CN110002695A - A kind of leather-making waste water bio-synergistic processing method - Google Patents

A kind of leather-making waste water bio-synergistic processing method Download PDF

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CN110002695A
CN110002695A CN201910396729.8A CN201910396729A CN110002695A CN 110002695 A CN110002695 A CN 110002695A CN 201910396729 A CN201910396729 A CN 201910396729A CN 110002695 A CN110002695 A CN 110002695A
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waste water
leather
parts
bacterium
sludge
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CN110002695B (en
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于军
邹晓凤
苏强
苟晓东
谢胜虎
谢少明
银德海
谭心
盖丽红
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ENVIRONMENTAL ENGINEERING Co Ltd SHANDONG ACADEMY OF ENVIRONMENTAL SCIENCE
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ENVIRONMENTAL ENGINEERING Co Ltd SHANDONG ACADEMY OF ENVIRONMENTAL SCIENCE
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F2001/007—Processes including a sedimentation step
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/22—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof
    • C02F2103/24—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof from tanneries
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/10—Solids, e.g. total solids [TS], total suspended solids [TSS] or volatile solids [VS]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/14—NH3-N
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used

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  • Life Sciences & Earth Sciences (AREA)
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  • Hydrology & Water Resources (AREA)
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  • Environmental & Geological Engineering (AREA)
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  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The present invention relates to a kind of leather-making waste water bio-synergistic processing methods, the strain fermentation step and waste water treatment step of fermented by mixed bacterium step, production lysozyme including efficient pollutant degradation bacteria.The present invention uses mode associated with " waste water induction+absorbent charcoal carrier coupling " in fermentation stage, by being oriented domestication to specific flora, strain being induced to adapt to wastewater environment in advance in fermenting step；In the fermenter using active carbon particle as core, thallus, which is adsorbed in, forms small zoogloea on active carbon, which can quickly be combined after entering in water treatment system with activated sludge, avoids strain loss；Meanwhile active carbon enter in system after can effectively remove leather-making waste water living system generation peculiar smell；In addition, passing through the study found that above-mentioned phenomenon is prominent in specific flora effect.

Description

A kind of leather-making waste water bio-synergistic processing method
Technical field
The present invention relates to a kind of leather-making waste water bio-synergistic processing methods, belong to Tannery Wastewater Treatment Process field.
Technical background
The water quality of leather-making waste water have complicated component, variation water quality are big, heavy metal chromium content is high, coloration is deep, suspended matter is high, The features such as easily biochemical.Wherein most representative feature is that suspended matter is high, heavy metal chromium content is high.Suspend owner in leather-making waste water It to be large quantities of lime, onal, hair, the dregs of fat and meat slag, suspended sediment concentration is up to thousands of milligrams per liter in waste water.Weight in waste water Chromic acid waste liquid discharged in crome metal main source and chrome tanning system.Since major pollutants are protein-based and oily in waste water Lipid material leads to Tannery Wastewater Treatment Technology generally existing sludge loading weight, oxygen demand is high, biochemical system stink is heavier etc. asks Topic.Requirement with China to sludge treatment and waste gas pollution control and treatment is increasingly strict, leather-making waste water biochemical treatment sludge smell control and Mud decrement problem is gradually taken seriously.
Bio-synergistic technology is by using additional nutrition, adds the dominant bacteria screened from nature or pass through gene The engineering strain of engineering technology building, add carrier or a series of technological means of improvement bioreactor etc. to improve biochemical systems micro- Bioactivity changes biochemical system Bacterial community, enhances biochemical system shock resistance to reach the treatment effeciency for improving biochemical system The purpose of ability.The technology is with strong points, without secondary pollution, easy to use.
