CN110591983A - Biological deodorant based on compound microorganisms and preparation method thereof - Google Patents
Biological deodorant based on compound microorganisms and preparation method thereof Download PDFInfo
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- CN110591983A CN110591983A CN201911041021.7A CN201911041021A CN110591983A CN 110591983 A CN110591983 A CN 110591983A CN 201911041021 A CN201911041021 A CN 201911041021A CN 110591983 A CN110591983 A CN 110591983A
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- 239000002781 deodorant agent Substances 0.000 title claims abstract description 31
- 150000001875 compounds Chemical class 0.000 title claims abstract description 26
- 244000005700 microbiome Species 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 241000222178 Candida tropicalis Species 0.000 claims abstract description 16
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 14
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 14
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 14
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000011550 stock solution Substances 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 239000002131 composite material Substances 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
- 239000002068 microbial inoculum Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims description 6
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 238000004332 deodorization Methods 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 235000021552 granulated sugar Nutrition 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229930014456 matrine Natural products 0.000 claims description 6
- 235000013379 molasses Nutrition 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 229920001184 polypeptide Polymers 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 6
- 238000000354 decomposition reaction Methods 0.000 abstract description 4
- 229910021529 ammonia Inorganic materials 0.000 abstract description 3
- QMMFVYPAHWMCMS-UHFFFAOYSA-N Dimethyl sulfide Chemical compound CSC QMMFVYPAHWMCMS-UHFFFAOYSA-N 0.000 abstract 3
- 230000000694 effects Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000001877 deodorizing effect Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 241000407778 Bacillus subtilis TO-A Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004181 pedogenesis Methods 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B1/00—Dumping solid waste
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental & Geological Engineering (AREA)
- Mycology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Biomedical Technology (AREA)
- Hydrology & Water Resources (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention relates to a biological deodorant based on compound microorganisms and a preparation method thereof, wherein the biological deodorant comprises candida tropicalis, lactobacillus plantarum and bacillus subtilis, and the mixing ratio is (5-30): (40-85): (10-30). The invention can accelerate the fermentation and decomposition of organic matters in the kitchen and household garbage, reduce the volume of the accumulated garbage in a short time, and eliminate odor sources such as ammonia, dimethyl sulfide and the like generated by the garbage, thereby reducing the influence of the kitchen and household garbage on the environment.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and particularly relates to a biological deodorant based on compound microorganisms and a preparation method thereof.
Background
The annual clearing volume of domestic kitchen and household garbage in cities in China is about 1.4 hundred million tons, and more than 70 percent of the domestic kitchen and household garbage are transported to a landfill for disposal except for small part of incineration, composting or recycling. Kitchen and household garbage recycling stations are mostly built around living areas, and because organic matters in the kitchen and household garbage recycling stations are decomposed, odor pollution is inevitably generated, and the living environment of residents is influenced. In addition, in the processes of storing, loading and unloading the household garbage and the kitchen garbage, tiling, compacting and the like in a landfill, the external release of malodorous gas cannot be avoided, and the influence on the surrounding human environment can be avoided. Mainly comprises sulfur-containing compounds, nitrogen-containing compounds, halogens and derivatives, hydrocarbons, aromatic hydrocarbons and oxygen-containing organic matters. The odor can seriously harm the health of residents and can generate secondary pollution.
Disclosure of Invention
The invention aims to provide a biological deodorant based on compound microorganisms and a preparation method thereof, which effectively eliminate odor, reduce environmental pollution and improve the living environment of people by degrading odor source substances.
The technical scheme adopted by the invention is as follows:
the biological deodorant based on the compound microorganism is characterized in that:
the biological deodorant comprises candida tropicalis, lactobacillus plantarum and bacillus subtilis.
The mixing ratio of the candida tropicalis, the lactobacillus plantarum and the bacillus subtilis is (5-30): (40-85): (10-30).
