CN101811775B - Composite bacteria-algae preparation method for purifying aquaculture waste water - Google Patents
Composite bacteria-algae preparation method for purifying aquaculture waste water Download PDFInfo
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- CN101811775B CN101811775B CN2009100773564A CN200910077356A CN101811775B CN 101811775 B CN101811775 B CN 101811775B CN 2009100773564 A CN2009100773564 A CN 2009100773564A CN 200910077356 A CN200910077356 A CN 200910077356A CN 101811775 B CN101811775 B CN 101811775B
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Abstract
The invention discloses a composite bacteria-algae preparation, which consists of nitrifying bacteria, bacillus licheniformis, scenedesmus obliqnus and selenastrum capricornutum, wherein the volume ratio of the nitrifying bacteria to the bacillus licheniformis to the scenedesmus obliqnus to the selenastrum capricornutum is 1-2: 1-3:1-3:1-3, and the microbe inoculation amount per milliliter is 8*106CFU. The composite bacteria-algae preparation also can be prepared into a globular immobilized preparation by adopting a known embedding method, using the nitrifying bacteria, the bacillus licheniformis, the scenedesmus obliqnus and the selenastrum capricornutum as embedding bacteria and using polyvinyl alcohol, sodium alginate, active carbon, silicon dioxide and calcium carbonate as embedding agents. The bacteria, algae and immobilization materials used by the composite bacteria-algae preparation have no toxic or side effect on aquaculture animals, and can effectively purify the aquaculture waste water; the bacteria and the algae can inhibit the growth of harmful pathogenic bacteria to a certain degree; an immobilization method has simple operation; the immobilized efficient bacteria-algae microbial bactericide is easy to store and transport; and the immobilization materials are economic and practical and easy to recycle.
Description
Technical field
The invention belongs to environmental protection field water treatment category, relate to a kind of composite bacteria-algae preparation that is used for purifying aquaculture waste water particularly.
Technical background
Along with developing rapidly of culture fishery, water pollution is more and more serious, and water quality deterioration has become the principal element of restriction aquaculture development.At present, the research that utilizes mikrobe to carry out water conditioning is new the water conditioning technology and the method for rising in recent decades, existing at home and abroad more report.Mainly concentrate in the processing to water quality such as yeast in research algae, bacterium and the fungi; And with multiple bacterium algae according to optimum proportion, the report that utilizes best process for fixation to be fixed in treating water aquaculture waste on the suitable carriers is not seen as yet.
Bacterium and algae are the important microbe of regulating water quality in the aquatic attitude system; They can effectively control noxious such as ammonia-state nitrogen, nitrite nitrogen content in the aquaculture water; Improve water quality, and have have no side effect, characteristics free from environmental pollution, meet the requirement of healthy aquaculture.
Algae is synthesis of organic substance through inorganic nutrient salt such as absorbed nitrogen, phosphorus, and release of oxygen towards periphery; Bacterium can be decomposed the frustule that utilizes secreted organic substance of algae and death, and its degradation production is absorbed by algae.
The algae immobilization technology originates in the eighties in 20th century, and being mainly used in the biochemical production and the energy in early days provides.In environmental area, immobilized algae is mainly used in biological monitoring and wastewater treatment, and it has the cell density height, and speed of response is fast, advantages such as efficient bacterial strain are run off less, need not separate, can purify and preserve in stable and reliable operation, mikrobe.
Summary of the invention
The object of the present invention is to provide a kind of composite bacteria-algae preparation that is used for purifying aquaculture waste water.Adopt composite bacteria-algae preparation of the present invention can not produce secondary pollution, to cultivated animals toxicological harmless effect, speed of response is fast, stable and reliable operation, and mikrobe is run off few, need not separate.
For realizing above-mentioned purpose; Composite bacteria-algae preparation provided by the invention; Form by nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and crescent moon algae; Wherein, the volume ratio of nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and crescent moon algae is 1-2: 1-3: 1-3: 1-3, and every milliliter of microbial inoculant amount is 7 * 10
6-9 * 10
6CFU.
Composite bacteria-algae preparation of the present invention; Can also adopt known entrapping method; With nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and crescent moon algae is the embedding bacterium; With Z 150PH, sodium-alginate, gac, silicon-dioxide and lime carbonate is that embedding medium is processed globular immobilization preparation, the embedding bacterium: the volume ratio of embedding medium is 1: 2-4; The amount of the Z 150PH in the embedding medium, sodium-alginate, gac, silicon-dioxide and lime carbonate is respectively 70-90g/L, 3-5g/L, 9-11g/L, 7-9g/L, 0.4-0.6g/L.
