CN109133368A - A kind of complex microorganism water purification agent and preparation method thereof for aquaculture - Google Patents
A kind of complex microorganism water purification agent and preparation method thereof for aquaculture Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
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Abstract
The complex microorganism water purification agent and preparation method thereof that the invention discloses a kind of for aquaculture, according to parts by weight, the complex microorganism water purification agent includes bacillus subtilis 18-24 parts by weight, clostridium butyricum 25-45 parts by weight, bacillus cereus 20-30 parts by weight, saccharomycete 14-20 parts by weight, green alga 12-18 parts by weight, zeolite 90-100 parts by weight and auxiliary material 10-20 parts by weight.Water purification agent of the invention is compound using microorganism and algae, pass through the synergistic effect of each mushroom, effectively remove the harmful substance in aquaculture waters, the treatment effeciency of nitrite is significantly improved after addition clostridium butyricum, and in water purification agent microorganism mass propagation and metabolite, the quickly and effectively organic matter in degradation water body, decomposes the multiple harmful substances such as ammoniacal nitrogen, nitrite, sulfide and phosphorus.
Description
Technical field
The invention belongs to field of water quality purification, and in particular to a kind of complex microorganism water purification agent for aquaculture and its
Preparation method.
Background technique
Penaeus Vannmei source area grows the climate zone torrid zone, subtropical zone, warm temperate zone, temperature in South America Pacific coast waters
Band sea area is distributed in the middle part of Pacific Ocean West Coast to the Gulf of Mexico and is mainly distributed northern Peru to Gulf of Mexico bank, with strategic point
The distribution of melon Dorr bank is concentrated the most, and life cycle 1 year.The thin body fertilizer of Penaeus Vannmei shell, delicious meat, dressed fish is high, nutrition
It is abundant.Penaeus Vannmei has many advantages, such as that individual is big, growth is fast, nutritional need is low, premunition is strong, to water environment factors
The advantages that adaptability is stronger, low to forage protein content requirement, and dressing percentage is up to 65% or more, out-of-water survival time is long be
The highest shrimp species of single rate in the excellent variety of intensive high yielding culture, and current three big cultured prawns in the world.
Now with the continuous development of aquaculture, Penaeus Vannmei high-density breeding scale constantly expands, and has accumulated big
The feed residual bait of amount and the excreta of aquatic livestock, pollute organic matter and increase, Water-environment Ecological System disequilibrium, water body
In nitrite severely exceed, cause the large area of aquatic animal disease to break out, affect the healthy growth of aquatic livestock, no
Only there is direct damaging effect to cultivated animals, while the water environment deteriorated makes pathogenic bacteria mass propagation, leads to outbreak of disease.Often
The settling mode of rule is that certain interval of time carries out change water to process, but changes water and on the one hand improve production and management cost, another
What aspect also easily caused shrimps is not suitable with symptom.
Microorganism can repair water body, have the function of nontoxic, without side-effects, noresidue, safe and reliable, microorganism
Preparation has been widely recognized for reducing the ammonia nitrogen and nitrite of water body, has already appeared the microorganism much compounded at present
Preparation, however using effect is unstable, the nitrobacteria of autotrophic type is relative to nitrite bacteria, and reproduction speed is slow, and not
Suitable for the breeding water body of water body serious eutrophication, especially after water quality deterioration, pond dissolved oxygen is in reduced levels for a long time,
The nitrobacteria of investment is limited to the degradation of ammonia nitrogen and nitrite, therefore usually occurs that using effect is unstable to ask
Topic, existing strain regulating water quality spreads unchecked, and each bacteria containing amount height is different, causes raiser's blindness medication, not only without effect,
Also create the raising of aquaculture cost.
Based on the above reasons, the present invention is specifically proposed.
Summary of the invention
In order to solve problem above of the existing technology, the present invention provides a kind of composite microbials for aquaculture
Object water purification agent and preparation method thereof, water purification agent of the invention are mainly the quality problem improved in culture of Penaeus vannamei waters,
It can be not only substantially reduced the content of nitrite, but also bacteriosis can be reduced, water purification agent of the invention passes through each
The synergistic effect of a strain and auxiliary material, increases the quantity containing viable bacteria of complex micro organism fungicide, while playing physical cleaning
With the effect of chemical cleaning method, purification is quickly thorough, is suitable for large-scale production and promotes.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of complex microorganism water purification agent for aquaculture, according to parts by weight, the complex microorganism water purification agent
Including bacillus subtilis 18-24 parts by weight, clostridium butyricum 25-45 parts by weight, bacillus cereus 20-30 parts by weight, yeast
Bacterium 14-20 parts by weight, green alga 12-18 parts by weight, zeolite 90-100 parts by weight and auxiliary material 10-20 parts by weight.
