CN106587381A - Composite microorganism water purifying agent for sea cucumber aquaculture water - Google Patents
Composite microorganism water purifying agent for sea cucumber aquaculture water Download PDFInfo
- Publication number
- CN106587381A CN106587381A CN201611184511.9A CN201611184511A CN106587381A CN 106587381 A CN106587381 A CN 106587381A CN 201611184511 A CN201611184511 A CN 201611184511A CN 106587381 A CN106587381 A CN 106587381A
- Authority
- CN
- China
- Prior art keywords
- liquid
- bacterium solution
- bacillus subtilis
- water
- sea cucumber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
The invention discloses a composite microorganism water purifying agent for sea cucumber aquaculture water. The composite microorganism water purifying agent for the sea cucumber aquaculture water comprises, by mass, 40 parts of composite microorganism bacterium liquid and 60 parts of auxiliary materials, wherein the composite microorganism bacterium liquid is one or a mixture of bacillus subtilis liquid, bacillus subtilis liquid and lactobacillus acidophilus liquid; the effective viable bacterium number of each bacterium liquid is 108-109/ml; and the auxiliary materials are one or a mixture of diatomite, rice husk powder and corn cob powder. The water purifying agent has a good degradation effect on ammonia nitrogen, nitrite and other pollutants of the sea cucumber aquaculture water, can well inhibit growth of pathogenic microorganisms, reduces pathogens and improves the anti-disease capacity of sea cucumbers. The composite microorganism water purifying agent for the sea cucumber aquaculture water is reasonably matched with bacterium strains which are high in water purifying efficiency and stable in performance, the function complementary role of the bacterium strains is played to the maximum extent, the formula is reasonable, price is low, and popularization is facilitated.
Description
Technical field
The present invention relates to a kind of breeding water body water purification agent, more particularly, to a kind of holothruian cultures water body complex microorganism
Water purification agent.
Background technology
In recent years, the wilderness demand in the nutritive value of sea cucumber and market, sea cucumber propagates industry fast development artificially.At present by
During the tradition of sea cucumber is fed, because bait utilization is too low, residual bait, cultivated animals defecation can make seawater muddiness, cause
The eutrophication of seawater, so as to cause hydroplankton amount reproduction, destroys the growing environment of sea cucumber, thus sea cucumber growth is fast
Spend slow and easily ill.In addition, the increase of water body eutrophication degree, causes ammonia nitrogen and nitrite exceeded, water body animal is produced
Raw toxic action.So, the eutrophication and ammonia and nitrogen pollution for solving the problems, such as breeding water body is the heat subject of aquatic products industry.
Microorganism formulation the advantages of its nonhazardous, noresidue, by people being paid close attention to.Made using the metabolism of microorganism
With, ammonia nitrogen, the hydrogen sulfide content of breeding water body are reduced, purify water.Some microorganism formulations can promote the conversion of feed, make
Cultivated animals can effectively absorb its nutrient content, promote the growth of cultivation body.Had at present a variety of microbe applications in
The purification of breeding water body, has also obtained preferable effect, but in order to obtain more preferable dewatering effect, it is special based on breeding water body itself
Point breeding water body cleanser, need deeper into research and development.
The content of the invention
It is an object of the invention to provide a kind of microbial water-purifying agent for being suitable for holothruian cultures water body.
The technical scheme is that as follows：
A kind of holothruian cultures water body complex microorganism water purification agent, by mass parts following component is included：Composite microbial bacteria
40 parts of liquid, 60 parts of auxiliary material, wherein the composite microbial bacteria liquid is bacillus subtilis bacterium solution, bacillus licheniformis liquid, acidophilus breast
The mixture of one or more in bacillus liquid, yeast liquid, each bacterium solution living bacteria count is 108～1010Individual/ml；It is described auxiliary
Expect the mixture for one or more in diatomite, powdered rice hulls, maize cob meal.
In the present invention, bacillus subtilis bacterium solution, bacillus licheniformis, lactobacillus acidophilus in the complex microorganism bacterium solution
Liquid, the mass ratio of yeast liquid are 1:1:1:1.
In the present invention, the complex microorganism bacterium solution is obtained with the following method：Bacillus subtilis, bacillus licheniformis,
Lactobacillus acidophilus is inoculated in respectively in LB fluid nutrient mediums, in 28-35 DEG C of shaking table culture 24-32 hour, withered grass gemma is obtained respectively
Bacillus liquid, bacillus licheniformis liquid, lactobacillus acidophilus liquid；Saccharomycete is inoculated in PDA culture medium, in 28-30 DEG C of shaking table culture
24-32 hours, obtain yeast liquid；Mixing bacillus subtilis bacterium solution, bacillus licheniformis liquid, lactobacillus acidophilus liquid, yeast liquid
In one or more.
The LB culture mediums and PDA culture medium are commercially available product.
The preparation method of holothruian cultures microorganism water water purification agent of the present invention is：What is obtained as stated above is compound micro-
40 parts of biological bacterium solution is added in 60 parts of auxiliary material and is well mixed, 30-35 DEG C cultivate 24-32 hours, culture low temperature drying,
Particle is made, is dispensed.
The beneficial effect of the invention：The water purification agent of the present invention has to pollutants such as the ammonia nitrogens and nitrite of holothruian cultures water body
Well degradation, can effectively suppress the growth of pathogenic microorganism, reduce pathogen, increase the diseases prevention ability of sea cucumber.This
Bright reasonable coordination cleaning water is high, the bacterial strain of stable performance, realizes that the bacterial strain effect of having complementary functions obtains the performance of maximum, and formula is closed
Reason, it is cheap, be beneficial to promote.
Specific embodiment
Following non-limiting examples can make one of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.Test method described in embodiment, if no special instructions, is conventional method；The reagent and
Material, if no special instructions, commercially obtains, or can prepare in conventional manner.
Embodiment 1
A kind of holothruian cultures water body complex microorganism water purification agent, including complex microorganism bacterium solution and auxiliary material, wherein described
Complex microorganism bacterium solution is bacillus subtilis bacterium solution, bacillus licheniformis liquid, lactobacillus acidophilus liquid, the mixture of yeast liquid；
The auxiliary material is the mixture of diatomite and powdered rice hulls.The water purification agent is prepared as follows：
(1) bacillus subtilis is inoculated in 100ml LB liquid mediums, is cultivated 24 hours at 30 DEG C, obtained and effectively live
Bacterium number is 2 × 109The bacillus subtilis bacterium solution of individual/ml；
(2) bacillus licheniformis is inoculated in 100ml LB liquid mediums, is cultivated 24 hours at 28 DEG C, obtained and effectively live
Bacterium number is 3 × 109The bacillus licheniformis liquid of individual/ml；
(3) lactobacillus acidophilus is inoculated in 100ml LB liquid mediums, is cultivated 32 hours at 28 DEG C, obtain effective viable bacteria
Number is 1.7 × 109The lactobacillus acidophilus liquid of individual/ml；
(4) saccharomycete is inoculated in 100ml liquid PDA liquid mediums, is cultivated 32 hours at 32 DEG C, obtain effective viable bacteria
Number is 4 × 109The yeast liquid of individual/ml；
(5) bacillus subtilis bacterium solution, bacillus licheniformis liquid, acidophilus breast that 80ml obtains step (1)-(4) are taken respectively
Bacillus liquid, yeast liquid in mass ratio 1:1:1:1 is well mixed, and obtains complex microorganism bacterium solution；
(6) the complex microorganism bacterium solution that 300g diatomite, 100g powdered rice hulls and step (5) are obtained is well mixed, 30
DEG C culture 24 hours, particle is made in culture low temperature drying, packing.
Embodiment 2
A kind of holothruian cultures microorganism water water purification agent, including complex microorganism bacterium solution and auxiliary material, wherein described compound
Microbial inoculum is bacillus subtilis bacterium solution, bacillus licheniformis liquid, the mixture of yeast liquid；The auxiliary material is diatomite.
The water purification agent is prepared as follows：
(1) bacillus subtilis is inoculated in 100ml LB liquid mediums, is cultivated 24 hours at 30 DEG C, obtained and effectively live
Bacterium number is 2 × 109The bacillus subtilis bacterium solution of individual/ml；
(2) bacillus licheniformis is inoculated in 100ml LB liquid mediums, is cultivated 24 hours at 28 DEG C, obtained and effectively live
Bacterium number is 3 × 109The bacillus licheniformis liquid of individual/ml；
(3) lactobacillus acidophilus is inoculated in 100ml liquid PDA liquid mediums, is cultivated 30 hours at 32 DEG C, obtained effectively
Viable count is 4 × 109The yeast liquid of individual/ml；
(4) saccharomycete is inoculated in 100ml liquid PDA liquid mediums, is cultivated 32 hours at 32 DEG C, obtain effective viable bacteria
Number is 4 × 109The yeast liquid of individual/ml；
(5) bacillus subtilis bacterium solution, bacillus licheniformis liquid, acidophilus breast that 80ml obtains step (1)-(4) are taken respectively
Bacillus liquid, yeast liquid in mass ratio 1:1:1:1 is well mixed, and obtains complex microorganism bacterium solution；
(6) the complex microorganism bacterium solution that 300g diatomite, 100g maize cob meals and step (5) are obtained is well mixed,
30 DEG C are cultivated 24 hours, and particle is made in culture low temperature drying, packing.
Embodiment 3
A kind of holothruian cultures microorganism water water purification agent, including complex microorganism bacterium solution and auxiliary material, wherein described compound
Microbial inoculum is bacillus subtilis bacterium solution, bacillus licheniformis liquid, the mixture of yeast liquid；The auxiliary material is diatomite.
The water purification agent is prepared as follows：
(1) bacillus subtilis is inoculated in 100ml LB liquid mediums, is cultivated 24 hours at 30 DEG C, obtained and effectively live
Bacterium number is 2 × 109The bacillus subtilis bacterium solution of individual/ml；
(2) bacillus licheniformis is inoculated in 100ml LB liquid mediums, is cultivated 24 hours at 28 DEG C, obtained and effectively live
Bacterium number is 3 × 109The bacillus licheniformis liquid of individual/ml；
(3) lactobacillus acidophilus is inoculated in 100ml liquid PDA liquid mediums, is cultivated 30 hours at 32 DEG C, obtained effectively
Viable count is 4 × 109The yeast liquid of individual/ml；
(4) saccharomycete is inoculated in 100ml liquid PDA liquid mediums, is cultivated 32 hours at 32 DEG C, obtain effective viable bacteria
Number is 4 × 109The yeast liquid of individual/ml；
(5) bacillus subtilis bacterium solution, bacillus licheniformis liquid, acidophilus breast that 80ml obtains step (1)-(4) are taken respectively
Bacillus liquid, yeast liquid in mass ratio 1:1:1:1 is well mixed, and obtains complex microorganism bacterium solution；
(6) the complex microorganism bacterium solution that 200g powdered rice hulls, 200g maize cob meals and step (5) are obtained is well mixed,
30 DEG C are cultivated 24 hours, culture low temperature drying, with powdery packing.
Claims (3)
1. a kind of holothruian cultures water body complex microorganism water purification agent, it is characterised in that include following component by mass parts：It is compound
40 parts of microbial inoculum, 60 parts of auxiliary material, wherein the composite microbial bacteria liquid is bacillus subtilis bacterium solution, bacillus licheniformis
The mixture of one or more in liquid, lactobacillus acidophilus liquid, yeast liquid, each bacterium solution living bacteria count is 108～1010Individual/
ml；The auxiliary material is the mixture of one or more in diatomite, powdered rice hulls, maize cob meal.
2. water purification agent according to claim 1, it is characterised in that bacillus subtilis in the complex microorganism bacterium solution
Liquid, bacillus licheniformis, lactobacillus acidophilus liquid, the mass ratio of yeast liquid are 1:1:1:1.
3. water purification agent according to claim 1 and 2, it is characterised in that the complex microorganism bacterium solution is obtained with the following method
：Bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus are inoculated in respectively in LB fluid nutrient mediums, in 28-35 DEG C of shaking table
Culture 24-32 hours, obtain respectively bacillus subtilis bacterium solution, bacillus licheniformis liquid, lactobacillus acidophilus liquid；Saccharomycete is inoculated in
In PDA culture medium, in 28-30 DEG C of shaking table culture 24-32 hour, yeast liquid is obtained；Mixing bacillus subtilis bacterium solution, lichens gemma
One or more in bacillus liquid, lactobacillus acidophilus liquid, yeast liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611184511.9A CN106587381A (en) | 2016-12-20 | 2016-12-20 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611184511.9A CN106587381A (en) | 2016-12-20 | 2016-12-20 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
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| Publication Number | Publication Date |
|---|---|
| CN106587381A true CN106587381A (en) | 2017-04-26 |
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Family Applications (1)
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| CN201611184511.9A Pending CN106587381A (en) | 2016-12-20 | 2016-12-20 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108483675A (en) * | 2018-05-03 | 2018-09-04 | 杨敏杰 | The preparation method of immobilization water purification agent and its purposes for handling aquatic herbaceous plant |
| CN108587964A (en) * | 2018-05-03 | 2018-09-28 | 杨敏杰 | Immobilization composite bacteria agent and its in water body water hyacinth control and application |
| CN110980967A (en) * | 2019-12-20 | 2020-04-10 | 广东慧信环保有限公司 | Microbial composite water purifying agent and preparation method thereof |
| CN115736137A (en) * | 2022-11-22 | 2023-03-07 | 杨凌铱安生物科技有限公司 | Multifunctional sea cucumber feed additive and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673695A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Preparation method of aquiculture water body purification microbial inoculant |
| CN104671427A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Microbial water purifying agent for sea cucumber cultivation water bodies |
-
2016
- 2016-12-20 CN CN201611184511.9A patent/CN106587381A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673695A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Preparation method of aquiculture water body purification microbial inoculant |
| CN104671427A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Microbial water purifying agent for sea cucumber cultivation water bodies |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108483675A (en) * | 2018-05-03 | 2018-09-04 | 杨敏杰 | The preparation method of immobilization water purification agent and its purposes for handling aquatic herbaceous plant |
| CN108587964A (en) * | 2018-05-03 | 2018-09-28 | 杨敏杰 | Immobilization composite bacteria agent and its in water body water hyacinth control and application |
| CN110980967A (en) * | 2019-12-20 | 2020-04-10 | 广东慧信环保有限公司 | Microbial composite water purifying agent and preparation method thereof |
| CN115736137A (en) * | 2022-11-22 | 2023-03-07 | 杨凌铱安生物科技有限公司 | Multifunctional sea cucumber feed additive and application thereof |
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Application publication date: 20170426 |
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