Chinese patent literature CN105923928A (application number 201610521162.9) discloses a kind of the new of leather sewage Type processing method, this method uses the addition activation bacterium solution into conditioning tank and aeration tank to be aerated, to reduce sludge quantity.The party Method, which is deposited, to be lacked anoxic pond in process, is aerated the problems such as point is more, is caused total nitrogen to be difficult to remove, is consumed energy larger；Chinese patent text It offers CN101555086A (application number 200910084397.6) and discloses a kind of bioremediation of leather waste water, this method is adopted With two-stage AO tupe, biochemical process section is longer, and most of tannery wastewater treatment factory built up is difficult to realize；In State's patent document CN105948445A (application number 201610529256.0) discloses a kind of leather waste water sludge treatment technique, should Leather sludge is pumped into the reactor containing EM bacterium by method, and the temperature in reactor is risen to 40 after control humidity and dissolved oxygen ~45 DEG C, sludge press filtration is dehydrated after the reaction was continued 12~15h；This method needs to carry out heating treatment to reactor, for day The higher large-scale tannery wastewater treatment factory of sludge output is relatively difficult to achieve.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of leather-making waste water bio-synergistic processing methods.
The present invention adopts the following technical scheme:
A kind of leather-making waste water bio-synergistic processing method, fermented by mixed bacterium step including efficient pollutant degradation bacteria, Produce the strain fermentation step and waste water treatment step of lysozyme；
The fermented by mixed bacterium step of the efficient pollutant degradation bacteria, specific as follows:
(1) inoculation liquid is seeded in one grade fermemtation liquid in the ratio of percent by volume 5~10%, pH=6.5~ 8.0, it under conditions of 30~37 DEG C of temperature, 1.0~2.5VVM of ventilatory capacity, cultivates 28~48 hours, primary seed solution is made；
The one grade fermemtation liquid component is as follows, is parts by weight:
2~5 parts of Sugar cane molasses, 0.5~1 part of peptone, K2HPO4·3H20.02~0.05 part of O, MgSO40.01~ 0.03 part, FeSO40.01~0.03 part, 94~97 parts of water；
In the inoculation liquid containing effective bacterium be pseudomonad, bacillus licheniformis and bacillus pumilus, pseudomonad, The strain amount ratio of bacillus licheniformis and bacillus pumilus is 1:(1.5~2.5): (1.5~2.5)；
(2) primary seed solution made from step (1) is seeded to second level induction hair in the ratio of percent by volume 5~10% In zymotic fluid, under conditions of pH=6.5~8.0,30~37 DEG C of temperature, 1.0~2.5VVM of ventilatory capacity, cultivate 16~24 hours, Mixed bacteria liquid is made；
The second level induction fermentation liquid component is as follows, is parts by weight:
1~5 part of active powdered carbon, 2~5 parts of Sugar cane molasses, 0.5~1 part of peptone, K2HPO4·3H2O 0.02~0.05 Part, MgSO40.01~0.03 part, 0.01~0.03 part of FeSO4,25~40 parts of leather-making waste water, 40~70 parts of tap water；
The single culture fermentation step for producing lysozyme is that the strain concentration of culture preparation production lysozyme is 5.0 × 108 ~1.0 × 109The bacteriolyze bacterium solution of cfu/ml；
The waste water treatment step, specific as follows:
(i) leather-making waste water is adjusted into pH 7.5~8.5, then presses the anoxic sludge concentration item of 5000mg/L~8000mg/L Under part, waste water after anoxic treatment is made in 16~18h of anoxic treatment；
(ii) system is added by the continuous dosage of 5.0~15.0mg/L into waste water after anoxic treatment made from step (i) The mixed bacteria liquid obtained, then under the aerobic sludge concentration conditions of 8000mg/L~10000mg/L, 32~36h of Aerobic Process for Treatment, so Enter sedimentation basin afterwards through mud-water separation, processing water and aerobic sludge is made；
(iii) aerobic sludge made from step (ii), wherein 60~70% aerobic sludges are back to oxygen depletion system, residue 30 ~40% aerobic sludge enters sludge digestion pond, and bacteriolyze bacterium solution is added into sludge digestion pond, and the dosage of bacteriolyze bacterium solution is 15.0~30.0mg/L is handled through bacteriolyze, mud-water separation, and supernatant is mixed with the leather-making waste water in step (i).
Preferred according to the present invention, in the step (1), inoculation liquid cell concentration is 5.0 × 108~8.0 × 108cfu/ ml。
It is preferred according to the present invention, in the step (1), pseudomonad (Pseudomonas psychrotolerans) From China General Microbiological culture presevation administrative center, bacterium numbering 1.15631；Bacillus licheniformis (Bacillus Licheniformis China General Microbiological culture presevation administrative center, bacterium numbering 1.8791) are derived from；Short and small gemma bar Bacterium (Bacillus pumilus) derives from China General Microbiological culture presevation administrative center, bacterium numbering 1.7456.
