CN109052822A - A kind of microbial inoculum treatment process of town sewage - Google Patents

A kind of microbial inoculum treatment process of town sewage Download PDF

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Publication number
CN109052822A
CN109052822A CN201810937602.8A CN201810937602A CN109052822A CN 109052822 A CN109052822 A CN 109052822A CN 201810937602 A CN201810937602 A CN 201810937602A CN 109052822 A CN109052822 A CN 109052822A
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anaerobic
sewage
aerobic
bacteria
microbial inoculum
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黄陈玉
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Nanning Huang Chen Pig Farm
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Nanning Huang Chen Pig Farm
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00Multistage treatment of water, waste water or sewage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/001Processes for the treatment of water whereby the filtration technique is of importance
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/30Treatment of water, waste water, or sewage by irradiation
    • C02F1/32Treatment of water, waste water, or sewage by irradiation with ultraviolet light
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/66Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2301/00General aspects of water treatment
    • C02F2301/08Multistage treatments, e.g. repetition of the same process step under different conditions
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00Specific treatment goals
    • C02F2303/04Disinfection
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/28Anaerobic digestion processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/30Aerobic and anaerobic processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/30Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

The invention discloses a kind of microbial inoculum treatment process of town sewage, device includes: aerobic seeding tank, anaerobic species tank, grid, conditioning tank, flocculation basin, anaerobic reation pool, aerobic aeration biofilter, methane recovery device.Operating procedure includes: the configuration of culture medium;The culture of anaerobic bacteria;The culture of aerobic bacteria;The culture of flocculated bacteria;Strain expands, and strengthens, domestication culture;Sewage adjusts pH through grid, in conditioning tank;Into flocculation basin, flocculation microbial inoculum is added;Sewage enters anaerobic reation pool and stops 8~10h, and methane is recycled in stirring;Sewage flows into aerobic aeration biofilter, by three layers of profiled filament nylon filtering cloth;Gravity secondary filter, ultraviolet lamp disinfection discharge after detection, then return to anaerobic reation pool if it does not meet the standards.Good sewage processing effect, no added chemical reagent avoid introducing secondary pollution;Ventilatory capacity, amount of agitation, drainage flow amount etc. are low, and foul smell and excess sludge discharge are few, realize energy-saving and emission-reduction.

Description

A kind of microbial inoculum treatment process of town sewage
Technical field
The present invention relates to the microbial inoculum treatment process of sewage treatment process, in particular to a kind of town sewage.
Background technique
Trade effluent or municipal wastewater major pollutants are that oxygen consumption pollutant is (carbohydrate, protein, grease, wooden The organic substances such as element), toxic pollutant (inorganic anion, organic agricultural chemicals etc.), inorganic pollution (acid, alkali, salt etc.) and cause of disease Body pollution object.Traditional sewage water treatment method is stirring, aerating oxidation processing, without gas Anaerobic Treatment and precipitating, oxidation Processing and Anaerobic Treatment are substantially exactly the process of microbial degradation water pollutant, are usually all natural strain or manually add Add a small amount of strain, also needs to add part chemical reagent according to pollutant kind, precipitation process mostly uses nature to stand or add Flocculant precipitating.The inefficiency of strain processing sewage without screening and optimizing needs fiting chemical reagent or physical method, increases The possibility for having added secondary pollution also improves time and the economic cost of waste water control.In addition, sewage disposal device is for sewage Treatment effect no less important, most sewage disposal device capacity is small, running efficiency is low, pollutant process is not thorough, easily produces The foul smell and sludge of raw secondary pollution.Therefore, the dominant bacteria of sewage treatment is screened, matched advanced sewage treatment is developed and sets It is standby, it will help the ability and efficiency for improving existing sewage treatment solve the problems, such as sewage treatment.
A kind of Chinese patent (CN201810012965.0) wastewater treatment equipment uses anaerobic species and facultative aerobe kind or good The flocculation that the mixture of oxygen strain can achieve good wastewater treatment efficiency, but use as microbial water-purifying agent processing sewage Agent is inorganic flocculating agent and organic polymer coargulator, may cause secondary pollution to environment.Chinese patent (CN201611182630.0) a kind of technique for treating industrial wastewater handles waste water using mix bacterium agent, but due to not carrying out to strain The ability of domestication, strain processing waste water lowly easily can make the waste water given off that processing standard be not achieved.
Summary of the invention
In view of the deficiencies of the prior art, a kind of microbial inoculum treatment process of town sewage, wastewater treatment efficiency were provided Good, no added chemical reagent avoids introducing secondary pollution;The recycling of the gases such as methane carries out the utilization of resources;Ventilatory capacity, amount of agitation, Drainage flow amount etc. is low, and foul smell and excess sludge discharge are few, realizes energy-saving and emission-reduction.
