CN104877916A - Paracoccus limosus, bacterium thereof, method for preparing bacterium and application thereof - Google Patents
Paracoccus limosus, bacterium thereof, method for preparing bacterium and application thereof Download PDFInfo
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Abstract
The invention discloses paracoccus limosus, a bacterium thereof, a method for preparing the bacterium and application thereof. The paracoccus limosus is paracoccus limosus A36, is preserved in preservation authority which is the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms on November 21st, 2014, and the preservation number of the paracoccus limosus is CGMCC No.10032; the paracoccus limosus belongs to paracoccus versutus of rhodobacteraceae of rhodobacterales of alpha-proteobacteria of proteobacteria of bacteria. The radial paracoccus limosus is cultivated on a plate, and seeds are cultivated and fermented to obtain the bacterium with the paracoccus limosus which is an active ingredient. The method for preparing the bacterium with bacillus which is an active ingredient includes steps of cultivating the paracoccus limosus on the plate; cultivating the seeds; fermenting the seeds; preparing the bacterium. The bacterium can be applied to preparing basic fertilizers with functions of dissolving phosphorus and potassium and increasing quick-acting nitrogen in soil.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of secondary coccus and microbial inoculum thereof and preparation method and application.
Background technology
Using in a large number along with chemical fertilizer in modern agricultural production, the output value, the output of farm crop are all greatly improved, and result also in degradation series of problems under soil organism reduction, soil compaction, fertility simultaneously.Soil microorganisms has the effect promoting Soil Nutrient Transformation, activating soil nutrient, increase nutrient supply.Soil microorganisms participates in the various biochemical reactions of Soil Nutrient Transformation, with the supply of soil nutrient with lose closely related.In soil, the processes such as organic matter mineralization and humify, the nitrification and denitrification of nitrogen, the effectuation of phosphorous potassium mineral all have the participation of soil microorganisms, soil microorganisms governs Soil Nitrogen, phosphorus, the supply of potassium nutrition element and biological effectiveness, affects the quality of cigarette strain.And soil nutrient major part exists with invalid state, nitrogen major part is organonitrogen, and phosphorous and potassium-bearing mineral accounts for more than 95% of total Phosphorus In Soil potassium.Organonitrogen mineralising under the effect of microorganism is inorganic nitrogen, supply plant nutrition; Molten phosphorus silicate-dissolving microbe in soil can secrete formic acid, acetic acid, oxalic acid, citric acid, succinic acid etc., dissolves phosphorous and potassium-bearing mineral, and release phosphorus potassium, improves their biological effectiveness, improve crop alimentary.In soil, there is the multiple nitrogen-fixing microorganisms such as symbiotic nitrogen-fixing bacteria, azotobacter and combination azotobacter, by the nitrogen in different modes fixed air, increase the nitrogen nutrient of soil, the needs of supply plant nutrition.And the microbial inoculum that a kind of microorganism has multiple effect has no relevant report.
Summary of the invention
The present invention first object is to provide a kind of secondary coccus, second object is to provide a kind of microbial inoculum using described secondary coccus as activeconstituents, 3rd object is to provide described using secondary coccus as the bacterial preparation process of activeconstituents, and the 4th object is the application providing described microbial inoculum.
The present invention first object realizes like this, described secondary coccus is paracoccus (Paracoccus limosus) A36, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preservation day: on November 21st, 2014, deposit number: CGMCC No. 10032; Described paracoccus in bacterium circle, Proteobacteria, α-distortion Gammaproteobacteria, red bacillus order, red Bacteriaceae, paracoccus.
---the acquisition of paracoccus (Paracoccus limosus) A36 and qualification
(1) acquisition of paracoccus (Paracoccus limosus) A36 and qualification
Secondary coccus of the present invention, adopt silicate bacteria substratum to separate from the purple soil of the yellow official's vega in great Ying town, Yuxi Hongta District, silicate bacteria substratum is selected the bacterium colony that bacterium colony is transparent, shinny, viscosity is larger be transferred on inorganic phosphorus bacteria substratum again, after there is molten phosphorus circle, record colony diameter and molten phosphorus circle size, get the larger bacterium of molten phosphorus circle again purifying cultivate, then in the sterilizing drier oil pipe choosing 40% 4 DEG C save backup.