It is preferred according to the present invention, in the step (1), pseudomonad, bacillus licheniformis and bacillus pumilus Strain amount ratio is 1:(1.5~2.5): (1.5~2.5).
Preferred according to the present invention, in the step (2), active carbon powder diameter is 200 mesh.
Preferred according to the present invention, the single culture fermentation step for producing lysozyme is specific as follows:
(a) the single culture bacterium solution for producing lysozyme is seeded to level-one culture solution in the ratio of percent by volume 5~10% In, under conditions of 30~37 DEG C, bacteriolyze seed liquor is made in ventilation culture 28~48 hours；
The component of the level-one culture solution is as follows, is parts by weight:
0.5~0.8 part of corn flour, 0.2~0.5 part of Sugar cane molasses, 0.3~1.0 part of peptone, 0.3~0.5 part of NaCl, K2HPO4·3H20.02~0.05 part of O, MgSO40.01~0.03 part, CaCl20.002~0.005 part, MnSO40.001~ 0.005 part, 94~98 parts of water；
(b) bacteriolyze seed liquor made from step (a) is seeded to second level culture solution in the ratio of percent by volume 5~10% In, under conditions of 30~37 DEG C, bacteriolyze bacterium solution is made in ventilation culture 16~24 hours；
25~40 parts of leather-making waste water, active 1~5 part of powdered carbon, 0.5~0.8 part of corn flour, 0.2~0.5 part of Sugar cane molasses, 0.3~1.0 part of peptone, NaCl0.3~0.5 part, K2HPO4·3H20.02~0.05 part of O, MgSO40.01~0.03 part, CaCl20.002~0.005 part, MnSO40.001~0.005 part, 40~75 parts of water.
According to the present invention it is further preferred that in the step (b), the partial size of active powdered carbon is 200 mesh.
Preferred according to the present invention, the single culture of the production lysozyme is the burnt auspicious molten bacillus (Lysobacter of Shen Shi Ruishenii), China General Microbiological culture presevation administrative center, bacterium numbering 1.10136 are derived from.
Preferred according to the present invention, the leather-making waste water is to obtain after grid removes impurity.
Preferred according to the present invention, a part in aerobic sludge precipitated in the step (ii) flows back into anoxic System；It is further preferred that aerobic sludge reuse amount is the 60~70% of aerobic sludge total amount.
Preferred according to the present invention, dissolved oxygen is 2.0~4.0mg/L in the step (ii).
Preferred according to the present invention, the aerobic sludge that bacteriolyze bacterium solution is added in the step (iii) accounts for aerobic sludge total amount 30~40%.
Beneficial effect
1, the present invention uses mode associated with " waste water induction+absorbent charcoal carrier coupling " in fermentation stage, in fermenting step In by specific flora be oriented domestication, induction strain adapt to wastewater environment in advance；In the fermenter with active carbon particle For core, thallus, which is adsorbed in, forms small zoogloea on active carbon, which can be with activity after entering in water treatment system Sludge quickly combines, and avoids strain loss；Meanwhile active carbon enter in system after can effectively remove leather-making waste water living system The peculiar smell of generation；In addition, passing through the study found that above-mentioned phenomenon is prominent in specific flora effect；
2, the present invention clears up sludge using molten bacillus, which has the function of producing lysozyme, and energy useful effect is in leather Lan Shi positive bacteria, acinetobacter calcoaceticus, the common flora of proteus isoreactivity sludge, generating bacteriolysis makes cellular lysate to reach The purpose of mud decrement.Relative to other mud decrement modes, this method investment is low, effect is good, without secondary pollution, is handling this When applying for the sludge generated, it can be reduced 60% or more, and there is specificity by experiment；
3, the present invention passes through company by " live fermentation+bio-synergistic " organic assembling for the water quality characteristics of leather-making waste water Continuing and adds microbial inoculum into aerobic tank and sludge digestion pond, the strain added forms stable dominant group in activated sludge, COD removal rate is added up to 90% or more, compared to long term purchase strain to biochemical system, and this method saves 80% or more cost. Realize that treatment effect is good, processing cost is cheap, the convenient technological effect of operational management, reached COD in waste water, ammonia nitrogen and The efficient removal of the pollutants index such as stink, and mud decrement can be effectively carried out, there is distinguishing feature economic, efficiently, environmentally friendly.