Technical scheme is as follows:
(1) culture of anaerobic bacteria, aerobic bacteria: the preparation of culture medium: agar 1%, stalk powder 0.5%, peptone 1%, magnesium sulfate 0.3~0.8%, glucose 0.3~0.5%, potassium dihydrogen phosphate 0.3~0.8%, remaining be water, adjust pH value be 7.0~7.5, warp 110~130 DEG C of 20~25min of high-temperature sterilization are crossed, culture medium A, B, C are obtained;It is inoculated with anaerobic species by a certain percentage in desinfection chamber To culture medium A, it is inoculated in aerobic bacteria by a certain percentage in desinfection chamber to culture medium B, 24~32 DEG C of cultivation temperature, incubation time 24 ~50 hours;
(2) it strain domestication, hot housing: is put into respectively in aerobic strain pond and anaerobic species pond from secondary sedimentation tank of sewage treatment work Sludge 20~30%, magnesium sulfate 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.5%, the multi-vitamins 0.5~1%, Portugal of acquirement Grape sodium saccharate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2%, waste water, then add cultivate respectively Aerobic strain and anaerobic species;The waste water of pond body product 40~45%, stirring are first put into strain pond;Pond body product 6 is added daily ~8% waste water, until reaching ordinary water level, aerobic and anaerobic sludge after being tamed, being strengthened, and launched together into anaerobism In reaction tank;
(3) it the culture of flocculated bacteria: is inoculated with the strain of flocculation ability: rose pink mold cyst bacterium, Fei Lv by a certain percentage in desinfection chamber Guest's actinoplanes, inoculative proportion are 1:1 to culture medium C, 24~32 DEG C of cultivation temperature, incubation time 24~50 hours;Expand again Big hot housing expands hot housing based component are as follows: sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride 0.5%, sodium gluconate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2% are adjusted Saving pH value is 7.0~7.5;Shaking table is opened, cultivation temperature is 25~32 DEG C, and incubation time is 40~50h;It will be enlarged by hot housing Strain cultures elder generation high speed centrifugation afterwards obtains supernatant as flocculation microbial inoculum;
(4) sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity;It enters back into and overregulates pond adjusting pH It is 6~7.5;
(5) sewage goes successively to flocculation basin, and the flocculation microbial inoculum of sewage volume 2~5%, stirring is added, and 7~9h of hydraulic retention removal is hanged Floating object and some of the contaminants;Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is and water (flow) direction phase The anti-gradient, to collect and be discharged precipitating;
(6) flocculation basin supernatant enters anaerobic reation pool, and 8~10h of hydraulic retention is stirred, and good bacteria can consume extra oxygen point Pollutant is solved, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, the methane recovery generated during this is extremely Anaerobic reation pool connects methane recovery pond;
(7) sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter Cobblestone;It next is the cross nylon filtering cloth of first layer, placing filtrate is the long 3~5cm of special-shaped ceramics, and mix bacterium agent is being filtered Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering;The second layer is trilobal weave Buddhist nun Imperial filter cloth filtrate is the long 1~3cm of special-shaped ceramics, and third layer is pentagon weave nylon filtering cloth, active carbon, 5~100 mesh, until under Up from thick to thin;Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of active carbon, by It is arrived carefully up to lower to be thick, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally It is discharged after detection, then returns to anaerobic reation pool if it does not meet the standards.
Sewage disposal device include: aerobic seeding tank, anaerobic species tank, grid, conditioning tank, flocculation basin, anaerobic reation pool, Aerobic aeration biofilter, methane recovery device.
As a further explanation of the present invention, anaerobic species be methane phase bacillus, methane phase coccus, Bacillus acidi lactici group It closes.
As a further explanation of the present invention, methane phase bacillus, methane phase coccus, Bacillus acidi lactici inoculative proportion are 2:2:3.
As a further explanation of the present invention, aerobic strain is polyP bacteria, hydrolysis acidification bacterium, three kinds or more in saccharomycete Combination.
As a further explanation of the present invention, polyP bacteria, hydrolysis acidification bacterium, saccharomycete inoculative proportion be 3:2:5.
As a further explanation of the present invention, polyP bacteria is Acinetobacter calcoaceticus, Acinetobacter junii, denitrification phosphorus-collecting One or both of bacterium, deep red red ring bacterium.
As a further explanation of the present invention, hydrolysis acidification bacterium is Bacillus cercus, starch Clostridium, succinic acid are intended One or both of bacillus.
As a further explanation of the present invention, the stirring rate in step (2) is 400rpm~600rpm;In step (3) Shaking table rate is 100~150r/min;Stirring rate is 200rpm~400rpm in step (5);Stirring rate is in step (6) 300rpm~500rpm.
