CN103275963A - Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem - Google Patents
Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem Download PDFInfo
- Publication number
- CN103275963A CN103275963A CN2013102648832A CN201310264883A CN103275963A CN 103275963 A CN103275963 A CN 103275963A CN 2013102648832 A CN2013102648832 A CN 2013102648832A CN 201310264883 A CN201310264883 A CN 201310264883A CN 103275963 A CN103275963 A CN 103275963A
- Authority
- CN
- China
- Prior art keywords
- concentration
- preparation
- pva
- immobilized microorganism
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/40—Protecting water resources
- Y02A20/402—River restoration
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a preparation method of an immobilized microorganism embedding microsphere for restoring a riverbed bottom mud ecosystem. The preparation method comprises the steps of PVA (polyvinyl alcohol) gel preparation, cross-linking agent preparation and microsphere preparation, wherein the PVA gel preparation comprises the steps of preparing a PVA solution with a concentration of 8-10%, adding embedding agents to the PVA solution, namely sodium alginate with a concentration of 0.5-2%, calcium carbonate with a concentration of 0.2-0.5%, silicon dioxide with a concentration of 2.0-4.0%, and 300-500 mesh attapulgite powder or 300-500 mesh activated carbon powder with a concentration of 0.5-1.0%, and adding activated limon microorganism bacterium liquid with a concentration of 10-15%; and the microsphere preparation comprises the steps of dropwise adding PVA gel to a calcium chloride saturated boric acid solution, stirring, obtaining immobilized microorganism activated spherular particles with the particle size of 3-5mm, conducting immobilized crosslinking for 24-36h at 4-8 DEG C, taking out, and washing with normal saline. Compared with the prior art, the microsphere can remove total organic carbon and total nitrogen in riverbed bottom mud effectively, and can improve COD (chemical oxygen demand), ammonia nitrogen and total nitrogen pollution conditions of an overlying water body of the bottom mud, and the preparation method is an ecological riverway management scheme which is efficient, low-consumption and simple to operate, and has a development prospect.
Description
Technical field:
The present invention relates to a kind of immobilized microorganism embedding treatment method for riverbed bed mud ecosystem restoration, specifically is the embedding method for preparing microsphere of microorganism embedding treatment method.
Background technology:
Stream pollution is one of ubiquitous outstanding environmental problem in the present global range, important component part as river ecosystem, bed mud is not only the key link of all kinds of material cycle in river, and is the main gathering storehouse of these materials---and bed mud is pollution substance in the water body " storehouse " and " source ".Therefore, research and improvement that bed mud in river pollutes are the important contents of stream pollution comprehensive improvement, are one of important channels that fundamentally solves the stream pollution problem.
The river bottom mud reparation mainly contains the physics reparation, chemistry is repaired and biological restoration three major types recovery technique.The physics reparation comprises dredging and covering etc., and environmental dredging costs consumption power, and easily original ecotope is caused damage, and the water correction effect is very unobvious yet; Soverlay technique can't fundamentally be removed pollutent, uses covering material can reduce lake storage capacity in a large number, and searching covering material source also becomes problem.The chemistry reparation need add chemical agent in water, investment is big, destroys river ecosystem, and is easy to generate secondary pollution.
The immobilized microorganism method is advanced in the world water body treating method, adopts certain technique means (absorption method, entrapping method, crosslinking and blocking cut-off process etc.) to make microorganism set growth, and microorganism is not suspended in water and still keeps biological activity, and can recycle.Entrapping method is that microorganism is fixed in the specific polymer carrier grid structure, utilizes carrier self structure characteristic, makes the small-molecule substance carrier of freely coming in and going out, and with inner microbial interaction, reaches the effect of removing pollutent.Adopt immobilized microorganism technique, can effectively degrade organic wastewater with difficult degradation thereby, heavy metal wastewater thereby and ammonia nitrogen waste water etc.Screening has the microbial population of specific hardly degraded organic substances such as degraded phenol, lipid from environment, and applying immobilized microbial technique is implemented immobilization to it, and degradation efficiency obviously is better than traditional biologic treating technique; A large amount of algae and microbial cells can effectively absorb with enrichment sewage in heavy metal, compare with free suspension algae and thalline, immobilization can reduce heavy metal to the toxic action of algae and thalline, shows great potentiality in handling heavy metal wastewater thereby; Immobilized microorganism technique can form anaerobic zone and aerobic zone simultaneously inside and outside particle, nitration reaction and anti-nitration reaction can be finished in same reactor, can the strengthened denitrification effect, become environmentalist's research focus gradually.
Oneself has been subjected to concern widely the distinct advantages of immobilized microorganism technique aspect water treatment, but its research and application at riverbed bed mud ecosystem restoration almost is blank.After point-source pollution (sewage discharge belongs to point-source pollution) is effectively controlled, bed mud just becomes important endogenous that river water pollutes, a large amount of hazardous contaminants of accumulating in the bed mud, again discharging by certain exchange interaction, is the main secondary pollution source of influence and restriction overlying water matter.How effectively to control the riverbed sediment pollution, improve water quality of river, become most important environmental problem in China's water surrounding.
