CN107653197A - A kind of preparation method of microorganism formulation and its application in leather waste water processing - Google Patents
A kind of preparation method of microorganism formulation and its application in leather waste water processing Download PDFInfo
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- CN107653197A CN107653197A CN201711113270.3A CN201711113270A CN107653197A CN 107653197 A CN107653197 A CN 107653197A CN 201711113270 A CN201711113270 A CN 201711113270A CN 107653197 A CN107653197 A CN 107653197A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/22—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof
- C02F2103/24—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof from tanneries
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Abstract
A kind of preparation method of microorganism formulation and its application in leather waste water processing, the present invention relates to Industrial Waste Water Treatments, and to provide a kind of microorganism formulation of leather sewage processing, described microorganism formulation includes following effective viable bacteria：Saccharomyces cerevisiae 106‑107CFU/mL, candida utili 106‑108CFU/mL, lactobacillus fermenti 106‑108CFU/mL, Lactobacillus casei 106‑108CFU/mL；Photosynthetic bacteria 105‑107CFU/mL, gamboge coccus 105‑107CFU/mL mentioned microorganisms preparation is used to handle leather waste water.The beneficial effects of the present invention are：The biological agent of the present invention is resistant to and sulfide and lipid in efficient-decomposition leather waste water, save the link of part coagulating sedimentation in the original handling process of leather waste water, the growth of the microorganisms such as spoilage organisms can effectively be suppressed while chemical sludge yield is greatly lowered, solve caused malodor problem in wastewater treatment process.
Description
Technical field
The present invention relates to Industrial Waste Water Treatments, it relates in particular to the preparation method of a kind of microorganism formulation and its in skin
Remove from office the application in wastewater treatment.
Background technology
Leather industry reaches 100,000,000 tons to the seriously polluted of environment, the existing leather-making enterprises year waste discharge water in China.Due to making
Removing from office raw material availability in process, than relatively low, a large amount of protein and fat are transferred in waste water, while are added in leather making process
Enter substantial amounts of industrial chemicals：Acid, alkali, salt, vulcanized sodium, lime, surfactant, chrome tanning agent, dyestuff etc., except a part is absorbed
Outer major part pollutes into waste water.Leather-making waste water colourity is deep, foul smell is heavy, suspension is more, pH value is high, content of organics is high,
Complicated component, because waste water interval is discharged, water quality and quantity fluctuation is big, easily corruption；Have in water containing chromium ion and sulfide etc. and poison
Compound needs pre-treatment to remove laggard biochemical system processing.
Leather waste water treatment technology mainly carries out pre-treatment by chemical flocculation at present, and subsequent biological oxidation is handled, most
Handled by high-level oxidation technology and membrane filtration even depth, needed in conventional process to pickling chrome tanning waste water, containing Ca afterwards
(OH)2、Na2S strong basicity depilation liming waste water, containing grease and saponified degreasing waste water shunted and pre-processed, not only located
It is cumbersome to manage complex process, using chemicals such as flocculant, acid in processing procedure, forms a large amount of excess sludges, processing cost is high
Meanwhile plant area is generally with foul odour.Microbial bacterial agent relative specificity on domestic market is poor, although after use
Biochemical system efficiency is improved to a certain extent, but still needs to front end Individual pretreatment, and product processes are cumbersome, cost is high.
Therefore it provides to answer easy-operating sewage treatmentmethod necessary for a kind of letter.
The content of the invention
Mentioned above to solve the problems, such as, the present invention provides a kind of following technical scheme：
A kind of application of microorganism formulation in leather waste water processing, described microorganism formulation include following effective viable bacteria：
Saccharomyces cerevisiae 106-107CFU/mL, candida utili 106-108CFU/mL, lactobacillus fermenti 106-108CFU/mL, cheese breast
Bacillus 106-108CFU/mL；Photosynthetic bacteria 105-107CFU/mL, gamboge coccus 105-107CFU/mL mentioned microorganisms preparation is used
In processing leather waste water.
Further, described microorganism formulation pH value is 3-3.6.
Further, described microorganism formulation living bacteria count >=5 × 108CFU/mL。
Further, the sewage disposal that described microorganism formulation is used for after pig, ox, woolfell and wet blue processing.
Further, in use, molasses and microorganism formulation press 5:6 weight after mixing sudden and violent gas activation 48-72h than feeding intake
Use, inventory is the 1%-1.5% of sewage volume.
A kind of preparation method of microorganism formulation, described preparation method comprise the following steps：Step 1,（1）Lactic acid bacteria
Culture：Lactobacillus fermenti strain and species L. casei are taken out and are inoculated in respectively in lactic acid bacteria culture solution, in incubated
28-32 DEG C of culture in case, then by cultured lactic acid bacteria 28-32 DEG C expand culture, detect pH value after the completion of culture, pH value need to be
Less than 4；（2）Saccharomycete culture：Saccharomyces cerevisiae strain and candida utili strain are taken out and are inoculated in saccharomycete culture respectively
In liquid, the 28-32 DEG C of culture in constant incubator, then cultured 28-32 DEG C of lactic acid bacteria is expanded into culture, examined after the completion of culture
Cell concentration is surveyed, cell concentration need to reach 106More than；(3) photosynthetic bacteria is cultivated：Strain is taken out and is inoculated in photosynthetic bacteria training
In nutrient solution, 28-32 DEG C of culture under illumination anaerobic condition in transparent cell, then by cultured 28-32 DEG C of illumination anaerobism of photosynthetic bacteria
Expand culture, cell concentration is detected after the completion of culture, cell concentration need to reach 105More than；（4）Gamboge coccus is cultivated：By gamboge
Coccus strain takes out and is inoculated in inoculum, the 30-32 DEG C of culture in constant incubator, then by cultured gamboge coccus
30-32 DEG C expands culture, and cell concentration need to reach 105More than；
Step 2, seed tank culture：Culture medium is added in the stainless steel seeding tank with agitating device, culture medium collocation method is such as
Under：Molasses 2wt%, peptone 0.5wt%, yeast extract 0.5wt%, K2HPO40.2wt%, MgSO4•7H2O, remaining is water, is gone out
Room temperature is cooled to after bacterium, takes the strain in step 1 to be inoculated with, inoculative proportion is：Saccharomycete 2-4wt%, lactic acid bacteria 4-6wt%, light
Bacterium 5-10wt% is closed, gamboge coccus 2-4 wt %, remaining is water, and ferment 72-80h under the conditions of 30-32 DEG C, every 1-2 hours
Once, each aeration agitation 1-2h, i.e. stopping when pH value is reduced to 3-3.6, living bacteria count is up to 5 × 10 for stirring8CFU/mL,
Obtain seed liquor；
Step 3：Culture medium is added in the stainless steel fermentation tank with agitating device, culture medium collocation method is as follows：Molasses
2wt%, yeast extract（Yeast extract）0.5wt%, remaining is water, and room temperature, inoculation step are cooled to after sterilizing（2）In seed
Liquid, inoculum concentration 10wt%, ferment 72-80h under the conditions of 30-32 DEG C, every 1-2 hours aeration agitation once, stirs 1- every time
2h, stop when pH value is reduced to 3-3.6.Described yeast extract is commercially available yeast extract.
Further, step（Two）And step（Three）In for aeration culture.
The beneficial effects of the present invention are：A kind of biological agent for leather waste water processing provided by the invention, its energy
Sulfide and lipid caused by the liming depilation being resistant in simultaneously efficient-decomposition leather waste water and degreasing process, save leather waste water
The link of part coagulating sedimentation in original handling process, can effectively suppress spoilage organisms while chemical sludge yield is greatly lowered
Deng the growth of microorganism, solves caused malodor problem in wastewater treatment process.
Brief description of the drawings
Fig. 1 is active pollution index microscopy figure under 10 × eyepiece in embodiment.
Embodiment
This case is described further with reference to embodiment.