Anaerobic bacteria aerobic bacteria coexists, and can go suspended solid (SS) in water removal, COD (COD), biochemistry aerobic simultaneously Measure (BOD), total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP) etc.;
Strain is tamed and is strengthened, its digestion power is greatly enhanced, reaches the ability for handling this sewage;In biosystem These enzymes for depending in biosystem of reaction accelerate, and strain can generate oxidoreducing enzyme, hydrolase in contaminating action object, The enzymes such as deamination, decarboxylation, phosphorylation and dephosphorylation.Therefore this process adds sodium gluconate, glycerin monostearate, N, N- Dimethyl acetamide can not only accelerate the growth of strain as enzyme-producing agent, and establish during its growth shaping Rise stronger enzymatic productivity and digestion power, promote waste water treatment system in microorganism metabolism, promote microorganism compared with It is grown to rapid, high volume in difference ring border, forms good zoogloea, to improve the efficiency of microbial degradation organic pollutant, changed Kind water treatment effect.Multiple-microorganism can also be kept more effective by extending food chain and improving the cycle efficieny of food chain Ground collaboration plays a role, more thoroughly degradation of contaminant, and improves system resistance to overload shock ability.Add sodium gluconate, list The vigor of ectoenzyme can be improved in tristerin, n,N-dimethylacetamide, improves the permeability of cell, make more enzymes from Enter in fermentation liquid through cell membrane into the cell, is conducive to the feedback regulation for breaking desmoenzyme synthesis in this way, improves fermentation liquid The yield of middle enzyme.And the component that uses of the present invention be it is environment-protecting and non-poisonous, strain will not be generated and poison and environment will not be caused Secondary pollution, and certain nutrition and energy can be provided to strain.
Flocculated bacteria can produce microbial flocculant, settlement of sewage efficiency not only can be improved, but also without adding chemical flocculation Agent avoids introducing secondary pollution, and the present invention selects rose pink mold cyst bacterium, actinoplanes philippinensis, these strains not only have Flocculation can be with the nitrogen of decomposing pollutant, especially town sewage, phosphorus, and itself will not cause to endanger to water body, organism Evil, the effect being co-administered according to a certain percentage is more preferable than the effect of single administration, since the flagellum of actinomyces is long, coils, Therefore pseudomonad and sporangiocyst bacterium can fall on mycelia, be formed with the hollow hypha body of hole, more with the contact area of pollutant Greatly;Strain selected by the present invention can also resist certain viruses and bacterium, play certain disinfection.
Anaerobe fermentation can generate the fuel gas such as methane, can carry out the utilization of resources by recycling;
Being adsorbed in matrix for microbial inoculum will form biomembrane, with the growth and increment of microorganism, micro organism quantity, type and life Object film thickness increases, and can form the protozoic food chain of organic pollutant-microorganism-, promotes the degradation of organic matter;Benefit Using the nylon cloth of cross, trilobal, five-pointed star weave has biggish coefficient of friction, therefore fabric harsh, thickness as filter membrane Real, flannelette can keep plentiful, erect, and have preferable mechanical bulkiness, and be used for a long time it will not hook silk and jump silk and Broken hole long service life.Its groove is highly resistant to the water flow punching of horizontal direction (disengaging water flow) and vertical direction (aeration generates) Brush makes strain be easy to parasitic, breeding at film forming initial stage,;The corner angle side of fiber has better dissection to bubble, improves molten Coefficient of oxygen utilization is solved, reduces and exposes oxygen power consumption.
This sewage disposal device and sewage treatment process can reduce ventilatory capacity, amount of agitation, drainage flow amount etc., reduce Foul smell and excess sludge discharge, realize energy-saving and emission-reduction.
