CN103891674A - Ecological cultivation method with health care fermented feed matched with probiotic fermentation bed - Google Patents

Ecological cultivation method with health care fermented feed matched with probiotic fermentation bed Download PDF

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CN103891674A
CN103891674A CN 201410155490 CN201410155490A CN103891674A CN 103891674 A CN103891674 A CN 103891674A CN 201410155490 CN201410155490 CN 201410155490 CN 201410155490 A CN201410155490 A CN 201410155490A CN 103891674 A CN103891674 A CN 103891674A
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fermentation
probiotic
bed
method
feed
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CN 201410155490
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Chinese (zh)
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石建华
刘全
于纪国
王春华
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北京华牧伟业科技有限公司
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing
    • Y02P60/87Re-use of by-products of food processing for fodder production
    • Y02P60/877Re-use of by-products of food processing for fodder production from by-products of vegetal origin
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
    • Y02W30/43Aerobic fermentation, e.g. composting
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
    • Y02W30/47Anaerobic fermentation, e.g. methanation combined with capture, recycling or flaring

Abstract

The invention relates to an ecological cultivation method with health care fermented feed matched with a probiotic fermentation bed. The method includes the steps that first, probiotic fermentation is carried out, wherein the probiotic fermentation comprises anaerobic fermentation of anaerobes and aerobic fermentation of aerobic microorganisms; second, the probiotic fermentation bed is prepared, wherein various raw materials for preparing the material of the fermentation bed are evenly mixed so as to obtain organic bedding, bacterium solution is inoculated for fermentation, and the organic bedding contains saw dust, rice husks, bran and first compound Chinese herbal medicine; third, the fermented feed contains corn, bean pulp, vitamins, microelements, mineral substances, amino acids, other additives, second compound Chinese herbal medicine and the like, and microorganisms are added into the feed components for fermentation. The ecological cultivation method has the advantages that water can be purified, and hidden danger to health of hogs caused by a water source is blocked; the using amount of the microelements is reduced, resources are saved, emissions are reduced, and the environment is protected; no antibiotics or residue exists, and food safety and human health are guaranteed; the Chinese herbal medicines have the functions of being resistant to disease and promoting growth and can replace antibiotics.

Description

一种保健发酵饲料配合益生菌发酵床的生态养殖方法 A health fermented feed with ecological farming methods probiotic fermentation bed

技术领域 FIELD

[0001 ] 本发明涉农业领域中生物发酵技术应用到微量元素和中草药领域,并在饲料行业中得以应用,具体涉及一种保健发酵饲料配合益生菌发酵床的生态养殖方法。 [0001] The present invention relates to fermentation technology to the field of agriculture and trace elements in the field of herbal medicine, and be applied in the feed industry, particularly relates to a method of ecological farming with probiotic fermented fermented feed care bed.

背景技术 Background technique

[0002] 近年来随着养殖业规模化、商业化的发展,生产者一直致力于动物生产性能和数量的提高,但在动物性食品安全和环境污染问题上并未引起足够的重视。 [0002] In recent years, with the development of aquaculture scale, commercial producers have been dedicated to the production performance and increase the number of animals, but did not attract enough attention on animal food safety and environmental pollution problems. 以致食品安全和环境污染事件不断发生。 So that food safety and environmental pollution incidents continue to occur. 虽已倍受国家重视,但在利益因素的驱使下,并没有得到有效改善。 Although much national attention, but driven by the interests of factors, not been effectively improved. 因此,以致越来越多的人们渴望国家能加强对食品安全和环境染污的监控,保证人们能够吃到安全放心健康的食品,目前国家已制定一系列法律法规,但这都不能起到有效遏制的作用。 Therefore, so that more and more people want countries to strengthen the monitoring of food safety and environmental soiled, to ensure safe and secure people can eat healthy food, the country has formulated a series of laws and regulations, but can not play an effective containment role. 目前我国集约化养殖造成的环境污染和畜产品残留问题比较严重。 Currently environmental pollution and animal residues of intensive farming caused by more serious. 主要是饲料中添补的微量元素大多为无机盐形式,由于动物机体对无机态的矿物元素消化吸收能力差,利用率低,所以必须超量添加才能起到应有的作用。 The main feed is mostly fill trace elements in the form of salts, since the body of the animal on inorganic mineral elements of poor digestion and absorption, low utilization rate, it is necessary to add excess in order to play its role. 这是其一,其二是养猪生产中普遍采用闻铜(硫酸铜)、闻锋(氧化锋)和闻抗生素日粮,以提闻动物的生广性能。 This is one, the second is commonly used in the production of pig smell copper (copper sulfate), Wen Feng (oxidation front) and dietary antibiotic smell, the smell of animals to provide a wide raw performance. 在造成资料浪费、环境污染的同时,影响畜产品的质量和人类健康。 At the same time resulting in a waste of materials, environmental pollution, affecting the quality of livestock and human health.

[0003] Barber等(1995)首次发现在饲料中添加动物正常需要量10倍的铜,可明显改进其生长速度和饲料转化。 [0003] Barber et al. (1995) found that addition of the first 10 times the normal animal required amount of copper in the feed, can be significantly improved growth rate and feed conversion. 人们还发现高剂量的氧化锌能够提高仔猪的日采食量和日增重降低料肉比和仔猪腹泻率,饲料生产企业和养殖户在乳猪饲料中普遍添加2000-3000mg/kg甚至更高的氧化锌高剂量微量元素(高铜和高锌)作为有效的促生长添加剂,已成为养猪业较为广泛的营养调控手段。 It has also been found that high doses of zinc oxide can improve piglet feed intake and daily gain and lower feed conversion rates of diarrhea, feed manufacturers and farmers generally add 2000-3000mg / kg or higher in suckling pig feed trace elements zinc oxide high doses (high copper and high zinc) as an effective growth-promoting additive, the pork industry has become more widely nutritional control means.