Specific embodiment
Technical solution of the present invention is further elaborated below with reference to embodiment, but institute's protection scope of the present invention is not limited to This.
Biological material source
Pseudomonad (Pseudomonas psychrotolerans) derives from China General Microbiological culture presevation management Center, bacterium numbering 1.15631 are existing non-preservation strain；
Bacillus licheniformis (Bacillus licheniformis) derives from China General Microbiological culture presevation management Center, bacterium numbering 1.8791 are existing non-preservation strain；
Bacillus pumilus (Bacillus pumilus) derives from China General Microbiological culture presevation administrative center, bacterium Kind number 1.7456 is existing non-preservation strain；
The burnt auspicious molten bacillus of Shen Shi (Lysobacter ruishenii) derives from China General Microbiological culture presevation management Center, bacterium numbering 1.10136 are existing non-preservation strain；
Detection method
CODCrDetection uses HJ-828-2017, and ammonia nitrogen detection uses HJ 535-2009, and MLSS detection uses gravimetric method.
Embodiment 1
A kind of leather-making waste water bio-synergistic processing method, fermented by mixed bacterium step including efficient pollutant degradation bacteria, Produce the strain fermentation step and waste water treatment step of lysozyme；
The fermented by mixed bacterium step of the efficient pollutant degradation bacteria, specific as follows:
It (1) is 6.0 × 10 by cell concentration8The inoculation liquid of cfu/ml is seeded to level-one in the ratio of percent by volume 10% In fermentation liquid, under conditions of pH=7.0,30 DEG C of temperature, ventilatory capacity 2.0VVM, cultivates 30 hours, primary seed solution is made；
The one grade fermemtation liquid component is as follows, is parts by weight:
2 parts of Sugar cane molasses, 0.5 part of peptone, K2HPO4·3H20.02 part of O, MgSO40.01 part, FeSO40.01 part, 97 parts of water；
In the inoculation liquid containing effective bacterium be pseudomonad, bacillus licheniformis and bacillus pumilus, pseudomonad, The strain amount ratio of bacillus licheniformis and bacillus pumilus is 1:1.5:1.5；
(2) primary seed solution made from step (1) is seeded to second level induction fermentation in the ratio of percent by volume 10% In liquid, under conditions of pH=7.0,37 DEG C of temperature, ventilatory capacity 2.0VVM, cultivates 16 hours, mixed bacteria liquid is made；
The second level induction fermentation liquid component is as follows, is parts by weight:
Partial size is 0.5 part of 2 parts of 1 part of active powdered carbon, Sugar cane molasses, peptone, the K of 200 mesh2HPO4·3H20.02 part of O, MgSO40.01 part, 0.01 part of FeSO4,25 parts of leather-making waste water, 70 parts of tap water；
The strain fermentation step for producing lysozyme is that the strain concentration of culture preparation production lysozyme is 6.2 × 108cfu/ The bacteriolyze bacterium solution of ml；It is specific as follows:
(a) the single culture bacterium solution for producing lysozyme is seeded in level-one culture solution in the ratio of percent by volume 10%, Under conditions of 30 DEG C, seed liquor is made in ventilation culture 28 hours；
The component of the level-one culture solution is as follows, is parts by weight:
0.5 part of corn flour, 0.2 part of Sugar cane molasses, 0.3 part of peptone, 0.3 part of NaCl, K2HPO4·3H20.02 part of O, MgSO40.01 part, CaCl20.002 part, MnSO40.001 part, 98 parts of water；
(b) bacteriolyze seed liquor made from step (a) is seeded in second level culture solution in the ratio of percent by volume 10%, Under conditions of 30 DEG C, bacteriolyze bacterium solution is made in ventilation culture 16 hours；
25 parts of leather-making waste water, 0.2 part of 0.5 part of 1 part of active powdered carbon, corn flour, Sugar cane molasses, the albumen that partial size is 200 mesh 0.3 part of peptone, NaCl0.3 parts, K2HPO4·3H20.02 part of O, MgSO40.01 part, CaCl20.002 part, MnSO40.001 part, 73 parts of water.
