CN101643280B - Microbial water-purifying agent and preparation method thereof - Google Patents

Microbial water-purifying agent and preparation method thereof Download PDF

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CN101643280B
CN101643280B CN2009100660227A CN200910066022A CN101643280B CN 101643280 B CN101643280 B CN 101643280B CN 2009100660227 A CN2009100660227 A CN 2009100660227A CN 200910066022 A CN200910066022 A CN 200910066022A CN 101643280 B CN101643280 B CN 101643280B
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张培举
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Abstract

The invention relates to a microbial water-purifying agent and a preparation method thereof. The microbial water-purifying agent is prepared by fermenting corncobs, sugar cane residue, wheat bran, rapeseed meal, zeolite powder, potassium dihydrogen phosphate, sodium chloride, light calcium carbonate, water and liquid strains in a fermentation bin. The preparation method of the microbial water-purifying agent comprises the following steps: firstly, crushing the corncobs into particles with diameters smaller than 5 mm; proportionally adding and mixing the sugar cane residue, the wheat bran, the rapeseed meal, the zeolite powder, the potassium dihydrogen phosphate, the sodium chloride and the light calcium carbonate; adding 25 percent of water; sending the mixture to an air steamer; adding high-pressure steam to the air steamer to increase the air pressure to 1-2 MPa and the temperature to 140-200 DEG C; keeping the pressure for 4-6 minutes; momentarily releasing the mixture in the air steamer; sending the mixture to the fermentation bin so that the temperature falls to 30-37 DEG C; then, inoculating 3-5 percent of strains; fermenting in the fermentation bin for 72 hours; and discharging from the bin to obtain a finished product. The product has good water-purifying effect, and air-steamed stalks are culture media for strain growth as well as excellent vectors. The invention can prepare strains by strains, stimulate the mass propagation of microorganisms to form dominant strains and have the yield-improving efficiency.

Description

A kind of microbial water-purifying agent and preparation method
Technical field
The present invention relates to the immobilized microorganism water purifying technology, adopting the quick-fried corn cob of vapour specifically is that main fixation support carries out multiple bacteria compound fermentation, produces the microbial water-purifying agent and the preparation method of aquaculture water and current water.
Background technology
In recent years, the high speed development of water industry makes the aquaculture water severe exacerbation, causes culturing disease and takes place in a large number, causes aquaculture water and contiguous body eutrophication when it is discharged in a large number; Though a large amount of on the other hand municipal effluents is handled by Sewage Plant, but the time have the sewage that exceeds standard to flow into river, lake, cause that eutrophication makes the algae and water overgrowing, hydrocoles such as fish are dead because of anoxic, brought huge pressure to environment, as Taihu Lake in the Dian Chi in Yunnan, Jiangsu arrived must improvement stage.Utilizing microorganism species is to utilize little ecological theory that water body is carried out biological restoration to polluted water purification, but how to bring into play flora in the polluted-water treating processes, effectively carries out biological the expansion and makes that to form high catalytic activity be the key that efficiently purifies water.And the key that forms such center makes microorganism species carry out cheapness, immobilization technology efficiently.
