CN106418003A - Micro-ecological preparation combining water purification function for aquatic animals - Google Patents
Micro-ecological preparation combining water purification function for aquatic animals Download PDFInfo
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- CN106418003A CN106418003A CN201610888368.5A CN201610888368A CN106418003A CN 106418003 A CN106418003 A CN 106418003A CN 201610888368 A CN201610888368 A CN 201610888368A CN 106418003 A CN106418003 A CN 106418003A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Microbiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention discloses a micro-ecological preparation combining a water purification function for aquatic animals. The micro-ecological preparation is prepared by the following method: (1) plant compound extract preparing: according to the parts by weight: 2-5 parts of burdock roots, 2 parts of haws, 5 parts of radix astragali and 3 parts of fructus forsythia are weighed, 150 parts of deionized water is added, the materials are kept to be boiled for 50 min, then the boiled materials are put to be still for 24 hours, and then the put still materials are filtered using a filtering cloth at 200 meshes to obtain an extract and medicinal dregs; and 5 parts of the extract is taken to be mixed with 1 part of dreg removed mango peel juice to obtain a plant compound extract; and (2) preparing: bacillus subtilis, lactobacillus acidophilus, saccharomyces cerevisiae, corynebacterium glutamicum, acetobacter xylinum, the plant compound extract and beta-mannanase are evenly mixed to prepare the finished products of the micro-ecological preparation.
Description
Technical field
The present invention relates to a kind of aquatic animal microbial ecological agent for having water purification effect concurrently, belongs to aquaculture field.
Background technology
With a large amount of appearance of scale culture fishery, cultivation density is continuously increased, the Excreta in breeding water body with
And the residue of bait is continuously increased, pathogenic microorganism amount reproduction, breeding water body Eutrophic Extent aggravates, and ecological environment is suffered
Serious destruction, this causes aquatic animal extensive and frequently occurs all kinds of diseases and emergency rating, at the same time antibiotic and
The life-time service of antibacterial and abuse also further cause the residual of various medicines and pathogenic microorganism drug resistance in breeding water body
Property and drug resistance raising and, so as to destroy the ecological environment of breeding water body further.
And for important aquatic animal fish, intestinal is used as the immune organ of Fish itself maximum, intestinal health shape
Condition directly affects the digestion of nutrient substance and absorption, while the also immunity of impact fish body, premunition, intestinal health situation is in cultivation
In be increasingly taken seriously.Fish intestines health is kept it is necessary to have fresh, the interpolation medicine of the nutrition supply of abundance, guarantee raw material
The measures such as thing such as antibiotic, but as the phenomenon of medication lack of standardization is than more prominent, the residual problem of medicine becomes the focus of everybody concern.
Medicine should be reduced residual, promote animal intestinal health again, add microbial ecological agent and become one of current effective manner.Tiny ecosystem
Preparation can reduce drug residue, promote growth of animal, improves health conditions, safeguard ecological as the alternative of antibiotic
Balance, the research in cultivation with feedstuff is increasingly obtained paying attention to application and is strengthened.
Prior art typically reaches, by adding microbial ecological agent in feedstuff, the effect for promoting aquatic animal intestinal health
Really.The present invention is intended to provide a kind of aquatic animal microbial ecological agent for having water purification effect concurrently, which is only by splashing to water body
In can reach significant promotion aquatic health, the effect of purifying water body, easy to use, with low cost, with very big
Economic worth, has wide market prospect in aquatic products field.
Content of the invention
For the shortcoming of prior art, the present invention provides a kind of aquatic animal microbial ecological agent for having water purification effect concurrently,
Which can reach significant promotion aquatic health, the effect of purifying water body only by splashing in water body.
