CN108773912A - A kind of microbial inoculum treatment process of livestock and poultry cultivation sewage - Google Patents
A kind of microbial inoculum treatment process of livestock and poultry cultivation sewage Download PDFInfo
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- CN108773912A CN108773912A CN201810937601.3A CN201810937601A CN108773912A CN 108773912 A CN108773912 A CN 108773912A CN 201810937601 A CN201810937601 A CN 201810937601A CN 108773912 A CN108773912 A CN 108773912A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Health & Medical Sciences (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage, device includes：Aerobic seeding tank, anaerobic species tank, grid, regulating reservoir, flocculation basin, anaerobic reation pool, aerobic aeration biofilter, methane recovery device.Operating procedure includes：The configuration of culture medium；The culture of anaerobic bacteria；The culture of aerobic bacteria；The culture of flocculated bacteria；Strain expands, and strengthens, domestication culture；Sewage adjusts pH through grid, in regulating reservoir；Into flocculation basin, flocculation microbial inoculum is added；Sewage enters anaerobic reation pool and stops 8~10h, and methane is recycled in stirring；Sewage flows into aerobic aeration biofilter, by three layers of profiled filament nylon filtering cloth；Gravity secondary filter, ultraviolet lamp disinfection discharge after detection, and anaerobic reation pool is returned if not up to standard.Good sewage processing effect, no added chemical reagent avoid introducing secondary pollution；Ventilatory capacity, amount of agitation, drainage flow amount etc. are low, and foul smell and excess sludge discharge are few, realize energy-saving and emission-reduction.
Description
Technical field
The present invention relates to sewage treatment process, in particular to a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage.
Background technology
Trade effluent or municipal wastewater major pollutants are oxygen consumption pollutant（It is carbohydrate, protein, grease, wooden
The organic substances such as element）, toxic pollutant（Inorganic anion, organic agricultural chemicals etc.）, inorganic pollution（Acid, alkali, salt etc.）And cause of disease
Body pollution object.Traditional sewage water treatment method is stirring, aerating oxidation processing, without gas Anaerobic Treatment and precipitation, oxidation
Processing and Anaerobic Treatment are substantially exactly the process of microbial degradation water pollutant, are usually all natural strains or manually add
Add a small amount of strain, also needs to addition part chemical reagent according to pollutant kind, precipitation process mostly uses nature and stands or add
Flocculant precipitates.The inefficiency of strain treated sewage without screening and optimizing needs fiting chemical reagent or physical method, increases
The possibility for having added secondary pollution also improves time and the economic cost of waste water control.In addition, sewage disposal device is for sewage
Treatment effect no less important, most sewage disposal device capacity is small, running efficiency is low, pollutant process is not thorough, easily produces
The foul smell and sludge of raw secondary pollution.Therefore, the dominant bacteria for screening sewage disposal is developed mating advanced sewage disposal and is set
It is standby, it will help the ability and efficiency for improving existing sewage disposal solve the problems, such as sewage disposal.
Chinese patent（CN201810012965.0）A kind of wastewater treatment equipment uses anaerobic species and facultative aerobe kind or good
The mixture of oxygen strain can reach good wastewater treatment efficiency as microbial water-purifying agent treated sewage, but the flocculation used
Agent is inorganic flocculating agent and organic polymer coargulator, and secondary pollution may be caused to environment.Chinese patent
（CN201611182630.0）A kind of technique for treating industrial wastewater handles waste water using mix bacterium agent, but due to not carried out to strain
The ability of domestication, strain processing waste water lowly easily can make the waste water given off that processing standard be not achieved.
Invention content
In view of the deficiencies of the prior art, a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage, sewage disposal were provided
Effect is good, no added chemical reagent, avoids introducing secondary pollution；The recycling of the gases such as methane carries out the utilization of resources；Ventilatory capacity, stirring
Amount, drainage flow amount etc. are low, and foul smell and excess sludge discharge are few, realize energy-saving and emission-reduction.