Trace it to its cause, fast development along with culture fishery, the scale of intensive industrialized aquaculture enlarges day by day, meanwhile, any discharging of undressed breeding wastewater, industry, sanitary sewage is polluted natural waters, the breeding ecological environmental degradation causes the disease problem of aquatic animal to be on the rise.The conditioning of aquatic products water quality is the water quality protection conditioning viable bacteria microbial preparation that is exclusively used in aquatic animal, can effectively suppress the breeding of aquatic pathogenic bacteria, strengthens the disease resistance of animal, safeguards the water ecology balance, reduces mortality ratio greatly.Adopt a series of high-tech means of microbial project more and obtain the high-performance bacterial strain in America and Japan Korea S man, form through Seiko is composite with other special effects materials again, include multiple prebiotic substances such as high-performance genus bacillus quasi-microorganism (living bacteria count at every gram more than 2,000,000,000), natural inner complex, to realize the microbial product of pure natural, green, can be described as antibiotic ideal substitute, can effectively improve the aquatic products yield and quality.In China's prior art, purification of water quality is adopted chemical materials or mineral mineral acid extractive technique more, as adopt Tai-Ace S 150 to add methods such as stimulator, sterilant, or adopt chlorinated lime to combine with alkali, alum, it can not realize quick degradation of organic substances and objectionable impurities, can not be by a large amount of breedings and the meta-bolites of functional microorganism in the product, the organism in the water body of fast and effeciently degrading decomposes multiple harmful substances such as ammonia-state nitrogen, nitrite, sulfide and phosphorus; Can not purify improvement water quality fully; Can not eliminate or significantly reduce the bottom and deposit by functional microorganism breeding and meta-bolites thereof in the product, purify improvement water quality, safeguard the water body environment eubiosis; Can not effectively suppress pathogenic bacteria, make in the product efficient high vigor functional microorganism breed the formation dominant microflora in a large number, the growth and breeding that has greatly suppressed pathogenic micro-organism with " bacterium " (probiotics energy bacterium) system " bacterium " (harmful bacterium such as pathogenic bacteria), strengthens the immunizing power of aquatic animal; Can not obviously increase economic efficiency.
Summary of the invention
Purpose of the present invention is intended to solve the deficiency of prior art problem, a kind of water quality cleansing agent is provided, ferment in fermentation cabin after vapour is quick-fried by screening different stalks, steam puffed stalk promptly is the flora substratum preferably of growing, and is again the excellent carrier that plays immobilization role; Can realize purifying water, make in the product efficient high vigor functional microorganism breed the formation dominant microflora in a large number, improve the effect of output with " bacterium " system " bacterium ".
The technical solution used in the present invention is: a kind of microbial water-purifying agent, adopt corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, light calcium carbonate, water and liquid spawn are made after the fermentation cabin fermentation, corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, the weight percent that light calcium carbonate and water add is: corn cob 50-70%, bagasse 5-20%, wheat bran 10-20%, dish dregs of rice 5-10%, zeolite powder 5-20%, potassium primary phosphate 0.15%, sodium-chlor 0.15%, light calcium carbonate 1%, water 15-30%, the add-on of liquid spawn is a corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, the 3-5% of light calcium carbonate and water total weight percent.
The present invention, a kind of preparation method of microbial water-purifying agent, step is carried out as follows:
(1), corn cob meal is broken to below the 5mm, make corn cob meal, standby;
(2), by weight percentage get corn cob meal 50-70%, bagasse 5-20%, wheat bran 10-20%, dish dregs of rice 5-10%, zeolite powder 5-20%, potassium primary phosphate 0.15%, sodium-chlor 0.15% and light calcium carbonate 1%, mix, after add the water of 15-30% by weight percentage, mix, make the moisture of material remain on 25%, make compound, standby;
(3), the compound that step (1) is made is sent into the quick-fried pot of vapour, the quick-fried pot door of sealing vapour;
(4), in the quick-fried pot of vapour, add high pressure steam, and the freezing air in the quick-fried pot of vapour is discharged, make the interior air pressure of the quick-fried pot of vapour rise to 1-2Mpa, temperature rises to 140-200 ℃, pressurize 4-6min;
(5), the material in the quick-fried pot of abrupt release vapour, and with vapour the material after quick-fried is sent into airtight fermentation cabin;
(6), after the material of vapour after quick-fried send in the fermentation cabin, adopt aseptic cold water formula plane heating panel and aseptic cold wind to dispel the heat simultaneously earlier, material temperature is reduced between 30-37 ℃, liquid spawn is inserted in the back, and the access amount is 3-5% by weight percentage;
(7), the compound that inserts bacterial classification was fermented in fermentation cabin 72 hours, temperature remains on 32-37 ℃; When the temperature of fermentation cabin surpasses 40 ℃, carry out anti-storehouse, or change material over to another fermentation cabin fermentation;
(8), finish 72 hours fermentation after, deliver from godown, pack makes finished product.
The particle diameter of each material described in the present invention is not more than 5mm.
