CN114620841B - Microbial pretreatment method for rifamycin-containing wastewater - Google Patents
Microbial pretreatment method for rifamycin-containing wastewater Download PDFInfo
- Publication number
- CN114620841B CN114620841B CN202210139514.XA CN202210139514A CN114620841B CN 114620841 B CN114620841 B CN 114620841B CN 202210139514 A CN202210139514 A CN 202210139514A CN 114620841 B CN114620841 B CN 114620841B
- Authority
- CN
- China
- Prior art keywords
- wastewater
- biochemical
- genus
- biochemical treatment
- rifamycin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/343—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the pharmaceutical industry, e.g. containing antibiotics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to the field of water treatment, and discloses a microbial pretreatment method for rifamycin-containing wastewater, which comprises the following steps: the method comprises the following steps: selecting a mixed strain as an initial biochemical microbial inoculum; step two: adding the mixed strains into a wastewater biochemical system for bacterial biochemical treatment; step three: adding growth factors to the wastewater; step four: taking a bacterial liquid after a wastewater biochemical system is stable, and determining the final genus proportion and dominant genus; step five: in the operation process, when the treatment effect is not good, the ratio of each genus in the inoculated mixed strain is adjusted to be consistent with the ratio of the genus in the step four according to the genus ratio result in the step four. The invention can degrade the nonbiodegradable biotoxic substances in the wastewater into nontoxic substances through screening out mixed strains with stronger resistance to the rifamycin and bacterial biochemical treatment, quickly and economically convert the rifamycin-containing wastewater which cannot be directly treated by microorganisms into low-concentration wastewater, and greatly improve the biochemical property.
Description
Technical Field
The invention relates to the field of water treatment, in particular to a microbial pretreatment method for rifamycin-containing wastewater.
Background
Antibiotics are a great discovery in human history, have been widely used in a plurality of fields such as medical treatment and the like, and effectively ensure the health of human bodies. However, in the process of antibiotic production and application, a large amount of refractory organic wastewater containing antibiotics can be generated, and if the refractory organic wastewater is directly discharged, the water environment can be seriously harmed.
Rifamycin antibiotics are antibiotics produced by Streptomyces mediterranei, have molecular structures similar to phosphoenolpyruvate, can compete with bacteria for the same transferase, inhibit the synthesis of bacterial cell walls to cause bacterial death, and have broad-spectrum antibacterial action, thereby having inhibitory action on microorganisms of biochemical treatment process.
The rifamycin production wastewater mainly comprises production process wastewater, washing wastewater, cooling sewage and the like. The wastewater has complex components and high concentration of organic matters, soluble or colloidal solids, contains biological toxic substances such as antibiotics with difficult biodegradation and bacteriostasis effects, and belongs to high-concentration organic wastewater containing the biological toxic substances with difficult biodegradation.
As the rifamycin wastewater is characterized by high toxicity and poor biodegradability, the rifamycin wastewater is generally pretreated to reduce the biotoxicity and improve the biodegradability before entering a conventional activated sludge system for treatment. At present, there are many common wastewater pretreatment means, such as iron-carbon microelectrolysis, fenton oxidation, wet oxidation, electrocatalysis and other physical and chemical means, and although suitable physical and chemical methods are immediately available, the equipment investment is high, the energy consumption is high, and secondary pollution is easily caused. Therefore, it is necessary to develop a new, more efficient and low-cost treatment method for high-concentration and high-toxicity antibiotic wastewater.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for pretreating rifamycin-containing wastewater by using microorganisms. Aiming at the waste water containing rifamycin. The mixed strain CHMS with strong adaptability and obvious effect is screened from the natural environment, has strong resistance to rifamycin, can degrade difficultly-degradable biological toxic substances in the wastewater into non-toxic substances through bacterial biochemical treatment of the mixed strain, quickly and economically converts rifamycin-containing wastewater which cannot be directly treated by microorganisms into low-concentration wastewater, greatly improves the biochemical property, removes 60-80% of COD and ammonia nitrogen in the wastewater, and reduces the treatment burden of a subsequent conventional biochemical system by about 60-80%. There is no report in the prior art that the above-mentioned genera can be used for the microbial pretreatment of rifamycin-containing wastewater.
The specific technical scheme of the invention is as follows: a method for pretreating rifamycin-containing wastewater by microorganisms comprises the following steps:
the method comprises the following steps: selecting a mixed strain CHMS as an initial biochemical inoculant; the mixed strain comprises: 30-33% of Dysgonomonas, 29-32% of Brevundimonas, 8-12% of Sphingobacterium, 5-9% of Erysipelothrix Erysipelothrix, and the balance of other genera.
Step two: adding the mixed strain into a wastewater biochemical system containing rifamycin wastewater to carry out bacterial biochemical treatment;
step three: growth factors required for growth of dominant bacteria are added into the wastewater to optimize the pretreatment effect.
Step four: after the wastewater biochemical system is stabilized, taking bacterial liquid, extracting microbial genome DNA, performing high-throughput sequencing on microbial structures in the wastewater biochemical system, and determining the final genus proportion and dominant genus.
