CN108163983A - A kind of method using compound algae preparation removal nitrogen and phosphorus pollutants - Google Patents
A kind of method using compound algae preparation removal nitrogen and phosphorus pollutants Download PDFInfo
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- CN108163983A CN108163983A CN201810042034.5A CN201810042034A CN108163983A CN 108163983 A CN108163983 A CN 108163983A CN 201810042034 A CN201810042034 A CN 201810042034A CN 108163983 A CN108163983 A CN 108163983A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/18—PO4-P
Abstract
The invention belongs to environmental technology fields, disclose a kind of method using compound algae preparation removal nitrogen and phosphorus pollutants, include the following steps：By compound algae preparation according to（50‑100g）：The ratio of 1 ton of water body is inoculated in the water body containing nitrogen and phosphorus pollutants, controlled at 26 28 DEG C, Light To Dark Ratio 12：12, intensity of illumination is 5000 10000lux, and processing time is 6 10 days, and liquid is discharged by filter screen, and collects trapped substance recycling.The method of the present invention can effectively remove nitrogen and phosphorus pollutants, simple and feasible, sustainable use.
Description
Technical field
The invention belongs to environmental technology fields, and in particular to a kind of side using compound algae preparation removal nitrogen and phosphorus pollutants
Method.
Background technology
Due to nitrogen and phosphorus element content be more than biological growth demand and caused by water quality deterioration phenomenon be known as nitrogen phosphorus dirt
Dye.Nitrogen and phosphorus pollutants are widely present in sewage.In most of sewage containing needed for a large amount of algal growns nitrogen, phosphorus and its
His nutriment, sewage disposal with the pilot scale culture of microalgae is combined, sewage can not only have been made to reach inexpensive discharge, but also
The cost of microdisk electrode can be saved, while also contains many high added value products in the biomass of microalgae.Have at present
Some reports about algae treated sewage, " Wei Chenggen, suspended alga handle the experimental study of micro-polluted water, 2008 " research
Pollutant removal during suspended alga is discharged Urban River Water, Urban ponds water body and secondary municipal wastewater treatment plant is special
Property；" silver-colored beautiful face etc., the research of immobilized algae Nitrogen/Phosphorus Removal, 2009 " algal immobilization is had studied to manually preparing sewage
The removal effect of middle nitrogen phosphorus.There is good development prospect and advantage using microalgae treated sewage, but sewage middle and high concentration
The factors such as ammonia nitrogen and relatively low transparency are unfavorable for the growth of microalgae, are often difficult to reach preferable using single microalgae treated sewage
Effect.Therefore, developing a kind of method using compound algae preparation removal nitrogen and phosphorus pollutants is particularly important.
Invention content
Present invention aims to solve the deficiencies of the prior art, and provides a kind of a kind of nitrogen and phosphorus pollutants are removed using compound algae preparation
Method by the synergistic effect of a variety of algae, can effectively remove nitrogen and phosphorus pollutants, and compound algae preparation can recycle weight
It is multiple to utilize.
The present invention is achieved by the following technical solution：
A kind of method using compound algae preparation removal nitrogen and phosphorus pollutants includes the following steps：
By compound algae preparation according to（50-100g）：The ratio of 1 ton of sewage is inoculated in the water body containing nitrogen and phosphorus pollutants, control
Temperature is 26-28 DEG C, Light To Dark Ratio 12：12, intensity of illumination 5000-10000lux, processing time is 6-10 days, by filtering
Net discharge liquid, and collect trapped substance reuse.
Specifically,
The compound algae preparation includes Scenedesmus quadricauda, curved rod bar algae and small Closterium.
Specifically,
The compound algae preparation is prepared according to following technique：
1）It takes the logarithm the Scenedesmus quadricauda in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 2 × 107Cell/mL obtains scenedesmus obliquus concentrate；
2）It takes the logarithm the curved rod bar algae in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 1 × 107Cell/mL obtains curved rod bar algae concentrate；
3）It takes the logarithm the small Closterium in growth period, 7000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 3 × 107Cell/mL obtains small Closterium concentrate；
4）By Scenedesmus quadricauda concentrate, curved rod bar algae concentrate and small Closterium concentrate according to 2-3:3-5:The volume of 3-5
Than being mixed to get mixing algae solution, then add in and account for the sodium alginate aqueous solution of mixing algae solution half volume, account for mixing algae solution
The urea of 2% parts by weight and the sodium bicarbonate for accounting for mixing 4% parts by weight of algae solution, stir evenly, then are added dropwise and account for mixing 2 times of bodies of algae solution
Long-pending calcium chloride water shakes when being added dropwise, and after being added dropwise, stands 8h, the algae microballoon of a diameter of 3mm is made.
