CN110002695B - Biological synergistic treatment method for tanning wastewater - Google Patents

Biological synergistic treatment method for tanning wastewater Download PDF

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CN110002695B
CN110002695B CN201910396729.8A CN201910396729A CN110002695B CN 110002695 B CN110002695 B CN 110002695B CN 201910396729 A CN201910396729 A CN 201910396729A CN 110002695 B CN110002695 B CN 110002695B
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wastewater
parts
sludge
strain
fermentation
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CN110002695A (en
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于军
邹晓凤
苏强
苟晓东
谢胜虎
谢少明
银德海
谭心
盖丽红
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Environmental Engineering Co ltd Shandong Academy Of Environmental Science
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F2001/007—Processes including a sedimentation step
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/22—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof
    • C02F2103/24—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof from tanneries
    • C—CHEMISTRY; METALLURGY
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    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/10—Solids, e.g. total solids [TS], total suspended solids [TSS] or volatile solids [VS]
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    • C02F2209/14—NH3-N
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    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used

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Abstract

The invention relates to a biological synergistic treatment method of tannery wastewater, which comprises a mixed strain fermentation step of high-efficiency pollutant degrading bacteria, a strain fermentation step of lysozyme production and a wastewater treatment step. In the fermentation stage, a mode of combining wastewater induction and activated carbon carrier coupling is adopted, and in the fermentation link, specific flora is subjected to directional domestication to induce strains to adapt to the wastewater environment in advance; activated carbon particles are taken as a core in a fermentation tank, thalli are adsorbed on the activated carbon to form tiny zoogles, and the zoogles can be quickly combined with activated sludge after entering a water treatment system, so that strain loss is avoided; meanwhile, after the activated carbon enters the system, the peculiar smell generated by the tanning wastewater living system can be effectively removed; in addition, it has been found through studies that the above phenomenon is remarkably effective in a specific flora.

Description

Biological synergistic treatment method for tanning wastewater
Technical Field
The invention relates to a biological synergistic treatment method for tannery wastewater, belonging to the technical field of tannery wastewater treatment.
Technical Field
The water quality of the tannery wastewater has the characteristics of complex components, large water quality fluctuation, high heavy metal chromium content, deep chromaticity, high suspended matter content, easy biochemistry and the like. The most representative characteristics are high suspended matter content and high heavy metal chromium content. The suspended matters in the tannery wastewater mainly comprise a large amount of lime, crushed leather, wool, oil residue and meat residue, and the concentration of suspended solids in the wastewater reaches thousands of milligrams per liter. The main source of heavy metal chromium in the wastewater and chromic acid wastewater liquid discharged in the chrome tanning. As the main pollutants in the wastewater are protein and grease substances, the leather-making wastewater treatment process generally has the problems of heavy load of sludge, high oxygen consumption, heavy odor of a biochemical system and the like. Along with the increasingly strict requirements of China on sludge treatment and waste gas treatment, the problems of sludge odor control and sludge reduction in the biochemical treatment of tannery wastewater are gradually emphasized.
The biological synergistic technology is characterized in that the microbial activity of a biochemical system is improved by adopting a series of technical means of adding nutrition, adding dominant strains screened from nature or engineering strains constructed by a genetic engineering technology, adding a carrier or improving a bioreactor and the like, so that the aims of improving the treatment efficiency of the biochemical system, changing the flora structure of the biochemical system and enhancing the shock resistance of the biochemical system are fulfilled. The technology has strong pertinence, no secondary pollution and convenient use.
Chinese patent document CN105923928A (application No. 201610521162.9) discloses a novel treatment method for leather sewage, which adds activated bacteria liquid into a regulating tank and an aeration tank for aeration to reduce the amount of sludge. The method has the problems of lack of an anoxic tank, more aeration points and the like in the process, so that the total nitrogen is difficult to remove, and the energy consumption is higher; chinese patent document CN101555086A (application No. 200910084397.6) discloses a biological treatment method of leather wastewater, which adopts a two-stage AO treatment mode, has a long biochemical process section and is difficult to realize by most of the established leather wastewater treatment plants; chinese patent document CN105948445A (application No. 201610529256.0) discloses a leather wastewater sludge treatment process, which comprises the steps of pumping leather sludge into a reactor containing EM bacteria, controlling humidity and dissolved oxygen, raising the temperature in the reactor to 40-45 ℃, continuously reacting for 12-15 hours, and then carrying out filter pressing and dehydration on the sludge; the method needs to heat the reactor, and is difficult to realize in large-scale tanning wastewater treatment plants with high daily sludge yield.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a biological synergistic treatment method for tannery wastewater.