Step five: in the operation process, when the treatment effect is not good, the ratio of each genus in the inoculated mixed strain is adjusted to be consistent with the ratio of the genus in the step four according to the genus ratio result in the step four.
In earlier researches, the invention discovers that the antibiotic wastewater has complex components, contains carbohydrates, proteins, waste mycelia, organic solvents, high concentration of fermentation residual nutrients, high concentration of suspended matters, high chromaticity, fermentation liquor and ester odor, and contains a large amount of biological toxic substances, such as residual antibiotics and metabolites thereof, surfactants, acids, alkalis, DMF, esters and other organic and inorganic compounds. However, the organic substances of such wastewater are biochemical for microorganisms, and organic solvents such as Dimethylformamide (DMF) and Tetrahydrofuran (THF) in wastewater are major contributors to the biological decomposition and utilization, and by selecting an appropriate mixed strain, it is possible to achieve biological pretreatment of high-concentration wastewater and further detoxification treatment.
Aiming at the waste water containing rifamycin, the mixed strain CHMS with strong adaptability and obvious effect is screened from the natural environment, the mixed strain has stronger resistance to rifamycin, the difficultly degradable biological toxic substances in the waste water can be degraded into non-toxic substances through the bacterial biochemical treatment of the CHMS mixed strain, the rifamycin-containing waste water which cannot be directly treated by microorganisms is quickly and economically converted into low-concentration waste water, the biochemical property is greatly improved, 60-80% of COD (chemical oxygen demand) and ammonia nitrogen in the waste water are removed, and the treatment load of a subsequent conventional biochemical system is reduced by about 60-80%. There is no report in the prior art that the above-mentioned genera can be used for the microbial pretreatment of rifamycin-containing wastewater.
The mixed strain CHMS has the following characteristics:
(1) Under the culture condition containing rifamycin (0.1% -2%) with different concentrations, the relative abundance of various bacteria is basically stable;
(2) Higher rifamycin degrading activity;
(3) The mixed strain is preserved at-80 ℃ and prepared into a freezing tube containing 20% of glycerol, and an LB culture medium is used during recovery;
(4) The culture conditions of the mixed strains are as follows: temperature 20-35 ℃ (25 ℃ optimum), pH =6.0-8.5 (pH =7.0 optimum), salinity 0.5w/v% -4.5w/v% (1.5 w/v% optimum).
In the mixed system, four different main bacteria can exert enzyme production characteristics of the bacteria, and play roles of complementing and mutually promoting through enzyme system complementation, so that the bacteria are widely coexisted all the time in the nature, and certain ecological symbiosis often exists between the bacteria and the bacteria. In the degradation process of complex compounds, one microorganism cannot be independently completed or can only be weakly performed, and two or more microorganisms are required to be jointly completed, so that the mutual survival among bacteria is realized by independently or synergistically degrading different substances through respective metabolic activities.
The rifamycin wastewater is complex in components and contains organic and inorganic compounds such as carbohydrates, proteins, waste mycelia, organic solvents, residual antibiotics and metabolites thereof, surfactants, acids, alkalis, DMF, THF, esters and the like. Organic solvents such as Dimethylformamide (DMF) and Tetrahydrofuran (THF) and other organic compounds in the wastewater are beneficial to the biochemistry of the four strains, and the four strains belong to hydrolytic acid-producing bacteria and can convert complex organic matters into simple organic matters. The hydrolysis mainly takes place extracellularly, and the end product of biochemical treatment under the action of extracellular enzyme is volatile fatty acid for exercise. Rifamycin degradation in wastewater is catalyzed primarily by enzymes produced by Dysgonomonas and Brevundimonas, which catalyze the ring-opening inactivation of rifamycin by enzymes.
The reason why the ratio of the four types of bacteria in the mixed bacteria species is within the above range in the present invention is that: dysgonomonas and Brevundimonas are contributors for degrading rifamycin in wastewater, belong to dominant strains in wastewater, and therefore the ratio of the Dysgonomonas to Brevundimonas in a compound strain is large; sphingobacterium and Erysipelothrix are primarily involved in degrading other constituents in wastewater.
Preferably, the other strains include 4-8% of Pseudomonas pseudochromyomatis, 2-6% of Pseudomonas pseudomonads, 3-5% of Vagococcus roaming, and 2-4% of Paracoccus Paracoccus.
Preferably, the microorganism pretreatment method is suitable for rifamycin-containing wastewater meeting the following indexes: the waste water is highly toxic, the proportion of biochemical organic carbon in the waste water to the total organic carbon is more than 50 percent, the ratio of BOD5/CODcr is 0.4 +/-0.1, and the CODcr is 10000-20000 mg/L.