[0004] 在养猪生产中,高铜作为促生长添加剂使用相当普遍,由于微量元素间的拮抗作用,同时也提高了锌、铁等微量元素的用量。 [0004] In the pig production, high copper used as a growth-promoting additive is quite common, because antagonism between the trace elements, but also improve the amounts of zinc, iron and other trace elements. 猪饲料中使用大量的铜(Cu)、锌(Zn)、铁(Fe)等无机微量元素由于吸收利用率低,微量元素经粪便大量排出,对环境(土壤、水系等)造成严重污染。 Pig feed a large amount of copper (Cu), zinc (Zn), iron (Fe) and other trace elements in the low utilization rate of absorption, a large number of trace elements faeces discharged to the environment (soil, water, etc.) cause serious pollution. 鉴于高剂量微量元素添加造成的危害,欧共体已提出了饲料中铜允许量的卫生标准。 Given the high dose of trace elements add the harm caused, the EC has proposed to allow the amount of copper in the feed hygiene standards. 规定16周内小猪175mg/kg, 17周以上为35mg/kg,加拿大、美国饲料协会已将日粮中铜和锌最大限量分别规定为125mg/kg和500mg/kg。 16 weeks piglets predetermined 175mg / kg, more than 17 weeks of 35mg / kg, Canada, American Feed Association dietary copper and zinc have maximum limits are defined as 125mg / kg and 500mg / kg. 我国也对饲料中微量元素使用量做出调整,小猪200mg/kg,育肥猪为35mg/kg。 China has also made adjustments to the amount of trace elements in the feed, pig 200mg / kg, fattening pigs is 35mg / kg. 高铜不但可以引起猪只铜中毒,还引起猪的某些营养素(如VA、VD、VE、VB2)缺乏,当饲料中铜含量高达250ppm时,对猪肉品质有不良的影,从而导致食品安全问题。 Not only can cause high copper copper poisoning pigs, pigs also cause certain nutrients (such as VA, VD, VE, VB2) deficiency, when the feed copper content of up to 250ppm, a bad shadow on pork quality, leading to food safety problem. 荷兰考虑到环境保护,不再允许在日粮中使用高铜和高锌作为促生长剂:日本也对铜、锌的用量进行严格限制。 Netherlands consideration of environmental protection, no longer allowed to use high copper and high zinc in the diet as a growth promoter: Japan also, the amount of zinc copper strictly restricted.

[0005] 在现代畜牧业生产对环境的污染中,铜、锌等微量元素占绝大部分。 [0005] In modern livestock production pollution of the environment, the copper, zinc and other trace elements account for most. 这不仅导致动物微量元素营养过剩和经济上的浪费,而且对生态环境造成严重污染。 This not only leads to excess nutrients and trace elements in animal waste on the economy, but also caused serious pollution to the environment. 据报道,我国每年使用的微量元素添加剂为15~18万吨,但由于其利用率低,大约有10万吨左右未被动物利用的矿物质随粪尿排出污染环境成为一大公害。 According to reports, trace elements additives used in China each year 15 to 18 million tons, but because of its low utilization rate of about 10 million tons of minerals have not been utilized with animal manure discharge pollution has become a major nuisance. 一般饲料中无机微量元素如铜的添加量是机体正常需要量的20~40倍,饲料中铜代谢后90%经粪便排,铜为不可降解物质,必然会造成土壤、水源、植被严重污,阻碍环保型农业的发展。 Feeds with inorganic trace elements such as copper is added in an amount 20 to 40 times the required amount of the body's normal, 90% of the feed by the fecal copper metabolism, a copper material is non-degradable, will definitely lead to soil, water, vegetation serious pollution, hinder the development of environment-friendly agriculture. 锌大部分是来自日粮中未被吸收的外源锌,研究表明饲喂高剂量锌日粮组猪每日锌排出量是饲喂基础日粮组的33倍,如此高剂量的锌经粪便排泄入环境中,使土壤锌积累而污染环境。 Zinc and zinc mostly not absorbed from the diet, feeding studies have shown that diets high dosage zinc zinc pigs daily discharge amount is 33 times the basal diet group, such high doses of zinc fecal excreted into the environment, the soil and accumulate zinc pollution of the environment. 据日本有关专家推测如果所有猪粪中的锌停留在土壤中,锌含量将在17年内超标120mg/kg被污染的土地将不再具有可耕性,锌对鱼类和水生生物的毒性更大渔业水质要求锌在0.lmg/L之内过量的锌经畜体排泄后还会污染地下水妨碍畜牧业的可持续发展 According to Japanese experts speculate more zinc toxicity to fish and aquatic organisms if all the zinc pig manure stays in the soil, the zinc content exceeding 17 years in the 120mg / kg of contaminated land will no longer arable, fisheries quality requirements in excess zinc 0.lmg / L of zinc was excreted after carcass contamination of groundwater will interfere sustainable development of animal husbandry

[0006]如此高剂量的微量元素用量,势必会影响畜产品品质,我国肉类食品中铜限量卫生标准(GB15199-94)规定,猪肉中铜含量不超过lOppm。 [0006] The amount of such a high dose of trace elements, is bound to affect the quality of livestock products, our meat products in copper tolerance limit (GB15199-94) provides that pork copper content does not exceed lOppm. 高铜日粮可导致猪肝铜含量增加10-15倍,人食入含铜高的畜产品后可造成铜在肝、脑及肾等组织中累积,增加机体内自由基的水平,改变脂类代谢,导致动脉粥样硬化并加速细胞的老化和死亡。 Pig diets can lead to high copper content increases 10-15 fold copper, copper can cause liver, kidney brain and other tissues accumulate, increasing the level of free radicals in the body after ingestion of a high copper-containing human and livestock, lipid changes class metabolism, leading to atherosclerosis and accelerated cell aging and death.

