CN106746155A - A kind of processing method of Lincomycin wastewater - Google Patents
A kind of processing method of Lincomycin wastewater Download PDFInfo
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Abstract
The present invention relates to a kind of processing method of Lincomycin wastewater, including（1）Lincomycin wastewater is pre-processed using high-level oxidation technology；（2）Coagulating sedimentation, the precipitation that filtering removal is produced are carried out to oxidation water outlet；（3）Anaerobic biochemical treatment is carried out to water outlet after filtering；（4）Anaerobic effluent is processed using biological contact oxidation process, while adding the de- COD bacterium of salt tolerant, the de- COD bacterium of the salt tolerant are secondary coccus（Paracoccus sp.）FSTB-2 and/or Pseudomonas stutzeri（Pseudomonas stutzeri）FSTB-5, is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, and deposit number is CGMCCNo.10938, CGMCCNo.10940.The present invention uses advanced oxidation-coagulating sedimentation-anaerobic biological-catalytic oxidation handling process, and add the de- COD bacterium of specific salt tolerant in catalytic oxidation unit, it is resistant to the antibiotic in waste water, the efficient stable removing of COD in waste water is realized, with process is simple, treatment effeciency be high, the low feature of processing cost.
Description
Technical field
The invention belongs to technical field of waste water processing, and in particular to a kind of processing method of Lincomycin wastewater.
Background technology
Antibiotic is the great discovery in human history, is widely used for the multiple fields such as medical treatment, effective guarantee human body health.But in antibiotics production and application process, the substantial amounts of organic wastewater with difficult degradation thereby containing antibiotic can be produced.Lincomycin is that a class may act on the ribosomal 50S subunits of sensitive bacteria, prevent the extension of peptide chain, so as to the antibiotic that the protein for suppressing bacterial cell synthesizes, low concentration lincomycin is commonly used for bacteriostatic agent, can be used as the bactericide of the height sensitive bacterials such as most of gram-positive bacterias and some anaerobic bacterias in higher concentrations, therefore microorganism to biochemical processing process has certain inhibitory action.Lincomycin production of raw medicine waste water is mainly derived from remaining raffinate after broth extraction, and main component is carbohydrate, protein, class ester thing, and also a small amount of antibiotic and solvent etc., COD are 14000-18000mg/L, and pH value is in alkalescence.Lincomycin wastewater has the features such as yield is big, organic concentration is high, colourity is deep, inorganic salt content is high, complicated component, biodegradability are poor, and because the self-purification ability of enviroment is limited, its discharge cause severe contamination to meeting to environment.
At present, to Lincomycin wastewater frequently with anaerobic biochemical and aerobic biochemical process combined treatment technology.
Anaerobic biochemical is proved to be a kind of method of effective lifting waste water biodegradability, and conventional anaerobic biological technology has anaerobic baffled reactor（ABR）, upflow anaerobic sludge blanket reactor（UASB）, hydrolysis acidification method, internal-circulation anaerobic reactor（IC）Deng.Fang Lingling etc.（Using the research [J] of anaerobic baffled reactor Pharmaceutical Wastewater Treatment, Anhui chemical industry, 2006,2）Lincomycin pharmacy waste water is processed using anaerobic baffled reactor, the biodegradability of the waste water is effectively improved.Deng Liling etc.（Biochemical process processes technical study [J] water technologies of Lincomycin wastewater, 2008,34 (4):23-25）Using in internal-circulation anaerobic reactor（IC）Inoculation 0.3%-0.4% effective microbes（EM）Mode Lincomycin wastewater COD can be processed to below 300mg/L, treatment effect need further raising.
Used as a kind of organic matter degradation removing sulfuldioxide, it can be effectively to process the COD in waste water that it is applied in combination with anaerobic biochemical to aerobic biochemical process.Conventional aerobic biochemical process has sequencing batch active sludge（SBR）, biological contact oxidation process, loop cycle activated Sludge System（CASS）And membrane bioreactor（MBR）Deng.