Preferably, the second step specifically comprises: transferring the wastewater to a water quality adjusting tank, leading the wastewater to a biochemical treatment tank after water quality adjustment, and adding urea, phosphate and 15-25w/v% of mixed strains into the biochemical treatment tank; the early COD =5000-10000mg/L low-load intermittent water inlet, the DO is controlled at 2-5mg/L and the temperature is controlled at 25-30 ℃; after the concentration of the thalli in the biochemical treatment tank is stable and the removal rate of the thalli to the wastewater CODcr reaches more than 40%, carrying out high-load continuous water inflow with COD =10000-20000mg/L, and entering an operation control stage; in this stage, CODcr and NH3-N indexes of inlet water and outlet water of biochemical treatment tank are monitored every day to detect biochemical treatment effect until the CODcr removing rate of waste water is above 60%.
The reason that the water is fed intermittently under low load and is fed continuously under high load at the later stage is as follows: the proportion of each bacterium in the initially added mixed bacterium CHMS is approximate proportion, although the mixed bacterium CHMS has universality, the mixed bacterium CHMS is not the optimal proportion for specific wastewater, so the effect of the initial low-load intermittent water feeding is to culture the bacterium, more bacterium is gradually adapted to the wastewater, and the domestication effect is achieved; and continuously feeding water after the water is stabilized.
Preferably, in the second step, urea and phosphorus salt are added to ensure that the molar ratio of C to N to P in the wastewater is 200:5:1.
Preferably, in the second step, an aeration system is arranged at the bottom of the biochemical treatment tank, and wastewater enters the bottom of the biochemical treatment tank and exits from the upper part of the biochemical treatment tank; the side wall of the biochemical treatment tank is provided with a sludge discharge port, the effluent of the biochemical treatment tank is connected with an MBR membrane treatment module for MBR membrane treatment, and suspended bacteria intercepted by the MBR membrane flow back to the biochemical treatment tank.
The lower inlet and the upper outlet can play a good role in mixing flow, the thalli can reach a suspension state in the system when the upward impact action of bottom water inlet and aeration and the downward sedimentation action of the thalli reach balance, and the treatment effect is optimal.
Preferably, in the second step, the MBR membrane is a polyvinylidene fluoride hollow fiber membrane with a membrane pore size of 0.2 microns.
Preferably, in step three, the growth factors are vitamins, trace elements and biotin.
Preferably, in step four, the dominant genera are the top-ranking several genera, and the sum of the abundances of the dominant genera exceeds 50%.
The abundance was set to over 50% because it can be regarded as the species that plays a major role.
Preferably, in the fifth step, the flag of poor processing efficiency is: the removal rate of CODcr of the wastewater is lower than 40 percent, or the concentration of the bacteria in the bacteria liquid is lower than 50 percent of that of the bacteria when the normal treatment efficiency is achieved.
Compared with the prior art, the invention has the beneficial effects that: the mixed strain CHMS with strong adaptability and obvious effect is screened from the natural environment, has strong resistance to rifamycin, can degrade difficultly-degradable biological toxic substances in the wastewater into non-toxic substances through bacterial biochemical treatment of the mixed strain, quickly and economically converts rifamycin-containing wastewater which cannot be directly treated by microorganisms into low-concentration wastewater, greatly improves the biochemical property, removes 60-80% of COD and ammonia nitrogen in the wastewater, and reduces the treatment burden of a subsequent conventional biochemical system by about 60-80%. There is no report in the prior art that the above-mentioned genera can be used for the microbial pretreatment of rifamycin-containing wastewater.
Drawings
FIG. 1 is a graph showing the change tendency of COD in nearly one week before and after the treatment of wastewater in example 1;
FIG. 2 is a graph showing the change of NH3-N at about one week before and after the treatment of wastewater in example 1.
Detailed Description
The present invention will be further described with reference to the following examples.
General examples
A method for pretreating rifamycin-containing wastewater by microorganisms is suitable for rifamycin-containing wastewater meeting the following indexes: the wastewater is highly toxic, the proportion of biochemical organic carbon in the wastewater in the total organic carbon is more than 50 percent, the ratio of BOD5/CODcr is 0.4 +/-0.1, and the CODcr is 10000-20000 mg/L.
The method comprises the following steps:
the method comprises the following steps: selecting a mixed strain CHMS as an initial biochemical inoculant; the mixed strain comprises: 30-33% of Dysgonomonas, 29-32% of Brevundimonas, 8-12% of Sphingobacterium, 5-9% of Erysipelothrix erysipelomyces, 4-8% of Pseudomonas pseudochromyelia, 2-6% of Pseudomonas, 3-5% of Vagococcus roaming and 2-4% of Paracoccus.
