Bacillus subtilis 2X 109～5×109CFU/ml, pH3.8, get the microbial deodorant.
After the microbial deodorant is prepared, acid lipase, acid protease and low-temperature amylase are added according to enzyme activities, wherein the enzyme activities are respectively 1000-2000U/ml of acid lipase, 1000-2000U/ml of acid protease and 28-35U/ml of low-temperature amylase.
The microbial deodorant is diluted 25 times with water for use.
The microbial deodorant is diluted with water and sprayed in an amount of 5L per cubic meter.
The third-party detection mechanism for the microbial deodorant delivery inspection is used for detecting according to the Row label CJ/T516-2017/6.9, the deodorization efficiency of hydrogen sulfide reaches 97.2%, the ammonia removal rate reaches 93.6%, and the sulfur dioxide reaches 92.6%.
Compared with the prior art, the microbial deodorant prepared by the preparation method of the microbial deodorant provided by the invention has high deodorization efficiency, low cost and no secondary pollution, and particularly, acidic cellulase, acidic pectinase, acidic lipase, acidic protease and low-temperature amylase in the deodorant solution are utilized to accelerate the decomposition of organic matters in wet garbage to provide nutrients for the growth of microorganisms, and saccharomyces cerevisiae production metabolism provides early-stage easily-absorbed sugar for lactobacillus plantarum and bacillus, so that the bacillus and lactobacillus plantarum can be rapidly propagated in garbage and release special aromatic odor, and when the pH of the garbage is 5-6.5, the bacillus is rapidly propagated and metabolizes a large amount of acidic cellulase and acidic pectinase to promote the rapid softening and decomposition of solid matters such as fibers in the wet garbage, thereby indirectly increasing the loading capacity of a garbage can. Meanwhile, the lactobacillus plantarum has a good inhibition effect on some putrefying bacteria and low-temperature bacteria. The lactobacillus plantarum is propagated in a large quantity, and a large quantity of organic acid is metabolized to reduce the pH value of the wet garbage, so that the production of bacteria sensitive to acidic conditions, such as escherichia coli, salmonella and the like, can be effectively inhibited.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (7)
1. A preparation method of a microbial deodorant is characterized by comprising the following steps:
preparing seed liquid;
preparing a fermentation medium;
preparing a microbial deodorant.
2. The method for preparing a microbial deodorant according to claim 1, wherein the preparation of the seed liquid comprises:
respectively preparing culture medium of 3 seed solutions according to the inoculation amounts of lactobacillus plantarum 10% v/v, saccharomyces cerevisiae 5% v/v and bacillus subtilis 10% v/v, inoculating, and culturing at 35 ℃ for 24h to obtain fermented seed solution.
3. The method for producing a microbial deodorant according to claim 2, wherein the production of the fermentation medium: 3 wt% of tomato juice, 2 wt% of wheat bran juice, 10 wt% of molasses, 0.1 wt% of peptone, 0.1 wt% of yeast extract and the balance of water, wherein the pH value is 6.0-6.5, the total volume in a tank is not more than 70%, and the tomato juice and the wheat bran juice are sterilized in a fermentation tank at 121 ℃ for 30 min.
4. The method of preparing a microbial deodorant according to claim 3, wherein the preparation of the microbial deodorant comprises:
when the temperature of the fermentation culture medium is cooled to 30-38 ℃, saccharomyces cerevisiae accounting for 5% v/v of the total volume of the fermentation culture medium and bacillus subtilis accounting for 10% v/v of the total volume of the fermentation culture medium are inoculated, and the culture conditions are as follows: the temperature is 30-38 ℃, the stirring speed is 180-220 r/min, the ventilation volume is 0.3-0.5: 1v/v, the tank pressure is 0.03-0.04 MPa, the fermentation is 24-32 h, when the enzyme activity of the cellulase is 80-100U/ml, the enzyme activity of the acid pectinase is 3000-4000U/ml, and the enzyme activity of the saccharomyces cerevisiae is 2 multiplied by 107～5×107CFU/ml, Bacillus subtilis 4X 108～6×108When the concentration of the lactobacillus plantarum is CFU/ml, inoculating lactobacillus plantarum seed liquid accounting for 10% v/v of the total volume of the culture medium, and standing and culturing for 24-36 h in an anoxic manner at the temperature of 35-37 ℃ when the saccharomyces cerevisiae is 5 multiplied by 107～1.0×108CFU/ml, Lactobacillus plantarum 2X 108～5×108CFU/ml, Bacillus subtilis 2X 109～5×109CFU/ml, pH3.5-4.0, to obtain the microbial deodorant.