Characteristics of the present invention are:
1) the treatment process cost of aquiculture waste water is high at present, the current vector is big, operation inconvenience.The present invention is based on bacterium algae physiological and ecological characteristic and confirm bacterium algae kind and allocation ratio, keep the bacterium algae to stablize symbiosis, make up stable microbial ecosystem, make the bacterium algae continue the repair of performance culture environment of aquatic products.
2) the bacterium algae mixing solutions with best proportioning utilizes entrapping method to fix; The microbial metabolism activity is constant, can not produce secondary pollution,, stable and reliable operation fast to cultivated animals toxicological harmless effect, speed of response, mikrobe and run off less, need not separate, can purify and preserve efficient bacterial strain.
Embodiment
Embodiment 1
1) bacterial classification confirms
(a) when inoculum size all be 8 * 10
6During CFU/mL; Screening efficient degradation organism in yeast, nitrobacteria, Bacillus licheniformis, subtilis, photosynthetic bacterium and scenedesmus obliquus, Chlorella pyrenoidesa, crescent moon algae, spiral anabena; And the bacterial classification and the algae strain of ability denitrogenation dephosphorizing, confirm that finally the purpose mikrobe is nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and crescent moon algae.
(b) when inoculum size all be 2 * 10
6During CFU/mL, utilize orthogonal test to confirm the best proportioning between nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and the crescent moon algae, to reach the purpose of better purifying aquaculture waste water.When the lichens genus bacillus: nitrobacteria: crescent moon algae: the volume ratio of scenedesmus obliquus is 1: 2: 2: in the time of 2, add and mix the waste water of bacterium algae solution-treated after 168 hours and all reach water quality standard for fishery (GB11607-89).Through verification experimental verification, mixed bacterium algae solution is more effective than single strain, algae solution-treated aquiculture waste water.Its efficient of removing organism, ammonia-state nitrogen, nitrite nitrogen, nitric nitrogen and dissolved phosphorus is respectively 44.05%, 89.16%, 100%, 98.62%, 100%.
(c) compare the treatment effect that entrapping method and absorption method are fixed the Black Liquor with Efficient Bacteria algae microbiobacterial agent purifying aquaculture waste water of best proportioning
Adopt known entrapping method; Nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and four kinds of embedding bacterium of crescent moon algae algae are processed globular immobilization preparation, and embedding medium is: Z 150PH 80g/L, sodium-alginate 4g/L; Gac 10g/L, silicon-dioxide 8g/L and lime carbonate 0.5g/L.Embedding bacterium algae: the embedding medium volume ratio is 1: 4.Insert 20g immobilization preparation in every 150mL waste water.
Sorbing material is selected pottery gravel, sponge, exsiccant active sludge, and the microbial biomass of every kind of sorbing material access is all identical with entrapping method.Through parallel test, finally adopt fixedly bacterium algae microbiobacterial agent of entrapping method.Immobilized spherule is dropped in the fish jar, and ANOMALOUS VARIATIONS does not take place in cultivated animals, and four kinds of bacterium, the biological total amounts of algae rise on the contrary.
In testing laboratory's fishbowl. in natural lighting, artificial ventilation's condition lower-glass fish jar, put 30 tail tilapias in a suitable place to breed, add artificial diet every day.When water-quality guideline is: DO-7.34; COD
Cr-280.91mg/L; NH
4 +-N-11.44mg/L; NO
2 --N-9.86mg/L; NO
3 --N-16.61mg/L; During DP-2.83mg/L, will prepare and activatory immobilization preparation is added in the fish jar, after 168 hours; The clearance of the organism of water body, ammonia-state nitrogen, nitrite nitrogen, nitric nitrogen and dissolved phosphorus is respectively 44.05%, 89.16%, and 100%; 98.62%; 100%, the waste water after the processing all reaches water quality standard for fishery (GB11607-89), and ANOMALOUS VARIATIONS does not appear in tilapia.
Claims (3)
1. composite bacteria-algae preparation that is used for purifying aquaculture waste water; Form by nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and crescent moon algae; Wherein, The volume ratio of nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and crescent moon algae is 1-2: 1-3: 1-3: 1-3, and every milliliter of microbial inoculant amount is 7 * 10
6-9 * 10
6CFU.