Summary of the invention:
The present invention carries and will solve to such an extent that technical problem is at above-mentioned the deficiencies in the prior art, and the microorganism embedding method for preparing microsphere that provides the bed mud embedding of a kind of riverbed to administer, can effectively remove total organic carbon and total nitrogen in the riverbed bed mud by microballoon of the present invention, improving bed mud overlying water COD, ammonia nitrogen and total nitrogen pollution situation, is a kind of efficient low-consume, simple to operate, promising river channel ecology resolution.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of preparation method of the immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration, it is characterized in that: step is as follows:
1, preparation PVA gel:
Configuration concentration is 8～10% polyvinyl alcohol solution;
Add embedding medium in above-mentioned polyvinyl alcohol solution: concentration is that 0.5%～2% sodium alginate, the calcium carbonate of concentration 0.2%～0.5%, concentration are that 2.0～4.0% silicon-dioxide and concentration are 0.5～1.0% 300～500 order attapulgite powder or 300～500 order active carbon powders;
The profit that adds concentration and be after 10～15% activation is covered microbial inoculum, and the described sharp microbial inoculum that covers comprises two kinds of S-1 and N-1, and S-1 and N-1 volume ratio are 1:1;
2, linking agent preparation: take by weighing 7.5～8.0g boric acid, 3.5～4.0g Calcium Chloride Powder Anhydrous is dissolved in the 200mL water, is made into the saturated boric acid solution of calcium chloride of desired concn, and regulate pH to 7～8 with yellow soda ash;
3, microballoon prepares: the PVA gel drops that mixes is added in the saturated boric acid solution of calcium chloride stirs, obtaining particle diameter is the active coccoid particle of immobilized microorganism of 3～5mm, is under 4～8 ℃ in temperature then, fixing crosslinked 24～36h, take out, use normal saline flushing.
Described attapulgite concentration is 0.5%, and crosslinking time is 36h, and PVA concentration is 8%, and microbial inoculum concentration is 15%, and calcium carbonate concentration is 0.4%, and silica concentration is 2.5%, and sodium alginate is 0.5%.
Described attapulgite concentration is 0.5%, and crosslinking time is 36h, and microbial inoculum concentration is 15%, and silica concentration is 3.5%, and sodium alginate concentration is 1%, and calcium carbonate concentration is that 0.4%, PVA concentration is 8%.
The polyvinyl alcohol solution of described interpolation embedding medium stirs, and is cooled to 20～25 ℃ of 3～5h.
The sharp illiteracy of described S-1 microbial inoculum is Bacillus subtilus, bacillus amyloliquefaciens, Bacillus licheniformis, fiber pseudomonas bacillus, Pseuomonas denitrifican, Rhodopseudomonas palustris, dinitrogen fiber bacterium or Pseudomonas stutzeri; The sharp illiteracy of N-1 microbial inoculum is Nitrosomonas, nitrosification spirillum, nitrosification leaf bacterium, nitrated thorn bacterium, Vickers bacterium nitrobacter or nitrated coccus.
In the process of the active bead of preparation immobilized microorganism, the factor that influences the immobilized spherule degradation of contaminant mainly contains PVA gel strength, embedding medium concentration, microbial inoculum concentration and crosslinking time.
PVA concentration is low excessively, and pellet hardness is not enough, and difficult forming is broken easily; The PVA excessive concentration, then gel densification strengthens the matrix resistance to mass transfer, and treatment effect is bad.In sum, select the PVA concentration range between 8% and 10%.
In embedding carrier fixation of microbe process, add a small amount of sodium alginate, silicon-dioxide and calcium carbonate in proportion, permeability and the physical strength of PVA particle all are improved to some extent.Add active carbon powder or attapulgite powder, both strengthened the mass transfer ability of biological bead, improved its physical strength again.On the previous experiments basis, choose sodium alginate concentration between 0.5% and 1%, calcium carbonate concentration is between 0.2% and 0.4%, and silica concentration is between 2.5% and 3.5%, between 300 order active carbon powders or 300 order attapulgite powder concentration 0.5% and 1%.
Microbial inoculum concentration has determined the content of microorganism in the immobilized microorganism bead, and the removal of pollution substance is had a direct impact.Microbial inoculum concentration is low excessively, and pollution substance is removed not thorough; The microbial inoculum excessive concentration, microorganism is separated out easily.On the previous experiments basis, choose microbial inoculum concentration between 10% and 15%.
Crosslinking time refers to the molding time of immobilized microorganism bead in saturated boric acid solution.Crosslinking time is too short, the bead insufficient strength; Crosslinking time is long, because boric acid to the toxic effect of microorganism, can reduce cytoactive.On the previous experiments basis, choose crosslinking time between 24h and 36h.
Compared with prior art, microballoon of the present invention can effectively be removed total organic carbon and the total nitrogen in the riverbed bed mud, improves bed mud overlying water COD, ammonia nitrogen and total nitrogen pollution situation, is a kind of efficient low-consume, simple to operate, promising river channel ecology resolution.
