CN109293000A - A kind of suspend mode storage application method of suspended biological active filler - Google Patents
A kind of suspend mode storage application method of suspended biological active filler Download PDFInfo
- Publication number
- CN109293000A CN109293000A CN201811215889.XA CN201811215889A CN109293000A CN 109293000 A CN109293000 A CN 109293000A CN 201811215889 A CN201811215889 A CN 201811215889A CN 109293000 A CN109293000 A CN 109293000A
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- Prior art keywords
- suspend mode
- biological active
- active filler
- suspended biological
- room temperature
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of suspend mode of suspended biological active filler to store application method, and dry be placed in storage bag under suspended biological active filler dehydration, room temperature or the low condition grown will be trained in water body and is saved in a low temperature of 4 DEG C or less；When using can under room temperature or cold transport activity recovery in water body at normal temperature.A kind of suspend mode store method after floating stuffing immobilized nitrifying bacteria enrichment.This method is directly by the nitrobacteria of the high concentration, high activity that are enriched on floating stuffing, vacuum deaeration dry by low-speed centrifugal, 4 DEG C of low temperature, long-term preservation.And for the immobilized nitrifying bacteria after suspend mode because of part aging or death, slow growth, again cultivation period be long, biochemical system and heterotroph compete it is not dominant, be easy to run off the deficiencies of, the disadvantage for removing the decline of ammonia nitrogen ability, proposes the method for fast quick-recovery nitrification activity function.
Description
Technical field
The present invention relates to environment water-treatment technology field, especially industrial circulating water cultivatings and cultivating wastewater purification technology
Field, more particularly to the suspend mode store method of immobilized nitrifying bacteria, the method for fast quick-recovery nitrification activity function.
Background technique
(suspension) filler is mostly used to load when aerobic oxygen or even anaerobic organism water process (or even including film biological treatment),
Filler load is to guarantee the biological reactivity of water process and the important means and method of efficiency, although existing active microbial inoculum is adopted
Suspend mode storage transport is carried out with liquid or the mode of pulvis and activity recovery uses, but and is not easy to load or hang on filler
On film, especially filler load or biofilm activity be can be in the permanent process cycle of the comparison of culture-cycle or water at water
Manage the guarantee of efficiency.Active microbial inoculum can only largely consume active microbial inoculum when using by the way of liquid or pulvis, and make into
It is much higher than the use condition of load or biofilm in active filler in sheet, especially such use manually more is taken, water process
Effect is not as good as active filler.
Nitrogen is one of the main reason for causing water eutrophication, and breeding wastewater is discharged into before natural water must be into
Row denitrogenation processing.Biological denitrificaion is using most common denitrogenation technology, and principle is the nitrification first by nitrobacteria
Mineralized nitrogen nitrite nitrogen in waste water and then nitrate nitrogen will be converted to, then by the denitrification of denitrifying bacterium by nitre
State nitrogen is converted into nitrogen, escapes and removes from waste water, this is the most common breeding way.Another kind is exactly more advanced circulation
Water breeding way, the nitrogen in breeding process complete circulation by nitrification, anti-nitration reaction and reach zero-emission.The premise of the two is all
It is to ensure that being normally carried out for nitration reaction.Nitrification must be completed by nitrobacteria, and nitrobacteria belongs to chemautotrophy
Bacterium, generation time is longer, harsher to living environment requirement, and cultivation water or the variation water quality for discharging water easily make nitrobacteria
It is set to lose nitrification function at impact, nitrification function is difficult to spontaneous recovery once losing.There are also using work for traditional method
Property mud adds the store method of nutrition and restores its function, but because existing method technology is unstable, inconvenient to use, resume speed
Slowly, therefore there is biggish application limitation.
Summary of the invention
Object of the present invention is to, propose a kind of suspended biological active filler suspend mode storage method and fast quick-recovery nitrification activity
The method of function, present invention employs no nutrition, no moisture (drying), no oxygen or hypoxemia (to vacuumize) life of filler supported active
The Cryopreservation of object thallus makes the application technology of active bio thallus long period in a dormant state.
The technical solution adopted in the present invention: a kind of suspend mode storage application method of suspended biological active filler, it will be in water
Trained in body under suspended biological active filler dehydration, room temperature or the low condition grown it is dry be placed in storage bag it is below low at 4 DEG C
Temperature is lower to be saved；When using can under room temperature or cold transport activity recovery in water body at normal temperature.Especially storage bag into
Row vacuumizes preferably.
