CN104370374B - A kind of efficiently method of the immobilized algal-bacteria syntaxial system to town sewage denitrogenation dephosphorizing - Google Patents
A kind of efficiently method of the immobilized algal-bacteria syntaxial system to town sewage denitrogenation dephosphorizing Download PDFInfo
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- CN104370374B CN104370374B CN201410469741.4A CN201410469741A CN104370374B CN 104370374 B CN104370374 B CN 104370374B CN 201410469741 A CN201410469741 A CN 201410469741A CN 104370374 B CN104370374 B CN 104370374B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
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Abstract
The invention discloses a kind of bioremediation of efficient town sewage denitrogenation dephosphorizing, belong to technical field of sewage.This method is chosen and identifies that from the advantage algae kind of urban wastewater treatment firm be algae source, using activated sludge as bacterium source, elastic solid filler is used as immobilization filler, according to phycomycete dry weight than 1: 5~5: 1,4000~6000lx of intensity of illumination, 2~3d of hydraulic detention time condition processing sewage.Algae of the present invention, bacteria immobilization mode are simple, through processing after in town sewage ammonia nitrogen (NH good to urban wastewater treatment effect4N), total nitrogen (TN), total phosphorus (TP), COD (CODCr), solid suspension (SS) index reach one-level A standards in urban wastewater treatment firm pollutant emission standard (GB18918 2002).
Description
Technical field
The invention belongs to technical field of sewage, is related to a kind of biological treatment side of efficient town sewage denitrogenation dephosphorizing
Method.
Background technology
With getting worse the problems such as body eutrophication, sewage discharge receives significant attention with improvement.12 phases
Between, China improves town sewage water quality discharge standard, i.e. urban wastewater treatment firm pollutant emission standard (GB18918-
2002) lifted by one-level B to one-level A.Total nitrogen discharge standard is reduced to 15mg/L by 20mg/L, and ammonia nitrogen discharge standard is by summer
8mg/l is reduced to 5mg/L, and winter 15mg/L is reduced to 8mg/L, and total phosphorus discharge standard is reduced to 0.5mg/L by 1mg/L.
Biological Nitrogen Removal Processe can profit due to removing the materials such as carbon in sewage, nitrogen, phosphorus simultaneously, and after disposing
It is used widely by the use of biomass as the advantages that the energy.Currently used Biological Nitrogen Removal Processe is activated sludge process.
Although, there is problems with traditional active sludge biological method technology maturation：(1) it is big to be aerated pool volume, takes up an area
Area is more, and capital expenditure is more；(2) it is difficult to synchronously realize high-efficient denitrification and dephosphorization efficiency；(3) synchronous nitre is difficult under low dissolved oxygen
Change and denitrification；(4) activated sludge bulking and settling property are unstable；(5) the problems such as capacity of resisting impact load is poor.In order to promote
Enter the progress of process for town sewage treatment, and meet more strict discharge standard, it is necessary to town sewage novel process work
Skill is explored.
Immobilized algal-bacteria syntaxial system is a kind of sewage treatment process of resource-conserving, synchronously can be removed in sewage
The materials such as carbon, nitrogen, phosphorus, and denitrogenation dephosphorizing efficiency high, immobilization algae, bacterium are easily recycled, and one kind is provided for urban wastewater treatment
New approaches.
It is related to more algae, bacteria immobilization technology in presently relevant research and invention and immobilization is mainly formed using investment
The mode of bead, the algae for immobilization are not the preponderant algae identified in actual sewage yet.Such as Deng Xu is published in《Environment
Science》The 8th phase of volume 32 in 2011《The COD and nitrogen phosphorus in sewage are synchronously removed using immobilized algal-bacteria coupled system》Using
Calcium alginate is fixation support, the use of algae is Chlamydomonas reinhardtii；Mao Shuduan etc. is published in《Environmental project journal》2012 the 6th
Rolled up for the 5th phase《Waste water nutrition compares immobilized algal-bacteria and removes pollutant effects and dynamics research》Using calcium alginate as fixation
Change carrier, the use of algae is microalgae；Chinese Patent Application No. 200710168917.2, application publication date are September in 2008 10,
Invention and created name couples the denitrification and dephosphorization method and device of membrane separating process, described frustule immobilization for immobilized algal-bacteria
Material is one kind or any combination in carragheen, calcium alginate, polyvinyl alcohol or polyacrylamide, the use of algae is chlamydomonas,
Chlorella or its combination.Sewage is handled using chemical substance used in above-mentioned document or patent as fixation support, is unfavorable for fully
Algae, the advantage of bacterium mutualism are played, carrier dissolution phenomena easily occurs, causes treatment effect not ideal enough, and cost is higher,
Some problems be present in practice.
