CN104370374A - Method for denitrogenation and phosphorus-removal of town sewage through efficient immobilized algae bacterial symbiosis system - Google Patents
Method for denitrogenation and phosphorus-removal of town sewage through efficient immobilized algae bacterial symbiosis system Download PDFInfo
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- CN104370374A CN104370374A CN201410469741.4A CN201410469741A CN104370374A CN 104370374 A CN104370374 A CN 104370374A CN 201410469741 A CN201410469741 A CN 201410469741A CN 104370374 A CN104370374 A CN 104370374A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
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Abstract
The present invention discloses an efficient town sewage denitrogenation and phosphorus-removal biological treatment method, and belongs to the technical field of sewage treatment. According to the method, the dominant algae variety identified from the municipal wastewater treatment plant is selected as the algae source, active sludge is adopted as the bacterial source, an elastic three-dimensional filler is adopted as the immobilized filler, and the sewage is treated according to an algae bacterial dry weight mass ratio of 1:5-5:1, an illumination intensity of 4000-6000 lx, and a hydraulic detention time of 2-3 d. According to the present invention, the algae and bacteria immobilization way is simple, the town sewage treatment effect is good, the indexes of ammonia nitrogen (NH4-N), total nitrogen (TN), total phosphorus (TP), chemical oxygen demand (CODCr) and suspended solids (SS) in the treated town sewage achieve the first grade A standard of the municipal wastewater treatment plant pollutant discharge standard (GB18918-2002). .
Description
Technical field
The invention belongs to technical field of sewage, relate to a kind of bioremediation of efficient town sewage denitrogenation dephosphorizing.
Background technology
Day by day serious along with problems such as body eutrophications, sewage discharge and improvement are subject to extensive concern.During 12, China improves town sewage water quality emission standard, and namely urban wastewater treatment firm pollutant emission standard (GB18918-2002) rises to one-level A by one-level B.Total nitrogen emission standard is reduced to 15mg/L by 20mg/L, and ammonia nitrogen emission standard is reduced to 5mg/L by 8mg/l in summer, and winter, 15mg/L was reduced to 8mg/L, and total phosphorus emission standard is reduced to 0.5mg/L by 1mg/L.
Biological Nitrogen Removal Processe owing to having the material such as carbon, nitrogen, phosphorus removed in sewage simultaneously, and can utilize biomass to be used widely as advantages such as the energy after disposing.Biological Nitrogen Removal Processe conventional is at present activated sludge process.
Although, there is following problem in traditional active sludge biological method technology maturation: (1) aeration tank volume is large, and floor space is many, and initial cost is many; (2) be difficult to synchronously realize high-efficient denitrification and dephosphorization efficiency; (3) be difficult under low dissolved axygen realize simultaneous nitrification and denitrification; (4) activated sludge bulking and settling property instability; (5) problem such as capacity of resisting impact load is poor.In order to promote the progress of process for town sewage treatment, and meeting more strict emission standard, being necessary to explore town sewage novel treatment technique.
Immobilized algal-bacteria syntaxial system is a kind of sewage treatment process of resource-conserving, synchronously can remove the material such as carbon, nitrogen, phosphorus in sewage, and denitrogenation dephosphorizing efficiency is high, immobilization algae, bacterium are easy to reclaim, for urban wastewater treatment provides a kind of new approaches.
Relate to more algae in current correlative study and invention, bacteria immobilization technology mainly adopts entrapping method to form the mode of immobilized spherule, for the preponderant algae that immobilized algae also not identifies in actual sewage." the utilizing immobilized algal-bacteria coupled system synchronously to remove COD in sewage and nitrogen phosphorus " that be published in " environmental science " the 32nd volume the 8th phase in 2011 as Deng Xu etc. adopts alginate calcium to be fixation support, and use algae is Chlamydomonas reinhardtii; Mao Shuduan etc. are published in " environmental engineering journal " the 6th volume the 5th phase in 2012 " waste water nutrition comparison immobilized algal-bacteria removes Pollutant effects and dynamics research " is fixation support with alginate calcium, uses algae to be micro-algae; Chinese Patent Application No. 200710168917.2, application publication date is on September 10th, 2008, invention and created name is denitrification and dephosphorization method and the device of the sepn process of immobilized algal-bacteria coupled film, described frustule immobilization material is one in carrageenin, alginate calcium, polyvinyl alcohol or polyacrylamide or arbitrary combination, and use algae is chlamydomonas, chlorella or its combination.Dispose of sewage using above-mentioned document or patent chemical substance used as fixation support, be unfavorable for the advantage giving full play to algae, bacterium mutualism, easily occur carrier solubilizes phenomenon, cause treatment effect not ideal enough, and cost is higher, there is some problems in practice.