The present invention uses the necessary condition controlled in the bacterial growths metabolic processes such as nitrification, i.e. nutrition, oxygen, thermophilic,
A large amount of active nitrobacterias are allowed to rapidly enter dormant state and save.Centrifugal drying is carried out by 800 revolving speeds/minute respectively
(be free of nutrition) vacuumizes (anaerobic i.e. cannot be metabolic), quick 4 DEG C of low temperature (basic metabolism is extremely low) it is suitable
Sequence makes the bacteriums such as nitrification enter dormant state.
The utility model has the advantages that the present invention solve existing microbial inoculum active microbial inoculum load filler on preservation, storage transport and it is extensive
Active technical problem, the method for the microbial activities function such as especially fast quick-recovery nitrification；Present invention employs a kind of non-
Traditional preservation method, and using no additional nutrient, no moisture, no oxygen athermobiosis store method, solve micro- lifes such as nitrification
Object bacterium and carrier mutual survival, simultaneously holding time long period (more than half a year activity is unaffected), solve to save activity compared with
The problem that low and fast quick-recovery nitrification activity is relatively slow and transport is inconvenient, the operating cost of the method for the present invention is low, is suitable for environmental protection
Water process is especially adapted for use in the preservation and transport of the active floating stuffing of aquaculture water process.Preferably cold chain when transport
Transport or be lower than 20 DEG C of temperature when it is more preferable, can also guarantee bring back to life activity, convenient for the life using breeding pool of raiser
Object water process.The present invention is especially by the suspend mode store method after the enrichment of floating stuffing immobilized nitrifying bacteria.This method is direct
By the bacterium of the high concentration, high activity that are enriched on floating stuffing, dry by low-speed centrifugal, vacuum deaeration, 4 DEG C or less low
Temperature, long-term preservation.And for the immobilization bacterium after suspend mode because of part aging or death, slow growth, again cultivation period be long,
Biochemical system and heterotroph competition is dominant, the deficiencies of being easy to run off, the disadvantage of removal ammonia nitrogen ability decline proposes quickly extensive
The method of multiple nitrification activity function.
Specific embodiment
Present invention process process, culture medium prescription and embodiment: the suspend mode storage of suspended biological active filler of the present invention
Application method will train drying under suspended biological active filler dehydration, room temperature or the cryogenic conditions grown in water body and be placed on preservation
In bag, long period in a low temperature of 4 DEG C or less is especially needed to save, in room temperature or cold chain transportation；It can be when using
Activity recovery in water body under room temperature.Especially vacuumized preferably in storage bag.Detailed process:
1, suspended biological active filler dehydration turns especially 800 revolving speeds/minute low-speed centrifugal using 500-1500, to maturation
The floras such as activity nitrification and its carrier (active floating stuffing) are centrifuged 3-8 minutes, it is ensured that moisture content is low；It is dry that freezing can be carried out again
Dry or dry air at room temperature is dried 3-5 minutes.
2, will centrifugation or again plus the nitrifying bacteria community that was freeze-dried and its carrier (floating stuffing) be put into vacuum packaging bag into
Row vacuumizes, and is allowed to dry drying prescription and oxygen indicator, in packaging bag to determine nitrifying bacteria community in a dormant state；
3, then nitrifying bacteria community and its carrier (floating stuffing) that vacuum packet installs are put into 4 DEG C or less low temperature refrigerators,
It can save spare in 3-6 months；It can also be saved under 0 to minus 18 DEG C or less cryogenic conditions.
4, it needs in use, water body side is transported, by the nitrifying bacteria community and its carrier (floating stuffing) in vacuum packaging bag
After opening the package, active floating stuffing is put into be prepared culture medium and (can use different trainings according to different strains in advance
Support base) container in carries out 24-96 hour active spontaneous recoveries, especially restore biological (dropping ammonia) ability.Culture medium presses it
Physical state can be divided into fluid nutrient medium or semisolid culturemedium, the common nutritious broth bouillon of bacteria culture media etc.；Often
Unwrapping wire bacterium culture medium is No. 1 culture medium of Gao Shi；Common microzyme culture medium has potato sucrose culture medium and brewer's wort
Culture medium；Common mould medium has potato sucrose culture medium, bean sprout juice glucose (or sucrose) agar medium and examines
Family name's culture medium etc., it is necessary to which ingredient is provided with.
