CN103275963B - Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem - Google Patents
Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem Download PDFInfo
- Publication number
- CN103275963B CN103275963B CN201310264883.2A CN201310264883A CN103275963B CN 103275963 B CN103275963 B CN 103275963B CN 201310264883 A CN201310264883 A CN 201310264883A CN 103275963 B CN103275963 B CN 103275963B
- Authority
- CN
- China
- Prior art keywords
- concentration
- preparation
- immobilized microorganism
- pva
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/40—Protecting water resources
- Y02A20/402—River restoration
Abstract
The invention discloses a preparation method of an immobilized microorganism embedding microsphere for restoring a riverbed bottom mud ecosystem. The preparation method comprises the steps of PVA (polyvinyl alcohol) gel preparation, cross-linking agent preparation and microsphere preparation, wherein the PVA gel preparation comprises the steps of preparing a PVA solution with a concentration of 8-10%, adding embedding agents to the PVA solution, namely sodium alginate with a concentration of 0.5-2%, calcium carbonate with a concentration of 0.2-0.5%, silicon dioxide with a concentration of 2.0-4.0%, and 300-500 mesh attapulgite powder or 300-500 mesh activated carbon powder with a concentration of 0.5-1.0%, and adding activated limon microorganism bacterium liquid with a concentration of 10-15%; and the microsphere preparation comprises the steps of dropwise adding PVA gel to a calcium chloride saturated boric acid solution, stirring, obtaining immobilized microorganism activated spherular particles with the particle size of 3-5mm, conducting immobilized crosslinking for 24-36h at 4-8 DEG C, taking out, and washing with normal saline. Compared with the prior art, the microsphere can remove total organic carbon and total nitrogen in riverbed bottom mud effectively, and can improve COD (chemical oxygen demand), ammonia nitrogen and total nitrogen pollution conditions of an overlying water body of the bottom mud, and the preparation method is an ecological riverway management scheme which is efficient, low-consumption and simple to operate, and has a development prospect.
Description
Technical field:
The present invention relates to a kind of immobilized microorganism embedding treatment method for riverbed bed mud ecosystem restoration, specifically the embedding method for preparing microsphere of microorganism embedding treatment method.
Background technology:
Stream pollution is one of ubiquitous outstanding environmental problem in current global range, as the important component part of river ecosystem, bed mud is not only the key link of all kinds of material cycle in river, and is the main gathering storehouse of these materials---and bed mud is pollution substance in water body " storehouse " and " source ".Therefore, research and improvement that bed mud in river pollutes, be the important content of stream pollution comprehensive improvement, is one of important channel fundamentally solving stream pollution problem.
River bottom mud reparation mainly contains physics reparation, chemistry is repaired and biological restoration three major types recovery technique.Physics reparation comprises dredging and covering etc., environmental dredging cost consumption power, and easily original ecotope is caused damage, water correction effect is very unobvious yet; Soverlay technique cannot fundamentally be removed pollutent, uses in a large number covering material can reduce lake storage capacity, and searching covering material material source also becomes problem.Chemistry reparation need to add chemical agent in water, and investment is large, destroys river ecosystem, and easily produces secondary pollution.
Immobilized microorganism method is advanced in the world water body treating method, adopts certain technique means (absorption method, entrapping method, crosslinking and blocking cut-off process etc.) to make microorganism set growth, and microorganism is not suspended in water and still keeps biological activity, and can recycle.Entrapping method is that microorganism is fixed in specific polymer carrier grid structure, utilizes carrier self structure characteristic, makes the small-molecule substance carrier of freely coming in and going out, and with inner microbial interaction, reaches the effect of removing pollutent.Adopt immobilized microorganism technique, can effectively degrade organic wastewater with difficult degradation thereby, heavy metal wastewater thereby and ammonia nitrogen waste water etc.From environment, screening has the microbial population of the specific hardly degraded organic substances such as degraded phenol, lipid, and applying immobilized microbial technique is implemented immobilization to it, and degradation efficiency is obviously better than traditional biologic treating technique; A large amount of algae and microbial cells can effectively absorb with enrichment sewage in heavy metal, compared with free suspended alga and thalline, immobilization can reduce the toxic action of Heavy Metals on Algae and thalline, shows great potentiality processing in heavy metal wastewater thereby; Immobilized microorganism technique can form anaerobic zone and aerobic zone inside and outside particle simultaneously, and nitration reaction and anti-nitration reaction can complete in same reactor, can strengthened denitrification effect, become gradually environmentalist's study hotspot.