5. The method of preparing a microbial deodorant according to claim 4, further comprising: after the microbial deodorant is prepared, acid lipase, acid protease and low-temperature amylase are added according to enzyme activities, wherein the enzyme activities are respectively 1000-2000U/ml of acid lipase, 1000-2000U/ml of acid protease and 28-35U/ml of low-temperature amylase.
6. The method for using the microbial deodorant is characterized by providing the microbial deodorant prepared by the preparation method of the microbial deodorant according to any one of claims 1-5, wherein the microbial deodorant is used after being diluted by 20-30 times with water.
7. The use method of the microbial deodorant according to claim 6, wherein the microbial deodorant is diluted with water and sprayed in an amount of 5L per cubic meter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010881585.8A CN111961628A (en) | 2020-08-28 | 2020-08-28 | Preparation method and application method of microbial deodorant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010881585.8A CN111961628A (en) | 2020-08-28 | 2020-08-28 | Preparation method and application method of microbial deodorant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111961628A true CN111961628A (en) | 2020-11-20 |
Family
ID=73400547
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010881585.8A Pending CN111961628A (en) | 2020-08-28 | 2020-08-28 | Preparation method and application method of microbial deodorant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111961628A (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258796A (en) * | 2011-06-28 | 2011-11-30 | 黄小娃 | Microbial deodorant and preparation method thereof |
| CN102327630A (en) * | 2011-09-15 | 2012-01-25 | 湖南省微生物研究所 | Microbial strain and deodorant for municipal landfill, and preparation and application method thereof |
| CN102813949A (en) * | 2012-08-27 | 2012-12-12 | 宜宾市万华生物环保有限公司 | Microbial environment protectant |
| CN104388363A (en) * | 2014-12-09 | 2015-03-04 | 山东苏柯汉生物工程股份有限公司 | Compound bacteria for organic refuse deodorization and reduction and preparation method thereof |
| CN105107001A (en) * | 2015-08-29 | 2015-12-02 | 湖南普泰尔微科环保科技有限公司 | Preparation method of efficient microorganism deodorant |
| CN106520627A (en) * | 2016-12-07 | 2017-03-22 | 盐城复华环保产业开发有限公司 | Microbial sludge deodorizer and preparation method thereof |
| CN106731775A (en) * | 2017-01-22 | 2017-05-31 | 广州市佰好惠医疗科技有限公司 | A kind of expansion type compound biological enzyme deodorant and preparation method and application |
| CN108246094A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of composite highly effective liquid bio deodorant and preparation method thereof |
| CN109045999A (en) * | 2018-08-21 | 2018-12-21 | 南京创灵环保科技有限公司 | Compound biological enzyme deodorant and preparation method thereof |
| CN109985511A (en) * | 2019-05-11 | 2019-07-09 | 湖南科美洁环保科技有限公司 | The preparation method and application method of garbage disposal deodorant |
| CN110343649A (en) * | 2019-08-23 | 2019-10-18 | 浙江中益生物环保科技有限公司 | A kind of microbial deoderizer and its application |
| CN110591983A (en) * | 2019-10-30 | 2019-12-20 | 陕西天仁雪农业科技有限公司 | Biological deodorant based on compound microorganisms and preparation method thereof |
| CN110893318A (en) * | 2019-12-07 | 2020-03-20 | 湖南科美洁环保科技有限公司 | Preparation method and use method of public toilet microbial deodorant |
-
2020
- 2020-08-28 CN CN202010881585.8A patent/CN111961628A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258796A (en) * | 2011-06-28 | 2011-11-30 | 黄小娃 | Microbial deodorant and preparation method thereof |
| CN102327630A (en) * | 2011-09-15 | 2012-01-25 | 湖南省微生物研究所 | Microbial strain and deodorant for municipal landfill, and preparation and application method thereof |
| CN102813949A (en) * | 2012-08-27 | 2012-12-12 | 宜宾市万华生物环保有限公司 | Microbial environment protectant |
| CN104388363A (en) * | 2014-12-09 | 2015-03-04 | 山东苏柯汉生物工程股份有限公司 | Compound bacteria for organic refuse deodorization and reduction and preparation method thereof |
