CN104276669B - A kind of microbial water-purifying agent for loach breeding water and preparation method thereof - Google Patents

A kind of microbial water-purifying agent for loach breeding water and preparation method thereof Download PDF

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CN104276669B
CN104276669B CN201310574441.8A CN201310574441A CN104276669B CN 104276669 B CN104276669 B CN 104276669B CN 201310574441 A CN201310574441 A CN 201310574441A CN 104276669 B CN104276669 B CN 104276669B
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water
add
alkyl chain
weight
purifying agent
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CN104276669A (en
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许银亚
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Jiangmen loy biological science and Technology Co., Ltd.
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SHANGHAI KUIYA ENVIRONMENTAL PROTECTION TECHNOLOGY Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used

Abstract

The invention discloses a kind of microbial water-purifying agent for loach breeding water and preparation method thereof.Described preparation method: the magnetic silicon ball of 30-50 weight part surface finish C 8 and C18 alkyl chain is joined in the water of 300-500 weight part; Add 100-150 weight part microorganism reagent: Rhodopseudomonas palustris 60-80 part, Synechococcus 60-80 part, nitrated coccus 10-30 part, Nitrobacter winogradskyi 20-40 part, Pseudomonas aeruginosa 20-30 part, Paracoccus denitrificans 10-20 part, Beggiatoa 3-5 part, thiobacillus 3-5 part, ciliate 30-60 part, plantlike flagellate 30-60 part, Dunaliella salina 30-50 part, chlorella 30-50 part; Add 60-100 weight part beef extract-peptone nutrient agar again to cultivate.By microbial water-purifying agent of the present invention, drop in loach breeding pond, the object of purifying aquaculture Chi Shui can be reached.