Shi Jijun etc.（Application [J] of the UASB-SBR methods in lincomycin production of raw medicine waste water, pharmaceutical engineering design magazine, 200,23 (4)）It is the lincomycin production of raw medicine waste water of 14000-18000mg/L to use UASB-SBR methods to process COD concentration, and SBR water outlets COD can be down to 280mg/L.Ling Xiao（Two-stage ABR+ flocculation sediments+A/O processes the technical study [D] of high-concentration sulfuric acid salt organic waste water, University Of Nanchang, 2010,6）The high-concentration sulfuric acid salt organic waste water that lincomycin and Chinese caterpillar fungus powder are produced is produced using ABR1+ flocculation sediment+ABR2+A/O PROCESS FOR TREATMENTs, ABR1 acclimation periods 90 days, sulfate clearance 90%, COD clearances 30%, ABR2 acclimation periods 75 days, COD clearances 68%, A/O effluent COD concentrations are down to 280mg/L.Niu Na（Lincomycin waste water treatment process and startup research [D], Zhengzhou University, 2010,5）Lincomycin wastewater is processed using IC+A/O group technologies, IC sections of COD can be down to the 2450mg/L of water outlet by the 11000mg/L intake, and A/O sections of COD can be down to 612mg/L of water outlet or so by the 2450mg/L intake.Qu Xiuhua etc.（The research [J] of lincomycin wastewater treatment, water technology, 2009,35 (4):69-73）Using internal-circulation anaerobic reactor（IC）, loop cycle activated Sludge System（CASS）And membrane bioreactor（MBR）Treatment Lincomycin wastewater, influent quality is COD17800mg/L, ammonia nitrogen 568mg/L, pH value 8.5-9.2, and water outlet COD clearances are up to 90% or so.Shen little Hua etc.（Antibiotics Wastewater Treatment [J], water technology, 2009,10）Using hydrolysis acidification-inner circulation reactor-circulating active sludge processing system-air-float technology treatment lincomycin hydrochloride production waste water, stable water outlet is processed, COD is up to 180mg/L.Wang Bing（Lincomycin Technology of High Concentration OrganicWastewater Treatment [J], fwaater resources protection, 2008,24 (4):53-57）Using internal-circulation anaerobic reactor（IC）And intermittent activated sludge process（SBR）To COD concentration for the Lincomycin wastewater of 6000-9000mg/L carries out continuous processing, two sections of COD clearances can respectively reach 91% and 61% to combination technique, and water outlet COD is less than 300mg/L.
The above method is processed using anaerobic-aerobic group technology mostly, and Anaerobic Treatment can remove part COD with the biodegradability of lifting waste water；Aerobic Process for Treatment then continues to carry out oxidative degradation to the pollutant in waste water.By after combined treatment, COD is still above 200mg/L, after also needing to be diluted treatment with clear water or recirculated water, emission request could be met, increased the processing cost of unit waste water, be unfavorable for energy-saving and emission-reduction.Additionally, waste water salt content is higher also to produce certain inhibitory action to anaerobic and aerobic microorganism, the problems such as difficult stably reaching standard discharge, technique can not be run steadily in the long term in the such as long and short phase in process debugging cycle occurs during actual treatment process operation.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of processing method of Lincomycin wastewater.The present invention uses advanced oxidation-coagulating sedimentation-anaerobic biological-catalytic oxidation handling process, and add the de- COD bacterium of specific salt tolerant in catalytic oxidation unit, it is resistant to the antibiotic in waste water, COD efficient stables removing in waste water is realized, with process is simple, treatment effeciency be high, the low feature of processing cost.
The processing method of Lincomycin wastewater of the present invention, comprises the following steps：
（1）Advanced oxidation treatment：Lincomycin wastewater is pre-processed using high-level oxidation technology；
（2）Coagulating kinetics：Coagulating sedimentation, the precipitation that filtering removal is produced are carried out to oxidation water outlet；
（3）Anaerobic biochemical treatment：To step（2）Water outlet carries out anaerobic biochemical treatment after filtering；
（4）Biological reinforced treatment：Anaerobic effluent is processed using biological contact oxidation process, while adding the de- COD bacterium of salt tolerant, the de- COD bacterium of the salt tolerant are secondary coccus（Paracoccus sp.）FSTB-2 and/or Pseudomonas stutzeri（Pseudomonas stutzeri）FSTB-5, is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, and deposit number is CGMCCNo.10938, CGMCCNo.10940.