The invention adopts the following technical scheme:
a tanning wastewater biological synergistic treatment method comprises a mixed strain fermentation step of high-efficiency pollutant degrading bacteria, a lysozyme-producing strain fermentation step and a wastewater treatment step;
the fermentation step of the mixed strain of the high-efficiency pollutant degrading bacteria comprises the following steps:
(1) inoculating the inoculation liquid into the primary fermentation liquid according to the volume percentage of 5-10%, and culturing for 28-48 hours under the conditions that the pH is 6.5-8.0, the temperature is 30-37 ℃ and the ventilation volume is 1.0-2.5 VVM to prepare primary seed liquid;
the primary fermentation liquid comprises the following components in parts by weight:
2-5 parts of cane sugar molasses, 0.5-1 part of peptone and K2HPO4·3H20.02-0.05 part of O and MgSO40.01 to 0.03 part of FeSO40.01-0.03 part of water and 94-97 parts of water;
the inoculation liquid contains effective bacteria such as pseudomonas, bacillus licheniformis and bacillus pumilus, and the ratio of the strains of the pseudomonas, the bacillus licheniformis and the bacillus pumilus is 1: (1.5-2.5): (1.5-2.5);
(2) inoculating the primary seed solution prepared in the step (1) into secondary induction fermentation liquor according to the volume percentage of 5-10%, and culturing for 16-24 hours under the conditions that the pH is 6.5-8.0, the temperature is 30-37 ℃, and the ventilation volume is 1.0-2.5 VVM, so as to prepare a mixed bacterial solution;
the secondary induction fermentation broth comprises the following components in parts by weight:
1-5 parts of activated carbon powder, 2-5 parts of cane sugar molasses, 0.5-1 part of peptone and K2HPO4·3H20.02-0.05 part of O and MgSO40.01-0.03 part of FeSO40.01-0.03 part of tannery wastewater, 25-40 parts of tannery wastewater and 40-70 parts of tap water;
the fermentation step of the single strain for producing the lysozyme is to culture and prepare the strain for producing the lysozyme with the concentration of 5.0 multiplied by 108～1.0×109cfu/ml of bacteriolytic liquid;
the wastewater treatment steps are as follows:
(i) adjusting the pH value of the tannery wastewater to 7.5-8.5, and then carrying out anoxic treatment for 16-18 h under the condition of anoxic sludge concentration of 5000-8000 mg/L to prepare anoxic-treated wastewater;
(ii) adding the prepared mixed bacterial liquid into the anoxic-treated wastewater prepared in the step (i) according to the continuous adding amount of 5.0-15.0 mg/L, carrying out aerobic treatment for 32-36 h under the condition of the aerobic sludge concentration of 8000 mg/L-10000 mg/L, and then carrying out sludge-water separation in a sedimentation tank to prepare treated water and aerobic sludge;
(iii) and (3) refluxing 60-70% of the aerobic sludge prepared in the step (ii) to an anoxic system, allowing the rest 30-40% of the aerobic sludge to enter a sludge digestion tank, adding a bacteriolytic solution into the sludge digestion tank, wherein the addition amount of the bacteriolytic solution is 15.0-30.0 mg/L, performing bacteriolytic treatment, performing sludge-water separation, and mixing the supernatant with the tannery wastewater in the step (i).
Preferably, in the step (1), the cell concentration of the inoculum is 5.0X 108～8.0×108cfu/ml。
Preferably, in the step (1), the Pseudomonas (Pseudomonas pseudorhizogenes) is originated from the China general microbiological culture Collection center, with the strain number of 1.15631; bacillus licheniformis (Bacillus licheniformis) is from China general microbiological culture Collection center with strain number 1.8791; the Bacillus pumilus (Bacillus pumilus) is derived from China general microbiological culture Collection center with the strain number of 1.7456.