[0007]自本世纪50年代,动物科学家们发现,饲料中低浓度的抗生素可明显地促进畜禽生长,饲料抗生素就广为使用,世界上各饲料生产国几乎无一不在饲料中添加各种抗生素。 [0007] Since the 1950s, scientists found the animals, feed low concentrations of antibiotics can significantly promote animal growth, feed on antibiotics widely used, feed each producing country in the world almost without exception, add a variety of in feed antibiotic. 应该说近半个世纪以来,饲料抗生素对预防动物疾病,促进动物生长,提高饲料报酬确实起到了积极的作用。 It should be said that nearly half a century, animal feed antibiotics to prevent animal diseases, to promote animal growth, improve feed does play a positive role. 据统计世界上有二十多种抗生素及十几种合成抗菌类药物被应用到饲料中。 According to statistics in the world have two dozen dozen antibiotics and synthetic antibacterial drugs are applied to the feed. 60年代至80年代初是抗生素被饲料行业应用得最多的时期。 In the early 1960s to the 1980s is the most antibiotics are applied during the feed industry.

[0008] 众所周知,抗生素作为生产促进剂对畜牧业的发展做出了巨大贡献。 [0008] It is well known antibiotics as production enhancers on the development of animal husbandry has made tremendous contributions. 然而,抗生素的不规范使用所引起的药物残留,耐药性等问题已经引起高度重视。 However, the drug does not regulate the use of antibiotics caused by residual drug resistance and other problems have attracted attention. 多年来,由于我国养殖户长期大量使用抗生素,使畜禽有益菌群被杀灭,导致机体菌群失调、二重感染、耐药性等,畜禽免疫功能与抗病力、生产性能下降。 Over the years, due to the large number of farmers long-term use of antibiotics, the beneficial bacteria are killed livestock, causing the body flora, superinfection, drug resistance, immune function and disease resistance of livestock and poultry production performance. 养殖成本增加。 Increase in farming costs. 不仅如此,在过去多年的时间里,欧洲和美国的专家就此进行了调查,结论是细菌在动物体内对药物的耐受性可能转移给人。 Moreover, in the past years time, European and American experts conducted an investigation and concluded that bacteria in animals tolerated the drug may be transferred to humans. 耐药性甚至可通过畜禽产品传递给人体,给人类的健康、治疗带来威胁。 Even resistance can be passed to humans through animal products, to human health, the treatment of a threat. 据中国兽医杂志报道(1992),近几年令人惶恐的“疯牛病”也是由于长期给牛注射抗生素、激素和羊马下水制成的一种三合一针剂导致,又如“伪膜肠,又称抗生素肠炎”的发生也与滥用抗生素有密切关系。 According to Chinese Journal of Veterinary Medicine report (1992), in recent years, it is fear of "mad cow disease" is also due to the long cattle antibiotics, hormones and one triple injection Yangma made of lead into the water, and as "pseudomembranous intestine, also known as antibiotic enteritis "occurs also closely related to the abuse of antibiotics.

[0009] 抗生素的使用不仅抑制或杀灭了病原微生物,同时也抑制或杀灭了动物机体内的有益菌,打破了微生态平衡,导致动物某些维生素缺乏,免疫能力降低,甚至使非敏感菌群和真菌大量繁殖,引致消化吸收的障碍以至继发性感染,造成动物发病或死亡。 [0009] The use of antibiotics not only inhibit or kill the pathogenic microorganisms, but also inhibit or kill the animal body's beneficial bacteria, breaking the micro-ecological balance, resulting in some animal vitamin deficiency, reduced immunity, so that even non-sensitive flora and fungi multiply, causing disorders of digestion and absorption as well as secondary infection, resulting in animal morbidity or mortality. 如长期使用抗生素,使畜禽机体产生依赖性,限制了体内免疫细胞机能的发挥,使机体免疫功能下降,抵抗疾病能力降低。 The long-term use of antibiotics, make the body produce dependence livestock, the immune cell function limits play the decreased immune function, reduced ability to resist disease. 抗生素随饲料在动物消化道发挥作用后,进入动物的血液循环,经肾脏的过滤大多数随尿液排出体外,但仍然有一些残留在动物体内。 After antibiotics with animal feed in the digestive tract play a role, into the animal's blood circulation, most with urine excreted by the kidney filtration, but there are still some left in the animals. 残留的这些抗生素积累在动物产品如肉、蛋、奶、毛中,直接威胁着人体健康。 These antibiotic residues accumulate in animal products such as meat, eggs, milk, Mauritius, a direct threat to human health. 使用抗生素有可能使动物产生基因突变、畸形、致癌;抗生素大量排出动物体外,也可能造成环境污染。 Use of antibiotics has the potential to produce an animal gene mutation, deformity, carcinogenic; animal body discharge of antibiotics, may also cause environmental pollution. 据有关报道显示,在饲料中长时间的使用低剂量的抗生素,会增加耐药性产生的几率。 According to the reports indicate that the use of low-dose antibiotics for a long time in the feed, increases the risk of drug resistance.