A kind of preparation method of microorganism formulation, described preparation method comprise the following steps：
Step 1：（1）Lactic acid bacteria（That is lactobacillus fermenti and Lactobacillus casei）Culture：The strain being stored in cryopreservation tube is taken out
It is inoculated in 200ml lactic acid bacteria culture solutions, 32 DEG C of culture 48h, cultured 200ml lactic acid bacterias are expanded in constant incubator
Culture 32 DEG C of culture 48h, detects pH value, pH value need to be below 4 to 4L after the completion of culture；（2）Saccharomycete（I.e. saccharomyces cerevisiae and
Candida utili）Culture：The strain being stored in cryopreservation tube is taken out and is inoculated in 200ml yeast bacteria culture fluids, in constant temperature
32 DEG C of culture 48h in incubator, cultured 200ml lactic acid bacterias are expanded into culture to 4L, 32 DEG C of culture 24h, after the completion of culture
Cell concentration is detected, cell concentration need to reach 106More than；(3) photosynthetic bacteria culture：The strain being stored in cryopreservation tube is taken out and connect
Kind is in 1L photosynthetic bacteria culture solutions, and the lower 32 DEG C of cultures 96h of illumination anaerobic condition, cultured photosynthetic bacteria is expanded in transparent cell
Cultivate to 10L greatly, 32 DEG C of illumination Anaerobic culturel 96h, cell concentration is detected after the completion of culture, cell concentration need to reach 105More than；
（4）Gamboge coccus is cultivated：Gamboge coccus strain is taken out and is inoculated in inoculum, the 30-32 DEG C of training in constant incubator
Support, then cultured 30-32 DEG C of gamboge coccus is expanded into culture, cell concentration need to reach 105More than.
Step 2, seed tank culture：Culture medium 240L, culture are added in stainless steel seeding tanks of the 300L with agitating device
Base collocation method is as follows：Molasses 2wt%, peptone 0.5wt%, yeast extract 0.5wt%, gamboge coccus 2-4 wt %, remaining is water,
PH value takes cultured strain inoculation in step 1, inoculative proportion is ferment naturally, be cooled to room temperature after 70 DEG C of sterilizing 100min
Female bacterium 2-4%, lactic acid bacteria 4-6%, photosynthetic bacteria 5-10%, surplus are water（Water primarily serves the effect of amplification culture）, in 30-32
Ferment 72-96h under the conditions of DEG C, daily intermittent stirring 1-2h, is to be stirred once every 1-2 hours, stirs 1-2h every time, can also
To be aerated culture by a small margin, pH value is detected after 72h, is stopped when pH value is reduced to 3-3.6, living bacteria count up to 5 ×
108CFU/mL；
Step 3：2.1 tons of culture medium is added in three tons of stainless steel fermentation tanks with agitating device, culture medium collocation method is such as
Under：Molasses 2%, remaining is water, and pH value after 70 DEG C of sterilizing 100min naturally, be cooled to room temperature, inoculation step（2）In seed liquor,
Inoculum concentration 10%, fermented 72-80h under the conditions of 30-32 DEG C, and pH value is detected after daily intermittent stirring 1-2h, 72h, treats pH value reduction
Stop to during 3-3.6.
Microorganism formulation of the present invention is applied to pig, ox, woolfell and wet blue new technology processing leather sewage treatment plant：
Molasses 250kg is added in 10 tons of storage tanks, 300kg strains is added, adds to regulating reservoir and Aerobic Pond, throw after sudden and violent gas activation 48-72h
Dosage is the 1%-0.5% of inflow, in addition to chrome waste liquid needs independent precipitation process, without other chemical treatment technologies, after use
Sludge is greatly reduced, and plant area's stink substantially mitigates.
The microorganism formulation prepared using above-described embodiment is to ox-hide processing waste water aeration test：
Activated sludge concentration is 9000mg/L, SV after adding product aeration30=90%, it is quiet to after 4-5h up to 40-50%, sludge into
Taupe, shown in activated sludge microscopy accompanying drawing 1, it can be seen that low power Microscopic observation, compared in ordinary activated sludge, flco
It is granular to become apparent from, compared with control group, whether described control group and differing only in for experimental group have used this case offer
Microorganism formulation, the dosage of experimental group microorganism formulation is the 0.5% of sewage volume, and water outlet data are as follows：
| Testing index | Enter water COD（mg/L） | Water outlet COD（mg/L） |
| Experimental group | 6500-7500 | 500-550 |
| Control group | 6500-7500 | 750-900 |
Claims (7)
1. application of a kind of microorganism formulation in leather waste water processing, it is characterised in that described microorganism formulation includes
Effective viable bacteria below：Saccharomyces cerevisiae 106-107CFU/mL, candida utili 106-108CFU/mL, lactobacillus fermenti 106-
108CFU/mL, Lactobacillus casei 106-108CFU/mL；Photosynthetic bacteria 105-107CFU/mL, gamboge coccus 105-107CFU/mL,
Mentioned microorganism preparation is used to handle leather waste water.