Preferably, in the step (1), the ratio of the strains of pseudomonas, bacillus licheniformis and bacillus pumilus is 1: (1.5-2.5): (1.5-2.5).
Preferably, in step (2), the particle size of the activated carbon powder is 200 meshes.
According to the invention, the fermentation step of the single strain for producing the lysozyme is as follows:
(a) inoculating the lysozyme-producing single strain bacterial liquid into the primary culture solution according to the volume percentage of 5-10%, and performing aerobic culture for 28-48 hours at the temperature of 30-37 ℃ to obtain a bacteriolysis seed liquid;
the first-stage culture solution comprises the following components in parts by weight:
0.5-0.8 part of corn flour, 0.2-0.5 part of cane sugar molasses, 0.3-1.0 part of peptone, 0.3-0.5 part of NaCl, and K2HPO4·3H20.02-0.05 part of O and MgSO40.01 to 0.03 portion of CaCl20.002 to 0.005 part by weight, MnSO40.001-0.005 part of water and 94-98 parts of water;
(b) inoculating the bacteriolytic seed solution prepared in the step (a) into a secondary culture solution according to the volume percentage of 5-10%, and performing aerated culture for 16-24 hours at the temperature of 30-37 ℃ to prepare bacteriolytic liquid;
25-40 parts of tannery wastewater, 1-5 parts of activated carbon powder, 0.5-0.8 part of corn flour, 0.2-0.5 part of cane sugar molasses, 0.3-1.0 part of peptone, 0.3-0.5 part of NaCl0.3-0.5 part of K2HPO4·3H20.02-0.05 part of O and MgSO40.01 to 0.03 portion of CaCl20.002 to 0.005 part by weight, MnSO40.001 to 0.005 part of water and 40 to 75 parts of water.
According to a further preferred embodiment of the present invention, in the step (b), the particle size of the activated carbon powder is 200 mesh.
According to the invention, the single strain for producing the lysozyme is Lysobacter xylinum (Lysobacter ruischeni) which is derived from China general microbiological culture collection center and has the strain number of 1.10136.
According to the invention, the tannery wastewater is preferably obtained after impurities are removed through a grating.
Preferably according to the present invention, a part of the aerobic sludge precipitated in the step (ii) is returned to the anoxic system; further preferably, the recycling amount of the aerobic sludge is 60-70% of the total amount of the aerobic sludge.
According to the invention, the dissolved oxygen in the step (ii) is preferably 2.0-4.0 mg/L.
According to the invention, the aerobic sludge added with the lysozyme liquid in the step (iii) accounts for 30-40% of the total amount of the aerobic sludge.
Advantageous effects
1. In the fermentation stage, a mode of combining wastewater induction and activated carbon carrier coupling is adopted, and in the fermentation link, specific flora is subjected to directional domestication to induce strains to adapt to the wastewater environment in advance; activated carbon particles are taken as a core in a fermentation tank, thalli are adsorbed on the activated carbon to form tiny zoogles, and the zoogles can be quickly combined with activated sludge after entering a water treatment system, so that strain loss is avoided; meanwhile, after the activated carbon enters the system, the peculiar smell generated by the tanning wastewater living system can be effectively removed; in addition, the research shows that the phenomenon has prominent effect on specific flora;
2. the method adopts lysobacter to digest the sludge, the lysobacter has the function of producing lysozyme, can effectively act on common floras of the activated sludge such as gram-positive bacteria, acinetobacter, proteus and the like, and produces the bacteriolysis to crack the thalli so as to achieve the purpose of sludge reduction. Compared with other sludge reduction modes, the method has the advantages of low investment, good effect and no secondary pollution, can reduce the sludge generated by the method by more than 60 percent when the sludge is treated, and has specificity through experiments;
3. aiming at the water quality characteristics of the tannery wastewater, the method has the advantages that by means of the organic combination of 'field fermentation and biological synergism', and by continuously adding the microbial inoculum into the aerobic tank and the sludge digestion tank, the added strains form stable dominant groups in the activated sludge, the COD removal rate can reach over 90 percent, and compared with the method of adding the strains into a biochemical system after long-term purchase, the method saves the cost by over 80 percent. The method has the advantages of realizing the process effects of good treatment effect, low treatment cost and convenient operation and management, achieving the high-efficiency removal of pollutants such as COD, ammonia nitrogen, odor and the like in the wastewater, effectively reducing the sludge, and having the obvious characteristics of economy, high efficiency and environmental protection.
Detailed Description
The technical solution of the present invention is further described with reference to the following examples, but the scope of the present invention is not limited thereto.
Source of biological material
Pseudomonas (Pseudomonas pseudomonads) comes from China general microbiological culture collection management center, and has a culture number of 1.15631, and is an existing non-collection strain;
bacillus licheniformis (Bacillus licheniformis) is from China general microbiological culture Collection center, has a culture number of 1.8791, and is an existing non-preserved strain;
the Bacillus pumilus (Bacillus pumilus) is derived from China general microbiological culture collection center, has a culture number of 1.7456 and is an existing non-preserved strain;
lysobacter darriensis (Lysobacter ruischeni) is derived from the China general microbiological culture collection center, has the culture number of 1.10136 and is an existing non-collection strain;
detection method
CODCrHJ-828-2017 is adopted for detection, HJ 535-2009 is adopted for ammonia nitrogen detection, and a gravimetric method is adopted for MLSS detection.
Example 1
A tanning wastewater biological synergistic treatment method comprises a mixed strain fermentation step of high-efficiency pollutant degrading bacteria, a lysozyme-producing strain fermentation step and a wastewater treatment step;
the fermentation step of the mixed strain of the high-efficiency pollutant degrading bacteria comprises the following steps:
(1) the cell concentration was adjusted to 6.0X 108Inoculating the cfu/ml inoculation solution into the primary fermentation liquid according to the volume percentage of 10%, and culturing for 30 hours under the conditions that the pH is 7.0, the temperature is 30 ℃ and the ventilation volume is 2.0VVM to prepare primary seed liquid;
the primary fermentation liquid comprises the following components in parts by weight:
cane sugar molasses 2 parts, peptone 0.5 part, K2HPO4·3H2O0.02 part, MgSO40.01 part of FeSO40.01 part and 97 parts of water;
the inoculation liquid contains effective bacteria such as pseudomonas, bacillus licheniformis and bacillus pumilus, and the ratio of the strains of the pseudomonas, the bacillus licheniformis and the bacillus pumilus is 1: 1.5: 1.5;
(2) inoculating the primary seed solution prepared in the step (1) into secondary induction fermentation liquor according to the volume percentage of 10%, and culturing for 16 hours under the conditions that the pH is 7.0, the temperature is 37 ℃ and the ventilation volume is 2.0VVM to obtain mixed bacterial liquid;
the secondary induction fermentation broth comprises the following components in parts by weight:
1 part of active carbon powder with the particle size of 200 meshes, 2 parts of cane sugar molasses, 0.5 part of peptone and K2HPO4·3H2O0.02 part, MgSO40.01 part, FeSO40.01 parts, 25 parts of tannery wastewater and 70 parts of tap water;
the fermentation step of the strain for producing the lysozyme comprises the step of culturing and preparing the strain for producing the lysozyme with the concentration of 6.2 multiplied by 108cfu/ml of bacteriolytic liquid; the method comprises the following specific steps:
(a) inoculating the single strain liquid for producing lysozyme into the primary culture solution according to the proportion of 10 percent by volume, and performing aerobic culture for 28 hours at the temperature of 30 ℃ to prepare a seed solution;
the first-stage culture solution comprises the following components in parts by weight:
corn flour 0.5 parts, cane sugar molasses 0.2 parts, peptone 0.3 parts, NaCl0.3 parts, K2HPO4·3H2O0.02 part, MgSO40.01 part of CaCl20.002 part of (M)nSO40.001 part and 98 parts of water;
(b) inoculating the bacteriolysis seed liquid prepared in the step (a) into a secondary culture solution according to the volume percentage of 10%, and performing aerated culture for 16 hours at the temperature of 30 ℃ to prepare bacteriolysis liquid;
25 parts of tannery wastewater, 1 part of activated carbon powder with the particle size of 200 meshes, 0.5 part of corn flour, 0.2 part of cane sugar molasses, 0.3 part of peptone, 0.3 part of NaCl0.3 part, and K2HPO4·3H2O0.02 part, MgSO40.01 part of CaCl20.002 part of (M)nSO40.001 part and 73 parts of water.
The wastewater treatment steps are as follows:
(i) adjusting the pH value of the tannery wastewater obtained after removing impurities by using a grating to 7.5, and carrying out anoxic treatment for 16h under the condition of an anoxic sludge concentration of 6000mg/L to prepare anoxic-treated wastewater;
(ii) adding the prepared mixed bacterial liquid into the anoxic-treated wastewater prepared in the step (i) according to the continuous adding amount of 5.0mg/L, carrying out aerobic treatment for 32h under the conditions that the concentration of aerobic sludge is 8000mg/L and the dissolved oxygen is 2.0mg/L, and then carrying out mud-water separation to prepare treated water and aerobic sludge;
part of the aerobic sludge prepared in the step (ii) is mixed with the tannery wastewater in the step (i) for recycling; the return flow of the aerobic sludge is 60 percent of the total amount of the aerobic sludge;
(iii) and (3) refluxing 60% of the aerobic sludge prepared in the step (ii) to an anoxic system, allowing the rest 40% of the aerobic sludge to enter a sludge digestion tank, adding a bacteriolytic solution into the sludge digestion tank, wherein the adding amount of the bacteriolytic solution is 30.0mg/L, performing bacteriolytic treatment, separating sludge from water, and mixing the supernatant with the tannery wastewater in the step (i).
Example 2
A tanning wastewater biological synergistic treatment method comprises a mixed strain fermentation step of high-efficiency pollutant degrading bacteria, a lysozyme-producing strain fermentation step and a wastewater treatment step;
the fermentation step of the mixed strain of the high-efficiency pollutant degrading bacteria comprises the following steps:
(1) the cell concentration was adjusted to 6.0X 108Inoculating the cfu/ml inoculation solution into the primary fermentation liquid according to the volume percentage of 10%, and culturing for 48 hours under the conditions that the pH is 8.0, the temperature is 37 ℃ and the ventilation volume is 2.5VVM to prepare primary seed liquid;
the primary fermentation liquid comprises the following components in parts by weight:
sucrose molasses 5 parts, peptone 1 part, K2HPO4·3H2O0.05 part, MgSO40.03 part of FeSO40.03 part and 94 parts of water;
the inoculation liquid contains effective bacteria such as pseudomonas, bacillus licheniformis and bacillus pumilus, and the ratio of the strains of the pseudomonas, the bacillus licheniformis and the bacillus pumilus is 1: 2.5: 2.5;
(2) inoculating the primary seed solution prepared in the step (1) into secondary induction fermentation liquor according to the volume percentage of 5%, and culturing for 24 hours under the conditions that the pH is 8.0, the temperature is 37 ℃ and the ventilation volume is 2.5VVM to obtain mixed bacterial liquid;
the secondary induction fermentation broth comprises the following components in parts by weight:
5 parts of active carbon powder with the particle size of 200 meshes, 5 parts of cane sugar molasses, 1 part of peptone and K2HPO4·3H2O0.05 part, MgSO40.03 part of FeSO40.03 part of tannery wastewater, 40 parts of tannery wastewater and 49 parts of tap water;
the fermentation step of the single strain for producing the lysozyme is to culture and prepare the strain for producing the lysozyme with the concentration of 7.2 multiplied by 108cfu/ml of bacteriolytic liquid; the method comprises the following specific steps:
(a) inoculating the lysozyme-producing single strain bacterial liquid into the primary culture solution according to the volume percentage of 5%, and performing aerobic culture for 48 hours at 37 ℃ to obtain a seed solution;
the first-stage culture solution comprises the following components in parts by weight:
corn flour 0.8 parts, cane sugar molasses 0.5 parts, peptone 1.0 parts, NaCl0.5 parts, K2HPO4·3H2O0.05 part, MgSO40.03 part of CaCl20.005 part, MnSO40.005 part and 97 parts of water;
(b) inoculating the bacteriolysis seed liquid prepared in the step (a) into a secondary culture solution according to the volume percentage of 5%, and performing aeration culture for 24 hours at the temperature of 37 ℃ to prepare bacteriolysis liquid;
40 parts of tannery wastewater, 5 parts of activated carbon powder with the particle size of 200 meshes, 0.8 part of corn flour, 0.5 part of cane sugar molasses, 1.0 part of peptone, 0.5 part of NaCl0.5 part of K2HPO4·3H2O0.05 part, MgSO40.03 part of CaCl20.005 part, MnSO40.005 part and 52 parts of water.