Used medium is filled a prescription:
1, silicate bacteria substratum:
Sucrose 5g, soil mineral 1.0g, MgSO
47H
2o 0.5g, CaCO
30.1g, Na
2hPO
42.0g, FeCl
3(1%) 0.2ml, agar 20g, distilled water 1000ml, pH 7.0 ~ 7.5.
2, inorganic phosphorus bacteria substratum:
Glucose 10 g, (NH
4)
2sO
40.5 g, NaCl 0.3 g, KCl 0.3 g, MgSO
47H
2o 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o 0.03 g, CaCO
35 g, Ca
3(PO
4)
210g, agar 18-20g, distilled water 1000ml, pH 7.0 ~ 7.5.
To the strains A 36 of above-mentioned separation,
Carry out biology and physio-biochemical characteristics detection and molecular biology method by ordinary method to identify.Molecular assay method is as follows: the extraction TaKaRa MiniBEST Bacterial Genomic DNA Extraction Kit Ver.2.0 of bacterial genomes DNA, method is see test kit specification sheets.Pcr amplification selects primers F 27/R1492, and normal condition increases, and amplified production entrusts Shanghai Ying Jun Bioisystech Co., Ltd to carry out sequencing.
This bacterium is shaft-like, and without gemma, Gram-negative, glucose fermentation is negative, and catalase is positive, oxidase positive, and methyl red test is negative, VP negative, and nitrate reduction is positive..
16S rDNA sequential analysis: be paracoccus (Paracoccus limosus) by A36 identification of strains by sequence alignment and physio-biochemical characteristics.
(2) preservation of paracoccus (Paracoccus limosus) A36
By above-mentioned qualification result, confirm the strain of bacterial strain NB 88 (T) for the secondary coccus (Paracoccus limosus) of radial pattern, called after A36, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 21st, 2014 and (be called for short CGMCC, address is: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100080), its deposit number is CGMCC No. 10032.
The present invention second object is achieved in that the microbial inoculum made after slat chain conveyor, seed culture, fermentation with described NB88 (T).
The third object of the present invention is achieved in that and comprises slat chain conveyor, seed culture, fermentation, microbial inoculum preparation process, specifically comprises:
A, slat chain conveyor: be inoculated on plate culture medium by paracoccus (Paracoccus limosus) A36, cultivate 48 ~ 54h, obtain dull and stereotyped bacterial classification at temperature is 26 ~ 30 DEG C;
B, seed culture: by dull and stereotyped strain inoculation in liquid nutrient medium, at temperature is 26 ~ 30 DEG C, 55 ~ 65h cultivated by shaking table, obtains liquid fermenting seed;
C, fermentation: liquid fermenting seed is inoculated into fermentor tank by the amount of fermention medium volume 8 ~ 12%, 36 ~ 54h is cultivated at 28 ~ 32 DEG C, stirring velocity is 170 ~ 190 r/min, air flow 60 ~ 100%, obtains paracoccus (Paracoccus limosus) A36 fermented liquid;
Prepared by D, microbial inoculum: in fermented liquid, add adsorbent be less than 13% to moisture content weight ratio obtain target solids microbial inoculum through air-dry.
The present invention the 4th object is achieved in that the application of described microbial inoculum in preparation phosphorus decomposing, potassium decomposing, raising Soil Available nitrogen base manure.
Except as otherwise noted, the percentage ratio adopted in the present invention is percent by volume.
Secondary coccus of the present invention and with the advantage of the described secondary coccus preparation that is activeconstituents:
The present invention secondary coccus used has resolves into the inorganic phosphorus of indissoluble in soil, potassium phosphorus, the potassium that plant is easy to absorb, improve the content of full nitrogen in soil simultaneously, nitrogen, phosphorus, the potassium nutrition that soil can be promoted directly to discharge after using secondary coccus microbial inoculum not to be absorbed by plants, suitably reduce applying of inorganic N,P,K fertilizers; This bacterium is separated and obtains from vega soil simultaneously, applies field by the situation again with fertilizer after multiplication culture, has good potential using value to tobacco-growing soil improving of environment.
Accompanying drawing explanation
Fig. 1 is present invention process schema.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or improvement, all fall into protection scope of the present invention.
Secondary coccus of the present invention is paracoccus (Paracoccus limosus) A36, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preservation day: on November 21st, 2014, deposit number: CGMCC No.10032; Described paracoccus in bacterium circle, Proteobacteria, α-distortion Gammaproteobacteria, red bacillus order, red Bacteriaceae, paracoccus.
Secondary coccus of the present invention is the microbial inoculum of activeconstituents, is the microbial inoculum made after slat chain conveyor, seed culture, fermentation with described paracoccus (Paracoccus limosus) A36.
Secondary coccus of the present invention is the preparation method of the microbial inoculum of activeconstituents, comprises slat chain conveyor, seed culture, fermentation, microbial inoculum preparation process, specifically comprises:
A, slat chain conveyor: be inoculated on plate culture medium by paracoccus (Paracoccus limosus) A36, cultivate 48 ~ 54h, obtain dull and stereotyped bacterial classification at temperature is 26 ~ 30 DEG C;
B, seed culture: by dull and stereotyped strain inoculation in liquid nutrient medium, at temperature is 26 ~ 30 DEG C, 55 ~ 65h cultivated by shaking table, obtains liquid fermenting seed;
C, fermentation: liquid fermenting seed is inoculated into fermentor tank by the amount of fermention medium volume 8 ~ 12%, 36 ~ 54h is cultivated at 28 ~ 32 DEG C, stirring velocity is 170 ~ 190 r/min, air flow 60 ~ 100%, obtains paracoccus (Paracoccus limosus) A36 fermented liquid;
Prepared by D, microbial inoculum: in fermented liquid, add adsorbent be less than 13% to moisture content weight ratio obtain target solids microbial inoculum through air-dry.
Described plate culture medium is nutrient agar medium NA substratum, and its composition is: peptone 8 ~ 12g, beef powder 4 ~ 6g, sodium-chlor 4 ~ 6g, agar powder 16 ~ 20g, and all the other are water, and pH value is 7.0 ~ 7.5.
Described liquid nutrient medium and fermention medium are NB substratum, and its composition is peptone 8 ~ 12g, beef powder 4 ~ 6g, sodium-chlor 4 ~ 6g, and all the other are water, and pH value is 7.0 ~ 7.5.
The rotating speed of shaking table described in described step B is 100 ~ 110r/min.
In described step C during fermentation culture, charging capacity is 70 ~ 80% of fermentor tank.
In step C during fermentor cultivation, leavening temperature is 30 DEG C, and pressure is 0.05MPa, and every 2h monitoring once, stirring velocity is 170 ~ 190r/min, and incubation time is 48h.
In described D step, sorbent material is one or more of oil cake after diatomite, the peat composed of rotten mosses, fermentation or gac, and the particle diameter of sorbent material is 1 ~ 5mm.
Of the present inventionly be applied as described microbial inoculum in preparation phosphorus decomposing, potassium decomposing, application in raising Soil Available nitrogen base manure.
Be illustrated below by embodiment:
embodiment 1
---take paracoccus (Paracoccus limosus) A36 as the preparation of the preparation of activeconstituents
Be taken at cultured paracoccus Paracoccus limosus on NA flat board to be inoculated into and to be equipped with in the 250ml triangular flask of NB liquid medium, be the shaking table of 100-110 rev/min is cultivated after 60 hours at rotating speed, inoculum size by 10% is transferred in the group training orchid bottle that liquid medium is housed again, be the shaking table of 100-110 rev/min is cultivated after 72 hours at rotating speed, adsorb with the peat composed of rotten mosses 80L that particle diameter is 1-5mm, form solid fungicide type.
Above-described NA medium component is: peptone 10.0g, beef powder 5.0g, sodium-chlor 5.0g, agar powder 15.0g, distilled water 1000m, pH7.0-7.5.NB nutrient solution composition is: peptone 10.0g, beef powder 5.0g, sodium-chlor 5.0g, distilled water 1000m, pH7.0-7.5.
Above culture temperature is room temperature 20-25 DEG C, and shaking speed is 100-110 rev/min, and incubation time is 72 hours.
embodiment 2
---take paracoccus (Paracoccus limosus) A36 as the preparation of the preparation of activeconstituents
Being taken at cultured paracoccus Paracoccus limosus on NA flat board is inoculated in the group training orchid bottle that NB liquid medium is housed, be the shaking table of 100-110 rev/min is cultivated after 60 hours at rotating speed, inoculum size by 10% is transferred to the CT5-2 that liquid fermentation and culture liquid is housed again and automatically ferments in filling, charging capacity is 3.5L, fermented incubation time is 48 hours, adsorb with the peat composed of rotten mosses 80L that particle diameter is 1-5mm, form solid fungicide type.
Above-described NA medium component is: peptone 10.0g, beef powder 5.0g, sodium-chlor 5.0g, agar powder 15.0g, distilled water 1000m, pH7.0-7.5.NB nutrient solution composition is: peptone 10.0g, beef powder 5.0g, sodium-chlor 5.0g, distilled water 1000m, pH7.0-7.5.
The CT5-2 air flow of sterile air of filling with that automatically ferments is: 1:10; Stirring velocity is 170-190 rev/min, and culture temperature is: 30 DEG C, incubation time 48 hours.
embodiment 3
---with paracoccus (Paracoccus limosus) A36 fungi degradation phosphorus, potassium decomposing, improve the test experiments of Soil Nitrogen ability
1. liquid culture method measures the ability of dissolving potassium of bacterial strain
Separate in bottle at 100ml tri-and add 20mlNB nutrient solution, adding soil mineral 0.2g, after sterilizing, every bottle adds paracoccus Paracoccus limosus liquid bacteria liquid 1ml, establish the bacterium liquid do not inoculated to compare simultaneously, each process repeats for three times, constant-temperature shaking culture 6 days under 28 DEG C of conditions, gets centrifugal bacterium liquid (supernatant liquor) 10ml, adds the H of 2ml 6%
2o
2, in boiling water bath, digest 1h, distilled water is settled to 50ml, uses flame spectrophotometer measuring potassium concentration.
2. flat band method measures the dissolving P capacity of bacterial strain
Phosphate solubilizing bacteria is cultivated on the solid medium of organophosphorus, measures the size that periphery of bacterial colonies produces molten phosphorus circle.
3. soil incubation method measures the ability of the potassium decomposing of bacterial strain, phosphorus decomposing and raising total soil nitrogen
Take 20g soil (fine earth) to load in 500mL group training orchid bottle and to add sterile purified water 200 m L, every strain bacterium inoculates 3 bottles, every bottle graft kind 10mL bacteria culture fluid, separately get 3 bottles of aqua sterilisas adding equivalent in contrast 1, separately get 3 bottles of sterilizing NB nutrient solutions adding equivalent and compare 2, put constant indoor temperature shaking table and cultivate 15d, shaking speed is 110 revs/min, after cultivation terminates, measure soil filtered liquid effective K, rapid available phosphorus and total nitrogen content.
4. pot-culture method measures the ability of the potassium decomposing of bacterial strain, phosphorus decomposing and raising total soil nitrogen
Be load red soil in the flowerpot of 30cm at diameter, the paracoccus Paracoccus limosus bacterium solid fungicide getting one glass of peat composed of rotten mosses absorption with the beaker of 150ml applies in flowerpot, every basin uses one glass, in solid fungicide and flowerpot, the soil of 15cm mixes, then every potted plant cigarette one strain, set the peat composed of rotten mosses not adding bacterium liquid as contrasting simultaneously, cigarette 10 basin is planted in each process, totally 20 basins, normally water, no longer use any fertilizer, plant the biological character that cigarette measures cigarette strain for 40 days afterwards, simultaneously each process measures the soil organism after getting 5 basins soil mixing, full N, P, K and available N, P, K.
5. results and analysis
5.1 liquid culture methods measure the ability of dissolving potassium of paracoccus Paracoccus limosus A36 bacterial strain
The soil mineral of paracoccus Paracoccus limosus NB88 (T) bacterial strain to indissoluble has good potassium decomposing effect (table 1), and its ability of dissolving potassium improves 1.76 times compared with the control.