Lincomycin wastewater of the present invention is mainly derived from technique waste water and washes during lincomycin production of raw medicine etc., and main component is carbohydrate, protein, class ester thing, also a small amount of antibiotic and solvent etc..Waste water quality is：COD（Chromium method, similarly hereinafter）It is 6000-18000mg/L, salt content is 0.1wt%-2.5wt%, and suspension is 500-5000mg/L, and ammonia nitrogen concentration is 5-200mg/L, pH value 7.5-10.
Step of the present invention（1）Described high-level oxidation technology can be ozone oxidation, iron-carbon micro-electrolysis, Fenton oxidation etc., while part COD is removed, can improve the biodegradability of waste water.It is preferred that using ozone oxidation, Lincomycin wastewater is sent into ozone-oxidizing device, by ozone oxidation agent, by the difficult for biological degradation oxidation operation in waste water into the lower-molecular substance of biodegradability, reach improvement wastewater biodegradability purpose.Described ozone-oxidizing device use reaction tower or contact-oxidation pool, ozone oxidation agent by processing unit outside ozone generator provide.The dosage of ozone oxidation agent is 20-50mg/L waste water, preferably 30-40mg/L waste water；Ozone oxidation agent gas access concentration is 50-120mg/L, preferably 100-120mg/L；Hydraulic detention time of the waste water in reaction tower or oxidation pond is 10-60min, preferably 20-30min.By the processing unit, water outlet BOD5/COD≥0.4。
Step of the present invention（2）Described coagulating sedimentation can typically be used using conventional coagulation sedimentation method to dosing coagulant, flocculant, flocculation aid or triplicity in waste water.Described coagulant can be the low-molecular inorganic materials such as alum, aluminum sulfate, chloro sulphuric acid aluminium, ferric sulfate, ferrous sulfate, iron chloride and ferric trichloride, or the inorganic polymer material such as aluminium polychloride, polyaluminium sulfate, polychloride ferro-aluminum, bodied ferric sulfate；Described flocculant can be polyacrylamide, polypropylene acid polymer；Described flocculation aid can be lime, ozone, hydrogen peroxide, potassium permanganate etc..It is preferred that by the way of organic flocculant and inorganic flocculating agent combination is added, wherein organic flocculant is polyacrylamide, and dosage is 3-10mg/L；Inorganic flocculating agent uses bodied ferric sulfate or ferric trichloride, and dosage is 20-100mg/L.Purpose is to remove the suspension in waste water, and adjusts the pH value of waste water, to mitigate the processing load of subsequent biochemical unit.Dosage determines that suspension is reduced within 1000mg/L after treatment according to concentration of suspension.
In the present invention, step（2）Filtering can use various filter plants well known to those skilled in the art.Can be such as separated by filtration using batch centrifuge, rotating speed is controlled in 1000-5000rpm, and centrifugation time is 5-10min.
In the present invention, step（3）Described anaerobic biochemical treatment method can be anaerobic baffled reactor（ABR）, anaerobic fluidized bed, up flow anaerobic sludge blanket reactor（UASB）, internal-circulation anaerobic reactor（IC）, be thoroughly mixed in formula anaerobic reation pool, expanded granular sludge bed reactor reactor (EGSB) etc. any one.Anaerobic biochemical treatment condition is：Hydraulic detention time is controlled for 6-48h, pH is 6.0-9.0, temperature is 20-55 DEG C.Purpose is mainly and for macromolecular substances to be degraded to the biochemical material of small molecule, reduces follow-up Aerobic Process for Treatment load.
In the present invention, step（4）Described biological contact oxidation turns to the reaction tank of conventional treatment waste water, such as sequence batch activated sludge process（SBR）, loop cycle activated Sludge System（CASS）, membrane bioreactor（MBR）, BAF（BFT）And moving bed membrane bioreactor（MBBR）Any one in.Treatment conditions are：Hydraulic detention time is 6-48h, and pH is 6.0-9.0, and dissolved oxygen concentration is 1-5mg/L, and temperature is 20-37 DEG C.The purpose of bio-contact oxidation is further to remove COD, the COD in waste water is met emission request.
In the present invention, step（4）Described secondary coccus（Paracoccus sp.）FSTB-2 bacterial strains and Pseudomonas stutzeri（Pseudomonas stutzeri）FSTB-5, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC）；Preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica；Deposit number is respectively：CGMCC No.10938、CGMCCNo.10940；Preservation date：On June 1st, 2015.The main morphological features of secondary coccus FSTB-2 are：Colony colour is ecru, and it is spherical that bacterial strain is individual；Physiological and biochemical property is：Gram's staining is feminine gender, and oxidase positive, catalase is negative, and decomposable asymmetric choice net utilizes several kinds of carbon source, with nitrate reduction activity；It is resistant to one or more in lincomycin, lincomycin S V, acidum nalidixicum, guanidine hydrochloride etc..Pseudomonas stutzeri（Pseudomonas stutzeri）The main morphological features of FSTB-5 are：Colony colour is shallow ginger color, and it is shaft-like that bacterial strain is individual；Physiological and biochemical property shows as：Gram-negative, oxidase negative contacts enzyme positive, and with nitrate reduction performance, decomposable asymmetric choice net utilizes several kinds of carbon source；It is resistant to one or more in lincomycin, minocycline, lincomycin S V, troleandomycin, vancomycin, AZT, acidum nalidixicum etc..
In the present invention, the secondary coccus FSTB-2 and Pseudomonas stutzeri FSTB-5 for being added can be added using the dense bacterium solution for directly preparing, it is also possible to add nutriment, preservation auxiliary agent etc. to be prepared into microbial inoculum in dense bacterium solution standby.The specific preparation method of described dense bacterium solution is：
（1）Secondary coccus FSTB-2, Pseudomonas stutzeri FSTB-5 are inoculated into the inclined-plane of FSTB solid mediums or flat board respectively, 25-40 DEG C is cultivated 24-48 hours；
（2）Liquid seeds liquid culture：FSTB fluid nutrient mediums are prepared, is sub-packed in triangular flask, after sterilizing and being cooled to room temperature, in the inoculation activated in picking inclined-plane or flat board under gnotobasis to triangular flask, 25-40 DEG C is cultivated 24-72 hours.The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value is 6.0-8.5, preferably 6.5-8.0；FSTB solid mediums are the agar of addition 20g/L in liquid medium within；
（3）Aeration culture：FSTB fluid nutrient mediums are added in the reactor for be provided with aerator, ratio inoculation liquid seed liquor according to reactor volume than 5%-25%, pH value is controlled in 6.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, also a small amount of carbon source can be added, nitrogen source and microelement substance, culture 24-48 hours is 1 cultivation cycle, the nutrient solution of corresponding volume is discharged according to aforementioned proportion afterwards, thus the dense bacterium solution containing high concentration thalline is obtained.
In the present invention, the dense bacterium solution product that above-mentioned culture is obtained is added in wastewater treatment system according to the 0.01%-1% of handled wastewater volume per hour, is preferably added according to 0.1%-0.5%.
Compared with prior art, the invention has the advantages that：
（1）The present invention is using advanced oxidation-coagulating sedimentation-anaerobic biological-contact oxidation technology treatment Lincomycin wastewater, and add the de- COD bacterium of specific salt tolerant in catalytic oxidation unit, it is resistant to the antibiotic in waste water, the COD efficient removals in waste water are realized, with process is simple, treatment effeciency be high, the low feature of processing cost.
（2）Secondary coccus FSTB-2 and Pseudomonas stutzeri FSTB-5 can be applied not only to the efficient removal of COD in brine waste, the antibiotic in waste water can particularly be tolerated, the biochemical treatment of Lincomycin wastewater is especially suitable for, processing cost low advantage small with dosage.
Specific embodiment
Technical scheme and effect are described in detail with reference to embodiment, but are not so limited the present invention.