Oneself has been subjected to concern widely the particular advantages of immobilized microorganism technique aspect water treatment, but its research at riverbed bed mud ecosystem restoration and application are almost blank.After point-source pollution (sewage discharge belongs to point-source pollution) is effectively controlled, bed mud just becomes the important endogenous of River Water Pollution, a large amount of hazardous contaminants of accumulating in bed mud, again discharging by certain exchange interaction, is the main secondary pollution source of impact and restriction overlying water matter.How effectively to control riverbed sediment pollution, improve water quality of river, become most important environmental problem in China's water surrounding.
Summary of the invention:
The present invention carries and will solve to such an extent that technical problem is for above-mentioned the deficiencies in the prior art, and the microorganism embedding method for preparing microsphere that provides the bed mud embedding of a kind of riverbed to administer, can effectively remove total organic carbon and the total nitrogen in riverbed bed mud by microballoon of the present invention, improving bed mud overlying water COD, ammonia nitrogen and total nitrogen pollution situation, is a kind of efficient low-consume, simple to operate, promising river channel ecology resolution.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
For a preparation method for the immobilized microorganism embedding microballoon of riverbed bed mud ecosystem restoration, it is characterized in that: step is as follows:
1, preparation PVA gel:
Configuration concentration is 8～10% polyvinyl alcohol solution;
In above-mentioned polyvinyl alcohol solution, add embedding medium: 300～500 order attapulgite powder that the silicon-dioxide that the sodium alginate that concentration is 0.5%～2%, the calcium carbonate of concentration 0.2%～0.5%, concentration are 2.0～4.0% and concentration are 0.5～1.0% or 300～500 order active carbon powders;
Adding concentration is that profit after 10～15% activation is covered microbial inoculum, and described profit is covered microbial inoculum and comprised two kinds of S-1 and N-1, and S-1 and N-1 volume ratio are 1:1;
2, linking agent preparation: take 7.5～8.0g boric acid, 3.5～4.0g Calcium Chloride Powder Anhydrous is dissolved in 200mL water, is made into the saturated boric acid solution of calcium chloride of desired concn, and regulate pH to 7～8 with sodium carbonate;
3, microballoon preparation: the PVA gel drops mixing is added in the saturated boric acid solution of calcium chloride and is stirred, obtaining particle diameter is the active coccoid particle of immobilized microorganism of 3～5mm, is then at 4～8 DEG C in temperature, fixing crosslinked 24～36h, take out, use normal saline flushing.
Described attapulgite concentration is 0.5%, and crosslinking time is 36h, and PVA concentration is 8%, and microbial inoculum concentration is 15%, and calcium carbonate concentration is 0.4%, and silica concentration is 2.5%, and sodium alginate is 0.5%.
Described attapulgite concentration is 0.5%, and crosslinking time is 36h, and microbial inoculum concentration is 15%, and silica concentration is 3.5%, and sodium alginate concentration is 1%, and calcium carbonate concentration is that 0.4%, PVA concentration is 8%.
The polyvinyl alcohol solution of described interpolation embedding medium stirs, and is cooled to 20～25 DEG C of 3～5h.
It is Bacillus subtilus, bacillus amyloliquefaciens, Bacillus licheniformis, fiber pseudomonas bacillus, Pseuomonas denitrifican, Rhodopseudomonas palustris, dinitrogen fiber bacterium or Pseudomonas stutzeri that described S-1 profit is covered microbial inoculum; It is Nitrosomonas, nitrosification spirillum, nitrosification leaf bacterium, nitrated thorn bacterium, Vickers bacterium nitrobacter or nitrated coccus that N-1 profit is covered microbial inoculum.
In the process of the active bead of preparation immobilized microorganism, the factor that affects immobilized spherule degradation of contaminant mainly contains PVA gel strength, embedding medium concentration, microbial inoculum concentration and crosslinking time.
PVA concentration is too low, and pellet hardness is inadequate, and difficult forming is easily broken; PVA excessive concentration, gel densification, strengthens matrix resistance to mass transfer, and treatment effect is bad.In sum, select PVA concentration range between 8% and 10%.
