CN104671427A - Microbial water purifying agent for sea cucumber cultivation water bodies - Google Patents
Microbial water purifying agent for sea cucumber cultivation water bodies Download PDFInfo
- Publication number
- CN104671427A CN104671427A CN201310638214.7A CN201310638214A CN104671427A CN 104671427 A CN104671427 A CN 104671427A CN 201310638214 A CN201310638214 A CN 201310638214A CN 104671427 A CN104671427 A CN 104671427A
- Authority
- CN
- China
- Prior art keywords
- liquid
- solution
- subtilis
- water
- composite microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
Abstract
The invention discloses a microbial water purifying agent for sea cucumber cultivation water bodies, which comprises the following components in parts by mass: 30-40 parts of composite microbial bacterium solution and 30-80 parts of auxiliary material. The composite microbial bacterium solution is one or mixture of more of Bacillus subtilis solution, Bacillus lateraporus solution, Lactobacillus acidophilus solution and microzyme solution, and the effective viable count of each bacterium solution is 10<6>10<8>/ml; and the auxiliary material is one or mixture of more of bentonite, bean pulp and corn flour. The water purifying agent has favorable degradation actions on ammonia nitrogen, nitrites and other pollutants in the sea cucumber cultivation water body, and is capable of effectively inhibiting the pathogenic microorganism from growth, reducing the pathogens and enhancing the disease resistance of the sea cucumbers. The strains with high water purification efficiency and stable performance are reasonably compounded to maximally display the functional complementary actions of the strains; and the microbial water purifying agent has the advantages of reasonable formula and low price, and is suitable for popularization.
Description
Technical field
The present invention relates to a kind of aquaculture water water purification agent, especially relate to a kind of holothruian cultures microorganism water water purification agent.
Background technology
In this year, the nutritive value of sea cucumber and the heavy demand in market, sea cucumber propagates industry fast development artificially.At present due in the tradition nursing process of sea cucumber, due to too low, the residual bait of bait utilization, cultivated animals defecation, the eutrophication that seawater is muddy, cause seawater can be made, thus cause hydroplankton amount reproduction, destroy the growing environment of sea cucumber, thus the sea cucumber speed of growth is slowly and easily ill.In addition, the increase of water body eutrophication degree, causes ammonia nitrogen and nitrite to exceed standard, and produces toxic action to water body animal.So the eutrophication of solution aquaculture water and ammonia and nitrogen pollution problem are the heat subjects of water industry.
Microbial preparation is with its advantage such as toxicological harmless, noresidue, the concern being subject to people.Utilize the metabolism of microorganism, reduce ammonia nitrogen, the hydrogen sulfide content of aquaculture water, purify water.Some microbial preparation can promote the conversion of feed, enables cultivated animals effectively absorb its nutritive ingredient, promotes the growth of cultivation body.Had a variety of microbe application in the purification of aquaculture water at present, have also been obtained good effect, but in order to obtain better dewatering effect, based on the aquaculture water scavenging agent of the feature of aquaculture water own, need more deep research and development.
Summary of the invention
The object of the present invention is to provide a kind of microbial water-purifying agent being suitable for holothruian cultures water body.
Technical scheme of the present invention is as follows:
A kind of holothruian cultures microorganism water water purification agent, it is characterized in that, following component is comprised: composite microbial bacteria liquid 30-40 part, auxiliary material 30-80 part by mass parts, wherein said composite microbial bacteria liquid is one or more the mixture in subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid, and each bacterium liquid living bacteria count is 10
6~ 10
8individual/ml; Described auxiliary material is one or more the mixture in wilkinite, dregs of beans, corn flour.
In the present invention, in described composite microbial bacteria liquid, the mass ratio of subtilis liquid, bacillus laterosporus, Lactobacterium acidophilum liquid, yeast liquid is 1:1:1:1.
In the present invention, described composite microbial bacteria liquid obtains with the following method: subtilis, bacillus laterosporus, Lactobacterium acidophilum are inoculated in LB liquid nutrient medium respectively, cultivate 16-48 hour at 28-35 DEG C of shaking table, obtain subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid respectively; Yeast is inoculated in PDA substratum, cultivates 16-48 hour, obtain yeast liquid at 28-30 DEG C of shaking table; One or more in mixing subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid.
Described LB substratum and PDA substratum are commercially available product.
The preparation method of holothruian cultures microorganism water water purification agent of the present invention is: the composite microbial bacteria liquid 30-40 part obtained as stated above joins in the auxiliary material of 30-80 part and mixes, 16-48 hour is cultivated at 30-35 DEG C, culture cryodrying, makes particle, packing.