Step two: adding the mixed strain into a wastewater biochemical system containing the rifamycin wastewater to carry out bacterial biochemical treatment. The method specifically comprises the following steps: transferring the wastewater to a water quality adjusting tank, leading the wastewater to a biochemical treatment tank after water quality adjustment, and adding urea, phosphate and 15-25w/v% of mixed strains into the biochemical treatment tank; wherein, urea and phosphate are added to ensure that the molar ratio of C to N to P in the wastewater is 200 to 5 to 1. The early COD =5000-10000mg/L low-load intermittent water inlet, the DO is controlled at 2-5mg/L and the temperature is controlled at 25-30 ℃; after the concentration of thalli in the biochemical treatment tank is stable and the removal rate of the thalli to the CODcr of the wastewater reaches more than 40%, carrying out high-load continuous water inflow with COD =10000-20000mg/L, and entering an operation control stage; in this stage, CODcr and NH3-N indexes of inlet water and outlet water of biochemical treatment tank are monitored every day to detect biochemical treatment effect until the CODcr removing rate of waste water is above 60%. Wherein, the bottom of the biochemical treatment tank is provided with an aeration system, and wastewater enters the bottom of the biochemical treatment tank and exits from the upper part; the side wall of the biochemical treatment tank is provided with a sludge discharge port, the effluent of the biochemical treatment tank is connected with an MBR (membrane-bioreactor) membrane (polyvinylidene fluoride hollow fiber membrane with a membrane aperture of 0.2 micron) treatment module for MBR membrane treatment, and suspended bacteria intercepted by the MBR membrane flow back to the biochemical treatment tank.
Step three: growth factors (vitamins, trace elements and biotin) required for growth of the dominant bacteria are added into the wastewater to optimize the pretreatment effect.
Step four: after the wastewater biochemical system is stabilized, taking a bacterial solution, extracting microbial genome DNA, performing high-throughput sequencing on microbial structures in the wastewater biochemical system, and determining the final genus proportion and dominant genera (a plurality of genera with the most top abundance ranking, and the sum of the abundances of the dominant genera exceeds 50%).
Step five: during the operation, when the treatment effect is not good (the mark is that the removal rate of CODcr of the wastewater is lower than 40 percent, or the concentration of the bacteria in the bacteria liquid is lower than 50 percent of the concentration of the bacteria when the normal treatment efficiency is achieved), the proportion of each bacteria in the inoculated mixed bacteria is adjusted to be consistent with the ratio of the bacteria in the step four according to the result of the ratio of the bacteria in the step four.
And (3) performing a comparison test on the compounding effect of different strains:
in order to prove that four bacteria in the mixed bacteria can generate synergistic effect, the following experiments are carried out: CHMS with different compound combinations is inoculated in a rifamycin-containing culture medium with the concentration of 2%, after 2 days of culture under proper conditions, the rifamycin concentration is determined by an HPLC gradient elution method, and the optimal combination of degradation effects is obtained. The rifamycin degradation rates under different compound combinations are shown as follows
CHMS-0: dysgonomonas (33%), brevundimonas (31%), sphingobacterium (10%), erysipelothrix (7%), pseudochromycotacterium pseudochromycotacterium (6%), pseudomonas (5%), vagococcus (5%), paracoccus (3%);
CHMS-1: brevundimonas (31%), sphingobacterium (10%), erysipelothrix (7%), pseudochrobacterium pseudochromycota (6%), pseudomonas (5%), vagococcus nomococcus (5%), paracoccus (3%);
CHMS-2: dysgonomonas (33%), sphingobacterium (10%), erysipelothrix (7%), pseudochrobacter pseudochromycota (6%), pseudomonas (5%), vagococcus nomococcus (5%), paracoccus (3%);
CHMS-3: dysgonomonas (33%), brevundimonas (31%), erysipelothrix (7%), pseudochromyrobacterium pseudochromyelium (6%), pseudomonas (5%), vagococcus nomococcus (5%), paracoccus (3%);
CHMS-4: dysgonomonas (33%), brevundimonas (31%), sphingobacterium (10%), pseudomonas pseudochrom (6%), pseudomonas pseudomonads (5%), vagococcus nomus (5%), paracoccus (3%)
The degradation rate of a normal compound strain CHMS-0 is 98.79 percent, and the degradation rates of rifamycins for treating CHMS-1, CHMS-2, CHMS-3 and CHMS-4 by respectively removing one strain (the proportion of other key strains is unchanged) are respectively as follows: 70.27%, 65.57%, 88.76% and 85.67%, and the degradation rate is obviously reduced by 28.52%, 33.22%, 10.03% and 13.12%.
Resistance test of mixed strains to rifamycin:
for mixed strains CHMS-0 (Dysgonomonas (33%), brevundimonas (31%), sphingobacterium (10%), erysipelothrix (7%)),The rifamycin resistance test was performed on pseudochrobacterum (6%), pseudomonas (5%), vacoccus (5%), paracoccus (3%)), as follows: preparing LB liquid culture medium containing rifamycins with different concentrations, wherein the rifamycins are respectively as follows: 0%, 0.1%, 0.25%, 0.5%, 0.75%, 1%, 1.25%, 1.5%, 1.75%, 2%, 2.5%, 3%, 3.5%, 4%, 5%, 10%, measuring OD after inoculating CHMS-0 into the medium and culturing under appropriate conditions for 2 days 600 Values, data are as follows:
| rifamycin% | 0.00％ | 0.10％ | 0.25％ | 0.50％ | 0.75％ | 1％ | 1.25％ | 1.50％ |
| OD 600 | 1.356 | 1.247 | 1.313 | 1.256 | 1.342 | 1.259 | 1.253 | 1.278 |
| Rifamycin% | 1.75％ | 2％ | 2.50％ | 3％ | 3.5％％ | 4％ | 5％ | 10％ |
| OD 600 | 1.186 | 1.157 | 0.345 | 0.241 | 0.112 | 0.057 | 0.023 | 0.018 |
As can be seen from the above table, CHMS is considered to have a superior effect of degrading rifamycin at a rifamycin concentration of 0 to 2%.