Specific embodiment
Embodiment 1:
(1) culture of anaerobic bacteria, aerobic bacteria: the preparation of culture medium: agar 1%, stalk powder 0.5%, peptone 1%, magnesium sulfate 0.4%, glucose 0.5%, potassium dihydrogen phosphate 0.6%, remaining be water, adjust pH value be 7, by 120 DEG C of high-temperature sterilization 21min, obtain To culture medium A, B, C;It is inoculated with methane phase bacillus, methane phase coccus, Bacillus acidi lactici to culture medium A by 2:2:3 in desinfection chamber, in nothing Bacterium room is inoculated with Acinetobacter junii, starch Clostridium, saccharomycete to culture medium B by 3:2:5, and 30 DEG C of cultivation temperature, when culture Between 45 hours;
(2) it strain domestication, hot housing: is put into respectively in aerobic strain pond and anaerobic species pond from secondary sedimentation tank of sewage treatment work Sludge 30%, magnesium sulfate 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.5%, the multi-vitamins 0.5%, sodium gluconate of acquirement 2%, glycerin monostearate 1%, n,N-dimethylacetamide 2%, waste water, then cultured aerobic strain and anaerobism are added respectively Strain;The waste water of pond body product 40%, stirring, stirring rate 450rpm are first put into strain pond;Pond body product 6% is added daily Waste water, until reaching ordinary water level, aerobic and anaerobic sludge after being tamed, being strengthened, and launched together into anaerobic reation pool In;
(3) it the culture of flocculated bacteria: is inoculated with the strain of flocculation ability: rose pink mold cyst bacterium, Fei Lv by a certain percentage in desinfection chamber Guest's actinoplanes, inoculative proportion are 1:1 to culture medium C, 26 DEG C of cultivation temperature, incubation time 50 hours;Expand reinforcing training again It supports, expands hot housing based component are as follows: sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, chlorination Sodium 0.5%, sodium gluconate 2%, glycerin monostearate 1%, n,N-dimethylacetamide 1%, adjusting pH value is 7;Shaking table is opened, Shaking table rate is 100r/min, and cultivation temperature is 30 DEG C, incubation time 42h;Strain cultures after will be enlarged by hot housing First high speed centrifugation obtains supernatant as flocculation microbial inoculum;
(4) sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity;It enters back into and overregulates pond adjusting pH It is 6;
(5) sewage goes successively to flocculation basin, and the flocculation microbial inoculum of sewage volume 3%, stirring, stirring rate 300rpm is added;Waterpower is stopped 7h is stayed to remove oil removal and some of the contaminants;Flocculation basin side wall install aerator, middle part be equipped with blender, bottom be with The opposite gradient of water (flow) direction, to collect and be discharged precipitating;
(6) flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 8h is stirred, and stirring rate 400rpm, good bacteria can disappear Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, generation during this Methane recovery to anaerobic reation pool connect methane recovery pond;
(7) sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter Cobblestone;It next is the cross nylon filtering cloth of first layer, placing filtrate is the long 3~5cm of special-shaped ceramics, and mix bacterium agent is being filtered Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering;The second layer is trilobal weave Buddhist nun Imperial filter cloth filtrate is the long 1~3cm of special-shaped ceramics, and third layer is pentagon weave nylon filtering cloth, active carbon, 5~100 mesh, until under Up from thick to thin;Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of active carbon, by It is arrived carefully up to lower to be thick, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally It is discharged after detection, then returns to anaerobic reation pool if it does not meet the standards.
Embodiment 2:
(1) culture of anaerobic bacteria, aerobic bacteria: the preparation of culture medium: agar 1%, stalk powder 0.5%, peptone 1%, magnesium sulfate 0.7%, glucose 0.3%, potassium dihydrogen phosphate 0.8%, remaining be water, adjust pH value be 7, by 125 DEG C of high-temperature sterilization 23min, obtain To culture medium A, B, C;It is inoculated with methane phase bacillus, methane phase coccus, Bacillus acidi lactici to culture medium A by 2:2:3 in desinfection chamber, in nothing Bacterium room is inoculated with Acinetobacter calcoaceticus, Acinetobacter junii, succinic acid bacteroid, saccharomycete to culture medium B, training by 1:2:2:5 Feeding 28 DEG C of temperature, incubation time 35 hours;
(2) it strain domestication, hot housing: is put into respectively in aerobic strain pond and anaerobic species pond from secondary sedimentation tank of sewage treatment work The sludge 20% of acquirement, magnesium sulfate 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 1%, sodium gluconate 1%, Glycerin monostearate 2%, n,N-dimethylacetamide 1%, waste water, then cultured aerobic strain and anaerobic bacteria are added respectively Kind;The waste water of pond body product 42%, stirring, stirring rate 550rpm are first put into strain pond;The useless of pond body product 7% is added daily Water, until reaching ordinary water level, aerobic and anaerobic sludge after being tamed, being strengthened, and launch in anaerobic reation pool together;
(3) it the culture of flocculated bacteria: is inoculated with the strain of flocculation ability: rose pink mold cyst bacterium, Fei Lv by a certain percentage in desinfection chamber Guest's actinoplanes, inoculative proportion are 1:1 to culture medium C, 28 DEG C of cultivation temperature, incubation time 47 hours;Expand reinforcing training again It supports, expands hot housing based component are as follows: sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, chlorination Sodium 0.5%, sodium gluconate 1%, glycerin monostearate 2%, n,N-dimethylacetamide 2%, adjusting pH value is 7.5;Unlatching is shaken Bed, shaking table rate are 150r/min, and cultivation temperature is 28 DEG C, incubation time 48h;Strain culturing after will be enlarged by hot housing Base elder generation high speed centrifugation obtains supernatant as flocculation microbial inoculum;
(4) sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity;It enters back into and overregulates pond adjusting pH It is 7;
(5) sewage goes successively to flocculation basin, and the flocculation microbial inoculum of sewage volume 4%, stirring, stirring rate 350rpm is added;Waterpower is stopped 8h is stayed to remove oil removal and some of the contaminants;Flocculation basin side wall install aerator, middle part be equipped with blender, bottom be with The opposite gradient of water (flow) direction, to collect and be discharged precipitating;
(6) flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 9h is stirred, and stirring rate 450rpm, good bacteria can disappear Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, generation during this Methane recovery to anaerobic reation pool connect methane recovery pond;
(7) sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter Cobblestone;It next is the cross nylon filtering cloth of first layer, placing filtrate is the long 3~5cm of special-shaped ceramics, and mix bacterium agent is being filtered Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering;The second layer is trilobal weave Buddhist nun Imperial filter cloth filtrate is the long 1~3cm of special-shaped ceramics, and third layer is pentagon weave nylon filtering cloth, active carbon, 5~100 mesh, until under Up from thick to thin;Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of active carbon, by It is arrived carefully up to lower to be thick, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally It is discharged after detection, then returns to anaerobic reation pool if it does not meet the standards.
