CN105858880A - Method for treating municipal sewage and nitrate wastewater by immobilized anaerobic ammoxidation coupled short-range denitrification - Google Patents

Method for treating municipal sewage and nitrate wastewater by immobilized anaerobic ammoxidation coupled short-range denitrification Download PDF

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Publication number
CN105858880A
CN105858880A CN201610265002.2A CN201610265002A CN105858880A CN 105858880 A CN105858880 A CN 105858880A CN 201610265002 A CN201610265002 A CN 201610265002A CN 105858880 A CN105858880 A CN 105858880A
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short
anammox
municipal sewage
cut denitrification
anaerobic ammoxidation
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CN105858880B (en
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王淑莹
韩金浩
贾方旭
张向晖
彭永臻
王众
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Beijing University of Technology
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/28Anaerobic digestion processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341Consortia of bacteria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention relates to a method for treating municipal sewage and nitrate wastewater by immobilized anaerobic ammoxidation coupled short-range denitrification, belonging to the technical field of sewage treatment. Polyvinyl alcohol and sodium alginate are used as embedding materials to perform anaerobic ammoxidation cell immobilization, and the prepared anaerobic ammoxidation gel pills are used in an SBR (sequencing batch reactor) system for treating municipal sewage and nitrate wastewater by anaerobic ammoxidation-short-range denitrification. The process unit comprises a municipal sewage raw water tank, a nitrate wastewater tank and an anaerobic ammoxidation-short-range denitrification SBR reactor. The immobilized microbe technique is utilized to solve the problems of high loss tendency, unstable systems operation and the like caused by poor settleability of the anaerobic ammoxidation bacteria. The immobilized anaerobic ammoxidation gel pills and short-range denitrification sludge coexist in the SBR system; and since the mass transfer between organic substances and toxic substances is hindered, the anaerobic ammoxidation bacteria in the immobilized pills can not be easily inhibited, thereby enhancing the treatment process stability of the system.

