CN104784112B - A kind of method that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone III - Google Patents
A kind of method that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone III Download PDFInfo
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- CN104784112B CN104784112B CN201510183853.8A CN201510183853A CN104784112B CN 104784112 B CN104784112 B CN 104784112B CN 201510183853 A CN201510183853 A CN 201510183853A CN 104784112 B CN104784112 B CN 104784112B
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Abstract
The present invention relates to the technical field of aquaculture, and in particular to a kind of preparation and application process that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone III.The technical scheme that the present invention takes is：The preparation of tortoise foot cyprids settlement and metamorphosis, pharmaceutical formulation composition is as follows in water body：With dimethyl sulfoxide (DMSO) according to III/dimethyl sulfoxide (DMSO) of juvenile hormone be 1/25（w/v）Bulking value ratio hydrotropy, add ethanol to be configured to the preparation containing the 0.1mg/mL of juvenile hormone III, preserved under the conditions of 20 DEG C.It is configured to induce seawater using preceding addition filtering sea.The present invention is simple to operate, and cost is cheap, and effect is stable, and inducing effect is notable, and distortion ratio is up to 86%.Method presents the suitable concn of formulation application, processing time and induction starting time, is a kind of good method for inducing tortoise foot cyprids settlement and metamorphosis, and cyprids distortion ratio is up to 95%.
Description
Technical field
The present invention relates to the technical field of aquaculture, and in particular to one kind induces tortoise pure gold star using juvenile hormone-III
The preparation and application process of larva settlement and metamorphosis
Background technology
Tortoise foot（Capitulum mitellaLinnaeus）It is under the jurisdiction of Arthropoda（Arthropoda）Crust subphylum
（Crustacea）Cirrus infraclass（Cirripedia）Enclose chest catalogue（Thoracica）There is handle mesh（Pedunculata）Armour tender tea leaves lotus is sub-
Mesh（Scalpellomorpha）Zhi Ming lotuses section（Pollicipidae）Tortoise belongs to enough（Capitulum）, because its bodily form similar to tortoise pin and
Gain the name, also known as barnacle, Buddha's hand, tortoise pin, penholder etc..It is distributed mainly in the rock slot of Perenniporia martius marine site intertidal zone.
Tortoise has higher a nutritive value and medical value enough, and the tortoise on domestic market is enough fished by natural sea area, is had
Certain difficulty, and certain destruction is caused to the habitat of tortoise foot, in order to reasonably develop tortoise foot resource, it is necessary to right
Tortoise carries out artificial increasing cultivation's research enough.
Another category of Zhi Ming lotuses section refers to tender tea leaves lotus category（Pollicipe）With very big economic value.In Spain, PortugalP. polymerusWithP. pollicipesDelicious food is more regarded as, as a kind of fishery resources of huge economic value
Utilization is highly developed, there is wide business demand and the very high market price, the price of per kilogram may be up to 200 Europe at present
Member.Due to excessive utilization, its yield is limited for states such as the needs of protection, Spain.
The history of life of cirrus class is typically passed through battalion and swum 6 phase nauplius of life, the cyprids do not ingested, set
Young body and adult several stages of development.Swim life cyprids development to set the young body stage, its morphosis and
Life habit has a very large change.Cyprids are an important stages in the cirrus class history of life, and unique effect is
Select the matrix suitably adhered to and complete metamorphosis.Therefore, the cyprids settlement and metamorphosis that cirrus class is swum is into the young body of set
A key link in growth course；If cyprids can not complete settlement and metamorphosis, its final only death.Stockless cirrus
Class barnacle is most important marine fouling organism, it is external with line barnacle (Balanus amphitrite) for model organism to rattan
The settlement and metamorphosis mechanism of kettle is studied for many years.Pedunculate Cirripedia（Refer to tender tea leaves lotus category and tortoise belongs to enough）Ecological habit and stockless it is climing
Sufficient class barnacle it is different, also attempt to induce the settlement and metamorphosis of cyprids in the research to referring to tender tea leaves lotus category, but all only obtain
Accidental extremely low distortion ratio, for the induction of the settlement and metamorphosis of its cyprids, the whole world are obtained so far there are no successfully reporting.