Example 1: an engineering example of biological treatment of wastewater containing rifamycin in a certain enterprise in Zhejiang.
Analyzing the water quality of the wastewater: high-concentration wastewater contains residual antibiotics, microbial metabolites, surfactants, acids, alkalis, dimethyl formamide (DMF), esters and the like, the water cannot directly enter the existing biochemical treatment system of an enterprise, and the wastewater can be continuously treated only by diluting COD (chemical oxygen demand) of the wastewater to 8000mg/L, so that the production capacity of the enterprise is limited in environmental protection. General water quality information is as follows:
| index of water quality | COD | NH3-N | TP | pH | TDS |
| Content (wt.) | 28000(mg/L) | 1900(mg/L) | 2.4(mg/L) | 5 | 0.46％ |
By analyzing the production process of a workshop and the data of the wastewater, a technical team thinks that the wastewater has feasibility of bacterial biochemistry although containing a large amount of difficult biological toxic substances and having higher organic matter concentration.
The process design comprises the following steps: according to the physiological characteristics of the mixed strain CHMS, a corresponding process, a thallus separation process and matched facilities and equipment are designed. And finally, directly carrying out bacterial biochemical treatment on the rifamycin-containing wastewater by using mixed strains.
The specific method comprises the following steps:
the method comprises the following steps: selecting mixed strain CHMS as initial biochemical inoculum. The mixed strain CHMS comprises the following components: dysgonomonas (33%), brevundimonas (31%), sphingobacterium (10%), erysipelothrix (9%), pseudochrobacterum (6%), pseudomonas (4%), vagococcus (4%), paracoccus (3%).
Step two: pumping the wastewater into a water quality adjusting tank, leading the wastewater into a biochemical treatment tank after water quality adjustment, adding urea and phosphorus salt into the biochemical treatment tank, and uniformly mixing to obtain mixed strain CHMS with the molar ratio of C to N to P being 200:5:1 and 15w/v% in the wastewater; earlier stage COD =6000mg/L low-load intermittent water inlet, DO is controlled at 3mg/L and the temperature is controlled at 25 ℃; after the concentration of the thalli in the biochemical treatment tank is stable and the removal rate of the thalli to the wastewater CODcr reaches more than 40%, carrying out high-load continuous water inflow with COD = i5000mg/L, and entering an operation control stage; in the stage, CODcr and NH3-N indexes of inlet water and outlet water of the biochemical treatment tank are monitored every day to detect the biological pretreatment effect until the removal rate of CODcr of wastewater is more than 60%.
In the second step, an aeration system is arranged at the bottom of the biochemical treatment tank, and wastewater enters the bottom of the biochemical treatment tank and exits from the upper part of the biochemical treatment tank; the biochemical treatment pond lateral wall is equipped with the mud discharging mouth, and the terminal play water of waste water treatment inserts MBR membrane (polyvinylidene fluoride hollow fiber membrane, and the membrane aperture is 0.2 micron) processing module, carries out MBR membrane treatment to the suspension thalli that holds back MBR membrane flows back to in the biochemical treatment pond.
Step three: growth factors required for growth of dominant bacteria are added into the wastewater to optimize the pretreatment effect. The growth factors comprise vitamins, trace elements, biotin and the like, and comprise the following specific components:
step four: taking wastewater after the system stably operates, extracting microbial genome DNA, performing high-throughput sequencing analysis on a microbial structure in the biochemical system, and determining the proportion of genus and dominant genus in the biochemical system; the dominant genera are the top 2 genera, dysgonomonas (40.5%) and Brevundimonas (36.16%) in proportion, and the sum of the abundances of the dominant genera exceeds 50%.
Step five: in the operation process, when the treatment effect is not good, the ratio of each genus in the inoculated mixed strain CHMS is adjusted to be consistent with the ratio of the genus in the step four according to the genus ratio result in the step four. The mark with poor treatment effect is as follows: the removal rate of CODcr of the wastewater is lower than 40 percent or the concentration of the thalli in the microscopic bacterial liquid is lower than 50 percent of the concentration of the thalli under the normal treatment effect.
Step six: the main indexes after treatment are as follows: the integral retention time is 7 days, as shown in figures 1 and 2 (the abscissa in the figures is the operation time, and the ordinate is mg/L), the removal rate of the water CODcr reaches more than 60%, the total nitrogen removal rate reaches more than 85%, the system operation is very stable, the volatility is small, the burden of a subsequent system is greatly reduced, and the wastewater treatment capacity of the whole sewage system is improved by 50%.
Example 2
The method comprises the following steps: selecting mixed strain CHMS as initial biochemical bacterial agent. The mixed strain CHMS comprises the following components: dysgonomonas (28%), brevundimonas (36%), sphingobacterium (10%), erysipelothrix (6%), pseudochrobacterum (5%), pseudomonas (3%), vagococcus (3%), paracoccus (3%).