The secondary coccus FSTB-2 and the cultural method of Pseudomonas stutzeri FSTB-5 that the embodiment of the present invention is used include bacterial strain activation, the culture of liquid seeds liquid, aeration culture, and detailed process is as follows：
（1）Bacterial strain is activated：Secondary coccus FSTB-2 and Pseudomonas stutzeri FSTB-5 are inoculated on FSTB solid mediums respectively and are activated, cultivated 48 hours under 35 DEG C of environment, be then stored in stand-by in 4 DEG C of refrigerators.FSTB solid mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, agar 20g/L, pH value 7.8；
（2）Liquid seeds liquid culture：FSTB fluid nutrient mediums are prepared, is sub-packed in triangular flask, after sterilizing and being cooled to room temperature, with the inoculation after being activated in oese picking flat board to triangular flask under gnotobasis, cultivated 48 hours under the conditions of 35 DEG C.The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value 7.8；
（3）Aeration culture：Cultivated using closed reactor,It is complete that institute is required to sterilizing using reactor and various utensils,Air inlet and exhaust position need to install bacteriological filter apparatus,Nutrient solution,Acid-base modifier and trace element solution are added after being required to sterilizing according to sterile working code,Reactor need to be equipped with aerator,And can carry out into water,Acid adjustment,Alkali tune,Feed supplement and draining discharge are operated,The FSTB fluid nutrient mediums after sterilizing are put into the reactor,Ratio according to percent by volume 10% is inoculated with liquid seed liquor,After opening incubation,Use soda acid automatic control system by medium pH value scope control in 6.0-8.5 in incubation,Aeration culture carries out periodic feed supplement and discharge operation for 72 hours afterwards,Discharge is the nutrient solution of the 25% of reactor volume,Feed supplement is the FSTB fluid nutrient mediums of reactor volume 25%,Culture 24 hours is 1 cultivation cycle,The nutrient solution of corresponding volume is discharged according to aforementioned proportion afterwards,Thus the dense bacterium solution product containing corresponding pure bacterial strain is obtained.
Embodiment 1
The waste water quality that certain factory lincomycin production technology is produced is：COD is 8000mg/L, and salt content is 1.5wt%, and SS is 2000mg/L, and ammonia nitrogen concentration 20mg/L, pH are 8.0.
Lincomycin wastewater is processed using Ozonation first, the dosage of ozone oxidation agent is 30mg/L, and ozone oxidation agent gas access concentration is 100mg/L, and hydraulic detention time is 30min, ozone oxidation water outlet BOD5/COD≥0.6.Then coagulating kinetics are carried out, polyacrylamide is added first, polyacrylamide dosage is 5mg/L, then adds bodied ferric sulfate, and dosage is 60mg/L.Concentration of suspension is down to below 500mg/L in coagulation water outlet.Removal precipitation is filtered after coagulating sedimentation, is filtered using batch centrifuge, rotating speed is controlled in 3000rpm, and centrifugation time is 5min.Water outlet enters internal-circulation anaerobic reactor（IC）, service condition is：Hydraulic detention time is 24h, and pH value is 8.0-8.5, and temperature is 35 DEG C.Inner circulation reactor water outlet enters biological contact oxidation pond, and service condition is：Hydraulic detention time is 12 hours, and pH value is 8.0-8.5, and dissolved oxygen concentration is 2-3mg/L, and temperature is 30 DEG C.By after above-mentioned treatment, effluent COD concentration is less than 200mg/L, ammonia nitrogen concentration is less than 15mg/L.
Embodiment 2
The waste water quality that certain factory lincomycin production technology is produced is：COD is 11000mg/L, and salt content is 2wt%, and SS is 3000mg/L, and ammonia nitrogen concentration 50mg/L, pH are 8.5.
Lincomycin wastewater is processed using Ozonation first, the dosage of ozone oxidation agent is 40mg/L, and ozone oxidation agent gas access concentration is 120mg/L, and hydraulic detention time is 40min, ozone oxidation water outlet BOD5/COD≥0.6.Then coagulating kinetics are carried out, polyacrylamide is added first, polyacrylamide dosage is 7mg/L, then adds ferric trichloride, and dosage is 50mg/L.Concentration of suspension is down to below 500mg/L in coagulation water outlet.Removal precipitation is filtered after coagulating sedimentation, is filtered using batch centrifuge, rotating speed is controlled in 3000rpm, and centrifugation time is 5min.Water outlet enters up flow anaerobic sludge blanket reactor（UASB）, service condition is：Hydraulic detention time is 24h, and pH value is 8.5-9.0, and temperature is 35 DEG C.Up flow anaerobic sludge blanket reactor water outlet enters biological contact oxidation pond, and service condition is：Hydraulic detention time is 24 hours, and pH value is 8.5-9.0, and dissolved oxygen concentration is 2-3mg/L, and temperature is 30 DEG C.By after above-mentioned treatment, effluent COD concentration is less than 200mg/L, ammonia nitrogen concentration is less than 25mg/L.