Table 1 paracoccus Paracoccus limosus A36 bacterial strain is to the potassium decomposing effect of soil mineral
| Process | K concentration (mg/l) | 5% conspicuous level | 1% pole conspicuous level |
| A36 | 2.70 | a | A |
| CK | 0.98 | b | B |
5.2 flat band methods measure the dissolving P capacity of paracoccus Paracoccus limosus A356 bacterial strain
The calcium phosphate of paracoccus Paracoccus limosus NB88 (T) bacterial strain to indissoluble does not have phosphorus decomposing effect (table 2), and five average D/d repeated are 0.
Table 2 paracoccus Paracoccus limosus A36 bacterial strain is to the phosphorus decomposing effect of calcium phosphate
5.3 soil incubation methods measure the ability of the potassium decomposing of paracoccus Paracoccus limosus A36 bacterial strains, phosphorus decomposing and raising total soil nitrogen
Paracoccus Paracoccus limosus A36 bacterial strain has the effect (table 3) of good phosphorus decomposing, potassium decomposing and raising total soil nitrogen and nitric nitrogen to red soil.Phosphorus decomposing effect improves 0.73 times compared with CK2, and potassium decomposing effect improves 0.2 times, and the total nitrogen in soil improves 0.33 times, and nitric nitrogen improves 0.71 times.
Table 3 paracoccus Paracoccus limosus A36 bacterial strain is to the effect of the phosphorus decomposing of soil, potassium decomposing and raising Soil Nitrogen
| Process | Rapid available phosphorus (mg/L) | Available potassium (mg/L) | Total nitrogen (mg/L) | Nitric nitrogen (mg/L) |
| Paracoccus Paracoccus limosus A36 bacterium | 0.038 | 22.47 | 42.00 | 26.1 |
| CK2(connects NB aseptic culture fluid) | 0.022 | 18.73 | 31.47 | 15.3 |
| CK1(connects sterile purified water) | 0.020 | 6.11 | 1.34 | 0.9 |
5.4 pot-culture method methods measure the ability of the potassium decomposing of paracoccus Paracoccus limosus A36 bacterial strains, phosphorus decomposing and raising total soil nitrogen
Use paracoccus Paracoccus limosus microbial inoculum and significantly (table 4, table 5) is not affected to cigarette strain growth, after cigarette strain transplants 40 days, use the cigarette strain plant height of paracoccus Paracoccus limosus A36 microbial inoculum than the high 0.6cm of contrast, leaf-area coefficient is than contrast few 0.0013.
Use nutrient in paracoccus Paracoccus limosus microbial inoculum soil to improve.After cigarette strain transplants 40 days, use the hydrolyzable nitrogen of paracoccus Paracoccus limosus microbial inoculum soil than contrast raising 37.4 mg/kg, available phosphorus improves 2.3mg/kg than contrast, available potassium improves 5mg/kg than contrast, full nitrogen is than contrast raising 0.008%, full phosphorus is than contrast raising 0.002%, and full potassium is than contrast raising 0.021%.
Table 4 paracoccus Paracoccus limosus A36 bacterial strain is on the impact of cigarette strain plant height
Table 5 paracoccus Paracoccus limosus A36 bacterial strain is on the impact of cigarette strain leaf-area coefficient
| Process | Strain 1 | Strain 2 | Strain 3 | On average |
| Paracoccus Paracoccus limosus A36 bacterium | 0.1889 | 0.1570 | 0.2247 | 0.1902Aa |
| CK | 0.1704 | 0.1800 | 0.2240 | 0.1915Aa |
Table 6 paracoccus Paracoccus limosus A36 bacterial strain is on the impact of soil nutrient
| Process | Organic (g/kg) | Hydrolyzable nitrogen (mg/kg) | Available phosphorus (mg/kg) | Available potassium (mg/kg) | Full nitrogen (%) | Full phosphorus (%) | Full potassium (%) |
| Paracoccus Paracoccus limosus A36 bacterium | 16.4 | 80.3 | 63.1 | 210 | 0.066 | 0.104 | 0.506 |
| CK | 16.2 | 42.9 | 60.8 | 205 | 0.058 | 0.102 | 0.485 |
Claims (9)
1. a secondary coccus, it is characterized in that described secondary coccus is paracoccus (Paracoccus limosus) A36, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preservation day: on November 21st, 2014, deposit number: CGMCC No. 10032; Described paracoccus in bacterium circle, Proteobacteria, α-distortion Gammaproteobacteria, red bacillus order, red Bacteriaceae, paracoccus.
