CN104278019A - Microbial water purifying agent for mandarin fish culture water and preparation method thereof - Google Patents
Microbial water purifying agent for mandarin fish culture water and preparation method thereof Download PDFInfo
- Publication number
- CN104278019A CN104278019A CN201310571975.5A CN201310571975A CN104278019A CN 104278019 A CN104278019 A CN 104278019A CN 201310571975 A CN201310571975 A CN 201310571975A CN 104278019 A CN104278019 A CN 104278019A
- Authority
- CN
- China
- Prior art keywords
- water
- parts
- mandarin fish
- add
- microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a microbial water purifying agent for mandarin fish culture water and a preparation method of the agent. The preparation method comprises the following steps: adding 40-50 parts by weight of magnetic silicon spheres of which the surface is modified with C8 and C18 alkyl chains to 200-250 parts by weight of water; adding 100-150 parts by weight of microbial reagents which include 40-60 parts of synechococcus, 40-50 parts of rhodobacter sphaeroides, 30-40 parts of nitrospina, 30-50 parts of nitrococcus, 20-40 parts of sphaerotilus natans, 30-50 parts of pseudomonas aeruginosa, 3-5 parts of leucothrix, 3-5 parts of thioploca, 50-60 parts of amoeba, 30-50 parts of suctorida, 30-40 parts of dunaliella and 30-50 parts of tetraselmis; next, adding 50-80 parts by weight of beef extract peptone agar culture medium for culturing. The microbial water purifying agent is put into a mandarin fish culture pond to purify water in the culture pond.
Description
Technical field
The present invention relates to a kind of microbial water-purifying agent and preparation method thereof, particularly relate to a kind of microbial water-purifying agent for mandarin fish aquaculture water and preparation method thereof.
Background technology
In recent years; the quick propelling of high-efficiency agriculture; special aquaculture industry is developed swift and violent; the new, special products of famous brands, of extra fine quality aquaculture kinds such as river crab, freshwater shrimp, Procambius clarkii, Macrobrachium rosenbergii, Penaeus vannamei, Trionyx sinensis (Wiegmann), mandarin fish, perch, river Puffer, swamp eel, loach have reached the level of large-scale production, define the feature of self.Mandarin fish is again mandarin fish, Ao Huayu, belongs to the fat section fish in taxonomy.Huge legendary turtle flower fish is fish the famousst and precious in " three spend five sieve ".Mandarin fish body is flat, and tripe abdomen is broad, and mouth is large and squama thin, first and tail is short.The bodily form is that the spot of black is color, color clear be male, more black is a little female, and fish back has ray.The skin of mandarin fish is thicker, meat is abundant solid, delicious flavour, rich in proteins, corn is few, can tonifying the five internal organs, beneficial taste, inflate stomach, treat deficient, be the first-class food fish in fresh-water fishes, be applicable to deficient qi and blood physique, the diseases such as consumptive disease is weak, discharging fresh blood stool can be controlled, therefore deeply like by the masses, market recognition is high.
The natural food of mandarin fish is all the little small fish of some economic worths, shrimp and brit.What conventional raising was thrown in is mostly long strip type or spindle-type low value fish, as: dace, silver carp, flathead, grass, silver xenocypris, carp, crucian etc.But because feed injected volume is often greater than the food-intake of mandarin fish, unnecessary feed can not get effective process and easily causes deteriorating water quality, if do not implement process to aquaculture water, easily cause the rising of mandarin fish sickness rate, survival rate reduction, the problems such as production declining.Conventional settling mode is that certain interval of time carries out changing water treatment, but changing water improves production and handling cost on the one hand, and what also easily cause mandarin fish on the other hand is not suitable with symptom.
Summary of the invention
For above shortcomings in existing mandarin fish cultural technique, one of technical problem to be solved by this invention is to provide a kind of preparation method of microbial water-purifying agent of mandarin fish aquaculture water.
Two of technical problem to be solved by this invention is to provide a kind of microbial water-purifying agent of mandarin fish aquaculture water, adopts aforesaid method to be prepared from.
Technical problem to be solved by this invention, is achieved by the following technical solution:
For a preparation method for the microbial water-purifying agent of mandarin fish aquaculture water, be made up of following step:
(1) magnetic silicon ball of 40-50 weight part finishing alkyl chain is joined in the water of 200-250 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 100-150 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Synechococcus (Synechococcus) 40-60 part, Rhodobacter (Rhodobacter spheroides) 40-50 part, nitrated thorn bacterium (Nitrospina) 30-40 part, nitrated coccus (Nitrococcus) 30-50 part, Sphaerotilus natans (Sphaerotilus natans) 20-40 part, Pseudomonas aeruginosa (P.Aeruginosa) 30-50 part, bright bacterium (Luecothrix) 3-5 part, pigtail sulphur bacterium (Thioploca) 3-5 part, amoeba (Sareodina) 50-60 part, worms on a sucker (Suctoria) 30-50 part, Dunaliella salina (Dunal iel la) 30-40 part, flat algae (Tetraselmis Chui) 30-50 part,
(3) cultivate 20-30 hour under adding 50-80 weight part beef extract-peptone nutrient agar normal temperature again, the microbial water-purifying agent for mandarin fish aquaculture water of the present invention can be obtained.