Preferably,
The preparation method of the sodium alginate aqueous solution is：40g sodium alginates are added to the appearance equipped with 1000ml deionized waters
In device, water bath with thermostatic control, and dissolving is stirred continuously with glass bar, abundant dissolved sodium alginate aqueous solution is put into high pressure sterilization
Pot in sterilize to get.
Preferably,
The preparation method of the calcium chloride water is：20g calcium chloride is added in the container equipped with 1000ml deionized waters,
Stir evenly, then sterilize in high-pressure sterilizing pot to get.
Algae of the present invention can be obtained by conventional cultural method needed for concentration algae solution, this and non-present invention
Innovative point, as space is limited, do not repeat one by one.
The advantageous effect that the present invention obtains mainly includes but is not limited to several aspects：
The method of the present invention can effectively remove nitrogen and phosphorus pollutants, and compound algae preparation can by the synergistic effect of a variety of algae
To recycle and reuse；
The compound algae preparation of the present invention immobilizes processing using three kinds of algae, the space including frustule to be positioned to restriction
Region makes the algae of immobilization that can keep metabolic activity itself and recycle after successive reaction to recycle；
Scenedesmus quadricauda, curved rod bar algae and the small Closterium that the present invention uses are mutually promoted collaboration, can effectively remove the dirt of nitrogen phosphorus
Contaminate object；
After fixation, growth and physiological property change algae of the present invention, improve the absorption to substances such as nitrogen phosphorus and enrichment
Efficiency；
Immobilized microspheres of the present invention are used in combination using a variety of pore-foaming agents, and the pore mechanism of different pore-foaming agents is different, compared to
The effect of single pore-foaming agent, the obtained pore structure size reasonable of the present invention, is evenly distributed, can ensure the quantity of pore structure；
Preparation is simple for the compound algae preparation of the present invention, and cost is more cheap, has a extensive future.
Figure of description
Fig. 1：The compound algae preparation of different compatibilities is to the treatment effect of nitrogen and phosphorus pollutants；
Fig. 2：Influence of the different disposal time to nitrogen and phosphorus pollutants removal effect.
Specific embodiment
Those skilled in the art can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.The product and method of the present invention is described by preferred embodiment, and related personnel can significantly not depart from this hair
Product as described herein and method are modified or suitably changed with combining in bright content, spirit and scope, to realize and answer
Use the technology of the present invention.For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Embodiment 1
A kind of method using compound algae preparation removal nitrogen and phosphorus pollutants includes the following steps：
By compound algae preparation according to 50：The ratio of 1 ton of sewage is inoculated in the water body containing nitrogen and phosphorus pollutants, controlled at 28
DEG C, Light To Dark Ratio 12：12, intensity of illumination 6000lux, processing time are 8 days, and liquid is discharged, and collect and cut by filter screen
Stay object reuse.
The compound algae preparation includes Scenedesmus quadricauda, curved rod bar algae and small Closterium.
Specifically, the compound algae preparation is prepared according to following technique：
1）It takes the logarithm the Scenedesmus quadricauda in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 2 × 107Cell/mL obtains scenedesmus obliquus concentrate；
2）It takes the logarithm the curved rod bar algae in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 1 × 107Cell/mL obtains curved rod bar algae concentrate；
3）It takes the logarithm the small Closterium in growth period, 7000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 3 × 107Cell/mL obtains small Closterium concentrate；
4）By Scenedesmus quadricauda concentrate, curved rod bar algae concentrate and small Closterium concentrate according to 3:5:5 volume ratio mixing
Mixing algae solution is obtained, is then added in and is accounted for the sodium alginate aqueous solution of mixing algae solution half volume, accounts for mixing 2% weight of algae solution
The urea of part and the sodium bicarbonate for accounting for mixing 4% parts by weight of algae solution, stir evenly, then the chlorine for accounting for mixing 2 times of volumes of algae solution is added dropwise
Change calcium aqueous solution, shaken when being added dropwise, after being added dropwise, stand 8h, the algae microballoon of a diameter of 3mm is made.
The preparation method of the sodium alginate aqueous solution is：40g sodium alginates are added to equipped with 1000ml deionized waters
Container in, abundant dissolved sodium alginate aqueous solution is put into high pressure by water bath with thermostatic control, and be stirred continuously dissolving with glass bar
Sterilize in autoclave to get.
The preparation method of the calcium chloride water is：20g calcium chloride is added to the appearance equipped with 1000ml deionized waters
In device, stir evenly, then sterilize in high-pressure sterilizing pot to get.
Embodiment 2
A kind of method using compound algae preparation removal nitrogen and phosphorus pollutants includes the following steps：
By compound algae preparation according to 100g：The ratio of 1 ton of sewage is inoculated in the water body containing nitrogen and phosphorus pollutants, controlled at
28 DEG C, Light To Dark Ratio 12：12, intensity of illumination 8000lux, processing time are 7 days, and liquid is discharged, and collect by filter screen
Trapped substance reuse.
The compound algae preparation includes Scenedesmus quadricauda, curved rod bar algae and small Closterium.
Specifically, the compound algae preparation is prepared according to following technique：
1）It takes the logarithm the Scenedesmus quadricauda in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 2 × 107Cell/mL obtains scenedesmus obliquus concentrate；
2）It takes the logarithm the curved rod bar algae in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 1 × 107Cell/mL obtains curved rod bar algae concentrate；
3）It takes the logarithm the small Closterium in growth period, 7000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 3 × 107Cell/mL obtains small Closterium concentrate；
4）By Scenedesmus quadricauda concentrate, curved rod bar algae concentrate and small Closterium concentrate according to 2:3:3 volume ratio mixing
Mixing algae solution is obtained, is then added in and is accounted for the sodium alginate aqueous solution of mixing algae solution half volume, accounts for mixing 2% weight of algae solution
The urea of part and the sodium bicarbonate for accounting for mixing 4% parts by weight of algae solution, stir evenly, then the chlorine for accounting for mixing 2 times of volumes of algae solution is added dropwise
Change calcium aqueous solution, shaken when being added dropwise, after being added dropwise, stand 8h, the algae microballoon of a diameter of 3mm is made.
The preparation method of the sodium alginate aqueous solution is：40g sodium alginates are added to equipped with 1000ml deionized waters
Container in, abundant dissolved sodium alginate aqueous solution is put into high pressure by water bath with thermostatic control, and be stirred continuously dissolving with glass bar
Sterilize in autoclave to get.
The preparation method of the calcium chloride water is：20g calcium chloride is added to the appearance equipped with 1000ml deionized waters
In device, stir evenly, then sterilize in high-pressure sterilizing pot to get.
Embodiment 3
Present invention processing nitrogen and phosphorus pollutants experiment：
Sample to be tested is selected as the sewage containing nitrogen phosphorus, wherein, NH3-N 211mg/L, TP 82mg/L.
Using embodiment 1 as experimental group, and control group is set, wherein, control group 1：Scenedesmus quadricauda is not added, remaining is same
Embodiment 1；Control group 2：Curved rod bar algae is not added, remaining is the same as embodiment 1；Control group 3：Small Closterium is not added, remaining is the same as real
Apply example 1.As shown in Figure 1, after the compound algae preparation processing of experimental group, control group 1-3, the nitrogen and phosphorus pollutants water in sewage
Flat to substantially reduce, the ability of experimental group processing pollutant is substantially better than control group 1-3, and wherein NH3-N drops respectively than control group 1-3
Low 58.5%, 64.8%, 70.3%；TP reduces by 58.3%, 74.5%, 66.3% than control group 1-3 respectively；Illustrate the compound algae system of experimental group
Three kinds of algae compatibilities that agent uses are reasonable, have preferable collaboration facilitation, and the degradation for being conducive to nitrogen and phosphorus pollutants utilizes；Such as
Shown in Fig. 2, with the increase of processing time, pollutant is substantially reduced, and nitrogen and phosphorus pollutants reached best reduction amount at 8 days,
As the time increases, pollutant level is not substantially reduced, is increased instead, it may be possible to because phosphorus and nitrogen cannot
Meet the needs of high density algae growth, cause part algae dead so that water body is deteriorated.
Embodiment 4
Pore creating material of the present invention is to influence of the compound algae preparation to nitrogen and phosphorus pollutants treatment effect：
Compound algae preparation selection：Embodiment 2 is experimental group；Control group 1：Urea is not added, remaining is the same as embodiment 2；Control group 2：
Sodium bicarbonate is not added, remaining is the same as embodiment 2.Specifically it is shown in Table 1：
Table 1
| Group | Experimental group（mg/L） | Control group 1（mg/L） | Control group 2（mg/L） |
| NH3-N | 13.3 | 20.9 | 16.4 |
| TP | 6.2 | 11.3 | 13.8 |
Conclusion：As shown in table 1, compared with using the control group 1 of single pore creating material and control group 2, experimental group degradation NH3-N and SS
Effect it is more preferable because the addition of two kinds of substances of urea and sodium bicarbonate causes pore structure size reasonable, be distributed more uniform, energy
Enough ensure the quantity of pore structure, improve the adhesive rate of algae, contribute to proliferation and the enrichment of algae, and profit can be recycled again
With reducing cost, have a extensive future.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that：It still may be used
To modify to the technical solution recorded in previous embodiment or carry out equivalent replacement to which part technical characteristic；And
These modifications are replaced, the spirit and model of technical solution of the embodiment of the present invention that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (5)
1. a kind of method using compound algae preparation removal nitrogen and phosphorus pollutants includes the following steps：
By compound algae preparation according to（50-100g）：The ratio of 1 ton of water body is inoculated in the water body containing nitrogen and phosphorus pollutants, control
Temperature is 26-28 DEG C, Light To Dark Ratio 12：12, intensity of illumination 5000-10000lux, processing time is 6-10 days, by filtering
Net discharge liquid, and collect trapped substance recycling.
2. according to the method described in claim 1, it is characterized in that, the compound algae preparation includes Scenedesmus quadricauda, curved rod bar algae
And small Closterium.
3. method according to claim 1 or 2, which is characterized in that the compound algae preparation prepared according to following technique and
：
1）It takes the logarithm the Scenedesmus quadricauda in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 2 × 107Cell/mL obtains scenedesmus obliquus concentrate；
2）It takes the logarithm the curved rod bar algae in growth period, 5000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 1 × 107Cell/mL obtains curved rod bar algae concentrate；
3）It takes the logarithm the small Closterium in growth period, 7000rpm centrifugation 10min remove supernatant, then add sterile water, adjust
The density of frustule is 3 × 107Cell/mL obtains small Closterium concentrate；
4）By Scenedesmus quadricauda concentrate, curved rod bar algae concentrate and small Closterium concentrate according to 2-3:3-5:The volume of 3-5
Than being mixed to get mixing algae solution, then add in and account for the sodium alginate aqueous solution of mixing algae solution half volume, account for mixing algae solution
The urea of 2% parts by weight and the sodium bicarbonate for accounting for mixing 4% parts by weight of algae solution, stir evenly, then are added dropwise and account for mixing 2 times of bodies of algae solution
Long-pending calcium chloride water shakes when being added dropwise, and after being added dropwise, stands 8h, the algae microballoon of a diameter of 3mm is made.
4. according to the method described in claim 3, it is characterized in that, the preparation method of the sodium alginate aqueous solution is：By 40g
Sodium alginate is added in the container equipped with 1000ml deionized waters, water bath with thermostatic control, and is stirred continuously dissolving with glass bar, will fill
Point dissolved sodium alginate aqueous solution be put into high-pressure sterilizing pot sterilize to get.
5. according to the method described in claim 3, it is characterized in that, the preparation method of the calcium chloride water is：By 20g chlorine
Change calcium be added in the container equipped with 1000ml deionized waters, stir evenly, then sterilize in high-pressure sterilizing pot to get.
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Cited By (3)
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| CN107988079A (en) * | 2018-01-17 | 2018-05-04 | 陈英 | A kind of compound algae preparation for removing nitrogen and phosphorus pollutants |
| CN109437409A (en) * | 2018-11-14 | 2019-03-08 | 浙江海洋大学 | A kind of compound microalgae of advanced nitrogen dephosphorization and its preparation method and application |
| CN109985607A (en) * | 2019-04-18 | 2019-07-09 | 中冶华天工程技术有限公司 | A kind of Microcystis aeruginosa ball or micro-capsule algal biscuit for sewage treatment |
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Effective date of registration: 20230828 Address after: Room 401, Unit 1, Building 6, No. 2878 Tuanjie Road, Qingdao Pilot Free Trade Zone, Shandong Province, 266000, China Patentee after: Shandong Wozhisen Ecological Environment Co.,Ltd. Address before: Room 201, Gate 1, Building 5, Huaqing Homeland, Cuitong Road, Dongpuwa Street, Wuqing District, Tianjin 301700 Patentee before: Jiang Liping |
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