In embedding carrier fixation of microbe process, add in proportion a small amount of sodium alginate, silicon-dioxide and calcium carbonate, permeability and the physical strength of PVA particle are all improved to some extent.Add active carbon powder or attapulgite powder, both strengthened the mass transfer ability of biological bead, improved again its physical strength.On previous experiments basis, choose sodium alginate concentration between 0.5% and 1%, calcium carbonate concentration is between 0.2% and 0.4%, and silica concentration is between 2.5% and 3.5%, between 300 order active carbon powders or 300 order attapulgite powder concentration 0.5% and 1%.
Microbial inoculum concentration has determined the content of microorganism in immobilized microorganism bead, and the removal of pollution substance is had a direct impact.Microbial inoculum concentration is too low, and contaminants removal is not thorough; Microbial inoculum excessive concentration, microorganism is easily separated out.On previous experiments basis, choose microbial inoculum concentration between 10% and 15%.
Crosslinking time refers to the molding time of immobilized microorganism bead in saturated boric acid solution.Crosslinking time is too short, bead insufficient strength; Crosslinking time is long, because boric acid is to the toxic effect of microorganism, can reduce cytoactive.On previous experiments basis, choose crosslinking time between 24h and 36h.
Compared with prior art, microballoon of the present invention can effectively be removed total organic carbon and the total nitrogen in riverbed bed mud, improves bed mud overlying water COD, ammonia nitrogen and total nitrogen pollution situation, is a kind of efficient low-consume, simple to operate, promising river channel ecology resolution.
Brief description of the drawings
The preparation flow schematic diagram of the active small spherical particles of Fig. 1 immobilized microorganism;
Fig. 2 overlying water COD concentration changes with time situation schematic diagram;
Fig. 3 overlying water NH
4 +-N concentration changes with time situation schematic diagram;
Fig. 4 overlying water NO
2 --N concentration changes with time situation schematic diagram;
Fig. 5 overlying water NO
3 --N concentration changes with time situation schematic diagram;
Fig. 6 bed mud TOC concentration changes with time situation schematic diagram;
The affect schematic diagram of Fig. 7 different dissolved oxygen concentration on ammonia nitrogen, total nitrogen and COD treatment effect;
The affect schematic diagram of the different pH values of Fig. 8 on ammonia nitrogen, total nitrogen and COD treatment effect;
Fig. 9 bed mud total nitrogen concentration temporal evolution situation schematic diagram;
Figure 10 bed mud heterotrophic organism and reversal of cure bacterial number temporal evolution situation schematic diagram.
Embodiment
Below in conjunction with accompanying drawing, the present invention is elaborated:
The preparation method of a kind of immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration of the present invention, its process step is as shown in Figure 1, specific as follows:
1, preparation PVA gel:
Configuration concentration is 8～10% polyvinyl alcohol solution: in above-mentioned polyvinyl alcohol solution, add embedding medium: 300～500 order attapulgite powder that the silicon-dioxide that the sodium alginate that concentration is 0.5%～2%, the calcium carbonate of concentration 0.2%～0.5%, concentration are 2.0～4.0% and concentration are 0.5～1.0% or 300～500 order active carbon powders; Adding concentration is that profit after 10～15% activation is covered microbial inoculum, and described profit is covered microbial inoculum and comprised two kinds of S-1 and N-1, and S-1 and N-1 volume ratio are 1:1;
Specifically can adopt configuration with the following method: take 7.5～10.0g polyvinyl alcohol by concentration requirement and add in 100ml water, polyvinyl alcohol is 1788 types preferably.After infiltrating 24h, use thermostat water bath to dissolve completely at 85～90 DEG C, then add a certain amount of embedding medium (sodium alginate 0.5～2.0g, silicon-dioxide 2.0～4.0g, calcium carbonate 0.～0.5g, 300 order attapulgite powder or 300 order active carbon powder 0.5～1.0g) stir, be cooled to 20～25 DEG C of 3h-5h, (profit is covered microbial inoculum and is comprised two kinds of S-1 and N-1 to add profit after 10～15% activation to cover microbial inoculum, S-1: Bacillus subtilus, bacillus amyloliquefaciens, Bacillus licheniformis, fiber pseudomonas bacillus, Pseuomonas denitrifican, Rhodopseudomonas palustris, dinitrogen fiber bacterium, Pseudomonas stutzeri etc., N-1: Nitrosomonas, nitrosification spirillum, nitrosification leaf bacterium, nitrated thorn bacterium, Vickers bacterium nitrobacter, nitrated coccus etc.S-1 and N-1 volume ratio are 1:1.）
2, linking agent preparation: take 7.5～8.0g boric acid, 3.5～4.0g Calcium Chloride Powder Anhydrous is dissolved in 200mL water, is made into the saturated boric acid solution of calcium chloride of desired concn, and regulate pH to 7～8 with sodium carbonate;
3, microballoon preparation: the PVA gel drops mixing is added in the saturated boric acid solution of calcium chloride, specifically can adopt peristaltic pump to add, and use magnetic stirring apparatus constantly to stir, obtaining particle diameter is the active coccoid particle of immobilized microorganism of 3～5mm, then be at 4～8 DEG C in temperature, fixing crosslinked 24～36h, takes out, and uses normal saline flushing.