Step two: pumping the wastewater into a water quality adjusting tank, leading the wastewater into a biochemical treatment tank after water quality adjustment, adding urea and phosphorus salt into the biochemical treatment tank, and uniformly mixing to ensure that the molar ratio of C to N to P in the wastewater is 210: 6: 1 and 20w/v% of mixed strain CHMS; earlier stage COD =8000mg/L low-load intermittent water inlet, DO is controlled at 4mg/L, and the temperature is controlled at 28 ℃; after the concentration of thalli in the biochemical treatment tank is stable and the removal rate of the thalli to the CODcr of the wastewater reaches more than 40%, carrying out high-load continuous water inflow with COD =20000mg/L, and entering an operation control stage; in this stage, CODcr and NH3-N indexes of inlet water and outlet water of biochemical treatment tank are monitored every day to detect biological pretreatment effect until the CODcr removing rate of waste water is above 60%.
In the second step, an aeration system is arranged at the bottom of the biochemical treatment tank, and wastewater enters the bottom of the biochemical treatment tank and exits from the upper part of the biochemical treatment tank; the side wall of the biochemical treatment tank is provided with a sludge discharge port, the effluent at the tail end of the wastewater treatment is connected into an MBR (polyvinylidene fluoride hollow fiber membrane with the membrane aperture of 0.2 micron) treatment module, the MBR membrane is treated, and suspended bacteria intercepted by the MBR membrane flow back to the biochemical treatment tank.
Step three: growth factors required for growth of dominant bacteria are added into the wastewater to optimize the pretreatment effect. The growth factors comprise vitamins, trace elements, biotin and the like, and comprise the following specific components:
step four: taking the wastewater after the system stably runs, extracting microbial genome DNA, performing high-throughput sequencing analysis on microbial structures in the biochemical system, and determining the genus proportion and dominant genus in the wastewater of the biochemical system finally; the dominant genera are the top 2 genera, dysgonomonas (38.61%), brevundimonas (33.51%) and Sphingobacterium (16.52%) in proportion, and the sum of the abundances of the dominant genera exceeds 50%.
Step five: in the operation process, when the treatment effect is not good, the ratio of each genus in the inoculated mixed strain CHMS is adjusted to be consistent with the ratio of the genus in the step four according to the genus ratio result in the step four. The mark with poor treatment effect is as follows: the removal rate of CODcr of the wastewater is lower than 40 percent or the concentration of the thalli in the microscopic bacterial liquid is lower than 50 percent of the concentration of the thalli under the normal treatment effect.
Step six: the main indexes after treatment are as follows: the integral retention time is several days, the removal rate of the CODcr of the water quality reaches more than 65%, the removal rate of the total nitrogen reaches more than 90%, the system is very stable in operation and small in fluctuation, the burden of a subsequent system is greatly reduced, and the wastewater treatment capacity of the whole sewage system is improved by 60%.
Example 3
The method comprises the following steps: selecting mixed strain CHMS as initial biochemical bacterial agent. The mixed strain CHMS comprises the following components: dysgonomonas (30%), brevundimonas (34%), sphingobacterium (10%), erysipelothrix (6%), pseudochrobacterum (7%), pseudomonas (6%), vagococcus (4%), paracoccus (3%).
Step two: pumping the wastewater into a water quality adjusting tank, leading the wastewater into a biochemical treatment tank after water quality adjustment, adding urea and phosphorus salt into the biochemical treatment tank, and uniformly mixing to ensure that the molar ratio of C to N to P in the wastewater is 210: 6: 1 and 20w/v% of mixed strain CHMS; the early COD =10000mg/L low-load intermittent water inlet, and the DO is controlled at 5mg/L and the temperature is controlled at 25 ℃; after the concentration of the thalli in the biochemical treatment tank is stable and the removal rate of the thalli to the wastewater CODcr reaches more than 40%, carrying out high-load continuous water inflow with COD =25000mg/L, and entering an operation control stage; in this stage, CODcr and NH3-N indexes of inlet water and outlet water of biochemical treatment tank are monitored every day to detect biological pretreatment effect until the CODcr removing rate of waste water is above 60%.
In the second step, an aeration system is arranged at the bottom of the biochemical treatment tank, and wastewater enters the bottom of the biochemical treatment tank and exits from the upper part of the biochemical treatment tank; the side wall of the biochemical treatment tank is provided with a sludge discharge port, the effluent at the tail end of the wastewater treatment is connected into an MBR (polyvinylidene fluoride hollow fiber membrane with the membrane aperture of 0.2 micron) treatment module, the MBR membrane is treated, and suspended bacteria intercepted by the MBR membrane flow back to the biochemical treatment tank.
