CN1266054C - Modifying agent for microorganism breeding water and preparing method thereof - Google Patents
Modifying agent for microorganism breeding water and preparing method thereof Download PDFInfo
- Publication number
- CN1266054C CN1266054C CNB021452490A CN02145249A CN1266054C CN 1266054 C CN1266054 C CN 1266054C CN B021452490 A CNB021452490 A CN B021452490A CN 02145249 A CN02145249 A CN 02145249A CN 1266054 C CN1266054 C CN 1266054C
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- microorganism cultivation
- microorganism
- composite
- algae
- aquifer amendment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a modifying agent for microorganism breeding water. The present invention is characterized in that a compound microorganism bacterium agent is composed of a compound bacterium agent and microalgae, and the modifying agent is composed of a compound bacterium strain, the microalgae, a culture medium, treacle, a trace element solution, corn powder and a solidifying material. A preparation method of the modifying agent for the microorganism breeding water is characterized in that all used bacterium strains are screened from aquiculture water and substrate sludge. All bacterium strains are bred in the same culture medium by a bacterium strain compound breeding method. A compound curing method of the compound bacterium agent and the microalgae is used. The present invention has the advantage that the modifying agent for the microorganism breeding water can decompose a large amount of residual bait feed, excretion, animal and plant remains accumulated at the bottom of a breeding water area and harmful gas, purifies the water and enhances a survival rate of a breeding animal.
Description
Technical field
The present invention relates to a kind of microorganism cultivation aquifer amendment and making method, can be used for aquaculture, purify water, field such as environmental protection, belong to the aquaculture system improvement
Technical field.
Background technology
In the culture fishery, culture a large amount of remaining bait of bottom, waters accumulation, drain refuse, plant and animal residues and obnoxious flavour, cause easily that water quality is ruined, pathogenic bacteria breeds in a large number and the aquaculture thing is poisoned.
Summary of the invention
The objective of the invention is to invent a kind of microorganism cultivation aquifer amendment and making method that can improve aquaculture system.
For realizing above purpose, technical scheme of the present invention provides a kind of microorganism cultivation aquifer amendment, it is characterized in that, the complex micro organism fungicide of being made up of composite fungus agent and little algae: it is formulated by following weight percentages:
Composite bacteria 5-30%
Little algae 30-70%
Molasses 5-30%
Trace element solution 0.5-15%.
Described composite bacteria is one of Rhodopseudomonas palustris, nitrobacteria, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, actinomyces, streptomyces, bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria or their mixture; Described little algae is one of the lucky egg capsule algae of ripple, Nannochloropsis oceanica or their mixture.
Making method of the present invention is characterized in that, all bacterial classifications of employing and little algae all are to filter out from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum; Adopt composite fungus agent and little algae composite curing method; It comprises the following steps:
(1) strain screening and little algae from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface;
(2) be to cultivate in one of molasses, peptone, sodium-chlor, ferric sulfate, sal epsom, growth factor solution or their mixture all bacterial classifications at same medium component, the ph value is adjusted to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 70-75 hour, obtains composite fungus agent;
(3) composite fungus agent is added inserted in the closed tin fermentation behind little algae, molasses, the trace element solution uniform mixing 24-48 hour, temperature is 25-30 ℃, obtains claims 1 described microorganism cultivation aquifer amendment;
(4) the microorganism cultivation aquifer amendment that makes is added to put into diameter after molasses, Semen Maydis powder uniformly mix be that the solidify material of 5mm stirs with rotating speed 60 commentaries on classics/min, under illumination condition, cultivated 48-72 hour, obtain the microorganism cultivation aquifer amendment of composite curing, the ratio of wherein said microorganism cultivation aquifer amendment, molasses, Semen Maydis powder, solidify material is 30%, 20%, 30%, 20%.
The composite bacteria that strain screening is cultivated from the water body of aquaculture and bed mud, it is rich in monomeric protein, amino acid, organized enzyme, polyphenoils, VITAMIN and other various physiologically active substances, has oxidation, ammonification, nitrated, phosphorus decomposing, sulfuration and solid ammonia effect, objectionable impurities can be decomposed into sulfurous gas, nitrate etc., not only purify water, and can provide nutrition for the breeding of unicellular algae, the photosynthesis of these algae, produce the breathing of cultivated animals again for pool inner water, organic decomposition provides oxygen, thereby form a good ecological circulation, help the growth of aquaculture thing immunological competence and the quickening of the speed of growth, and little algae has decomposing organic matter, increase dissolved oxygen, the ability of absorb polluted matter matter, composite fungus agent has harmful algae with the compound microorganism cultivation aquifer amendment that obtains of little algae and directly subtracts effect extremely, and it can make the algae wall of harmful algae break and cause the frond death of directly turning to be yellow.
Advantage of the present invention is:
1. make polypeptide key in the bacterium protein of harmful bacterium in the water body, virus decompose fracture, with loss of function and dead;
2. organic pollutants such as movement of residual bait and hydrocoles in the decomposition aquaculture water, in and nitrite, the toxicity of materials such as manganese, copper, mercury in the degradation water, control is harmful to the formation of water body and promotes the useful vegeto-animal growth and breeding that swims in the muddy muddy water body, thereby water conditioning is being fit to the benign state that the aquaculture thing is grown up healthy and sound;
3. increase the food consumption of aquaculture thing, significantly improve surviving rate and efficiency of feed utilization, thereby reduce feeding cost, improve and culture efficient;
4. effectively prevent aquatic products material culture disease to take place, strengthen aquaculture thing pipe intestinal digesting absorptive function;
5. can remove peculiar smell and the effect of oxygenation is arranged, fish and shrimp are not had bad stress reaction;
6. effectively control the generation of the bacteriosis of aquaculture things such as the rotted gill disease of light, seawater fish and shrimp, crab, soft-shelled turtle, tortoise, the frog, shellfish and virus and spread, crab is trembled disease, gonorrhoea disease, peach draw disease etc. that obvious curative effects is arranged;
7. guarantee aquaculture safety, efficient, have no side effect, noresidue, pollution-free.
Embodiment
A kind of making method of microorganism cultivation aquifer amendment of composite curing:
(1). strain screening and little algae from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface take by weighing by following weight proportion:
Rhodopseudomonas palustris 20%, nitrobacteria 5%, plant lactobacillus 10%, streptococcus uberis 10%, saccharomyces cerevisiae 10%, actinomyces 12%, streptomyces 8%, bacillus 15%, methane-oxidizing bacteria 5%, ammonia oxidation bacteria 5%;
Take by weighing substratum by following weight proportion:
Molasses 1%, peptone 0.4%, sodium-chlor 0.2%, ferric sulfate 0.05%, sal epsom 0.02%, growth factor solution 1%, ph value 6.8;
(2). all bacterial classifications are cultivated in same substratum, and culture temperature is that 33 ℃, time are 72 hours, obtains composite fungus agent;
Take by weighing little algae by following weight proportion:
The ancient egg capsule algae 60% of ripple, Nannochloropsis oceanica 40% mix;
Take by weighing composite fungus agent and raw material by following weight proportion:
Composite fungus agent 20%, little algae 60%, molasses 18%, trace element solution 2%;
(3). fermented 36 hours inserting behind composite fungus agent, little algae, molasses, the trace element solution uniform mixing in the sealed can.Temperature is 28 ℃, obtains the microorganism cultivation aquifer amendment of composite fungus agent and little algae.
(4) the microorganism cultivation aquifer amendment that makes is added molasses, Semen Maydis powder and uniformly mixes after, the solidify material of putting into straight warp and be 5mm is that urethane stirs with rotating speed 60 commentaries on classics/min comprising tamanori, zeolite or organic polymer material, under illumination condition, cultivated 60 hours, obtain the microorganism cultivation aquifer amendment of composite curing, the ratio of wherein said microorganism cultivation aquifer amendment, molasses, Semen Maydis powder, solidify material is: 30%, 20%, 30%, 20%.
In use, use the microorganism cultivation aquifer amendment of microorganism cultivation aquifer amendment or composite curing according to different situations:
Using the microorganism cultivation aquifer amendment, should mix 100 times of clear water dilutions, culturing or evenly splashing at Chi Tangzhong the last week of pouring water then, every mu of consumption 1kg splashed once in per ten days, can keep water quality optimum stable; As occur raising the nose above water to breathe and during the phenomenon of the general pool, can directly spray the water surface, when crab occurring and trembling disease, mould pinta and rotted gill disease, but dosage use, as finding crab, shrimp trouble gastroenteritis, when not taking food,, can improving surviving rate with the diluent water surface of splashing.
When using solidified microorganism cultivation aquifer amendment, the microorganism cultivation aquifer amendment of composite curing can be put into water, can be suspended in the water at initial 10 minutes, sink at the bottom of the pool after being partly dissolved, but progressively enter the organic pollution materials in the microorganism and little algae decomposition and inversion water body in the water body in the time of in water body, suppress the growth of harmful algae, decompose mud, residual bait after sinking at the bottom of the pool, remove assorted bacterium.
Claims (5)
1. a microorganism cultivation aquifer amendment is characterized in that, the complex micro organism fungicide of being made up of composite fungus agent and little algae: it is formulated by following weight percentages:
Composite bacteria 5-30%
Little algae 30-70%
Molasses 5-30%
Trace element solution 0.5-15%.
2. microorganism cultivation aquifer amendment according to claim 1, it is characterized in that described composite bacteria is one of Rhodopseudomonas palustris, nitrobacteria, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, actinomyces, streptomyces, bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria or their mixture.
3. microorganism cultivation aquifer amendment according to claim 1 is characterized in that, described little algae is one of the lucky egg capsule algae of ripple, Nannochloropsis oceanica or their mixture.
4. the making method of the microorganism cultivation aquifer amendment of a composite curing is characterized in that, all bacterial classifications of employing and little algae all are to filter out from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum; Adopt composite fungus agent and little algae composite curing method; It comprises the following steps:
(1) strain screening and little algae from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface;
(2) be to cultivate in one of molasses, peptone, sodium-chlor, ferric sulfate, sal epsom, growth factor solution or their mixture all bacterial classifications at same medium component, the ph value is adjusted to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 70-75 hour, obtains composite fungus agent;
(3) composite fungus agent is added inserted in the closed tin fermentation behind little algae, molasses, the trace element solution uniform mixing 24-48 hour, temperature is 25-30 ℃, obtains the described microorganism cultivation aquifer amendment of claim 1;
(4) the microorganism cultivation aquifer amendment that makes is added to put into diameter after molasses, Semen Maydis powder uniformly mix be that the solidify material of 5mm stirs with rotating speed 60 commentaries on classics/min, under illumination condition, cultivated 48-72 hour, obtain the microorganism cultivation aquifer amendment of composite curing, the ratio of wherein said microorganism cultivation aquifer amendment, molasses, Semen Maydis powder, solidify material is 30%, 20%, 30%, 20%.
5. the making method of microorganism cultivation aquifer amendment according to claim 4 is characterized in that, described solidify material is tamanori, zeolite, organic polymer material, and the main component of organic polymer material is a urethane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021452490A CN1266054C (en) | 2002-11-12 | 2002-11-12 | Modifying agent for microorganism breeding water and preparing method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021452490A CN1266054C (en) | 2002-11-12 | 2002-11-12 | Modifying agent for microorganism breeding water and preparing method thereof |
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| CN1500749A CN1500749A (en) | 2004-06-02 |
| CN1266054C true CN1266054C (en) | 2006-07-26 |
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Cited By (1)
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2002
- 2002-11-12 CN CNB021452490A patent/CN1266054C/en not_active Expired - Fee Related
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|---|---|---|---|---|
| CN101811775B (en) * | 2009-02-19 | 2012-01-25 | 中国环境科学研究院 | Composite bacteria-algae preparation method for purifying aquaculture waste water |
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