Taking calcium carbonate concentration (A), silica concentration (B), active carbon powder or attapulgite powder concentration (C), sodium alginate concentration (D), crosslinking time (E), microbial inoculum concentration (F) and PVA concentration (G) as influence factor, each factor is chosen two levels, adopts L
8(2
7) orthogonal table carries out orthogonal experiment and determine best embedding conditions.The level of factor of orthogonal experiment is as shown in table 1, and orthogonal experiment combination is as shown in table 2.
Table 1 orthogonal experiment level of factor table
Table 2 orthogonal experiment combination table
| Experiment numbers | A | B | C | D | E | F | G |
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 2 | 1 | 1 | 1 | 2 | 2 | 2 | 2 |
| 3 | 1 | 2 | 2 | 1 | 1 | 2 | 2 |
| 4 | 1 | 2 | 2 | 2 | 2 | 1 | 1 |
| 5 | 2 | 1 | 2 | 1 | 2 | 1 | 2 |
| 6 | 2 | 1 | 2 | 2 | 1 | 2 | 1 |
| 7 | 2 | 2 | 1 | 1 | 2 | 2 | 1 |
| 8 | 2 | 2 | 1 | 2 | 1 | 1 | 2 |
The microballoon of above eight kinds of fixing conditions is carried out to river bottom mud reparation contrast experiment, continue 45 days.
Determine best embedding conditions using overlying water COD clearance as index.River course overlying water COD concentration has all experienced twice rising and decline process, and ultimate density is between 38mg/L-122mg/L, and COD clearance is between 22.24%-71.87%.Using COD clearance as the performance assessment criteria of determining microbial immobilized best embedding conditions, determine that top condition is attapulgite concentration 0.5%, crosslinking time 36h, PVA concentration 8%, microbial inoculum concentration 15%, calcium carbonate concentration 0.4%, silica concentration 2.5%, sodium alginate 0.5%, seven factors descending being arranged as of impact on immobilization embedded bead COD clearance: concentration of medium > crosslinking time >PVA concentration > microbial inoculum concentration > calcium carbonate concentration > silica concentration > sodium alginate concentration.
Table 3 is using COD clearance as index orthogonal design table
In table, K1 be the corresponding test index of 1 level and; K2 be the corresponding test index of 2 level and; K1 be the corresponding test index of 1 level and mean number; K2 be the corresponding test index of 2 level and mean number; Extreme difference R=k1-k2 in this experiment.Can the excellent level of factor of judgment and excellent combination according to K1, K2, k1, k2 size, can factor of judgment primary and secondary order according to extreme difference R size.
With overlying water NH
4 +-N clearance is determined best embedding conditions as index.River course overlying water NH
4 +-N concentration first rises suddenly, then continuous decrease, is finally stabilized in the scope of a reduction.NH
4 +-N ultimate density between 0.46mg/L-1.44mg/L, NH
4 +-N clearance is between 77.00%-92.65%.With NH
4 +-N clearance is as the performance assessment criteria of determining microbial immobilized best embedding conditions, determine that top condition is attapulgite concentration 0.5%, crosslinking time 36h, microbial inoculum concentration 15%, silica concentration 3.5%, sodium alginate concentration 1%, calcium carbonate concentration 0.4%, PVA concentration 8%, seven factors descending being arranged as of impact on immobilization embedded bead COD clearance: concentration of medium > crosslinking time > microbial inoculum concentration > silica concentration > sodium alginate concentration > calcium carbonate concentration >PVA concentration.