The content of the invention
The present invention is intended to provide a kind of bio-denitrifying sewage phosphorus removing method for being easy to engineering application, to solve in fixation
Phycomycete immobilization process complexity be present during change phycomycete processing town sewage, carrier easily dissolves, processing cost is higher, engineering
The shortcomings that changing operational difficulties, technical support is provided for the energy-saving and emission-reduction of urban wastewater treatment.
In order to realize this purpose, the technical scheme is that：
A kind of efficiently immobilized algal-bacteria syntaxial system is to the method for town sewage denitrogenation dephosphorizing, its step：
(1) using PCR-denaturing gradient gel electrophoresis method to urban wastewater treatment firm dirt organism in water
Group is identified, using the algae kind identified as algae source, using activated sludge as bacterium source；
(2) 0.2~0.4g/L of the initial total biological dry weight concentration of control, algae, bacterium is optimal by wastewater treatment efficiency
Dry weight ratio is added in reaction vessel and algae, bacterium is fixed on elastic solid filler；
(3) light source is added to reaction vessel, it is 12h: 12h to control brightness time ratio, 4000~6000lx of intensity of illumination；
(4) simulation sewage or urban wastewater treatment firm sewage, 2~3d of sewage hydraulic detention time are added to reaction unit.
Further, the Oedogonium filamentous algae of step (1) algae source preferably readily apposition growth；
Further, immobilization Oedogonium filamentous algae selection short strip sheath algae.
Further, the optimal algae dry weight of step (2) wastewater treatment efficiency：Bacterium dry weight scope is 1: 5~5
∶1。
Further, step (2) phycomycete immobilization way is stirred or shaken by using to the water body containing algae, bacterium
Water body flow is set to reach algae, bacterium adheres to fixed growth on elastic solid filler.
Further, the addition of step (3) light source is by arranging light source in transparent reaction container surroundings or leading to light source
Cross in transparent hollow post intercalation reaction container.
The advantage of the invention is that：
(1) this algaebacteria symbiotic system has more preferable removal effect to sewage compared to pure algae, pure bacterium.In suitable phycomycete dry weight matter
Under the conditions of amount ratio, the ammonia nitrogen (NH in town sewage after processing4- N), total nitrogen (TN), total phosphorus (TP), COD (CODCr)、
Solid suspension (SS) index reaches the one-level A marks in urban wastewater treatment firm pollutant emission standard (GB18918-2002)
It is accurate.
(2) the algae kind for immobilization is advantageous to from the advantage algae kind short strip sheath algae identified from actual sewage treatment plant
Improve nitrogen phosphorus ligands efficiency in sewage.The algae is filamentous algae, is easily attached on filler, and carrier is provided for bacterium apposition growth, because
It is easy to implement and process for fixation cost is cheap.
(3) phycomycete immobilization processing sewage, phycomycete not easily run off, and ensure processing effluent quality, and are advantageous to algae, bacterium life
The recovery of material, the recycling for biomass energy after processing provide condition.
(4) during this method processing waste water, the advantage of phycomycete mutualism is given full play to, realizes synchronous remove in sewage
Carbon, nitrogen, phosphorus, and be still applicable when influent COD cr concentration is low, engineering is good with prospect.
Embodiment
Embodiment 1
From certain secondary sedimentation tank of sewage treatment work water sampling, according to PCR-denaturing gradient gel electrophoresis (PCR-
DGGE) molecular biology method is analyzed the biocoene in sewage, is as a result shown, preponderant algae in sewage be present：Sheath
Trentepohlia filamentous algae --- short strip sheath algae (Oedogonium brevicingulatum) and asymmetric chlamydomonas (Chlamydomonas
asymmetrica)。
Two kinds of algae immobilization effects are evaluated：4,2L beakers are taken, elastic solid filler is loaded in each beaker, fills out
Expect volumetric filling ratio 60%, two beakers are separately added into the short strip sheath algae of 0.5g dry weights thereto, divide in another two beaker
Not Jia Ru 0.5g dry weights asymmetric chlamydomonas, each beaker adds 1.5L running water, beaker is placed in shaking table, controls water
Rate of flow of fluid 30-60cm/s, intensity of illumination 4000lx, brightness time ratio each beaker taking-up water sample after being 12h: 12h, 24 hours
1000ml, measure wherein algae content.Pass through measurement result：Short strip sheath algae immobilization effect is substantially better than asymmetric chlamydomonas,
Short strip sheath algae fixed rate reaches 99%~100%, and asymmetric chlamydomonas fixed rate is only 2%~5%.Therefore preferred short strip sheath algae
For the algae source of immobilized algal-bacteria system.