Although at present large-scale culture tortoise foot cyprids can be gone out indoors, the artificial breeding of tortoise foot is realized, must
The problem that tortoise foot cyprids settlement and metamorphosis is induced under artificial condition must be broken through.The settlement and metamorphosis of tortoise foot cyprids is in room
It is interior complete its history of life must be through process, and realize the key problem of tortoise foot artificial breeding, it is therefore desirable to which filtering out to lure
Lead the method or preparation of tortoise foot cyprids settlement and metamorphosis.
The content of the invention
The settlement and metamorphosis of tortoise foot cyprids can be induced currently without a kind of suitable method or preparation.According to before us
The result of study of phase, the present invention are configured to the preparation of tortoise foot cyprids settlement and metamorphosis with the main function composition of juvenile hormone-III；
Said preparation application method is simple, can reach more than 95% using the distortion ratio of rear cyprids, the young body of acquisition being capable of normal growth
Development.
In order to realize above-mentioned target, the technical scheme that the present invention takes is：The preparation of tortoise foot cyprids settlement and metamorphosis,
Pharmaceutical formulation composition is as follows in water body：With dimethyl sulfoxide (DMSO) according to juvenile hormone-III/dimethyl sulfoxide (DMSO) be 1/25（w/v）Weight
Volume ratio hydrotropy is measured, adds ethanol to be configured to the preparation containing the 0.1mg/mL of juvenile hormone-III, is preserved under the conditions of -20 DEG C.Make
It is configured to induce seawater with preceding addition filtering sea.
Described induction seawater use condition is：Water temperature is 23 ~ 30 DEG C, and salinity is 25 ~ 35, pH 7.8 ~ 8.1.
Cyprids are collected in culture, and cyprids are introduced into induction seawater after 6~12h of immersion induction, change fresh sea
Water, settlement and metamorphosis can be completed through 3-4d cyprids.
The present invention is simple to operate, and cost is cheap, and effect is stable, and inducing effect is notable, and distortion ratio is up to 86%.This method is given
Suitable concn, processing time and the induction starting time of formulation application are gone out, have been a kind of the good of induction tortoise foot cyprids settlement and metamorphosis
Method, cyprids distortion ratio are up to 95%.
Embodiment
The present invention is induced for continuous 3 years in multiple batches of tortoise foot cyprids, reaches good effect.Below
The present invention is described further for way of example.
Embodiment 1
With dimethyl sulfoxide (DMSO) according to juvenile hormone-III/dimethyl sulfoxide (DMSO) be 1/25（w/v）Ratio hydrotropy, add ethanol to match somebody with somebody
The preparation containing the 0.1mg/mL of juvenile hormone-III is made, is preserved under the conditions of -20 DEG C.It is configured to often using preceding addition filtering sea
Rise and induce seawater in 0.2 mg containing juvenile hormone-III.Cyprids are collected in culture, and cyprids are introduced into induction seawater and soaked
After bubble processing 8h, fresh seawater is changed, cyprids can complete settlement and metamorphosis after 4d Fiber differentiations, and distortion ratio reaches 92.4%.
Described induction seawater use condition is：Water temperature is 25 DEG C, salinity 28, pH 7.8, induction time 8h.
Embodiment 2
With dimethyl sulfoxide (DMSO) according to juvenile hormone-III/dimethyl sulfoxide (DMSO) be 1/25（w/v）Ratio hydrotropy, add ethanol to match somebody with somebody
The preparation containing the 0.1mg/mL of juvenile hormone-III is made, is preserved under the conditions of -20 DEG C.It is configured to often using preceding addition filtering sea
Rise containing juvenile hormone-III in 0.4 mg induction seawater 100mL.Cyprids are collected in culture, and 1000 cyprids are introduced
Induce in seawater after immersion treatment 12h, change fresh seawater, cyprids can complete settlement and metamorphosis after 4d Fiber differentiations, become
State rate reaches 95%.
Embodiment 3
With dimethyl sulfoxide (DMSO) according to juvenile hormone-III/dimethyl sulfoxide (DMSO) be 1/25（w/v）Ratio hydrotropy, add ethanol to match somebody with somebody
The preparation containing the 0.1mg/mL of juvenile hormone-III is made, is preserved under the conditions of -20 DEG C.It is configured to often using preceding addition filtering sea
Rise containing juvenile hormone-III in 0.3 mg induction seawater 400mL.Cyprids are collected in culture, and 3200 cyprids are introduced
Induce in seawater after immersion treatment 6h, change fresh seawater, after 5d Fiber differentiations, there are 2870 larval metamorphosis altogether, it is abnormal
Rate is up to 89.6%.
The pharmaceutical formulation and methods for using them that the present invention provides is good to the settlement and metamorphosis inducing effect of tortoise foot cyprids.
Claims (2)
- A kind of 1. method that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone-III, it is characterised in that：It is sub- with dimethyl Sulfone is 1/25 according to juvenile hormone-III/dimethyl sulfoxide (DMSO)（w/v）Bulking value ratio hydrotropy, add ethanol be configured to containing protect The young 0.1mg/mL of hormone-III preparation, preserve under the conditions of -20 DEG C；It is configured to induce seawater using preceding addition filtering sea；Cyprids are collected in culture, and cyprids are introduced into induction seawater after 6~12h of induction immersion, change fresh seawater, warp 3~4d cyprids can complete settlement and metamorphosis.
- 2. a kind of method that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone-III according to claim 1, its It is characterised by described induction seawater：Water temperature is 23 ~ 30 DEG C, and salinity is 25 ~ 35, pH 7.8 ~ 8.1.
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| CN201510183853.8A CN104784112B (en) | 2015-04-20 | 2015-04-20 | A kind of method that tortoise foot cyprids settlement and metamorphosis is induced using juvenile hormone III |
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| CN110419475A (en) * | 2019-08-01 | 2019-11-08 | 福建师范大学 | A kind of kinoprene dripping method step flow-induction tortoise pedal gland Jie's larval metamorphosis method |
| CN110326567B (en) * | 2019-08-01 | 2023-05-26 | 福建师范大学 | Method for inducing artificial seedling raising of turtle foot young body by photo-drug combination method |
| CN110495413A (en) * | 2019-08-01 | 2019-11-26 | 福建师范大学 | A method of induction tortoise foot cypris-form larva settlement and metamorphosis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578967A (en) * | 2009-06-16 | 2009-11-18 | 中国科学院南海海洋研究所 | Method for culturing cyprids of balanus reticulates on large scale |
| US8657943B2 (en) * | 2010-11-30 | 2014-02-25 | Ufrj, Ieapm, Uff | 1-hydroxy-2-O-acyl-sn-glycero-3-phosphocholine compounds, preparation process, antifouling composition, process for its preparation, method to prevent fouling, method to turn a surface into an antifouling surface, and, covered surface |
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2015
- 2015-04-20 CN CN201510183853.8A patent/CN104784112B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578967A (en) * | 2009-06-16 | 2009-11-18 | 中国科学院南海海洋研究所 | Method for culturing cyprids of balanus reticulates on large scale |
| US8657943B2 (en) * | 2010-11-30 | 2014-02-25 | Ufrj, Ieapm, Uff | 1-hydroxy-2-O-acyl-sn-glycero-3-phosphocholine compounds, preparation process, antifouling composition, process for its preparation, method to prevent fouling, method to turn a surface into an antifouling surface, and, covered surface |
Non-Patent Citations (1)
| Title |
|---|
| Juvenile Hormone Mimics: Effect on Cirriped Crustacean Metamorphosis;Edgardo D. Gomez,et.al;《Science》;19730223;第179卷(第4075期);第813-814页，尤其是摘要；第813页右栏第1段、第3段、表1；第814页左栏第3段 * |
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