CN101260373A - Micrococcus luteus with novel metabolic characteristic and application thereof in biological degradation - Google Patents
Micrococcus luteus with novel metabolic characteristic and application thereof in biological degradation Download PDFInfo
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- CN101260373A CN101260373A CN 200710157803 CN200710157803A CN101260373A CN 101260373 A CN101260373 A CN 101260373A CN 200710157803 CN200710157803 CN 200710157803 CN 200710157803 A CN200710157803 A CN 200710157803A CN 101260373 A CN101260373 A CN 101260373A
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Abstract
The invention relates to a gamboges micrococcus provided with novel metabolism characteristics and application of the gamboges micrococcus in biodegradation, belonging to the bioengineering technical field. The invention produces a gamboges micrococcus provided with the novel metabolism characteristics by means of separation sieve. The invention has the advantages that: the gamboges micrococcus degrades nitrobenzene by means of partial reduction and mineralizes by-products - picolinic acids during the degradation process, thereby complete degradation of the nitrobenzene is realized and the problem during the process of partial reduction degradation of the nitrobenzene is solved; growth of the gamboges micrococcus under the condition of high salinity is not affected, thereby the nitrobenzene is completely degraded; the gamboges micrococcus can take the picolinic acids as a single carbon source, a single nitrogen source and a single energy source for growth, and the picolinic acids are finally mineralized into innoxious carbon dioxides and water; the gamboges micrococcus can be used as biological enhancing preparations and applied in biological treatment of nitrobenzene waste water and picolinic acid waste water with high salinity.
Description
Technical field
The invention belongs to technical field of bioengineering, relate to micrococcus luteus and the application in oil of mirbane aerobic biodegradation and pyridine carboxylic acid aerobic biodegradation thereof that a strain has new metabolic characteristics.
Background technology
Oil of mirbane is widely used in the production of medicine, dyestuff, plastics, explosive etc., and the widespread use of oil of mirbane causes the nitrobenzene contamination environment.Nitrobenzene waste water can be handled by aerobe method.At present, the Microbial resources of aerobic degradation oil of mirbane have bacterium and whiterot fungi, and bacterium has pseudomonas, comamonas, bacillus, coryneform bacteria, staphylococcus, suis etc.
Actual oil of mirbane trade effluent has characteristics such as salinity height, complicated component.High salinity waste water is meant that total dissolved solid thing (sodium-chlor) is at least the waste water of 3.5% (W/V).Under high salt condition, the nitrobenzene degradation bacterium can not grow usually, therefore can not realize the degraded of oil of mirbane.
The nitrobenzene degradation bacterium of being reported at present passes through two kinds of approach degrading nitrobenzenes under aerobic condition: oxidative pathway or partial reduction approach, wherein partial reduction approach is more extensive.In isolating 155 strain bacterium such as Spain, 154 strains are by partial reduction approach degrading nitrobenzene, and 1 strain is by the oxidative pathway degrading nitrobenzene.This is because the sucting electronic effect of nitro causes cloud density decline on the phenyl ring, thereby has hindered the electric attack of oxidasic parent; Simultaneously, the nitro electronegativity strengthens, is easy to be reduced.Self structure just because of oil of mirbane causes present institute strain separated nearly all by partial reduction approach degrading nitrobenzene.Yet in the partial reduction degraded of oil of mirbane, pyridine carboxylic acid generates as by product, and pyridine carboxylic acid has bio-toxicity, can not be utilized by the nitrobenzene degradation bacterium, and the generation of pyridine carboxylic acid has hindered the aerobic degradation of oil of mirbane.
Pyridine carboxylic acid is a kind of N heterocyclization and thing, is mainly used in the production of medicine, agricultural chemicals, dyestuff etc., and the application of pyridine carboxylic acid causes the pyridine carboxylic acid contaminate environment.At present, the Microbial resources of aerobic degradation pyridine carboxylic acid have Arthrobacter and mycobacterium.