2. composite bacteria-algae preparation as claimed in claim 1, wherein, this composite bacteria-algae preparation is the immobilization preparation.
3. composite bacteria-algae preparation as claimed in claim 2; Wherein, This immobilization preparation is to be the embedding bacterium with nitrobacteria, Bacillus licheniformis, scenedesmus obliquus and crescent moon algae; Z 150PH, sodium-alginate, gac, silicon-dioxide and lime carbonate are embedding medium, the embedding bacterium: the volume ratio of embedding medium is 1: 2-4; Wherein, the amount of the Z 150PH in the embedding medium, sodium-alginate, gac, silicon-dioxide and lime carbonate is respectively 70-90g/L, 3-5g/L, 9-11g/L, 7-9g/L, 0.4-0.6g/L.
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| CN2009100773564A CN101811775B (en) | 2009-02-19 | 2009-02-19 | Composite bacteria-algae preparation method for purifying aquaculture waste water |
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| CN2009100773564A CN101811775B (en) | 2009-02-19 | 2009-02-19 | Composite bacteria-algae preparation method for purifying aquaculture waste water |
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| CN101811775A CN101811775A (en) | 2010-08-25 |
| CN101811775B true CN101811775B (en) | 2012-01-25 |
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| CN101974434B (en) * | 2010-11-11 | 2013-03-27 | 唐清池 | Multi-bacterium microbial compound preparations for aquaculture and preparation method thereof |
| CN102442726B (en) * | 2011-10-26 | 2014-02-26 | 南昌大学 | Fungi-mediated microalgae immobilization wastewater treatment method |
| CN103205412B (en) * | 2013-04-25 | 2016-04-13 | 西华大学 | The method of immobilized microorganism preparation and employing said preparation process meat product processing waste water |
| CN105036344A (en) * | 2015-07-08 | 2015-11-11 | 莆田市岛之星水产有限公司 | Method for biologically purifying aquaculture water body |
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| CN105254019A (en) * | 2015-11-12 | 2016-01-20 | 蚌埠市水森林生态农业科技园 | Formula for treating waste water for aquaculture |
| CN106085997A (en) * | 2016-06-07 | 2016-11-09 | 中国水产科学研究院渔业机械仪器研究所 | A kind of immobilized algal-bacteria ball and its preparation method and application |
| CN106542651A (en) * | 2016-12-20 | 2017-03-29 | 大连赛姆生物工程技术有限公司 | A kind of microorganism water treatment agent in culturing water and preparation method thereof |
| CN107324504B (en) * | 2017-08-09 | 2019-10-08 | 中国科学院水生生物研究所 | A kind of compound algae microbial inoculum and preparation method thereof for culture-pool water quality improvement |
| CN108862605A (en) * | 2018-06-28 | 2018-11-23 | 普定县干河农业开发有限公司 | A kind of application of alga-bacterium symbiosis system pollutant in removal Pig raising wastewater |
| CN108975521A (en) * | 2018-09-07 | 2018-12-11 | 浙江九田环保科技有限公司 | The microbial bacteria agent prescription of aquaculture processing waste water |
| CN109395699A (en) * | 2018-11-14 | 2019-03-01 | 浙江海洋大学 | A method of waste water is handled using microalgae and recycles phosphorus element |
| CN109534513B (en) * | 2018-12-28 | 2022-05-10 | 厦门理工学院 | Method for purifying aquaculture wastewater in situ by biochar-enhanced bacteria-algae immobilized preparation |
| CN110257363A (en) * | 2019-06-19 | 2019-09-20 | 合肥工业大学 | A kind of preparation method and applications of Immobilized hyphae microballoon |
| CN110627218A (en) * | 2019-09-18 | 2019-12-31 | 湖南南山矿泉水有限公司 | Method for treating wastewater in mineral water production process |
| CN110627217A (en) * | 2019-09-18 | 2019-12-31 | 湖南南山矿泉水有限公司 | Pretreatment method for drinking water |
| CN113215050A (en) * | 2021-05-24 | 2021-08-06 | 湖南藻金环保科技有限公司 | Algae-bacterium symbiotic composite microbial preparation for sewage treatment and preparation method thereof |
| CN115568400B (en) * | 2022-11-01 | 2023-07-28 | 珠江水利委员会珠江水利科学研究院 | Fungus and algae symbiotic planting base and preparation method and application thereof |
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