Short strip sheath algae dry weight determines that method is：With one group of different volumes of the quantitative filter paper sifting weighing dried in advance
Short strip sheath algae, first weigh and determine this group of short strip sheath algae weight in wet base quality respectively, then this group of algae is placed in 103 ± 1 DEG C of baking ovens and dried
Roasting, drying repeatedly weighs, and determines dry weight, and using algae weight in wet base as abscissa, dry weight is that ordinate draws short strip sheath algae dry and wet weight
Affinity criterions curve.According to standard curve, the short strip sheath algae of certain weight in wet base quality can be converted into dry weight.
Bacterium dry weight determines method：100mL activated sludge accurately is measured, is filtered with weighed quantitative filter paper is dried in advance,
The activated sludge after filtering is together placed in 103 ± 1 DEG C of baking ovens with filter paper again and toasted, drying repeatedly weighs, and determines its dry weight,
Thus bacterium dry weight can be calculated.
2L glass beakers 6 are taken, are placed on six magnetic stirring apparatus, fill 15~20cm elastic solid fillers, volume is filled out
Rate 50%~80% is filled, common 25 watts of fluorescent tubes 2~3,4000~6000lx of intensity of illumination, brightness time are arranged around beaker
Than 12h: 12h, initial total biological dry weight concentration 0.3g/L, according to pure bacterium, algae dry weight：Bacterium dry weight is 1: 5,1:
1st, 5: 1,10: 1 and six experimental groups of pure algae add corresponding algae, bacterium, 2L simulation sewage is added in each beaker, wherein containing
NH4-N：20 ± 0.5mg/L, TP：4 ± 0.5mg/L, CODCr：250 ± 5mg/L, pH：7.58 water temperature：25 ± 5 DEG C, overall process magnetic
Power stirs, and rotor speed control precipitates in beaker bottom without algae and bacterium.With NH4-N、TN、TP、CODCr, pH, SS be monitoring index,
Through 3d processing, algae dry weight：Bacterium dry weight scope is 1: 5~5: 1 experimental group, NH in the simulation sewage after processing4-N、
TN、TP、CODCr, pH, SS reach urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A standards.
Water quality and urban wastewater treatment firm pollutant in embodiment 1 after the initial content of each material, the processing of each experimental group
The data comparison of discharge standard (GB18918-2002) one-level A standards is as follows：
Note：SS initial contents are the phycomycete dry weight concentration initially added, and it is 0 to simulate SS in sewage.
Embodiment 2
The identification of advantage algae, the screening of algae source, the determination of phycomycete dry weight and the setting of experimental group in sewage in the present embodiment
Same as Example 1, each beaker adds simulation sewage quality NH4-N：12 ± 0.5mg/L, TP：2 ± 0.2mg/L, CODCr：
250 ± 5mg/L, pH：7.44 water temperature：25±5℃.Through 3d processing, algae dry weight：Bacterium dry weight scope is 1: 5~5: 1
Experimental group, NH in the simulation sewage after processing4-N、TP、CODCr, pH, SS reach urban wastewater treatment firm pollutant emission
Standard (GB18918-2002) one-level A standards.
Water quality and urban wastewater treatment firm after the initial content of each material in above-described embodiment, the processing of each experimental group is dirty
The data comparison of thing discharge standard (GB18918-2002) the one-level A standards of dye is as follows：
Note：SS initial contents are the phycomycete dry weight concentration initially added, and it is 0 to simulate SS in sewage.
It can be drawn by embodiment 1 and the SS data in embodiment 2, the immobilized algal-bacteria handles the method for sewage, phycomycete
Immobilization effect is good.From NH4-N、TN、TP、CODCrData can show that helotisn has more than single pure algae, pure bacterium to sewage
Good treatment effect, and in algae dry weight：Bacterium dry weight is in the range of 1: 5~5: 1, and the immobilized algal-bacteria system is to simulation
After sewage carries out 3d processing, NH in sewage4-N、TN、TP、CODCr, pH, SS reach urban wastewater treatment firm pollutant emission mark
Accurate (GB18918-2002) one-level A standards.
Embodiment 3
The identification of advantage algae, the screening of algae source and phycomycete dry weight determine same as Example 1, control in sewage in the present embodiment
Initial total biomass 0.3g/L is made, by algae dry weight：Bacterium dry weight sets experimental groups, elastic stereo for 1: 5,1: 1,5: 1
Packing volume filling rate 50%~80%, control intensity of illumination 4000-6000lx, the brightness time than 12h: 12h, with certain a sewage
Treatment plant's water inlet is used as experimental subjects, overall process magnetic agitation, and rotor speed control precipitates in beaker bottom without algae and bacterium.By
2d processing, test NH in water4-N、TN、TP、CODCr, pH, SS reach urban wastewater treatment firm pollutant emission standard
(GB18918-2002) one-level A standards.
Water quality, corresponding clearance and urban wastewater treatment firm after the waste water quality that is used in above-described embodiment, processing is dirty
The data comparison of thing discharge standard (GB18918-2002) the one-level A standards of dye is as follows：
Note：SS is content in urban wastewater treatment firm water inlet.
Embodiment 4
In the present embodiment in sewage advantage algae identification, algae source screening and phycomycete dry weight determine it is same as Example 1, with
For certain b sewage treatment plant inflow as experimental subjects, remaining embodiment is same as Example 3, by 3d processing, tests in water
NH4-N、TN、TP、CODCr, pH, SS value reach urban wastewater treatment firm pollutant emission standard (GB18918-2002) one-level A
Standard.
Water quality, corresponding clearance and urban wastewater treatment firm after the waste water quality that is used in above-described embodiment, processing is dirty
The data comparison of thing discharge standard (GB18918-2002) the one-level A standards of dye is as follows：
Note：SS is content in urban wastewater treatment firm water inlet.
By embodiment 3, embodiment 4 as can be seen that in low concentration CODCrUnder the conditions of this method to town sewage denitrogenation
Phosphor-removing effect is good, the NH in sewage after processing4-N、TN、TP、CODCr, pH, SS index reach one-level A standards, sewage disposal
Journey and required condition are simple, and engineer applied exploitativeness is strong.
Claims (1)
- A kind of 1. efficiently method of the immobilized algal-bacteria syntaxial system to town sewage denitrogenation dephosphorizing, it is characterised in that：(1) using PCR-denaturing gradient gel electrophoresis method to biocoene in urban wastewater treatment firm sewage Identified, using the algae kind identified as algae source, using activated sludge as bacterium source；(2) beaker is placed on six magnetic stirring apparatus, filling 15~20cm elastic solid fillers, volumetric filling ratio 50%~ 80%, according to phycomycete dry weight than 1: 5~5: 1, the Oedogonium short strip filamentous algae and activated sludge for being easy to apposition growth are added, Control 0.2~0.4g/L of initial total biological dry weight concentration, is stirred or shakes to water body containing phycomycete, make water body flow, 30~60cm/s of water volume flow rate, control and precipitate appearance without phycomycete in beaker bottom, phycomycete is adhered to fixed growth in elastic stereo On filler；(3) arrange common fluorescent tube in transparent reaction container surroundings or common fluorescent tube is passed through into transparent hollow post intercalation reaction container In, light source is added to reaction vessel, it is 12h: 12h to control brightness time ratio, 4000~6000lx of intensity of illumination；(4) simulation sewage or urban wastewater treatment firm sewage, 2~3d of sewage hydraulic detention time are added to reaction unit.
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| CN104891678B (en) * | 2015-06-26 | 2016-09-07 | 内蒙古阜丰生物科技有限公司 | For administering the biologic product of the high-concentration waste water that amino acid fermentation produces |
| CN105174476B (en) * | 2015-09-28 | 2017-08-01 | 中国科学院城市环境研究所 | Activated sludge and microalgae coupling particle system and its structure and operation method for wastewater treatment |
| CN105776732A (en) * | 2016-02-19 | 2016-07-20 | 浙江清环保工程有限公司 | Enhanced nitrogen and phosphorus removal device and technology for sewage |
| CN106277582A (en) * | 2016-08-19 | 2017-01-04 | 福建禹环境科技有限公司 | A kind of utilize microalgae method of denitrogenation dephosphorizing in aquaculture wastewater |
| CN108059247A (en) * | 2017-12-31 | 2018-05-22 | 温州大学 | A kind of sewage treatment unit |
| CN108163983B (en) * | 2018-01-17 | 2020-11-10 | 杭州富阳佳畅机械有限公司 | Method for removing nitrogen and phosphorus pollutants by using composite algae preparation |
| CN108623008A (en) * | 2018-04-24 | 2018-10-09 | 武汉大学 | The method that microalgae-aerobic sludge granular syntaxial system is quickly formed under natural light |
| CN109234167B (en) * | 2018-10-10 | 2021-01-15 | 中国农业大学 | Chlorella and application thereof in biogas slurry purification |
| CN109942091B (en) * | 2019-04-09 | 2022-04-29 | 哈尔滨学院 | Bacterial-algae attached biological fiber bed, preparation method thereof and method for strengthening treatment of pig wastewater for nitrogen and phosphorus removal |
| CN110272123A (en) * | 2019-07-03 | 2019-09-24 | 湘潭大学 | A method of immobilized algal-bacteria syntaxial system denitrogenation dephosphorizing efficiency is improved with inorganic carbon source |
| CN113979509B (en) * | 2021-11-05 | 2023-11-10 | 北京农学院 | Application of ultrathin sheet metal hydroxide in antibiotic degradation |
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