[0010] 鉴于抗生素严重的副作用,欧盟委员会立法从1999年7月I日起禁止在饲料中添加杆菌肽、螺旋霉素、维吉尼亚霉素、泰乐菌素4种抗生素。 [0010] In view of the serious side effects of antibiotics, the European Commission legislation since July 1999, I added the date is prohibited in feed bacitracin, spiramycin, virginiamycin, tylosin four antibiotics. 并规定在动物的组织中必须不能检出有药物残留,这就对我国出口畜禽产品提出了更高的要求。 And specified in the tissue of an animal must not have detectable drug residues, of which China's exports of livestock and poultry products has put forward higher requirements. 目前,美国FDA已从农业用途中取消了青霉素、四环素、红霉素、林可霉素、泰乐菌素和维吉尼亚霉素。 Currently, the FDA removed from agricultural use of penicillin, tetracycline, erythromycin, clindamycin, virginiamycin and tylosin.

[0011] 为了解决畜产品安全性隐患等问题,各国学者开展了对绿色饲料添加剂的研究。 [0011] In order to address the security risks and other issues of animal products, scholars from various countries carried out research on green feed additives. 现已有微生态制剂和酶制剂,微生态制剂是通过增加消化道内的有益菌,抑制有害菌,调整或维持肠道菌群平衡,达到预防疾病,促进生长,提高饲料利用率的作用;酶制剂中含有多种可分解饲料中营养物质的酶,帮助动物消化吸收,促进动物生长,提高饲料利用率。 Is now probiotics and enzymes, probiotics by increasing the beneficial bacteria in the digestive tract, suppression of harmful bacteria, adjust or maintain the balance of intestinal flora, to prevent disease, promoting growth, improving feed utilization of action; Enzyme formulations containing more decomposable enzyme feed nutrients and help digestion and absorption of animal, promote animal growth, improve feed utilization. 但这些产品并不能完全达到饲料抗生素的应用效果。 However, these products do not fully meet the application effect of feed antibiotics. 中草药是我国传统的防病治病药物,现在人们将希望寄于中草药上用于动物的保健。 Chinese herbal medicine is a traditional disease prevention drugs, now people will want to send to the Chinese herbal medicine for the care of animals. 多数研究表明,中草药饲料添加剂具有促进畜禽生长和保健作用,且其毒副作用小、无残留、无耐药性等独特优势,但中药复方量大、吸收差,而提取物价格昂贵、功效单一,在应用上受到制约。 Most studies show that Chinese herbal additives can promote animal growth and health effects, and the toxic side effects, no residue, no drug resistance and other unique advantages, but the large amount of Chinese herbal compound, absorption is poor, and the extract is expensive, a single effect , restricted in the application.

[0012] 人用医药领域对中药有效成分与微量元素配位研究取得很大的进展,随着生物无机化学的迅速发展和对中药生理、药理活性物质的深入研究,大量的研究成果已将微量元素(无机成分)作为中药中重要的有效成分和对生命、健康及疗效的重要意义,予以了充分的认定。 [0012] people get the medical field with the active ingredients of traditional Chinese medicine and coordination of trace elements in a lot of progress, with the rapid development of bio-inorganic chemistry and in-depth study of the physiological medicine, pharmacologically active substance, a large number of studies have trace elements (inorganic component) as an important active ingredient in traditional Chinese medicine and of the importance of life, health and efficacy, to be fully identified. 对微量元素的研究也由单纯的测定,逐步深入。 The study of trace elements are also determined by the simple, gradually deepened. 根据中药有效成分与微量元素配位理论研究表明,微量元素与中草药中有机成分配合后有较强的药理活性,其活性有些比有机化合物还高。 The active ingredients of Chinese medicine theory ligand and trace elements show, trace elements and herbs in strong organic pharmacologically active ingredient complex which is higher than the activity of some organic compounds. 药理效用大多是结合形态,比单用无机盐容易透入组织内。 Most pharmacological effects are bound form, than single inorganic readily penetrate into the tissue. 对于植物提取物与微量元素配合物的在饲料行业的应用国内尚未见研究报道。 For plant extracts and trace elements complexes have not been reported in the application of domestic feed industry.

[0013] 随着全球食品安全意识的增强,在当前养殖条件下,如何在发挥动物最大生产性能的同时,又能够保证动物的健康,提高机体免疫能力及对疾病等外来因素的抵抗力等越来越成为畜禽生产的核心内容。 [0013] With the increased global awareness of food safety in the current farming conditions, how to play at the same time the largest animal performance, but also to ensure animal health, improve the immune capacity and resistance to diseases and other external factors such as the increasingly become the core content of livestock and poultry production. 所以本公司通过研究应用益生菌配合微量元素和中草药发酵而成的一种新型复合畜禽微生态制剂,用以替代抗生素,同时降低微量元素用量。 We therefore investigated application of probiotics with the new composite trace elements and poultry probiotics fermented Chinese herbal medicine as an alternative to antibiotics, while reducing the amount of trace elements. 此产品可通过改变肠道微生物区系,克服抗生素和化学药物的不良后果,有促生长、防病治病、保健等作用,能显著提高畜禽生产性能,提高饲料利用率等。 This product can be obtained by changing the intestinal microflora, to overcome adverse consequences of antibiotics and chemicals, have growth-promoting, disease prevention treatment, health care, can significantly improve the performance of livestock production, improve feed utilization. 这不仅能加强安全畜产品生产和环境保护,保障人民身体健康,同时又能促进畜牧业可持续发展,满足我国对外贸易的需要,也是国家政治经济稳定的需要。 This will not only strengthen the safety of animal production and environmental protection, protect people's health, while promoting the sustainable development of animal husbandry, meet the needs of China's foreign trade, the country also needs political and economic stability.