Preferably,
In step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
The magnetic silicon ball of surface finish C 8 alkyl chain, specifically can adopt the method disclosed in Chinese invention patent CN101185874A embodiment 1 to be prepared.
Further, the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3 g, add the ultrasonic 8min of 5ml 2M HCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5 ml 10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5 ml water-dispersions;
(1-3) get above-mentioned dispersion liquid B 1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
In step (3), described beef extract-peptone nutrient agar adopts ordinary method formulated by extractum carnis 0.5g, peptone 1g, NaCl 0.5g, agar 2.0g and water 100 ml.
Present invention also offers a kind of microbial water-purifying agent for mandarin fish aquaculture water, be prepared from by aforesaid method.
By the microbial water-purifying agent for mandarin fish aquaculture water of the present invention, putting into cultivation has in the pond of mandarin fish, and water purification agent can sink to the water-settling interface at the bottom of pond, can reach the object of purifying aquaculture Chi Shui.Because the magnetic Nano material that have employed specific surface area large does carrier, this microbial water-purifying agent has and is easy to precipitation, high, the effect stability of purification efficiency and be easy to the advantages such as process, the online treatment of very applicable mandarin fish aquaculture water.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
embodiment 1:
(1) preparation of the magnetic silicon ball of surface finish C 8 and C18 alkyl chain:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3 g, add the ultrasonic 8min of 5ml 2M HCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5 ml 10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5 ml water-dispersions;
(1-3) get above-mentioned dispersion liquid B 1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained surface finish C 8 and C18 alkyl chain.
(2) magnetic silicon ball of the above-mentioned surface finish C 8 of 40 weight part and C18 alkyl chain is added in the water of 200 weight parts;
(3) under 5 revolutions per seconds of agitation conditions, add the microorganism reagent of 120 weight parts, described microorganism reagent is made up of following weight parts component: Synechococcus (Synechococcus) 40 parts, Rhodobacter (Rhodobacter spheroides) 50 parts, nitrated thorn bacterium (Nitrospina) 40 parts, 40 parts, nitrated coccus (Nitrococcus), Sphaerotilus natans (Sphaerotilus natans) 35 parts, Pseudomonas aeruginosa (P.Aeruginosa) 45 parts, bright bacterium (Luecothrix) 4 parts, pigtail sulphur bacterium (Thioploca) 4 parts, amoeba (Sareodina) 50 parts, worms on a sucker (Suctoria) 30 parts, Dunaliella salina (Dunal iel la) 30 parts, flat algae (Tetraselmis Chui) 30 parts,
(4) the common beef-protein medium of 60 weight part is added again (by extractum carnis 0.5g, peptone 1g, NaCl 0.5g, agar 2.0g, water 100 ml adopts industry universal method formulated) cultivate 24 hours under normal temperature, the microbial water-purifying agent for mandarin fish aquaculture water can be obtained.
embodiment 2:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for mandarin fish aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g dimethyl-octa base silane is adopted to be coating materials in embodiment 2.
embodiment 3:
Substantially the same manner as Example 1, obtain the microbial water-purifying agent for mandarin fish aquaculture water, difference is only that in step (1-4), coating materials is different.0.3g octadecyltriethoxy silane is adopted to be coating materials in embodiment 3.
test case:
(4m × 4m × 1.5 m) are tested, and the mandarin fish seedling 80 that length is 2.5 ~ 3.5 cm is joined in every block pond to get four piece sizes and the identical mandarin fish breeding pool of water quality.Wherein one piece of microbial water-purifying agent of embodiment 1 of splashing; The microbial water-purifying agent of one piece of embodiment 2 of splashing; The microbial water-purifying agent of one piece of embodiment 3 of splashing; Also have one piece and do not throw in any water purification agent, as blank group.