The beneficial effect of the invention: water purification agent of the present invention has good Degradation to pollutents such as the ammonia nitrogen of holothruian cultures water body and nitrite, effectively can suppress the growth of pathogenic micro-organism, reduces pathogenic agent, increases the diseases prevention ability of sea cucumber.Reasonable coordination cleaning water of the present invention is high, the bacterial strain of stable performance, realizes the bacterial strain effect of having complementary functions and obtains maximum performance, fill a prescription reasonable, cheap, be of value to popularization.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.Test method described in embodiment, if no special instructions, is ordinary method; Described reagent and material, if no special instructions, all can obtain from commercial channels, or can prepare in conventional manner.
Embodiment 1
A kind of holothruian cultures microorganism water water purification agent, comprise composite microbial bacteria liquid and auxiliary material, wherein said composite microbial bacteria liquid is the mixture of subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid; Described auxiliary material is the mixture of wilkinite and dregs of beans.
Described water purification agent is prepared as follows:
(1) be inoculated in by subtilis in 100ml LB liquid medium, cultivate 16 hours at 30 DEG C, obtaining living bacteria count is 2 × 10
7the subtilis liquid of individual/ml;
(2) be inoculated in by bacillus laterosporus in 100ml LB liquid medium, cultivate 24 hours at 28 DEG C, obtaining living bacteria count is 3 × 10
7the bacillus laterosporus liquid of individual/ml;
(3) be inoculated in by Lactobacterium acidophilum in 100ml LB liquid medium, cultivate 32 hours at 28 DEG C, obtaining living bacteria count is 1.7 × 10
7the Lactobacterium acidophilum liquid of individual/ml;
(4) be inoculated in by yeast in 100ml liquid PDA liquid nutrient medium, cultivate 30 hours at 32 DEG C, obtaining living bacteria count is 4 × 10
7the yeast liquid of individual/ml;
(5) get subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid that step (1)-(4) obtain by 80ml respectively, mix, obtain composite microbial bacteria liquid;
(6) mixed by the composite microbial bacteria liquid that 300g wilkinite, 100g dregs of beans and step (5) obtain, cultivate 24 hours at 30 DEG C, culture cryodrying, makes particle, packing.
Embodiment 2
A kind of holothruian cultures microorganism water water purification agent, a kind of holothruian cultures microorganism water water purification agent, comprise composite microbial bacteria liquid and auxiliary material, wherein said composite microbial bacteria liquid is the mixture of subtilis liquid, bacillus laterosporus liquid, yeast liquid; Described auxiliary material is wilkinite.
Described water purification agent is prepared as follows:
(1) be inoculated in by subtilis in 100ml LB liquid medium, cultivate 16 hours at 30 DEG C, obtaining living bacteria count is 2 × 10
7the subtilis liquid of individual/ml;
(2) be inoculated in by bacillus laterosporus in 100ml LB liquid medium, cultivate 24 hours at 28 DEG C, obtaining living bacteria count is 3 × 10
7the bacillus laterosporus liquid of individual/ml;
(3) be inoculated in by yeast in 100ml liquid PDA liquid nutrient medium, cultivate 30 hours at 32 DEG C, obtaining living bacteria count is 4 × 10
7the yeast liquid of individual/ml;
(4) get subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid that step (1)-(4) obtain by 80ml respectively, mix, obtain composite microbial bacteria liquid;
(5) mixed by the composite microbial bacteria liquid that 300g wilkinite and step (5) obtain, cultivate 24 hours at 30 DEG C, culture cryodrying, makes particle, packing.
Claims (3)
1. a holothruian cultures microorganism water water purification agent, it is characterized in that, following component is comprised: composite microbial bacteria liquid 30-40 part, auxiliary material 30-80 part by mass parts, wherein said composite microbial bacteria liquid is one or more the mixture in subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid, and each bacterium liquid living bacteria count is 10
6~ 10
8individual/ml; Described auxiliary material is one or more the mixture in wilkinite, dregs of beans, corn flour.
2. water purification agent according to claim 1, is characterized in that, in described composite microbial bacteria liquid, the mass ratio of subtilis liquid, bacillus laterosporus, Lactobacterium acidophilum liquid, yeast liquid is 1:1:1:1.
3. water purification agent according to claim 1 and 2, it is characterized in that, described composite microbial bacteria liquid obtains with the following method: subtilis, bacillus laterosporus, Lactobacterium acidophilum are inoculated in LB liquid nutrient medium respectively, cultivate 16-48 hour at 28-35 DEG C of shaking table, obtain subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid respectively; Yeast is inoculated in PDA substratum, cultivates 16-48 hour, obtain yeast liquid at 28-30 DEG C of shaking table; One or more in mixing subtilis liquid, bacillus laterosporus liquid, Lactobacterium acidophilum liquid, yeast liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310638214.7A CN104671427A (en) | 2013-11-29 | 2013-11-29 | Microbial water purifying agent for sea cucumber cultivation water bodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310638214.7A CN104671427A (en) | 2013-11-29 | 2013-11-29 | Microbial water purifying agent for sea cucumber cultivation water bodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104671427A true CN104671427A (en) | 2015-06-03 |
Family
ID=53307089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310638214.7A Pending CN104671427A (en) | 2013-11-29 | 2013-11-29 | Microbial water purifying agent for sea cucumber cultivation water bodies |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104671427A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105540867A (en) * | 2015-12-14 | 2016-05-04 | 中国水产科学研究院黄海水产研究所 | Formula of floc nutrients for cultivating bio-flocs and beneficial bacteria as well as preparation and application |
| CN106587381A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
| CN108157668A (en) * | 2017-11-17 | 2018-06-15 | 辽宁景良生物科技开发有限公司 | A kind of used in mariculture probiotics and its preparation method and application |
| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
-
2013
- 2013-11-29 CN CN201310638214.7A patent/CN104671427A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105540867A (en) * | 2015-12-14 | 2016-05-04 | 中国水产科学研究院黄海水产研究所 | Formula of floc nutrients for cultivating bio-flocs and beneficial bacteria as well as preparation and application |
| CN106587381A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
| CN108157668A (en) * | 2017-11-17 | 2018-06-15 | 辽宁景良生物科技开发有限公司 | A kind of used in mariculture probiotics and its preparation method and application |
| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101891511B (en) | Organic substrate suitable for planting vegetables, melons and fruits and preparation method thereof | |
| CN101717301B (en) | Capsule type microbial fertilizer and preparation method thereof | |
| CN110282755A (en) | A kind of formula and preparation method thereof of water remediation microbial inoculum | |
| CN102250768A (en) | Method for preparing enzyme and bacterium composite agent for treating sewage and sludge | |
| CN103773722B (en) | Salt tolerant also has subtilis and the application thereof of low-temperature biological deamination function | |
| CN102352316B (en) | Composite germ pulp, and production method and application thereof | |
| CN104152374A (en) | Microbial preparation for purifying breeding wastewater | |
| CN103319236B (en) | Microorganism fish guano and preparation method thereof | |
| CN101643280B (en) | Microbial water-purifying agent and preparation method thereof | |
| CN102674563A (en) | Novel environment-friendly efficient microorganism water quality purifying mixture and preparation method thereof | |
| CN109607819A (en) | A kind of control of microorganisms aquaculture method | |
| CN104176834A (en) | Freshwater compound microorganism substrate modifier | |
| CN103693752A (en) | Holothurian culture water quality restoration agent for effectively removing water eutrophication and preparation method and application thereof | |
| CN109280628B (en) | Enterobacter strain and application thereof in promoting growth of moso bamboos | |
| CN103689287B (en) | Microbial fish guano and preparation method thereof | |
| CN104671427A (en) | Microbial water purifying agent for sea cucumber cultivation water bodies | |
| CN106587381A (en) | Composite microorganism water purifying agent for sea cucumber aquaculture water | |
| CN105646015A (en) | Composite microelement fertilizer containing chlorella and paenibacillus, preparation and applications thereof | |
| JP2014533499A (en) | Cleaning liquid | |
| CN104673695A (en) | Preparation method of aquiculture water body purification microbial inoculant | |
| CN102399699B (en) | Method for producing biological water-purifying agent through microbe mutual fermentation of chicken manure | |
| CN108423838B (en) | Microbial preparation for ecological safety water system and preparation method thereof | |
| CN103693755B (en) | The single stage method removal of impurity, the holothruian cultures aquifer amendment suppressing harmful microorganism breeding and its production and use | |
| CN102850121A (en) | Environment-friendly biological ferment fertilizer | |
| KR101259416B1 (en) | The manufacturing method of probiotics containing mixed strains bm-s-1 for soil contamination purification or soil environmental improvement and manufactured probiotics thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150603 |