Description of drawings
The preparation flow synoptic diagram of the active small spherical particles of Fig. 1 immobilized microorganism;
Fig. 2 overlying water COD concentration changes with time situation synoptic diagram;
Fig. 3 overlying water NH
4 +-N concentration changes with time situation synoptic diagram;
Fig. 4 overlying water NO
2 --N concentration changes with time situation synoptic diagram;
Fig. 5 overlying water NO
3 --N concentration changes with time situation synoptic diagram;
Fig. 6 bed mud TOC concentration changes with time situation synoptic diagram;
Fig. 7 different dissolved oxygen concentration is to the synoptic diagram that influences of ammonia nitrogen, total nitrogen and COD treatment effect;
The different pH values of Fig. 8 are to the synoptic diagram that influences of ammonia nitrogen, total nitrogen and COD treatment effect;
Fig. 9 bed mud total nitrogen concentration is the changing conditions synoptic diagram in time;
Figure 10 bed mud heterotrophic organism and reversal of cure bacterial number be the changing conditions synoptic diagram in time.
Embodiment
Below in conjunction with accompanying drawing the present invention is elaborated:
The preparation method of a kind of immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration of the present invention, its process step is as shown in Figure 1, and is specific as follows:
1, preparation PVA gel:
Configuration concentration is 8～10% polyvinyl alcohol solution: add embedding medium in above-mentioned polyvinyl alcohol solution: concentration is that 0.5%～2% sodium alginate, the calcium carbonate of concentration 0.2%～0.5%, concentration are that 2.0～4.0% silicon-dioxide and concentration are 0.5～1.0% 300～500 order attapulgite powder or 300～500 order active carbon powders; The profit that adds concentration and be after 10～15% activation is covered microbial inoculum, and the described sharp microbial inoculum that covers comprises two kinds of S-1 and N-1, and S-1 and N-1 volume ratio are 1:1;
Specifically can adopt following method configuration: take by weighing 7.5～10.0g polyvinyl alcohol by concentration requirement and add in the 100ml water preferred 1788 types of polyvinyl alcohol.Dissolve fully under 85～90 ℃ with thermostat water bath after soaking into 24h, add a certain amount of embedding medium (sodium alginate 0.5～2.0g then, silicon-dioxide 2.0～4.0g, calcium carbonate 0.～0.5g, 300 order attapulgite powder or 300 order active carbon powders 0.5～1.0g) stir, be cooled to 20～25 ℃ of 3h-5h, adding profit after 10～15% activation covers microbial inoculum (the sharp microbial inoculum that covers comprises two kinds of S-1 and N-1, S-1: Bacillus subtilus, bacillus amyloliquefaciens, Bacillus licheniformis, the fiber pseudomonas bacillus, Pseuomonas denitrifican, Rhodopseudomonas palustris, dinitrogen fiber bacterium, Pseudomonas stutzeri etc.; N-1: Nitrosomonas, nitrosification spirillum, nitrosification leaf bacterium, nitrated thorn bacterium, Vickers bacterium nitrobacter, nitrated coccus etc.S-1 and N-1 volume ratio are 1:1.）
2, linking agent preparation: take by weighing 7.5～8.0g boric acid, 3.5～4.0g Calcium Chloride Powder Anhydrous is dissolved in the 200mL water, is made into the saturated boric acid solution of calcium chloride of desired concn, and regulate pH to 7～8 with yellow soda ash;
3, microballoon preparation: the PVA gel drops that mixes is added in the saturated boric acid solution of calcium chloride, specifically can adopt peristaltic pump to add, and use magnetic stirring apparatus constantly to stir, obtaining particle diameter is the active coccoid particle of immobilized microorganism of 3～5mm, be under 4～8 ℃ in temperature then, fixing crosslinked 24～36h takes out, and uses normal saline flushing.
Be influence factor with calcium carbonate concentration (A), silica concentration (B), active carbon powder or attapulgite powder concentration (C), sodium alginate concentration (D), crosslinking time (E), microbial inoculum concentration (F) and PVA concentration (G), each factor is chosen two levels, adopts L
8(2
7) orthogonal table carries out orthogonal experiment and determine best embedding condition.The level of factor of orthogonal experiment is as shown in table 1, and the orthogonal experiment combination is as shown in table 2.
Table 1 orthogonal experiment level of factor table
Table 2 orthogonal experiment combination table
| Experiment numbers | A | B | C | D | E | F | |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 2 | 1 | 1 | 1 | 2 | 2 | 2 | 2 |
| 3 | 1 | 2 | 2 | 1 | 1 | 2 | 2 |
| 4 | 1 | 2 | 2 | 2 | 2 | 1 | 1 |
| 5 | 2 | 1 | 2 | 1 | 2 | 1 | 2 |
| 6 | 2 | 1 | 2 | 2 | 1 | 2 | 1 |
| 7 | 2 | 2 | 1 | 1 | 2 | 2 | 1 |
| 8 | 2 | 2 | 1 | 2 | 1 | 1 | 2 |
Microballoon to above eight kinds of fixing conditions carries out river bottom mud reparation contrast experiment, continues 45 days.
Determine best embedding condition with overlying water COD clearance as index.River course overlying water COD concentration has all experienced twice rising and decline process, and ultimate density is between 38mg/L-122mg/L, and the COD clearance is between 22.24%-71.87%.With the performance assessment criteria of COD clearance as definite microbial immobilized best embedding condition, determine that top condition is attapulgite concentration 0.5%, crosslinking time 36h, PVA concentration 8%, microbial inoculum concentration 15%, calcium carbonate concentration 0.4%, silica concentration 2.5%, 0.5%, seven factor of sodium alginate is to descending being arranged as of influence of immobilization embedded bead COD clearance: concentration of medium〉crosslinking time〉PVA concentration〉microbial inoculum concentration〉calcium carbonate concentration〉silica concentration〉sodium alginate concentration.
