CN104276664B - A kind of microbial water-purifying agent for steamed crab aquaculture water and preparation method thereof - Google Patents
A kind of microbial water-purifying agent for steamed crab aquaculture water and preparation method thereof Download PDFInfo
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- CN104276664B CN104276664B CN201310572175.5A CN201310572175A CN104276664B CN 104276664 B CN104276664 B CN 104276664B CN 201310572175 A CN201310572175 A CN 201310572175A CN 104276664 B CN104276664 B CN 104276664B
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The invention discloses a kind of microbial water-purifying agent for steamed crab aquaculture water and preparation method thereof.Described preparation method: the magnetic silicon ball of 20-40 weight part surface finish C 8 and C18 alkyl chain is joined in the water of 200-400 weight part; Add 130-180 weight part microorganism reagent: Synechococcus 30-60 part, rhodospirillum 30-50 part, Nitrosococcus 10-30 part, nitrated thorn bacterium 10-30 part, thiobacillus denitrificans 10-20 part, iron bacteria 10-20 part, Beggiatoa 3-5 part, Vitreoscilla 1-3 part, plantlike flagellate 30-70 part, zooflagellate 30-50 part, flat algae 20-50 part, chlorella 20-50 part; Add 60-80 weight part beef extract-peptone nutrient agar again to cultivate.By microbial water-purifying agent of the present invention, drop in steamed crab culturing pool, the object of purifying aquaculture Chi Shui can be reached.
Description
Technical field
The present invention relates to a kind of microbial water-purifying agent and preparation method thereof, particularly relate to a kind of microbial water-purifying agent for steamed crab aquaculture water and preparation method thereof.
Background technology
In recent years; the quick propelling of high-efficiency agriculture; special aquaculture industry is developed swift and violent; the new, special products of famous brands, of extra fine quality aquaculture kinds such as river crab, freshwater shrimp, Procambius clarkii, Macrobrachium rosenbergii, Penaeus vannamei, Trionyx sinensis (Wiegmann), mandarin fish, perch, river Puffer, swamp eel, loach have reached the level of large-scale production, define the feature of self.Steamed crab is also known as mitten crab, river crab, eriocheir sinensis, clear water crab or crab, and delicious flavour, nutritious, being a kind of economic crab, is one of famous and precious fishery products of Chinese tradition.Reach the Zhujiang River in China in the south North gets the Liaohe River, very long shoreline extensively distributes, wherein maximum with ChangJiang River output, mouthfeel is the most delicious, the most famous with Suzhou Yangcheng Lake steamed crab.
Steamed crab is dwelt in freshwater lake river, but breeds in brackish water territory, river mouth; Between annual 6 ~ July, newborn young crab is traced back after river enters fresh water, the bank of swinging in rivers, lake of dwelling.Happiness is burrowed and occupies, or is hidden in chad, pasture and water clump.Mainly by 1 pair of foot of a chela when burrowing.Step only helps out.With waterplant, zoobenthos, organic debris and carcase for food.Catch by the foot of a chela when taking food, then food is delivered to a mouthful limit.When nutritional condition is good, when young crab body weight can reach 50 ~ 70 grams, maximumly reach 150 grams.
In current cultural technique, that throws in steamed crab mainly contains natural feed (duckweed, water peanut, eel grass, wild fishes, spiral shell, freshwater mussel etc.), artificial diet (bean curd, bean dregs, soya-bean cake, wheat etc.) and mixed feed etc., but because feed injected volume is often greater than the food-intake of steamed crab, easily cause deteriorating water quality, if do not implement process to aquaculture water, easily cause the rising of steamed crab sickness rate, survival rate reduction, the problems such as production declining.Conventional settling mode is that certain interval of time carries out changing water treatment, but changing water improves production cost on the one hand, and what also easily cause steamed crab on the other hand is not suitable with symptom.
Summary of the invention
For above shortcomings in existing steamed crab cultural technique, one of technical problem to be solved by this invention is to provide a kind of preparation method of microbial water-purifying agent of steamed crab aquaculture water.
Two of technical problem to be solved by this invention is to provide a kind of microbial water-purifying agent of steamed crab aquaculture water, adopts aforesaid method to be prepared from.
Technical problem to be solved by this invention, is achieved by the following technical solution:
For a preparation method for the microbial water-purifying agent of steamed crab aquaculture water, be made up of following step:
(1) magnetic silicon ball of 20-40 weight part finishing alkyl chain is joined in the water of 200-400 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 130-180 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Synechococcus (Synechococcus) 30-60 part, rhodospirillum (Rhodospirillum) 30-50 part, Nitrosococcus (Nitrosococcus) 10-30 part, nitrated thorn bacterium (Nitrospina) 10-30 part, thiobacillus denitrificans (Thiobacillusdenitrificans) 10-20 part, iron bacteria (Crenothrix) 10-20 part, Beggiatoa (Beggiatoia) 3-5 part, Vitreoscilla (Vitreoscilla) 1-3 part, plantlike flagellate (Phrtomastingina) 30-70 part, zooflagellate (Zoomastigna) 30-50 part, flat algae (TetraselmisChui) 20-50 part, chlorella (Chlorella) 20-50 part,
(3) cultivate 20-30 hour under adding 60-80 weight part beef extract-peptone nutrient agar normal temperature again, the microbial water-purifying agent for steamed crab aquaculture water of the present invention can be obtained.
Preferably,
In step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
The magnetic silicon ball of surface finish C 8 alkyl chain, specifically can adopt the method disclosed in Chinese invention patent CN101185874A embodiment 1 to be prepared.
Further, the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
In step (3), described beef extract-peptone nutrient agar adopts ordinary method formulated by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g and water 100ml.
Present invention also offers a kind of microbial water-purifying agent for steamed crab aquaculture water, be prepared from by aforesaid method.
By the microbial water-purifying agent for steamed crab aquaculture water of the present invention, putting into cultivation has in the pond of steamed crab, and water purification agent can sink to the water-settling interface at the bottom of pond, can reach the object of purifying aquaculture Chi Shui.Because the magnetic Nano material that have employed specific surface area large does carrier, this microbial water-purifying agent has and is easy to precipitation, high, the effect stability of purification efficiency and be easy to the advantages such as process, the online treatment of very applicable steamed crab aquaculture water.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1:
(1) preparation of the magnetic silicon ball of surface finish C 8 and C18 alkyl chain:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
(2) magnetic silicon ball of the above-mentioned surface finish C 8 of 30 weight part and C18 alkyl chain is added in the water of 300 weight parts;
(3) under 5 revolutions per seconds of agitation conditions, add the microorganism reagent of 150 weight parts, described microorganism reagent is made up of following weight parts component: Synechococcus (Synechococcus) 50 parts, rhodospirillum (Rhodospirillum) 35 parts, Nitrosococcus (Nitrosococcus) 25 parts, nitrated thorn bacterium (Nitrospina) 20 parts, thiobacillus denitrificans (Thiobacillusdenitrificans) 10 parts, iron bacteria (Crenothrix) 20 parts, Beggiatoa (Beggiatoia) 4 parts, Vitreoscilla (Vitreoscilla) 2 parts, plantlike flagellate (Phrtomastingina) 60 parts, zooflagellate (Zoomastigna) 40 parts, flat algae (TetraselmisChui) 20 parts, chlorella (Chlorella) 50 parts,
(4) the common beef-protein medium of 70 weight part is added again (by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g, water 100ml adopts industry universal method formulated) cultivate 24 hours under normal temperature, the microbial water-purifying agent for steamed crab aquaculture water can be obtained.
Embodiment 2:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for steamed crab aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g dimethyl-octa base silane is adopted to be coating materials in embodiment 2.
Embodiment 3:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for steamed crab aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g octadecyltriethoxy silane is adopted to be coating materials in embodiment 3.
Test case:
Get four piece sizes and the identical steamed crab breeding pool of water quality (2m × 2m × 1m) is tested, the steamed crab seedling 20 of about 10g is thrown in every block pond.Wherein one piece of microbial water-purifying agent of embodiment 1 of splashing; The microbial water-purifying agent of one piece of embodiment 2 of splashing; The microbial water-purifying agent of one piece of embodiment 3 of splashing; Also have one piece and do not throw in any water purification agent, as blank group.
40 grams of microbial water-purifying agents are splashed respectively in steamed crab breeding pool, after 7 days, water-quality determination is carried out to all steamed crab breeding pools.Water quality measurement according to being GB11607-89 National Standard of the People's Republic of China water quality standard for fishery and NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data as shown in the table.
| Testing index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Contrast pond |
| Dissolved oxygen (DO), mg/L | 7.0 | 6.6 | 6.7 | 4.2 |
| Chemical oxygen demand (COD) (COD), mg/L | 64 | 71 | 73 | 135 |
| Five-day BOD (BOD 5)，mg/L | 12.9 | 14.4 | 14.3 | 17.8 |
| Ammonia nitrogen (NH 3-N)，mg/L | 0.08 | 0.12 | 0.11 | 0.21 |
| Nitrite (NO 2-)，mg/L | 0.24 | 0.33 | 0.32 | 0.53 |
| Hydrogen sulfide (H 2S)，mg/L | 0.26 | 0.31 | 0.31 | 0.35 |
As can be seen from data in table, every water-quality guideline of embodiment 1 is all better than embodiment 2 and embodiment 3, is more obviously better than contrasting pond.
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (4)
1. for a preparation method for the microbial water-purifying agent of steamed crab aquaculture water, it is characterized in that, be made up of following step:
(1) magnetic silicon ball of 20-40 weight part finishing alkyl chain is joined in the water of 200-400 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 130-180 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Synechococcus 30-60 part, rhodospirillum 30-50 part, Nitrosococcus 10-30 part, nitrated thorn bacterium 10-30 part, thiobacillus denitrificans 10-20 part, iron bacteria 10-20 part, Beggiatoa 3-5 part, Vitreoscilla 1-3 part, plantlike flagellate 30-70 part, zooflagellate 30-50 part, flat algae 20-50 part, chlorella 20-50 part;
(3) 20-30 hour is cultivated under adding 60-80 weight part beef extract-peptone nutrient agar normal temperature again.
2. as claimed in claim 1 for the preparation method of the microbial water-purifying agent of steamed crab aquaculture water, it is characterized in that: in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
3. as claimed in claim 2 for the preparation method of the microbial water-purifying agent of steamed crab aquaculture water, it is characterized in that, in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained described surface finish C 8 and C18 alkyl chain.
4. for a microbial water-purifying agent for steamed crab aquaculture water, it is characterized in that: adopt method described in claim 1 or 2 or 3 to be prepared from.
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| CN105123584A (en) * | 2015-09-11 | 2015-12-09 | 上海婉静纺织科技有限公司 | Breeding method for crabs |
| CN105557582A (en) * | 2015-12-24 | 2016-05-11 | 常州王者青城生态观光有限公司 | Green aquaculture method |
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| JP3839136B2 (en) * | 1997-07-02 | 2006-11-01 | 独立行政法人科学技術振興機構 | Microorganism-immobilized magnetic carrier, production method thereof and wastewater treatment method |
| CN1266054C (en) * | 2002-11-12 | 2006-07-26 | 上海创博生态工程有限公司 | Modifying agent for microorganism breeding water and preparing method thereof |
| CN1257113C (en) * | 2003-10-08 | 2006-05-24 | 上海创博生态工程有限公司 | Microbiological ammonia nitrogen aligning agent and method for making the same |
| CN102649936A (en) * | 2012-05-02 | 2012-08-29 | 武汉合缘绿色生物工程有限公司 | Compound microorganism fungicide for improving water quality of culturing water body and preparation method |
| CN103352018B (en) * | 2013-07-19 | 2015-04-22 | 上海创博生态工程有限公司 | Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation |
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Effective date of registration: 20170120 Address after: 519000 Guangdong City, Doumen province wells Longjing Town Road, No. 283, the first floor of the workshop, shop No. 104, No. Patentee after: Zhuhai Kang biological technology Co. Ltd. Address before: 201107 Shanghai city Minhang District Minhang Road 88 Lane No. 1-30 building twenty-second room X111 Patentee before: SHANGHAI KUIYA ENVIRONMENTAL PROTECTION TECHNOLOGY CO., LTD. |
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