The purpose of the present invention is achieved through the following technical solutions：
A kind of aquatic animal microbial ecological agent for having water purification effect concurrently, which is prepared from by the following method：
(1) plant compound extracted solution is prepared：
According to weight portion, Radix Arctii 2-5 part is weighed, 2 parts of Fructus Crataegi, 5 parts of the Radix Astragali, 3 parts of Fructus Forsythiae, 150 parts of deionized waters are added,
50min is boiled in holding, then stands 24 hours, then the filter-cloth filtering with 200 mesh, obtains extracting solution and medicinal residues；
5 parts of extracting solution are taken, the 1 part of mangrove bark juice that removes slag mixes, and obtains plant compound extracted solution；
(2) prepare：
By bacillus subtilises, bacillus acidophilus, saccharomyces cerevisiae, Corynebacterium glutamicum, glue acetobacter, plant is combined
Extracting solution, 'beta '-mannase mix homogeneously, it is prepared into microbial ecological agent finished product；
Wherein, in microbial ecological agent finished product, the bacillus subtilises are 5 × 109Cfu/g, bacillus acidophilus be 1 ×
109Cfu/g, saccharomyces cerevisiae is 5 × 109Cfu/g, Corynebacterium glutamicum is 5 × 105Cfu/g, glue acetobacter be 2 ×
103cfu/g；
The enzyme activity of the 'beta '-mannase is 20000-25000IU/kg, and the consumption in finished product is 3g/kg；
Consumption of the plant compound extracted solution in finished product is 5-8g/kg.
The invention has benefit that：
1) this product effectively can be degraded water body residual bait, the Organic substance such as animal excrements, reduce organism oxygen consumption, promote water body
Beneficial organism and algae reproduction.
2) purify water, effectively remove the harmful substances such as ammonia nitrogen, cultured water.
3) optimize water body beneficial microbe fauna, suppression harmful microbe is bred, and makes beneficial microbe that advantage is occupied, living
Change breeding water body.
4) eliminate black, the abnormal water colour such as rubescent.
5) organic dirt such as decomposing pool bottom mud, residual bait and Excreta, reduces organic consumption.
6) functional oligose and immune polysaccharide are immunity of organism activator, can effectively improve interferon and original is phagocytal
Activity, excitating organism immunologic function, strengthen the immunity of watertight animal.
7) promote g and D, bait transformation efficiency is improved, reduce M ＆ M.
8) intestinal microbial population of Fish is adjusted.
Specific embodiment
Embodiment 1：
Embodiment of the present invention bacterial strain uses therefor be city available from.
A kind of aquatic animal microbial ecological agent for having water purification effect concurrently, which is prepared from by the following method：
(1) plant compound extracted solution is prepared：
According to weight portion, 2 parts of Radix Arctii, 2 parts of Fructus Crataegi, 5 parts of the Radix Astragali, 3 parts of Fructus Forsythiae, 150 parts of deionized waters of addition, guarantor is weighed
Hold and 50min is boiled, then 24 hours are stood, then the filter-cloth filtering with 200 mesh, obtain extracting solution and medicinal residues；
5 parts of extracting solution are taken, the 1 part of mangrove bark juice that removes slag mixes, and obtains plant compound extracted solution；
(2) prepare：
By bacillus subtilises, bacillus acidophilus, saccharomyces cerevisiae, Corynebacterium glutamicum, glue acetobacter, plant is combined
Extracting solution, 'beta '-mannase mix homogeneously, it is prepared into microbial ecological agent finished product；
Wherein, in microbial ecological agent finished product, the bacillus subtilises are 5 × 109Cfu/g, bacillus acidophilus be 1 ×
109Cfu/g, saccharomyces cerevisiae is 5 × 109Cfu/g, Corynebacterium glutamicum is 5 × 105Cfu/g, glue acetobacter be 2 ×
103cfu/g；
The enzyme activity of the 'beta '-mannase is 25000IU/kg, and the consumption in finished product is 3g/kg；
Consumption of the plant compound extracted solution in finished product is 5g/kg.
Embodiment 2：
A kind of aquatic animal microbial ecological agent for having water purification effect concurrently, which is prepared from by the following method：
(1) plant compound extracted solution is prepared：
According to weight portion, 5 parts of Radix Arctii, 2 parts of Fructus Crataegi, 5 parts of the Radix Astragali, 3 parts of Fructus Forsythiae, 150 parts of deionized waters of addition, guarantor is weighed
Hold and 50min is boiled, then 24 hours are stood, then the filter-cloth filtering with 200 mesh, obtain extracting solution and medicinal residues；
5 parts of extracting solution are taken, the 1 part of mangrove bark juice that removes slag mixes, and obtains plant compound extracted solution；
(2) prepare：
By bacillus subtilises, bacillus acidophilus, saccharomyces cerevisiae, Corynebacterium glutamicum, glue acetobacter, plant is combined
Extracting solution, 'beta '-mannase mix homogeneously, it is prepared into microbial ecological agent finished product；
Wherein, in microbial ecological agent finished product, the bacillus subtilises are 5 × 109Cfu/g, bacillus acidophilus be 1 ×
109Cfu/g, saccharomyces cerevisiae is 5 × 109Cfu/g, Corynebacterium glutamicum is 5 × 105Cfu/g, glue acetobacter be 2 ×
103cfu/g；
The enzyme activity of the 'beta '-mannase is 20000IU/kg, and the consumption in finished product is 3g/kg；
Consumption of the plant compound extracted solution in finished product is 8g/kg.