40 grams of microbial water-purifying agents are splashed respectively in mandarin fish breeding pool, after 7 days, water-quality determination is carried out to all mandarin fish breeding pools.Water quality measurement according to being GB11607-89 National Standard of the People's Republic of China water quality standard for fishery and NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data as shown in the table.
| Testing index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Contrast pond |
| Dissolved oxygen (DO), mg/L | 7.2 | 7.0 | 6.8 | 4.9 |
| Chemical oxygen demand (COD) (COD), mg/L | 76 | 85 | 91 | 124 |
| Five-day BOD (BOD 5)，mg/L | 11.5 | 13.7 | 14.3 | 16.7 |
| Ammonia nitrogen (NH 3-N) ，mg/L | 0.11 | 0.14 | 0.15 | 0.24 |
| Nitrite (NO 2-)，mg/L | 0.05 | 0.07 | 0.06 | 0.10 |
| Hydrogen sulfide (H 2S) ，mg/L | 0.15 | 0.22 | 0.20 | 0.35 |
As can be seen from data in table, every water-quality guideline of embodiment 1 is all better than embodiment 2 and embodiment 3, is more obviously better than contrasting pond.
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (4)
1. for a preparation method for the microbial water-purifying agent of mandarin fish aquaculture water, it is characterized in that, be made up of following step:
(1) magnetic silicon ball of 40-50 weight part finishing alkyl chain is joined in the water of 200-250 weight part;
(2) under 2-10 revolutions per second of agitation condition, add 100-150 weight part microorganism reagent, described microorganism reagent is made up of following weight parts component: Synechococcus 40-60 part, Rhodobacter 40-50 part, nitrated thorn bacterium 30-40 part, nitrated coccus 30-50 part, Sphaerotilus natans 20-40 part, Pseudomonas aeruginosa 30-50 part, bright bacterium 3-5 part, pigtail sulphur bacterium 3-5 part, amoeba 50-60 part, worms on a sucker 30-50 part, Dunaliella salina 30-40 part, flat algae 30-50 part;
(3) 20-30 hour is cultivated under adding 50-80 weight part beef extract-peptone nutrient agar normal temperature again.
2. as claimed in claim 1 for the preparation method of the microbial water-purifying agent of mandarin fish aquaculture water, it is characterized in that: in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 alkyl chain and/or the magnetic silicon ball of finishing C18 alkyl chain.
3. as claimed in claim 2 for the preparation method of the microbial water-purifying agent of mandarin fish aquaculture water, it is characterized in that, in step (1), the magnetic silicon ball of described finishing alkyl chain is the magnetic silicon ball of surface finish C 8 and C18 alkyl chain, adopts following method to be prepared from:
(1-1) water heat transfer Fe is used
3o
4magnetic nano-particle: by 1 gram of FeCl
36H
2o, is dissolved in 30ml ethylene glycol, and add 4 grams of anhydrous sodium acetates, temperature of reaction is 200 DEG C, and the reaction times is 12 hours, generates Fe
3o
4magnetic nano-particle;
(1-2) by above-mentioned Fe
3o
4magnetic nano-particle washes with water, and removing water-soluble impurity, is then dispersed in 10g water, obtain dispersion liquid A, get centrifuge tube, add the above-mentioned dispersion liquid A of 3 g, add the ultrasonic 8min of 5ml 2M HCl solution, be separated the green clear liquid of removing with magnet again, then use 10ml water-dispersion, then collect particle with magnet, remove light green clear liquid, with the citric acid three sodium solution ultrasonic disperse of 5 ml 10wt%, leave standstill 2h, again obtain dispersion liquid B with after 5 ml water-dispersions;
(1-3) get above-mentioned dispersion liquid B 1g, after 5ml water and 30ml alcohol dilution, then add 20wt% ammoniacal liquor 0.5ml, ultrasonic disperse 10 minutes, stir, add 1ml tetraethoxy, stirring at room temperature 12 hours, collect with magnet, washing, dry, obtain complex microsphere;
(1-4) above-mentioned for 0.01g complex microsphere is joined in 1.0g anhydrous pyridine, ultrasonic disperse, and then add coating materials, described coating materials is the mixture of 0.1g dimethyl-octa base silane and 0.2g octadecyltriethoxy silane composition, stirring at room temperature 12 hours, with ethanol and water washing, then vacuum-drying, the magnetic silicon ball of obtained described surface finish C 8 and C18 alkyl chain.
