CN104784112A - Method for inducing settlement and metamorphosis of capitulum mitella linnaeus larvae with juvenile hormone-III - Google Patents
Method for inducing settlement and metamorphosis of capitulum mitella linnaeus larvae with juvenile hormone-III Download PDFInfo
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Abstract
The invention relates to the technical field of aquaculture, in particular to a preparation and an application method for inducing settlement and metamorphosis of capitulum mitella linnaeus larvae with juvenile hormone-III. The invention adopts the technical scheme that a preparation for inducing the settlement and the metamorphosis of the capitulum mitella linnaeus larvae is prepared in a water body by the following steps of carrying out solubilization with dimethyl sulfoxide according to a weight-to-volume ratio of the juvenile hormone-III to dimethyl sulfoxide as 1/25 (w/v), adding alcohol to prepare the preparation containing 0.1mg/mL juvenile hormone -III, and storing at -20 DEG C. Filtered sea water is added to prepare inducing sea water before use. The preparation is simple to operate, low in cost, stable in action and significant in inducing effect, and a metamorphosis rate can reach 86%. The method presents appropriate concentration, treatment time and inducing opportunity for application of the preparation and is a good method for inducing the settlement and the metamorphosis of the capitulum mitella linnaeus larvae, and the metamorphosis rate of the larvae is up to 95%.
Description
Technical field
The present invention relates to the technical field of aquaculture, be specifically related to a kind of preparation and the application process that utilize juvenile hormone-III induction Testudinis foot cyprids settlement and metamorphosis.
Background technology
Testudinis foot (
capitulum mitellalinnaeus) be under the jurisdiction of Arthropoda (Arthropoda) carapace subphylum (Crustacea) cirrus infraclass (Cirripedia) enclose breast catalogue (Thoracica) have handle order (Pedunculata) armour Lepadomorpha (Scalpellomorpha) Zhi Ming lotus section (Pollicipidae) Testudinis foot belong to (
capitulum), gain the name because of the similar Testudinis foot of its bodily form, have another name called barnacle, Fructus Citri Sarcodactylis, Testudinis foot, penholder etc.Mainly be distributed in the rock slot of Intertidal zone, Perenniporia martius marine site.Testudinis foot has higher nutritive value and medical value, Testudinis foot on domestic market is all rely on natural sea area to adopt to catch, and has certain difficulty, and causes certain destruction to the habitat of Testudinis foot, in order to reasonably develop Testudinis foot resource, be necessary to carry out artificial increasing cultivation's research to Testudinis foot.
Zhi Ming lotus section another belong to refer to tender tea leaves lotus belong to (
pollicipe) there is very large economic worth.In Spain, Portugal
p. polymeruswith
p. pollicipesbe regarded as delicious food especially, the fishery resources as a kind of huge economic value are highly developed utilization, have wide business demand and the very high market price, and the price of current per kilogram can up to 200 Euros.Due to excessive exploitation, for the needs of protection, the states such as Spain all limit its yield.
The life cycle of cirrus class usually will through battalion swim life 6 the phase nauplius, the cyprids of not ingesting, the young body of set and adult several stage of development.The cyprids of life of swimming grows young body stage of set, and its morphosis and life habit have a very large change.Cyprids is an important stage in cirrus class life cycle, and unique effect selects the substrate of suitable attachment and completes metamorphosis.Therefore, the cyprids settlement and metamorphosis that cirrus class is swum becomes the young body of set to be a key link in growth course; If cyprids can not complete settlement and metamorphosis, it finally only has death.Stockless cirrus class barnacle is topmost marine fouling organism, abroad with stricture of vagina barnacle (
balanus amphitrite) carried out studying for many years for the settlement and metamorphosis mechanism of model organism to barnacle.The ecological habit of Pedunculate Cirripedia (referring to that tender tea leaves lotus belongs to and Testudinis foot belongs to) and the different of stockless cirrus class barnacle, in the research that finger tender tea leaves lotus is belonged to, also attempt to induce the settlement and metamorphosis of cyprids, but all only obtain accidental extremely low distortion ratio, for the induction of the settlement and metamorphosis of its cyprids, the whole world is so far there are no successful report.
Although Testudinis foot cyprids can be gone out in indoor large-scale culture at present, the artificial breeding of Testudinis foot be realized, a difficult problem of inducing Testudinis foot cyprids settlement and metamorphosis under artificial condition must be broken through.The settlement and metamorphosis of Testudinis foot cyprids be complete its life cycle in indoor must through process, also be the key problem realizing Testudinis foot artificial breeding, therefore need to filter out the method or preparation that can induce Testudinis foot cyprids settlement and metamorphosis.
Summary of the invention
Do not have a kind of suitable method or preparation can induce the settlement and metamorphosis of Testudinis foot cyprids at present.According to the result of study in our early stage, the present invention is mixed with the preparation of Testudinis foot cyprids settlement and metamorphosis with juvenile hormone-III Main Function composition, then adds filtering sea and be mixed with induction sea water; This using method is simple, uses the distortion ratio of rear cyprids to reach more than 95%, and the young body of acquisition can be grown by normal growth.
In order to realize above-mentioned target, the technical scheme that the present invention takes is: the preparation of Testudinis foot cyprids settlement and metamorphosis, in water body, pharmaceutical formulation is composed as follows: be 1/25(w/v with dimethyl sulfoxide according to juvenile hormone-III/dimethyl sulfoxide) bulking value ratio hydrotropy, add ethanol and be mixed with preparation containing juvenile hormone-III 0.1mg/mL, preserve under-20 DEG C of conditions.Add filtering sea before using and be mixed with induction sea water.
Juvenile hormone-III is contained within the scope of 0.2 ~ 0.4 mg in every milliliter of induction sea water.
Described induction sea water service condition is: water temperature is 23 ~ 30 DEG C, and salinity is 25 ~ 35, pH 7.8 ~ 8.1;
Cultivate and collect cyprids, cyprids is introduced after soaking induction 6 ~ 12h in induction sea water, change fresh seawater, can settlement and metamorphosis be completed through 3-4d cyprids.
The present invention is simple to operate, with low cost, and effect is stable, and inducing effect is remarkable, and distortion ratio can reach 86%.Method presents the suitable concn of formulation application, processing time and induction starting time, be a kind of good method of inducing Testudinis foot cyprids settlement and metamorphosis, cyprids distortion ratio is all up to 95%.
Detailed description of the invention
The present invention induces for continuous 3 years in multiple batches of Testudinis foot cyprids, all reaches good effect.The present invention is described further for following examples mode.
Embodiment 1
Be 1/25(w/v with dimethyl sulfoxide according to juvenile hormone-III/dimethyl sulfoxide) ratio hydrotropy, add ethanol and be mixed with preparation containing juvenile hormone-III 0.1mg/mL, preserve under-20 DEG C of conditions.Add filtering sea before using to be mixed with containing juvenile hormone-III at 0.2 mg induction sea water.Cultivate and collect cyprids, introduced by cyprids in induction sea water after immersion treatment 8h, change fresh seawater, after 4d inducing culture, cyprids can complete settlement and metamorphosis, and distortion ratio reaches 92.4%.
Described induction sea water service condition is: water temperature is 25 DEG C, and salinity is 28, pH 7.8, and induction time is 8h.
Embodiment 2
Be 1/25(w/v with dimethyl sulfoxide according to juvenile hormone-III/dimethyl sulfoxide) ratio hydrotropy, add ethanol and be mixed with preparation containing juvenile hormone-III 0.1mg/mL, preserve under-20 DEG C of conditions.Add filtering sea before using to be mixed with containing juvenile hormone-III at 0.4 mg induction sea water 100mL.Cultivate and collect cyprids, introduced by 1000 cyprids in induction sea water after immersion treatment 12h, change fresh seawater, after 4d inducing culture, cyprids can complete settlement and metamorphosis, and distortion ratio reaches 95%.
Embodiment 3
Be 1/25(w/v with dimethyl sulfoxide according to juvenile hormone-III/dimethyl sulfoxide) ratio hydrotropy, add ethanol and be mixed with preparation containing juvenile hormone-III 0.1mg/mL, preserve under-20 DEG C of conditions.Add filtering sea before using to be mixed with containing juvenile hormone-III at 0.3 mg induction sea water 400mL.Cultivate and collect cyprids, introduced by 3200 cyprids in induction sea water after immersion treatment 6h, change fresh seawater, after 5d inducing culture, have 2870 larval metamorphosis altogether, distortion ratio reaches 89.6%.
The settlement and metamorphosis inducing effect of the pharmaceutical formulation and methods for using them that the present invention provides to Testudinis foot cyprids is good.
Claims (3)
1. the method utilizing juvenile hormone-III to induce Testudinis foot cyprids settlement and metamorphosis, it is characterized in that: be 1/25(w/v with dimethyl sulfoxide according to juvenile hormone-III/dimethyl sulfoxide) bulking value ratio hydrotropy, add ethanol and be mixed with preparation containing juvenile hormone-III 0.1mg/mL, preserve under-20 DEG C of conditions; Add filtering sea before using and be mixed with induction sea water;
Cultivate and collect cyprids, cyprids is introduced after inducing immersion 6 ~ 12h in induction sea water, change fresh seawater, can settlement and metamorphosis be completed through 3 ~ 4d cyprids.
2. a kind of method utilizing juvenile hormone-III to induce Testudinis foot cyprids settlement and metamorphosis according to claim 1, is characterized in that described induction sea water, contains juvenile hormone-III within the scope of 0.2 ~ 0.4 mg in every milliliter of induction sea water.
3. a kind of method utilizing juvenile hormone-III to induce Testudinis foot cyprids settlement and metamorphosis according to claim 1, it is characterized in that described induction sea water: water temperature is 23 ~ 30 DEG C, salinity is 25 ~ 35, pH 7.8 ~ 8.1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510183853.8A CN104784112B (en) | 2015-04-20 | 2015-04-20 | A kind of method that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone III |
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| CN201510183853.8A CN104784112B (en) | 2015-04-20 | 2015-04-20 | A kind of method that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone III |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326567A (en) * | 2019-08-01 | 2019-10-15 | 福建师范大学 | A kind of smooth medicine combined techniques induction tortoise young body artificial raise seedling method enough |
| CN110419475A (en) * | 2019-08-01 | 2019-11-08 | 福建师范大学 | A kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method |
| CN110495413A (en) * | 2019-08-01 | 2019-11-26 | 福建师范大学 | A method of induction tortoise foot cypris-form larva settlement and metamorphosis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578967A (en) * | 2009-06-16 | 2009-11-18 | 中国科学院南海海洋研究所 | Method for culturing cyprids of balanus reticulates on large scale |
| US8657943B2 (en) * | 2010-11-30 | 2014-02-25 | Ufrj, Ieapm, Uff | 1-hydroxy-2-O-acyl-sn-glycero-3-phosphocholine compounds, preparation process, antifouling composition, process for its preparation, method to prevent fouling, method to turn a surface into an antifouling surface, and, covered surface |
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2015
- 2015-04-20 CN CN201510183853.8A patent/CN104784112B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578967A (en) * | 2009-06-16 | 2009-11-18 | 中国科学院南海海洋研究所 | Method for culturing cyprids of balanus reticulates on large scale |
| US8657943B2 (en) * | 2010-11-30 | 2014-02-25 | Ufrj, Ieapm, Uff | 1-hydroxy-2-O-acyl-sn-glycero-3-phosphocholine compounds, preparation process, antifouling composition, process for its preparation, method to prevent fouling, method to turn a surface into an antifouling surface, and, covered surface |
Non-Patent Citations (1)
| Title |
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| EDGARDO D. GOMEZ,ET.AL: "Juvenile Hormone Mimics: Effect on Cirriped Crustacean Metamorphosis", 《SCIENCE》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326567A (en) * | 2019-08-01 | 2019-10-15 | 福建师范大学 | A kind of smooth medicine combined techniques induction tortoise young body artificial raise seedling method enough |
| CN110419475A (en) * | 2019-08-01 | 2019-11-08 | 福建师范大学 | A kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method |
| CN110495413A (en) * | 2019-08-01 | 2019-11-26 | 福建师范大学 | A method of induction tortoise foot cypris-form larva settlement and metamorphosis |
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| CN104784112B (en) | 2017-12-19 |
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