The wastewater treatment steps are as follows:
(i) adjusting the pH value of the tannery wastewater obtained after removing impurities by using a grating to 7.5, and carrying out anoxic treatment for 16h under the condition of an anoxic sludge concentration of 6000mg/L to prepare anoxic-treated wastewater;
(ii) adding the prepared mixed bacterial liquid into the anoxic-treated wastewater prepared in the step (i) according to the continuous adding amount of 15.0mg/L, carrying out aerobic treatment for 32h under the conditions that the concentration of aerobic sludge is 8000mg/L and the dissolved oxygen is 2.0mg/L, and then carrying out mud-water separation to prepare treated water and aerobic sludge;
part of the aerobic sludge prepared in the step (ii) is mixed with the tannery wastewater in the step (i) for recycling; the return flow of the aerobic sludge is 60 percent of the total amount of the aerobic sludge;
(iii) and (3) refluxing 60% of the aerobic sludge prepared in the step (ii) to an anoxic system, allowing the rest 40% of the aerobic sludge to enter a sludge digestion tank, adding a bacteriolytic solution into the sludge digestion tank, wherein the adding amount of the bacteriolytic solution is 30.0mg/L, performing bacteriolytic treatment, separating sludge from water, and mixing the supernatant with the tannery wastewater in the step (i).
Comparative example 1
The process for the biological synergistic treatment of tannery wastewater as in example 1, except that Pseudomonas is replaced with Aeromonas (Aeromonas Salmonicida)
The inoculation liquid contains effective bacteria such as aeromonas, bacillus licheniformis and bacillus pumilus, and the ratio of the strains of the aeromonas, the bacillus licheniformis and the bacillus pumilus is 1: 1.5: 1.5;
the Aeromonas (Aeromonas Salmonicida) is from China general microbiological culture collection center with the culture number of 1.16014;
comparative example 2
The method for biologically and synergistically treating tannery wastewater as described in example 1, wherein Lysobacter xylinum (Lysobacter ruischenii) producing lysozyme is modified to Lysobacter chengduensis
The Lysobacter chengduensis is derived from China general microbiological culture collection center, and has a culture number of 1.15145;
comparative example 3
The method for biologically and synergistically treating tannery wastewater according to example 1, wherein the tannery wastewater in the secondary fermentation liquid component in the steps (2) and (b) is replaced by tap water.
Comparative example 4
The biological synergistic tannery wastewater treatment method as in example 1, except that the activated carbon powder in the step (2) and the step (b) is replaced by diatomite.
Experimental example 1
Selecting a leather-making wastewater treatment plant of Hebeixinji leather production enterprises as an experimental object, wherein the water inlet index is as follows: CODCr1150-1280 mg/L, ammonia nitrogen 124-149 mg/L, pH 7.4-7.6, COD of effluent before synergistic experimentCr220-280 mg/L, and the daily sludge discharge amount (water content is 80%) of ammonia nitrogen of 12-20 mg/L is 15 t/d.