Embodiment 3:
(1) culture of anaerobic bacteria, aerobic bacteria: the preparation of culture medium: agar 1%, stalk powder 0.5%, peptone 1%, magnesium sulfate 0.3%, glucose 0.4%, potassium dihydrogen phosphate 0.3%, remaining be water, adjusting pH value is 7.5, by 115 DEG C of high-temperature sterilization 25min, Obtain culture medium A, B, C;It is inoculated with methane phase bacillus, methane phase coccus, Bacillus acidi lactici to culture medium A by 2:2:3 in desinfection chamber, Desinfection chamber by 3:1:1:5 be inoculated with deep red red ring bacterium, Bacillus cercus, succinic acid bacteroid saccharomycete to culture medium B, culture 25 DEG C of temperature, incubation time 40 hours;
(2) it strain domestication, hot housing: is put into respectively in aerobic strain pond and anaerobic species pond from secondary sedimentation tank of sewage treatment work The sludge 28% of acquirement, magnesium sulfate 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 1%, sodium gluconate 2%, Glycerin monostearate 1%, n,N-dimethylacetamide 1%, waste water, then cultured aerobic strain and anaerobic bacteria are added respectively Kind;The waste water of pond body product 45%, stirring, stirring rate 500rpm are first put into strain pond;The useless of pond body product 8% is added daily Water, until reaching ordinary water level, aerobic and anaerobic sludge after being tamed, being strengthened, and launch in anaerobic reation pool together;
(3) it the culture of flocculated bacteria: is inoculated with the strain of flocculation ability: rose pink mold cyst bacterium, Fei Lv by a certain percentage in desinfection chamber Guest's actinoplanes, inoculative proportion are 1:1 to culture medium C, 30 DEG C of cultivation temperature, incubation time 36 hours;Expand reinforcing training again It supports, expands hot housing based component are as follows: sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, chlorination Sodium 0.5%, sodium gluconate 2%, glycerin monostearate 1%, n,N-dimethylacetamide 1%, adjusting pH value is 7.5;Unlatching is shaken Bed, shaking table rate are 120r/min, and cultivation temperature is 26 DEG C, incubation time 41h;Strain culturing after will be enlarged by hot housing Base elder generation high speed centrifugation obtains supernatant as flocculation microbial inoculum;
(4) sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity;It enters back into and overregulates pond adjusting pH It is 7.5;
(5) sewage goes successively to flocculation basin, and the flocculation microbial inoculum of sewage volume 2%, stirring, stirring rate 250rpm is added;Waterpower is stopped 9h is stayed to remove oil removal and some of the contaminants;Flocculation basin side wall install aerator, middle part be equipped with blender, bottom be with The opposite gradient of water (flow) direction, to collect and be discharged precipitating;
(6) flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 9.5h, stirring, stirring rate 350rpm, good bacteria can Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, production during this Raw methane recovery to anaerobic reation pool connects methane recovery pond;
(7) sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter Cobblestone;It next is the cross nylon filtering cloth of first layer, placing filtrate is the long 3~5cm of special-shaped ceramics, and mix bacterium agent is being filtered Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering;The second layer is trilobal weave Buddhist nun Imperial filter cloth filtrate is the long 1~3cm of special-shaped ceramics, and third layer is pentagon weave nylon filtering cloth, active carbon, 5~100 mesh, until under Up from thick to thin;Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of active carbon, by It is arrived carefully up to lower to be thick, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally It is discharged after detection, then returns to anaerobic reation pool if it does not meet the standards.