Description

A kind of immobilization Anammox coupling short-cut denitrification Treating Municipal Sewage and the method for nitrate wastewater
Technical field
The present invention relates to a kind of immobilization Anammox coupling short-cut denitrification Treating Municipal Sewage and the side of nitrate wastewater Method, belongs to technical field of sewage.Particular content is to utilize immobilized microorganism technique by highly active anaerobic ammonia oxidizing bacteria Be fixed in gelled pill, after including the gelled pill activity recovery of anaerobic ammonia oxidizing bacteria with there is high nitrite accumulation rate Short-cut denitrification mud co-inoculation enters reaction by a certain percentage in same sbr reactor device, municipal sewage and nitrate wastewater Device, the nitrate in nitrate wastewater is nitrite by short-cut denitrification Bacterial Transformation, in nitrite and municipal sewage Ammonia nitrogen is converted into nitrogen through anaerobic ammonium oxidation process, it is achieved the synchronization of ammonia nitrogen and nitrate is removed.
Background technology
Due to the quickening of China's industrial process, make body eutrophication not without the discharge of the strict Nitrogen-and Phosphorus-containing waste water processed in a large number Disconnected generation, water environment is seriously damaged.At present, nitrate pollution problem has caused people to pay high attention to, municipal sewage, The advanced nitrogen of industrial wastewater is treated as the study hotspot of current sewage disposal.
Anammox (anaerobic ammonium oxidation, Anammox) is to be currently known economy, efficient Biological denitrificaion new way, it is possible to effectively solve that the investment that traditional nitrification-denitrification denitrogenation causes is high, power consumption is big, be unsuitable for The problem that high nitrogen low-carbon (LC) waste water processes, has a good application prospect in denitrogenation of waste water field.Anammox reaction is in anoxic Under the conditions of with NH4 +For electron donor, NO2 -For electron acceptor, the two is transformed into N2Denitrification of autotrophic organism process.Its Central Asia Nitrate is the necessary matrix that reaction is required, and the approach being commonly available nitrite is short distance nitration, but in low temperature, low-carbon and nitrogen Be difficult to realization in the city domestic sewage of ratio, nitrous accumulation rate is difficult to remain stable.In addition nitre can be produced during Anammox Hydrochlorate, increasingly strict along with discharge standard of municipal sewage plant, this part nitrate can cause the sewage after processing not reach row Put standard.Short-cut denitrification process is the another approach obtaining nitrite, and nitrate by short-cut denitrification Bacterial Transformation is Nitrite, course of reaction controls simple, it is possible to steady in a long-term realize higher nitrite accumulation, and can be by The nitrate removal that Anammox process produces.Therefore Anammox and short-cut denitrification be integrated as city domestic sewage and The process of high concentration nitrate waste water provides new way.But owing to anaerobic ammonia oxidizing bacteria poor growth, growth rate are low, from generation to generation Cycle is long, and activity is easily subject to the impact of temperature, pH, dissolved oxygen etc., and a large amount of nitrogen produced in course of reaction make mud hang Floating in reactor, settling property is poor, easily causes thalline and runs off, and causes system to be difficult to remain stable.
In order to solve the problems referred to above, it is little that the anaerobic ammonia oxidizing bacteria being easy to run off is made gel by the method that the present invention uses embedding fixing Ball is trapped in Anammox-short-cut denitrification sbr reactor device.Polyvinyl alcohol and sodium alginate, both are hydrophily Polymer intersolubility good, in conventional research, bi-material is typically to be used alone, and fails to combine their advantage, Undesirable, by high intensity and the high-moisture percentage phase of calcium alginate of polyvinyl alcohol at the aspect of performance such as the elasticity of material and moisture content In conjunction with, prepare and have that high resiliency, high-flexibility be big and the Anammox gelled pill of high-moisture percentage.Using PVA-SA as bag Bury fixation support prepare Anammox gelled pill and applied in Anammox-short-cut denitrification process city dirty In the SBR system of water and nitrate wastewater, it is possible to solve the problem that anaerobic ammonia oxidizing bacteria runs off.Embedded immobilization microbial obtains The gelled pill biomass concentration obtained is high, mechanical strength is big, settling property good, it is possible to maintain the anti-nitre of Anammox-short distance The stable operation of change system.
Summary of the invention
The invention aims to solve the anaerobism that anaerobic ammonia oxidizing bacteria runs off, organic substance influence anaerobic ammoxidation activity causes The problems such as the SBR system fluctuation of service of ammoxidation-short-cut denitrification Treating Municipal Sewage and nitrate wastewater, utilize fixing Change microbial technique and prepare Anammox gelled pill, anaerobic ammonia oxidizing bacteria retained in systems and weaken organic matter to detesting The impact of anaerobic ammonium oxidation bacterium, strengthens system run all right.
In order to achieve the above object, the present invention is realized by below scheme:
One, the preparation of Anammox immobilized spherule
(1) bacterial classification: take the anaerobic ammoxidation activity mud that sludge concentration is 9000ml/L, by the deionized water after deoxygenation After cleaning standby.
(2) preparation of embedding medium: according to the quality-concentration expressed in percentage by volume preparation 200ml polyvinyl alcohol of 15g/L and 2g/L (PVA) and sodium alginate (SA) mixed solution 1., and in 120 DEG C heat 20min make its accelerate dissolve;According to 50g/L Quality-concentration expressed in percentage by volume preparation 500ml sodium nitrate (NaNO with 2g/L3) and calcium chloride (CaCl2) mixing fix Liquid is 2..
(3) embedding of anaerobic ammonia oxidizing bacteria is fixed: the Anammox bacterial classification and (2nd) step that (1st) step are obtained obtain To mixed solution take 25ml the most respectively, obtain bacterium-glue mixed liquor after being sufficiently mixed, be then added dropwise to 250ml and mix Close fixer 2. in, be at room temperature fully contacted 12h and its immobilization cross-linked, spend the deionized water after deoxygenation after terminating clear Wash immobilized spherule 3-5 time.
