CN108623008A - The method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light - Google Patents
The method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light Download PDFInfo
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- CN108623008A CN108623008A CN201810373486.1A CN201810373486A CN108623008A CN 108623008 A CN108623008 A CN 108623008A CN 201810373486 A CN201810373486 A CN 201810373486A CN 108623008 A CN108623008 A CN 108623008A
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- microalgae
- syntaxial system
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/301—Aerobic and anaerobic treatment in the same reactor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
The present invention provides the method that microalgae aerobic sludge granular syntaxial system is quickly formed under natural light, which is characterized in that including：It is inoculated with ripe aerobic particle mud in the reactor；Reactor is placed at southern exposure, receives natural lightings in 10 hours or more daily, using aerobic/anaerobic/anoxic mode operation, aerobic/anaerobic/anoxic pattern is：Water inlet 2~10min, anaerobism 100~150min, aerobic 90~120min, 60~164min of anoxic, settle 2~10min, drain 2~10min；The aeration quantity of aerobic section is 270~330mL/min；Mixing speed is 200~300r/min；Ripe microalgae aerobic sludge granular syntaxial system is obtained to 7d.This method substantially reduces the incubation time of microalgae aerobic sludge granular in the prior art；Promote wastewater purifying efficiency and impact resistance；Realize the self-produced oxygen operation of syntaxial system.
Description
Technical field
The invention belongs to bioengineering, field of environment engineering technology, and in particular to quickly form microalgae-under a kind of natural light
The method of aerobic sludge granular syntaxial system.
Background technology
The two large problems urgently solved needed for 21 century human social：Environmental pollution and energy crisis.It is dirty
The features such as aquatic organism treatment technology is of low cost, secondary pollution is small with its has as the important component of water treatment field
Important engineering application value.Wherein aerobic particle mud is due to biomass is big, stable structure, settling property are good, resistance to punching
It hits load, the advantages that sludge activity is high, is used widely in the biological treatment of sanitary sewage.Microalgae has growth cycle
Advantage short, biological yield is high, the triglyceride (Triacylglycerols, TAG) in cell grease is to prepare biology
Therefore the primary raw material of diesel oil, microalgae are considered as preparing biodiesel most to have the nontoxic regenerative resource of development potentiality.Microalgae-
Aerobic sludge granular can realize that energy recovery utilizes for handling sanitary sewage on the basis of reaching efficient stable decontamination,
It solves the problems, such as that the high energy consumption of generally existing, efficiency are low in existing desmear process and carbon emission amount is big, and obtains abundant biology
Mass-energy source.
Microalgae-aerobic sludge granular syntaxial system has been shown to improve denitrogenation dephosphorizing efficiency, but existing structure is micro-
The startup week of technology generally existing microalgae-aerobic sludge granular syntaxial system reactor of algae-aerobic sludge granular syntaxial system
Phase generally grows the problem of (generally 35-45d) partially, and algae-bacterium pelletising process generally need to such as be borrowed by the effect of immobilization technology
Help the suction-operated of adsorbent or the embedding effect by means of embedding medium, and needs to give artificial light source mostly, therefore how
Quickly, low consumption obtains microalgae-aerobic sludge granular syntaxial system as a big key of Technique Popularizing application.
Invention content
The present invention is to carry out to solve the above-mentioned problems, and it is an object of the present invention to provide quickly being formed under a kind of natural light micro-
The method of algae-aerobic sludge granular syntaxial system.
The present invention to achieve the goals above, uses following scheme.
The present invention provides a kind of method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light, feature
It is, including：It is inoculated with process：The ripe aerobic particle mud of inoculation in sequencing batch reactor (SBR)；Syntaxial system forms work
Sequence：Sequencing batch reactor is not added with concealed ground to be placed at southern exposure, receives natural lightings in 10 hours or more daily, using detesting
Oxygen/aerobic/anoxic mode operation, aerobic/anaerobic/anoxic pattern are：It intakes 2~10min, anaerobism 100~150min, aerobic 90
~120min, 60~164min of anoxic settle 2~10min, drain 2~10min；The aeration quantity of aerobic section is 270~330mL/
min；Anaerobism section, aerobic section and anoxic section are stirred, and rotating speed is 200~300r/min；It is good that ripe microalgae-is obtained to 7d
Oxygen mud granule syntaxial system, later system running stablize, parameters all reach stable, and to the treatment effect of sewage
Reach stable.
Preferably, the side of microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light according to the present invention
In method, it can also have the feature that：In syntaxial system formation process, light application time is 10.33h~14.13h.
Preferably, the side of microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light according to the present invention
In method, it can also have the feature that：In syntaxial system formation process, light application time 14h, the intensity model of natural lighting
It encloses for 2000~90000lux.