Embodiment 3
, with embodiment 1, difference is for handling process and operating condition：In step（4）Aerobic treatment unit adds the secondary dense bacterium solutions of coccus FSTB-2 according to the 0.3% of handled wastewater volume per hour, and COD concentration is less than 100mg/L in final outflow water after treatment, and ammonia nitrogen concentration stabilization is less than 10mg/L.
Embodiment 4
, with embodiment 2, difference is for handling process and operating condition：In step（4）Aerobic treatment unit adds the dense bacterium solutions of Pseudomonas stutzeri FSTB-5 according to the 0.5% of handled wastewater volume per hour, and COD concentration is less than 100mg/L in final outflow water after treatment, and ammonia nitrogen concentration stabilization is less than 20mg/L.
Embodiment 5
, with embodiment 2, difference is for handling process and operating condition：In step（4）According to the 0.5% of the handled wastewater volume per hour mixed bacteria liquid for adding secondary coccus FSTB-2 and Pseudomonas stutzeri FSTB-5, bacterium solution volume ratio is 1 to aerobic treatment unit:1, COD concentration is less than 80mg/L in final outflow water after treatment, and ammonia nitrogen concentration stabilization is less than 20mg/L.
Claims (13)
1. a kind of rifamycin produces the processing method of waste water, it is characterised in that comprise the following steps：
（1）Advanced oxidation treatment：Lincomycin wastewater is pre-processed using high-level oxidation technology；
（2）Coagulating kinetics：Coagulating sedimentation, the precipitation that filtering removal is produced are carried out to oxidation water outlet；
（3）Anaerobic biochemical treatment：To step（2）Water outlet carries out anaerobic biochemical treatment after filtering；
（4）Biological reinforced treatment：Anaerobic effluent is processed using biological contact oxidation process, while adding the de- COD bacterium of salt tolerant, the de- COD bacterium of the salt tolerant are secondary coccus（Paracoccus sp.）FSTB-2 and/or Pseudomonas stutzeri（Pseudomonas stutzeri）FSTB-5, is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, and deposit number is CGMCCNo.10938, CGMCCNo.10940.
2. method according to claim 1, it is characterised in that：The Lincomycin wastewater is mainly derived from technique waste water and washes during lincomycin production of raw medicine, and waste water quality is：COD（Chromium method, similarly hereinafter）It is 6000-18000mg/L, salt content is 0.1wt%-2.5wt%, and suspension is 500-5000mg/L, and ammonia nitrogen concentration is 5-200mg/L, pH value 7.5-10.
3. method according to claim 1, it is characterised in that：Step（1）Described high-level oxidation technology is ozone oxidation, iron-carbon micro-electrolysis or Fenton oxidation.
4. the method according to claim 1 or 3, it is characterised in that：Step（1）Described advanced oxidation uses ozone oxidation, and the ozone-oxidizing device uses reaction tower or contact-oxidation pool, ozone oxidation agent by processing unit outside ozone generator provide；The dosage of ozone oxidation agent is 20-50mg/L waste water, and ozone oxidation agent gas access concentration is 50-120mg/L, and hydraulic detention time is 10-60min.
5. method according to claim 1, it is characterised in that：Step（2）Described coagulating sedimentation is used to dosing coagulant, flocculant, flocculation aid or triplicity in waste water；Described coagulant is alum, aluminum sulfate, chloro sulphuric acid aluminium, ferric sulfate, ferrous sulfate, iron chloride and ferric trichloride low-molecular inorganic material, or is aluminium polychloride, polyaluminium sulfate, polychloride ferro-aluminum, bodied ferric sulfate inorganic polymer material；Described flocculant is polyacrylamide, polypropylene acid polymer；Described flocculation aid is lime, ozone, hydrogen peroxide or potassium permanganate.