Bacillus subtilis can reduce water body ammonia nitrogen and nitrite level, and bacillus cereus is using carbon source as electricity
Sub- donor, nitrate anion and nitrite anions are reduced into nitrogen as electron acceptor, by NO3-N and NO2-N, while can also remove
The effect of organic matter and nitrogen pollutant；
The applicant has found through overtesting, can significantly improve the treatment effeciency of nitrite after clostridium butyricum is added, this
The mass propagation and metabolite of microorganism in the water purification agent of invention, quickly and effectively the organic matter in degradation water body, decomposes ammonia
The multiple harmful substances such as state nitrogen, nitrite, sulfide and phosphorus are eliminated or significantly by microbial reproduction and its metabolite
Water-bed siltation is reduced, purification improvement water quality safeguards the water body environment ecological balance, can effectively inhibit pathogen, hence it is evident that improves economical
Benefit；The purpose that green alga is also added into water purification agent of the invention is the nitrogen that can be gone in water removal by the breeding of green alga, phosphorus battalion
Support substance and heavy metal, moreover it is possible to form complicated cogeneration system with mushroom, promote the purification of water quality, green alga of the invention and mushroom
Syntrophism will not generate algae reproduction too fast the phenomenon that destroying the ecological balance.
Further, according to parts by weight, the complex microorganism water purification agent includes bacillus subtilis 20-22 weight
Part, clostridium butyricum 34-38 parts by weight, bacillus cereus 24-28 parts by weight, saccharomycete 16-18 parts by weight, green alga 14-16 weight
Measure part, zeolite 94-96 parts by weight and auxiliary material 14-16 parts by weight.
Further, the auxiliary material is sodium acetate and cornstarch.
Sodium acetate in auxiliary material promotes bacillus cereus to the degradation rate of ammonia nitrogen, nitrous acid Asia as carbon source；Corn
Starch can also be used as the replenishers of carbon source or small molecule carbon source, be also used as dispersing agent, the effect for keeping microbial inoculum dry.
Further, the mass ratio of sodium acetate and cornstarch is 1:0.8-1.2.
The present invention also provides the preparation method of the complex microorganism water purification agent for aquaculture, the sides
Method includes the following steps:
(1) it is weighed respectively according to the weight of each raw material spare；
(2) spare bacillus subtilis, clostridium butyricum, bacillus cereus and saccharomycete are mixed, is added beautiful
Rice starch and water, stir evenly, and carry out fermented and cultured and obtain microbial fermentation solution, spare；
(3) spare zeolite is crushed, is ground up, sieved processing；
(4) microbial fermentation solution is added to the water dilution, the zeolite, spare of step (3) processing is then added
Auxiliary material and green alga, uniformly mix, obtain the complex microorganism water purification agent for aquaculture.
Further, the temperature fermented in step (2) is 30-35 DEG C, and fermentation time is 3-4 days.
Further, the temperature fermented in step (2) is 32 DEG C, and fermentation time is 3.5 days.
The microbial fermentation solution prepared after fermentation process improves the activity of microorganism, increases complex microorganism
In contain number of viable, be greater than 30,000,000,000/g through the total viable count in detection microbial fermentation solution, while enhancing the ring of flora
Border adaptability enables continued propagation in water and purification.
Further, in step (2) bacillus subtilis, clostridium butyricum, bacillus cereus and saccharomycete gross mass
Mass ratio with water is 1:3-4.
Further, the total viable count in step (2) in microbial fermentation solution is greater than 30,000,000,000/g.
Further, the processing of 60-90 mesh is crossed in step (3).
The effect of adsorption treatment impurities in water and absorption microorganism that zeolite primarily serves, due to the physical property packet of zeolite
Density, bulk density, fineness, specific surface area etc. are included, zeolite of different nature will will affect the ability of water purification, and the present inventor passes through
Test discovery, treatment effect is preferable under 60-90 mesh, and the microorganism long period can be made to be suspended in water body.
Further, the volume ratio of microbial fermentation solution and water is 1:7-8 in step (4).
Compared with prior art, the invention has the benefit that
(1) water purification agent of the invention, by the synergistic effect of each mushroom, is effectively removed using the compound of microorganism and algae
Harmful substance in aquaculture waters significantly improves the treatment effeciency of nitrite, and water purification after clostridium butyricum is added
The mass propagation and metabolite of microorganism in agent, quickly and effectively the organic matter in degradation water body, decomposes ammoniacal nitrogen, nitrous acid
Water-bed silt is eliminated or greatly reduced to the multiple harmful substances such as salt, sulfide and phosphorus by microbial reproduction and its metabolite
Product, purification improvement water quality, safeguards the water body environment ecological balance, can effectively inhibit pathogen, hence it is evident that increase economic efficiency；
(2) water purification agent of the invention is nontoxic, without side-effects, does not generate drug resistance, can also inhibit pathogen growth, reduce and
Prevent the disease of aquatic livestock, maintain ecosystem balance, improves aquaculture ecological environment；
(3) preparation method of water purification agent of the invention is simple, and production cost is low, is easy to be mass produced.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
Embodiment 1
The complex microorganism water purification agent for aquaculture of the present embodiment, including bacillus subtilis 18kg, butyric acid shuttle
Bacterium 25kg, bacillus cereus 20kg, saccharomycete 14kg, green alga 12kg, zeolite 90kg and auxiliary material 10kg, the auxiliary material are acetic acid
The mass ratio of sodium and cornstarch, sodium acetate and cornstarch is 1:0.8.
Preparation method of the present embodiment for the complex microorganism water purification agent of aquaculture, includes the following steps:
(1) it is weighed respectively according to the weight of each raw material spare；
(2) spare bacillus subtilis, clostridium butyricum, bacillus cereus and saccharomycete are mixed, withered grass bud
Spore bacillus, clostridium butyricum, the gross mass of bacillus cereus and saccharomycete and water mass ratio be 1:3, be added cornstarch and
Water stirs evenly, and carries out fermented and cultured and obtains microbial fermentation solution, the temperature of fermentation is 30 DEG C, and fermentation time is 4 days, through examining
Total viable count in micrometer bio-fermented liquid is greater than 30,000,000,000/g, spare；
(3) spare zeolite is crushed, is ground up, sieved the processing of 60 meshes；
(4) microbial fermentation solution is added in 7 times of water and is diluted, then be added step (3) processing zeolite,
Spare auxiliary material and green alga uniformly mixes, and obtains the complex microorganism water purification agent for aquaculture.
Embodiment 2
The complex microorganism water purification agent for aquaculture of the present embodiment, including bacillus subtilis 20kg, butyric acid shuttle
Bacterium 34kg, bacillus cereus 24kg, saccharomycete 16kg, green alga 14kg, zeolite 94kg and auxiliary material 14kg, the auxiliary material are acetic acid
The mass ratio of sodium and cornstarch, sodium acetate and cornstarch is 1:0.9.
Preparation method of the present embodiment for the complex microorganism water purification agent of aquaculture, includes the following steps:
(1) it is weighed respectively according to the weight of each raw material spare；
(2) spare bacillus subtilis, clostridium butyricum, bacillus cereus and saccharomycete are mixed, withered grass bud
Spore bacillus, clostridium butyricum, bacillus cereus and the gross mass of saccharomycete and the mass ratio of water are 1:3.2, and cornstarch is added
And water, it stirring evenly, carries out fermented and cultured and obtain microbial fermentation solution, the temperature of fermentation is 32 DEG C, and fermentation time is 3.2 days,
It is greater than 30,000,000,000/g through the total viable count in detection microbial fermentation solution, it is spare；
(3) spare zeolite is crushed, is ground up, sieved the processing of 70 meshes；
(4) microbial fermentation solution is added in 7.2 times of water and is diluted, the boiling of step (3) processing is then added
Stone, spare auxiliary material and green alga uniformly mix, and obtain the complex microorganism water purification agent for aquaculture.
Embodiment 3
The complex microorganism water purification agent for aquaculture of the present embodiment, including bacillus subtilis 22kg, butyric acid shuttle
Bacterium 38kg, bacillus cereus 28kg, saccharomycete 18kg, green alga 16kg, zeolite 96kg and auxiliary material 16kg, the auxiliary material are acetic acid
The mass ratio of sodium and cornstarch, sodium acetate and cornstarch is 1:1.0.
Preparation method of the present embodiment for the complex microorganism water purification agent of aquaculture, includes the following steps:
(1) it is weighed respectively according to the weight of each raw material spare；
(2) spare bacillus subtilis, clostridium butyricum, bacillus cereus and saccharomycete are mixed, withered grass bud
Spore bacillus, clostridium butyricum, bacillus cereus and the gross mass of saccharomycete and the mass ratio of water are 1:3.5, and cornstarch is added
And water, it stirring evenly, carries out fermented and cultured and obtain microbial fermentation solution, the temperature of fermentation is 32 DEG C, and fermentation time is 3.5 days,
It is greater than 30,000,000,000/g through the total viable count in detection microbial fermentation solution, it is spare；
(3) spare zeolite is crushed, is ground up, sieved the processing of 75 meshes；
(4) microbial fermentation solution is added in 7.5 times of water and is diluted, the boiling of step (3) processing is then added
Stone, spare auxiliary material and green alga uniformly mix, and obtain the complex microorganism water purification agent for aquaculture.
Embodiment 4
The complex microorganism water purification agent for aquaculture of the present embodiment, including bacillus subtilis 24kg, butyric acid shuttle
Bacterium 45kg, bacillus cereus 30kg, saccharomycete 20kg, green alga 18kg, zeolite 100kg and auxiliary material 20kg, the auxiliary material are second
The mass ratio of sour sodium and cornstarch, sodium acetate and cornstarch is 1:1.2.
Preparation method of the present embodiment for the complex microorganism water purification agent of aquaculture, includes the following steps:
(1) it is weighed respectively according to the weight of each raw material spare；
(2) spare bacillus subtilis, clostridium butyricum, bacillus cereus and saccharomycete are mixed, withered grass bud
Spore bacillus, clostridium butyricum, the gross mass of bacillus cereus and saccharomycete and water mass ratio be 1:4, be added cornstarch and
Water stirs evenly, and carries out fermented and cultured and obtains microbial fermentation solution, the temperature of fermentation is 35 DEG C, and fermentation time is 3 days, through examining
Total viable count in micrometer bio-fermented liquid is greater than 30,000,000,000/g, spare；
(3) spare zeolite is crushed, is ground up, sieved the processing of 90 meshes；
(4) microbial fermentation solution is added in 8 times of water and is diluted, then be added step (3) processing zeolite,
Spare auxiliary material and green alga uniformly mixes, and obtains the complex microorganism water purification agent for aquaculture.
Comparative example 1
The raw material and preparation method of the complex microorganism water purification agent for aquaculture of this comparison are same as Example 1,
Difference does not add clostridium butyricum.
Comparative example 2
The raw material and preparation method of the complex microorganism water purification agent for aquaculture of this comparison are same as Example 1,
Difference does not add green alga.
Comparative example 3
The raw material and preparation method of the complex microorganism water purification agent for aquaculture of this comparison are same as Example 1,
Difference does not add zeolite.
Test example 1
Measurement in simulation pond to degrading nitrite
5 sterile screw socket bottles are taken, shrimp feed 2g/L, ammonium chloride is added in the pond water of dress about 1L cultivation South America prawn
0.051g/L, sodium nitrite 0.032g/L, 115 DEG C sterilize 30 minutes, wherein being separately added into embodiment 1 and comparative example in four bottles
The water purification agent 0.5g/L of 1-3 preparation is test group 1-4, and it is control group that any cleanser, which is not added, in another bottle as a control group
1, it at 30 DEG C, in constant incubator, stands, while time sampling measures content of nitrite, nitrite uses N- (1- naphthalene
Base) measurement of-ethylenediamine spectrophotometry, the degradation rate of nitrite is shown in Table 1.
Table 1
| Time | Test group 1 | Test group 2 | Test group 3 | Test group 4 | Control group 1 |
| 1 day | 31% | 14% | 16% | 12% | 1% |
| 3 days | 68% | 42% | 34% | 36% | 1% |
| 5 days | 84% | 53% | 46% | 45% | 1% |
| 7 days | 98% | 61% | 57% | 53% | 2% |
From table 1 it follows that the degradation rate of the nitrous acid of test group 1 be higher than test group 2-4 and control group 1, in this way by
The synergistic effect of each raw material, indispensable in the cleanser of test group, and lacking a kind of raw material apparent will influence nitrous acid
Degradation rate.
Above-mentioned test has also been made to other embodiments in the applicant, as a result almost the same, since length is limited, not one by one
It lists.
Test example 2
Five block sizes and the identical culture of Penaeus vannamei pond of water quality (2m × 2m × 1.2m) are taken to be tested, often
Join in Penaeus Vannmei seedling 100 of a length of 1-1.2cm or so in block pond, the water purification of one of preparation of embodiment 1 of splashing
Agent is test group 1, in addition the water purification agent of three pieces of comparative example 1-3 preparations of splashing, and remaining one piece is not launched any water purification agent, as
Blank control group 1.After 7 days, water-quality determination is carried out to all culture of Penaeus vannamei ponds, the foundation of water quality measurement is
GB11607-89 National Standard of the People's Republic of China water quality standard for fishery and NY5051-2001 pollution-free food freshwater aquiculture are used
Water water quality, testing index and data are as shown in table 2.
Table 2
As can be seen from the above table, every water quality indicator of test group 1 is superior to test group 2-4, becomes apparent from better than control group
1, illustrate that the purifying water effect of water purification agent of the invention is more preferable, needs the coordinated of each raw material excellent using just having the effect of.
Above-mentioned test has also been made to other embodiments in the applicant, as a result almost the same, since length is limited, not one by one
It lists.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of complex microorganism water purification agent for aquaculture, which is characterized in that according to parts by weight, the composite microbial
Object water purification agent includes bacillus subtilis 18-24 parts by weight, clostridium butyricum 25-45 parts by weight, bacillus cereus 20-30 weight
Part, saccharomycete 14-20 parts by weight, green alga 12-18 parts by weight, zeolite 90-100 parts by weight and auxiliary material 10-20 parts by weight.
2. the complex microorganism water purification agent according to claim 1 for aquaculture, which is characterized in that according to weight
Part, the complex microorganism water purification agent includes bacillus subtilis 20-22 parts by weight, clostridium butyricum 34-38 parts by weight, waxy
Bacillus 24-28 parts by weight, saccharomycete 16-18 parts by weight, green alga 14-16 parts by weight, zeolite 94-96 parts by weight and auxiliary material
14-16 parts by weight.
3. the complex microorganism water purification agent according to claim 1 or 2 for aquaculture, which is characterized in that described
Auxiliary material is sodium acetate and cornstarch.
4. the complex microorganism water purification agent according to claim 3 for aquaculture, which is characterized in that sodium acetate and jade
The mass ratio of rice starch is 1:0.8-1.2.
5. the preparation method described in a kind of claim 1-4 any one for the complex microorganism water purification agent of aquaculture,
It is characterized in that, the method includes the following steps:
(1) it is weighed respectively according to the weight of each raw material spare；
(2) spare bacillus subtilis, clostridium butyricum, bacillus cereus and saccharomycete are mixed, corn is added and forms sediment
Powder and water, stir evenly, and carry out fermented and cultured and obtain microbial fermentation solution, spare；
(3) spare zeolite is crushed, is ground up, sieved processing；
(4) microbial fermentation solution is added to the water dilution, the zeolite, spare auxiliary of step (3) processing is then added
Material and green alga uniformly mix, and obtain the complex microorganism water purification agent for aquaculture.
6. the preparation method of the complex microorganism water purification agent according to claim 5 for aquaculture, which is characterized in that
The temperature fermented in step (2) is 30-35 DEG C, and fermentation time is 3-4 days.
7. the preparation method of the complex microorganism water purification agent according to claim 6 for aquaculture, which is characterized in that
The temperature fermented in step (2) is 32 DEG C, and fermentation time is 3.5 days.
8. the preparation method of the complex microorganism water purification agent according to claim 6 for aquaculture, which is characterized in that
Bacillus subtilis, clostridium butyricum, bacillus cereus and the gross mass of saccharomycete and the mass ratio of water are 1:3- in step (2)
4。
9. the preparation method of the complex microorganism water purification agent according to claim 5 for aquaculture, which is characterized in that
The processing of 60-90 mesh is crossed in step (3).
10. the preparation method of the complex microorganism water purification agent according to claim 5 for aquaculture, feature exist
In the volume ratio of microbial fermentation solution and water is 1:7-8 in step (4).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111661933A (en) * | 2020-06-30 | 2020-09-15 | 武汉合缘绿色生物股份有限公司 | Biological agent for adjusting water body nutrition and preventing diseases and preparation method thereof |
| CN112481164A (en) * | 2020-12-01 | 2021-03-12 | 清远海贝生物技术有限公司 | Litopenaeus vannamei growth promoting microecological preparation and use method and application thereof |
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