Summary of the invention
Purpose of the present invention is exactly this problem of by product pyridine carboxylic acid that can not degrade to occur at the high characteristics of actual oil of mirbane trade effluent salinity and in oil of mirbane partial reduction process, tame, filter out the oil of mirbane efficient degrading bacteria by scientific approach, in order to the high nitrobenzene waste water of processing salinity, and the bottleneck in the solution nitrobenzene degradation process.Simultaneously, also provide efficient, practical Microbial resources for the aerobic biodegradation of pyridine carboxylic acid.
Technical solution of the present invention is, micrococcus luteus provided by the invention is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center "; its preserving number CGMCCNo.1760, classification called after Micrococcus luteus on July 18th, 2006.Address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, postcode: 100080.
Described micrococcus luteus, its concrete screening step is as follows: get the 50ml active sludge from Dalian Dye Factory aeration tank, the trisodium phosphate of adding 0.2% after vibration 5-10min smashes the active sludge flco on the vibrator, is got the 5ml active sludge as separating flocculation agent, adding contains shaking in the bottle of 95ml minimal medium, add oil of mirbane again as sole carbon, nitrogen, the energy, wherein the concentration of oil of mirbane is 200mg/L, carries out shaking table and cultivates, 30 ℃ of temperature, rotating speed 180r/min; When bacterium liquid becomes muddy, transfer, obtaining with oil of mirbane is the stable bacterium liquid of sole carbon, nitrogen, the energy; To stablize bacterium liquid dilution, on the solid medium that with oil of mirbane is sole carbon, nitrogen, the energy, be coated with, this solid medium will be put into illumination box, 30 ℃, cultivated 4-5 days, choose single bacterium colony; Isolate a strain and be with oil of mirbane sole carbon, nitrogen, the energy micrococcus luteus.
Described micrococcus luteus is characterized in that, this strain growth appropriate pH scope is 6.0-9.5, and the suitable growth temperature scope is 20-35 ℃; Its morphological specificity: on the LB agar slant, cultivate after 4-5 days down for 25 ℃, form level and smooth, convex surface, bacterium colony that the edge is complete, diameter 1-2mm, yellow is not had a reactivity, do not produce Endospore, the appearance of bacterium colony is not because of the aging of bacterium or the difference of external environment or substratum changes to some extent, and bacterial strain is the spherical thallus of diameter 0.9-1.8 μ m, is one or the irregular shape growth of growing thickly with 4.
The application of described micrococcus luteus in the oil of mirbane aerobic biodegradation, it is characterized in that, micrococcus luteus immobilized cell, cell liquid culture are all by partial reduction approach degrading nitrobenzene, and the by product pyridine carboxylic acid in the mineralizing and degrading process, realize the thorough degraded of oil of mirbane, therefore can handle as biological reinforced preparation p-nitrophenyl waste water.
The application of described micrococcus luteus in the oil of mirbane aerobic biodegradation, it is characterized in that, micrococcus luteus immobilized cell, cell liquid culture are all under the condition of NaCl mass concentration 0-50mg/L, thoroughly therefore degrading nitrobenzene can be handled the nitrobenzene waste water of high salinity as biological reinforced preparation.
The application of described micrococcus luteus in the pyridine carboxylic acid aerobic biodegradation, it is characterized in that, micrococcus luteus immobilized cell, cell liquid culture all destroy the N heterocycle of pyridine carboxylic acid, therefore pyridine carboxylic acid open loop mineralising can be handled pyridine carboxylic acid waste water as biological reinforced preparation.
Described micrococcus luteus physiological and biochemical property sees Table 1
Table 1 micrococcus luteus physiological and biochemical property
Annotate :+: the positive;-: feminine gender
Described micrococcus luteus both can be grown in eutrophy substratum LB, also can grow in small organic molecules such as glucose, sucrose, starch are the minimal medium of sole carbon source.
Described micrococcus luteus can be with the bacterial classification or make immobilized cell p-nitrophenyl waste water and pyridine carboxylic acid waste water biodegrade in vegetative period of fresh culture.