Table 3 with the COD clearance as the index orthogonal design table
In the table, K1 be the corresponding test index of 1 level and; K2 be the corresponding test index of 2 levels and; K1 be the corresponding test index of 1 level and mean number; K2 be the corresponding test index of 2 levels and mean number; Extreme difference R=k1-k2 in this experiment.Can the excellent level of factor of judgment and excellent combination according to K1, K2, k1, k2 size, can factor of judgment primary and secondary order according to extreme difference R size.
With overlying water NH
4 +-N clearance is determined best embedding condition as index.River course overlying water NH
4 +-N concentration rises earlier suddenly, continues to descend again, and is stabilized at last in the scope of a reduction.NH
4 +-N ultimate density between 0.46mg/L-1.44mg/L, NH
4 +-N clearance is between 77.00%-92.65%.With NH
4 +-N clearance is as the performance assessment criteria of determining microbial immobilized best embedding condition, determine that top condition is attapulgite concentration 0.5%, crosslinking time 36h, microbial inoculum concentration 15%, silica concentration 3.5%, sodium alginate concentration 1%, calcium carbonate concentration 0.4%, 8%, seven factor of PVA concentration is to descending being arranged as of influence of immobilization embedded bead COD clearance: concentration of medium〉crosslinking time〉microbial inoculum concentration〉silica concentration〉sodium alginate concentration〉calcium carbonate concentration〉PVA concentration.
Provide overlying water NO2--N and NO3--N concentration situation over time simultaneously.By Figure 4 and 5 as can be known, NO2--N and NO3--N concentration maintain the concentration of a reduction, and NO2--N concentration is between 0.11mg/L-0.91mg/L, and NO3--N concentration is between 0.11mg/L-2.69mg/L.
Utilize SPSS software, calculate eight covering device overlying water NH4+-N and NO2--N concentration Pearson correlation coefficient respectively, NH4+-N and NO3--N concentration Pearson correlation coefficient and NO2--N and NO3--N concentration Pearson correlation coefficient see table 3-1 for details.
Table 3-1
NH
4 +-N and NO
2 --N concentration, NH
4 +-N and NO
3 --N concentration and NO
2 --N and NO
3 -Dependency is little between the-N concentration, and three's Pearson correlation coefficient is minimum to be respectively 0.269,0.020 and 0.049.With traditional nitration denitrification process NH
4 +-N-NO
2 --N-NO
3 --N is also inconsistent.This is because the immobilized microorganism bead forms anaerobic zone, oxygen-starved area, aerobic zone from the inside to the outside successively, can form the anaerobic-aerobic environment simultaneously, denitrifying bacterium and aerobic bacteria be growth and breeding in adapt circumstance separately, carry out the synchronous nitration and denitrification reaction, be conducive to the removal of ammonia nitrogen, total nitrogen.
Table 4 is with NH
4 +-N clearance is as the index orthogonal design table
Determine best embedding condition with bed mud TOC clearance as index.River bottom mud TOC concentration has certain fluctuation in the early stage, and the later stage continues to reduce.The TOC ultimate density is between 914mg/L-1498mg/L, and the TOC clearance is between 38.34%-62.39%, as shown in Figure 6.With the performance assessment criteria of TOC clearance as definite microbial immobilized best embedding condition, determine that top condition is attapulgite concentration 0.5%, crosslinking time 36h, calcium carbonate concentration 0.4%, PVA concentration 8%, sodium alginate concentration 1%, silica concentration 2.5%, 15%, seven factor of microbial inoculum concentration is to descending being arranged as of influence of immobilization embedded bead TOC clearance: concentration of medium〉crosslinking time〉calcium carbonate concentration〉PVA concentration〉sodium alginate concentration〉silica concentration〉microbial inoculum concentration.
Table 5 with the TOC clearance as the index orthogonal design table
Respectively with overlying water COD, overlying water NH
4 +-N and bed mud TOC clearance be as index, and definite best embedding condition has only silica concentration and sodium alginate concentration different.With overlying water NH
4 +-N clearance is during as index, the influence of silica concentration and sodium alginate concentration is bigger, is major influence factors, and with overlying water COD and bed mud TOC clearance during as index, silica concentration and sodium alginate concentration influence are less, are the minor effect factor.Therefore determine attapulgite concentration 0.5%, crosslinking time 36h, microbial inoculum concentration 15%, silica concentration 3.5%, sodium alginate concentration 1%, calcium carbonate concentration 0.4%, PVA concentration 8% is best embedding condition.
Dissolved oxygen concentration and pH value are to influence the important factor that immobilized microorganism embedding bead is repaired riverbed bed mud ecology.