Embodiment 3：
A kind of aquatic animal microbial ecological agent for having water purification effect concurrently, which is prepared from by the following method：
(1) plant compound extracted solution is prepared：
According to weight portion, 3 parts of Radix Arctii, 2 parts of Fructus Crataegi, 5 parts of the Radix Astragali, 3 parts of Fructus Forsythiae, 150 parts of deionized waters of addition, guarantor is weighed
Hold and 50min is boiled, then 24 hours are stood, then the filter-cloth filtering with 200 mesh, obtain extracting solution and medicinal residues；
5 parts of extracting solution are taken, the 1 part of mangrove bark juice that removes slag mixes, and obtains plant compound extracted solution；
(2) prepare：
By bacillus subtilises, bacillus acidophilus, saccharomyces cerevisiae, Corynebacterium glutamicum, glue acetobacter, plant is combined
Extracting solution, 'beta '-mannase mix homogeneously, it is prepared into microbial ecological agent finished product；
Wherein, in microbial ecological agent finished product, the bacillus subtilises are 5 × 109Cfu/g, bacillus acidophilus be 1 ×
109Cfu/g, saccharomyces cerevisiae is 5 × 109Cfu/g, Corynebacterium glutamicum is 5 × 105Cfu/g, glue acetobacter be 2 ×
103cfu/g；
The enzyme activity of the 'beta '-mannase is 24000IU/kg, and the consumption in finished product is 3g/kg；
Consumption of the plant compound extracted solution in finished product is 6g/kg.
Embodiment 4：
A kind of aquatic animal microbial ecological agent for having water purification effect concurrently, which is prepared from by the following method：
(1) plant compound extracted solution is prepared：
According to weight portion, 4 parts of Radix Arctii, 2 parts of Fructus Crataegi, 5 parts of the Radix Astragali, 3 parts of Fructus Forsythiae, 150 parts of deionized waters of addition, guarantor is weighed
Hold and 50min is boiled, then 24 hours are stood, then the filter-cloth filtering with 200 mesh, obtain extracting solution and medicinal residues；
5 parts of extracting solution are taken, the 1 part of mangrove bark juice that removes slag mixes, and obtains plant compound extracted solution；
(2) prepare：
By bacillus subtilises, bacillus acidophilus, saccharomyces cerevisiae, Corynebacterium glutamicum, glue acetobacter, plant is combined
Extracting solution, 'beta '-mannase mix homogeneously, it is prepared into microbial ecological agent finished product；
Wherein, in microbial ecological agent finished product, the bacillus subtilises are 5 × 109Cfu/g, bacillus acidophilus be 1 ×
109Cfu/g, saccharomyces cerevisiae is 5 × 109Cfu/g, Corynebacterium glutamicum is 5 × 105Cfu/g, glue acetobacter be 2 ×
103cfu/g；
The enzyme activity of the 'beta '-mannase is 21000IU/kg, and the consumption in finished product is 3g/kg；
Consumption of the plant compound extracted solution in finished product is 7g/kg.
Embodiment 5：
Contrast test is added without Corynebacterium glutamicum, glue acetobacter, plant compound extracted solution and 'beta '-mannase：
A kind of aquatic animal microbial ecological agent for having water purification effect concurrently, which is prepared from by the following method：
Prepare：
By bacillus subtilises, bacillus acidophilus, saccharomyces cerevisiae mix homogeneously makes finished product；
Wherein, in microbial ecological agent finished product, the bacillus subtilises are 5 × 109Cfu/g, bacillus acidophilus be 1 ×
109Cfu/g, saccharomyces cerevisiae is 5 × 109cfu/g；
6 result of embodiment is tested and assessed：
Usage and dosage：
Per kilogram is used for 1000-1500 square metre of (2-3 mu) pond (1 meter of the depth of water).20 days using once.Dilute with pond water
Splash after releasing.
Fine day noon used, using front opening aerator more than 30 minutes.
(1) fishpond effect test and appraisal：
Test fishpond：The Cyprinus carpiovar Jian then that test is selected, initial average weight is (18.25 ± 0.11) g, physical health, will
Fingerling is randomly divided into 6 groups, 4 groups of fishpond of experiment, and 2 groups of matched group is repeated 3 times per group.
Matched group 1 is the microbial ecological agent of embodiment 5 of splashing, and matched group 2 is blank control group, without any Tiny ecosystem
Preparation.
Fishpond area is 8 mu, puts Cyprinus carpio in a suitable place to breed for per mu and amounts to about 90kg.
All fries are raised according to conventional daily ration, and method for breeding is identical, and experimental period is 40 days.
Impact result to water quality is as shown in the table.
Table 1
| Group | Water body COD value at the end of experiment | Ammonia-nitrogen content (mg/mL) |
| Embodiment 1 | 3.4 | 0.02 |
| Embodiment 2 | 4.7 | 0.03 |
| Embodiment 3 | 3.3 | 0.01 |
| Embodiment 4 | 5.1 | 0.04 |
| Matched group 1 | 9.5 | 0.3 |
| Matched group 2 | 16.8 | 0.6 |
Result of the test shows：The microbial ecological agent of the present invention can significantly improve water purity, reduce COD, reduce
Ammonia nitrogen.Compare with matched group 1, effect also improves a lot, between this explanation Multiple components, serve certain synergism.
In addition, it was further observed that the microbial ecological agent of the present invention have eliminate black, the effect of the abnormal water colour such as rubescent.
(2) fish intestinal bacteria analysis：
Contain substantial amounts of antibacterial in water body, decisive action is played to the composition of early stage intestinal microbial population, the antibacterial group in water body
Bacterial flora, especially the ancestral home flora for becoming and being colonized in fish intestines, has the contact that certain is inevitable.Animal intestinal autochthonous flora
In, anaerobe accounts for more than 90%, and antibacterial has obvious antagonism to external world.Bacterial species under normal condition, in fish intestinal
With quantity in dynamic balance.Ecological deterioration, catch, density is excessive etc., and factor can destroy this balance, make cause of disease
Bacterium or conditioned pathogen abnormality proliferation, show pathological state so as to cultivate object, and growth performance declines.
Intestinal microbial population is determined：
After growth test terminates, each treatment group takes 3 tail fishes (being satiated with food after feeding 2h) at random, with 75% alcohol disinfecting fish body
Table, opens abdominal cavity sterilization intestinal tube outer wall, and sterile cotton knot is pricked, with aseptic water washing, intestinal chyme in taking out under aseptic condition.Surveyed
Microflora is represented with lgcfu/g.
Impact result to Cyprinus carpio intestinal bacteria is as shown in the table.
Table 2
| Group | Total bacteria count | Vibrio number | Lactic acid bacteria number | Coliform count | Mortality rate (%) |
| Embodiment 1 | 7.38 | 3.26 | 7.65 | 5.55 | 0.5 |
| Embodiment 2 | 7.28 | 6.31 | 6.34 | 5.75 | 1.8 |
| Embodiment 3 | 7.36 | 6.43 | 6.25 | 5.43 | 2.4 |
| Embodiment 4 | 7.30 | 6.23 | 6.40 | 5.88 | 1.6 |
| Matched group 1 | 6.81 | 6.98 | 6.32 | 6.21 | 2.8 |
| Matched group 2 | 6.65 | 7.26 | 6.21 | 6.12 | 6.3 |
As can be seen here, this is only by splashing, it is not necessary to mix feedstuff, the microbial ecological agent of invention can just be significantly inhibited
Escherichia coli are had relatively by pathogenic vibrio (especially the effect of embodiment 1 is compared with significant difference with other experimental grouies)
Think inhibition well, then have obvious facilitation effect to lactic acid bacteria.Promote g and D, bait transformation efficiency is improved, reduce
M ＆ M.
To sum up, this product effectively can be degraded water body residual bait, the Organic substance such as animal excrements, reduce organism oxygen consumption, promote
Water body beneficial organism and algae reproduction.Can purify water, effectively remove the harmful substances such as ammonia nitrogen, cultured water.Can optimize
Water body beneficial microbe fauna, suppression harmful microbe breeding, make beneficial microbe that advantage is occupied, activate breeding water body.Can
With eliminate black, the abnormal water colour such as rubescent.Organic consumption can be reduced with the organic dirt such as decomposing pool bottom mud, residual bait and Excreta
Amount.The functional oligose of this product and immune polysaccharide are immunity of organism activator, can effectively improve interferon and former phagocyte
Activity, excitating organism immunologic function, strengthen watertight animal immunity.G and D can be promoted, improve bait conversion
Efficiency, reduces M ＆ M.The intestinal microbial population of Fish can be adjusted.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any those familiar with the art the invention discloses technical scope in, technology according to the present invention scheme and its
Inventive concept equivalent or change in addition, should all be included within the scope of the present invention.
Claims (6)
1. a kind of have concurrently water purification effect aquatic animal microbial ecological agent, which is prepared from by the following method：
(1) plant compound extracted solution is prepared：
According to weight portion, Radix Arctii 2-5 part, 2 parts of Fructus Crataegi, 5 parts of the Radix Astragali, 3 parts of Fructus Forsythiae, 150 parts of deionized waters of addition, holding is weighed
50min is boiled, then 24 hours is stood, then the filter-cloth filtering with 200 mesh, obtain extracting solution and medicinal residues；
5 parts of extracting solution are taken, the 1 part of mangrove bark juice that removes slag mixes, and obtains plant compound extracted solution；
(2) prepare：
By bacillus subtilises, bacillus acidophilus, saccharomyces cerevisiae, Corynebacterium glutamicum, glue acetobacter, plant compound collecting
Liquid, 'beta '-mannase mix homogeneously, it is prepared into microbial ecological agent finished product.
2. described in claim 1 have concurrently water purification effect aquatic animal microbial ecological agent, it is characterised in that：
In microbial ecological agent finished product, the bacillus subtilises are 5 × 109Cfu/g, bacillus acidophilus are 1 × 109Cfu/g,
Saccharomyces cerevisiae is 5 × 109Cfu/g, Corynebacterium glutamicum is 5 × 105Cfu/g, glue acetobacter is 2 × 103cfu/g.
3. described in claim 1 have concurrently water purification effect aquatic animal microbial ecological agent, it is characterised in that：
The enzyme activity of the 'beta '-mannase is 20000-25000IU/kg, and the consumption in finished product is 3g/kg.
4. described in claim 1 have concurrently water purification effect aquatic animal microbial ecological agent, it is characterised in that：
Consumption of the plant compound extracted solution in finished product is 5-8g/kg.
5. application of the aquatic animal microbial ecological agent for having water purification effect concurrently described in claim 1-4 in aquaculture.
6. the application described in claim 5, it is characterised in that：
Usage and dosage：
Per kilogram is used for 1000-1500 square metre of (2-3 mu) pond (1 meter of the depth of water).20 days using once.After pond water dilution
Splash；
Fine day noon used, using front opening aerator more than 30 minutes.
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| CN201610888368.5A CN106418003B (en) | 2016-10-11 | 2016-10-11 | A kind of aquatic livestock probiotics having both water purification effect |
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| CN201610888368.5A CN106418003B (en) | 2016-10-11 | 2016-10-11 | A kind of aquatic livestock probiotics having both water purification effect |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107691295A (en) * | 2017-09-18 | 2018-02-16 | 开县泰旭农业综合开发有限公司 | A kind of cultural method for improving white snakeheaded fish yield and quality |
| CN112156175A (en) * | 2020-11-09 | 2021-01-01 | 柳州市绿威生物科技有限公司 | Composition for degrading drug residues in live fish and preparation method thereof |
| CN113716705A (en) * | 2021-09-01 | 2021-11-30 | 英丽(中山)生物科技有限公司 | Natural plant extract composition capable of improving microbial degradation capability and application thereof |
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| CN107691295A (en) * | 2017-09-18 | 2018-02-16 | 开县泰旭农业综合开发有限公司 | A kind of cultural method for improving white snakeheaded fish yield and quality |
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| CN113716705A (en) * | 2021-09-01 | 2021-11-30 | 英丽(中山)生物科技有限公司 | Natural plant extract composition capable of improving microbial degradation capability and application thereof |
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| CN106418003B (en) | 2019-07-02 |
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Address after: 201612 Shanghai Songjiang District Songjiang Industrial Park 1505 Applicant after: Shanghai dragon Biotechnology Group Co., Ltd. Address before: 201612 Shanghai Songjiang District Songjiang Industrial Park 1505 Applicant before: Shanghai Gaolong Biotechnology Co., Ltd. |
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