A kind of microbial water-purifying agent of the present invention and preparation method, described liquid spawn, by subtilis, Bacillus licheniformis, fluorescent pseudomonas, actinomycetes, produce the protein yeast, whiterot fungi, Trichoderma, plant lactobacillus and photosynthetic bacteria are formed, the weight percent that each bacterial classification adds is: subtilis 10%-15%, Bacillus licheniformis 10%-15%, fluorescent pseudomonas 10%-15%, actinomycetes 10%-15%, produce protein yeast 10%-15%, whiterot fungi 10%-15%, Trichoderma 10%-15%, plant lactobacillus 10%-15%, photosynthetic bacteria 20%-30%.
A kind of microbial water-purifying agent of the present invention and preparation method, the substratum that described liquid spawn is used is: brown sugar 5-8%, dregs of beans 1-2%, potassium primary phosphate 0.2%, sodium-chlor 0.15%, light calcium carbonate 1%, surplus is a water, and initial p H value is transferred to 7.0.
The present invention when fermentation ends, can also add concentrated good nitrifier and anaerobic ammonia oxidizing bacteria 2%.
The bacterial classification that the present invention adopts all is known bacterial classification, can introduce by Chinese common micro-organisms culture presevation administrative center or the Chinese Academy of Agricultural Sciences microbial strains preservation center, is applied in the industrialization by screening.
Among the preparation method of the present invention, the cold water formula plane heating panel that adopts, its frame mode is: the bottom of heating panel is a concave shape groove, active cold water is arranged in the groove, groove face is provided with the flat panel that can hold material, when material is put in when dispelling the heat on the heating panel, dispel the heat with cold water in the bottom, dispels the heat with cold wind in top.
The invention has the beneficial effects as follows:
Adopting cheap steam puffed stalk is raw material, and carries out multiple bacteria compound fermentation in fermentation cabin, and the bacteria containing amount height of producing can be realized nitrated, denitrification, falls nitrogen, falls phosphorus, the water purification agent of complete function such as organic matter removal.Because the employing stalk is a main raw material, be again the good carrier of flora simultaneously simultaneously, in the process of purifying water, played the effect of active center.At cultivating pool, in the water purification process, can effectively suppress the harmful bacterium in the water body, reach preventing disease, improve the effect of surviving rate.Quicken the degraded of organic pollutant in the water body simultaneously, reduce ammonia nitrogen, improve dissolved oxygen content.Particularly this water purification agent purifies in the current water again, as: the sub-district septic tank is handled, fluidized bed processing, and this sewage purification to entire city acquires a special sense.
Adopt the method for gas-explosive material, can make the complete detoxification of material, handle by microbial fermentation again, its water quality cleansing agent of producing contains a large amount of probioticss energy bacterium, can realize purifying water, make in the product efficient high vigor functional microorganism breed the formation dominant microflora in a large number with " bacterium " system " bacterium ", theoretical breeding quantity can reach 272 in 24 hours, improved the effect of output; Reduce the mortality ratio more than 90%; Sexual valence when input-output ratio more than 1: 10; Can realize quick degradation of organic substances and objectionable impurities, by a large amount of breedings and the meta-bolites of functional microorganism in the product, the organism in the water body of fast and effeciently degrading decomposes multiple harmful substances such as ammonia-state nitrogen, nitrite, sulfide and phosphorus; Can purify improvement water quality fully; By functional microorganism breeding and meta-bolites thereof in the product, eliminate or significantly reduce the bottom and deposit, purify improvement water quality, safeguard the water body environment eubiosis; Disease-resistant function is strong, and scavenging agent itself is nontoxic, does not have problem of environment pollution caused, can suppress and overcome with cancer and subhealth state phenomenon be the increase of " food origin disease " of representative, reduces the problem that the food drug residue exceeds standard, the quality better of product, the quality height, cost is low, easily suitability for industrialized production.
Embodiment
A kind of microbial water-purifying agent, adopt corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, light calcium carbonate, water and liquid spawn are made after the fermentation cabin fermentation, corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, the weight percent that light calcium carbonate and water add is: corn cob 50-70%, bagasse 5-20%, wheat bran 10-20%, dish dregs of rice 5-10%, zeolite powder 5-20%, potassium primary phosphate 0.15%, sodium-chlor 0.15%, light calcium carbonate 1%, water 15-30%, the add-on of liquid spawn is a corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, the 3-5% of light calcium carbonate and water total weight percent.
The present invention, a kind of preparation method of microbial water-purifying agent, step is carried out as follows:
(1), corn cob meal is broken to below the 5mm, make corn cob meal, standby;
(2), by weight percentage get corn cob meal 50-70%, bagasse 5-20%, wheat bran 10-20%, dish dregs of rice 5-10%, zeolite powder 5-20%, potassium primary phosphate 0.15%, sodium-chlor 0.15% and light calcium carbonate 1%, mix, after add the water of 15-30% by weight percentage, mix, make the moisture of material remain on 25%, make compound, standby;
(3), the compound that step (1) is made is sent into the quick-fried pot of vapour, the quick-fried pot door of sealing vapour;
(4), in the quick-fried pot of vapour, add high pressure steam, and the freezing air in the quick-fried pot of vapour is discharged, make the interior air pressure of the quick-fried pot of vapour rise to 1-2Mpa, temperature rises to 140-200 ℃, pressurize 4-6min;
(5), the material in the quick-fried pot of abrupt release vapour, and with vapour the material after quick-fried is sent into airtight fermentation cabin;
(6), after the material of vapour after quick-fried send in the fermentation cabin, adopt aseptic cold water formula plane heating panel and aseptic cold wind to dispel the heat simultaneously earlier, material temperature is reduced between 30-37 ℃, liquid spawn is inserted in the back, and the access amount is 3-5% by weight percentage;
(7), the compound that inserts bacterial classification was fermented in fermentation cabin 72 hours, temperature remains on 32-37 ℃; When the temperature of fermentation cabin surpasses 40 ℃, carry out anti-storehouse, or change material over to another fermentation cabin fermentation;
(8), finish 72 hours fermentation after, deliver from godown, pack makes finished product.
The particle diameter of each material described in the present invention is not more than 5mm.
A kind of microbial water-purifying agent of the present invention and preparation method, described liquid spawn, by subtilis, Bacillus licheniformis, fluorescent pseudomonas, actinomycetes, produce the protein yeast, whiterot fungi, Trichoderma, plant lactobacillus and photosynthetic bacteria are formed, the weight percent that each bacterial classification adds is: subtilis 10%-15%, Bacillus licheniformis 10%-15%, fluorescent pseudomonas 10%-15%, actinomycetes 10%-15%, produce protein yeast 10%-15%, whiterot fungi 10%-15%, Trichoderma 10%-15%, plant lactobacillus 10%-15%, photosynthetic bacteria 20%-30%.
A kind of microbial water-purifying agent of the present invention and preparation method, the substratum that described liquid spawn is used is: brown sugar 5-8%, dregs of beans 1-2%, potassium primary phosphate 0.2%, sodium-chlor 0.15%, light calcium carbonate 1%, surplus is a water, and initial p H value is transferred to 7.0.
The present invention when fermentation ends, can also add concentrated good nitrifier and anaerobic ammonia oxidizing bacteria 2%.
Embodiment 1
Get corn cob 65%, bagasse 10%, wheat bran 10%, zeolite powder 8.7%, the dish dregs of rice 5%, potassium primary phosphate 0.15%, sodium-chlor 0.15%, light calcium carbonate 1% after mixing, adds the water of 15-30%, makes moisture reach 25%, and each treatment capacity is 10 tons.
Raw material after mixing is sent into the quick-fried pot of vapour, and in the quick-fried pot of vapour, feed high pressure steam, boost to 1.5Mpa behind the discharge freezing air, make the temperature in the quick-fried pot of vapour rise to 180 ℃, pressurize 6min.
Raw material in the quick-fried pot of abrupt release vapour, and send into the italic fermentation cabin of sealing; When temperature is reduced to 37 ℃, can connect the bacterial classification of mixed fermentation, inoculum size is 5%.
The 72h that in the italic fermentation cabin, ferments, temperature keeps 30-37 ℃, when temperature in the storehouse surpasses 40 ℃, can change another fermentation cabin over to, in whole fermentation process, roll-over 5 times.
In the roll-over the last time, add spissated nitrifier 1%, add anaerobic ammonia oxidizing bacteria 1% simultaneously.
Fermentation stops, and delivers from godown, and finished product is made in pack.
Embodiment 2
Get corn cob 55%, bagasse 20%, wheat bran 10%, zeolite powder 8.7%, the dish dregs of rice 5%, potassium primary phosphate 0.15%, sodium-chlor 0.15%, light calcium carbonate 1% after mixing, adds the water of 15-30%, makes moisture reach 25%, and each treatment capacity is 10 tons.
The raw material that mixes is sent into the quick-fried pot of vapour, and in the quick-fried pot of vapour, feed high pressure steam, boost to 1.8Mpa behind the discharge freezing air, make the temperature in the quick-fried pot of vapour rise to 200 ℃, pressurize 4min.
Raw material in the quick-fried pot of abrupt release vapour, and send into the italic fermentation cabin of sealing; When the fermentation cabin temperature of charge is reduced to 37 ℃, can insert the bacterial classification of mixed fermentation, inoculum size is 5%.
The 72h that in the italic fermentation cabin, ferments, temperature keeps 30-37 ℃, when temperature in the storehouse surpasses 40 ℃, can change another fermentation cabin over to, in whole fermentation process, roll-over 5 times.
In the roll-over the last time, add spissated nitrifier 1%, add anaerobic ammonia oxidizing bacteria 1% simultaneously.
Fermentation stops, and delivers from godown, and finished product is made in pack.
The result of use of trial employment period:
1, year August in April, 2007-2008, tries out in the fish pond of the Xinhua District Zhi of Pingdingshan City, Henan Province sun town western Zhi village raiser Kang Min, breed amount: 10 mu of fish ponds; The purification of water quality microbial inoculum that adopts my company to produce, the intensive culture grass carp, and cooperate the protein fodder of feeding our company's production, and output reaches 1750Kg/ mu, and than in other years, feed coefficient reduces by 18.2%, and net production improves 29.3%, and surviving rate reaches 92.5%.Its pond decontamination effect improving is: the comparison of aquaculture water dissolved oxygen is according to improving 16.2%, and ammonia nitrogen and nitrite have reduced by 54.4% and 68.5% respectively, and the transparency comparison improves 39.6 according to the pond, and biological oxygen demand (BOD) reduces by 34.7%, and Chi Shui is dark brown, based on brown alga.
2, year August in April, 2009-2009, Pingdingshan City's Xinhua District marine products bureau, in the experiment that Zhi sun town XiZhi Cun cultures loach, before not adding, ammonia nitrogen 1.8mg/L, dissolved oxygen 4mg/L, COD96mg/L; Measure ammonia nitrogen 1.4mg/L, dissolved oxygen 10.2mg/L, COD72mg/L after adding a week.Do not use preceding pond to cover with duckweed, add week back observation, duckweed all disappears in the water.
3, biotope septic tank experiment, before the use water purification agent, ammonia nitrogen 7.6mg/L, COD65mg/L; After the use, ammonia nitrogen 1.4mg/L, COD35mg/L have reached the sanitary sewage first discharge standard.

Claims (1)

1. the preparation method of a microbial water-purifying agent, by corn cob, bagasse, wheat bran, the dish dregs of rice, zeolite powder, potassium primary phosphate, sodium-chlor, light calcium carbonate, water and liquid spawn are made, corn cob, bagasse, wheat bran, the dish dregs of rice, zeolite powder, potassium primary phosphate, sodium-chlor, the weight percent that light calcium carbonate and water add is: corn cob 50-70%, bagasse 5-20%, wheat bran 10-20%, dish dregs of rice 5-10%, zeolite powder 5-20%, potassium primary phosphate 0.15%, sodium-chlor 0.15%, light calcium carbonate 1%, water 15-30%, described each material component sum is 100%; The add-on of liquid spawn is the 3-5% of corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, light calcium carbonate and water total weight percent;
Described liquid spawn is made up of subtilis, Bacillus licheniformis, fluorescent pseudomonas, actinomycetes, product protein yeast, whiterot fungi, Trichoderma, plant lactobacillus and photosynthetic bacteria, the weight percent that each bacterial classification adds is: subtilis 10-15%, Bacillus licheniformis 10-15%, fluorescent pseudomonas 10-15%, actinomycetes 10-15%, produce protein yeast 10-15%, whiterot fungi 10-15%, Trichoderma 10-15%, plant lactobacillus 10-15%, photosynthetic bacteria 20-30%, described each bacterial classification component sum is 100%;
Its preparation method carries out according to following steps:
(1), corn cob meal is broken to below the 5mm, make corn cob meal, standby;
(2), by weight percentage get corn cob meal 50-70%, bagasse 5-20%, wheat bran 10-20%, dish dregs of rice 5-10%, zeolite powder 5-20%, potassium primary phosphate 0.15%, sodium-chlor 0.15% and light calcium carbonate 1%, mix, after add the water of 15-30% by weight percentage, mix, make the moisture of material remain on 25%, make compound, standby;
(3), the compound that step (1) is made is sent into the quick-fried pot of vapour, the quick-fried pot door of sealing vapour;
(4), in the quick-fried pot of vapour, add high pressure steam, and the freezing air in the quick-fried pot of vapour is discharged, make the interior air pressure of the quick-fried pot of vapour rise to 1-2Mpa, temperature rises to 140-200 ℃, pressurize 4-6min;
(5), the material in the quick-fried pot of abrupt release vapour, and with vapour the material after quick-fried is sent into airtight fermentation cabin;
(6), after the material of vapour after quick-fried sent in the fermentation cabin, adopt aseptic cold water formula plane heating panel and aseptic cold wind to dispel the heat simultaneously earlier, material temperature is reduced between 30-37 ℃, liquid spawn is inserted in the back, and the add-on of liquid spawn is the 3-5% of corn cob, bagasse, wheat bran, rapeseed meal, zeolite powder, potassium primary phosphate, sodium-chlor, light calcium carbonate and water gross weight;
(7), the compound that inserts bacterial classification was fermented in fermentation cabin 72 hours, temperature remains on 32-37 ℃; When the temperature of fermentation cabin surpasses 40 ℃, carry out anti-storehouse, or change material over to another fermentation cabin fermentation;
(8), finish 72 hours fermentation after, deliver from godown, pack makes finished product.
CN2009100660227A 2009-09-02 2009-09-02 Microbial water-purifying agent and preparation method thereof Active CN101643280B (en)

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CN102689990B (en) * 2012-05-30 2013-05-08 中国海洋大学 Method for controlling water quality in shrimp pool by using bacillus and bagasse
CN103663873A (en) * 2013-12-09 2014-03-26 山东华亚环保科技有限公司 Water quality purification method
CN103787510B (en) * 2014-02-28 2015-01-21 中国水产科学研究院淡水渔业研究中心 Nutritional type filler for enriching indigenous microorganisms to purify water and preparation method thereof
CN104232510A (en) * 2014-08-06 2014-12-24 武汉禹轩环保科技有限公司 Microbial preparation capable of generating biological membrane in water body and using method of microbial preparation
CN104193013B (en) * 2014-09-16 2015-11-04 张苑 A kind of method utilizing bagasse to prepare improver of water quality
CN104828958A (en) * 2015-05-23 2015-08-12 张家港市山牧新材料技术开发有限公司 Microbial water purifying agent
CN105441352A (en) * 2015-09-16 2016-03-30 广州金水动物保健品有限公司 Compound microorganism water stabilizing preparation
CN107540097A (en) * 2017-07-20 2018-01-05 通化师范学院 A kind of microbial water-purifying agent for improving brachymystax lenok water quality and application thereof
CN111807524A (en) * 2020-07-01 2020-10-23 海南善水循环农业开发有限公司 Bottom material of red swamp crayfish

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