The preparation method of the biological deodorant based on the compound microorganism is characterized by comprising the following steps:
the method comprises the following steps:
the first step is as follows: inoculating 5-30% of candida tropicalis, 40-85% of lactobacillus plantarum and 10-30% of bacillus subtilis into a culture medium according to the weight ratio of (1-10) to (99-90), and culturing at 28-35 ℃ for 36-56 hours to obtain a composite microbial inoculum stock solution;
the second step is that: and (3) putting fermentation stock solution consisting of 20-30% of the complex microbial inoculum stock solution obtained in the first step, 25% of molasses, 2-10% of complex plant solution and the balance of water in a closed tank according to the weight ratio, and fermenting at 30 ℃ for 10-20 days to obtain the deodorant.
In the first step, the culture medium is composed of 0.5-0.8% of soybean polypeptide, 0.1-0.3% of brown granulated sugar, 1.0% of glucose, 0.5% of monopotassium phosphate, 0.05% of magnesium sulfate and the balance of water by weight, and the pH value is 7.2.
In the second step, the compound plant solution is prepared by adding 5.0 parts by weight of herba Eragrostidis and 5.0 parts by weight of folium Artemisiae Argyi into 100 parts by weight of water, maintaining at 80-100 deg.C for 5-30 minutes, and adding 2.0% by weight of matrine.
The application of the biological deodorant based on the compound microorganism in the aspect of deodorizing garbage and percolate.
The invention has the following advantages:
1. the fermentation and decomposition of organic matters in the kitchen and household garbage are accelerated, and the volume of the kitchen and household garbage accumulated is reduced in a short time, so that the quantity of the total kitchen and household garbage accumulated quantity is increased.
2. Inhibit the growth of putrefying bacteria and prevent the generation of diseases. Improves the decomposition way of organic matters, reduces the release amount of ammonia and hydrogen sulfide and the generation of ammonia substances, and simultaneously reduces the methane amount generated by the decomposition of bacteria, thereby achieving the aim of deodorization.
3. The invention can improve the water quality of the percolating water, greatly reduce various pollution components in the percolating water and promote the cycle process of soil formation and regeneration of the refuse landfill area.
Detailed Description
The present invention will be described in detail with reference to specific embodiments.
The invention relates to a biological deodorant based on compound microorganisms, which comprises candida tropicalis, lactobacillus plantarum and bacillus subtilis, wherein the mixing ratio is (5-30): (40-85): (10-30).
The preparation method of the biological deodorant based on the compound microorganism comprises the following steps:
the first step is as follows: inoculating 5-30% of candida tropicalis, 40-85% of lactobacillus plantarum and 10-30% of bacillus subtilis into a culture medium according to the weight ratio of (1-10) to (99-90), and culturing at 28-35 ℃ for 36-56 hours to obtain a composite microbial inoculum stock solution;
the second step is that: and (3) putting fermentation stock solution consisting of 20-30% of the complex microbial inoculum stock solution obtained in the first step, 25% of molasses, 2-10% of complex plant solution and the balance of water in a closed tank according to the weight ratio, and fermenting at 30 ℃ for 10-20 days to obtain the deodorant.
In the first step, the culture medium is composed of 0.5-0.8% of soybean polypeptide, 0.1-0.3% of brown granulated sugar, 1.0% of glucose, 0.5% of monopotassium phosphate, 0.05% of magnesium sulfate and the balance of water by weight, and the pH value is 7.2.
In the second step, the compound plant solution is prepared by adding 5.0 parts by weight of herba Eragrostidis and 5.0 parts by weight of folium Artemisiae Argyi into 100 parts by weight of water, maintaining at 80-100 deg.C for 5-30 minutes, and adding 2.0% by weight of matrine.
Bacillus subtilis (A) and (B) as described aboveBacillus subtilis) Accession number ACCC 01031, Candida tropicalis (C.tropicalis: (C.tropicalis))Candida tropicalis) Accession number ACCC 20006, Lactobacillus plantarum: (Lactobacillus plantarum) The number ACCC 11016 can be obtained from China agricultural microbial strain preservation management center.
The biological deodorant based on the compound microorganisms can be used for biological deodorization of garbage and leachate, and can effectively decompose organic matters, harmful pollutants, odor and the like in the kitchen, the household garbage and the leachate based on the comprehensive effect of the compound microorganisms of the biological deodorant, so that the effects of deodorization and harmless treatment are achieved. Wherein the microorganism converts H2Reduction of S to S and H2Thereby achieving the aim of deodorization; through the anaerobic fermentation of the microorganisms, the organic macromolecules in the percolate are decomposed into micromolecular acids and soluble substances, and the microorganisms are propagated and oxidized into carbon dioxide and water to complete the biodegradation process. The compound plant extract can inhibit the activity and reproduction of pathogenic bacteria and sclerotinia sclerotiorum, so that harmful bacteria can disappear gradually.
Example 1
A method for preparing a biological deodorant by using compound microorganisms comprises the following specific steps:
the first step is as follows: inoculating a composite strain consisting of 30% of candida tropicalis, 50% of lactobacillus plantarum and 20% of bacillus subtilis into a culture medium according to the weight ratio of 5:95, and culturing at 30 ℃ for 48 hours to obtain a composite microbial inoculum stock solution;
the culture medium comprises 0.7% of soybean polypeptide, 0.2% of brown granulated sugar, 1.0% of glucose, 0.5% of monopotassium phosphate, 0.05% of magnesium sulfate and the balance of water by weight, and the pH value is 7.2;
the second step is that: and (3) putting a fermentation stock solution consisting of 28% of the complex microbial inoculum stock solution obtained in the first step, 25% of molasses, 6% of the complex plant solution and the balance of water in a closed tank according to the weight ratio, and fermenting for 15 days at 30 ℃ to obtain the deodorant.
Furthermore, the compound plant solution is prepared by adding 5.0 parts by weight of herba Eragrostidis and 5.0 parts by weight of folium Artemisiae Argyi into 100 parts by weight of water, keeping at 90 deg.C for 20 minutes, and adding 2.0% by weight of matrine.
Example 2
A method for preparing a biological deodorant by using compound microorganisms comprises the following specific steps:
the first step is as follows: inoculating a composite strain consisting of 5% of candida tropicalis, 85% of lactobacillus plantarum and 10% of bacillus subtilis into a culture medium according to the weight ratio of 1: 99, and culturing at 35 ℃ for 36 hours to obtain a composite microbial inoculum stock solution;
the culture medium comprises 0.5% of soybean polypeptide, 0.1% of brown granulated sugar, 1.0% of glucose, 0.5% of monopotassium phosphate, 0.05% of magnesium sulfate and the balance of water by weight, and the pH value is 7.2;
the second step is that: and (3) putting a fermentation stock solution consisting of 20% of the complex microbial inoculum stock solution obtained in the first step, 25% of molasses, 2% of a compound plant solution and the balance of water in a closed tank according to the weight ratio, and fermenting for 10 days at 30 ℃ to obtain the deodorant.
Furthermore, the compound plant solution is prepared by adding 5.0 parts by weight of herba Eragrostidis and 5.0 parts by weight of folium Artemisiae Argyi into 100 parts by weight of water, keeping at 100 deg.C for 5 minutes, and adding 2.0% by weight of matrine.
Example 3
A method for preparing a biological deodorant by using compound microorganisms comprises the following specific steps:
the first step is as follows: inoculating 30% of candida tropicalis, 40% of lactobacillus plantarum and 30% of bacillus subtilis to a culture medium according to the weight ratio of 10:90, and culturing at 28 ℃ for 56 hours to obtain a composite microbial inoculum stock solution;
the culture medium comprises 0.8% of soybean polypeptide, 0.3% of brown granulated sugar, 1.0% of glucose, 0.5% of monopotassium phosphate, 0.05% of magnesium sulfate and the balance of water by weight, and the pH value is 7.2;
the second step is that: and (3) putting a fermentation stock solution consisting of 20% of the complex microbial inoculum stock solution obtained in the first step, 25% of molasses, 10% of compound plant solution and the balance of water in a closed tank according to the weight ratio, and fermenting for 20 days at 30 ℃ to obtain the deodorant.
Furthermore, the compound plant solution is prepared by adding 5.0 parts by weight of herba Eragrostidis and 5.0 parts by weight of folium Artemisiae Argyi into 100 parts by weight of water, keeping at 8 ℃ for 30 minutes, and then adding 2.0% by weight of matrine.
Application effects
The product prepared by the invention is subjected to a deodorizing effect detection test according to the technical requirement of CJ/T516-2017 household garbage deodorant as follows:
the deodorizer prepared by the invention is sprayed when kitchen and household garbage are collected or sprayed after the kitchen and household garbage are diluted according to the proportion of 1:300 when being transported to a landfill. The practical application has obvious deodorization effect, and the kitchen and household garbage after being sprayed has no original odor; in the domestic garbage landfill, the percolation water of the domestic garbage has no odor.
The invention is not limited to the examples, and any equivalent changes to the technical solution of the invention by a person skilled in the art after reading the description of the invention are covered by the claims of the invention.
Claims (6)
1. The biological deodorant based on the compound microorganism is characterized in that:
the biological deodorant comprises candida tropicalis, lactobacillus plantarum and bacillus subtilis.
2. The complex microorganism-based biological deodorant according to claim 1, characterized in that:
the mixing ratio of the candida tropicalis, the lactobacillus plantarum and the bacillus subtilis is (5-30): (40-85): (10-30).
3. The method for preparing a composite microorganism-based biological deodorant according to claim 1 or 2, wherein:
the method comprises the following steps:
the first step is as follows: inoculating 5-30% of candida tropicalis, 40-85% of lactobacillus plantarum and 10-30% of bacillus subtilis into a culture medium according to the weight ratio of (1-10) to (99-90), and culturing at 28-35 ℃ for 36-56 hours to obtain a composite microbial inoculum stock solution;
the second step is that: and (3) putting fermentation stock solution consisting of 20-30% of the complex microbial inoculum stock solution obtained in the first step, 25% of molasses, 2-10% of complex plant solution and the balance of water in a closed tank according to the weight ratio, and fermenting at 30 ℃ for 10-20 days to obtain the deodorant.
4. The method for preparing a biological deodorant based on composite microorganisms according to claim 3, characterized in that:
in the first step, the culture medium is composed of 0.5-0.8% of soybean polypeptide, 0.1-0.3% of brown granulated sugar, 1.0% of glucose, 0.5% of monopotassium phosphate, 0.05% of magnesium sulfate and the balance of water by weight, and the pH value is 7.2.
5. The method for preparing a biological deodorant based on composite microorganisms according to claim 4, wherein:
in the second step, the compound plant solution is prepared by adding 5.0 parts by weight of herba Eragrostidis and 5.0 parts by weight of folium Artemisiae Argyi into 100 parts by weight of water, maintaining at 80-100 deg.C for 5-30 minutes, and adding 2.0% by weight of matrine.
6. Use of a composite microorganism-based biological deodorant according to claim 1 or 2 for the deodorization of landfill and leachate.
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| CN201911041021.7A CN110591983A (en) | 2019-10-30 | 2019-10-30 | Biological deodorant based on compound microorganisms and preparation method thereof |
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| CN201911041021.7A CN110591983A (en) | 2019-10-30 | 2019-10-30 | Biological deodorant based on compound microorganisms and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961628A (en) * | 2020-08-28 | 2020-11-20 | 湖南科美洁环保科技有限公司 | Preparation method and application method of microbial deodorant |
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| CN101356950A (en) * | 2008-09-24 | 2009-02-04 | 东北农业大学 | Preparation method of composite bacteria fermentation bed |
| CN101671643A (en) * | 2009-09-27 | 2010-03-17 | 上海创博生态工程有限公司 | Method for preparing deodorizer by composite microorganism |
| CN109806717A (en) * | 2019-03-15 | 2019-05-28 | 福建红天然环保科技有限公司 | Plant deodorant and its preparation process |
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- 2019-10-30 CN CN201911041021.7A patent/CN110591983A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101356950A (en) * | 2008-09-24 | 2009-02-04 | 东北农业大学 | Preparation method of composite bacteria fermentation bed |
| CN101671643A (en) * | 2009-09-27 | 2010-03-17 | 上海创博生态工程有限公司 | Method for preparing deodorizer by composite microorganism |
| CN109806717A (en) * | 2019-03-15 | 2019-05-28 | 福建红天然环保科技有限公司 | Plant deodorant and its preparation process |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111961628A (en) * | 2020-08-28 | 2020-11-20 | 湖南科美洁环保科技有限公司 | Preparation method and application method of microbial deodorant |
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Application publication date: 20191220 |