2. secondary coccus according to claim 1 is a microbial inoculum for activeconstituents, it is characterized in that the microbial inoculum made after slat chain conveyor, seed culture, fermentation with described paracoccus (Paracoccus limosus) A36.
3. secondary coccus according to claim 2 is a preparation method for the microbial inoculum of activeconstituents, it is characterized in that comprising slat chain conveyor, seed culture, fermentation, microbial inoculum preparation process, specifically comprises:
A, slat chain conveyor: be inoculated on plate culture medium by paracoccus (Paracoccus limosus) A36, cultivate 48 ~ 54h, obtain dull and stereotyped bacterial classification at temperature is 26 ~ 30 DEG C;
B, seed culture: by dull and stereotyped strain inoculation in liquid nutrient medium, at temperature is 26 ~ 30 DEG C, 55 ~ 65h cultivated by shaking table, obtains liquid fermenting seed;
C, fermentation: liquid fermenting seed is inoculated into fermentor tank by the amount of fermention medium volume 8 ~ 12%, 36 ~ 54h is cultivated at 28 ~ 32 DEG C, stirring velocity is 170 ~ 190 r/min, air flow 60 ~ 100 %, obtains paracoccus (Paracoccus limosus) A36 fermented liquid;
Prepared by D, microbial inoculum: in fermented liquid, add adsorbent be less than 13% to moisture content weight ratio obtain target solids microbial inoculum through air-dry.
4. secondary coccus according to claim 3 is the preparation method of the microbial inoculum of activeconstituents, it is characterized in that described plate culture medium is nutrient agar medium NA substratum, its composition is: peptone 8 ~ 12g, beef powder 4 ~ 6g, sodium-chlor 4 ~ 6g, agar powder 16 ~ 20g, all the other are water, and pH value is 7.0 ~ 7.5.
5. secondary coccus according to claim 3 is the preparation method of the microbial inoculum of activeconstituents, it is characterized in that the rotating speed of shaking table described in described step B is 100 ~ 110r/min.
6. secondary coccus according to claim 3 is the preparation method of the microbial inoculum of activeconstituents, and when it is characterized in that fermentation culture in described step C, charging capacity is 70 ~ 80% of fermentor tank.
7. secondary coccus according to claim 3 is the preparation method of the microbial inoculum of activeconstituents, and when it is characterized in that fermentor cultivation in step C, leavening temperature is 30 DEG C, and pressure is 0.05MPa, and stirring velocity is 170 ~ 190r/min, and incubation time is 48h.
8. secondary coccus according to claim 3 is the preparation method of the microbial inoculum of activeconstituents, and it is characterized in that sorbent material in described D step is one or more of oil cake after diatomite, the peat composed of rotten mosses, fermentation or gac, the particle diameter of sorbent material is 1 ~ 5mm.
9. secondary coccus according to claim 2 is an application for the microbial inoculum of activeconstituents, it is characterized in that the application of described microbial inoculum in preparation phosphorus decomposing, potassium decomposing, raising Soil Available nitrogen base manure.
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| CN106746155B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Method for treating lincomycin production wastewater |
| CN106746161B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Method for treating rifamycin production wastewater |
| CN106834182A (en) * | 2017-02-27 | 2017-06-13 | 中国科学院成都生物研究所 | One plant of secondary meningitidis strains apt to change and its application |
| CN106834182B (en) * | 2017-02-27 | 2019-11-08 | 中国科学院成都生物研究所 | One plant of secondary meningitidis strains apt to change and its application |
| CN114410545A (en) * | 2022-02-23 | 2022-04-29 | 中国科学院微生物研究所 | Paracoccus strain GN-9 and application thereof |
| CN114410545B (en) * | 2022-02-23 | 2023-07-28 | 中国科学院微生物研究所 | Paracoccus GN-9 and application thereof |
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