6. method according to claim 1 or 5, it is characterised in that：Step（2）By the way of organic flocculant and inorganic flocculating agent combination is added, wherein organic flocculant is polyacrylamide to described coagulating sedimentation, and dosage is 3-10mg/L；Inorganic flocculating agent uses bodied ferric sulfate or ferric trichloride, and dosage is 20-100mg/L.
7. method according to claim 1, it is characterised in that：Step（3）Described anaerobic biochemical treatment method be anaerobic baffled reactor, anaerobic fluidized bed, up flow anaerobic sludge blanket reactor, internal-circulation anaerobic reactor, be thoroughly mixed in formula anaerobic reation pool, expanded granular sludge bed reactor reactor any one；Treatment conditions are：Hydraulic detention time is 6-48h, and pH is 6.0-9.0, and temperature is 20-55 DEG C.
8. method according to claim 1, it is characterised in that：Step（4）Described biological contact oxidation process is any one in sequence batch activated sludge process, loop cycle activated Sludge System, membrane bioreactor, BAF, moving bed membrane bioreactor；Treatment conditions are：Hydraulic detention time is 6-48h, and pH is 6.0-9.0, and dissolved oxygen concentration is 1-5mg/L, and temperature is 20-37 DEG C.
9. method according to claim 1, it is characterised in that：Step（4）The main morphological features of described secondary coccus FSTB-2 are：Colony colour is ecru, and it is spherical that bacterial strain is individual；Physiological and biochemical property is：Gram's staining is feminine gender, and oxidase positive, catalase is negative, and decomposable asymmetric choice net utilizes several kinds of carbon source, with nitrate reduction activity；It is resistant to one or more in lincomycin, lincomycin S V, acidum nalidixicum, guanidine hydrochloride etc.；Pseudomonas stutzeri（Pseudomonas stutzeri）The main morphological features of FSTB-5 are：Colony colour is shallow ginger color, and it is shaft-like that bacterial strain is individual；Physiological and biochemical property shows as：Gram-negative, oxidase negative contacts enzyme positive, and with nitrate reduction performance, decomposable asymmetric choice net utilizes several kinds of carbon source；It is resistant to one or more in lincomycin, minocycline, lincomycin S V, troleandomycin, vancomycin, AZT, acidum nalidixicum etc..
10. method according to claim 1, it is characterised in that：Step（4）The secondary coccus FSTB-2 and Pseudomonas stutzeri FSTB-5 that are added are added using the dense bacterium solution for directly preparing, or nutriment is added in dense bacterium solution, that preservation auxiliary agent is prepared into microbial inoculum is standby.
11. methods according to claim 10, it is characterised in that：Step（4）The specific preparation method of the secondary coccus FSTB-2 and the dense bacterium solutions of Pseudomonas stutzeri FSTB-5 is：
（1）Secondary coccus FSTB-2, Pseudomonas stutzeri FSTB-5 are inoculated into the inclined-plane of FSTB solid mediums or flat board respectively, 25-40 DEG C is cultivated 24-48 hours；
（2）Liquid seeds liquid culture：FSTB fluid nutrient mediums are prepared, is sub-packed in triangular flask, after sterilizing and being cooled to room temperature, in the inoculation activated in picking inclined-plane or flat board under gnotobasis to triangular flask, 25-40 DEG C is cultivated 24-72 hours；
（3）Aeration culture：FSTB fluid nutrient mediums are added in the reactor for be provided with aerator, ratio inoculation liquid seed liquor according to reactor volume than 5%-25%, pH value is controlled in 6.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, culture 24-48 hours is 1 cultivation cycle, the nutrient solution of corresponding volume is discharged according to aforementioned proportion afterwards, the dense bacterium solution containing high concentration thalline is thus obtained.
12. methods according to claim 11, it is characterised in that：The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value is 6.0-8.5, preferably 6.5-8.0；FSTB solid mediums are addition 20g/L agar in liquid medium within.
13. method according to claim 10 or 11, it is characterised in that：The dense bacterium solution product for obtaining will be cultivated to be added in wastewater treatment system according to the 0.01%-1% of handled wastewater volume per hour.
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