Description

A kind of microbial water-purifying agent for loach breeding water and preparation method thereof
Technical field
The present invention relates to a kind of microbial water-purifying agent and preparation method thereof, particularly relate to a kind of microbial water-purifying agent for loach breeding water and preparation method thereof.
Background technology
In recent years; the quick propelling of high-efficiency agriculture; special aquaculture industry is developed swift and violent; the new, special products of famous brands, of extra fine quality aquaculture kinds such as river crab, freshwater shrimp, Procambius clarkii, Macrobrachium rosenbergii, Penaeus vannamei, Trionyx sinensis (Wiegmann), mandarin fish, perch, river Puffer, swamp eel, loach have reached the level of large-scale production, define the feature of self.
Loach is called as " ginseng in water ", all has the distribution north uncommon but the various places that also distribute in southern china various places.The whole year all can gather, and summer is maximum, and be a kind of fish that nutritive value is very high, it is not identical with other fish, no matter appearance, and the bodily form, life habit is all different, is a kind of special bullhead.Loach likes the bottom inhabiting hydrostatic, often haunts the mud top layer being rich in canebreak bottom lake, pond, irrigation canals and ditches and paddy field, strong to environment-adapting ability.Life water temperature 10-30 DEG C, optimum water temperature is 25-27 DEG C.When water temperature in winter drops to below 5 DEG C, namely pierce 20-30cm in mud and survive the winter depths.Loach is many out preys on planktonic organism, aquatic insect, crustacean, Aquatic Higher Plants chip and algae etc. at night, sometimes also absorbs water-bed humus or body refuse.Namely loach 2 reaches maturity age in winter, starts annual April to breed (water temperature 18 DEG C), lays eggs in the shallow water thick grass of water depth deficiency 30 centimetres, and the ovum grain of output sticks to pasture and water or above the drought grass that do not had by water logging.The prelarva hatched, often disperses life, does not form colony.
Propagate loach artificially, the food species of throwing something and feeding has fly maggot, fish meal, Swine blood meal, dried silkworm chrysalis meal, rice bran, wheat bran, dish cake etc.Because the density of cultivation is considerably beyond natural level, water body cannot process the movement of unnecessary bait and loach, causes water body contaminated, and dissolved oxygen declines, and ammonia nitrogen index raises, and has a negative impact to the growth of loach.In current cultural technique, because enforcement process cannot be carried out to aquaculture water, easily cause the rising of loach sickness rate, survival rate reduction, the problems such as production declining.Conventional settling mode is that certain interval of time carries out changing water treatment, but changing water improves production cost on the one hand, and what also easily cause loach on the other hand is not suitable with symptom.
Summary of the invention
Herein for above shortcomings in existing cultural technique, one of technical problem to be solved by this invention is to provide a kind of preparation method of microbial water-purifying agent of loach breeding water.
Two of technical problem to be solved by this invention is to provide a kind of microbial water-purifying agent of loach breeding water, adopts aforesaid method to be prepared from.
Technical problem to be solved by this invention, is achieved by the following technical solution:
For a preparation method for the microbial water-purifying agent of loach breeding water, be made up of following step:
(1) magnetic silicon ball of 30-50 weight part finishing alkyl chain is joined in the water of 300-500 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 100-150 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Rhodopseudomonas palustris (Rhodopseudanonaspalustris) 60-80 part, Synechococcus (Synechococcus) 60-80 part, nitrated coccus (Nitrococcus) 10-30 part, Nitrobacter winogradskyi (Nitrobacterwinogradskyi) 20-40 part, Pseudomonas aeruginosa (P.Aeruginosa) 20-30 part, Paracoccus denitrificans (Paracoccusdenitrificans) 10-20 part, Beggiatoa (Beggiatoia) 3-5 part, thiobacillus (Thiobacillus) 3-5 part, ciliate (Ciliate) 30-60 part, plantlike flagellate (Phrtomastingina) 30-60 part, Dunaliella salina (Dunaliella) 30-50 part, chlorella (Chlorella) 30-50 part,
(3) cultivate 20-30 hour under adding 60-100 weight part beef extract-peptone nutrient agar normal temperature again, the microbial water-purifying agent for loach breeding water of the present invention can be obtained.
Preferably,
In step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
The magnetic silicon ball of surface finish C 8 alkyl chain, specifically can adopt the method disclosed in Chinese invention patent CN101185874A embodiment 1 to be prepared.
Further, the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used 3o 4magnetic nano-particle: by 1 gram of FeCl 36H 2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe 3o 4magnetic nano-particle;
(1-2) by above-mentioned Fe 3o 4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
In step (3), described beef extract-peptone nutrient agar adopts ordinary method formulated by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g and water 100ml.
Present invention also offers a kind of microbial water-purifying agent for loach breeding water, be prepared from by aforesaid method.
By the microbial water-purifying agent for loach breeding water of the present invention, putting into cultivation has in the pond of loach, and water purification agent can sink to the water-settling interface at the bottom of pond, can reach the object of purifying aquaculture Chi Shui.Because the magnetic Nano material that have employed specific surface area large does carrier, this microbial water-purifying agent has and is easy to precipitation, high, the effect stability of purification efficiency and be easy to the advantages such as process, the online treatment of very applicable loach breeding water.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
embodiment 1:
(1) preparation of the magnetic silicon ball of surface finish C 8 and C18 alkyl chain:
(1-1) water heat transfer Fe is used 3o 4magnetic nano-particle: by 1 gram of FeCl 36H 2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe 3o 4magnetic nano-particle;
(1-2) by above-mentioned Fe 3o 4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
(2) magnetic silicon ball of the above-mentioned surface finish C 8 of 40 weight part and C18 alkyl chain is added in the water of 400 weight parts;
(3) under 5 revolutions per seconds of agitation conditions, add the microorganism reagent of 120 weight parts, described microorganism reagent is made up of following weight parts component: Rhodopseudomonas palustris (Rhodopseudanonaspalustris) 70 parts, Synechococcus (Synechococcus) 70 parts, 20 parts, nitrated coccus (Nitrococcus), Nitrobacter winogradskyi (Nitrobacterwinogradskyi) 20 parts, Pseudomonas aeruginosa (P.Aeruginosa) 30 parts, Paracoccus denitrificans (Paracoccusdenitrificans) 20 parts, Beggiatoa (Beggiatoia) 5 parts, thiobacillus (Thiobacillus) 5 parts, ciliate (Ciliate) 40 parts, plantlike flagellate (Phrtomastingina) 50 parts, Dunaliella salina (Dunaliella) 30 parts, chlorella (Chlorella) 50 parts,
(4) the common beef-protein medium of 80 weight part is added again (by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g, water 100ml adopts industry universal method formulated) cultivate 24 hours under normal temperature, the microbial water-purifying agent for loach breeding water can be obtained.
embodiment 2:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for loach breeding water, difference is only that in step (1-4), coating materials is different.0.3g dimethyl-octa base silane is adopted to be coating materials in embodiment 2.
embodiment 3:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for loach breeding water, difference is only that in step (1-4), coating materials is different.0.3g octadecyltriethoxy silane is adopted to be coating materials in embodiment 3.
test case:
Get four piece sizes and the identical loach breeding pond of water quality (2m × 2m × 1m) is tested, the loach seedling 500 of about 3g is thrown in every block pond.Wherein one piece of microbial water-purifying agent of embodiment 1 of splashing; The microbial water-purifying agent of one piece of embodiment 2 of splashing; The microbial water-purifying agent of one piece of embodiment 3 of splashing; Also have one piece and do not throw in any water purification agent, as blank group.
By microbial water-purifying agent according to 10 grams/meter 3ratio carry out full pool spilling head, after 7 days, water-quality determination is carried out to all loach breeding ponds.Water quality measurement according to being GB11607-89 National Standard of the People's Republic of China water quality standard for fishery and NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data as shown in the table.
Testing index Embodiment 1 Embodiment 2 Embodiment 3 Contrast pond
Dissolved oxygen (DO), mg/L 7.2 6.8 6.7 4.5
Chemical oxygen demand (COD) (COD), mg/L 61 73 72 102
Five-day BOD (BOD 5),mg/L 11.9 12.7 12.6 15.6
Ammonia nitrogen (NH 3-N) ,mg/L 0.15 0.21 0.22 0.30
Nitrite (NO 2~),mg/L 0.24 0.33 0.32 0.61
Hydrogen sulfide (H 2S) ,mg/L 0.13 0.22 0.20 0.35
As can be seen from data in table, every water-quality guideline of embodiment 1 is all better than embodiment 2 and embodiment 3, is more obviously better than contrasting pond.
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.

Claims (4)

1. for a preparation method for the microbial water-purifying agent of loach breeding water, it is characterized in that, be made up of following step:
(1) magnetic silicon ball of 30-50 weight part finishing alkyl chain is joined in the water of 300-500 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 100-150 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Rhodopseudomonas palustris 60-80 part, Synechococcus 60-80 part, nitrated coccus 10-30 part, Nitrobacter winogradskyi 20-40 part, Pseudomonas aeruginosa 20-30 part, Paracoccus denitrificans 10-20 part, Beggiatoa 3-5 part, thiobacillus 3-5 part, ciliate 30-60 part, plantlike flagellate 30-60 part, Dunaliella salina 30-50 part, chlorella 30-50 part;
(3) 20-30 hour is cultivated under adding 60-100 weight part beef extract-peptone nutrient agar normal temperature again.
2. as claimed in claim 1 for the preparation method of the microbial water-purifying agent of loach breeding water, it is characterized in that: in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
3. as claimed in claim 2 for the preparation method of the microbial water-purifying agent of loach breeding water, it is characterized in that, in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used 3o 4magnetic nano-particle: by 1 gram of FeCl 36H 2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe 3o 4magnetic nano-particle;
(1-2) by above-mentioned Fe 3o 4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained described surface finish C 8 and C18 alkyl chain.
4. for a microbial water-purifying agent for loach breeding water, it is characterized in that: adopt method according to any one of claim 1-3 to be prepared from.
CN201310574441.8A 2013-11-17 2013-11-17 A kind of microbial water-purifying agent for loach breeding water and preparation method thereof Active CN104276669B (en)

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CN106337028A (en) * 2015-07-17 2017-01-18 上海海洋大学 Cleaning agent, and preparation method and application thereof
CN105248317A (en) * 2015-09-18 2016-01-20 远睿生物科技有限公司 Method for improving water quality in loach cultivation
CN107864870A (en) * 2017-09-27 2018-04-03 胡征远 A kind of method for cultivating zero-emission

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1778717A (en) * 2004-11-22 2006-05-31 王防修 Water-purifying agent
CN101053827A (en) * 2007-05-10 2007-10-17 复旦大学 Surface fixing metal ions magnetic microspheres and its preparation method and application
CN101215540A (en) * 2008-01-14 2008-07-09 浙江省农业科学院 Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof
CN101935105A (en) * 2010-07-22 2011-01-05 浙江工商大学 Microbial purifying agent for aquaculture water and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1778717A (en) * 2004-11-22 2006-05-31 王防修 Water-purifying agent
CN101053827A (en) * 2007-05-10 2007-10-17 复旦大学 Surface fixing metal ions magnetic microspheres and its preparation method and application
CN101215540A (en) * 2008-01-14 2008-07-09 浙江省农业科学院 Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof
CN101935105A (en) * 2010-07-22 2011-01-05 浙江工商大学 Microbial purifying agent for aquaculture water and preparation method thereof

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