Embodiment 4:
(1) culture of anaerobic bacteria, aerobic bacteria: the preparation of culture medium: agar 1%, stalk powder 0.5%, peptone 1%, magnesium sulfate 0.5%, glucose 0.4%, potassium dihydrogen phosphate 0.5%, remaining be water, adjust pH value be 7, by 110 DEG C of high-temperature sterilization 24min, obtain To culture medium A, B, C;It is inoculated with methane phase bacillus, methane phase coccus, Bacillus acidi lactici to culture medium A by 2:2:3 in desinfection chamber, in nothing Bacterium room is inoculated with Acinetobacter junii, Denitrifying Phosphate Accumulating Organisms, starch Clostridium, succinic acid bacteroid, saccharomycete by 2:1:1:1:5 To culture medium B, 32 DEG C of cultivation temperature, incubation time 50 hours;
(2) it strain domestication, hot housing: is put into respectively in aerobic strain pond and anaerobic species pond from secondary sedimentation tank of sewage treatment work Sludge 25%, magnesium sulfate 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.5%, the multi-vitamins 0.5%, sodium gluconate of acquirement 2%, glycerin monostearate 1%, n,N-dimethylacetamide 2%, waste water, then cultured aerobic strain and anaerobism are added respectively Strain;The waste water of pond body product 44%, stirring, stirring rate 600rpm are first put into strain pond;Pond body product 7% is added daily Waste water, until reaching ordinary water level, aerobic and anaerobic sludge after being tamed, being strengthened, and launched together into anaerobic reation pool In;
(3) it the culture of flocculated bacteria: is inoculated with the strain of flocculation ability: rose pink mold cyst bacterium, Fei Lv by a certain percentage in desinfection chamber Guest's actinoplanes, inoculative proportion are 1:1 to culture medium C, 31 DEG C of cultivation temperature, incubation time 29 hours;Expand reinforcing training again It supports, expands hot housing based component are as follows: sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, chlorination Sodium 0.5%, sodium gluconate 1%, glycerin monostearate 1%, n,N-dimethylacetamide 2%, adjusting pH value is 7;Shaking table is opened, Shaking table rate is 120r/min, and cultivation temperature is 32 DEG C, incubation time 45h;Strain cultures after will be enlarged by hot housing First high speed centrifugation obtains supernatant as flocculation microbial inoculum;
(4) sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity;It enters back into and overregulates pond adjusting pH It is 7;
(5) sewage goes successively to flocculation basin, and the flocculation microbial inoculum of sewage volume 5%, stirring, stirring rate 200rpm is added;Waterpower is stopped 8.5h is stayed to remove oil removal and some of the contaminants;Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is The gradient opposite with water (flow) direction, to collect and be discharged precipitating;
(6) flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 8.5h, stirring, stirring rate 300rpm, good bacteria can Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, production during this Raw methane recovery to anaerobic reation pool connects methane recovery pond;
(7) sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter Cobblestone;It next is the cross nylon filtering cloth of first layer, placing filtrate is the long 3~5cm of special-shaped ceramics, and mix bacterium agent is being filtered Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering;The second layer is trilobal weave Buddhist nun Imperial filter cloth filtrate is the long 1~3cm of special-shaped ceramics, and third layer is pentagon weave nylon filtering cloth, active carbon, 5~100 mesh, until under Up from thick to thin;Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of active carbon, by It is arrived carefully up to lower to be thick, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally It is discharged after detection, then returns to anaerobic reation pool if it does not meet the standards.
Embodiment 5:
(1) culture of anaerobic bacteria, aerobic bacteria: the preparation of culture medium: agar 1%, stalk powder 0.5%, peptone 1%, magnesium sulfate 0.8%, glucose 0.5%, potassium dihydrogen phosphate 0.7%, remaining be water, adjust pH value be 7, by 130 DEG C of high-temperature sterilization 20min, obtain To culture medium A, B, C;It is inoculated with methane phase bacillus, methane phase coccus, Bacillus acidi lactici to culture medium A by 2:2:3 in desinfection chamber, in nothing Bacterium room is inoculated with Acinetobacter calcoaceticus, Acinetobacter junii, deep red red ring bacterium, succinic acid bacteroid, yeast by 1:1:1:2:5 Bacterium, in culture medium B, 24 DEG C of cultivation temperature, incubation time 25 hours;
(2) it strain domestication, hot housing: is put into respectively in aerobic strain pond and anaerobic species pond from secondary sedimentation tank of sewage treatment work The sludge 22% of acquirement, magnesium sulfate 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.5%, multi-vitamins 1%, sodium gluconate 1%, Glycerin monostearate 2%, n,N-dimethylacetamide 2%, waste water, then cultured aerobic strain and anaerobic bacteria are added respectively Kind;The waste water of pond body product 43%, stirring, stirring rate 400rpm are first put into strain pond;The useless of pond body product 8% is added daily Water, until reaching ordinary water level, aerobic and anaerobic sludge after being tamed, being strengthened, and launch in anaerobic reation pool together;