(4) activation: use ammonia nitrogen and nitrous dense under conditions of 30-35 DEG C and pH=7.5 the immobilized spherule prepared Degree is respectively water distribution activation culture 6-8 days of 30mg/L, makes the microbial activity within immobilized spherule be restored.
Action principle is:
It is good that polyvinyl alcohol and sodium alginate are hydrophilic polymer, intersolubility, and being used in combination of PVA and SA combines two The advantage that person's mechanical strength is high and moisture content is high has prepared that high resiliency, high-flexibility be big and the Anammox gel of high-moisture percentage Bead, after overactivation, gelled pill activity is restored.
Two, the Anammox gelled pill obtained by embedded immobilization is applied and is processed city at Anammox-short-cut denitrification In the SBR system of city's sewage and nitrate wastewater, its device characteristic is:
This system is by the former water tank of municipal sewage, nitrate wastewater water tank and Anammox-short-cut denitrification sbr reactor device It is formed by connecting;The former water tank of municipal sewage is by the first intake pump and Anammox-short-cut denitrification sbr reactor device first Water inlet pipe is connected, and Anammox-short-cut denitrification sbr reactor device is provided with agitator, atmospheric valve and outlet valve;Nitrate gives up Water tank is connected with Anammox-short-cut denitrification reactor the second water inlet pipe by the second intake pump.
Three, the Anammox gelled pill utilizing embedded immobilization to obtain realizes Anammox-short-cut denitrification and processes city City's sewage and the method for nitrate wastewater, comprise the following steps:
Step one: inoculation nitrous accumulation rate is that the short-cut denitrification mud of 80%-100% is in Anammox-short-cut denitrification In sbr reactor device, after inoculation, sludge concentration is 2500-3000mg/L.Then immobilized Anammox gel is inoculated little Ball, immobilized spherule packing ratio is 20%-25%.
Step 2: the municipal sewage in the former water tank of municipal sewage is by the first intake pump and the nitre in nitrate wastewater water tank Hydrochlorate waste water enters into Anammox-short-cut denitrification sbr reactor for 7:1-12:1 by volume by the second intake pump Device;Anoxia stirring 8-10h after water inlet;Precipitation 30-40min, gets rid of supernatant according to the draining ratio of 40%-50%.
The present invention compared with prior art has the advantage that
(1) above-mentioned embedding method is used can to retain the activity of fixing rear anaerobic ammonia oxidizing bacteria to greatest extent and not run off.
(2) Anammox-short-cut denitrification Treating Municipal Sewage and nitre that Anammox immobilization technology starts are utilized Hydrochlorate waste water system, it is possible to maintain stable operation.
(3) Anammox-short-cut denitrification Treating Municipal Sewage and nitre that Anammox immobilization technology starts are utilized In hydrochlorate waste water system, sludge settling property is good.
(4) utilizing short-cut denitrification mud is nitrite by the nitrate reduction in nitrate wastewater, for Anammox Stable response matrix is provided.
Accompanying drawing explanation
Fig. 1 is that embedded immobilization anaerobic ammonium oxidizing bacteria is at Anammox-short-cut denitrification Treating Municipal Sewage and nitrate Schematic diagram in waste water sbr reactor device.
Fig. 2 is Anammox-short-cut denitrification Treating Municipal Sewage and nitrate wastewater device schematic diagram.
In Fig. 2: the former water tank-1 of municipal sewage, nitrate wastewater water tank the-2, first intake pump-3, the second intake pump-4, Anammox-short-cut denitrification sbr reactor device-5, agitator-6, atmospheric valve-7, the first outlet valve-8, the second water outlet Valve-9.
Detailed description of the invention
Below in conjunction with embodiment, the present invention done the most concrete description, but embodiments of the present invention are not limited to this.
Embodiment 1:
The preparation of Anammox gelled pill, detailed process is as follows:
Choose the anaerobic ammonium oxidation sludge that sludge concentration is 9000mg/L, standby after cleaning by the deionized water after deoxygenation.Bag Bury the preparation of agent: according to quality-concentration expressed in percentage by volume preparation 200ml polyvinyl alcohol (PVA) and the sea of 15g/L and 2g/L Mosanom (SA) mixed solution 1., and in 120 DEG C heat 20min make its accelerate dissolve;According to 50g/L and 2g/L Quality-concentration expressed in percentage by volume preparation 500ml sodium nitrate (NaNO3) and calcium chloride (CaCl2) mixed stationary liquid 2..Dense Anaerobic ammonium oxidation sludge and mixed solution that degree is 9000mg/L take 25ml the most respectively, obtain bacterium-glue mixed liquor after being sufficiently mixed, Then it is added dropwise to 250ml mixed stationary liquid 2. middle relative quantity, is at room temperature fully contacted 12h and its immobilization is cross-linked, Immobilized spherule is cleaned 3-5 time by the deionized water of deoxygenation after end.By the immobilized spherule for preparing at 30-35 DEG C and It is respectively water distribution activation culture 6-8 days of 30mg/L by ammonia nitrogen and nitrous concentration under conditions of pH=7.5, in making immobilized spherule The microbial activity in portion is restored.Test result indicate that the Anammox gelled pill after fixing and loose anaerobism ammonia Oxidation activity is identical.
Embodiment 2:
Utilizing Anammox immobilization technology coupling short-cut denitrification Treating Municipal Sewage and nitrate wastewater, detailed process is such as Under:
As it is shown in figure 1, the Anammox bead by volume filling rate 20% after above-mentioned activation is added to Anammox coupling Close in the sbr reactor device (5) of short-cut denitrification Treating Municipal Sewage and nitrate wastewater, reactor dischargeable capacity 10L;Connect Kind of nitrous accumulation rate be the short-cut denitrification mud of 80%-100% in Anammox-short-cut denitrification sbr reactor device (5), After inoculation, sludge concentration is 2500-3000mg/L;Municipal sewage in the former water tank of municipal sewage (1) passes through the first intake pump (3) entered for 10:1 by volume by the second intake pump (4) with the nitrate wastewater in nitrate wastewater water tank (2) To Anammox-short-cut denitrification sbr reactor device;Anoxia stirring 10h after water inlet;Precipitation 40min, according to the row of 40% Water is than getting rid of supernatant.
Test result indicate that, stable rear Anammox coupling short-cut denitrification Treating Municipal Sewage and nitrate wastewater Sbr reactor device effluent COD concentration is 40-6mg/L, NH+ 4-N concentration < 5mg/L, NO- 2-N concentration < 5mg/L, NO- 3-N Concentration < 5mg/L, water outlet TN < 15mg/L, reach discharge standard.

Claims (2)

1. an immobilization Anammox coupling short-cut denitrification Treating Municipal Sewage and nitric acid The device of salt waste water, it is characterised in that:
This system is by the former water tank of municipal sewage (1), nitrate wastewater water tank (2) and anaerobism Ammoxidation-short-cut denitrification sbr reactor device (5) is formed by connecting;The former water tank of municipal sewage (1) By the first intake pump (3) and Anammox-short-cut denitrification sbr reactor device (5) the One water inlet pipe be connected, Anammox-short-cut denitrification sbr reactor device be provided with agitator (6), Atmospheric valve (7), the first outlet valve (8) and the second outlet valve (9);Nitrate wastewater water tank (2) by the second intake pump (4) and Anammox-short-cut denitrification reactor (5) the Two water inlet pipes are connected.
2. an immobilization Anammox coupling short-cut denitrification Treating Municipal Sewage and nitric acid The method of salt waste water, comprises the following steps:
Step one: inoculation nitrous accumulation rate is that the short-cut denitrification mud of 80%-100% is in anaerobism In ammoxidation-short-cut denitrification sbr reactor device (5), after inoculation, sludge concentration is 2500-3000mg/L;Then the Anammox gelled pill that inoculation immobilization obtains, fixing Changing bead packing ratio is 20%-25%;
Step 2: the municipal sewage in the former water tank of municipal sewage (1) passes through the first intake pump (3) nitrate wastewater with concentration 300-500mg/L in nitrate wastewater water tank (2) leads to Crossing the second intake pump (4), to enter into Anammox-short distance for 7:1-12:1 by volume anti- Nitrification sbr reactor device (5);Anoxia stirring 8-10h after water inlet;Precipitation 30-40min, according to The draining of 40%-50% is than getting rid of supernatant.
CN201610265002.2A 2016-04-25 2016-04-25 A kind of method of the coupling of immobilization Anammox short-cut denitrification Treating Municipal Sewage and nitrate wastewater Active CN105858880B (en)

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106430572A (en) * 2016-11-28 2017-02-22 北京工业大学 Method and device for joint denitrifying by denitrification and anaerobic ammonia oxidation embedding particles
CN110526528A (en) * 2019-10-10 2019-12-03 苏州科技大学 Integral type short-cut denitrification Anammox water process quick start method and system
CN111423051A (en) * 2020-03-09 2020-07-17 广东工业大学 Composite particle with short-cut denitrification coupling anaerobic ammonia oxidation and preparation method and application thereof
WO2021035806A1 (en) * 2019-08-29 2021-03-04 南京大学 Method for stating integrated denitrification system combining short-term denitrification and anammox
CN112759085A (en) * 2021-03-04 2021-05-07 苏州绿业环境发展有限公司 Method for synergistic denitrification of short-range denitrifying bacteria-dissimilatory nitrate reducing bacteria-short-range nitrifying bacteria-anaerobic ammonium oxidizing bacteria
CN112897691A (en) * 2021-03-04 2021-06-04 苏州绿业环境发展有限公司 Method for treating wastewater by using short-range denitrifying bacteria and anaerobic ammonium oxidation bacteria based on immobilization
CN113716686A (en) * 2021-08-24 2021-11-30 北京工业大学 Device and method for quickly starting deep denitrification of landfill leachate by coupling short-cut denitrification with anaerobic ammonia oxidation
CN114506923A (en) * 2022-01-12 2022-05-17 西安建筑科技大学 Biological denitrification device and method for coupling synchronous PD activated sludge with Anammox biological membrane
CN114804517A (en) * 2022-04-16 2022-07-29 江苏裕隆环保有限公司 EGA sewage treatment method with pre-buried anaerobic ammonium oxidation bacteria biological suspension filler
CN116102163A (en) * 2022-09-07 2023-05-12 北京工业大学 Device and method for enriching anaerobic ammonia oxidation bacteria in short-cut denitrification particles to strengthen denitrification of domestic sewage

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CN102515350A (en) * 2011-12-09 2012-06-27 北京工业大学 Apparatus and method for nitrogen removal by combining garbage leachate SBR and anaerobic ammoxidation
CN104829044A (en) * 2015-04-18 2015-08-12 北京工业大学 Method used for realizing whole-course autotrophic nitrogen removing via anaerobic ammonium oxidation immobilization

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KR20020072360A (en) * 2001-03-09 2002-09-14 주식회사 제일엔지니어링 Biological Nitrogen Removal from Nitrogen-Rich Wastewaters by Partial Nitrification and Anaerobic Ammonium Oxidation
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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106430572B (en) * 2016-11-28 2023-12-15 北京工业大学 Method and device for combined denitrification by using denitrification and anaerobic ammonia oxidation embedded particles
CN106430572A (en) * 2016-11-28 2017-02-22 北京工业大学 Method and device for joint denitrifying by denitrification and anaerobic ammonia oxidation embedding particles
WO2021035806A1 (en) * 2019-08-29 2021-03-04 南京大学 Method for stating integrated denitrification system combining short-term denitrification and anammox
CN110526528A (en) * 2019-10-10 2019-12-03 苏州科技大学 Integral type short-cut denitrification Anammox water process quick start method and system
CN111423051A (en) * 2020-03-09 2020-07-17 广东工业大学 Composite particle with short-cut denitrification coupling anaerobic ammonia oxidation and preparation method and application thereof
CN112759085A (en) * 2021-03-04 2021-05-07 苏州绿业环境发展有限公司 Method for synergistic denitrification of short-range denitrifying bacteria-dissimilatory nitrate reducing bacteria-short-range nitrifying bacteria-anaerobic ammonium oxidizing bacteria
CN112897691A (en) * 2021-03-04 2021-06-04 苏州绿业环境发展有限公司 Method for treating wastewater by using short-range denitrifying bacteria and anaerobic ammonium oxidation bacteria based on immobilization
CN113716686A (en) * 2021-08-24 2021-11-30 北京工业大学 Device and method for quickly starting deep denitrification of landfill leachate by coupling short-cut denitrification with anaerobic ammonia oxidation
CN113716686B (en) * 2021-08-24 2023-03-14 北京工业大学 Method for rapidly starting deep denitrification of landfill leachate by coupling short-cut denitrification with anaerobic ammonia oxidation
CN114506923A (en) * 2022-01-12 2022-05-17 西安建筑科技大学 Biological denitrification device and method for coupling synchronous PD activated sludge with Anammox biological membrane
CN114804517A (en) * 2022-04-16 2022-07-29 江苏裕隆环保有限公司 EGA sewage treatment method with pre-buried anaerobic ammonium oxidation bacteria biological suspension filler
CN116102163A (en) * 2022-09-07 2023-05-12 北京工业大学 Device and method for enriching anaerobic ammonia oxidation bacteria in short-cut denitrification particles to strengthen denitrification of domestic sewage
CN116102163B (en) * 2022-09-07 2024-04-26 北京工业大学 Device and method for enriching anaerobic ammonia oxidation bacteria in short-cut denitrification particles to strengthen denitrification of domestic sewage

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