Research different dissolved oxygen concentration is to overlying water NH
4 +The influence of-N, TN and COD.Under the different dissolved oxygen concentration conditions, the active bead of immobilized microorganism that adds best embedding condition carries out river bottom mud reparation experiment, continues 45 days.Overlying water COD184mg/L, NH before adding
4 +-N6.67mg/L, TN7.5mg/L.When dissolved oxygen was between 2mg/L-3mg/L, ammonia nitrogen, total nitrogen and COD concentration were low after repairing, and between 0.85mg/L-0.91mg/L, 1.07mg/L-1.11mg/L and 38mg/L-44mg/L, clearance can reach about 87%, 85%, 79% respectively respectively.When the dissolved oxygen concentration increase, ammonia nitrogen, total nitrogen and COD clearance descend gradually.In sum, when dissolved oxygen was between 2mg/L-3mg/L, ammonia nitrogen, total nitrogen and COD clearance all reached the highest, referring to Fig. 7.
Study different pH values to overlying water NH
4 +The influence of-N, TN and COD.Under different pH condition, the active bead of immobilized microorganism that adds best embedding condition carries out river bottom mud reparation experiment, continues 45 days.Overlying water COD184mg/L, NH before adding
4 +-N6.67mg/L, TN7.5mg/L.The pH value is too high or too low, influences the activity of nitrobacteria and denitrifying bacterium.When between the pH value 7-8, ammonia nitrogen, total nitrogen and COD concentration are minimum, and between 1mg/L-1.07mg/L, 1.13mg/L-1.28mg/L and 35mg/L-50mg/L, clearance can reach about 85%, 85%, 81% respectively respectively.When the increase of pH concentration or reduction, ammonia nitrogen, total nitrogen and COD clearance descend gradually.In sum, when the pH value was between 7-8, ammonia nitrogen, total nitrogen and COD clearance all reached the highest, referring to Fig. 8.
Control dissolved oxygen concentration and pH value are between 2mg/L-3mg/L and 7-8, and the active bead of immobilized microorganism that adds best embedding condition carries out river bottom mud reparation experiment, continues 45 days.The bed mud total nitrogen is reduced to 1134mg/L from 2100mg/L, and clearance reaches 46%, sees Fig. 9 for details.Bed mud heterotrophic organism and the reversal of cure bacterium trend that all significantly decreases, heterotrophic organism is from 1.3*10
5Cell/g drops to 0.72*10
5Cell/g descends 45%; The reversal of cure bacterium is from 28*10
3Cell/g drops to 2.87*10
3Cell/g descends 90%, referring to Figure 10.Heterotrophic organism reduces gradually, shows that its nutritive substance of depending on for existence lacks, and organic substance constantly is degraded; Sulfide content and organic load, chemical aerobic amount positive correlation, the reversal of cure bacterium reduces, and shows alleviating of bed mud organic contamination, and the black smelly situation of bed mud obtains remarkable improvement.
Claims (5)
1. preparation method who is used for the immobilized microorganism embedding microballoon of riverbed bed mud ecosystem restoration, it is characterized in that: step is as follows:
The first step, preparation PVA gel:
Configuration concentration is 8～10% polyvinyl alcohol solution;
Add embedding medium in above-mentioned polyvinyl alcohol solution: concentration is that 0.5%～2% sodium alginate, the calcium carbonate of concentration 0.2%～0.5%, concentration are that 2.0～4.0% silicon-dioxide and concentration are 0.5～1.0% 300～500 order attapulgite powder or 300～500 order active carbon powders;
The profit that adds concentration and be after 10～15% activation is covered microbial inoculum, and the described sharp microbial inoculum that covers comprises two kinds of S-1 and N-1, and S-1 and N-1 volume ratio are 1:1;
Second step, linking agent preparation: take by weighing 7.5～8.0g boric acid, 3.5～4.0g Calcium Chloride Powder Anhydrous is dissolved in the 200mL water, is made into the saturated boric acid solution of calcium chloride of desired concn, and regulate pH to 7～8 with yellow soda ash;
The 3rd step, microballoon prepare: the PVA gel drops that mixes is added in the saturated boric acid solution of calcium chloride stirs, obtaining particle diameter is the active coccoid particle of immobilized microorganism of 3～5mm, is under 4～8 ℃ in temperature then, fixing crosslinked 24～36h, take out, use normal saline flushing.
2. the preparation method of the immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration according to claim 1, it is characterized in that: described attapulgite concentration is 0.5%, crosslinking time is 36h, PVA concentration is 8%, microbial inoculum concentration is 15%, calcium carbonate concentration is 0.4%, and silica concentration is 2.5%, and sodium alginate is 0.5%.
3. the preparation method of the immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration according to claim 1, it is characterized in that: described attapulgite concentration is 0.5%, crosslinking time is 36h, microbial inoculum concentration is 15%, silica concentration is 3.5%, sodium alginate concentration is 1%, and calcium carbonate concentration is that 0.4%, PVA concentration is 8%.
4. according to the preparation method of claim 1,2 or 3 described immobilized microorganism embedding microballoons for riverbed bed mud ecosystem restoration, it is characterized in that: the polyvinyl alcohol solution of described interpolation embedding medium stirs, and is cooled to 20～25 ℃ of 3～5h.