2. application as claimed in claim 1, it is characterised in that described microorganism formulation pH value is 3-3.6.
3. application as claimed in claim 1, it is characterised in that described microorganism formulation living bacteria count >=5 × 108CFU/
mL。
4. application as claimed in claim 1, it is characterised in that it is wet that described microorganism formulation is used for pig, ox, woolfell and indigo plant
Sewage disposal after skin processing.
5. application as claimed in claim 1, it is characterised in that in use, molasses and microorganism formulation press 5:6 weight is than mixed
Feed intake use after conjunction aeration activation 48-72h, and inventory is the 1%-0.5% of sewage quantity volume.
6. a kind of preparation method of microorganism formulation, it is characterised in that described preparation method comprises the following steps：
Step 1,（1）Lactic acid bacteria is cultivated：Lactobacillus fermenti strain and species L. casei are taken out and are inoculated in lactic acid bacteria respectively
In nutrient solution, the 28-32 DEG C of culture in constant incubator, then cultured 28-32 DEG C of lactic acid bacteria is expanded into culture, culture is completed
After detect pH value, pH value need to be below 4；（2）Saccharomycete culture：Saccharomyces cerevisiae strain and candida utili strain are taken out and divided
It is not inoculated in yeast bacteria culture fluid, the 28-32 DEG C of shaken cultivation in constant incubator, then by cultured lactic acid bacteria 28-32
DEG C expand culture, cell concentration is detected after the completion of culture, cell concentration need to reach 106More than；(3) photosynthetic bacteria is cultivated：By bacterium
Kind of taking-up is inoculated in photosynthetic bacteria culture solution, 28-32 DEG C of culture under illumination anaerobic condition in transparent cell, then by cultured light
Close 28-32 DEG C of illumination anaerobism of bacterium and expand culture, cell concentration is detected after the completion of culture, cell concentration need to reach 105More than；
（4）Gamboge coccus is cultivated：Gamboge coccus strain is taken out and is inoculated in inoculum, the 30-32 DEG C of training in constant incubator
Support, then cultured 30-32 DEG C of gamboge coccus is expanded into culture, cell concentration need to reach 105More than；
Step 2, seed tank culture：Culture medium is added in the stainless steel seeding tank with agitating device, culture medium collocation method is such as
Under：The wt % of molasses 2, the wt % of peptone 0.5, yeast extract 0.5 wt %, K2HPO40.2wt%, MgSO4•7H2O 0.025
Wt %, remaining is water；It is inoculated with after sterilizing, wherein：Saccharomycete 2-4 wt %, lactic acid bacteria 4-6 wt %, photosynthetic bacteria 5-10
Wt %, gamboge coccus 2-4 wt %, remaining is water, and ferment 72-80h under the conditions of 30-32 DEG C, is stirred once every 1-2 hours,
Stirring 1-2h every time, i.e. stopping when pH value is reduced to 3-3.6, living bacteria count is up to 5 × 108CFU/mL；
Step 3：Culture medium is added in the fermentation tank with agitating device, culture medium collocation method is as follows：Molasses 2wt%, remaining
For water, room temperature, inoculation step are cooled to after sterilizing（2）In seed liquor, inoculum concentration 10wt%, fermented under the conditions of 30-32 DEG C
72-80h, stirred once every 1-2 hours, stir 1-2h every time, stopped when pH value is reduced to 3-3.6.
7. preparation method as claimed in claim 4, it is characterised in that step（Two）And step（Three）In for aeration culture.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109289500A (en) * | 2018-11-09 | 2019-02-01 | 山东卓华生物科技有限公司 | Microbial deodorant bacterium solution and preparation method thereof |
| CN110002695A (en) * | 2019-05-14 | 2019-07-12 | 山东省环科院环境工程有限公司 | A kind of leather-making waste water bio-synergistic processing method |
| CN112707587A (en) * | 2020-12-07 | 2021-04-27 | 天津天星科生皮革制品有限公司 | Wastewater treatment method in leather product production process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109289500A (en) * | 2018-11-09 | 2019-02-01 | 山东卓华生物科技有限公司 | Microbial deodorant bacterium solution and preparation method thereof |
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| CN112707587A (en) * | 2020-12-07 | 2021-04-27 | 天津天星科生皮革制品有限公司 | Wastewater treatment method in leather product production process |
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