(3) it the culture of flocculated bacteria: is inoculated with the strain of flocculation ability: rose pink mold cyst bacterium, Fei Lv by a certain percentage in desinfection chamber Guest's actinoplanes, inoculative proportion are 1:1 to culture medium C, 27 DEG C of cultivation temperature, incubation time 40 hours;Expand reinforcing training again It supports, expands hot housing based component are as follows: sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, chlorination Sodium 0.5%, sodium gluconate 2%, glycerin monostearate 2%, n,N-dimethylacetamide 1%, adjusting pH value is 7;Shaking table is opened, Shaking table rate is 110r/min, and cultivation temperature is 27 DEG C, incubation time 50h;Strain cultures after will be enlarged by hot housing First high speed centrifugation obtains supernatant as flocculation microbial inoculum;
(4) sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity;It enters back into and overregulates pond adjusting pH It is 6;
(5) sewage goes successively to flocculation basin, and the flocculation microbial inoculum of sewage volume 3%, stirring, stirring rate 400rpm is added;Waterpower is stopped 8.5h is stayed to remove oil removal and some of the contaminants;Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is The gradient opposite with water (flow) direction, to collect and be discharged precipitating;
(6) flocculation basin supernatant enters anaerobic reation pool, and hydraulic retention 10h, stirring, stirring rate 500rpm, good bacteria can Extra oxygen decomposing pollutant is consumed, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, production during this Raw methane recovery to anaerobic reation pool connects methane recovery pond;
(7) sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter Cobblestone;It next is the cross nylon filtering cloth of first layer, placing filtrate is the long 3~5cm of special-shaped ceramics, and mix bacterium agent is being filtered Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering;The second layer is trilobal weave Buddhist nun Imperial filter cloth filtrate is the long 1~3cm of special-shaped ceramics, and third layer is pentagon weave nylon filtering cloth, active carbon, 5~100 mesh, until under Up from thick to thin;Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of active carbon, by It is arrived carefully up to lower to be thick, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally It is discharged after detection, then returns to anaerobic reation pool if it does not meet the standards.
Index before and after each instance processes of table 1.

Claims (8)

1. a kind of microbial inoculum treatment process of town sewage, which comprises the following steps:
(1) culture of anaerobic bacteria, aerobic bacteria: the preparation of culture medium: agar 1%, stalk powder 0.5%, peptone 1%, magnesium sulfate 0.3~0.8%, glucose 0.3~0.5%, potassium dihydrogen phosphate 0.3~0.8%, remaining be water, adjust pH value be 7.0~7.5, warp 110~130 DEG C of 20~25min of high-temperature sterilization are crossed, culture medium A, B, C are obtained;It is inoculated with anaerobic species by a certain percentage in desinfection chamber To culture medium A, it is inoculated in aerobic bacteria by a certain percentage in desinfection chamber to culture medium B, 24~32 DEG C of cultivation temperature, incubation time 24 ~50 hours;
(2) it strain domestication, hot housing: is put into respectively in aerobic strain pond and anaerobic species pond from secondary sedimentation tank of sewage treatment work Sludge 20~30%, magnesium sulfate 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.5%, the multi-vitamins 0.5~1%, Portugal of acquirement Grape sodium saccharate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2%, waste water, then add cultivate respectively Aerobic strain and anaerobic species;The waste water of pond body product 40~45%, stirring are first put into strain pond;Pond body product 6 is added daily ~8% waste water, until reaching ordinary water level, aerobic and anaerobic sludge after being tamed, being strengthened, and launched together into anaerobism In reaction tank;
(3) it the culture of flocculated bacteria: is inoculated with the strain of flocculation ability: rose pink mold cyst bacterium, Fei Lv by a certain percentage in desinfection chamber Guest's actinoplanes, inoculative proportion are 1:1 to culture medium C, 24~32 DEG C of cultivation temperature, incubation time 24~50 hours;Expand again Big hot housing expands hot housing based component are as follows: sucrose 1%, agar 1%, potassium nitrate 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.5%, sodium chloride 0.5%, sodium gluconate 1~2%, glycerin monostearate 1~2%, n,N-dimethylacetamide 1~2%, Adjusting pH value is 7.0~7.5;Shaking table is opened, cultivation temperature is 30 DEG C, incubation time 48h;Bacterium after will be enlarged by hot housing Strain culture medium elder generation high speed centrifugation obtains supernatant as flocculation microbial inoculum;