5. the preparation method of the immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration according to claim 4 is characterized in that: described S-1 is sharp, and to cover microbial inoculum be Bacillus subtilus, bacillus amyloliquefaciens, Bacillus licheniformis, fiber pseudomonas bacillus, Pseuomonas denitrifican, Rhodopseudomonas palustris, dinitrogen fiber bacterium or Pseudomonas stutzeri; The sharp illiteracy of N-1 microbial inoculum is Nitrosomonas, nitrosification spirillum, nitrosification leaf bacterium, nitrated thorn bacterium, Vickers bacterium nitrobacter or nitrated coccus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310264883.2A CN103275963B (en) | 2013-06-27 | 2013-06-27 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310264883.2A CN103275963B (en) | 2013-06-27 | 2013-06-27 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103275963A true CN103275963A (en) | 2013-09-04 |
| CN103275963B CN103275963B (en) | 2014-11-19 |
Family
ID=49058584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310264883.2A Active CN103275963B (en) | 2013-06-27 | 2013-06-27 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103275963B (en) |
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205412A (en) * | 2013-04-25 | 2013-07-17 | 西华大学 | Immobilized microbial preparation and method of treating meat product processing waste water by utilizing same |
| CN103523931A (en) * | 2013-10-25 | 2014-01-22 | 东南大学 | Method for repairing river-bed sediment ecological system by utilizing immobilized microorganism embedded microspheres |
| CN103849615A (en) * | 2013-12-31 | 2014-06-11 | 浙江省海洋开发研究院 | Immobilized algal-bacterial symbiotic system for processing petroleum pollutants and application thereof |
| CN104278019A (en) * | 2013-11-15 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
| CN104671626A (en) * | 2015-02-02 | 2015-06-03 | 江苏省海洋水产研究所 | Method for restoring eutrophic tidal flat sediment habitat |
| CN105523643A (en) * | 2015-12-25 | 2016-04-27 | 成都新柯力化工科技有限公司 | Sewage treatment composite gel material for microbe embedding, and preparation method thereof |
| CN105754984A (en) * | 2016-04-13 | 2016-07-13 | 四川农业大学 | Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof |
| CN106636060A (en) * | 2017-01-26 | 2017-05-10 | 同济大学 | Microbial immobilization carrier, microbial immobilization equipment and microbial immobilization method |
| TWI602606B (en) * | 2016-05-30 | 2017-10-21 | 國立中興大學 | Contaminated sediment gel separation method |
| CN108328737A (en) * | 2017-11-30 | 2018-07-27 | 河海大学 | A kind of Tiny ecosystem matrix, preparation method and its application based on artificial wet land reinforced processing breeding wastewater |
| CN108529759A (en) * | 2018-04-10 | 2018-09-14 | 北京华景山水生态科技有限公司 | A kind of water pollution sediment in-situ is biological reinforced to be handled and Benthic ecology restorative procedure |
| CN109609406A (en) * | 2018-12-27 | 2019-04-12 | 广州市金龙峰环保设备工程股份有限公司 | A kind of slow-release microbial bacterial agent and preparation method thereof administered for black and odorous water |
| CN110510849A (en) * | 2019-08-23 | 2019-11-29 | 清上(苏州)环境科技有限公司 | A kind of improvement of contaminated sediments original position and restorative procedure |
| CN110563292A (en) * | 2019-09-11 | 2019-12-13 | 海绵城市投资有限公司 | Riverway bottom mud repairing agent and preparation and application methods thereof |
| CN110577429A (en) * | 2018-06-08 | 2019-12-17 | 广东唐博生物科技有限公司 | double-layer embedded composite microbial fertilizer and preparation method thereof |
| CN111470614A (en) * | 2020-05-06 | 2020-07-31 | 北京工业大学 | Preparation and application of high-accumulation-rate short-cut nitrifying composite bacteria embedded bioactive filler |
| CN111892156A (en) * | 2020-07-03 | 2020-11-06 | 广州博嵩生物环保科技有限公司 | Porous degradable material and preparation method and application thereof |
| CN112723530A (en) * | 2020-12-11 | 2021-04-30 | 温州大学 | Preparation method of immobilized composite filler |
| CN114108319A (en) * | 2021-12-03 | 2022-03-01 | 山西大学 | Preparation method and application of Shewanella embedded gel fiber |
| CN114275877A (en) * | 2021-12-30 | 2022-04-05 | 青岛威羽山环保科技有限公司 | Novel soft filler and preparation process thereof |
| CN114774402A (en) * | 2022-04-19 | 2022-07-22 | 中交上海航道勘察设计研究院有限公司 | Method for cultivating and immobilizing microorganisms |
| CN114853153A (en) * | 2022-04-07 | 2022-08-05 | 航天凯天环保科技股份有限公司 | Microorganism-combined PRB (reactive Barrier concrete) remediation system and application thereof in-situ remediation of antibiotic-polluted groundwater |
| CN114890841A (en) * | 2022-03-24 | 2022-08-12 | 武汉绿农瑞益生物科技有限公司 | Long-acting microbial compound fertilizer and preparation method thereof |
| CN114933509A (en) * | 2022-05-09 | 2022-08-23 | 武汉绿农瑞益生物科技有限公司 | Slow-release compound fertilizer and preparation method thereof |
| CN115708491A (en) * | 2022-11-16 | 2023-02-24 | 江苏海洋大学 | Culture substrate of buried shellfish and application thereof in buried shellfish culture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001299340A (en) * | 2000-04-26 | 2001-10-30 | Kansai Paint Co Ltd | Method for regulating specific gravity of enzyme or microbial immobilized carrier |