Provide overlying water NO2--N and NO3--N concentration situation over time simultaneously.From Figure 4 and 5, NO2--N and NO3--N concentration maintain the concentration of a reduction, and NO2--N concentration is between 0.11mg/L-0.91mg/L, and NO3--N concentration is between 0.11mg/L-2.69mg/L.
Utilize SPSS software, calculate respectively eight covering device overlying water NH4+-N and NO2--N concentration Pearson correlation coefficient, NH4+-N and NO3--N concentration Pearson correlation coefficient and NO2--N and NO3--N concentration Pearson correlation coefficient, refer to table 3-1.
Table 3-1
NH
4 +-N and NO
2 --N concentration, NH
4 +-N and NO
3 --N concentration and NO
2 --N and NO
3 -between-N concentration, dependency is little, and three's Pearson correlation coefficient is minimum is respectively 0.269,0.020 and 0.049.With traditional nitration denitrification process NH
4 +-N-NO
2 --N-NO
3 --N is also inconsistent.This is because immobilized microorganism bead forms anaerobic zone, oxygen-starved area, aerobic zone from the inside to the outside successively, can form anaerobic-aerobic environment simultaneously, denitrifying bacterium and aerobic bacteria be growth and breeding in adapt circumstance separately, carry out synchronous nitration and denitrification reaction, be conducive to the removal of ammonia nitrogen, total nitrogen.
Table 4 is with NH
4 +-N clearance is as index orthogonal design table
Determine best embedding conditions using bed mud TOC clearance as index.River bottom mud TOC concentration has certain fluctuation in the early stage, and the later stage continues to reduce.TOC ultimate density is between 914mg/L-1498mg/L, and TOC clearance is between 38.34%-62.39%, as shown in Figure 6.Using TOC clearance as the performance assessment criteria of determining microbial immobilized best embedding conditions, determine that top condition is attapulgite concentration 0.5%, crosslinking time 36h, calcium carbonate concentration 0.4%, PVA concentration 8%, sodium alginate concentration 1%, silica concentration 2.5%, microbial inoculum concentration 15%, seven factors descending being arranged as of impact on immobilization embedded bead TOC clearance: concentration of medium > crosslinking time > calcium carbonate concentration >PVA concentration > sodium alginate concentration > silica concentration > microbial inoculum concentration.
Table 5 is using TOC clearance as index orthogonal design table
Respectively with overlying water COD, overlying water NH
4 +-N and bed mud TOC clearance be as index, definite best embedding conditions only have silica concentration and sodium alginate concentration different.With overlying water NH
4 +-N clearance is during as index, the impact of silica concentration and sodium alginate concentration is larger, is major influence factors, and using overlying water COD and bed mud TOC clearance during as index, silica concentration and sodium alginate concentration impact are less, are minor effect factor.Therefore determine attapulgite concentration 0.5%, crosslinking time 36h, microbial inoculum concentration 15%, silica concentration 3.5%, sodium alginate concentration 1%, calcium carbonate concentration 0.4%, PVA concentration 8% is best embedding conditions.
Dissolved oxygen concentration and pH value are the important factors that affects immobilized microorganism embedding bead reparation riverbed bed mud ecology.
Research different dissolved oxygen concentration is to overlying water NH
4 +the impact of-N, TN and COD.Under different dissolved oxygen concentration conditions, the active bead of immobilized microorganism that adds best embedding conditions carries out river bottom mud reparative experiment, continues 45 days.Add front overlying water COD184mg/L, NH
4 +-N6.67mg/L, TN7.5mg/L.In the time that dissolved oxygen is between 2mg/L-3mg/L, after repairing, ammonia nitrogen, total nitrogen and COD concentration are low, and respectively between 0.85mg/L-0.91mg/L, 1.07mg/L-1.11mg/L and 38mg/L-44mg/L, clearance can reach respectively 87%, 85%, 79% left and right.When dissolved oxygen concentration increases, ammonia nitrogen, total nitrogen and COD clearance decline gradually.In sum, in the time that dissolved oxygen is between 2mg/L-3mg/L, ammonia nitrogen, total nitrogen and COD clearance all reach the highest, referring to Fig. 7.