Step three: growth factors required for growth of dominant bacteria are added into the wastewater to optimize the pretreatment effect. The growth factors comprise vitamins, trace elements, biotin and the like, and comprise the following specific components:
step four: taking wastewater after the system operates stably, extracting microbial genome DNA, performing high-throughput sequencing analysis on microbial structures in the biochemical system, and determining the proportion of genus and dominant genus in the wastewater of the biochemical system finally; the dominant genera are the top 2 genera, dysgonomonas (40.61%), brevundimonas (38.51%) and Sphingobacterium (14.52%) in proportion, and the sum of the abundances of the dominant genera exceeds 50%.
Step five: in the operation process, when the treatment effect is not good, the ratio of each genus in the inoculated mixed strain CHMS is adjusted to be consistent with the ratio of the genus in the step four according to the genus ratio result in the step four. The mark with poor treatment effect is as follows: the removal rate of CODcr of the wastewater is lower than 40 percent or the concentration of the thalli in the microscopic bacterial liquid is lower than 50 percent of the concentration of the thalli under the normal treatment effect.
Step six: the main indexes after treatment are as follows: the integral retention time is several days, the removal rate of the CODcr of the water quality reaches more than 65%, the removal rate of the total nitrogen reaches more than 85%, the system is very stable in operation and small in fluctuation, the burden of a subsequent system is greatly reduced, and the wastewater treatment capacity of the whole sewage system is improved by 55%.
The raw materials and equipment used in the invention are common raw materials and equipment in the field if not specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, alterations and equivalents of the above embodiments according to the technical spirit of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (9)
1. A method for pretreating rifamycin-containing wastewater by microorganisms is characterized by comprising the following steps:
the method comprises the following steps: selecting a mixed strain as an initial biochemical microbial inoculum; the mixed strain comprises:Dysgonomonas30 to 33 percent of bacteria genus,Brevundimonas29 to 32 percent of brevundimonas,Sphingobacterium8-12% of Sphingobacterium,Erysipelothrix5-9% of erysipelothrix, and the rest of other genera; said other genera includePseudochrobactrum4 to 8 percent of the genus Pseudochrobacter,Pseudomonas2 to 6 percent of pseudomonas,Vagococcus3-5% of the genus roaming coccus,Paracoccus2-4% of Paracoccus;
step two: adding the mixed strain into a wastewater biochemical system containing rifamycin wastewater to carry out bacterial biochemical treatment;
step three: adding growth factors required by growth of dominant bacteria into the wastewater to optimize the pretreatment effect;
step four: taking a bacterium solution after a wastewater biochemical system is stabilized, extracting microbial genome DNA, performing high-throughput sequencing on a microbial structure in the wastewater biochemical system, and determining a final genus proportion and a dominant genus;
step five: in the operation process, when the treatment effect is not good, the ratio of each genus in the inoculated mixed strain is adjusted to be consistent with the ratio of the genus in the step four according to the result of the genus ratio in the step four.
2. The method of claim 1, wherein: the microorganism pretreatment method is suitable for rifamycin-containing wastewater meeting the following indexes: the proportion of the biochemical organic carbon in the wastewater accounts for more than 50 percent of the total organic carbon, the ratio of BOD5/CODcr is 0.4 +/-0.1, and the CODcr is 10000 to 20000mg/L.
3. The method of claim 1 or 2, wherein: the second step specifically comprises: transferring the wastewater to a water quality adjusting tank, leading the wastewater to a biochemical treatment tank after water quality adjustment, and adding urea, phosphate and 15-25w/v% of mixed strains into the biochemical treatment tank; the early COD =5000-10000mg/L low-load intermittent water inlet, the DO is controlled at 2-5mg/L and the temperature is controlled at 25-30 ℃; after the concentration of the thalli in the biochemical treatment tank is stable and the removal rate of the thalli to the wastewater CODcr reaches more than 40%, carrying out high-load continuous water inflow with COD =10000-20000mg/L, and entering an operation control stage; CODcr and NH of inlet water and outlet water of biochemical treatment tank in this stage 3 And monitoring the-N index to detect the biochemical treatment effect until the removal rate of the CODcr of the wastewater is more than 60%.
4. The method of claim 3, wherein: in the second step, urea and phosphorus salt are added to ensure that C in the wastewater is: n: the molar ratio of P is 200:5:1.
5. the method of claim 3, wherein: in the second step, an aeration system is arranged at the bottom of the biochemical treatment tank, wastewater enters the bottom of the biochemical treatment tank, and water exits from the upper part of the biochemical treatment tank; the side wall of the biochemical treatment tank is provided with a sludge discharge port, the effluent of the biochemical treatment tank is connected with an MBR membrane treatment module for MBR membrane treatment, and suspended bacteria intercepted by the MBR membrane flow back to the biochemical treatment tank.
6. The method of claim 5, wherein: in the second step, the MBR membrane is a polyvinylidene fluoride hollow fiber membrane with the membrane aperture of 0.2 micron.
7. The method of claim 1, wherein: in the third step, the growth factors are vitamins, trace elements and biotin.
8. The method of claim 1, wherein: in step four, the dominant genera are the top-ranked ones with abundance, and the sum of the abundances of the dominant genera exceeds 50%.
9. The method of claim 1, wherein: in the fifth step, the mark with poor treatment effect is as follows: the removal rate of CODcr of the wastewater is lower than 40 percent, or the concentration of the bacteria in the bacteria liquid is lower than 50 percent of that of the bacteria when the normal treatment efficiency is achieved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210139514.XA CN114620841B (en) | 2022-02-15 | 2022-02-15 | Microbial pretreatment method for rifamycin-containing wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210139514.XA CN114620841B (en) | 2022-02-15 | 2022-02-15 | Microbial pretreatment method for rifamycin-containing wastewater |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114620841A CN114620841A (en) | 2022-06-14 |
| CN114620841B true CN114620841B (en) | 2022-12-09 |
Family
ID=81897821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210139514.XA Active CN114620841B (en) | 2022-02-15 | 2022-02-15 | Microbial pretreatment method for rifamycin-containing wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114620841B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013179116A1 (en) * | 2012-05-31 | 2013-12-05 | Uniwersytet Warszawski | Composition of bacterial strains, bioremediation mixture and use of this composition for the removal of contaminants from the soil and a method for purifying of the soil contaminants |
| CN106746155A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of processing method of Lincomycin wastewater |
| CN106746161A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of rifamycin produces the processing method of waste water |
| CN109250805A (en) * | 2018-09-05 | 2019-01-22 | 太原理工大学 | It is a kind of while handling high concentration starch water and optical microbial fuel cell and its application without carbon source containing nitrate wastewater |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
-
2022
- 2022-02-15 CN CN202210139514.XA patent/CN114620841B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013179116A1 (en) * | 2012-05-31 | 2013-12-05 | Uniwersytet Warszawski | Composition of bacterial strains, bioremediation mixture and use of this composition for the removal of contaminants from the soil and a method for purifying of the soil contaminants |
| CN106746155A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of processing method of Lincomycin wastewater |
| CN106746161A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of rifamycin produces the processing method of waste water |
| CN109250805A (en) * | 2018-09-05 | 2019-01-22 | 太原理工大学 | It is a kind of while handling high concentration starch water and optical microbial fuel cell and its application without carbon source containing nitrate wastewater |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
Non-Patent Citations (2)
| Title |
|---|
| 有机化工废水COD高效降解菌的分离筛选及应用;张为艳等;《工业水处理》;20160831;第36卷(第8期);第52-54页 * |
| 生物强化技术处理利福霉素生产废水的研究;沈永红等;《广西师范大学学报(自然科学版)》;20050625;第23卷(第02期);第90-93页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114620841A (en) | 2022-06-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110156174B (en) | Mixed strain fermentation biological pretreatment method for high-concentration high-salt pharmaceutical wastewater | |
| Shi et al. | Performance of halophilic marine bacteria inocula on nutrient removal from hypersaline wastewater in an intermittently aerated biological filter | |
| CN105645710B (en) | A kind of method that mud decrement is carried out using complex microorganism preparations | |
| CN105907673A (en) | Method for strengthening biochemical water treatment effects by microbial preparation | |
| CN110699285B (en) | Palyalisma and application thereof in treating landfill leachate membrane concentrated solution | |
| CN106676022A (en) | Method for preparing microbe bacteria liquid for treating black-odor riverway | |
| CN109868233B (en) | Efficient special microbial inoculum for acidic water and application method thereof | |
| CN103708610A (en) | Culture method of activated sludge for coking wastewater treatment | |
| CN106434469B (en) | Low-temperature-resistant nitrifying bacteria agent and preparation method and application thereof | |
| CN109052654A (en) | A method of for improving the smelly water of urban black | |
| Ma et al. | Cooperation triggers nitrogen removal and algal inhibition by actinomycetes during landscape water treatment: performance and metabolic activity | |
| CN109081447B (en) | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas | |
| CN109439569A (en) | Heterotrophic nitrification-biological aerobic denitrification comamonas, the liquid bacterial agent containing the bacterium and its application in membrane bioreactor | |
| CN108342338B (en) | Method for treating pharmaceutical wastewater containing antibiotics | |
| CN114620841B (en) | Microbial pretreatment method for rifamycin-containing wastewater | |
| CN117511782A (en) | Screening and domesticating method for aerobic denitrifying bacteria for treating low-C/N sewage | |
| CN112047468A (en) | Biochemical treatment method of landfill leachate | |
| CN114605030B (en) | Method for recycling carbon-sink oxygen-release type cultivation sewage | |
| CN106277286B (en) | Method for eliminating viscous swelling of activated sludge in biochemical system | |
| CN112358041B (en) | Granular sludge culture method for synchronous denitrification and methane production and COD removal | |
| CN109110927B (en) | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella and acinetobacter | |
| CN110697883B (en) | Method for strengthening denitrification function of membrane bioreactor and slowing down membrane pollution | |
| CN116354518A (en) | Method for pretreating gentamicin-containing wastewater by using mixed flora | |
| Wang et al. | Microbial community characteristics and shortcut nitrification-denitrification under the intermittent aeration profile in a Sbr treating swine wastewater | |
| CN114684924B (en) | Denitrification treatment method for salt-containing wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |