CN105858880B - A kind of method of the coupling of immobilization Anammox short-cut denitrification Treating Municipal Sewage and nitrate wastewater - Google Patents
A kind of method of the coupling of immobilization Anammox short-cut denitrification Treating Municipal Sewage and nitrate wastewater Download PDFInfo
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- CN105858880B CN105858880B CN201610265002.2A CN201610265002A CN105858880B CN 105858880 B CN105858880 B CN 105858880B CN 201610265002 A CN201610265002 A CN 201610265002A CN 105858880 B CN105858880 B CN 105858880B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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Abstract
A kind of immobilization Anammox coupling short-cut denitrification Treating Municipal Sewage and the method for nitrate wastewater belong to technical field of sewage.The present invention chooses polyvinyl alcohol and sodium alginate as embedding medium and carries out Anammox cell fixation, the Anammox gelled pill being prepared is applied in the SBR system of Anammox-short-cut denitrification Treating Municipal Sewage and nitrate wastewater.Process unit includes municipal sewage raw water water tank, nitrate wastewater water tank and Anammox-short-cut denitrification sbr reactor device.The present invention solves the problems such as being easy to run off caused by anaerobic ammonia oxidizing bacteria settling property difference, system fluctuation of service using immobilized microorganism technique.The Anammox gelled pill and short-cut denitrification sludge of immobilization coexist in SBR system, and since organic matter and noxious material mass transfer are obstructed, the anaerobic ammonia oxidizing bacteria in immobilized spherule is not easily susceptible to inhibit, and enhances the stability of the system treatment process.
Description
Technical field
The present invention relates to a kind of immobilization Anammox coupling short-cut denitrification Treating Municipal Sewage and nitrate wastewaters
Method, belong to technical field of sewage.Particular content is to utilize immobilized microorganism technique by the anaerobism ammonia oxygen of high activity
Change bacterium to be fixed in gelled pill, include after the gelled pill activity recovery of anaerobic ammonia oxidizing bacteria with there is high nitrite accumulation
The short-cut denitrification sludge co-inoculation of rate enters by a certain percentage in same sbr reactor device, municipal sewage and nitrate wastewater
Reactor, the nitrate in nitrate wastewater are converted into nitrite, nitrite and municipal sewage by short-cut denitrification bacterium
In ammonia nitrogen be converted into nitrogen by anaerobic ammonium oxidation process, realize that ammonia nitrogen is synchronous with nitrate and remove.
Background technique
Due to the quickening of China's industrial process, the discharge largely without the Nitrogen-and Phosphorus-containing waste water strictly handled makes water eutrophication
Change and constantly occur, water environment is seriously damaged.Currently, nitrate pollution problem has caused people to pay high attention to, municipal sewage,
The advanced nitrogen of industrial wastewater is treated as the research hotspot of current sewage treatment.
Anammox (anaerobic ammonium oxidation, Anammox) be currently known it is economical, efficient
Biological denitrificaion new way can effectively solve the problem that investment is high caused by traditional nitrification-denitrification denitrogenation, energy consumption is big, be unsuitable for height
The problem of nitrogen low-carbon wastewater treatment, has a good application prospect in denitrogenation of waste water field.Anammox reaction is in anoxia condition
Under with NH4 +For electron donor, NO2 -For electron acceptor, the two is transformed into N2Denitrification of autotrophic organism process.Wherein nitrite
It is the required necessary matrix of reaction, the approach for being commonly available nitrite is short distance nitration, but in the city of low temperature, low ratio of carbon to ammonium
It is not easy to realize in sanitary sewage, nitrous accumulation rate is difficult to remain stable.Furthermore nitrate can be generated during Anammox, with
Discharge standard of municipal sewage plant it is increasingly stringent, this part nitrate will lead to that treated, and discharge standard is not achieved in sewage.It is short
Journey denitrification process is the another approach for obtaining nitrite, and nitrate is converted into nitrite by short-cut denitrification bacterium,
Reaction process control is simple, can realize higher nitrite accumulation steadily in the long term, and can generate Anammox process
Nitrate removal.Therefore Anammox and short-cut denitrification are integrated as city domestic sewage and high concentration nitrate waste water
Processing provide new way.But due to anaerobic ammonia oxidizing bacteria slow growth, growth rate is low, the generation cycle is long, activity be easy by
To the influence of temperature, pH, dissolved oxygen etc., a large amount of nitrogen generated in reaction process make sludge suspension in reactor, settleability
Can be poor, it easily causes thallus to be lost, system is caused to be difficult to maintain to stablize.