4. for a microbial water-purifying agent for mandarin fish aquaculture water, it is characterized in that: adopt method described in claim 1 and 2 and 3 to be prepared from.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310571975.5A CN104278019A (en) | 2013-11-15 | 2013-11-15 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310571975.5A CN104278019A (en) | 2013-11-15 | 2013-11-15 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104278019A true CN104278019A (en) | 2015-01-14 |
Family
ID=52253365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310571975.5A Pending CN104278019A (en) | 2013-11-15 | 2013-11-15 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104278019A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828678A (en) * | 2017-10-17 | 2018-03-23 | 福建师范大学福清分校 | One plant height production manganese oxidase sheath bacteria, biology pure culture, manganese oxidase and application |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185874A (en) * | 2007-10-11 | 2008-05-28 | 复旦大学 | Surface finish C8 alkyl chain magnetic silicon ball and preparing method and application thereof |
| CN102091605A (en) * | 2010-12-01 | 2011-06-15 | 中国科学院生态环境研究中心 | Preparation and application of high-capacity magnetic mesoporous silica gel solid phase extracting agent |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN102765776A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Active biological water purifying agent and preparation method thereof |
| CN102963985A (en) * | 2012-11-30 | 2013-03-13 | 江西鸿吉生物科技有限公司 | Biological preparation for balancing water quality for aquaculture |
| CN103275963A (en) * | 2013-06-27 | 2013-09-04 | 东南大学 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
| CN104276665A (en) * | 2013-11-16 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for snakehead culture water and preparation method thereof |
| CN104278020A (en) * | 2013-11-17 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for weever farming water and preparation method of microbial water purifying agent |
-
2013
- 2013-11-15 CN CN201310571975.5A patent/CN104278019A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101185874A (en) * | 2007-10-11 | 2008-05-28 | 复旦大学 | Surface finish C8 alkyl chain magnetic silicon ball and preparing method and application thereof |
| CN102091605A (en) * | 2010-12-01 | 2011-06-15 | 中国科学院生态环境研究中心 | Preparation and application of high-capacity magnetic mesoporous silica gel solid phase extracting agent |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN102765776A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Active biological water purifying agent and preparation method thereof |
| CN102963985A (en) * | 2012-11-30 | 2013-03-13 | 江西鸿吉生物科技有限公司 | Biological preparation for balancing water quality for aquaculture |
| CN103275963A (en) * | 2013-06-27 | 2013-09-04 | 东南大学 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
| CN104276665A (en) * | 2013-11-16 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for snakehead culture water and preparation method thereof |
| CN104278020A (en) * | 2013-11-17 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for weever farming water and preparation method of microbial water purifying agent |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828678A (en) * | 2017-10-17 | 2018-03-23 | 福建师范大学福清分校 | One plant height production manganese oxidase sheath bacteria, biology pure culture, manganese oxidase and application |
| CN107828678B (en) * | 2017-10-17 | 2021-06-01 | 福建师范大学福清分校 | High-yield manganese oxidase sphingomyelinase, biologically pure culture, manganese oxidase and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103392642B (en) | Method for breeding mandarin fish by utilizing microorganic feed | |
| CN105755088A (en) | Method for inducing haematococcus pluvialis to produce C40H52O4 | |
| CN105393953A (en) | High-yield culture method for lobsters | |
| CN106616030A (en) | Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof | |
| CN106665431A (en) | Breeding method of crispy grass carp | |
| CN113308377A (en) | Microalgae culture medium, culture method thereof and culture water purification method | |
| CN105210953A (en) | The high-yield cultivation method of shrimp | |
| CN109717117A (en) | A kind of warm canopy efficient circulation cultural method of Penaeus Vannmei | |
| CN104276665B (en) | A kind of microbial water-purifying agent for snakeheaded fish aquaculture water and its preparation method | |
| CN104276669B (en) | A kind of microbial water-purifying agent for loach breeding water and preparation method thereof | |
| CN104276667B (en) | A kind of microbial water-purifying agent for grass shrimp aquaculture water and preparation method thereof | |
| CN102599374B (en) | Selenium-enriched kelp feed and application thereof to culture of selenium-enriched abalone | |
| CN104276664B (en) | A kind of microbial water-purifying agent for steamed crab aquaculture water and preparation method thereof | |
| CN105884451A (en) | Culture medium for freshwater shrimp and crab breeding and preparation method of culture medium | |
| CN104278019A (en) | Microbial water purifying agent for mandarin fish culture water and preparation method thereof | |
| CN104278020A (en) | Microbial water purifying agent for weever farming water and preparation method of microbial water purifying agent | |
| CN104276663B (en) | A kind of microbial water-purifying agent for Trionyx sinensis (Wiegmann) aquaculture water and preparation method thereof | |
| Li et al. | Freshwater pearl culture | |
| CN107788211A (en) | Young shrimp resisting stress dousing agent of a kind of Macrobrachium rosenbergii and preparation method thereof | |
| CN104276666B (en) | A kind of microbial water-purifying agent for river Puffer aquaculture water and preparation method thereof | |
| CN104291448B (en) | A kind of microbial water-purifying agent for culture of Penaeus vannamei water and preparation method thereof | |
| CN103535298A (en) | Method for using nanometer slow release feed to breed mandarin fish | |
| CN104276668A (en) | Microbiological water purifying agent for ricefield eel breeding water and preparation method of microbiological water purifying agent | |
| CN104782539A (en) | Disease-resistant freshwater shrimp culturing method | |
| CN111374076A (en) | Penaeus vannamei feeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150114 |