4, the microbial activity carrier after culture medium culture is added in water body.
Claims (6)
1. the suspend mode of suspended biological active filler a kind of stores application method, characterized in that by water body having of growing of training live
Property suspended biological active filler dehydration, it is dry after be placed in storage bag in a low temperature of 4 DEG C or less under room temperature or cryogenic conditions
It saves.
2. according to the method described in claim 1, it is characterized in that, storage bag is vacuumized.
3. according to the method described in claim 1, it is characterized in that, centrifugal drying is turned using 500-1500 per minute.
4. according to the method described in claim 3, it is characterized in that, then the air at room temperature for being freeze-dried or being dried is dried
3-5 minutes.
5. according to the method described in claim 3, it is characterized in that, use when can be in room temperature or cold chain transportation.
6. according to the method described in claim 3, it is characterized in that, at normal temperature through 24-96 hours activity recoveries of fluid nutrient medium
After enter back into water body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811215889.XA CN109293000A (en) | 2018-10-18 | 2018-10-18 | A kind of suspend mode storage application method of suspended biological active filler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811215889.XA CN109293000A (en) | 2018-10-18 | 2018-10-18 | A kind of suspend mode storage application method of suspended biological active filler |
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| CN109293000A true CN109293000A (en) | 2019-02-01 |
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| CN201811215889.XA Pending CN109293000A (en) | 2018-10-18 | 2018-10-18 | A kind of suspend mode storage application method of suspended biological active filler |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565239A (en) * | 2009-05-23 | 2009-10-28 | 无锡绿水之源生物科技有限公司 | Method for preparing composite heterotrophic nitrobacteria water quality modifying solid powder or granule |
| CN101812440A (en) * | 2010-01-22 | 2010-08-25 | 北京桑德环保集团有限公司 | Nitrifying bacteria vector for degrading ammonia nitrogen and preparation method thereof |
| CN101919879A (en) * | 2010-08-06 | 2010-12-22 | 淮阴工学院 | Method for preparing probiotics preparation by taking attapulgite as carrier |
| CN106520627A (en) * | 2016-12-07 | 2017-03-22 | 盐城复华环保产业开发有限公司 | Microbial sludge deodorant and preparation method thereof |
| CN106754547A (en) * | 2017-01-17 | 2017-05-31 | 广州市广深环保科技有限公司 | A kind of high-efficiency multi-function sewage activation of microorganism microbial inoculum and its application |
| JP2017177027A (en) * | 2016-03-31 | 2017-10-05 | 株式会社システック | Iron bacteria carrier and preparation method thereof and ph control method of basic solution using the same |
-
2018
- 2018-10-18 CN CN201811215889.XA patent/CN109293000A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565239A (en) * | 2009-05-23 | 2009-10-28 | 无锡绿水之源生物科技有限公司 | Method for preparing composite heterotrophic nitrobacteria water quality modifying solid powder or granule |
| CN101812440A (en) * | 2010-01-22 | 2010-08-25 | 北京桑德环保集团有限公司 | Nitrifying bacteria vector for degrading ammonia nitrogen and preparation method thereof |
| CN101919879A (en) * | 2010-08-06 | 2010-12-22 | 淮阴工学院 | Method for preparing probiotics preparation by taking attapulgite as carrier |
| JP2017177027A (en) * | 2016-03-31 | 2017-10-05 | 株式会社システック | Iron bacteria carrier and preparation method thereof and ph control method of basic solution using the same |
| CN106520627A (en) * | 2016-12-07 | 2017-03-22 | 盐城复华环保产业开发有限公司 | Microbial sludge deodorant and preparation method thereof |
| CN106754547A (en) * | 2017-01-17 | 2017-05-31 | 广州市广深环保科技有限公司 | A kind of high-efficiency multi-function sewage activation of microorganism microbial inoculum and its application |
Non-Patent Citations (1)
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| 张帆等: "厌氧氨氧化菌的真空冷冻干燥保藏及复壮性能", 《环境科学与技术》 * |
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Application publication date: 20190201 |