(4) sewage is again thick to 8 layers of thin grid by grid gap, removes bulky grain solid impurity;It enters back into and overregulates pond adjusting pH It is 6~7.5;
(5) sewage goes successively to flocculation basin, and the flocculation microbial inoculum of sewage volume 2~5%, stirring is added, and 7~9h of hydraulic retention removal is hanged Floating object and some of the contaminants;Flocculation basin side wall installs aerator, and middle part is equipped with blender, and bottom is and water (flow) direction phase The anti-gradient, to collect and be discharged precipitating;
(6) flocculation basin supernatant enters anaerobic reation pool, and 8~10h of hydraulic retention is stirred, and good bacteria can consume extra oxygen point Pollutant is solved, while providing anaerobic environment to anaerobic bacteria to make anaerobic bacteria decomposing pollutant, the methane recovery generated during this is extremely Anaerobic reation pool connects methane recovery pond;
(7) sewage continues to flow into aerobic aeration biofilter from bottom, is aerated in supporting layer, and supporting layer filtrate is 3~8cm of diameter Cobblestone;It next is the cross nylon filtering cloth of first layer, placing filtrate is the long 3~5cm of special-shaped ceramics, and mix bacterium agent is being filtered Biomembrane is formed on cloth and continues decomposing pollutant, while the structure of filter cloth plays a role in filtering;The second layer is trilobal weave Buddhist nun Imperial filter cloth filtrate is the long 1~3cm of special-shaped ceramics, and third layer is pentagon weave nylon filtering cloth, active carbon, 5~100 mesh, until under Up from thick to thin;Sewage is discharged into filtering ponds by gravity secondary filter from top, by 60~200 mesh of active carbon, by It is arrived carefully up to lower to be thick, bottom is the double-deck five-pointed star weave nylon cloth, and in top and bottom, installation ultraviolet lamp carries out disinfection, finally It is discharged after detection, then returns to anaerobic reation pool if it does not meet the standards;
Sewage disposal device includes: aerobic seeding tank, anaerobic species tank, grid, conditioning tank, flocculation basin, anaerobic reation pool, aerobic Biological aerated filter, methane recovery device.
2. a kind of microbial inoculum treatment process of town sewage according to claim 1, it is characterised in that: anaerobic species are to produce first The combination of alkane bacillus, methane phase coccus, Bacillus acidi lactici.
3. a kind of microbial inoculum treatment process of town sewage according to claim 2, it is characterised in that: methane phase bacillus produces Methanosarcina, Bacillus acidi lactici inoculative proportion are 2:2:3.
4. a kind of microbial inoculum treatment process of town sewage according to claim 1, it is characterised in that: aerobic strain is poly- phosphorus The combination of bacterium, hydrolysis acidification bacterium, saccharomycete.
5. a kind of microbial inoculum treatment process of town sewage according to claim 4, it is characterised in that: polyP bacteria, hydrolysis acid Change bacterium, the inoculative proportion of saccharomycete is 3:2:5.
6. a kind of microbial inoculum treatment process of town sewage according to claim 4, it is characterised in that: polyP bacteria calcium acetate is not One or both of lever bacterium, Acinetobacter junii, Denitrifying Phosphate Accumulating Organisms, deep red red ring bacterium.
7. a kind of microbial inoculum treatment process of town sewage according to claim 4, it is characterised in that: hydrolysis acidification bacterium is wax One or both of shape bacillus, starch Clostridium, succinic acid bacteroid.
8. a kind of microbial inoculum treatment process of town sewage according to claim 1, it is characterised in that: stirring in step (2) Mixing rate is 400rpm~600rpm;Shaking table rate is 100~150r/min in step (3);Stirring rate is in step (5) 200rpm~400rpm;Stirring rate is 300rpm~500rpm in step (6).
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109735482A (en) * 2019-03-08 2019-05-10 刘耀红 A kind of aerobic strain activating agent and its preparation method and application method
CN113136351A (en) * 2021-04-25 2021-07-20 重庆融极浩瀚生物技术有限公司 Composite microbial flocculant and preparation method and application thereof
CN113292156A (en) * 2021-02-09 2021-08-24 深圳前海百润环保科技有限公司 Mixed growth type sewage treatment method and system
CN113307442A (en) * 2021-02-08 2021-08-27 广东创实环境科技有限公司 Fluidized bed biological membrane sewage treatment method and system
CN114031208A (en) * 2021-10-18 2022-02-11 广东众城生态环境发展有限公司 Industrial wastewater treatment process

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109735482A (en) * 2019-03-08 2019-05-10 刘耀红 A kind of aerobic strain activating agent and its preparation method and application method
CN113307442A (en) * 2021-02-08 2021-08-27 广东创实环境科技有限公司 Fluidized bed biological membrane sewage treatment method and system
CN113292156A (en) * 2021-02-09 2021-08-24 深圳前海百润环保科技有限公司 Mixed growth type sewage treatment method and system
CN113136351A (en) * 2021-04-25 2021-07-20 重庆融极浩瀚生物技术有限公司 Composite microbial flocculant and preparation method and application thereof
CN114031208A (en) * 2021-10-18 2022-02-11 广东众城生态环境发展有限公司 Industrial wastewater treatment process

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