It is quickly formed under natural light according to the present invention in the method for microalgae-aerobic sludge granular syntaxial system, may be used also
To have the feature that：In syntaxial system formation process, sequencing batch reactor runs 4 periods, each cycle operation daily
6h。
It is quickly formed under natural light according to the present invention in the method for microalgae-aerobic sludge granular syntaxial system, also
It can have the feature that：In syntaxial system formation process, daytime, highest water temperature was 38~42 DEG C in sequencing batch reactor,
Night water temperature is 23~25 DEG C.
It is quickly formed under natural light according to the present invention in the method for microalgae-aerobic sludge granular syntaxial system, also
It can have the feature that：In syntaxial system formation process, aerobic/anaerobic/anoxic pattern is：Water inlet 2min, anaerobism
120min, aerobic 90min, anoxic 144min settle 2min, drain 2min.
Preferably, the side of microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light according to the present invention
In method, it can also have the feature that：In sequencing batch reactor, water inlet is arranged in sequencing batch reactor height 50%
Place, mud discharging mouth setting are aerated by air pump from bottom in bottom, draining than being 50%.
Preferably, the side of microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light according to the present invention
In method, it can also have the feature that：Sequencing batch reactor is cylinder, ratio of height to diameter 5, dischargeable capacity 3.6L.
Preferably, the side of microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light according to the present invention
In method, further include：Water source preparation process：Water inlet water source simulated domestic wastewater is configured, COD (COD) is in the water source that intakes
200mg/L、NH4 +- N is 20mg/L, TP 5mg/L.
Preferably, the side of microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light according to the present invention
In method, it can also have the feature that：In being inoculated with process, the calcium ions and magnesium ions and 1mL/L of 10mg/L are added into water inlet
Micro solution, micro solution includes：The FeCl of 0.9mg/L3, the H of 0.15mg/L3BO3, the CuSO of 0.03mg/L4·5H2O,
The MnCl of the KI of 0.18mg/L, 0.06mg/L2·4H2The Na of O, 0.06mg/L2Mo7O24·4H2The ZnSO of O, 0.12mg/L4·
7H2The CoCl of O, 0.15mg/L2·6H2The EDTA of O and 10mg/L.
The effect of invention
The method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light provided by the invention, in natural light
Under conditions of, weak aeration condition and settling time, the sbr reactor device that is run in a manner of aerobic/anaerobic/anoxic (A/O/A)
Mature and stable microalgae-aerobic sludge granular syntaxial system is can get in 7d.The present invention substantially reduces previously reported micro-
Algae-aerobic sludge granular incubation time；Promote wastewater purifying efficiency and impact resistance；Realize that microalgae-aerobic sludge granular is total
Raw body is self-produced oxygen operation, reduces the energy consumption that additional light source is generated with aeration；Improve the heat of excess sludge in sewage disposal system
Value and fat content, promote its recycling, are quick startup, the dirt of microalgae-aerobic sludge granular syntaxial system reactor
Water warfare and resource reclaim provide new thinking.
This method, which is formed by microalgae-aerobic sludge granular syntaxial system, can be effectively treated sanitary sewage, effluent quality
Reach level-one A marks, meets sanitary sewage discharge standard, and syntaxial system excess sludge be discharged can also be used as biological bavin
Raw material prepared by oil, biogasoline, fermentation and hydrogen production methane phase, biological organic fertilizer and biological carbon.
Description of the drawings
Fig. 1 be the aerobic particle mud of 1d, 3d, 7d in embodiment aspect graph (A, a and B, b) and microalgae-it is good
The aspect graph (C, c and D, d) of oxygen mud granule；
Fig. 2 is microalgae in embodiment-aerobic sludge granular forming process (3d) and ripe microalgae-aerobic sludge granular
Microbial population in (7d)：A is the other bacterial community of gate leve；B is the bacterial community of guiding principle rank；C is the eukaryon of guiding principle rank
Algae.
Fig. 3 is NH of microalgae in the embodiment-aerobic sludge granular syntaxial system to sanitary sewage4 +-N(a)、TP(b)、COD
(c) removal effect figure.
Specific implementation mode
Referring to the drawings to quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to the present invention
Method be elaborated.
<Embodiment>
(1) water inlet water source is prepared
Water inlet water source is the simulated domestic wastewater of laboratory configuration, and human configuration is into chemistry of aqueous solution oxygen demand (COD)
200mg/L、NH4 +- N is 20mg/L, TP 5mg/L, pH value 7.5.