The method of example 1, example 2, comparative example 1, comparative example 2, comparative example 3 and comparative example 4 is respectively adopted to carry out synergistic treatment on the tannery wastewater, and the treatment time of each method is 30 days. Recording the sludge concentration of an anoxic tank and an aerobic tank every day, and controlling the sludge concentration of the anoxic tank to be about 6000mg/L and the sludge concentration of the aerobic tank to be about 8000 mg/L. Daily determination of effluent CODCrAnd recording the sludge discharge amount every day. Taking the water as a system efficiency increasing period 15 days before efficiency increasing, taking the water as a system efficiency increasing stable period 16-30 days before efficiency increasing, and detecting the COD of the effluent water in the stable periodCrValues, ammonia nitrogen values and sludge discharge were averaged and the results are shown in table 1:
TABLE 1
Figure BDA0002058394510000071
Figure BDA0002058394510000081
Analysis of results
As can be seen from the data in table 1, the difference between example 1 and comparative example 1 is only that one of the mixed strains of the high-efficiency pollutant-degrading bacteria is different, which results in a significant difference in the effluent quality, thus demonstrating that the combination of the three bacteria of the present application has a significant technical effect on the treatment of the wastewater, and if any one of the mixed strains is replaced by a similar bacteria, the effect is significantly reduced; in addition, the daily sludge discharge index shows that the subsequent sludge treatment effect is also influenced by the mixed strain collocation of the high-efficiency pollutant degrading bacteria, and the sludge generated by adopting the mixed strain collocation of the application is easier to be digested by lysozyme;
as can be seen from the data in Table 1, the difference between example 1 and comparative example 2 is only that the strains producing lysozyme are different, thereby causing a significant difference in daily sludge discharge, which indicates that the sludge produced by the mixed strain collocation of the application is easier to be digested by lysozyme produced by a specific strain, and has more significant specificity;
as can be seen from the data in Table 1, the difference between example 1 and comparative example 3 is only that the lysozyme producing strain has no acclimation step, resulting in a significant difference between the daily sludge discharge and the water quality data of the treated water, which indicates that the acclimation step has a significant effect on the technical effects of both the wastewater treatment bacteria and the lysozyme producing strain;
as can be seen from the data in Table 1, the difference between example 1 and comparative example 4 is only that the adsorption components are different, so that the daily sludge discharge and the water quality data of the treated water have obvious difference, particularly the index of the ammonia nitrogen in the effluent is obvious, which indicates that the influence of the adsorption components on the removal of the ammonia nitrogen in the wastewater by the mixed strains is obviously higher than that of COD (chemical oxygen demand)CrIndexes prove that the adsorption component active carbon has specificity on the influence of the technical effect.

Claims (8)

1. A tanning wastewater biological synergistic treatment method is characterized by comprising a mixed strain fermentation step of high-efficiency pollutant degrading bacteria, a strain fermentation step of lysozyme production and a wastewater treatment step;
the fermentation step of the mixed strain of the high-efficiency pollutant degrading bacteria comprises the following steps:
(1) inoculating the inoculation liquid into the primary fermentation liquid according to the volume percentage of 5-10%, and culturing for 28-48 hours under the conditions that the pH is 6.5-8.0, the temperature is 30-37 ℃ and the ventilation volume is 1.0-2.5 VVM to prepare primary seed liquid;
the primary fermentation liquid comprises the following components in parts by weight:
2-5 parts of cane sugar molasses, 0.5-1 part of peptone and K2HPO4·3H20.02-0.05 part of O and MgSO40.01 to 0.03 part of FeSO40.01-0.03 part of water and 94-97 parts of water;
the inoculation liquid contains effective bacteria such as pseudomonas, bacillus licheniformis and bacillus pumilus, and the ratio of the strains of the pseudomonas, the bacillus licheniformis and the bacillus pumilus is 1: (1.5-2.5): (1.5-2.5);
in the step (1), Pseudomonas (Pseudomonas pseudorhizoctona) is from China general microbiological culture collection management center, and the strain number is 1.15631; bacillus licheniformis (Bacillus licheniformis) is from China general microbiological culture Collection center with strain number 1.8791; the Bacillus pumilus (Bacillus pumilus) is from China general microbiological culture collection center with the strain number of 1.7456;
(2) inoculating the primary seed solution prepared in the step (1) into secondary induction fermentation liquor according to the volume percentage of 5-10%, and culturing for 16-24 hours under the conditions that the pH is 6.5-8.0, the temperature is 30-37 ℃, and the ventilation volume is 1.0-2.5 VVM, so as to prepare a mixed bacterial solution;
the secondary induction fermentation broth comprises the following components in parts by weight:
1-5 parts of activated carbon powder, 2-5 parts of cane sugar molasses, 0.5-1 part of peptone and K2HPO4·3H20.02-0.05 part of O and MgSO40.01 to 0.03 part of FeSO40.01-0.03 part of tannery wastewater, 25-40 parts of tannery wastewater and 40-70 parts of tap water; the particle size of the activated carbon powder is 200 meshes;
the fermentation step of the single strain for producing the lysozyme is to culture and prepare the strain for producing the lysozyme with the concentration of 5.0 multiplied by 108～1.0×109cfu/ml of bacteriolytic liquid;
the fermentation step of the single strain for producing the lysozyme comprises the following specific steps:
(a) inoculating the lysozyme-producing single strain bacterial liquid into the primary culture solution according to the volume percentage of 5-10%, and performing aerobic culture for 28-48 hours at the temperature of 30-37 ℃ to obtain a bacteriolysis seed liquid;
the first-stage culture solution comprises the following components in parts by weight:
0.5-0.8 part of corn flour, 0.2-0.5 part of cane sugar molasses, 0.3-1.0 part of peptone, 0.3-0.5 part of NaCl, and K2HPO4·3H20.02-0.05 part of O and MgSO40.01 to 0.03 portion of CaCl20.002 to 0.005 part by weight, MnSO40.001-0.005 part of water and 94-98 parts of water;
(b) inoculating the bacteriolytic seed solution prepared in the step (a) into a secondary culture solution according to the volume percentage of 5-10%, and performing aerated culture for 16-24 hours at the temperature of 30-37 ℃ to prepare bacteriolytic liquid;
25-40 parts of tannery wastewater, 1-5 parts of activated carbon powder, 0.5-0.8 part of corn flour, 0.2-0.5 part of cane sugar molasses, 0.3-1.0 part of peptone, 0.3-0.5 part of NaCl, and K2HPO4·3H20.02-0.05 part of O and MgSO40.01 to 0.03 portion of CaCl20.002 to 0.005 part by weight, MnSO40.001-0.005 part of water and 40-75 parts of water; the particle size of the activated carbon powder is 200 meshes;
the wastewater treatment steps are as follows:
(i) adjusting the pH value of the tannery wastewater to 7.5-8.5, and then carrying out anoxic treatment for 16-18 h under the condition of anoxic sludge concentration of 5000-8000 mg/L to prepare anoxic-treated wastewater;
(ii) adding the prepared mixed bacterial liquid into the anoxic-treated wastewater prepared in the step (i) according to the continuous adding amount of 5.0-15.0 mg/L, carrying out aerobic treatment for 32-36 h under the condition of the aerobic sludge concentration of 8000 mg/L-10000 mg/L, and then carrying out sludge-water separation in a sedimentation tank to prepare treated water and aerobic sludge;
(iii) and (3) refluxing 60-70% of the aerobic sludge prepared in the step (ii) to an anoxic system, allowing the rest 30-40% of the aerobic sludge to enter a sludge digestion tank, adding a bacteriolytic solution into the sludge digestion tank, wherein the addition amount of the bacteriolytic solution is 15.0-30.0 mg/L, performing bacteriolytic treatment, performing sludge-water separation, and mixing the supernatant with the tannery wastewater in the step (i).
2. The method according to claim 1, wherein in the step (1), the concentration of the cells in the inoculum is 5.0108～8.0×108cfu/ml。
3. The process of claim 1, wherein the single strain of lysozyme is Lysobacter xylinum (Lysobacter ruischeni) with strain number 1.10136, which is derived from the China center for general microbiological culture Collection.
4. The treatment process according to claim 1, wherein the tannery wastewater is obtained by removing impurities through a grid.
5. The process of claim 1, wherein a portion of the aerobic sludge precipitated in step (ii) is returned to the anoxic system.
6. The treatment method according to claim 5, wherein the amount of the aerobic sludge recycled is 60 to 70% of the total amount of the aerobic sludge.
7. The treatment method according to claim 1, wherein the amount of dissolved oxygen in step (ii) is 2.0 to 4.0 mg/L.
8. The treatment method according to claim 1, wherein the aerobic sludge added with the lysozyme in the step (iii) accounts for 30-40% of the total amount of the aerobic sludge.
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