| CN102079571A (en) * | 2010-11-22 | 2011-06-01 | 兰州交通大学 | Nano attapulgite composite polyvinyl acetal foam microorganism immobilized carrier |
| CN103114085A (en) * | 2013-01-18 | 2013-05-22 | 西安建筑科技大学 | Preparation of SRB (sulfate reducing bacterium) biological inhibitor and method for inhibiting SRB in crude oil |
-
2013
- 2013-06-27 CN CN201310264883.2A patent/CN103275963B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001299340A (en) * | 2000-04-26 | 2001-10-30 | Kansai Paint Co Ltd | Method for regulating specific gravity of enzyme or microbial immobilized carrier |
| CN102079571A (en) * | 2010-11-22 | 2011-06-01 | 兰州交通大学 | Nano attapulgite composite polyvinyl acetal foam microorganism immobilized carrier |
| CN103114085A (en) * | 2013-01-18 | 2013-05-22 | 西安建筑科技大学 | Preparation of SRB (sulfate reducing bacterium) biological inhibitor and method for inhibiting SRB in crude oil |
Non-Patent Citations (1)
| Title |
|---|
| 杨慧: "添加吸附剂对包埋固定化微生物凝胶小球性能的影响研究", 《中国优秀硕士学位论文数据库》 * |
Cited By (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205412B (en) * | 2013-04-25 | 2016-04-13 | 西华大学 | The method of immobilized microorganism preparation and employing said preparation process meat product processing waste water |
| CN103205412A (en) * | 2013-04-25 | 2013-07-17 | 西华大学 | Immobilized microbial preparation and method of treating meat product processing waste water by utilizing same |
| CN103523931A (en) * | 2013-10-25 | 2014-01-22 | 东南大学 | Method for repairing river-bed sediment ecological system by utilizing immobilized microorganism embedded microspheres |
| CN104278019A (en) * | 2013-11-15 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
| CN103849615A (en) * | 2013-12-31 | 2014-06-11 | 浙江省海洋开发研究院 | Immobilized algal-bacterial symbiotic system for processing petroleum pollutants and application thereof |
| CN104671626B (en) * | 2015-02-02 | 2017-03-29 | 江苏省海洋水产研究所 | A kind of method of eutrophication Tidal Flat Sediments habitat rehabilitation |
| CN104671626A (en) * | 2015-02-02 | 2015-06-03 | 江苏省海洋水产研究所 | Method for restoring eutrophic tidal flat sediment habitat |
| CN105523643A (en) * | 2015-12-25 | 2016-04-27 | 成都新柯力化工科技有限公司 | Sewage treatment composite gel material for microbe embedding, and preparation method thereof |
| CN105523643B (en) * | 2015-12-25 | 2018-02-06 | 林立荣 | A kind of sewage disposal composite gel material of imbedded microbe and preparation method thereof |
| CN105754984A (en) * | 2016-04-13 | 2016-07-13 | 四川农业大学 | Sodium alginate compound immobilized microbial agent as well as preparation method and application thereof |
| CN105754984B (en) * | 2016-04-13 | 2019-03-12 | 四川农业大学 | Compound immobilized microbial inoculum of sodium alginate and preparation method thereof and purposes |
| TWI602606B (en) * | 2016-05-30 | 2017-10-21 | 國立中興大學 | Contaminated sediment gel separation method |
| CN106636060A (en) * | 2017-01-26 | 2017-05-10 | 同济大学 | Microbial immobilization carrier, microbial immobilization equipment and microbial immobilization method |
| CN108328737A (en) * | 2017-11-30 | 2018-07-27 | 河海大学 | A kind of Tiny ecosystem matrix, preparation method and its application based on artificial wet land reinforced processing breeding wastewater |
| CN108529759A (en) * | 2018-04-10 | 2018-09-14 | 北京华景山水生态科技有限公司 | A kind of water pollution sediment in-situ is biological reinforced to be handled and Benthic ecology restorative procedure |
| CN110577429A (en) * | 2018-06-08 | 2019-12-17 | 广东唐博生物科技有限公司 | double-layer embedded composite microbial fertilizer and preparation method thereof |
| CN109609406A (en) * | 2018-12-27 | 2019-04-12 | 广州市金龙峰环保设备工程股份有限公司 | A kind of slow-release microbial bacterial agent and preparation method thereof administered for black and odorous water |
| CN112441713B (en) * | 2019-08-23 | 2022-09-20 | 清上(苏州)环境科技有限公司 | In-situ remediation composite material and in-situ treatment and remediation method for polluted bottom mud |
| CN112441713A (en) * | 2019-08-23 | 2021-03-05 | 清上(苏州)环境科技有限公司 | In-situ remediation composite material and in-situ treatment and remediation method for polluted bottom mud |
| CN110510849A (en) * | 2019-08-23 | 2019-11-29 | 清上(苏州)环境科技有限公司 | A kind of improvement of contaminated sediments original position and restorative procedure |
| CN110563292B (en) * | 2019-09-11 | 2022-03-01 | 海绵城市投资有限公司 | Riverway bottom mud repairing agent and preparation and application methods thereof |
| CN110563292A (en) * | 2019-09-11 | 2019-12-13 | 海绵城市投资有限公司 | Riverway bottom mud repairing agent and preparation and application methods thereof |
| CN111470614A (en) * | 2020-05-06 | 2020-07-31 | 北京工业大学 | Preparation and application of high-accumulation-rate short-cut nitrifying composite bacteria embedded bioactive filler |
| CN111892156A (en) * | 2020-07-03 | 2020-11-06 | 广州博嵩生物环保科技有限公司 | Porous degradable material and preparation method and application thereof |
| CN111892156B (en) * | 2020-07-03 | 2022-05-03 | 广州博嵩生物环保科技有限公司 | Porous degradable material and preparation method and application thereof |