(2) it is inoculated with aerobic particle mud
The ripe aerobic particle mud that inoculated and cultured obtains in sequencing batch reactor (SBR) should as shown in Figure 1A and a
Aerobic particle mud is in orange-yellow, and surface is smooth, and appearance is spherical or elliposoidal, and average diameter is 1.77 ± 0.47mm, aerobic
Granule sludge includes mainly C, H, O, N, S, P totally 6 kinds of elements, and MLSS is 7800 ± 800mg/L, and MLVSS is 6600 ± 200mg/
LSVI5 is 33.17 ± 3.81mL/g, and specific oxygen utilization rate is 18.59 ± 0.8mgO2/(gMLVSS·h)；It can from Figure 1B and b
Go out, ripe aerobic particle mud surface is controlled by bacillus.In sequencing batch reactor, ripe aerobic particle mud is to water inlet
COD、NH4 +The removal rate of-N and TP is respectively 93%, 91.2% and 95.8%, and dominant bacteria is deformation in ripe aerobic particle mud
Bacterium door (Alpha-, Beta-, Gamma- and Deltaproteobacteria), green curved bacterium door (Anaerolineae
Chloroflexia and Ardenticatenia) and Bacteroidetes (Sphingobacteriia, Flavobacteriia and
Bacterioidetes)；Calcium ions and magnesium ions and 1mL/L that 10mg/L is added also are needed to maintain the shape of aerobic grain, in water inlet
Micro solution.Include specifically in micro solution：The FeCl of 0.9mg/L3, the H of 0.15mg/L3BO3, the CuSO of 0.03mg/L4·
5H2The MnCl of the KI of O, 0.18mg/L, 0.06mg/L2·4H2The Na of O, 0.06mg/L2Mo7O24·4H2O, 0.12mg/L's
ZnSO4·7H2The CoCl of O, 0.15mg/L2·6H2The EDTA of O and 10mg/L.
Specific incubation is in sequencing batch reactor：By the mud cake for being derived from sewage treatment plant's evaporation plant by sieving and washing
Except the bored exposure of simulated domestic wastewater 1~2 day is added after impurity, the sludge after a certain amount of bored exposure is taken to be added in sequencing batch reactor, then
Water inlet water source to reactor dischargeable capacity, which is added, makes sludge concentration be 5000mg/L, using aerobic/anaerobic/anoxic (A/O/A) mould
Formula is run, and runs 4 periods, each cycle 6h daily, and aerobic/anaerobic/anoxic pattern is：Water inlet 1min, anaerobism 20min, aeration
100min, anoxic 20min settle 2min, drain 1min；Dissolved oxygen in reactor is：Anaerobism 0.10 ± 0.05mg/L of section exposes
Gas 5.0 ± 0.05mg/L of section, anoxic 0.30 ± 0.05mg/L of section, aeration section flow are 2.4L/min.
Here the aerobic particle mud used mainly contains microorganism and Extracellular polymers, main group of aerobic particle mud
It is the metallic elements such as C, H, O, N, S, P and a small amount of Ca, Mg, Fe at element.
(3) microalgae-aerobic sludge granular fast culture
Reactor is not added with concealed ground in summer to be placed on towards by the window in south, about receives 12 hours natural lights daily
According to (6:30-18:30), intensity of illumination range is in 2000~90000lux.It is transported using aerobic/anaerobic/anoxic (A/O/A) pattern
Row, runs 4 periods daily, and each cycle runs 6h；Aerobic/anaerobic/anoxic pattern is：It intakes 2min, anaerobism 120min, it is aerobic
90min, anoxic 144min settle 2min, drain 2min；The aeration quantity of aerobic section is 300mL/min；Anaerobism section, aerobic section and
Anoxic section is stirred, rotating speed 250r/min；Continue to monitor water temperature, daytime about 38~42 DEG C of highest water temperature, night water temperature is about
23~25 DEG C；Stable microalgae-aerobic sludge granular syntaxial system is formed to 7d.7d is can be seen that from Fig. 1 D and d to obtain
Ripe microalgae-aerobic sludge granular present bottle green graininess, observed under scanning electron microscope and be characterized as surface because simultaneously
There are trichobacteria and algae and becomes more coarse.As can be seen that the aerobic dirt of ripe microalgae-that 7d is obtained from Fig. 2A and B
The other dominant bacteria of gate leve in mud particle is：Proteobacteria (Alpha-, Beta-, Gamma- and
Deltaproteobacteria), green curved bacterium door (Anaerolineae Chloroflexia and Ardenticatenia) and intend
Bacillus door (Sphingobacteriia, Flavobacteriia and Bacterioidetes).It can be seen that maturation from Fig. 2 C
The dominant bacteria of guiding principle rank in microalgae-aerobic sludge granular.In addition, the advantage algae of microalgae is green alga.
Sanitary sewage can be effectively treated in the microalgae of formation-aerobic sludge granular syntaxial system, the removal effect reflected from Fig. 3
Fruit figure can be seen that NH of the microalgae-aerobic particle mud syntaxial system to sanitary sewage4 +- N, TP, COD removal rate are high, and go out
Water water quality reaches level-one A marks, meets sanitary sewage emission request.Further, the excess sludge of discharge can be used as biodiesel, life
Raw material prepared by object gasoline, fermentation and hydrogen production methane phase, biological organic fertilizer and biological carbon.Take microalgae-aerobic particle mud symbiosis
System MLSS is 4.0g/L, and grease MLSS is 2.0g/L, and microalgae grease yield takes 40%, you can obtains 20% lipid-producing.
Above example is only the illustration done to technical solution of the present invention.Under natural light according to the present invention
The method for quickly forming microalgae-aerobic sludge granular syntaxial system is not merely defined in described knot in the embodiment above
Structure, but it is subject to claim limited range.Those skilled in the art of the invention are in the embodiment on the basis of institute
Any modify or supplement or equivalence replacement done, all in the claim range claimed of the present invention.
Claims (10)
1. a kind of method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light, which is characterized in that including：
It is inoculated with process：Aerobic particle mud is inoculated in sequencing batch reactor；
Syntaxial system formation process：Sequencing batch reactor is not added with concealed ground to be placed at southern exposure, receive 10 hours daily or more
Natural lighting, using aerobic/anaerobic/anoxic mode operation, aerobic/anaerobic/anoxic pattern is：2~10min of water inlet, anaerobism
100~150min, aerobic 90~120min, 60~164min of anoxic settle 2~10min, drain 2~10min；Aerobic section
Aeration quantity is 270~330mL/min；Anaerobism section, aerobic section and anoxic section are stirred, and rotating speed is 200~300r/min；To
7d obtains ripe microalgae-aerobic sludge granular syntaxial system.
2. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 1, special
Sign is：
Wherein, in syntaxial system formation process, light application time is 10.33h~14.13h.
3. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 1, special
Sign is：
Wherein, in syntaxial system formation process, light application time 14h, the strength range of natural lighting is 2000~
90000lux。
4. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 1, special
Sign is：
Wherein, in syntaxial system formation process, sequencing batch reactor runs 4 periods daily, and each cycle runs 6h.
5. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 1, special
Sign is：
Wherein, in syntaxial system formation process, daytime, highest water temperature was 38~42 DEG C in sequencing batch reactor, and night water temperature is
23~25 DEG C.
6. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 1, special
Sign is：
Wherein, in syntaxial system formation process, aerobic/anaerobic/anoxic pattern is：It intakes 2min, anaerobism 120min, it is aerobic
90min, anoxic 144min settle 2min, drain 2min.
7. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 1, special
Sign is：
Wherein, in sequencing batch reactor, water inlet is arranged at sequencing batch reactor height 50%, and mud discharging mouth is arranged the bottom of at
Portion, draining are aerated by air pump from bottom than being 50%.
8. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 7, special
Sign is：
Wherein, sequencing batch reactor is cylinder, ratio of height to diameter 5, dischargeable capacity 3.6L.
9. the method for quickly forming microalgae-aerobic sludge granular syntaxial system under natural light according to claim 1, special
Sign is, further includes：
Water source preparation process：Water inlet water source simulated domestic wastewater is configured, COD is 200mg/L, NH in the water source that intakes4 +-N
For 20mg/L, TP 5mg/L.
10. the method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light according to claim 1,
It is characterized in that：
Wherein, in being inoculated with process, the calcium ions and magnesium ions of 10mg/L and the micro solution of 1mL/L are added into water inlet,
The micro solution includes：The FeCl of 0.9mg/L3, the H of 0.15mg/L3BO3, the CuSO of 0.03mg/L4·5H2O, 0.18mg/
The MnCl of the KI of L, 0.06mg/L2·4H2The Na of O, 0.06mg/L2Mo7O24·4H2The ZnSO of O, 0.12mg/L4·7H2O,
The CoCl of 0.15mg/L2·6H2The EDTA of O and 10mg/L.
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| CN111689576A (en) * | 2020-05-29 | 2020-09-22 | 深圳市万创青绿环境工程有限公司 | Reactor for preparing algae granular sludge and method for culturing algae granular sludge |
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| CN110655183A (en) * | 2019-07-31 | 2020-01-07 | 武汉大学 | Method for strengthening aerobic sludge granulation and decontamination performance by weak magnetic field |
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| CN111533268B (en) * | 2020-04-16 | 2023-02-17 | 武汉科技大学 | Algae-bacterium particle, preparation method thereof and method for treating domestic sewage by using same |
| CN111689576A (en) * | 2020-05-29 | 2020-09-22 | 深圳市万创青绿环境工程有限公司 | Reactor for preparing algae granular sludge and method for culturing algae granular sludge |
| CN111689576B (en) * | 2020-05-29 | 2022-07-05 | 深圳市万创青绿环境工程有限公司 | Reactor for preparing algae granular sludge and method for culturing algae granular sludge |
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Application publication date: 20181009 |