Technical scheme is as follows：
（1）The culture of anaerobic bacteria, aerobic bacteria：The preparation of culture medium：Lactose 0.3~0.5%, sodium hydrogen selenite 0.3~0.8%, phosphorus
Sour disodium hydrogen 0.3~0.8%, l-cysteine 0.3~0.5%, peptone 1%, potassium dihydrogen phosphate 0.3~0.8%, remaining be water, adjust
It is 7.0~7.5 to save pH value, by 110~130 DEG C of 20~25min of high-temperature sterilization, obtains culture medium A, B, C；One is pressed in desinfection chamber
Certainty ratio is inoculated with anaerobic species to culture medium A, is inoculated with by a certain percentage in aerobic bacteria to culture medium B, cultivation temperature in desinfection chamber
24~32 DEG C, incubation time 24~50 hours；
（2）Strain domestication, hot housing：It is put into respectively from secondary sedimentation tank of sewage treatment work in aerobic strain pond and anaerobic species pond
The sludge 20~30% of acquirement, sodium hydrogen selenite 0.5%, lactose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 0.5~1%, Portugal
Grape sodium saccharate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2%, waste water, then add cultivate respectively
Aerobic strain and anaerobic species；The waste water of pond body product 40~45%, stirring are first put into strain pond；Pond body product 6 is added daily
~8% waste water, until reaching ordinary water level, tamed, strengthen after aerobic and anaerobic sludge, and launched together into anaerobism
In reaction tank；
（3）The culture of flocculated bacteria：It is inoculated with the strain of flocculation ability in desinfection chamber：Pseudomonas mendocina, Paecilomyces lilacinus,
Inoculative proportion is 7：3 to culture medium C, 24~32 DEG C of cultivation temperature, incubation time 24~50 hours；Expand hot housing again, expands
Big hot housing based component is：Sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride
0.5%, sodium gluconate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2%, it is 7.0 to adjust pH value
~7.5；Shaking table is opened, cultivation temperature is 30 DEG C, incubation time 48h；It is first high to will be enlarged by the strain cultures after hot housing
Speed centrifugation, it is flocculation microbial inoculum to obtain supernatant；
（4）Sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity；It enters back into and overregulates pond adjusting pH
It is 6~7.5；
（5）Sewage goes successively to flocculation basin, and 2~5% flocculation microbial inoculum of sewage volume, stirring is added, and hydraulic retention 7~9h removals are hanged
Float and some of the contaminants；Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is and water (flow) direction phase
The anti-gradient, to collect and be discharged precipitation；
（6）Flocculation basin supernatant enters anaerobic reation pool, and 8~10h of hydraulic retention is stirred, and good bacteria can consume extra oxygen point
Pollutant is solved, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, the methane recovery generated during this is extremely
Anaerobic reation pool connects methane recovery pond；
（7）Sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter
Cobblestone；Next it is the cross nylon filtering cloth of first layer, it is that special-shaped ceramics grow 3~5cm to place filtrate, and mix bacterium agent is being filtered
Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering；The second layer is trilobal weave Buddhist nun
Imperial filter cloth filtrate be special-shaped ceramics grow 1~3cm, third layer be pentagon weave nylon filtering cloth, activated carbon, 5~100 mesh, until under
Up from thick to thin；Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of activated carbon, by
It is arrived carefully to be thick up to lower, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally
It is discharged after detection, anaerobic reation pool is returned if not up to standard.Sewage disposal device includes：Aerobic seeding tank, anaerobic species tank, lattice
Grid, regulating reservoir, flocculation basin, anaerobic reation pool, aerobic aeration biofilter, methane recovery device.
As the present invention further explanation, anaerobic species be methane phase bacillus, methane phase coccus, Bacillus acidi lactici group
It closes.
As the present invention further explanation, methane phase bacillus, methane phase coccus, Bacillus acidi lactici inoculative proportion be 1：1：
2。
As the further explanation of the present invention, aerobic strain is sulfide-oxidizing enzyme, polyP bacteria, hydrolysis acidification bacterium, saccharomycete
Combination.
As the present invention further explanation, sulfide-oxidizing enzyme, polyP bacteria, hydrolysis acidification bacterium, saccharomycete inoculative proportion be
2：2：3：5.
As the further explanation of the present invention, sulfide-oxidizing enzyme is Thiobacillus thioxidans, grate sulfur thiobacillus, ferrous oxide sulphur bar
One or both of bacterium, thiobacillus denitrificans.
It is Acinetobacter calcoaceticus, Acinetobacter junii, Denitrifying Phosphate Accumulating Organisms, deep red red as the further explanation of the present invention
One or both of red ring bacterium.
As the further explanation of the present invention, hydrolysis acidification bacterium is Bacillus cercus, starch Clostridium, succinic acid are quasi-
One or both of bacillus.
As the further explanation of the present invention, step（2）In stir speed (S.S.) be 400rpm~600rpm；Step（3）In
Shaking table rate is 100~150r/min；Step（5）Middle stir speed (S.S.) is 200rpm~400rpm；Step（6）Middle stir speed (S.S.) is
300rpm~500rpm.