[0014] 在现有技术中,畜禽养殖产生了大量粪便,畜禽粪便中含有大量氮、磷和有机污染物等,经过发酵后会产生大量的氨氮、硫化氢、粪臭素、甲烷等有害气体,这些气体不但会破坏生态,而且还会直接影响人类健康。 [0014] In the prior art, produces a large number of livestock and poultry manure, manure contains large amounts of nitrogen, phosphorus and organic pollutants, after a large amount of harmful fermentation will produce ammonia, hydrogen sulfide, skatole, methane gas, which will not only destroy the ecology, but also a direct impact on human health. 另外畜禽养殖场(户)环境意识差,随意堆放畜禽粪便和排放污水,污物随雨水和污水进入水系,严重污染地下水,造成土质和水质不断恶化。 In addition environmental awareness poor livestock and poultry farms (households), the dumping of animal manure and sewage, waste and sewage into the river with the rain, polluted groundwater, causing soil and water quality is deteriorating. 养殖业带来的环境污染问题以引起高度重视,要认真吸取某些地区在畜牧业发展过程中存在的先污染后防治的教训。 The aquaculture industry has brought environmental pollution to attach great importance to conscientiously draw lessons after pollution prevention and control of certain areas exist in the animal husbandry development.

[0015] 此外,改革开放以来,我国畜牧业取得了长足的发展,目前我国的肉类产量跃居世界首位,已成为名副其实的畜牧大国。 [0015] In addition, since the reform and opening up, China has made considerable livestock development, China's meat production ranks first in the world, it has become a veritable animal husbandry big country. 但畜牧业在我国仍然是低产产业,为提高动物的生产性能,多用和滥用饲料添加剂(铜、锌、抗生素和促生产类添加剂)已成为饲料行业普遍存在的问题。 But in our country continues to be low-yielding livestock industry, to improve animal performance, the use and abuse of feed additives (copper, zinc, antibiotics and additives promote production class) has become a common problem in the feed industry. 国家也出台了一些相应的法律法规加以限制,但收效不尽如人意。 National also introduced a number of appropriate laws and regulations to limit, but the effect is not satisfactory. 这些添加剂一部分残留在畜产品当中,被食用直接影响人们的健康;一部分随动物排泄物直接进入环境,对土壤、空气、水源造成一定的污染,进而通过食物链进入人体,同样潜在威胁人类健康。 The remaining part of the additives in livestock products which were consumed directly affect people's health; part with animal waste directly into the environment, cause some contamination of soil, air, water, and then enter the body through the food chain, is also a potential threat to human health. 因此,如何解决畜牧业安全生产和对环境的影响已成为当前畜牧业可持续发展中亟待解决的问题。 So, how to solve the safety and impact of livestock production on the environment has become an issue in the current sustainable development of animal husbandry solved. 随着我国经济的高速发展,生态农业的推广,饲料行业对环保型产品的需求量越来越大。 With China's rapid economic development, promotion of ecological agriculture, feed industry demand for environmentally friendly products is growing.

发明内容 SUMMARY

[0016] 本发明的目的是提供一种保健发酵饲料配合益生菌发酵床的生态养殖方法,该方法中的中草药与微量元素经过发酵,形成一种新的化合物,可降低微量元素用量,且完全替代了抗生素,即节约资源,减少排放,保护环境,又提高产品品质。 [0016] The object of the present invention is to provide a method of ecological farming health fermented feed with the probiotic fermentation bed, herbs and trace elements in the process of fermentation, the formation of a new compound, can reduce the amount of trace elements, and fully alternative antibiotics that save resources, reduce emissions, protect the environment, and improve product quality. 且本发明的发酵床通过中草药与生态床结合使用弥补现有生态床的缺点,解决了霉菌、致病菌,寄生虫的发生等问题;且本发明有效的克服现有饲料使用铜源、高锌、高抗生素和促生长类添加剂带来的食品安全以及环境污染问题。 And the fermentation bed by the present invention to make up the bed and ecological herbal ecological disadvantages of the prior bed combination, solve the mold, bacteria, parasites and other problems occur; and the present invention is effective to overcome the existing copper feed source, high bring the zinc, the high antibiotics and growth promoters additives, food safety and environmental pollution problems.

[0017] 本发明的目的是通过以下技术方案来实现: [0017] The object of the present invention is achieved by the following technical solution:

[0018] 一种保健发酵饲料配合益生菌发酵床的生态养殖方法,所述生态养殖方法包括以下步骤: [0018] A health-fermented feed with ecological farming probiotics fermentation bed, said method comprising the steps of ecological farming:

[0019] ①有益菌种发酵:包括厌氧微生物的厌氧发酵和有氧微生物的有氧发酵;所述厌氧微生物包括植物乳酸菌和粪肠球菌dhf-5,所述有氧微生物包括白地霉菌、酿酒酵母菌、放线菌dha-8以及枯草芽孢杆菌dhk-7 ;所述厌氧发酵是将厌氧微生物接种到液体培养基中进行无氧恒温培养,并通过一级扩大培养和二级扩大培养得到厌氧发酵菌液;所述有氧发酵是将有氧微生物接种到液体培养基中进行有氧恒温培养,并通过一级扩大培养和二级扩大培养得到有氧发酵菌液益生菌发酵床的制备:将制备发酵床物料的各种原料混合均匀,得到有机垫料,再将厌氧发酵菌液和有氧发酵菌液接种至有机垫料中,进行堆积固态发酵,3-5天后当固体物料温度达到50°C时,将有机垫料均匀铺在所述发酵床上,所铺厚度不小于60cm,再对畜禽进行生态养殖;所述有机垫料包括锯末、稻壳 [0019] ① probiotic fermentation: aerobic fermentation include aerobic and anaerobic fermentation of microorganisms of anaerobic microorganisms; said anaerobic microorganisms include Lactobacillus plantarum and Enterococcus faecalis dhf-5, the aerobic microorganisms include fungi Geotrichum , Saccharomyces cerevisiae, actinomycetes dha-8 and Bacillus subtilis dhk-7; the anaerobic anaerobic fermentation is inoculated into a liquid medium for anaerobic incubation, cultured and expanded by two and a anaerobic fermentation broth culture to obtain expansion; the aerobic fermentation of aerobic microorganisms is inoculated into a liquid medium for aerobic incubation, culture and expansion by an expansion for two aerobic fermentation broth obtained probiotic preparation of fermentation bed: mixing various raw materials to prepare a fermentation bed material uniformly, to give an organic litter, then anaerobic fermentation and aerobic fermentation broth was inoculated into broth organic litter, for the accumulation of fermentation, 3-5 days later when the solid material reaches a temperature of 50 ° C, the organic litter spread evenly the fermentation bed, the lay thickness of not less than 60cm, and then the livestock ecological farming; the organic gasket comprises sawdust, rice husks 麸皮以及第一复方中草药,所述第一复方中草药包括蒲公英、鱼腥草、茵陈、秦皮、大青叶、淫羊藿、青蒿、穿心莲、龙葵、博落回、地锦草、贯众与土荆皮;③发酵饲料的成份:a.教槽保育饲料包括玉米50kg、豆柏35kg和小料15kg,其中小料包括维生素0.2kg、微量元素0.06kg、矿物质2.91kg、氨基酸1.2kg、其它添加剂9.13kg以及第二复方中草药1.5kg ;b.仔猪饲料包括玉米55kg、豆柏22kg、花生柏5kg、麸皮IOkg以及小料8kg,其中小料包括维生素0.2kg、微量元素0.05kg、矿物质2.91kg、氨基酸1kg、其它添加剂2.34kg以及第二复方中草药1.5kg ;c.中大猪饲料包括玉米55kg、豆柏12kg、棉柏4kg、麸皮15kg以及小料14kg ;其中小料包括维生素0.2kg、微量元素0.04kg、矿物质2.44kg、氨基酸0.8kg、其它添加剂9.02kg以及第二复方中草药 Bran and the first Chinese herbal compound, the first compound Chinese herbal medicine, including dandelion, Houttuynia, wormwood, ash bark, Folium, Epimedium, Artemisia annua, Andrographis, Solanum nigrum, Macleaya, Humifusa and rhizome pseudolaric; ③ fermented feed ingredients:. a creep feed include corn care 50kg, 35kg and small material beans Bo 15kg, wherein the feed comprises small vitamins 0.2kg, trace elements 0.06kg, minerals 2.91kg, amino acids 1.2kg, 9.13kg of other additives, and a second compound herbal 1.5kg; b piglet feed include corn 55kg, 22kg Bo beans, peanuts Bo 5kg, bran and small material IOkg 8kg, wherein the feed comprises small vitamins 0.2kg, trace elements 0.05. kg, minerals 2.91kg, amino 1kg, other additives, and a second compound herbal 2.34kg 1.5kg; c large swine feed include corn 55kg, 12kg beans Bo, Bo cotton 4kg, 15kg bran and small material 14kg;. wherein small binders include vitamins 0.2kg, trace elements 0.04kg, minerals 2.44kg, amino 0.8kg, 9.02kg of other additives, and a second compound DRUGS

1.5kg ;所述第二复方中草药包括杜仲、蒲公英、鱼腥草、茵陈、秦皮、大青叶、淫羊藿、青蒿、穿心莲、龙葵、地锦草、山楂、麦芽以及神曲;④发酵饲料的制备:a.菌种培育:按步骤①中所述厌氧发酵的方法来制得植物乳酸菌和粪肠球菌dhf-5的厌氧发酵的菌液;按步骤①中所述有氧发酵方法来制得酿酒酵母菌的有氧发酵菌液;b.将步骤③中所述的饲料中除小料外的其它饲料成份投入到卧式搅拌机中,边搅拌边蒸煮至80°C进行高温灭菌熟化,然后冷却至40°C以下后加入小料,均匀混合,并将加入了5%糖蜜的厌氧发酵菌液和酿酒酵母菌的有氧发酵菌液混合均匀,再接种至加入了小料的饲料中,堆积厌氧发酵3-5天,当pH< 4.5,停止发酵,得到发酵饲料。 1.5kg; the second compound Chinese herbal medicine, including gutta, dandelion, Houttuynia, wormwood, ash bark, Folium, Epimedium, Artemisia annua, Andrographis, Solanum nigrum, Verbena grass, hawthorn, malt and Divine Comedy; ④ preparation of fermented feed: a cultivate bacteria: the method of anaerobic fermentation of said bacteria be prepared by the steps ① fermentation of lactobacillus plantarum and Enterococcus faecalis obtained dhf-5; and in the aerobic step by step ① aerobic fermentation process the fermentation broth produced by Saccharomyces cerevisiae obtained; b. the step ③ said other feed ingredients in feed materials except a small horizontal mixer into, with stirring to 80 ° C for digestion. aging temperature sterilization, and then cooled to below 40 ° C were added small material, uniformly mixed and added to the anaerobic fermentation broth and 5% molasses aerobic fermentation broth of Saccharomyces cerevisiae mixed, then added to the inoculated small feed material in bulk 3-5 days anaerobic fermentation, when pH <4.5, the fermentation was stopped to give fermentation feed.