The beneficial effect that the present invention reached is: micrococcus luteus provided by the invention is by partial reduction approach degrading nitrobenzene, and the by product pyridine carboxylic acid in the mineralizing and degrading process, thereby realize the thorough degraded of oil of mirbane, solved the bottleneck in the oil of mirbane partial reduction degraded; Micrococcus luteus is thorough degrading nitrobenzene under the condition of sole carbon, nitrogen, the energy and oil of mirbane starting point concentration≤200mg/L at oil of mirbane; Micrococcus luteus is at NaCl mass concentration 0-50mg/L, and oil of mirbane is thorough degrading nitrobenzene under the condition of sole carbon, nitrogen, the energy and oil of mirbane starting point concentration≤200mg/L; Micrococcus luteus is thorough degraded pyridine carboxylic acid under the condition of sole carbon, nitrogen, the energy and pyridine carboxylic acid starting point concentration≤2000mg/L at pyridine carboxylic acid; Micrococcus luteus provided by the invention can be used as biological bacteria preparation, is added in existing nitrobenzene waste water and the pyridine carboxylic acid Waste Water Treatment, strengthens the processing power of former processing system; Micrococcus luteus provided by the invention has wide application potential in the processing of nitrobenzene waste water and pyridine carboxylic acid waste water.
Description of drawings
Fig. 1 is a micrococcus luteus provided by the invention is being that sole carbon, nitrogen, the energy and oil of mirbane starting point concentration are growth and nitrobenzene degradation graphic representation in the liquid culture of 200mg/L with oil of mirbane, the left side ordinate zou is represented the rate of recovery of oil of mirbane among the figure, unit is %, the right side ordinate zou is represented dry cell weight, and unit is mg/L;
Among the figure :-■-the represent rate of recovery ,--represent thalli growth amount.
Fig. 2 is a micrococcus luteus provided by the invention is being that sole carbon, nitrogen, the energy and pyridine carboxylic acid starting point concentration are growth and pyridine carboxylic acid degradation curve figure in the liquid culture of 2000mg/L with the pyridine carboxylic acid, the left side ordinate zou is represented the rate of recovery of pyridine carboxylic acid among the figure, unit is %, the right side ordinate zou is represented the increment of thalline, and unit is OD
660
Among the figure :-■-the represent rate of recovery ,--represent thalli growth amount.
Fig. 3 is that micrococcus luteus provided by the invention is dense down in different sodium-chlor quality, oil of mirbane is that sole carbon, nitrogen, the energy and oil of mirbane starting point concentration are when being 200mg/L, the degradation curve figure of oil of mirbane, ordinate zou is represented the rate of recovery of oil of mirbane among the figure, unit is %;
Among the figure :-■-represent sodium-chlor mass concentration 20mg/L ,--represent sodium-chlor mass concentration 30mg/L ,-zero-represent sodium-chlor mass concentration 50mg/L ,-●-represent sodium-chlor mass concentration 70mg/L ,-△-represent sodium-chlor mass concentration 100mg/L.
Among Fig. 1 to 3: X-coordinate h all represents the time, and unit is hour.
Embodiment
The invention will be further described below in conjunction with accompanying drawing.