The waste water treatment step, specific as follows:
(i) then the leather-making waste water obtained after grid removes impurity is adjusted into pH 7.5 by 6000mg/L anoxic sludge Under concentration conditions, waste water after anoxic treatment is made in anoxic treatment 16h；
(ii) it is added into waste water after anoxic treatment made from step (i) by the continuous dosage of 5.0mg/L obtained mixed Combined bacteria liquid, then under conditions of the aerobic sludge concentration of 8000mg/L, dissolved oxygen are 2.0mg/L, Aerobic Process for Treatment 32h, then Through mud-water separation, processing water and aerobic sludge is made；
A part in the step (ii) in aerobic sludge obtained and the leather-making waste water mixing reuse in step (i)； Aerobic sludge regurgitant volume is the 60% of aerobic sludge total amount；
(iii) aerobic sludge made from step (ii), wherein 60% aerobic sludge is back to oxygen depletion system, residue 40% is good Oxygen sludge enters sludge digestion pond, and bacteriolyze bacterium solution is added into sludge digestion pond, and the dosage of bacteriolyze bacterium solution is 30.0mg/L, warp Bacteriolyze processing, mud-water separation, supernatant are mixed with the leather-making waste water in step (i).
Embodiment 2
A kind of leather-making waste water bio-synergistic processing method, fermented by mixed bacterium step including efficient pollutant degradation bacteria, Produce the strain fermentation step and waste water treatment step of lysozyme；
The fermented by mixed bacterium step of the efficient pollutant degradation bacteria, specific as follows:
It (1) is 6.0 × 10 by cell concentration8The inoculation liquid of cfu/ml is seeded to level-one in the ratio of percent by volume 10% In fermentation liquid, under conditions of pH=8.0,37 DEG C of temperature, ventilatory capacity 2.5VVM, cultivates 48 hours, primary seed solution is made；
The one grade fermemtation liquid component is as follows, is parts by weight:
5 parts of Sugar cane molasses, 1 part of peptone, K2HPO4·3H20.05 part of O, MgSO40.03 part, FeSO40.03 part, water 94 parts；
In the inoculation liquid containing effective bacterium be pseudomonad, bacillus licheniformis and bacillus pumilus, pseudomonad, The strain amount ratio of bacillus licheniformis and bacillus pumilus is 1:2.5:2.5；
(2) primary seed solution made from step (1) is seeded to second level induction fermentation liquid in the ratio of percent by volume 5% In, it under conditions of pH=8.0,37 DEG C of temperature, ventilatory capacity 2.5VVM, cultivates 24 hours, mixed bacteria liquid is made；
The second level induction fermentation liquid component is as follows, is parts by weight:
Partial size is 1 part of 5 parts of 5 parts of active powdered carbon, Sugar cane molasses, peptone, the K of 200 mesh2HPO4·3H20.05 part of O, MgSO40.03 part, 0.03 part of FeSO4,40 parts of leather-making waste water, 49 parts of tap water；
The single culture fermentation step for producing lysozyme be culture preparation produce the strain concentration of lysozyme be 7.2 × 108The bacteriolyze bacterium solution of cfu/ml；It is specific as follows:
(a) the single culture bacterium solution for producing lysozyme is seeded in level-one culture solution in the ratio of percent by volume 5%, Under conditions of 37 DEG C, seed liquor is made in ventilation culture 48 hours；
The component of the level-one culture solution is as follows, is parts by weight:
0.8 part of corn flour, 0.5 part of Sugar cane molasses, 1.0 parts of peptone, 0.5 part of NaCl, K2HPO4·3H20.05 part of O, MgSO40.03 part, CaCl20.005 part, MnSO40.005 part, 97 parts of water；
(b) bacteriolyze seed liquor made from step (a) is seeded in second level culture solution in the ratio of percent by volume 5%, Under conditions of 37 DEG C, bacteriolyze bacterium solution is made in ventilation culture 24 hours；
40 parts of leather-making waste water, 0.5 part of 0.8 part of 5 parts of active powdered carbon, corn flour, Sugar cane molasses, the albumen that partial size is 200 mesh 1.0 parts of peptone, NaCl0.5 parts, K2HPO4·3H20.05 part of O, MgSO40.03 part, CaCl20.005 part, MnSO40.005 part, 52 parts of water.