Study different pH values to overlying water NH
4 +the impact of-N, TN and COD.Under different pH condition, the active bead of immobilized microorganism that adds best embedding conditions carries out river bottom mud reparative experiment, continues 45 days.Add front overlying water COD184mg/L, NH
4 +-N6.67mg/L, TN7.5mg/L.PH value is too high or too low, affects the activity of nitrobacteria and denitrifying bacterium.When between pH value 7-8, ammonia nitrogen, total nitrogen and COD concentration are minimum, and respectively between 1mg/L-1.07mg/L, 1.13mg/L-1.28mg/L and 35mg/L-50mg/L, clearance can reach respectively 85%, 85%, 81% left and right.When pH concentration increases or reduces, ammonia nitrogen, total nitrogen and COD clearance decline gradually.In sum, in the time that pH value is between 7-8, ammonia nitrogen, total nitrogen and COD clearance all reach the highest, referring to Fig. 8.
Control dissolved oxygen concentration and pH value between 2mg/L-3mg/L and 7-8, the active bead of immobilized microorganism that adds best embedding conditions carries out river bottom mud reparative experiment, continues 45 days.Bed mud total nitrogen is reduced to 1134mg/L from 2100mg/L, and clearance reaches 46%, refers to Fig. 9.Bed mud heterotrophic organism and the reversal of cure bacterium trend that all significantly decreases, heterotrophic organism is from 1.3*10
5cell/g drops to 0.72*10
5cell/g, declines 45%; Reversal of cure bacterium is from 28*10
3cell/g drops to 2.87*10
3cell/g, declines 90%, referring to Figure 10.Heterotrophic organism reduces gradually, shows that its nutritive substance of depending on for existence lacks, and organic substance is constantly degraded; Sulfide content and organic load, chemical oxygen demand positive correlation, reversal of cure bacterium reduces, and shows alleviating of bed mud organic contamination, and the black smelly situation of bed mud obtains remarkable improvement.
Claims (4)
1. for a preparation method for the immobilized microorganism embedding microballoon of riverbed bed mud ecosystem restoration, it is characterized in that: step is as follows:
The first step, prepare polyvinyl alcohol gel:
Configuration concentration is 8～10% polyvinyl alcohol solution;
In above-mentioned polyvinyl alcohol solution, add embedding medium: 300～500 order attapulgite powder that the silicon-dioxide that the sodium alginate that concentration is 0.5%～2%, the calcium carbonate of concentration 0.2%～0.5%, concentration are 2.0～4.0% and concentration are 0.5～1.0% or 300～500 order active carbon powders;
Adding concentration is the profit illiteracy microbial inoculum after 10～15% activation, described profit is covered microbial inoculum and is comprised two kinds of S-1 and N-1, S-1 and N-1 volume ratio are 1:1, and it is Bacillus subtilus, bacillus amyloliquefaciens, Bacillus licheniformis, fiber pseudomonas bacillus, Pseuomonas denitrifican, Rhodopseudomonas palustris, dinitrogen fiber bacterium or Pseudomonas stutzeri that described S-1 profit is covered microbial inoculum; It is Nitrosomonas, nitrosification spirillum, nitrosification leaf bacterium, nitrated thorn bacterium, Vickers bacterium nitrobacter or nitrated coccus that N-1 profit is covered microbial inoculum;
Second step, linking agent preparation: take 7.5～8.0g boric acid, 3.5～4.0g Calcium Chloride Powder Anhydrous is dissolved in 200mL water, is made into the saturated boric acid solution of calcium chloride of desired concn, and regulate pH to 7～8 with sodium carbonate;
The 3rd step, microballoon preparation: the polyvinyl alcohol gel mixing is added drop-wise in the saturated boric acid solution of calcium chloride and is stirred, obtaining particle diameter is the active coccoid particle of immobilized microorganism of 3～5mm, is then at 4～8 DEG C in temperature, fixing crosslinked 24～36h, take out, use normal saline flushing.
2. the preparation method of the immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration according to claim 1, it is characterized in that: described attapulgite concentration is 0.5%, crosslinking time is 36h, PVA concentration is 8%, microbial inoculum concentration is 15%, calcium carbonate concentration is 0.4%, and silica concentration is 2.5%, and sodium alginate concentration is 0.5%.
3. the preparation method of the immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration according to claim 1, it is characterized in that: described attapulgite concentration is 0.5%, crosslinking time is 36h, microbial inoculum concentration is 15%, silica concentration is 3.5%, sodium alginate concentration is 1%, and calcium carbonate concentration is that 0.4%, PVA concentration is 8%.
4. according to the preparation method of the immobilized microorganism embedding microballoon for riverbed bed mud ecosystem restoration described in claim 1,2 or 3, it is characterized in that: the polyvinyl alcohol solution that is added with described embedding medium is stirred, be cooled to 20～25 DEG C and preserve 3～5h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310264883.2A CN103275963B (en) | 2013-06-27 | 2013-06-27 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310264883.2A CN103275963B (en) | 2013-06-27 | 2013-06-27 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103275963A CN103275963A (en) | 2013-09-04 |
| CN103275963B true CN103275963B (en) | 2014-11-19 |
Family
ID=49058584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310264883.2A Active CN103275963B (en) | 2013-06-27 | 2013-06-27 | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103275963B (en) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205412B (en) * | 2013-04-25 | 2016-04-13 | 西华大学 | The method of immobilized microorganism preparation and employing said preparation process meat product processing waste water |
| CN103523931A (en) * | 2013-10-25 | 2014-01-22 | 东南大学 | Method for repairing river-bed sediment ecological system by utilizing immobilized microorganism embedded microspheres |
| CN104278019A (en) * | 2013-11-15 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for mandarin fish culture water and preparation method thereof |
| CN103849615A (en) * | 2013-12-31 | 2014-06-11 | 浙江省海洋开发研究院 | Immobilized algal-bacterial symbiotic system for processing petroleum pollutants and application thereof |
| CN104671626B (en) * | 2015-02-02 | 2017-03-29 | 江苏省海洋水产研究所 | A kind of method of eutrophication Tidal Flat Sediments habitat rehabilitation |
| CN105523643B (en) * | 2015-12-25 | 2018-02-06 | 林立荣 | A kind of sewage disposal composite gel material of imbedded microbe and preparation method thereof |
| CN105754984B (en) * | 2016-04-13 | 2019-03-12 | 四川农业大学 | Compound immobilized microbial inoculum of sodium alginate and preparation method thereof and purposes |
| TWI602606B (en) * | 2016-05-30 | 2017-10-21 | 國立中興大學 | Contaminated sediment gel separation method |
| CN106636060A (en) * | 2017-01-26 | 2017-05-10 | 同济大学 | Microbial immobilization carrier, microbial immobilization equipment and microbial immobilization method |
| CN108328737B (en) * | 2017-11-30 | 2020-10-20 | 河海大学 | Micro-ecological substrate for strengthening treatment of aquaculture wastewater based on artificial wetland, preparation method and application thereof |
| CN108529759A (en) * | 2018-04-10 | 2018-09-14 | 北京华景山水生态科技有限公司 | A kind of water pollution sediment in-situ is biological reinforced to be handled and Benthic ecology restorative procedure |
| CN110577429A (en) * | 2018-06-08 | 2019-12-17 | 广东唐博生物科技有限公司 | double-layer embedded composite microbial fertilizer and preparation method thereof |
| CN109609406A (en) * | 2018-12-27 | 2019-04-12 | 广州市金龙峰环保设备工程股份有限公司 | A kind of slow-release microbial bacterial agent and preparation method thereof administered for black and odorous water |
| CN112441713B (en) * | 2019-08-23 | 2022-09-20 | 清上(苏州)环境科技有限公司 | In-situ remediation composite material and in-situ treatment and remediation method for polluted bottom mud |
| CN110563292B (en) * | 2019-09-11 | 2022-03-01 | 海绵城市投资有限公司 | Riverway bottom mud repairing agent and preparation and application methods thereof |
| CN111470614B (en) * | 2020-05-06 | 2022-03-29 | 北京工业大学 | Preparation and application of high-accumulation-rate short-cut nitrifying composite bacteria embedded bioactive filler |
| CN111892156B (en) * | 2020-07-03 | 2022-05-03 | 广州博嵩生物环保科技有限公司 | Porous degradable material and preparation method and application thereof |
| CN112723530A (en) * | 2020-12-11 | 2021-04-30 | 温州大学 | Preparation method of immobilized composite filler |
| CN114108319B (en) * | 2021-12-03 | 2023-09-22 | 山西大学 | Preparation method and application of gel fiber embedded with Shewanella |
| CN114275877A (en) * | 2021-12-30 | 2022-04-05 | 青岛威羽山环保科技有限公司 | Novel soft filler and preparation process thereof |
| CN114890841A (en) * | 2022-03-24 | 2022-08-12 | 武汉绿农瑞益生物科技有限公司 | Long-acting microbial compound fertilizer and preparation method thereof |
| CN114853153B (en) * | 2022-04-07 | 2023-12-15 | 航天凯天环保科技股份有限公司 | Microorganism-combined PRB repair system and application thereof in-situ repair of antibiotic-polluted groundwater |