Embodiment 1
Micrococcus luteus provided by the invention, its screening step is as follows:
From the aeration tank of Dalian Dye Factory, gather the aerobic activated sludge sample, tame, cultivate as the bacterium source.With the 50ml active sludge, the trisodium phosphate of adding 0.2% is as separating flocculation agent, after vibration 5-10min smashes the active sludge flco on the vibrator, get the 5ml active sludge, adding contains shaking in the bottle of 95ml minimal medium, adds oil of mirbane again as sole carbon, nitrogen, the energy, and wherein the concentration of oil of mirbane is 200mg/L, carry out shaking table and cultivate 30 ℃ of temperature, rotating speed 180r/min.Minimal medium composition: Na
2HPO
412H
2O, 7g/L; KH
2PO
4, 1g/L; CaCl
22H
2O, 10mg/L; FeCl
3, 2mg/L; MgSO
47H
2O, 20mg/L.When bacterium liquid becomes muddy, oil of mirbane detect less than, transfer next time; Cultivate through domestication constantly, finally obtaining with oil of mirbane is the stable bacterium liquid of sole carbon, nitrogen, the energy.To stablize the dilution of bacterium liquid, on the solid medium that with oil of mirbane is sole carbon, nitrogen, the energy, be coated with.Solid culture based component: Na
2HPO
412H
2O, 7g/L; KH
2PO
4, 1g/L; CaCl
22H
2O, 10mg/L; FeCl
3, 2mg/L; MgSO
47H
2O, 20mg/L; Oil of mirbane, 200mg/L, agar 2%.This solid medium is put into illumination box, 30 ℃, cultivated 4-5 days, choose single bacterium colony; Separating a strain is the micrococcus luteus of sole carbon, nitrogen, the energy with oil of mirbane; And this bacterial strain is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number CGMCC NO.1760 on July 18th, 2006.
The appropriate pH value of this micrococcus luteus is 7.0, is fit to growth temperature at 25 ℃; Its morphological specificity is on the LB agar slant, cultivate after 4-5 days down for 25 ℃, form level and smooth, convex surface, bacterium colony that the edge is complete, diameter 1-2mm, yellow is not had a reactivity, do not produce Endospore, the appearance of bacterium colony is not because of the aging of bacterium or the difference of external environment or substratum changes to some extent, and bacterial strain is the spherical thallus of diameter 0.9-1.8 μ m, is one or the irregular shape growth of growing thickly with 4.
The 16S rDNA amplification of micrococcus luteus provided by the invention:
By the 16S rDNA of this bacterial strain that increases, having obtained length is the 16S rDNA D1/D2 sequence of 1433bp.The PCR primer adopts universal primer 27f (5 '-GAGTTTGATC (AC) TGGCTCAG-3 ') and the 1492r (5 '-TACGG (CT) TACCTTGTTACGACTT-3 ') of 16S rDNA.(GeneAmp, PCR System9700) carries out amplified reaction with the PCR instrument.The reaction system cumulative volume is 25 μ l: ultrapure water 16.2 μ l, and 10xBuffer 2.5 μ l, dNTP 2 μ l, F8 primer 1 μ l, R22 primer 1 μ l, ExTaq enzyme 0.3 μ l, total DNA do to get 2 μ l in reaction system after certain dilution.94 ℃ of pre-sex change 5min, through 30 circulations, wherein, and 98 ℃ of sex change 20s, 55 ℃ of annealing 40s, 72 ℃ are extended 1.5min, extend 7min at 72 ℃ again after 30 circulations, 1% agarose gel electrophoresis, EB dyeing back ultraviolet detection.Carry out homology relatively by the Blast program, the homology that shows this bacterial strain and Micrococcus luteus is up to 99%.
The cell liquid culture of preparation micrococcus luteus:
Picking is the micrococcus luteus list bacterium colony on the solid medium of sole carbon, nitrogen, the energy with oil of mirbane, what sterilization was housed is in the liquid nutrient medium of sole carbon, nitrogen, the energy with oil of mirbane, wherein the concentration of oil of mirbane is 100mg/l, in 30 ℃, pH 7.0,180r/min, carry out aerobic cultivation 100-120 hour, getting cultured bacterium liquid 1ml is seeded in the minimal medium of 100ml nitrobenzene-containing (200mg/l), 30 ℃, pH 7.0,180r/min, carry out aerobic cultivation 120 hours, obtain the cell liquid culture of micrococcus luteus.
The immobilized cell of preparation micrococcus luteus:
[1] adopts the surface adsorption immobilization technology;
[2] patented product of selecting for use Dalian Landa Biological Environmental Technology Co., Ltd. to provide, DW-22 type macroporous netlike carrier is as immobilization material, and table 2 has provided the performance of carrier;
Table 2DW-22 type macroporous netlike carrier property
[3] add the 100ml minimal medium in the 250ml Erlenmeyer flask, add oil of mirbane again as sole carbon, nitrogen, the energy, wherein the concentration of oil of mirbane is 200mg/l;
[4] DW-22 type carrier is directly added in the Erlenmeyer flask of above-mentioned [3], 121 ℃ of 20min that sterilize down, the carrier dosage is 24% (volume ratio), wherein the carrier dosage is the carrier bulk of adding and the ratio of nutrient solution volume;
[5] the cell liquid culture of inoculation 1ml micrococcus luteus in 30 ℃, pH 7.0,160r/min, carries out aerobic cultivation;
[6] when oil of mirbane is degraded fully, adhered to a large amount of micrococcus luteus cells on the macroporous netlike carrier, micrococcus luteus is fixed on the macroporous netlike carrier;
[7] adhered to the macroporous netlike carrier of micrococcus luteus cell with aseptic normal saline flushing, washed altogether 3 times, this macroporous netlike carrier has been immersed in the physiological saline, 4 ℃ of storages, standby.
Embodiment 2
Among the present invention, the application of micrococcus luteus p-nitrophenyl aerobic degradation research, its step is as follows:
[1] add the 100ml minimal medium in the 250ml Erlenmeyer flask, the oil of mirbane that adds 200mg/L is again sterilized down for 121 ℃ as sole carbon, nitrogen, the energy;
[2] the micrococcus luteus cell liquid culture with embodiment 1 preparation adds in the substratum of above-mentioned [1], 30 ℃, 180r/min, and aerobic cultivation, Fig. 1 is the growth and the nitrobenzene degradation situation of micrococcus luteus.
Embodiment 3
Among the present invention, micrococcus luteus is to the application of pyridine carboxylic acid aerobic degradation research, and its step is as follows:
[1] add the 100ml minimal medium in the 250ml Erlenmeyer flask, the pyridine carboxylic acid that adds 2000mg/L is sterilized down for 121 ℃ as sole carbon, nitrogen, the energy;
[2] the micrococcus luteus cell liquid culture with embodiment 1 preparation adds in the substratum of above-mentioned [1], 30 ℃, 180r/min, and aerobic cultivation, Fig. 2 is the growth and the pyridine carboxylic acid degraded situation of micrococcus luteus.
Embodiment 4
Among the present invention, the application of micrococcus luteus p-nitrophenyl aerobic degradation research under high salinity, its step is as follows:
[1] preparing the sodium-chlor mass concentration respectively is 2%, 3%, 5%, 7% and 10% minimal medium, and the oil of mirbane that adds 200mg/L is as sole carbon, nitrogen, the energy, 121 ℃ of sterilizations down;
[2] the micrococcus luteus cell liquid culture with embodiment 1 preparation adds in the substratum of above-mentioned [1], and 30 ℃, 180r/min, aerobic cultivation 120h, Fig. 3 are growth and the nitrobenzene degradation situation of micrococcus luteus under different salinity.
Embodiment 5
Among the present invention, the application of micrococcus luteus immobilized cell p-nitrophenyl aerobic degradation research, its step is as follows:
[1] liquid nutrient medium of configuration embodiment 4 described different salinity;
[2] embodiment 1 described micrococcus luteus immobilized cell is added in the substratum of above-mentioned [1] 30 ℃, 160r/min, aerobic cultivation respectively; Table 3 has been listed the degraded of micrococcus luteus immobilized cell p-nitrophenyl under different salinity, be under 7% the condition in salinity, the degradation rate of micrococcus luteus immobilized cell 120h p-nitrophenyl is 59.33%, and the degradation rate of micrococcus luteus cell liquid culture 120h p-nitrophenyl is 38.53%; The result shows with the cell liquid culture and compares that the salt tolerance of immobilized cell strengthens to some extent.
The degraded of table 3 micrococcus luteus immobilized cell p-nitrophenyl under different salinity
Embodiment 6
Among the present invention, the micrococcus luteus immobilized cell is to the application of pyridine carboxylic acid aerobic degradation research, and its step is as follows:
[1] in the 250ml Erlenmeyer flask, adds the 100ml minimal medium, add the pyridine carboxylic acid of 2000mg/L, 121 ℃ of sterilizations down;
[2] the micrococcus luteus immobilized cell with embodiment 1 preparation adds in the substratum of above-mentioned [1], 30 ℃, 160r/min, and aerobic cultivation, degrade fully time of pyridine carboxylic acid of immobilized cell is 60h.
＜110〉Dalian University of Technology's environment and life institute
＜120〉have the micrococcus luteus of new metabolic characteristics and the application in biological degradation thereof
＜160〉the sequence sum 1
<211>1433bp
<212>DNA
＜213〉micrococcus luteus (Micrococcus luteus)
<220>
<221>gene
＜120〉micrococcus luteus 16S rDNA sequence
1 ggtcggcggc?gtgcttacca?tgcagtcgaa?cgatgaagcc?cagcttgctg?ggtggattat
61 ggcgaacggg?tgagtaacac?gtgagtaacc?tgcccttaac?tctgggataa?gcctgggaaa
121 ctgggtctaa?taccggatag?gagcgcctac?cgcatggtgg?gtgttggaaa?gatttatcgg
181 ttttggatgg?actcgcggcc?tatcagcttg?ttggtgaggt?aatggctcac?caaggcgacg
241 acgggtagcc?ggcctgagag?ggtgaccggc?cacactggga?ctgagacacg?gcccagactc
301 ctacgggagg?cagcagtggg?gaatattgca?caatgggcgc?aagcctgatg?cagcgacgcc
361 gcgtgaggga?tgacggcctt?cgggttgtaa?acctctttca?gtagggaaga?agcgaaagtg
421 acggtacctg?cagaagaagc?accggctaac?tacgtgccag?cagccgcggt?aatacgtagg
481 gtgcgagcgt?tatccggaat?tattgggcgt?aaagagctcg?taggcggttt?gtcgcgtctg
541 tcgtgaaagt?ccggggctta?accccggatc?tgcggtgggt?acgggcagac?tagagtgcag
601 taggggagac?tggaattcct?ggtgtagcgg?tggaatgcgc?agatatcagg?aggaacaccg
661 atggcgaagg?caggtctctg?ggctgtaact?gacgctgagg?agcgaaagca?tggggagcga
721 acaggattag?ataccctggt?agtccatgcc?gtaaacgttg?ggcactaggt?gtggggacca
781 ttccacggtt?tccgcgccgc?agctaacgca?ttaagtgccc?cgcctgggga?gtacggccgc
841 aaggctaaaa?ctcaaaggaa?ttgacggggg?cccgcacaag?cggcggagca?tgcggattat
901 ttcgatgcaa?cgcgaagaac?cttaccaagg?cttgacatgt?tctcgatcgc?cgtagagata
961 cggtttcccc?tttggggcgg?gttcacaggt?ggtgcatggt?ggtcgtcagc?tcgtgtcgtg
1021?agatgttggg?ttaagtcccg?caacgagggc?aaccctcgtt?ccatgttgcc?agcacgtagt
1081?ggtggggatt?catgggagac?tgccggggtc?aactcggagg?aaggtgagga?ggacgtcaaa
1141?tcatcatgcc?ccttatgtct?tgggcttcac?gcatgctaca?atggccggta?caatgggttg
1201?ggatactgtg?aggtggagct?aatcccaaaa?agccggtctc?agttcggatt?ggggtctgca
1261?actcgacccc?atgaagtcgg?agtcgctagt?aatcgcagat?cagcaacgct?gcggtgaata
1321?cgttcccggg?ccttgtacac?accgcccgtc?aagtcacgaa?agtcggtaac?acccgaagcc
1381?ggtggcctaa?cccttgtggg?aggagccgtc?gaaggtgtac?ctgcgagaca?ctc
Claims (5)
1, has the micrococcus luteus of new metabolic characteristics and the application in biological degradation thereof, it is characterized in that, micrococcus luteus is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center "; preserving number CGMCC No.1760, classification called after Micrococcus luteus on July 18th, 2006.
2, according to claim 1 have the micrococcus luteus of new metabolic characteristics and an application in biological degradation thereof, it is characterized in that, from Dalian Dye Factory aeration tank, get the 50ml active sludge, the trisodium phosphate of adding 0.2% is as separating flocculation agent, after vibration 5-10min smashes the active sludge flco on the vibrator, get the 5ml active sludge, adding contains shaking in the bottle of 95ml minimal medium, add oil of mirbane again as sole carbon, nitrogen, the energy, wherein the concentration of oil of mirbane is 200mg/L, carry out shaking table and cultivate 30 ℃ of temperature, rotating speed 180r/min; When bacterium liquid becomes muddy, transfer next time, obtaining with oil of mirbane is the stable bacterium liquid of sole carbon, nitrogen, the energy; To stablize bacterium liquid dilution, on the solid medium that with oil of mirbane is sole carbon, nitrogen, the energy, be coated with, this solid medium will be put into illumination box, 30 ℃, cultivated 4-5 days, choose single bacterium colony; Isolate a strain and be with oil of mirbane sole carbon, nitrogen, the energy micrococcus luteus.
3, claim 1 is described has the micrococcus luteus of new metabolic characteristics and an application in biological degradation thereof, it is characterized in that when oil of mirbane was sole carbon, nitrogen, the energy, the maximum tolerated concentration of micrococcus luteus p-nitrophenyl reached 200mg/L; The total clearance of TOC reaches 98.26%; Micrococcus luteus can be degraded as the waste water of biological reinforced preparation p-nitrophenyl concentration≤200mg/L.
4, claim 1 is described has the micrococcus luteus of new metabolic characteristics and an application in biological degradation thereof, it is characterized in that, when oil of mirbane is that sole carbon, nitrogen, the energy and sodium chloride concentration are 0-50mg/L, micrococcus luteus is degrading nitrobenzene thoroughly; Micrococcus luteus can be degraded to the waste water of salinity≤50mg/L, nitro phenenyl concentration≤200mg/L as biological reinforced preparation.
5, claim 1 is described has the micrococcus luteus of new metabolic characteristics and an application in biological degradation thereof, it is characterized in that, when pyridine carboxylic acid is sole carbon, nitrogen, the energy, micrococcus luteus reaches 2000mg/L to the maximum tolerated concentration of pyridine carboxylic acid; The total clearance of TOC reaches 99.17%; Micrococcus luteus can be degraded to the waste water of pyridine carboxylic acid concentration≤2000mg/L as biological reinforced preparation.
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| CN101955256A (en) * | 2010-09-19 | 2011-01-26 | 东华大学 | Method for preparing ibuprofen-degrading bacterial agent |
| CN101955256B (en) * | 2010-09-19 | 2013-01-16 | 东华大学 | Method for preparing ibuprofen-degrading bacterial agent |
| CN104894020A (en) * | 2015-05-30 | 2015-09-09 | 中国海洋大学 | Protease and production strain thereof |
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| CN105540997A (en) * | 2015-12-12 | 2016-05-04 | 常州大学 | Method for treating nitrobenzene wastewater |
| CN107653197A (en) * | 2017-11-13 | 2018-02-02 | 深圳市长隆科技有限公司 | A kind of preparation method of microorganism formulation and its application in leather waste water processing |
| CN107653197B (en) * | 2017-11-13 | 2021-06-22 | 深圳市长隆科技有限公司 | Preparation method of microbial preparation and application of microbial preparation in leather wastewater treatment |
| CN113583918A (en) * | 2021-09-03 | 2021-11-02 | 佛山市玉凰生态环境科技有限公司 | River sediment degrading strain and application thereof |
| CN114621904A (en) * | 2022-05-13 | 2022-06-14 | 中国科学院地理科学与资源研究所 | Micrococcus and application thereof |
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