| CN105107001A (en) * | 2015-08-29 | 2015-12-02 | 湖南普泰尔微科环保科技有限公司 | Preparation method of efficient microorganism deodorant |
| CN106520627A (en) * | 2016-12-07 | 2017-03-22 | 盐城复华环保产业开发有限公司 | Microbial sludge deodorizer and preparation method thereof |
| CN106731775A (en) * | 2017-01-22 | 2017-05-31 | 广州市佰好惠医疗科技有限公司 | A kind of expansion type compound biological enzyme deodorant and preparation method and application |
| CN108246094A (en) * | 2017-12-29 | 2018-07-06 | 广州广牧丰生物技术有限公司 | A kind of composite highly effective liquid bio deodorant and preparation method thereof |
| CN109045999A (en) * | 2018-08-21 | 2018-12-21 | 南京创灵环保科技有限公司 | Compound biological enzyme deodorant and preparation method thereof |
| CN109985511A (en) * | 2019-05-11 | 2019-07-09 | 湖南科美洁环保科技有限公司 | The preparation method and application method of garbage disposal deodorant |
| CN110343649A (en) * | 2019-08-23 | 2019-10-18 | 浙江中益生物环保科技有限公司 | A kind of microbial deoderizer and its application |
| CN110591983A (en) * | 2019-10-30 | 2019-12-20 | 陕西天仁雪农业科技有限公司 | Biological deodorant based on compound microorganisms and preparation method thereof |
| CN110893318A (en) * | 2019-12-07 | 2020-03-20 | 湖南科美洁环保科技有限公司 | Preparation method and use method of public toilet microbial deodorant |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xu et al. | Fermentation of swine wastewater-derived duckweed for biohydrogen production | |
| CN102327630B (en) | Microbial strain and deodorant for municipal landfill, and preparation and application method thereof | |
| CN101580412B (en) | Low-temperature aerobic composting method for organic solid waste | |
| CN106811438B (en) | Straw degradation acidification microbial inoculum and preparation method thereof | |
| CN1548404A (en) | Production process of microbial organic composite fertilizer | |
| CN105036853A (en) | Method for preparing bio-organic fertilizer by utilizing chicken manure fermentation | |
| CN114875105A (en) | Biomass-based efficient carbon source and preparation method thereof | |
| CN105803004A (en) | Method for low-temperature fermentation of biogas from agricultural waste | |
| CN102334589B (en) | Method for preparing fermented feed from waste bacillus fermentation liquor | |
| CN104862259A (en) | Complex microbial agent for high-organic-load and medium-temperature biogas fermentation as well as preparation method and application thereof | |
| CN105779328A (en) | Clostridium thermocellum culture method | |
| CN101919488A (en) | Method for producing bioactive feed by utilizing yellow seriflux and waste molasses through fermentation | |
| CN111961628A (en) | Preparation method and application method of microbial deodorant | |
| CN103555693A (en) | Culture method for improving activity of clostridium thermoceuum cellulase | |
| CN101475909B (en) | Method for cultivating halophilic bacteria and method for treating salting wastewater by using halophilic bacteria cultivated thereby | |
| CN101177695B (en) | High-concentration alcoholic fermentation method | |
| Boopathy | Inoculum source for anaerobic fermentation of coffee pulp | |
| CN108421402A (en) | A kind of production method of charcoal base biology composite deodurizing material | |
| CN110452852B (en) | Farmer market biomass waste fermentation inoculant and preparation method thereof | |
| CN1470481A (en) | Municipal house refuse termenting method | |
| CN103468618B (en) | Cellulose-degrading bacteria with phosphate-dissolving capability and application thereof | |
| CN102766656A (en) | Method for cheaply preparing microbial flocculant by utilizing bagasse | |
| CN101805709A (en) | Bacterial strain yielding catalase and method of fermenting and yielding enzyme by using bacterial strain and taking citric acid as carbon source | |
| CN114250250B (en) | Method for promoting anaerobic fermentation of straw to produce methane by combining ethanol with biochar and application | |
| CN103540573B (en) | A kind of lignoenzyme and production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20201120 |
|
| WD01 | Invention patent application deemed withdrawn after publication |