[0020] 进一步地,所述其它添加剂包括酸化剂等成份。 [0020] Further, the other additives include acidifying agents and other ingredients.

[0021]进一步地,步骤①中所述厌氧发酵的具体过程是将保存的植物乳酸菌、粪肠球菌dhf-5接种到液体培养基中,在无氧条件下37 °C恒温培养24h,当发酵液pH < 4.5时,将其移种至50L的发酵罐中进行一级扩大培养,在无氧条件下37°C恒温培养24h,当发酵罐内的发酵液PH < 4.5时,将其移种至1000L的发酵罐中进行二级扩大培养,无氧条件下37°C恒温培养30h,当发酵罐内的发酵液pH < 4.5时,结束发酵,得到所述的厌氧发酵菌液。 [0021] Further, the specific process in the step ① is to save the anaerobic fermentation of L. plantarum, Enterococcus faecalis dhf-5 was inoculated into the liquid medium, under anaerobic conditions at 37 ° C incubated 24h, when broth at pH <4.5, it is transplanted to the 50L fermenter for expanding a culture under anaerobic conditions at 37 ° C incubated 24h, when the fermentation broth in the fermenter PH <4.5, to move it 1000L fermenter seed to the expansion carried out in the two culture, anaerobic incubation conditions 37 ° C 30h, when the fermentation broth of the fermenter pH <4.5, the end of fermentation, anaerobic fermentation broth obtained according to.

[0022] 进一步地,步骤①中所述的有氧发酵的具体过程是将所述有氧微生物接种到液体培养基中,有氧条件下30°C恒温培养24h,当发酵液pH < 5.5时,将其移种至50L的发酵罐中进行一级扩大培养,在有氧条件下30°C恒温培养24h,当罐内发酵液pH < 5.5时,将其移种至1000L的发酵罐中进行二级扩大培养,在有氧条件下30°C恒温培养30h,当发酵罐内发酵液pH < 5.5时,结束发酵,得到所述的有氧发酵菌液。 [0022] Further, in the step ① aerobic fermentation process in particular aerobic microorganisms was inoculated into the liquid medium, under aerobic conditions of 30 ° C incubation 24h, when the fermentation broth pH <5.5 when which was transplanted to 50L fermenter for an expansion in culture under aerobic conditions at 30 ° C incubation 24h, when the tank fermentation broth pH <5.5 when it is to be transplanted in 1000L fermenter two expansion culture under aerobic conditions at 30 ° C 30h incubation, when the fermenter broth pH <5.5, the end of fermentation, the fermentation broth obtained according to aerobic.

[0023] 进一步地,所述厌氧发酵和有氧发酵中所用的液体培养基的成份包括:葡萄糖、硫酸氨、硫酸锰、硫酸镁、磷酸二氢钾、玉米、豆柏以及去离子水。 [0023] Further, the liquid composition of the fermentation medium used in the aerobic and anaerobic fermentation include: glucose, ammonium sulfate, manganese sulfate, magnesium sulfate, potassium dihydrogen phosphate, corn, beans, Bo and deionized water.

[0024] 进一步地,步骤②中所述有机垫料的成份及其重量份数包括40%的锯末、40%的稻壳、5%的麸皮以及15%的第一复方中草药,所述第一复方中草药中各种成份的重量份数为:蒲公英1-2份、鱼腥草1-2份、茵陈1-2份、秦皮1-2份、大青叶1-2份、淫羊藿1_2份、青蒿1-2份、穿心莲1-2份、龙葵1-2份、博落回2-4份、地锦草1-2份、贯众1_3份与土荆皮1-4份。 [0024] Further, in the step ② of the gasket organic ingredients and comprising 40% by weight of sawdust, rice hull 40%, 5% and 15% of the bran first compound Chinese herbal medicine, the second a compound Chinese herbal parts by weight of various components is: 1-2 parts of dandelion, Houttuynia parts 1-2, 1-2 parts of wormwood, ash bark parts 1-2, 1-2 parts Folium, Epimedium 1_2 parts of beans, 1-2 parts of Artemisia annua, Andrographis 1-2 parts, 1-2 parts of Solanum nigrum, Macleaya 2-4 parts humifusa 1-2 parts, parts and rhizome 1_3 pseudolaric 1- 4 parts. [0025] 进一步地,步骤②中所述的厌氧发酵菌液和有氧发酵菌液是以1:1的体积比混合均匀,再按10% (质量百分数)的接种量将其接种至有机垫料中。 [0025] Further, in the step ② of the anaerobic fermentation and aerobic bacteria fermentation broth is 1: 1 volume ratio mixed uniformly, then 10% (by mass percent) of the inoculated inoculated to organic the litter.

[0026] 进一步地,接种后的所述有机垫料的含水量不超过60%。 [0026] Further, the water content of the organic inoculated dunnage does not exceed 60%.

