CN102674563B - Novel environment-friendly efficient microorganism water quality purifying mixture - Google Patents
Novel environment-friendly efficient microorganism water quality purifying mixture Download PDFInfo
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- Farming Of Fish And Shellfish (AREA)
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Abstract
The invention discloses a novel environment-friendly efficient microorganism water quality purifying mixture and a preparation method thereof. The microorganism water quality purifying mixture is a mixed microorganism bacterial agent mainly consisting of high-concentration bacillus subtilis liquid and high-concentration bdellovibro bacteriovorus liquid, and is prepared from the following raw materials in percentage by volume: 10-50% of bacillus subtilis liquid, 20-60% of bdellovibro bacteriovorus liquid, 0.5-6.0% of normal saline and 0.5-15% of trace element solution. The novel environment-friendly efficient microorganism water quality purifying mixture disclosed by the invention integrates the advantages of the bacillus subtilis in purifying water quality and the bdellovibro bacteriovorus in preventing and treating diseases, has the functions of purifying water quality, preventing and treating diseases and replacing antibiotics, and realizes a function of improving the water for aquaculture.
Description
Technical field
The present invention relates to a kind of novel environment friendly microorganism purification of water quality mixture and preparation method thereof, can be used for aquaculture, purify water, the aspect such as environmental protection, belong to aquaculture system improving technology field.
Background technology
Since 20th century, China's culture fishery has obtained tremendous development, but along with the continuous expansion of aquaculture scale, the improving constantly of intensive culture degree, and the Eutrophic Extent of aquaculture pond is more and more higher.Because can not having met, aquaculture water self-cleaning and regulating power remove residual bait, the excremental needs of fishes and shrimps, cause the severe overweights such as ammonia nitrogen in aquaculture water, nitrate, nitrite, sulfide, water quality deterioration, fish and shrimp diseases occurs again and again, and 10,000,000,000 yuan of above serious financial losses have been caused to culture fishery every year in the whole nation.Therefore the objectionable impurities of how efficiently removing in aquaculture water is the key that guarantees China's culture fishery Sustainable development with the disease of preventing and treating aquatic animal.
Utilize efficient microbial strain to improve and recover the good ecotope of aquaculture water, by increasing people, accepted and put into practical application.Subtilis is as a kind of prebiotic bacterial classification safely and effectively, owing to producing many extracellular enzymes, decompose rapidly organism in water body, promote the oxidation of sulfide and nitrite, the ability that has Fast Growth breeding and conform, is therefore widely used in aquaculture industry.
Along with the continuous expansion of aquaculture scale and cultivation density improve constantly, China's aquaculture system self-cleaning and regulating power can not meet removes residual bait and the excremental needs of fishes and shrimps, cause the severe overweights such as ammonia nitrogen in aquaculture water, nitrate, nitrite, sulfide, water quality deterioration, fish and shrimp diseases occurs again and again.Simultaneously antibiotic a large amount of frequent use brings very large impact also to quality and the outlet of aquatic animal.At present, single prebiotic bacterial classification is applied in the generation that still can not effectively improve Water quality and fish and shrimp diseases in aquaculture industry.
Summary of the invention
In order to solve the problem of prior art, the object of the present invention is to provide a kind of novel environment friendly microorganism purification of water quality mixture, its subtilis is purified water and the advantage set of having a liking for bacterium Bdellovibrio disease preventing and treating in one, possess simultaneously and purify water and prevent and cure diseases and replace antibiotic function, can improve aquaculture system.
Another object of the present invention is to provide a kind of preparation method of above-mentioned novel environment friendly microorganism purification of water quality mixture.
For achieving the above object, technical scheme of the present invention provides a kind of novel environment friendly microorganism purification of water quality mixture, the mixing microorganisms microbial inoculum mainly being formed by high density subtilis liquid and high density bacteriophagic Bdellovibrio liquid, this microorganism purification of water quality mixture is formed by the preparation of raw material of following volume percent:
Furthermore, each raw material optimal volume proportioning of the present invention is:
The bacterial classification of novel environment friendly microorganism purification of water quality mixture of the present invention is subtilis and bacteriophagic Bdellovibrio mixture; Described subtilis liquid is bacillus, and its concentration is for being greater than 20,000,000,000/ml; Described bacteriophagic Bdellovibrio liquid, its concentration is for being greater than 20,000,000,000/ml.
The mixing solutions that described trace element solution comprises copper sulfate, zinc sulfate, manganous sulfate, calcium chloride.
In addition, the present invention also provides a kind of making method of novel environment friendly microorganism purification of water quality mixture, and all bacterial classifications of employing are all to filter out from the water body of aquaculture and bed mud; Adopt bacterial classification liquid cultivating method, all bacterial classifications are cultivated in different culture media; So latter two bacterium liquid is mixed in proportion again; It comprises following rapid:
(1) from the water body of aquaculture and bed mud, filter out subtilis and bacteriophagic Bdellovibrio bacterial classification;
(2) Bacillus subtilis strain is cultivated in medium component is the mixture of yeast extract paste, molasses, peptone, sodium-chlor, ferric sulfate, magnesium sulfate, trace element solution, pH value is adjusted to 6.8-7.2; Culture temperature is that 35-38 ℃, time are 16-48 hour, obtains subtilis liquid;
(3) intestinal bacteria bacterial classification is cultivated in medium component is the mixture of yeast extract paste, molasses, peptone, sodium-chlor, ferric sulfate, peace magnesium sulfate, trace element solution, pH value is adjusted to 6.8-7.2; Culture temperature is that 35-38 ℃, time are 16-48 hour, obtains intestinal bacteria liquid; Then add bacteriophagic Bdellovibrio bacterial classification, pH value is adjusted to 6.8-7.2; Culture temperature is 30-35 ℃, continues to cultivate 36-48 hour, obtains bacteriophagic Bdellovibrio liquid.
(4) the high density subtilis liquid making and high density bacteriophagic Bdellovibrio liquid are added to physiological saline, liquid microelement uniformly mixes, and obtains novel environment friendly microorganism purification of water quality mixture.
By technique scheme, advantage and beneficial effect that the present invention has are as follows:
1) bacteriophagic Bdellovibrio can parasitize in other bacterium, and in unwanted bacteria, continues breeding, can make the death of breaking of harmful bacteria in water body, thereby reach prophylactic effect;
2) subtilis can be decomposed residual bait in aquaculture water and the organic pollutants such as movement of hydrocoles, in and nitrite, the toxicity of the material such as manganese, copper, mercury in degradation water, control the formation that is harmful to water body and the growth and breeding that promotes useful plankton in muddy muddy water body, thus the benign state that water conditioning is grown up healthy and sound at applicable aquaculture thing;
3) increase the food consumption of aquaculture thing, significantly improve surviving rate and efficiency of feed utilization, thereby reduce feeding cost, improve cultivation efficiency;
4) effectively prevent aquatic products material culture disease to occur, strengthen aquaculture thing pipe intestinal digesting absorptive function;
5) can remove peculiar smell and have the effect of oxygenation, to fish and shrimp without bad stress reaction;
6) effectively control the bacteriosis of aquaculture thing and the generations of virus such as the rotted gill disease of light, seawater fish and shrimp, crab, soft-shelled turtle, tortoise, the frog, shellfish and spread, crab is trembled to disease, gonorrhoea are sick, peach is drawn disease etc. have obvious curative effects;
7) guarantee aquaculture safety, efficient, have no side effect, noresidue, pollution-free.
Embodiment
By specific embodiment and effect experimental example, respectively novel environment friendly microorganism purification of water quality mixture of the present invention and preparation method thereof is expressed in further detail below, but the present invention is not limited in following embodiment.
Novel environment friendly microorganism purification of water quality mixture of the present invention, it is the mixed bacteria liquid that the water body from aquaculture becomes with bacteriophagic Bdellovibrio bacterial classification spawn culture with the subtilis filtering out bed mud, it is rich in monomeric protein, amino acid, organized enzyme, polyphenoils, VITAMIN and other various physiologically active substances, there is oxidation, ammonification, nitrated, phosphorus decomposing, sulfuration and solid ammonia effect, objectionable impurities can be decomposed into sulfurous gas, nitrate etc., not only purify water, and can provide nutrition for the breeding of unicellular algae.And can be according to: subtilis liquid 10%-50%; In bacteriophagic Bdellovibrio liquid 20%-60%, both arbitrary ratios mixing can make purification of water quality mixture.
embodiment 1
the preparation of novel environment friendly microorganism purification of water quality mixture of the present invention
A, take yeast extract paste 10g, molasses 15g, peptone 10g, sodium-chlor 5g, ferric sulfate 0.5g, magnesium sulfate 1g, trace element solution 5ml, water 500ml.
Above raw material is added in 1000ml fermentor tank, and pH value is adjusted to 6.8-7.2, is heated to 121 ℃ of sterilizings 30 minutes, adds Bacillus subtilis strain while being cooled to 37 ℃, and culture temperature is that 36-38 ℃, time are 32 hours, obtains subtilis liquid.
B, take yeast extract paste 10g, molasses 15g, peptone 10g, sodium-chlor 5g, ferric sulfate 0.5g, magnesium sulfate 1g, trace element solution 5ml, water 500ml.
Above raw material is added in 1000ml fermentor tank, and pH value is adjusted to 6.8-7.2, is heated to 121 ℃ of sterilizings 30 minutes, while being cooled to 37 ℃, add enterobacteria bacterial classification, culture temperature is that 36-38 ℃, time are 24 hours, then adds bacteriophagic Bdellovibrio bacterial classification, and pH value is adjusted to 6.8-7.2; Culture temperature is 30-35 ℃, continues to cultivate 36 hours, obtains bacteriophagic Bdellovibrio liquid.
By following optimal components ratio, take:
Novel environment friendly microorganism purification of water quality mixture stirs to obtain.
embodiment 2
the preparation of novel environment friendly microorganism purification of water quality mixture of the present invention
A, take yeast extract paste 10g, molasses 15g, peptone 10g, sodium-chlor 5g, ferric sulfate 0.5g, magnesium sulfate 1g, trace element solution 5ml, water 500ml.
Above raw material is added in 1000ml fermentor tank, and pH value is adjusted to 6.8-7.2, is heated to the sterilizing 30 minutes of 121 ℃ of degree, adds Bacillus subtilis strain while being cooled to 37 ℃, and culture temperature is that 36-38 ℃, time are 32 hours, obtains subtilis liquid.
B, take yeast extract paste 10g, molasses 15g, peptone 10g, sodium-chlor 5g, ferric sulfate 0.5g, magnesium sulfate 1g, trace element solution 5ml, water 500ml.
Above raw material is added in 1000ml fermentor tank, and pH value is adjusted to 6.8-7.2, is heated to 121 ℃ of degree sterilizing 30 minutes, be cooled to 37 ℃ and add enterobacteria bacterial classification while spending, culture temperature is that 36-38 ℃, time are 24 hours, then adds bacteriophagic Bdellovibrio bacterial classification, and pH value is adjusted to 6.8-7.2; Culture temperature is 30-35 ℃, continues to cultivate 36 hours, obtains bacteriophagic Bdellovibrio liquid.
By following optimal components ratio, take:
Novel environment friendly microorganism purification of water quality mixture stirs to obtain.
effect experimental example 1
security of the present invention
In three fishponds, carry out safety testing, grass carp 50 tails are put in each fishpond in a suitable place to breed.In first fishpond, add 5ml novel environment friendly microorganism of the present invention purification of water quality mixture every day; In second fishpond, add 10ml novel environment friendly microorganism of the present invention purification of water quality mixture every day; In the 3rd fishpond, add 20ml novel environment friendly microorganism of the present invention purification of water quality mixture every day.Observe 30 days.
In 30 days, the fish in three fishponds is all without abnormal, the depressed or phenomena of mortality that are a cup too low, well-grown.Illustrate that novel environment friendly microorganism purification of water quality mixture safety performance of the present invention is good.
effect experimental example 2
the prophylactic effect of the present invention to aquaculture bacteriosis
In four fishponds, carry out the test of bacterial disease prophylactic effect.Grass carp 30 tails are put in each fishpond, and every urosome is 100～150g heavily approximately.Normally feed every day in first fishpond; Throw something and feed with 2 grams of spices of Chinese medicine every day in second fishpond; The 3rd fishpond adds 1 gram of spice of 5% Enrofloxacin soluble powder to throw something and feed; The 4th pond adds novel environment friendly microorganism purification of water quality mixture 2ml of the present invention;
Raise after one week, in four fishponds, respectively put into respectively a grass carp that suffers from vibrio infection, observe the growing state of fish in each fishpond.The results are shown in Table 1.
The prophylactic effect of table 1 novel environment friendly microorganism of the present invention purification of water quality mixture to grass carp bacteriosis
Table 1 result shows that novel environment friendly microorganism purification of water quality mixture of the present invention has obvious preventive and therapeutic action to grass carp bacteriosis.
effect experimental example 3
the therapeutic action of the present invention to aquaculture bacteriosis
200, the grass carp with suffering from bacterial infection disease, is divided into four groups at random, and 50 every group, normally feed first group of every day; Second group adds 4 grams of Chinese medicine powder, and spice is thrown something and fed; The 3rd group with 2 grams of 5% Enrofloxacin soluble powders, and spice is thrown something and fed; The 4th group with 4 grams of novel environment friendly microorganism purification of water quality mixtures of the present invention;
Raise after one week, observe the growing state of all grass carps.The results are shown in Table 2.
The therapeutic action of table 2 novel environment friendly microorganism of the present invention purification of water quality mixture to grass carp bacteriosis
Table 2 result shows, mix bacterium agent of the present invention has obvious therapeutic action to grass carp bacteriosis.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any pro forma restriction, therefore every, do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the modification above embodiment done according to technical spirit of the present invention, all still belong in the scope of technical solution of the present invention.
Claims (5)
1. a novel environment friendly microorganism purification of water quality mixture, is characterized in that: it is mainly formulated by subtilis liquid and bacteriophagic Bdellovibrio liquid; This purification of water quality mixture is formed by each preparation of raw material of following volume percent:
The mixing solutions that described trace element solution comprises copper sulfate, zinc sulfate, manganous sulfate and calcium chloride;
The preparation method of described novel environment friendly microorganism purification of water quality mixture, adopts bacterial classification liquid cultivating method, and all bacterial classifications are cultivated in different culture media; So latter two bacterium liquid is mixed in proportion again; This making method comprises the following steps:
(1) from the water body of aquaculture and bed mud, filter out subtilis and high density bacteriophagic Bdellovibrio bacterial classification;
(2) Bacillus subtilis strain is cultivated in medium component is the mixture of yeast extract paste, molasses, peptone, sodium-chlor, ferric sulfate, magnesium sulfate and trace element solution, obtained subtilis liquid;
(3) intestinal bacteria bacterial classification is cultivated in medium component is the mixture of yeast extract paste, molasses, peptone, sodium-chlor, ferric sulfate, magnesium sulfate and trace element solution, obtained intestinal bacteria liquid; Then add bacteriophagic Bdellovibrio bacterial classification to continue to cultivate, obtain bacteriophagic Bdellovibrio liquid;
(4) the high density subtilis liquid making and high density bacteriophagic Bdellovibrio liquid are added to physiological saline, trace element solution uniformly mixes, and obtains novel environment friendly microorganism purification of water quality mixture.
2. microorganism purification of water quality mixture according to claim 1, is characterized in that: each preparation of raw material by following volume percent forms:
3. microorganism purification of water quality mixture according to claim 1 and 2, is characterized in that: in step (2), the pH value of cultivation is adjusted to 6.8-7.2; Culture temperature is that 35-38 ℃, time are 16-48 hour.
4. microorganism purification of water quality mixture according to claim 1 and 2, is characterized in that: in step (3), the pH value of cultivating intestinal bacteria liquid is adjusted to 6.8-7.2, and culture temperature is 35-38 ℃, and the time is 16-48 hour; The pH value of cultivating bacteriophagic Bdellovibrio liquid is adjusted to 6.8-7.2, and culture temperature is 30-35 ℃, and the time is 36-48 hour.
5. microorganism purification of water quality mixture according to claim 1 and 2, is characterized in that: in step (4), its concentration of described physiological saline is 0.9%; The mixing solutions that described trace element solution comprises copper sulfate, zinc sulfate, manganous sulfate and calcium chloride, its concentration is 0.5%.
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| CN103663726B (en) * | 2013-12-09 | 2015-04-08 | 山东华亚环保科技有限公司 | Preparation method of microbiological water purifier |
| CN103740613B (en) * | 2013-12-20 | 2016-01-13 | 毕景阳 | A kind of preparation method of biological active bacterium agent |
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| CN104099268A (en) * | 2014-06-17 | 2014-10-15 | 贝嘉美（天津）生物技术研发有限公司 | Aquaculture composite microbial agent |
| CN105309342B (en) * | 2014-07-29 | 2018-05-01 | 上海海洋大学 | A kind of method for lifting Penaeus Vannmei nursery live seedling amount and cultured output |
| CN104472548B (en) * | 2014-11-25 | 2017-12-05 | 上海创博生态工程有限公司 | A kind of microorganism plant growth accelerator and its preparation method and application |
| CN104431521A (en) * | 2014-12-10 | 2015-03-25 | 苏州埃瑞特生物技术有限公司 | Preparation method of powdery preparation of bacillus pumilus and bdellovibrio |
| CN106854628A (en) * | 2015-12-08 | 2017-06-16 | 上海泓宝绿色水产股份有限公司 | A kind of micro- raw bacterium of composite disinfecting and its preparation and application |
| CN107473395A (en) * | 2017-07-25 | 2017-12-15 | 明光市永言水产(集团)有限公司 | A kind of water quality modifying agent |
| CN110169982A (en) * | 2019-06-06 | 2019-08-27 | 厦门惠盈动物科技有限公司 | A kind of phage bdellovibro preparation and its application for preventing batrachia torticollis disease |
| CN114591859A (en) * | 2022-03-18 | 2022-06-07 | 山东合创环保科技有限公司 | Microbial agent formula for water quality improvement and preparation method thereof |
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