| CN114774402A (en) * | 2022-04-19 | 2022-07-22 | 中交上海航道勘察设计研究院有限公司 | Method for cultivating and immobilizing microorganisms |
| CN114933509A (en) * | 2022-05-09 | 2022-08-23 | 武汉绿农瑞益生物科技有限公司 | Slow-release compound fertilizer and preparation method thereof |
| CN115708491B (en) * | 2022-11-16 | 2024-02-20 | 江苏海洋大学 | Culture substrate of buried shellfish and application of culture substrate in buried shellfish culture |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001299340A (en) * | 2000-04-26 | 2001-10-30 | Kansai Paint Co Ltd | Method for regulating specific gravity of enzyme or microbial immobilized carrier |
| CN102079571A (en) * | 2010-11-22 | 2011-06-01 | 兰州交通大学 | Nano attapulgite composite polyvinyl acetal foam microorganism immobilized carrier |
| CN103114085B (en) * | 2013-01-18 | 2015-10-21 | 西安建筑科技大学 | The method of SRB in the preparation of SRB biostats and crude oil for inhibiting |
-
2013
- 2013-06-27 CN CN201310264883.2A patent/CN103275963B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103275963A (en) | 2013-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103275963B (en) | Preparation method of immobilized microorganism embedding microsphere for restoring riverbed bottom mud ecosystem | |
| CN103224284B (en) | Rapid starting method of membrane bioreactor completely-autotrophic nitrogen removal technology | |
| CN103013872B (en) | Heterotrophic nitrifying and aerobic denitrifying bacterium and culture and application thereof | |
| CN105861479A (en) | Method for co-immobilizing anaerobic ammoxidation bacteria-short-cut nitrobacteria and application thereof | |
| CN101402485B (en) | Cohesive action nutrient source SRB sewage sludge immobilization particle, production and uses in treating heavy metal wastewater thereof | |
| Hatamoto et al. | Characterization of downflow hanging sponge reactors with regard to structure, process function, and microbial community compositions | |
| CN105858880B (en) | A kind of method of the coupling of immobilization Anammox short-cut denitrification Treating Municipal Sewage and nitrate wastewater | |
| Han et al. | Enhanced nitrogen removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine wastewater | |
| Tan et al. | Effects of tourmaline on nitrogen removal performance and biofilm structures in the sequencing batch biofilm reactor | |
| Wang et al. | Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis | |
| CN103496788A (en) | Rapid starting method of shortcut nitrification and denitrification in A/O (Anoxic/Oxic) process | |
| CN109081426B (en) | Microbial fuel cell type composite artificial wetland with low internal resistance | |
| CN110699347B (en) | Immobilized microbial inoculum and preparation method and application thereof | |
| Chi et al. | Nitrogen removal performance and bacterial flora analysis of a partial nitrification-anaerobic ammonium oxidation-denitrification system treating rare earth element tailings wastewater | |
| CN105152327A (en) | Culture method of anammox granule sludge with hydroxyapatite core | |
| CN107162187A (en) | A kind of preparation method of immobilization Anammox bacterium mud and its method for sewage disposal | |
| Kocaturk et al. | Investigation of the use of aerobic granules for the treatment of sugar beet processing wastewater | |
| CN109650543B (en) | SPNA integrated denitrification method for treating low-matrix wastewater under continuous flow condition | |
| Feng et al. | Nitrogen removal characteristics in a biofilm system for recirculating aquaculture wastewater treatment under high-salinity conditions and oligotrophic stress | |
| Di et al. | Experiment on the treatment of acid mine drainage with optimized biomedical stone particles by response surface methodology | |
| CN104445613A (en) | Mineralized refuse packed bed and wastewater denitrification method | |
| CN103991951A (en) | Embedded granule and method for removing estradiol in domestic wastewater by using embedded granule | |
| CN103805590A (en) | Immobilized low-temperature nitrobacterium and application thereof in treating low-temperature ammonia-nitrogen wastewater | |
| Muñoz-Palazón et al. | Simultaneous Nitrification and Denitrification Processes in Granular Sludge Technology | |
| Meng et al. | Influence of aeration rate on shortcut nitrification in an SBR treating anaerobic-digested piggery wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |