CN104291448B - A kind of microbial water-purifying agent for culture of Penaeus vannamei water and preparation method thereof - Google Patents
A kind of microbial water-purifying agent for culture of Penaeus vannamei water and preparation method thereof Download PDFInfo
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- CN104291448B CN104291448B CN201310572173.6A CN201310572173A CN104291448B CN 104291448 B CN104291448 B CN 104291448B CN 201310572173 A CN201310572173 A CN 201310572173A CN 104291448 B CN104291448 B CN 104291448B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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Abstract
The invention discloses a kind of microbial water-purifying agent for culture of Penaeus vannamei water and preparation method thereof.Described preparation method: the magnetic silicon ball of 30-50 weight part surface finish C 8 and C18 alkyl chain is joined in the water of 300-500 weight part; Add 150-200 weight part microorganism reagent: red germ 50-80 part, Rhodobacter 50-80 part, Nitrosomonas 20-40 part, Nitrosospira 20-40 part, Pseudomonas stutzeri 10-30 part, Sphaerotilus natans 20-40 part, thiobacillus 4-8 part, pigtail sulphur bacterium 4-8 part, nematode 50-80 part, quiver body worm 50-80 part, Dunaliella salina 20-40 part, chlorella 20-40 part; Add 70-90 weight part beef extract-peptone nutrient agar again to cultivate.By microbial water-purifying agent of the present invention, drop in leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds, the object of purifying aquaculture Chi Shui can be reached.
Description
Technical field
The present invention relates to a kind of microbial water-purifying agent and preparation method thereof, particularly relate to a kind of microbial water-purifying agent for culture of Penaeus vannamei water and preparation method thereof.
Background technology
In recent years; the quick propelling of high-efficiency agriculture; special aquaculture industry is developed swift and violent; the new, special products of famous brands, of extra fine quality aquaculture kinds such as river crab, freshwater shrimp, Procambius clarkii, Macrobrachium rosenbergii, Penaeus vannamei, Trionyx sinensis (Wiegmann), mandarin fish, perch, river Puffer, swamp eel, loach have reached the level of large-scale production, define the feature of self.Penaeus vannamei (PenaeusvannameiBoone), formal name used at school Litoenaeus vannamei, belongs to Arthropoda, Crustachia, Decapoda, swimming suborder, Penaeidae, Penaeus, is wide temperature eurysalinity torrid zone shrimps.Be commonly called as white limb shrimp, white shrimp, the longest 24cm that reaches of adult, crust is thinner, and normal body colour is shallow Steel Gray, and whole body does not have a speckle.Penaeus vannamei is one of three large shrimps that world today's cultured output is the highest.Penaeus vannamei originates in marine site, South America pacific rim, and Penaeus vannamei propagates fast growth artificially, within 60 days, can reach listing specification; Proper salinity level wide (0-40 ‰), can take the multiple aquaculture model of pure fresh water, brackish water, seawater, all can grow from natural waters to freshwater, thus break region restriction, and tool is high temperature resistant, disease resistance is strong; Feeding habits are mixed, and require low to feedstuff protein, 35% can reach needed for growth.Be the fine quality of " extra large shrimp is light to be supported ", make it cultivate territorial scope and expand.
Penaeus vannamei feeding habits are wide and mix, low to the requirement of food, as long as containing the protein component of 25-30% in bait, get final product normal growth.But under artificial high-density breeding condition, in bait, protein component about 40%, should be beneficial to the quick growth of shrimp.But because feed injected volume is often greater than the food-intake of Penaeus vannamei, feed more than needed can not get effective process and easily causes deteriorating water quality, if do not processed timely aquaculture water, easily cause the rising of Penaeus vannamei sickness rate, survival rate reduction, the problems such as production declining.Conventional settling mode is that certain interval of time carries out changing water treatment, but changing water improves production and handling cost on the one hand, and what also easily cause Penaeus vannamei on the other hand is not suitable with symptom.
Summary of the invention
For above shortcomings in existing culture of Penaeus vannamei technology, one of technical problem to be solved by this invention is to provide a kind of preparation method of microbial water-purifying agent of culture of Penaeus vannamei water.
Two of technical problem to be solved by this invention is to provide a kind of microbial water-purifying agent of culture of Penaeus vannamei water, adopts aforesaid method to be prepared from.
Technical problem to be solved by this invention, is achieved by the following technical solution:
For a preparation method for the microbial water-purifying agent of culture of Penaeus vannamei water, be made up of following step:
(1) magnetic silicon ball of 30-50 weight part finishing alkyl chain is joined in the water of 300-500 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 150-200 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: red germ (Rhodomicrobium) 50-80 part, Rhodobacter (Rhodobacterspheroides) 50-80 part, Nitrosomonas (Nitrosomonas) 20-40 part, Nitrosospira (Nitrosospira) 20-40 part, Pseudomonas stutzeri (Pseudomonasstutzeri) 10-30 part, Sphaerotilus natans (Sphaerotilusnatans) 20-40 part, thiobacillus (Thiobacillus) 4-8 part, pigtail sulphur bacterium (Thioploca) 4-8 part, nematode (nematode) 50-80 part, to quiver body worm (Tubifex) 50-80 part, Dunaliella salina (Dunaliella) 20-40 part, chlorella (Chlorella) 20-40 part,
(3) cultivate 20-30 hour under adding 70-90 weight part beef extract-peptone nutrient agar normal temperature again, the microbial water-purifying agent for culture of Penaeus vannamei water of the present invention can be obtained.
Preferably,
In step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
The magnetic silicon ball of surface finish C 8 alkyl chain, specifically can adopt the method disclosed in Chinese invention patent CN101185874A embodiment 1 to be prepared.
Further, the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
In step (3), described beef extract-peptone nutrient agar adopts ordinary method formulated by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g and water 100ml.
Present invention also offers a kind of microbial water-purifying agent for culture of Penaeus vannamei water, be prepared from by aforesaid method.
By the microbial water-purifying agent for culture of Penaeus vannamei water of the present invention, putting into cultivation has in the pond of Penaeus vannamei, and water purification agent can sink to the water-settling interface at the bottom of pond, can reach the object of purifying aquaculture Chi Shui.Because the magnetic Nano material that have employed specific surface area large does carrier, this microbial water-purifying agent has and is easy to precipitation, high, the effect stability of purification efficiency and be easy to the advantages such as process, the online treatment of very applicable culture of Penaeus vannamei water.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1:
(1) preparation of the magnetic silicon ball of surface finish C 8 and C18 alkyl chain:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
(2) magnetic silicon ball of the above-mentioned surface finish C 8 of 35 weight part and C18 alkyl chain is added in the water of 350 weight parts;
(3) under 6 revolutions per seconds of agitation conditions, add the microorganism reagent of 180 weight parts, described microorganism reagent is made up of following weight parts component: red germ 60 parts, Rhodobacter 60 parts, Nitrosomonas 30 parts, Nitrosospira 30 parts, Pseudomonas stutzeri 30 parts, Sphaerotilus natans 40 parts, thiobacillus 6 parts, pigtail sulphur bacterium 6 parts, nematode 60 parts, the body worm 60 parts that quivers, Dunaliella salina 40 parts, chlorella 20 parts;
(4) the common beef-protein medium of 80 weight part is added again (by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g, water 100ml adopts industry universal method formulated) cultivate 24 hours under normal temperature, the microbial water-purifying agent for culture of Penaeus vannamei water can be obtained.
Embodiment 2:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for culture of Penaeus vannamei water, difference is only that in step (1-4), coating materials is different.0.3g dimethyl-octa base silane is adopted to be coating materials in embodiment 2.
Embodiment 3:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for culture of Penaeus vannamei water, difference is only that in step (1-4), coating materials is different.0.3g octadecyltriethoxy silane is adopted to be coating materials in embodiment 3.
Test case:
Get four piece sizes and the identical culture of Penaeus vannamei pond of water quality (2m × 2m × 1.2m) is tested, the Penaeus vannamei seedling 100 that length is about 1 ~ 1.2cm is joined in every block pond.Wherein one piece of microbial water-purifying agent of embodiment 1 of splashing; The microbial water-purifying agent of one piece of embodiment 2 of splashing; The microbial water-purifying agent of one piece of embodiment 3 of splashing; Also have one piece and do not throw in any water purification agent, as blank group.
40 grams of microbial water-purifying agents are splashed respectively in culture of Penaeus vannamei pond, after 7 days, water-quality determination is carried out to all culture of Penaeus vannamei ponds.Water quality measurement according to being GB11607-89 National Standard of the People's Republic of China water quality standard for fishery and NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data as shown in the table.
| Testing index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Contrast pond |
| Dissolved oxygen (DO), mg/L | 7.6 | 7.1 | 7.2 | 5.0 |
| Chemical oxygen demand (COD) (COD), mg/L | 64 | 79 | 82 | 102 |
| Five-day BOD (BOD 5)，mg/L | 10.1 | 11.3 | 11.5 | 15.8 |
| Ammonia nitrogen (NH 3-N)，mg/L | 0.17 | 0.25 | 0.23 | 0.43 |
| Nitrite (NO 2-)，mg/L | 0.04 | 0.06 | 0.06 | 0.11 |
| Hydrogen sulfide (H 2S)，mg/L | 0.17 | 0.24 | 0.26 | 0.36 |
As can be seen from data in table, every water-quality guideline of embodiment 1 is all better than embodiment 2 and embodiment 3, is more obviously better than contrasting pond.
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (4)
1. for a preparation method for the microbial water-purifying agent of culture of Penaeus vannamei water, it is characterized in that, be made up of following step:
(1) magnetic silicon ball of 30-50 weight part finishing alkyl chain is joined in the water of 300-500 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 150-200 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: red germ 50-80 part, Rhodobacter 50-80 part, Nitrosomonas 20-40 part, Nitrosospira 20-40 part, Pseudomonas stutzeri 10-30 part, Sphaerotilus natans 20-40 part, thiobacillus 4-8 part, pigtail sulphur bacterium 4-8 part, nematode 50-80 part, quiver body worm 50-80 part, Dunaliella salina 20-40 part, chlorella 20-40 part;
(3) 20-30 hour is cultivated under adding 70-90 weight part beef extract-peptone nutrient agar normal temperature again.
2. as claimed in claim 1 for the preparation method of the microbial water-purifying agent of culture of Penaeus vannamei water, it is characterized in that: in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
3. as claimed in claim 2 for the preparation method of the microbial water-purifying agent of culture of Penaeus vannamei water, it is characterized in that, in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained described surface finish C 8 and C18 alkyl chain.
4. for a microbial water-purifying agent for culture of Penaeus vannamei water, it is characterized in that: adopt method according to any one of claim 1-3 to be prepared from.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000139446A (en) * | 1998-09-09 | 2000-05-23 | Shikoku Instrumentation Co Ltd | Microorganism decomposing heavy oil |
| CN101139127A (en) * | 2007-08-06 | 2008-03-12 | 华南师范大学 | Functionalized silicon coating mesoporous magnetic carrier and preparation method and application thereof |
| EP1908824A1 (en) * | 1997-07-02 | 2008-04-09 | Japan Science and Technology Agency | Microorganism-immobilized magnetic carriers, a process for producing the carriers and a method of treating wastewater |
| CN101185874A (en) * | 2007-10-11 | 2008-05-28 | 复旦大学 | Surface finish C8 alkyl chain magnetic silicon ball and preparing method and application thereof |
| CN101215540A (en) * | 2008-01-14 | 2008-07-09 | 浙江省农业科学院 | Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof |
-
2013
- 2013-11-17 CN CN201310572173.6A patent/CN104291448B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1908824A1 (en) * | 1997-07-02 | 2008-04-09 | Japan Science and Technology Agency | Microorganism-immobilized magnetic carriers, a process for producing the carriers and a method of treating wastewater |
| JP2000139446A (en) * | 1998-09-09 | 2000-05-23 | Shikoku Instrumentation Co Ltd | Microorganism decomposing heavy oil |
| CN101139127A (en) * | 2007-08-06 | 2008-03-12 | 华南师范大学 | Functionalized silicon coating mesoporous magnetic carrier and preparation method and application thereof |
| CN101185874A (en) * | 2007-10-11 | 2008-05-28 | 复旦大学 | Surface finish C8 alkyl chain magnetic silicon ball and preparing method and application thereof |
| CN101215540A (en) * | 2008-01-14 | 2008-07-09 | 浙江省农业科学院 | Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof |
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