The waste water treatment step, specific as follows:
(i) then the leather-making waste water obtained after grid removes impurity is adjusted into pH 7.5 by 6000mg/L anoxic sludge Under concentration conditions, waste water after anoxic treatment is made in anoxic treatment 16h；
(ii) it is added into waste water after anoxic treatment made from step (i) by the continuous dosage of 15.0mg/L obtained mixed Combined bacteria liquid, then under conditions of the aerobic sludge concentration of 8000mg/L, dissolved oxygen are 2.0mg/L, Aerobic Process for Treatment 32h, then Through mud-water separation, processing water and aerobic sludge is made；
A part in the step (ii) in aerobic sludge obtained and the leather-making waste water mixing reuse in step (i)； Aerobic sludge regurgitant volume is the 60% of aerobic sludge total amount；
(iii) aerobic sludge made from step (ii), wherein 60% aerobic sludge is back to oxygen depletion system, residue 40% is good Oxygen sludge enters sludge digestion pond, and bacteriolyze bacterium solution is added into sludge digestion pond, and the dosage of bacteriolyze bacterium solution is 30.0mg/L, warp Bacteriolyze processing, mud-water separation, supernatant are mixed with the leather-making waste water in step (i).
Comparative example 1
Leather-making waste water bio-synergistic processing method as described in Example 1, the difference is that, pseudomonad is replaced with Aeromonas (Aeromonas Salmonicida)
Containing effective bacterium in inoculation liquid is Aeromonas, bacillus licheniformis and bacillus pumilus, Aeromonas, lichens The strain amount ratio of bacillus and bacillus pumilus is 1:1.5:1.5；
The Aeromonas (Aeromonas Salmonicida) derives from China General Microbiological culture presevation management Center, bacterium numbering 1.16014；
Comparative example 2
Leather-making waste water bio-synergistic processing method as described in Example 1, the difference is that, modification produces the coke of lysozyme The auspicious molten bacillus of Shen Shi (Lysobacter ruishenii) is the molten bacillus in Chengdu (Lysobacter chengduensis)
The molten bacillus in the Chengdu (Lysobacter chengduensis) derives from China General Microbiological culture presevation Administrative center, bacterium numbering 1.15145；
Comparative example 3
Leather-making waste water bio-synergistic processing method as described in Example 1, the difference is that, by step (2) and step (b) leather-making waste water in second order fermentation liquid component replaces with tap water.
Comparative example 4
Leather-making waste water bio-synergistic processing method as described in Example 1, the difference is that, by step (2) and step (b) the active powdered carbon in replaces with diatomite.
Experimental example 1
Select the tannery wastewater treatment factory of Hebei Xinji leather manufacturing enterprise as experimental subjects, index of intaking are as follows: CODCr1150~1280mg/L, 124~149mg/L of ammonia nitrogen, pH7.4~7.6 are discharged COD before synergism experimentCr220~ 280mg/L, ammonia nitrogen 12~20mg/L days sludge volume (moisture content 80%) 15t/d.