| CN112723530A (en) * | 2020-12-11 | 2021-04-30 | 温州大学 | Preparation method of immobilized composite filler |
| CN114108319A (en) * | 2021-12-03 | 2022-03-01 | 山西大学 | Preparation method and application of Shewanella embedded gel fiber |
| CN114108319B (en) * | 2021-12-03 | 2023-09-22 | 山西大学 | Preparation method and application of gel fiber embedded with Shewanella |
| CN114275877A (en) * | 2021-12-30 | 2022-04-05 | 青岛威羽山环保科技有限公司 | Novel soft filler and preparation process thereof |
| CN114890841A (en) * | 2022-03-24 | 2022-08-12 | 武汉绿农瑞益生物科技有限公司 | Long-acting microbial compound fertilizer and preparation method thereof |
| CN114853153A (en) * | 2022-04-07 | 2022-08-05 | 航天凯天环保科技股份有限公司 | Microorganism-combined PRB (reactive Barrier concrete) remediation system and application thereof in-situ remediation of antibiotic-polluted groundwater |
| CN114853153B (en) * | 2022-04-07 | 2023-12-15 | 航天凯天环保科技股份有限公司 | Microorganism-combined PRB repair system and application thereof in-situ repair of antibiotic-polluted groundwater |
| CN114774402A (en) * | 2022-04-19 | 2022-07-22 | 中交上海航道勘察设计研究院有限公司 | Method for cultivating and immobilizing microorganisms |
| CN114933509A (en) * | 2022-05-09 | 2022-08-23 | 武汉绿农瑞益生物科技有限公司 | Slow-release compound fertilizer and preparation method thereof |
| CN115708491A (en) * | 2022-11-16 | 2023-02-24 | 江苏海洋大学 | Culture substrate of buried shellfish and application thereof in buried shellfish culture |
| CN115708491B (en) * | 2022-11-16 | 2024-02-20 | 江苏海洋大学 | Culture substrate of buried shellfish and application of culture substrate in buried shellfish culture |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103275963B (en) | 2014-11-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103275963B (en) | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem | |
| Zhi et al. | Enhanced long-term nitrogen removal and its quantitative molecular mechanism in tidal flow constructed wetlands | |
| CN103013872B (en) | Heterotrophic nitrifying and aerobic denitrifying bacterium and culture and application thereof | |
| Wang et al. | Advances in microbially mediated manganese redox cycling coupled with nitrogen removal in wastewater treatment: A critical review and bibliometric analysis | |
| Hatamoto et al. | Characterization of downflow hanging sponge reactors with regard to structure, process function, and microbial community compositions | |
| Han et al. | Enhanced nitrogen removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine wastewater | |
| CN109879415B (en) | Slow-release electron donor and method for deep denitrification of sewage by using same | |
| CN101565258B (en) | Compound technology of biomembrane and granular sludge for treating sulfur-bearing organic wastewater | |
| CN101402485B (en) | Cohesive action nutrient source SRB sewage sludge immobilization particle, production and uses in treating heavy metal wastewater thereof | |
| CN101831392B (en) | Autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent as well as culture method and application thereof | |
| Bae et al. | High-rate partial nitritation using porous poly (vinyl alcohol) sponge | |
| CN101007678A (en) | Temperature controlled USAB anaerobic ammoxidation strain denitrogenation method and its apparatus | |
| CN105858880A (en) | Method for treating municipal sewage and nitrate wastewater by immobilized anaerobic ammoxidation coupled short-range denitrification | |
| Wang et al. | Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis | |
| Tan et al. | Effects of tourmaline on nitrogen removal performance and biofilm structures in the sequencing batch biofilm reactor | |
| WO2022229224A1 (en) | Immobilized micbrobes for water treatment | |
| CN107162187A (en) | A kind of preparation method of immobilization Anammox bacterium mud and its method for sewage disposal | |
| CN117069274A (en) | Electric field enhanced vertical flow constructed wetland device | |
| Chi et al. | Nitrogen removal performance and bacterial flora analysis of a partial nitrification-anaerobic ammonium oxidation-denitrification system treating rare earth element tailings wastewater | |
| CN1962483A (en) | Biological sponge iron preparation and its uses in water disposal | |
| CN103074285B (en) | High-salt heterotrophic nitrification-aerobic denitrification dephosphorization brachybacterium and application of brachybacterium in wastewater treatment | |
| CN110699347A (en) | Immobilized microbial inoculum and preparation method and application thereof | |
| Muñoz-Palazón et al. | Simultaneous Nitrification and Denitrification Processes in Granular Sludge Technology | |
| CN116371383A (en) | Reinforced dephosphorization composite filler, and preparation method and application thereof | |
| Xin et al. | Integrated effects of operational temperature, HRT, and influent ammonium concentration on a CANON coupling with denitrification process treating for digested piggery wastewater: performance and microbial community |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |