CN104276663B - A kind of microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water and preparation method thereof - Google Patents
A kind of microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water and preparation method thereof Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 49
- 241000736919 Pelodiscus sinensis Species 0.000 title claims abstract description 30
- 238000009360 aquaculture Methods 0.000 title claims abstract description 24
- 230000000813 microbial effect Effects 0.000 title claims abstract description 24
- 239000012629 purifying agent Substances 0.000 title claims abstract description 24
- 244000144974 aquaculture Species 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 19
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 19
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 19
- 239000010703 silicon Substances 0.000 claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- 241001495394 Nitrosospira Species 0.000 claims abstract description 8
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000005864 Sulphur Substances 0.000 claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 7
- 244000005700 microbiome Species 0.000 claims abstract description 7
- 241000223782 Ciliophora Species 0.000 claims abstract description 6
- 241000192700 Cyanobacteria Species 0.000 claims abstract description 6
- 241000862991 Leptothrix <Bacteria> Species 0.000 claims abstract description 6
- 239000001888 Peptone Substances 0.000 claims abstract description 6
- 241001509286 Thiobacillus denitrificans Species 0.000 claims abstract description 6
- 241000195493 Cryptophyta Species 0.000 claims abstract description 4
- 241000223785 Paramecium Species 0.000 claims abstract description 4
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 4
- 235000015278 beef Nutrition 0.000 claims abstract description 4
- 239000006916 nutrient agar Substances 0.000 claims abstract description 4
- 241000684087 bacterium 1-3 Species 0.000 claims abstract 4
- 239000006185 dispersion Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 14
- 239000011248 coating agent Substances 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 239000002122 magnetic nanoparticle Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 7
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 6
- 239000004005 microsphere Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims description 4
- 229910000077 silane Inorganic materials 0.000 claims description 4
- FZMJEGJVKFTGMU-UHFFFAOYSA-N triethoxy(octadecyl)silane Chemical compound CCCCCCCCCCCCCCCCCC[Si](OCC)(OCC)OCC FZMJEGJVKFTGMU-UHFFFAOYSA-N 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical class [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 235000011091 sodium acetates Nutrition 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 abstract 1
- 230000008569 process Effects 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000013505 freshwater Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000190909 Beggiatoa Species 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 241000192497 Oscillatoria Species 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 241000894100 Tetraselmis chuii Species 0.000 description 2
- 241000190807 Thiothrix Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 241000252185 Cobitidae Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000238553 Litopenaeus vannamei Species 0.000 description 1
- 241001327110 Macrobrachium rosenbergii Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000646357 Monopterus cuchia Species 0.000 description 1
- 241000237536 Mytilus edulis Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000269799 Perca fluviatilis Species 0.000 description 1
- 241000404975 Synchiropus splendidus Species 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 235000020638 mussel Nutrition 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Ecology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a kind of microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water and preparation method thereof.Described preparation method: the magnetic silicon ball of 20-40 weight part surface finish C 8 and C18 alkyl chain is joined in the water of 200-400 weight part; Add 80-100 weight part microorganism reagent: Rhodopseudomonas palustris 50-70 part, greatly quiver blue bacterium 50-70 part, Nitrosospira 10-30 part, nitrosification leaf bacterium 10-30 part, thiobacillus denitrificans 10-20 part, leptothrix falciformis 10-20 part, send out sulphur bacterium 1-3 part, bayesian sulphur bacterium 1-3 part, paramecium 30-70 part, ciliate 30-70 part, flat algae 20-40 part, blue-green algae 20-40 part; Add 40-60 weight part beef extract-peptone nutrient agar again to cultivate.By microbial water-purifying agent of the present invention, drop in Trionyx sinensis (Wiegmann) culturing pool, the object of purifying aquaculture Chi Shui can be reached.
Description
Technical field
The present invention relates to a kind of microbial water-purifying agent and preparation method thereof, particularly relate to a kind of microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water and preparation method thereof.
Background technology
In recent years; the quick propelling of high-efficiency agriculture; special aquaculture industry is developed swift and violent; the new, special products of famous brands, of extra fine quality aquaculture kinds such as river crab, freshwater shrimp, Procambius clarkii, Macrobrachium rosenbergii, Penaeus vannamei, Trionyx sinensis (Wiegmann), mandarin fish, perch, river Puffer, swamp eel, loach have reached the level of large-scale production, define the feature of self.Trionyx sinensis (Wiegmann) is a kind of high protein (15 ~ 17%), lower fat (1 ~ 4%) and fishery products containing multivitamin and trace element, edible and pharmaceutical use is all very high, has very high economic worth.Trionyx sinensis (Wiegmann) is based on the food of animality, and propagating artificially under condition, within 12 ~ 15 months, body weight can to about 400 ~ 500g.Feed nutrition: protein accounts for 40 ~ 50% (animal proteinum accounts for more than 80%), and fat accounts for less than 1%, and carbohydrate is no more than 20%.General feed kind has soya bean, cabbage, fishes and shrimps, spiral shell, freshwater mussel and chaff cake etc.But in the process propagated artificially, because the density of cultivation is considerably beyond natural level, water body cannot process the movement of unnecessary bait and Trionyx sinensis (Wiegmann), cause water body contaminated, dissolved oxygen declines, and ammonia nitrogen index raises, and has a negative impact to the growth of Trionyx sinensis (Wiegmann).
In current cultural technique, because enforcement process cannot be carried out to aquaculture water, easily cause the rising of Trionyx sinensis (Wiegmann) sickness rate, survival rate reduction, the problems such as production declining.Conventional settling mode is that certain interval of time carries out changing water treatment, but changing water improves production cost on the one hand, and what also easily cause Trionyx sinensis (Wiegmann) on the other hand is not suitable with symptom.
Summary of the invention
For above shortcomings in existing cultural technique, one of technical problem to be solved by this invention is to provide a kind of preparation method of microbial water-purifying agent of Trionyx sinensis (Wiegmann) aquaculture water.
Two of technical problem to be solved by this invention is to provide a kind of microbial water-purifying agent of Trionyx sinensis (Wiegmann) aquaculture water, adopts aforesaid method to be prepared from.
Technical problem to be solved by this invention, is achieved by the following technical solution:
For a preparation method for the microbial water-purifying agent of Trionyx sinensis (Wiegmann) aquaculture water, be made up of following step:
(1) magnetic silicon ball of 20-40 weight part finishing alkyl chain is joined in the water of 200-400 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 80-100 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Rhodopseudomonas palustris (Rhodopseudanonaspalustris) 50-70 part, to quiver greatly blue bacterium (Oscillatoria) 50-70 part, Nitrosospira (Nitrosospira) 10-30 part, nitrosification leaf bacterium (Nitrosolobus) 10-30 part, thiobacillus denitrificans (Thiobacillusdenitrificans) 10-20 part, leptothrix falciformis (Leptothrix) 10-20 part, send out sulphur bacterium (Thiothrix) 1-3 part, bayesian sulphur bacterium (Beggiatoa) 1-3 part, paramecium (Paramoecium) 30-70 part, ciliate (Ciliate) 30-70 part, flat algae (TetraselmisChui) 20-40 part, blue-green algae (Cyanobacteria) 20-40 part,
(3) cultivate 20-30 hour under adding 40-60 weight part beef extract-peptone nutrient agar normal temperature again, the microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water of the present invention can be obtained.
Preferably,
In step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
The magnetic silicon ball of surface finish C 8 alkyl chain, specifically can adopt the method disclosed in Chinese invention patent CN101185874A embodiment 1 to be prepared.
Further, the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
In step (3), described beef extract-peptone nutrient agar adopts ordinary method formulated by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g and water 100ml.
Present invention also offers a kind of microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water, be prepared from by aforesaid method.
By the microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water of the present invention, putting into cultivation has in the pond of Trionyx sinensis (Wiegmann), and water purification agent can sink to the water-settling interface at the bottom of pond, can reach the object of purifying aquaculture Chi Shui.Because the magnetic Nano material that have employed specific surface area large does carrier, this microbial water-purifying agent has and is easy to precipitation, high, the effect stability of purification efficiency and be easy to the advantages such as process, the online treatment of very applicable Trionyx sinensis (Wiegmann) aquaculture water.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1:
(1) preparation of the magnetic silicon ball of surface finish C 8 and C18 alkyl chain:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
(2) magnetic silicon ball of the above-mentioned surface finish C 8 of 30 weight part and C18 alkyl chain is added in the water of 300 weight parts;
(3) under 5 revolutions per seconds of agitation conditions, add the microorganism reagent of 90 weight parts, described microorganism reagent is made up of following weight parts component: Rhodopseudomonas palustris (Rhodopseudanonaspalustris) 60 parts, quiver greatly blue bacterium (Oscillatoria) 60 parts, Nitrosospira (Nitrosospira) 20 parts, nitrosification leaf bacterium (Nitrosolobus) 20 parts, thiobacillus denitrificans (Thiobacillusdenitrificans) 15 parts, leptothrix falciformis (Leptothrix) 15 parts, send out sulphur bacterium (Thiothrix) 2 parts, bayesian sulphur bacterium (Beggiatoa) 2 parts, paramecium (Paramoecium) 50 parts, ciliate (Ciliate) 50 parts, flat algae (TetraselmisChui) 30 parts, blue-green algae (Cyanobacteria) 30 parts,
(4) the common beef-protein medium of 50 weight part is added again (by extractum carnis 0.5g, peptone 1g, NaCl0.5g, agar 2.0g, water 100ml adopts industry universal method formulated) cultivate 24 hours under normal temperature, the microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water can be obtained.
Embodiment 2:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g dimethyl-octa base silane is adopted to be coating materials in embodiment 2.
Embodiment 3:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g octadecyltriethoxy silane is adopted to be coating materials in embodiment 3.
Test case:
Get four piece sizes and the identical Trionyx sinensis (Wiegmann) breeding pool of water quality (2m × 2m × 1m) is tested, the Trionyx sinensis (Wiegmann) seedling 50 of about 100g is thrown in every block pond.Wherein one piece of microbial water-purifying agent of embodiment 1 of splashing; The microbial water-purifying agent of one piece of embodiment 2 of splashing; The microbial water-purifying agent of one piece of embodiment 3 of splashing; Also have one piece and do not throw in any water purification agent, as blank group.
By microbial water-purifying agent according to 10 grams/meter
3ratio carry out full pool spilling head, after 7 days, water-quality determination is carried out to all Trionyx sinensis (Wiegmann) breeding pools.Water quality measurement according to being GB11607-89 National Standard of the People's Republic of China water quality standard for fishery and NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data as shown in the table.
| Testing index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Contrast pond |
| Dissolved oxygen (DO), mg/L | 7.5 | 6.8 | 6.9 | 3.5 |
| Chemical oxygen demand (COD) (COD), mg/L | 68 | 83 | 82 | 112 |
| Five-day BOD (BOD 5)，mg/L | 11.6 | 14.2 | 13.1 | 17.6 |
| Ammonia nitrogen (NH 3-N)，mg/L | 0.25 | 0.28 | 0.29 | 0.35 |
| Nitrite (NO 2～)，mg/L | 0.34 | 0.53 | 0.62 | 1.21 |
| Hydrogen sulfide (H 2S)，mg/L | 0.23 | 0.32 | 0.30 | 0.45 |
As can be seen from data in table, every water-quality guideline of embodiment 1 is all better than embodiment 2 and embodiment 3, is more obviously better than contrasting pond.
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (4)
1. for a preparation method for the microbial water-purifying agent of Trionyx sinensis (Wiegmann) aquaculture water, it is characterized in that, be made up of following step:
(1) magnetic silicon ball of 20-40 weight part finishing alkyl chain is joined in the water of 200-400 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 80-100 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Rhodopseudomonas palustris 50-70 part, greatly quiver blue bacterium 50-70 part, Nitrosospira 10-30 part, nitrosification leaf bacterium 10-30 part, thiobacillus denitrificans 10-20 part, leptothrix falciformis 10-20 part, send out sulphur bacterium 1-3 part, bayesian sulphur bacterium 1-3 part, paramecium 30-70 part, ciliate 30-70 part, flat algae 20-40 part, blue-green algae 20-40 part;
(3) 20-30 hour is cultivated under adding 40-60 weight part beef extract-peptone nutrient agar normal temperature again.
2. as claimed in claim 1 for the preparation method of the microbial water-purifying agent of Trionyx sinensis (Wiegmann) aquaculture water, it is characterized in that: in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
3. as claimed in claim 2 for the preparation method of the microbial water-purifying agent of Trionyx sinensis (Wiegmann) aquaculture water, it is characterized in that, in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3g, add the ultrasonic 8min of 5ml2MHCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5ml10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5ml water-dispersion;
(1-3) get above-mentioned dispersion liquid B1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained described surface finish C 8 and C18 alkyl chain.
4. for a microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water, it is characterized in that: adopt method according to any one of claim 1-3 to be prepared from.
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