Embodiment 1, embodiment 2, comparative example 1, comparative example 2, comparative example 3, method described in comparative example 4 is respectively adopted to system It removes from office waste water and carries out synergy processing, every kind of method processing time is 30 days.Daily anoxic pond, aerobic tank sludge concentration are recorded, is controlled Anoxic pond sludge concentration processed is in 6000mg/L or so, and aerobic tank sludge concentration is in 8000mg/L or so.Daily measurement water outlet CODCr Value, ammonia nitrogen value, record daily sludge volume.It is used as the system synergy phase within 15 days before synergy, is used as system synergy stationary phase within 16~30 days, It detects and is discharged COD in stationary phaseCrValue, ammonia nitrogen value and sludge volume, are averaged, the results are shown in Table 1:
Table 1
Interpretation of result
Efficient pollutant degradation bacteria is only that according to the difference that the data of table 1 can be seen that embodiment 1 and comparative example 1 One of mixed bacteria difference, so as to cause the notable difference of effluent quality, this is to illustrate the combination pair of three kinds of bacterium of the application The processing of such waste water has significant technical effect, if similar bacterium is replaced with by any, under effect has significantly Drop；In addition, equally will affect by the mixed bacteria collocation that day spoil disposal figureofmerit can be seen that efficient pollutant degradation bacteria subsequent Treatment of Sludge effect is easier to be cleared up by lysozyme using the sludge that the mixed bacteria collocation of the application generates；
It is only that the bacterial strain for producing lysozyme is different according to the difference that the data of table 1 can be seen that embodiment 1 and comparative example 2, So as to cause the notable difference of day sludge volume, this illustrates that the sludge that the mixed bacteria collocation of the application generates is easier by specified germ The lysozyme resolution that strain generates has relatively more significant specificity；
It is only that the bacterial strain for producing lysozyme does not have according to the difference that the data of table 1 can be seen that embodiment 1 and comparative example 3 Step is tamed, the water quality data so as to cause day sludge volume and processing water has notable difference, this illustrates to tame step to waste water The technical effect of the bacterial strain of processing bacterium and production lysozyme all has significant impact；
It is only that adsorption component is not identical according to the difference that the data of table 1 can be seen that embodiment 1 and comparative example 4, thus Cause day sludge volume and the water quality data for handling water that there is notable difference, is especially discharged the index of ammonia nitrogen, difference is obvious, this says Bright adsorption component will be significantly higher than COD to the ammonia nitrogen influence in mixed bacteria removal waste waterCrIndex, it was demonstrated that adsorption component active carbon Influence to technical effect has specificity.

Claims (10)

1. a kind of leather-making waste water bio-synergistic processing method, which is characterized in that the mixed bacteria including efficient pollutant degradation bacteria Fermentation step, the strain fermentation step and waste water treatment step for producing lysozyme；
The fermented by mixed bacterium step of the efficient pollutant degradation bacteria, specific as follows:
(1) inoculation liquid is seeded in one grade fermemtation liquid in the ratio of percent by volume 5~10%, in pH=6.5~8.0, temperature It under conditions of 30~37 DEG C of degree, 1.0~2.5VVM of ventilatory capacity, cultivates 28~48 hours, primary seed solution is made；
The one grade fermemtation liquid component is as follows, is parts by weight:
2~5 parts of Sugar cane molasses, 0.5~1 part of peptone, K2HPO4·3H20.02~0.05 part of O, MgSO40.01~0.03 part, FeSO40.01~0.03 part, 94~97 parts of water；
Containing effective bacterium in the inoculation liquid is pseudomonad, bacillus licheniformis and bacillus pumilus, pseudomonad, lichens The strain amount ratio of bacillus and bacillus pumilus is 1:(1.5~2.5): (1.5~2.5)；
(2) primary seed solution made from step (1) is seeded to second level induction fermentation liquid in the ratio of percent by volume 5~10% In, it under conditions of pH=6.5~8.0,30~37 DEG C of temperature, 1.0~2.5VVM of ventilatory capacity, cultivates 16~24 hours, is made Mixed bacteria liquid；
The second level induction fermentation liquid component is as follows, is parts by weight:
1~5 part of active powdered carbon, 2~5 parts of Sugar cane molasses, 0.5~1 part of peptone, K2HPO4·3H20.02~0.05 part of O, MgSO40.01~0.03 part, 0.01~0.03 part of FeSO4,25~40 parts of leather-making waste water, 40~70 parts of tap water；
The single culture fermentation step for producing lysozyme is that the strain concentration of culture preparation production lysozyme is 5.0 × 108~1.0 ×109The bacteriolyze bacterium solution of cfu/ml；
The waste water treatment step, specific as follows:
(i) leather-making waste water is adjusted into pH 7.5~8.5, then presses the anoxic sludge concentration conditions of 5000mg/L~8000mg/L Under, waste water after anoxic treatment is made in 16~18h of anoxic treatment；
(ii) it is added into waste water after anoxic treatment made from step (i) by the continuous dosage of 5.0~15.0mg/L obtained Mixed bacteria liquid, then under the aerobic sludge concentration conditions of 8000mg/L~10000mg/L, 32~36h of Aerobic Process for Treatment, then into Enter sedimentation basin through mud-water separation, processing water and aerobic sludge is made；
(iii) aerobic sludge made from step (ii), wherein 60~70% aerobic sludges are back to oxygen depletion system, residue 30~ 40% aerobic sludge enters sludge digestion pond, and bacteriolyze bacterium solution is added into sludge digestion pond, and the dosage of bacteriolyze bacterium solution is 15.0 ~30.0mg/L, is handled through bacteriolyze, mud-water separation, and supernatant is mixed with the leather-making waste water in step (i).