Anaerobic bacteria aerobic bacteria coexists, and can remove suspended solid (SS in water removal simultaneously）, COD (COD）, it is biochemical aerobic
Amount（BOD）, total organic carbon（TOC）, total nitrogen（TN）, total phosphorus（TP）Deng；
Strain is tamed and is strengthened, its digestion power is made to greatly enhance, reaches the ability for handling this sewage；In biosystem
These enzymes for depending in biosystem of reaction accelerate, and strain will produce oxidoreducing enzyme, hydrolase in contaminating action object,
The enzymes such as deamination, decarboxylation, phosphorylation and dephosphorylation.Therefore this process addition sodium gluconate, glycerin monostearate, N, N-
Dimethylacetylamide can not only accelerate the growth of strain, and established in growth forming process as enzyme-producing agent
Rise stronger enzymatic productivity and digestion power, promote waste water treatment system in microorganism metabolism, promote microorganism compared with
It is grown to rapid, high volume in difference ring border, forms good zoogloea, to improve the efficiency of microbial degradation organic pollution, changed
Kind water treatment effect.Multiple-microorganism can also be kept more effective by extending food chain and improving the cycle efficieny of food chain
Ground collaboration plays a role, more thoroughly degradation of contaminant, and improves system resistance to overload shock ability.Add sodium gluconate, list
Tristerin, n,N-dimethylacetamide can improve the vigor of ectoenzyme, improve the permeability of cell, make more enzymes from
Enter in zymotic fluid through cell membrane into the cell, is conducive to the feedback regulation for breaking desmoenzyme synthesis in this way, improves zymotic fluid
The yield of middle enzyme.And the component that the present invention uses is environment-protecting and non-poisonous, will not be generated to strain and poison and will not be caused to environment
Secondary pollution, and certain nutrition and energy can be provided to strain.
Flocculated bacteria can generate microbial flocculant, can not only improve settlement of sewage efficiency, but also without adding chemical flocculation
Agent avoids introducing secondary pollution, and the present invention selects pseudomonas mendocina, Paecilomyces lilacinus, these strains not only to have wadding
Solidifying effect can be with decomposing pollutant, especially protein, fat, the strain to animal oil also, and itself will not be to water body, life
Object causes damages, and the effect being co-administered according to a certain percentage is more preferable than the effect of single administration, due to the whip of actinomyces
Staple length, coiling, therefore pseudomonad and sporangiocyst bacterium can fall on mycelia, the hollow hypha body of hole is formed with, with pollutant
Contact area bigger；Strain selected by the present invention can also resist certain viruses and bacterium, play certain disinfection.
Anaerobe fermentation will produce the fuel gas such as methane, can carry out the utilization of resources by recycling；
Being adsorbed in matrix for microbial inoculum can form biomembrane, with the growth and increment of microorganism, micro organism quantity, type and life
Object film thickness increases, and can form the protozoic food chain of organic pollution-microorganism-, promotes the degradation of organic matter；Profit
Use the nylon cloth of cross, trilobal, five-pointed star weave that there is larger friction coefficient, therefore fabric harsh, thickness as filter membrane
Real, matte can keep plentiful, erect, and have preferable mechanical bulkiness, and be used for a long time it will not hook silk and jump silk and
Broken hole service life is long.Its groove is highly resistant to horizontal direction（Pass in and out flow）And vertical direction（Aeration generates）Flow punching
Brush makes strain be easy to parasitic, breeding at film forming initial stage,；The corner angle side of fiber has better dissection to bubble, improves molten
Coefficient of oxygen utilization is solved, reduces and exposes oxygen power consumption.
This sewage disposal device and sewage treatment process can reduce ventilatory capacity, amount of agitation, drainage flow amount etc., reduce
Foul smell and excess sludge discharge, realize energy-saving and emission-reduction.
Specific implementation mode
Embodiment 1：
（1）The culture of anaerobic bacteria, aerobic bacteria：The preparation of culture medium：Lactose 0.5%, sodium hydrogen selenite 0.8%, disodium hydrogen phosphate
0.5%, l-cysteine 0.4%, peptone 1%, potassium dihydrogen phosphate 0.7%, remaining be water, adjust pH value be 7, by 120 DEG C of high temperature
Sterilize 21min, obtains culture medium A, B, C；1 is pressed in desinfection chamber：1：2 inoculation methane phase bacillus, methane phase coccus, Bacillus acidi lactici are extremely
Culture medium A is 1.5 in proportion in desinfection chamber：0.5：2：3：5 are inoculated with grate sulfur thiobacillus, Thiobacillus ferrooxidans, Qiong Shi not lever
Bacterium, starch Clostridium, saccharomycete to culture medium B, 30 DEG C of cultivation temperature, incubation time 45 hours；
（2）Strain domestication, hot housing：It is put into respectively from secondary sedimentation tank of sewage treatment work in aerobic strain pond and anaerobic species pond
Sludge 30%, sodium hydrogen selenite 0.5%, lactose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 0.5%, the gluconic acid of acquirement
Sodium 2%, glycerin monostearate 1%, n,N-dimethylacetamide 2%, waste water, then add cultured aerobic strain respectively and detest
Oxygen strain；The waste water of pond body product 40%, stirring, stir speed (S.S.) 450rpm are first put into strain pond；Pond body product 6% is added daily
Waste water, until reaching ordinary water level, tamed, strengthen after aerobic and anaerobic sludge, and launched together into anaerobic reaction
Chi Zhong；
（3）The culture of flocculated bacteria：It is inoculated with the strain of flocculation ability in desinfection chamber：Pseudomonas mendocina, Paecilomyces lilacinus,
Inoculative proportion is 7：3 to culture medium C, 26 DEG C of cultivation temperature, incubation time 50 hours；Expand hot housing again, expands and strengthen training
Foster based component is：Sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride 0.5%, glucose
Sour sodium 2%, glycerin monostearate 1%, n,N-dimethylacetamide 1%, it is 7 to adjust pH value；Shaking table is opened, shaking table rate is
100r/min, cultivation temperature are 30 DEG C, incubation time 42h；The strain cultures elder generation high speed centrifugation after hot housing is will be enlarged by,
It is flocculation microbial inoculum to obtain supernatant；
（4）Sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity；It enters back into and overregulates pond adjusting pH
It is 6；
（5）Sewage goes successively to flocculation basin, and 3% flocculation microbial inoculum of sewage volume, stirring, stir speed (S.S.) 300rpm is added；Waterpower is stopped
7h is stayed to remove oil removal and some of the contaminants；Flocculation basin side wall installs aerator, and middle part is equipped with blender, bottom be with
The opposite gradient of water (flow) direction, to collect and be discharged precipitation；
（6）Flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 8h is stirred, and stir speed (S.S.) 400rpm, good bacteria can disappear
Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, generation during this
Methane recovery to anaerobic reation pool connection methane recovery pond；
（7）Sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter
Cobblestone；Next it is the cross nylon filtering cloth of first layer, it is that special-shaped ceramics grow 3~5cm to place filtrate, and mix bacterium agent is being filtered
Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering；The second layer is trilobal weave Buddhist nun
Imperial filter cloth filtrate be special-shaped ceramics grow 1~3cm, third layer be pentagon weave nylon filtering cloth, activated carbon, 5~100 mesh, until under
Up from thick to thin；Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of activated carbon, by
It is arrived carefully to be thick up to lower, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally
It is discharged after detection, anaerobic reation pool is returned if not up to standard.
Embodiment 2：
（1）The culture of anaerobic bacteria, aerobic bacteria：The preparation of culture medium：Lactose 0.5%, sodium hydrogen selenite 0.4%, disodium hydrogen phosphate
0.3%, l-cysteine 0.5%, peptone 1%, potassium dihydrogen phosphate 0.8%, remaining be water, adjust pH value be 7, by 125 DEG C of high temperature
Sterilize 23min, obtains culture medium A, B, C；1 is pressed in desinfection chamber：1：2 inoculation methane phase bacillus, methane phase coccus, Bacillus acidi lactici are extremely
Culture medium A presses 1 in desinfection chamber：1：2：3：5 inoculation Thiobacillus thioxidans, thiobacillus denitrificans, Denitrifying Phosphate Accumulating Organisms, succinic acid are quasi-
Bacillus, saccharomycete to culture medium B, 28 DEG C of cultivation temperature, incubation time 35 hours；
（2）Strain domestication, hot housing：It is put into respectively from secondary sedimentation tank of sewage treatment work in aerobic strain pond and anaerobic species pond
Sludge 20%, sodium hydrogen selenite 0.5%, lactose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 1%, the sodium gluconate of acquirement
1%, glycerin monostearate 2%, n,N-dimethylacetamide 1%, waste water, then cultured aerobic strain and anaerobism are added respectively
Strain；The waste water of pond body product 42%, stirring, stir speed (S.S.) 550rpm are first put into strain pond；Pond body product 7% is added daily
Waste water, until reaching ordinary water level, tamed, strengthen after aerobic and anaerobic sludge, and launched together into anaerobic reation pool
In；
（3）The culture of flocculated bacteria：It is inoculated with the strain of flocculation ability in desinfection chamber：Pseudomonas mendocina, Paecilomyces lilacinus,
Inoculative proportion is 7：3 to culture medium C, 28 DEG C of cultivation temperature, incubation time 47 hours；Expand hot housing again, expands and strengthen training
Foster based component is：Sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride 0.5%, glucose
Sour sodium 1%, glycerin monostearate 2%, n,N-dimethylacetamide 2%, it is 7.5 to adjust pH value；Shaking table is opened, shaking table rate is
150r/min, cultivation temperature are 28 DEG C, incubation time 48h；The strain cultures elder generation high speed centrifugation after hot housing is will be enlarged by,
It is flocculation microbial inoculum to obtain supernatant；
（4）Sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity；It enters back into and overregulates pond adjusting pH
It is 7；
（5）Sewage goes successively to flocculation basin, and 4% flocculation microbial inoculum of sewage volume, stirring, stir speed (S.S.) 350rpm is added；Waterpower is stopped
8h is stayed to remove oil removal and some of the contaminants；Flocculation basin side wall installs aerator, and middle part is equipped with blender, bottom be with
The opposite gradient of water (flow) direction, to collect and be discharged precipitation；
（6）Flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 9h is stirred, and stir speed (S.S.) 450rpm, good bacteria can disappear
Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, generation during this
Methane recovery to anaerobic reation pool connection methane recovery pond；
（7）Sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter
Cobblestone；Next it is the cross nylon filtering cloth of first layer, it is that special-shaped ceramics grow 3~5cm to place filtrate, and mix bacterium agent is being filtered
Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering；The second layer is trilobal weave Buddhist nun
Imperial filter cloth filtrate be special-shaped ceramics grow 1~3cm, third layer be pentagon weave nylon filtering cloth, activated carbon, 5~100 mesh, until under
Up from thick to thin；Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of activated carbon, by
It is arrived carefully to be thick up to lower, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally
It is discharged after detection, anaerobic reation pool is returned if not up to standard.
Embodiment 3：
（1）The culture of anaerobic bacteria, aerobic bacteria：The preparation of culture medium：Lactose 0.4%, sodium hydrogen selenite 0.6%, disodium hydrogen phosphate
0.7%, l-cysteine 0.3%, peptone 1%, potassium dihydrogen phosphate 0.4%, remaining be water, adjust pH value be 7.5, by 115 DEG C of height
Temperature sterilizing 25min, obtains culture medium A, B, C；1 is pressed in desinfection chamber：1：2 inoculation methane phase bacillus, methane phase coccus, Bacillus acidi lactici
It is 2 in proportion in desinfection chamber to culture medium A：2：3：5 inoculation thiobacillus denitrificans, deep red red ring bacterium, Bacillus cercus, yeast
Bacterium is to culture medium B, 25 DEG C of cultivation temperature, incubation time 40 hours；
（2）Strain domestication, hot housing：It is put into respectively from secondary sedimentation tank of sewage treatment work in aerobic strain pond and anaerobic species pond
Sludge 28%, sodium hydrogen selenite 0.5%, lactose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 1%, the sodium gluconate of acquirement
2%, glycerin monostearate 1%, n,N-dimethylacetamide 1%, waste water, then cultured aerobic strain and anaerobism are added respectively
Strain；The waste water of pond body product 45%, stirring, stir speed (S.S.) 500rpm are first put into strain pond；Pond body product 8% is added daily
Waste water, until reaching ordinary water level, tamed, strengthen after aerobic and anaerobic sludge, and launched together into anaerobic reation pool
In；
（3）The culture of flocculated bacteria：It is inoculated with the strain of flocculation ability in desinfection chamber：Pseudomonas mendocina, Paecilomyces lilacinus,
Inoculative proportion is 7：3 to culture medium C, 30 DEG C of cultivation temperature, incubation time 36 hours；Expand hot housing again, expands and strengthen training
Foster based component is：Sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride 0.5%, glucose
Sour sodium 2%, glycerin monostearate 1%, n,N-dimethylacetamide 2%, it is 7.5 to adjust pH value；Shaking table is opened, shaking table rate is
120r/min, cultivation temperature are 26 DEG C, incubation time 41h；The strain cultures elder generation high speed centrifugation after hot housing is will be enlarged by,
It is flocculation microbial inoculum to obtain supernatant；
（4）Sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity；It enters back into and overregulates pond adjusting pH
It is 7.5；
（5）Sewage goes successively to flocculation basin, and 2% flocculation microbial inoculum of sewage volume, stirring, stir speed (S.S.) 250rpm is added；Waterpower is stopped
9h is stayed to remove oil removal and some of the contaminants；Flocculation basin side wall installs aerator, and middle part is equipped with blender, bottom be with
The opposite gradient of water (flow) direction, to collect and be discharged precipitation；
（6）Flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 9.5h, stirring, stir speed (S.S.) 350rpm, good bacteria can
Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, production during this
Raw methane recovery to anaerobic reation pool connects methane recovery pond；
（7）Sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter
Cobblestone；Next it is the cross nylon filtering cloth of first layer, it is that special-shaped ceramics grow 3~5cm to place filtrate, and mix bacterium agent is being filtered
Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering；The second layer is trilobal weave Buddhist nun
Imperial filter cloth filtrate be special-shaped ceramics grow 1~3cm, third layer be pentagon weave nylon filtering cloth, activated carbon, 5~100 mesh, until under
Up from thick to thin；Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of activated carbon, by
It is arrived carefully to be thick up to lower, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally
It is discharged after detection, anaerobic reation pool is returned if not up to standard.
Embodiment 4：
（1）The culture of anaerobic bacteria, aerobic bacteria：The preparation of culture medium：Lactose 0.5%, sodium hydrogen selenite 0.4%, disodium hydrogen phosphate
0.6%, l-cysteine 0.3%, peptone 1%, potassium dihydrogen phosphate 0.7%, remaining be water, adjust pH value be 7, by 110 DEG C of high temperature
Sterilize 24min, obtains culture medium A, B, C；1 is pressed in desinfection chamber：1：2 inoculation methane phase bacillus, methane phase coccus, Bacillus acidi lactici are extremely
Culture medium A is 2 in proportion in desinfection chamber：2：1：2：5 inoculation Thiobacillus thioxidans, Denitrifying Phosphate Accumulating Organisms, starch Clostridium,
Succinic acid bacteroid, saccharomycete to culture medium B, 32 DEG C of cultivation temperature, incubation time 50 hours；
（2）Strain domestication, hot housing：It is put into respectively from secondary sedimentation tank of sewage treatment work in aerobic strain pond and anaerobic species pond
Sludge 25%, sodium hydrogen selenite 0.5%, lactose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 0.5%, the gluconic acid of acquirement
Sodium 2%, glycerin monostearate 1%, n,N-dimethylacetamide 2%, waste water, then add cultured aerobic strain respectively and detest
Oxygen strain；The waste water of pond body product 44%, stirring, stir speed (S.S.) 600rpm are first put into strain pond；Pond body product 7% is added daily
Waste water, until reaching ordinary water level, tamed, strengthen after aerobic and anaerobic sludge, and launched together into anaerobic reaction
Chi Zhong；
（3）The culture of flocculated bacteria：It is inoculated with the strain of flocculation ability in desinfection chamber：Pseudomonas mendocina, Paecilomyces lilacinus,
Inoculative proportion is 7：3 to culture medium C, 31 DEG C of cultivation temperature, incubation time 29 hours；Expand hot housing again, expands and strengthen training
Foster based component is：Sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride 0.5%, glucose
Sour sodium 1%, glycerin monostearate 1%, n,N-dimethylacetamide 2%, it is 7 to adjust pH value；Shaking table is opened, shaking table rate is
120r/min, cultivation temperature are 32 DEG C, incubation time 45h；The strain cultures elder generation high speed centrifugation after hot housing is will be enlarged by,
It is flocculation microbial inoculum to obtain supernatant；
（4）Sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity；It enters back into and overregulates pond adjusting pH
It is 7；
（5）Sewage goes successively to flocculation basin, and 5% flocculation microbial inoculum of sewage volume, stirring, stir speed (S.S.) 200rpm is added；Waterpower is stopped
8.5h is stayed to remove oil removal and some of the contaminants；Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is
The gradient opposite with water (flow) direction, to collect and be discharged precipitation；
（6）Flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 8.5h, stirring, stir speed (S.S.) 300rpm, good bacteria can
Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, production during this
Raw methane recovery to anaerobic reation pool connects methane recovery pond；
（7）Sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter
Cobblestone；Next it is the cross nylon filtering cloth of first layer, it is that special-shaped ceramics grow 3~5cm to place filtrate, and mix bacterium agent is being filtered
Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering；The second layer is trilobal weave Buddhist nun
Imperial filter cloth filtrate be special-shaped ceramics grow 1~3cm, third layer be pentagon weave nylon filtering cloth, activated carbon, 5~100 mesh, until under
Up from thick to thin；Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of activated carbon, by
It is arrived carefully to be thick up to lower, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally
It is discharged after detection, anaerobic reation pool is returned if not up to standard.
Embodiment 5：
（1）The culture of anaerobic bacteria, aerobic bacteria：The preparation of culture medium：Lactose 0.3%, sodium hydrogen selenite 0.8%, disodium hydrogen phosphate
0.5%, l-cysteine 0.6%, peptone 1%, potassium dihydrogen phosphate 0.4%, remaining be water, adjust pH value be 7, by 130 DEG C of high temperature
Sterilize 20min, obtains culture medium A, B, C；1 is pressed in desinfection chamber：1：2 inoculation methane phase bacillus, methane phase coccus, Bacillus acidi lactici are extremely
Culture medium A is 1 in proportion in desinfection chamber：1：2：3：5 inoculation sulfide-oxidizing enzymes are Thiobacillus ferrooxidans, thiobacillus denitrificans, acetic acid
Calcium acinetobacter calcoaceticus, Bacillus cercus, saccharomycete, in culture medium B, 24 DEG C of cultivation temperature, incubation time 25 hours；
（2）Strain domestication, hot housing：It is put into respectively from secondary sedimentation tank of sewage treatment work in aerobic strain pond and anaerobic species pond
Sludge 22%, sodium hydrogen selenite 0.5%, lactose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 1%, the sodium gluconate of acquirement
1%, glycerin monostearate 2%, n,N-dimethylacetamide 2%, waste water, then cultured aerobic strain and anaerobism are added respectively
Strain；The waste water of pond body product 43%, stirring, stir speed (S.S.) 400rpm are first put into strain pond；Pond body product 8% is added daily
Waste water, until reaching ordinary water level, tamed, strengthen after aerobic and anaerobic sludge, and launched together into anaerobic reation pool
In；
（3）The culture of flocculated bacteria：It is inoculated with the strain of flocculation ability in desinfection chamber：Pseudomonas mendocina, Paecilomyces lilacinus,
Inoculative proportion is 7：3 to culture medium C, 27 DEG C of cultivation temperature, incubation time 40 hours；Expand hot housing again, expands and strengthen training
Foster based component is：Sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride 0.5%, glucose
Sour sodium 2%, glycerin monostearate 2%, n,N-dimethylacetamide 2%, it is 7 to adjust pH value；Shaking table is opened, shaking table rate is
110r/min, cultivation temperature are 27 DEG C, incubation time 50h；The strain cultures elder generation high speed centrifugation after hot housing is will be enlarged by,
It is flocculation microbial inoculum to obtain supernatant；
（4）Sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity；It enters back into and overregulates pond adjusting pH
It is 6；
（5）Sewage goes successively to flocculation basin, and 3% flocculation microbial inoculum of sewage volume, stirring, stir speed (S.S.) 400rpm is added；Waterpower is stopped
8.5h is stayed to remove oil removal and some of the contaminants；Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is
The gradient opposite with water (flow) direction, to collect and be discharged precipitation；
（6）Flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 10h, stirring, stir speed (S.S.) 500rpm, good bacteria can
Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, production during this
Raw methane recovery to anaerobic reation pool connects methane recovery pond；
（7）Sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter
Cobblestone；Next it is the cross nylon filtering cloth of first layer, it is that special-shaped ceramics grow 3~5cm to place filtrate, and mix bacterium agent is being filtered
Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering；The second layer is trilobal weave Buddhist nun
Imperial filter cloth filtrate be special-shaped ceramics grow 1~3cm, third layer be pentagon weave nylon filtering cloth, activated carbon, 5~100 mesh, until under
Up from thick to thin；Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of activated carbon, by
It is arrived carefully to be thick up to lower, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally
It is discharged after detection, anaerobic reation pool is returned if not up to standard.
Index before and after 1. each instance processes of table
Claims (9)
1. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage, which is characterized in that include the following steps：
（1）The culture of anaerobic bacteria, aerobic bacteria：The preparation of culture medium：Lactose 0.3~0.5%, sodium hydrogen selenite 0.3~0.8%, phosphorus
Sour disodium hydrogen 0.3~0.8%, l-cysteine 0.3~0.5%, peptone 1%, potassium dihydrogen phosphate 0.3~0.8%, remaining be water, adjust
It is 7.0~7.5 to save pH value, by 110~130 DEG C of 20~25min of high-temperature sterilization, obtains culture medium A, B, C；One is pressed in desinfection chamber
Certainty ratio is inoculated with anaerobic species to culture medium A, is inoculated with by a certain percentage in aerobic bacteria to culture medium B, cultivation temperature in desinfection chamber
24~32 DEG C, incubation time 24~50 hours；
（2）Strain domestication, hot housing：It is put into respectively from secondary sedimentation tank of sewage treatment work in aerobic strain pond and anaerobic species pond
The sludge 20~30% of acquirement, sodium hydrogen selenite 0.5%, lactose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 0.5~1%, Portugal
Grape sodium saccharate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2%, waste water, then add cultivate respectively
Aerobic strain and anaerobic species；The waste water of pond body product 40~45%, stirring are first put into strain pond；Pond body product 6 is added daily
~8% waste water, until reaching ordinary water level, tamed, strengthen after aerobic and anaerobic sludge, and launched together into anaerobism
In reaction tank；
（3）The culture of flocculated bacteria：It is inoculated with the strain of flocculation ability in desinfection chamber：Pseudomonas mendocina, Paecilomyces lilacinus,
Inoculative proportion is 7：3 to culture medium C, 24~32 DEG C of cultivation temperature, incubation time 24~50 hours；Expand hot housing again, expands
Big hot housing based component is：Sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride
0.5%, sodium gluconate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2%, it is 7.0 to adjust pH value
~7.5；Shaking table is opened, cultivation temperature is 30 DEG C, incubation time 48h；It is first high to will be enlarged by the strain cultures after hot housing
Speed centrifugation, it is flocculation microbial inoculum to obtain supernatant；
（4）Sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity；It enters back into and overregulates pond adjusting pH
It is 6~7.5；
（5）Sewage goes successively to flocculation basin, and 2~5% flocculation microbial inoculum of sewage volume, stirring is added, and hydraulic retention 7~9h removals are hanged
Float and some of the contaminants；Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is and water (flow) direction phase
The anti-gradient, to collect and be discharged precipitation；
（6）Flocculation basin supernatant enters anaerobic reation pool, and 8~10h of hydraulic retention is stirred, and good bacteria can consume extra oxygen point
Pollutant is solved, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, the methane recovery generated during this is extremely
Anaerobic reation pool connects methane recovery pond；
（7）Sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter
Cobblestone；Next it is the cross nylon filtering cloth of first layer, it is that special-shaped ceramics grow 3~5cm to place filtrate, and mix bacterium agent is being filtered
Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering；The second layer is trilobal weave Buddhist nun
Imperial filter cloth filtrate be special-shaped ceramics grow 1~3cm, third layer be pentagon weave nylon filtering cloth, activated carbon, 5~100 mesh, until under
Up from thick to thin；Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of activated carbon, by
It is arrived carefully to be thick up to lower, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally
It is discharged after detection, anaerobic reation pool is returned if not up to standard；
Sewage disposal device includes：It is aerobic seeding tank, anaerobic species tank, grid, regulating reservoir, flocculation basin, anaerobic reation pool, aerobic
Biological aerated filter, methane recovery device.
2. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 1, it is characterised in that：Anaerobic species are
The combination of methane phase bacillus, methane phase coccus, Bacillus acidi lactici.
3. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 2, it is characterised in that：Methane phase bar
Bacterium, methane phase coccus, Bacillus acidi lactici inoculative proportion be 1：1：2.
4. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 1, it is characterised in that：Aerobic strain is
The combination of sulfide-oxidizing enzyme, polyP bacteria, hydrolysis acidification bacterium, saccharomycete.
5. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 4, it is characterised in that：Sulfide-oxidizing enzyme,
PolyP bacteria, hydrolysis acidification bacterium, saccharomycete inoculative proportion be 2：2：3：5.
6. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 4, it is characterised in that：Sulfide-oxidizing enzyme is
One or both of Thiobacillus thioxidans, grate sulfur thiobacillus, Thiobacillus ferrooxidans, thiobacillus denitrificans.
7. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 4, it is characterised in that：Calcium acetate is motionless
One or both of bacillus, Acinetobacter junii, Denitrifying Phosphate Accumulating Organisms, deep red red ring bacterium.
8. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 4, it is characterised in that：Hydrolysis acidification bacterium
For one or both of Bacillus cercus, starch Clostridium, succinic acid bacteroid.
9. a kind of microbial inoculum treatment process of livestock and poultry cultivation sewage according to claim 1, it is characterised in that：Step（2）In
Stir speed (S.S.) be 400rpm~600rpm；Step（3）Middle shaking table rate is 100~150r/min；Step（5）Middle stir speed (S.S.)
For 200rpm~400rpm；Step（6）Middle stir speed (S.S.) is 300rpm~500rpm.
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| CN110803826A (en) * | 2019-10-15 | 2020-02-18 | 广州市凯卫莎环保科技有限公司 | System for treating sewage by adopting microorganisms and treatment method thereof |
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