Summary of the invention
The present invention aims to provide a kind of bio-denitrifying sewage phosphorus removing method being easy to through engineering approaches application, in immobilized algal-bacteria process town sewage process, there is phycomycete immobilization process complexity to solving, carrier easily dissolves, processing cost is higher, the shortcoming of through engineering approaches operational difficulties, for the energy-saving and emission-reduction of urban wastewater treatment provide technical support.
In order to realize this purpose, technical scheme of the present invention is:
Efficient immobilized algal-bacteria syntaxial system, to a method for town sewage denitrogenation dephosphorizing, the steps include:
(1) adopting polymerase chain reaction-denaturing gradient gel electrophoresis method to identify coenosis in urban wastewater treatment firm sewage, with the algae kind identified for algae source, take active sludge as bacterium source;
(2) control initial total biological dry weight concentration 0.2 ~ 0.4g/L, algae, bacterium to be added in reaction vessel by the dry weight ratio of wastewater treatment efficiency the best and algae, bacterium are fixed on elastic solid filler;
(3) add light source to reaction vessel, controlling the dark time ratio of light is 12h: 12h, intensity of illumination 4000 ~ 6000lx;
(4) simulated sewage or urban wastewater treatment firm sewage is added to reaction unit, sewage hydraulic detention time 2 ~ 3d.
Further, step (1) algae source is preferably easy to the Oedogonium filamentous algae of apposition growth;
Further, immobilization Oedogonium filamentous algae selects short strip sheath algae.
Further, the algae dry weight of step (2) wastewater treatment efficiency the best: bacterium dry weight scope is 1: 5 ~ 5: 1.
Further, step (2) phycomycete immobilization way by adopt to containing algae, bacterium water body stir or shake make water body flow reach algae, bacterium attachment fixed growth on elastic solid filler.
Further, the interpolation of step (3) light source is by arranging light source or being passed through in transparent hollow post insertion reaction container by light source at transparent reaction container surroundings.
The invention has the advantages that:
(1) this algaebacteria symbiotic system compares pure algae, pure bacterium has better removal effect to sewage.At suitable phycomycete dry weight than under condition, ammonia nitrogen (NH in town sewage after treatment
4-N), total nitrogen (TN), total phosphorus (TP), chemical oxygen demand (COD) (COD
cr), suspended solid (SS) index reaches one-level A standard in urban wastewater treatment firm pollutant emission standard (GB18918-2002).
(2) select qualification from the advantage algae kind short strip sheath algae of actual sewage treatment plant for immobilized algae kind, be conducive to improving nitrogen phosphorus ligands efficiency in sewage.This algae is filamentous algae, is very easily attached on filler, and for bacterium apposition growth provides carrier, thus process for fixation is with low cost, easy to implement.
(3) phycomycete immobilization is disposed of sewage, and phycomycete not easily runs off, and ensures process effluent quality, and is conducive to the recovery of algae, bacterium biomass, for the recycling processing artifact mass-energy source provides condition.
(4) in the method process waste water process, give full play to the advantage of phycomycete mutualism, realize synchronous carbon, nitrogen, the phosphorus removed in sewage, and be still suitable for when influent COD cr concentration is low, through engineering approaches uses prospect good.
Embodiment
Embodiment 1
From certain secondary sedimentation tank of sewage treatment work water sampling, according to polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) molecular biology method, the coenosis in sewage is analyzed, result shows, and there is preponderant algae: Oedogonium filamentous algae in sewage---short strip sheath algae (Oedogonium brevicingulatum) and asymmetric chlamydomonas (Chlamydomonas asymmetrica).
Two kinds of algae immobilization effects are evaluated: get 4,2L beaker, elastic solid filler is loaded in each beaker, packing volume filling ratio 60%, the short strip sheath algae of 0.5g dry weight is added respectively toward wherein two beakers, the asymmetric chlamydomonas of 0.5g dry weight is added respectively in another two beakers, each beaker adds 1.5L tap water, beaker is placed in shaking table, control water volume flow rate 30-60cm/s, intensity of illumination 4000lx, the light dark time takes out water sample 1000ml than for beaker each after 12h: 12h, 24 hours, measures wherein algae content.Known by measurement result: short strip sheath algae immobilization effect is obviously better than asymmetric chlamydomonas, short strip sheath algae fixed rate reaches 99% ~ 100%, and asymmetric chlamydomonas fixed rate is only 2% ~ 5%.Therefore preferably short strip sheath algae is the algae source of immobilized algal-bacteria system.
Short strip sheath algae dry weight defining method is: with the short strip sheath algae of quantitative paper sifting weighing one group of different volumes of drying in advance, first weigh and determine this group short strip sheath algae weight in wet base quality respectively, then this group algae is placed in 103 ± 1 DEG C of baking ovens to toast, repeatedly dry weighing, determine dry weight, with algae weight in wet base for X-coordinate, dry weight is that ordinate zou drafting short strip sheath algae does weight in wet base affinity criterions curve.According to typical curve, the short strip sheath algae of certain weight in wet base quality can be converted into dry weight.
Bacterium dry weight defining method: accurately measure 100mL active sludge, filter with drying weighed quantitative paper in advance, then the active sludge after filtering and filter paper are together placed in 103 ± 1 DEG C of baking ovens toast, repeatedly dry weighing, determine its dry weight, bacterium dry weight can be calculated thus.
Get 2L glass beaker 6, be placed on six magnetic stirring apparatuss, fill 15 ~ 20cm elastic solid filler, volumetric filling ratio 50% ~ 80%, common 25 watts of fluorescent tubes 2 ~ 3 are arranged around beaker, intensity of illumination 4000 ~ 6000lx, the light dark time is than 12h: 12h, initial total biological dry weight concentration 0.3g/L, according to pure bacterium, algae dry weight: bacterium dry weight be 1: 5,1: 1,5: 1,10: 1 and pure algae six experimental group add corresponding algae, bacterium, the simulated sewage of 2L is added, wherein containing NH in each beaker
4-N:20 ± 0.5mg/L, TP:4 ± 0.5mg/L, COD
cr: 250 ± 5mg/L, pH:7.58, water temperature: 25 ± 5 DEG C, whole process magnetic agitation, rotor speed control precipitates without algae and bacterium in beaker bottom.With NH
4-N, TN, TP, COD
cr, pH, SS be monitoring index, through 3d process, algae dry weight: bacterium dry weight scope is the experimental group of 1: 5 ~ 5: 1, NH in the simulated sewage after process
4-N, TN, TP, COD
cr, pH, SS all reach urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard.
Water quality in embodiment 1 after the initial content of each material, each experimental group process and the Data Comparison of urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard as follows:
Note: SS initial content is the phycomycete dry weight concentration initially added, and in simulated sewage, SS is 0.
Embodiment 2
Advantage algae qualification in sewage in the present embodiment, the screening of algae source, phycomycete dry weight determine and experimental group arrange all identical with embodiment 1, each beaker adds simulated sewage water quality NH
4-N:12 ± 0.5mg/L, TP:2 ± 0.2mg/L, COD
cr: 250 ± 5mg/L, pH:7.44, water temperature: 25 ± 5 DEG C.Through 3d process, algae dry weight: bacterium dry weight scope is the experimental group of 1: 5 ~ 5: 1, NH in the simulated sewage after process
4-N, TP, COD
cr, pH, SS all reach urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard.
The Data Comparison of the water quality after the initial content of each material in above-described embodiment, each experimental group process and urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard is as follows:
Note: SS initial content is the phycomycete dry weight concentration initially added, and in simulated sewage, SS is 0.
Can be drawn by embodiment 1 and the SS data in embodiment 2, the method that this immobilized algal-bacteria is disposed of sewage, phycomycete immobilization effect is good.From NH
4-N, TN, TP, COD
crdata can show that helotisn has better treatment effect than single pure algae, pure bacterium to sewage, and at algae dry weight: bacterium dry weight is in 1: 5 ~ 5: 1 scope, after this immobilized algal-bacteria system carries out 3d process to simulated sewage, and NH in sewage
4-N, TN, TP, COD
cr, pH, SS all reach urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard.
Embodiment 3
In the present embodiment, advantage algae qualification in sewage, the screening of algae source and phycomycete dry weight are determined identical with embodiment 1, control initial total biomass 0.3g/L, by algae dry weight: bacterium dry weight is 1: 5,1: 1,5: 1 arrange experimental group, elastic solid filler volumetric filling ratio 50% ~ 80%, controlled light intensity 4000-6000lx, the light dark time is than 12h: 12h, using certain a sewage treatment plant inflow as experimental subjects, whole process magnetic agitation, rotor speed control precipitates without algae and bacterium in beaker bottom.Through 2d process, NH in experiment water
4-N, TN, TP, COD
cr, pH, SS all reach urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard.
The Data Comparison of water quality, corresponding clearance and urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard after the waste water quality adopted in above-described embodiment, process is as follows:
Note: SS is content in urban wastewater treatment firm water inlet.
Embodiment 4
In the present embodiment, advantage algae qualification in sewage, the screening of algae source and phycomycete dry weight are determined identical with embodiment 1, and using certain b sewage treatment plant inflow as experimental subjects, all the other embodiments are identical with embodiment 3, through 3d process, and NH in experiment water
4-N, TN, TP, COD
cr, pH, SS value all reaches urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard.
The Data Comparison of water quality, corresponding clearance and urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standard after the waste water quality adopted in above-described embodiment, process is as follows:
Note: SS is content in urban wastewater treatment firm water inlet.
Can be found out, at low concentration COD by embodiment 3, embodiment 4
crunder condition, the method is to town sewage good denitrogenation and dephosphorization effect, after treatment NH in sewage
4-N, TN, TP, COD
cr, pH, SS index all reaches one-level A standard, sewage treatment process and required condition simple, engineer applied exploitativeness is strong.
Claims (6)
1. efficient immobilized algal-bacteria syntaxial system is to a method for town sewage denitrogenation dephosphorizing, the steps include:
(1) adopting polymerase chain reaction-denaturing gradient gel electrophoresis method to identify coenosis in urban wastewater treatment firm sewage, with the algae kind identified for algae source, take active sludge as bacterium source;
(2) control initial total biological dry weight concentration 0.2 ~ 0.4g/L, algae, bacterium to be added in reaction vessel by the dry weight ratio of wastewater treatment efficiency the best and algae, bacterium are fixed on elastic solid filler;
(3) add light source to reaction vessel, controlling the dark time ratio of light is 12h: 12h, intensity of illumination 4000 ~ 6000lx;
(4) simulated sewage or urban wastewater treatment firm sewage is added to reaction unit, sewage hydraulic detention time 2 ~ 3d.
2. a kind of efficient immobilized algal-bacteria syntaxial system according to claim 1 is to the method for town sewage denitrogenation dephosphorizing, and it is characterized in that, step (1) algae source is preferably easy to the Oedogonium filamentous algae of apposition growth.
3. according to a kind of efficient immobilized algal-bacteria syntaxial system according to claim 1 or claim 2 to the method for town sewage denitrogenation dephosphorizing, it is characterized in that, immobilization Oedogonium filamentous algae selects short strip sheath algae.
4. a kind of efficient immobilized algal-bacteria syntaxial system according to claim 1 is to the method for town sewage denitrogenation dephosphorizing, it is characterized in that, the algae dry weight of step (2) wastewater treatment efficiency the best: bacterium dry weight scope is 1: 5 ~ 5: 1.
5. a kind of efficient immobilized algal-bacteria syntaxial system according to claim 1 is to the method for town sewage denitrogenation dephosphorizing, it is characterized in that, step (2) phycomycete immobilization way by adopt to containing algae, bacterium water body stir or shake make water body flow reach algae, bacterium attachment fixed growth on elastic solid filler.
6. a kind of efficient immobilized algal-bacteria syntaxial system according to claim 1 is to the method for town sewage denitrogenation dephosphorizing, it is characterized in that, the interpolation of step (3) light source by arrange common fluorescent tube at transparent reaction container surroundings or by common fluorescent tube by transparent hollow post insertion reaction container.
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| CN105174476A (en) * | 2015-09-28 | 2015-12-23 | 中国科学院城市环境研究所 | Activated sludge and microalgae coupled granular system for waste water treatment and establishment and operation method thereof |
| CN105776732A (en) * | 2016-02-19 | 2016-07-20 | 浙江清环保工程有限公司 | Enhanced nitrogen and phosphorus removal device and technology for sewage |
| CN104891678B (en) * | 2015-06-26 | 2016-09-07 | 内蒙古阜丰生物科技有限公司 | For administering the biologic product of the high-concentration waste water that amino acid fermentation produces |
| CN106277582A (en) * | 2016-08-19 | 2017-01-04 | 福建禹环境科技有限公司 | A kind of utilize microalgae method of denitrogenation dephosphorizing in aquaculture wastewater |
| CN108059247A (en) * | 2017-12-31 | 2018-05-22 | 温州大学 | A kind of sewage treatment unit |
| CN108163983A (en) * | 2018-01-17 | 2018-06-15 | 陈英 | A kind of method using compound algae preparation removal nitrogen and phosphorus pollutants |
| CN108623008A (en) * | 2018-04-24 | 2018-10-09 | 武汉大学 | The method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light |
| CN109234167A (en) * | 2018-10-10 | 2019-01-18 | 中国农业大学 | A kind of chlorella and its application in biogas slurry purification |
| CN109942091A (en) * | 2019-04-09 | 2019-06-28 | 哈尔滨学院 | Bacterium algae attaching biological fibre bed and preparation method thereof and its method of intensive treatment pig denitrogenation of waste water dephosphorization |
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| CN113979509A (en) * | 2021-11-05 | 2022-01-28 | 北京农学院 | Application of ultrathin sheet metal hydroxide in antibiotic degradation |
| CN117965311A (en) * | 2024-04-01 | 2024-05-03 | 山东悦翔生物有限公司 | Immobilization method of microalgae |
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| CN105174476B (en) * | 2015-09-28 | 2017-08-01 | 中国科学院城市环境研究所 | Activated sludge and microalgae coupling particle system and its structure and operation method for wastewater treatment |
| CN105174476A (en) * | 2015-09-28 | 2015-12-23 | 中国科学院城市环境研究所 | Activated sludge and microalgae coupled granular system for waste water treatment and establishment and operation method thereof |
| CN105776732A (en) * | 2016-02-19 | 2016-07-20 | 浙江清环保工程有限公司 | Enhanced nitrogen and phosphorus removal device and technology for sewage |
| CN106277582A (en) * | 2016-08-19 | 2017-01-04 | 福建禹环境科技有限公司 | A kind of utilize microalgae method of denitrogenation dephosphorizing in aquaculture wastewater |
| CN108059247A (en) * | 2017-12-31 | 2018-05-22 | 温州大学 | A kind of sewage treatment unit |
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| CN108163983A (en) * | 2018-01-17 | 2018-06-15 | 陈英 | A kind of method using compound algae preparation removal nitrogen and phosphorus pollutants |
| CN108623008A (en) * | 2018-04-24 | 2018-10-09 | 武汉大学 | The method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light |
| CN109234167A (en) * | 2018-10-10 | 2019-01-18 | 中国农业大学 | A kind of chlorella and its application in biogas slurry purification |
| CN109234167B (en) * | 2018-10-10 | 2021-01-15 | 中国农业大学 | Chlorella and application thereof in biogas slurry purification |
| CN109942091A (en) * | 2019-04-09 | 2019-06-28 | 哈尔滨学院 | Bacterium algae attaching biological fibre bed and preparation method thereof and its method of intensive treatment pig denitrogenation of waste water dephosphorization |
| CN109942091B (en) * | 2019-04-09 | 2022-04-29 | 哈尔滨学院 | Bacterial-algae attached biological fiber bed, preparation method thereof and method for strengthening treatment of pig wastewater for nitrogen and phosphorus removal |
| CN110272123A (en) * | 2019-07-03 | 2019-09-24 | 湘潭大学 | A method of immobilized algal-bacteria syntaxial system denitrogenation dephosphorizing efficiency is improved with inorganic carbon source |
| CN113979509A (en) * | 2021-11-05 | 2022-01-28 | 北京农学院 | Application of ultrathin sheet metal hydroxide in antibiotic degradation |
| CN113979509B (en) * | 2021-11-05 | 2023-11-10 | 北京农学院 | Application of ultrathin sheet metal hydroxide in antibiotic degradation |
| CN117965311A (en) * | 2024-04-01 | 2024-05-03 | 山东悦翔生物有限公司 | Immobilization method of microalgae |
| CN117965311B (en) * | 2024-04-01 | 2024-06-25 | 山东悦翔生物有限公司 | Immobilization method of microalgae |
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