2. processing method as described in claim 1, which is characterized in that in the step (1), inoculation liquid cell concentration is 5.0 ×108~8.0 × 108cfu/ml。
3. processing method as described in claim 1, which is characterized in that in the step (1), pseudomonad (Pseudomonas Psychrotolerans China General Microbiological culture presevation administrative center, bacterium numbering 1.15631) are derived from；Lichens gemma Bacillus (Bacillus licheniformis) derives from China General Microbiological culture presevation administrative center, bacterium numbering 1.8791；Bacillus pumilus (Bacillus pumilus) derives from China General Microbiological culture presevation administrative center, bacterium Kind number 1.7456.
4. processing method as described in claim 1, which is characterized in that in the step (1), pseudomonad, lichens gemma bar The strain amount ratio of bacterium and bacillus pumilus is 1:(1.5~2.5): (1.5~2.5).
5. processing method as described in claim 1, which is characterized in that in the step (2), active carbon powder diameter is 200 mesh.
6. processing method as described in claim 1, which is characterized in that the single culture fermentation step for producing lysozyme, tool Body is as follows:
(a) the single culture bacterium solution for producing lysozyme is seeded in level-one culture solution in the ratio of percent by volume 5~10%, Under conditions of 30~37 DEG C, bacteriolyze seed liquor is made in ventilation culture 28~48 hours；
The component of the level-one culture solution is as follows, is parts by weight:
0.5~0.8 part of corn flour, 0.2~0.5 part of Sugar cane molasses, 0.3~1.0 part of peptone, 0.3~0.5 part of NaCl, K2HPO4·3H20.02~0.05 part of O, MgSO40.01~0.03 part, CaCl20.002~0.005 part, MnSO40.001~ 0.005 part, 94~98 parts of water；
(b) bacteriolyze seed liquor made from step (a) is seeded in second level culture solution in the ratio of percent by volume 5~10%, Under conditions of 30~37 DEG C, bacteriolyze bacterium solution is made in ventilation culture 16~24 hours；
25~40 parts of leather-making waste water, active 1~5 part of powdered carbon, 0.5~0.8 part of corn flour, 0.2~0.5 part of Sugar cane molasses, albumen 0.3~1.0 part of peptone, NaCl0.3~0.5 part, K2HPO4·3H20.02~0.05 part of O, MgSO40.01~0.03 part, CaCl20.002~0.005 part, MnSO40.001~0.005 part, 40~75 parts of water.
7. processing method as claimed in claim 6, which is characterized in that in the step (b), the partial size of active powdered carbon is 200 Mesh；
Preferably, the single culture of the production lysozyme is the burnt auspicious molten bacillus of Shen Shi (Lysobacter ruishenii), is come Derived from China General Microbiological culture presevation administrative center, bacterium numbering 1.10136.
8. processing method as described in claim 1, which is characterized in that the leather-making waste water is to obtain after grid removes impurity ?.
9. processing method as described in claim 1, which is characterized in that in the aerobic sludge precipitated in the step (ii) A part flows back into oxygen depletion system；It is further preferred that aerobic sludge reuse amount is the 60~70% of aerobic sludge total amount；
Preferably, dissolved oxygen is 2.0~4.0mg/L in the step (ii).
10. processing method as described in claim 1, which is characterized in that the aerobic of bacteriolyze bacterium solution is added in the step (iii) Sludge accounts for the 30~40% of aerobic sludge total amount.
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