To solve the above-mentioned problems, the present invention anaerobic ammonia oxidizing bacteria being easy to run off is made using the fixed method of embedding solidifying
Glue bead is trapped in Anammox-short-cut denitrification sbr reactor device.Polyvinyl alcohol and sodium alginate, the two are hydrophilic
The advantages of polymer intersolubility of property is good, and two kinds of materials are usually single use in previous research, fails them combines
Come, it is undesirable in the aspect of performance such as the elasticity of material and moisture content, by the high intensity of polyvinyl alcohol and the High water cut of calcium alginate
Rate combines, and being made has high resiliency, high-flexibility big and the Anammox gelled pill of high-moisture percentage.Using PVA-SA as packet
Fixation support is buried to prepare Anammox gelled pill and applied dirty in Anammox-short-cut denitrification processing city
In the SBR system of water and nitrate wastewater, it is able to solve the problem of anaerobic ammonia oxidizing bacteria is lost.Embedded immobilization microbial obtains
Gelled pill biomass concentration it is high, mechanical strength is big, settling property is good, be able to maintain that Anammox-short-cut denitrification system
The stable operation of system.
Summary of the invention
The purpose of the present invention is to solve anaerobic ammonia oxidizing bacterias to be lost, caused by organic substance influence anaerobic ammoxidation activity
The problems such as Anammox-short-cut denitrification Treating Municipal Sewage and the SBR system fluctuation of service of nitrate wastewater, using admittedly
Surely change microbial technique and prepare Anammox gelled pill, anaerobic ammonia oxidizing bacteria is retained in systems and weakens organic matter pair
The influence of anaerobic ammonia oxidizing bacteria enhances system run all right.
In order to achieve the above object, the present invention is realized by following scheme:
One, the preparation of Anammox immobilized spherule
(1) strain: taking sludge concentration is the anaerobic ammoxidation activity sludge of 9000ml/L, clear with the deionized water after deoxygenation
It washes rear spare.
(2) preparation of embedding medium: 200ml polyvinyl alcohol is prepared according to quality-concentration expressed in percentage by volume of 15g/L and 2g/L
(PVA) and sodium alginate (SA) mixed solution 1., and in 120 DEG C heat 20min make its accelerate dissolution;According to 50g/L and 2g/
The quality of L-concentration expressed in percentage by volume prepares 500ml sodium nitrate (NaNO3) and calcium chloride (CaCl2) mixed stationary liquid 2..
(3) embedding of anaerobic ammonia oxidizing bacteria is fixed: Anammox strain and (2) step that (1) step obtains are obtained
Mixed solution 1. take 25ml respectively, bacterium-glue mixed liquor is obtained after being sufficiently mixed, it is solid to be then added dropwise to 250ml mixing
Determine liquid 2. in, come into full contact with 12h at room temperature and its immobilization be crosslinked, after spend the cleaning of the deionized water after deoxygenation and fix
Change bead 3-5 times.
(4) activate: by the immobilized spherule prepared, ammonia nitrogen and nitrous are dense under conditions of 30-35 DEG C and pH=7.5
Degree is respectively water distribution activation culture 6-8 days of 30mg/L, and the microbial activity inside immobilized spherule is made to be restored.
Action principle is:
Polyvinyl alcohol and sodium alginate are that hydrophilic polymer, intersolubility are good, and PVA and being used in combination for SA combine
It is big solidifying with the Anammox of high-moisture percentage that high resiliency, high-flexibility has been made in the two high mechanical strength and the high advantage of moisture content
Glue bead, gelled pill activity is restored after overactivation.
Two, the Anammox gelled pill that embedded immobilization obtains is applied at Anammox-short-cut denitrification
In the SBR system for managing municipal sewage and nitrate wastewater, device characteristic is:
The system is by municipal sewage raw water water tank, nitrate wastewater water tank and Anammox-short-cut denitrification sbr reactor
Device is formed by connecting;Municipal sewage raw water water tank passes through the first intake pump and Anammox-short-cut denitrification sbr reactor device first
Water inlet pipe is connected, and Anammox-short-cut denitrification sbr reactor device is equipped with blender, blow valve and outlet valve;Nitrate wastewater
Water tank is connected by the second intake pump with Anammox-the second water inlet pipe of short-cut denitrification reactor.
Three, it is realized at Anammox-short-cut denitrification using the Anammox gelled pill that embedded immobilization obtains
The method for managing municipal sewage and nitrate wastewater, comprising the following steps:
Step 1: the short-cut denitrification sludge that inoculation nitrous accumulation rate is 80%-100% is anti-in Anammox-short distance
It nitrifies in sbr reactor device, sludge concentration is 2500-3000mg/L after inoculation.Then it is inoculated with the Anammox gel of immobilization
Bead, immobilized spherule packing ratio are 20%-25%.
Step 2: the municipal sewage in municipal sewage raw water water tank passes through in the first intake pump and nitrate wastewater water tank
Nitrate wastewater is that 7:1-12:1 enters Anammox-short-cut denitrification sbr reactor by the second intake pump by volume
Device;Anoxia stirring 8-10h after water inlet;30-40min is precipitated, according to the draining of 40%-50% than excluding supernatant.
Compared with the prior art, the present invention has the following advantages:
(1) activity of anaerobic ammonia oxidizing bacteria after fixing can be retained to greatest extent using above-mentioned embedding method and be not lost.
(2) using Anammox immobilization technology starting Anammox-short-cut denitrification Treating Municipal Sewage and
Nitrate wastewater system, is able to maintain that stable operation.
(3) using Anammox immobilization technology starting Anammox-short-cut denitrification Treating Municipal Sewage and
Sludge settling property is good in nitrate wastewater system.
(4) it is nitrite by the nitrate reduction in nitrate wastewater using short-cut denitrification sludge, is anaerobism ammonia oxygen
Change and stable response matrix is provided.
Detailed description of the invention
Fig. 1 is embedded immobilization anaerobic ammonium oxidizing bacteria in Anammox-short-cut denitrification Treating Municipal Sewage and nitre
Schematic diagram in hydrochlorate waste water sbr reactor device.
Fig. 2 is Anammox-short-cut denitrification Treating Municipal Sewage and nitrate wastewater schematic device.
In Fig. 2: municipal sewage raw water water tank -1, nitrate wastewater water tank -2, the first intake pump -3, the second intake pump -4,
Anammox-short-cut denitrification sbr reactor device -5, blender -6, blow valve -7, the first outlet valve -8, the second outlet valve -9.
Specific embodiment
Further specific description is done to the present invention below with reference to embodiment, embodiments of the present invention are not limited thereto.
Embodiment 1:
The preparation of Anammox gelled pill, detailed process is as follows:
The anaerobic ammonium oxidation sludge that sludge concentration is 9000mg/L is chosen, it is spare after being cleaned with the deionized water after deoxygenation.
The preparation of embedding medium: 200ml polyvinyl alcohol (PVA) and alginic acid are prepared according to quality-concentration expressed in percentage by volume of 15g/L and 2g/L
Sodium (SA) mixed solution 1., and in 120 DEG C heat 20min make its accelerate dissolution;According to quality-volume of 50g/L and 2g/L
Percentage concentration prepares 500ml sodium nitrate (NaNO3) and calcium chloride (CaCl2) mixed stationary liquid 2..Concentration is detesting for 9000mg/L
1. anaerobic ammonium oxidation sludge and mixed solution take 25ml respectively, bacterium-glue mixed liquor is obtained after being sufficiently mixed, is then added dropwise to
250ml mixed stationary liquid 2. in relative quantity, come into full contact at room temperature 12h to its immobilization be crosslinked, after gone with deoxygenation
Ionized water cleans immobilized spherule 3-5 times.By the immobilized spherule prepared at 30-35 DEG C and under conditions of pH=7.5 ammonia
Nitrogen and nitrous concentration are respectively water distribution activation culture 6-8 days of 30mg/L, obtain the microbial activity inside immobilized spherule extensive
It is multiple.The experimental results showed that the Anammox gelled pill after fixed is identical as loose anaerobic ammoxidation activity.
Embodiment 2:
Short-cut denitrification Treating Municipal Sewage and nitrate wastewater, specific mistake are coupled using Anammox immobilization technology
Journey is as follows:
As shown in Figure 1, the Anammox bead after above-mentioned activation is added to anaerobism ammonia oxygen by volumetric filling ratio 20%
In the sbr reactor device (5) for changing coupling short-cut denitrification Treating Municipal Sewage and nitrate wastewater, reactor dischargeable capacity 10L;It connects
Kind nitrous accumulation rate is the short-cut denitrification sludge of 80%-100% in Anammox-short-cut denitrification sbr reactor device (5)
In, sludge concentration is 2500-3000mg/L after inoculation;Municipal sewage in municipal sewage raw water water tank (1) passes through the first water inlet
Pump (3), which is entered by volume for 10:1 with the nitrate wastewater in nitrate wastewater water tank (2) by the second intake pump (4), to be detested
Anaerobic ammonium oxidation-short-cut denitrification sbr reactor device;Anoxia stirring 10h after water inlet;40min is precipitated, according to 40% draining than excluding
Supernatant.
The experimental results showed that Anammox coupling short-cut denitrification Treating Municipal Sewage and nitrate are useless after stable
The sbr reactor device effluent COD concentration of water is 40-6mg/L, NH+ 4- N concentration < 5mg/L, NO- 2- N concentration < 5mg/L, NO- 3- N is dense
< 5mg/L is spent, TN < 15mg/L is discharged, reaches discharge standard.
Claims (1)
1. a kind of method of the coupling of immobilization Anammox short-cut denitrification Treating Municipal Sewage and nitrate wastewater, using such as
Lower system:
The system is anti-by municipal sewage raw water water tank (1), nitrate wastewater water tank (2) and Anammox-short-cut denitrification SBR
Device (5) is answered to be formed by connecting;Municipal sewage raw water water tank (1) passes through the first intake pump (3) and Anammox-short-cut denitrification
(5) first water inlet pipe of sbr reactor device is connected, and Anammox-short-cut denitrification sbr reactor device is equipped with blender (6), blow valve
(7), the first outlet valve (8) and the second outlet valve (9);Nitrate wastewater water tank (2) passes through the second intake pump (4) and anaerobism ammonia oxygen
(5) second water inlet pipe of change-short-cut denitrification reactor is connected;
Characterized by comprising the following steps:
Step 1: inoculation nitrous accumulation rate is the short-cut denitrification sludge of 80%-100% in Anammox-short-cut denitrification
In sbr reactor device (5), sludge concentration is 2500-3000mg/L after inoculation;Then the Anammox that inoculation immobilization obtains is solidifying
Glue bead, immobilized spherule packing ratio are 20%-25%;
Step 2: the municipal sewage in municipal sewage raw water water tank (1) passes through the first intake pump (3) and nitrate wastewater water tank
(2) nitrate wastewater of the concentration 300-500mg/L in is entered by volume for 7:1-12:1 by the second intake pump (4) to be detested
Anaerobic ammonium oxidation-short-cut denitrification sbr reactor device (5);Anoxia stirring 8-10h after water inlet;30-40min is precipitated, according to 40%-
50% draining is than excluding supernatant.
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CN112759085A (en) * | 2021-03-04 | 2021-05-07 | 苏州绿业环境发展有限公司 | Method for synergistic denitrification of short-range denitrifying bacteria-dissimilatory nitrate reducing bacteria-short-range nitrifying bacteria-anaerobic ammonium oxidizing bacteria |
CN112897691A (en) * | 2021-03-04 | 2021-06-04 | 苏州绿业环境发展有限公司 | Method for treating wastewater by using short-range denitrifying bacteria and anaerobic ammonium oxidation bacteria based on immobilization |
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KR20020072360A (en) * | 2001-03-09 | 2002-09-14 | 주식회사 제일엔지니어링 | Biological Nitrogen Removal from Nitrogen-Rich Wastewaters by Partial Nitrification and Anaerobic Ammonium Oxidation |
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KR20020072360A (en) * | 2001-03-09 | 2002-09-14 | 주식회사 제일엔지니어링 | Biological Nitrogen Removal from Nitrogen-Rich Wastewaters by Partial Nitrification and Anaerobic Ammonium Oxidation |
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