CN104673695A - Preparation method of aquiculture water body purification microbial inoculant - Google Patents
Preparation method of aquiculture water body purification microbial inoculant Download PDFInfo
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- CN104673695A CN104673695A CN201310638226.XA CN201310638226A CN104673695A CN 104673695 A CN104673695 A CN 104673695A CN 201310638226 A CN201310638226 A CN 201310638226A CN 104673695 A CN104673695 A CN 104673695A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Environmental & Geological Engineering (AREA)
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- Fodder In General (AREA)
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Abstract
The invention discloses a preparation method of an aquiculture water body purification microbial inoculant. The method comprises the following steps: uniformly mixing 30-60 parts of composite microbial bacterium solution with 30-100 parts of auxiliary material, culturing at 28-35 DEG C for 6-24 hours, drying the culture at room temperature, granulating, and packaging. The composite microbial bacterium solution is one or mixture of more of Bacillus subtilis solution, Bacillus lateraporus solution, Lactobacillus acidophilus solution and microzyme solution, and the effective viable count of each bacterium solution is 10<6>10<8>/ml; and the auxiliary material is one or mixture of more of bentonite, bean pulp and corn flour. The method is simple in steps, does not need special equipment, and can easily implement industrialization. The water purifying agent prepared by the method has favorable degradation actions on ammonia nitrogen, nitrites and other pollutants in the aquiculture water body, and is capable of effectively inhibiting the pathogenic microorganism from growth, reducing the pathogens and enhancing the disease resistance of the aquiculture body.
Description
Technical field
The present invention relates to a kind of aquaculture system water purification microbial inoculum and preparation method thereof.
Background technology
Along with the raising of health of people consciousness, the demand of people to fishery products also constantly increases, and China's culture fishery is also in fast development thereupon.But in the tradition nursing process of aquaculture body, due to too low, the residual bait of bait utilization, cultivated animals defecation, the eutrophication that seawater is muddy, cause seawater can be made, thus cause hydroplankton amount reproduction, destroy the growing environment of cultivation body, cultivation bulk-growth speed is slowly and easily ill.In addition, in the input of long-term, a large amount of bait, cause body eutrophication, in water body, ammonia nitrogen and nitrite exceed standard, and produce toxic action to water body animal.
Microbial preparation is with its advantage such as toxicological harmless, noresidue, the concern being subject to people.Utilize the metabolism of microorganism, reduce ammonia nitrogen, the hydrogen sulfide content of aquaculture water, purify water.Some microbial preparation can promote the conversion of feed, enables cultivated animals effectively absorb its nutritive ingredient, promotes the growth of cultivation body.Had a variety of microbe application in the purification of aquaculture water at present, have also been obtained good effect, but in order to obtain better dewatering effect, based on the aquaculture water scavenging agent of the feature of aquaculture water own, need more deep research and development.
Summary of the invention
The object of the present invention is to provide a kind of aquaculture system water purification microbial inoculum and preparation method thereof.
Technical scheme of the present invention is as follows:
A preparation method for aquaculture system water purification microbial inoculum, comprises the steps:
(1) subtilis, bacillus laterosporus, Lactobacterium acidophilum are inoculated in LB liquid nutrient medium respectively, cultivate 16-48 hour at 28-35 DEG C of shaking table, obtain subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid respectively; Yeast is inoculated in PDA substratum, cultivates 16-48 hour, obtain yeast liquid at 28-30 DEG C of shaking table;
(2) the subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid and the yeast liquid that step (1) are obtained by volume 1:1:1:1 mix, and obtain composite microbial bacteria liquid;
(3) get 30-60 part composite microbial bacteria liquid and 30-100 part auxiliary material mixes, cultivate 6-24 hour at 28-35 DEG C;
(4) by the culture drying at room temperature of step (3), particle is made, packing.
In the present invention, in the subtilis liquid described in step (1), bacillus laterosporus liquid, Lactobacterium acidophilum liquid and yeast liquid, living bacteria count is respectively 10
6~ 10
8individual/ml.
In the present invention, step (3) described auxiliary material is one or more the mixture in wilkinite, dregs of beans, corn flour.
LB substratum of the present invention and PDA substratum are commercially available product.
The beneficial effect of the invention: method steps of the present invention simply, does not need special equipment, is easy to industrialization.Water purification agent prepared by method of the present invention has good Degradation to pollutents such as the ammonia nitrogen of aquaculture system and nitrite, effectively can suppress the growth of pathogenic micro-organism, reduces pathogenic agent, increases the diseases prevention ability of aquaculture body.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.Test method described in embodiment, if no special instructions, is ordinary method; Described reagent and material, if no special instructions, all can obtain from commercial channels, or can prepare in conventional manner.
Embodiment 1
A preparation method for aquaculture system water purification microbial inoculum, comprises the steps:
(1) be inoculated in by subtilis in 100ml LB liquid medium, cultivate 24 hours at 30 DEG C, obtaining living bacteria count is 3 × 10
7the subtilis liquid of individual/ml;
(2) be inoculated in by bacillus laterosporus in 100ml LB liquid medium, cultivate 16 hours at 28 DEG C, obtaining living bacteria count is 2 × 10
7the bacillus laterosporus liquid of individual/ml;
(3) be inoculated in by Lactobacterium acidophilum in 100ml LB liquid medium, cultivate 30 hours at 28 DEG C, obtaining living bacteria count is 1.5 × 10
7the Lactobacterium acidophilum liquid of individual/ml;
(4) be inoculated in by yeast in 100ml liquid PDA liquid nutrient medium, cultivate 30 hours at 30 DEG C, obtaining living bacteria count is 3 × 10
7the yeast liquid of individual/ml;
(5) get subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid that step (1)-(4) obtain by 100ml respectively, mix, obtain composite microbial bacteria liquid;
(6) mixed by the composite microbial bacteria liquid that 400g wilkinite, 100g dregs of beans and step (5) obtain, cultivate 10 hours at 30 DEG C, culture drying at room temperature, makes particle, packing.
Embodiment 2
A preparation method for aquaculture system water purification microbial inoculum, comprises the steps:
(1) be inoculated in by subtilis in 100ml LB liquid medium, cultivate 24 hours at 30 DEG C, obtaining living bacteria count is 3 × 10
7the subtilis liquid of individual/ml;
(2) be inoculated in by bacillus laterosporus in 100ml LB liquid medium, cultivate 16 hours at 28 DEG C, obtaining living bacteria count is 2 × 10
7the bacillus laterosporus liquid of individual/ml;
(3) be inoculated in by Lactobacterium acidophilum in 100ml LB liquid medium, cultivate 30 hours at 28 DEG C, obtaining living bacteria count is 1.5 × 10
7the Lactobacterium acidophilum liquid of individual/ml;
(4) be inoculated in by yeast in 100ml liquid PDA liquid nutrient medium, cultivate 30 hours at 30 DEG C, obtaining living bacteria count is 3 × 10
7the yeast liquid of individual/ml;
(5) get subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid that step (1)-(4) obtain by 100ml respectively, mix, obtain composite microbial bacteria liquid;
(6) mixed by the composite microbial bacteria liquid that 400g wilkinite and step (5) obtain, cultivate 12 hours at 30 DEG C, culture drying at room temperature, makes particle, packing.
Claims (3)
1. a preparation method for aquaculture system water purification microbial inoculum, comprises the steps:
(1) subtilis, bacillus laterosporus, Lactobacterium acidophilum are inoculated in LB liquid nutrient medium respectively, cultivate 16-48 hour at 28-35 DEG C of shaking table, obtain subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid respectively; Yeast is inoculated in PDA substratum, cultivates 16-48 hour, obtain yeast liquid at 28-30 DEG C of shaking table;
(2) the subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid and the yeast liquid that step (1) are obtained by volume 1:1:1:1 mix, and obtain composite microbial bacteria liquid;
(3) get 30-60 part composite microbial bacteria liquid and 30-100 part auxiliary material mixes, cultivate 6-24 hour at 28-35 DEG C;
(4) by the culture drying at room temperature of step (3), particle is made, packing.
2. preparation method according to claim 1, is characterized in that, in the subtilis liquid described in step (1), bacillus laterosporus liquid, Lactobacterium acidophilum liquid and yeast liquid, living bacteria count is respectively 10
6~ 10
8individual/ml.
3. preparation method according to claim 1, is characterized in that, step (3) described auxiliary material is one or more the mixture in wilkinite, dregs of beans, corn flour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310638226.XA CN104673695A (en) | 2013-11-29 | 2013-11-29 | Preparation method of aquiculture water body purification microbial inoculant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310638226.XA CN104673695A (en) | 2013-11-29 | 2013-11-29 | Preparation method of aquiculture water body purification microbial inoculant |
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|---|---|
| CN104673695A true CN104673695A (en) | 2015-06-03 |
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| CN201310638226.XA Pending CN104673695A (en) | 2013-11-29 | 2013-11-29 | Preparation method of aquiculture water body purification microbial inoculant |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106418003A (en) * | 2016-10-11 | 2017-02-22 | 上海高龙生物科技有限公司 | Micro-ecological preparation combining water purification function for aquatic animals |
| CN106587381A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
| CN109133368A (en) * | 2018-09-13 | 2019-01-04 | 无锡跃洋生物科技有限公司 | A kind of complex microorganism water purification agent and preparation method thereof for aquaculture |
| CN114262065A (en) * | 2021-11-19 | 2022-04-01 | 惠州学院 | Micro-ecological preparation for purifying aquaculture tail water |
-
2013
- 2013-11-29 CN CN201310638226.XA patent/CN104673695A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106418003A (en) * | 2016-10-11 | 2017-02-22 | 上海高龙生物科技有限公司 | Micro-ecological preparation combining water purification function for aquatic animals |
| CN106418003B (en) * | 2016-10-11 | 2019-07-02 | 上海国龙生物技术集团有限公司 | A kind of aquatic livestock probiotics having both water purification effect |
| CN106587381A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
| CN109133368A (en) * | 2018-09-13 | 2019-01-04 | 无锡跃洋生物科技有限公司 | A kind of complex microorganism water purification agent and preparation method thereof for aquaculture |
| CN114262065A (en) * | 2021-11-19 | 2022-04-01 | 惠州学院 | Micro-ecological preparation for purifying aquaculture tail water |
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Application publication date: 20150603 |