[0027] 进一步地,步骤②中所述的发酵床的大小为不小于20m2,所述畜禽为猪,平均每头猪所占面积为1.0~1.5m2。 [0027] Further, ② the step size in said fermentation bed is not less than 20m2, the livestock is a pig, the mean area occupied by each pig 1.0 ~ 1.5m2.

[0028] 进一步地,步骤③中所述的教槽保育饲料中的微量元素的成份及其含量分别为:碱铜6.7克、硫酸亚铁33.4克、硫酸锰9.5克、氧化锌11.7克、碘化钾0.046克以及亚硒酸钠0.068克;所述的仔猪饲料中的微量元素的成份及其含量分别为:碱铜5.17克、硫酸亚铁26.7克、硫酸锰9.5克、氧化锌9.3克、碘化钾0.046克以及亚硒酸钠0.068克;所述的中大猪饲料中的微量元素的成份及其含量分别为:碱铜3.88克、硫酸亚铁20克、硫酸锰 [0028] Further, in the step ③ said creep feed care ingredients and trace elements content are: alkaline copper 6.7 g, 33.4 g of ferrous sulfate, manganese sulfate 9.5 g, 11.7 g of zinc oxide, potassium iodide 0.046 g and 0.068 g of sodium selenite; the piglet feed composition and content of trace elements are: alkaline copper 5.17 g, 26.7 g of ferrous sulfate, manganese sulfate 9.5 g, 9.3 g of zinc oxide, potassium iodide 0.046 g and 0.068 g sodium selenite; large pig feed the ingredients in trace elements and content are as follows: 3.88 g of copper base, 20 g of ferrous sulfate, manganese sulfate

9.5克、氧化锌7克、碘化钾0.046克以及亚硒酸钠0.068克。 9.5 g, 7 g of zinc oxide, 0.046 g of potassium iodide and 0.068 g of sodium selenite.

[0029] 进一步地,步骤③中所述的第二复方中草药中各种成份及其重量份数为杜仲10-75份、蒲公英1-20份、鱼腥草1-30份、茵陈1_30份、秦皮1_30份、大青叶1_30份、淫羊藿1-30份、青蒿10-60份、穿心莲1-30份、龙葵10-50份、地锦草1-30份、山楂10-50份、麦芽10-50份、神曲10-50份。 [0029] Further, in the step ③ said second compound Chinese herbal medicine in various components and parts 10-75 parts by weight of eucommia, 1-20 parts of dandelion, Houttuynia 1-30 parts, parts capillaris 1_30 Qin skin 1_30 part, Folium 1_30 parts, epimedium 1-30 parts, Artemisia annua 10 to 60 parts, 30 parts Andrographis, Solanum nigrum 10-50 parts, humifusa 1-30 parts, hawthorn 10- parts 50, 10 to 50 parts malt, Shinkyoku 10 to 50 parts.

[0030] 进一步地,步骤④中所述的发酵饲料制备的具体过程为:a.将除小料外的其它饲料成份投入到卧式搅拌机中,边搅拌边蒸煮至温度80°C进行高温灭菌熟化,保持20分钟左右;再用提升机输送到旋转式搅拌机,冷却,待温度降低至40°C以下后,在其中均匀加入小料,与灭菌后的其它饲料成份均匀混合4~7分钟,优选5分钟;b.向以体积比1:1混合后的步骤③所述的厌氧发酵菌液和酿酒酵母菌的有氧发酵菌液中加入5% (指体积百分数)的糖蜜,混合均匀得接种物;c.将步骤b中所述的接种物按10% (指质量百分数)的接种量接种至步骤a中混合了小料的饲料中,堆积厌氧发酵3-5天,发酵至pH < 4.5时,停止发酵,得到发酵饲料,即发酵至有酒香味时停止发酵,便可用于饲喂畜禽。 [0030] Further, the specific process of fermented feeds prepared in the step ④ of:. A the other ingredients except the feed material into a small horizontal mixer with stirring to a temperature of 80 ° C cooking temperature for Off strain aging, held about 20 minutes; and then to lift conveyor rotary agitator, cooling until the temperature drops to below 40 ° C after, addition of even small material therein, mixed uniformly with 4-7 sterilized other feed ingredients minutes, preferably 5 minutes; to B at a volume ratio 1: ③ a step after mixing the anaerobic bacteria and aerobic fermentation of the fermentation broth of Saccharomyces cerevisiae added 5% (volume percent means) molasses. uniformly mixed to obtain inoculum;. c in said step b inoculum 10% (means mass percent) of the inoculum was inoculated into a mixing step small feed material, the accumulation of anaerobic fermentation for 3-5 days, fermentation to pH <4.5, the fermentation was stopped to give fermentation feed, i.e. to stop the fermentation when fermented wine flavor, can be used for feeding livestock and poultry.

[0031] 进一步地,接种后的饲料的含水量为40~50%,优选含水量为45%。 [0031] Further, the water content of the inoculated feed is 40 to 50%, preferably 45% water content.

[0032] 本发明提供了一种保健发酵饲料配合益生菌发酵床的生态养殖方法,本发明的关键技术点是通过将中草药等有机垫料进行发酵,并将其添加至发酵床中对畜禽进行养殖,而饲料方面,将包括中草药和微量元素以及其它成份一起进行发酵,使得使用该技术所养殖的畜禽与现有技术相比,主要具有以下几点突出的有益效果。 [0032] The present invention provides a method of ecological farming health probiotic fermented feed fermentation bed with the key technical point of the present invention is carried out by fermentation of organic herbs and other litter, and added to the fermentation bed for livestock for breeding, and feed aspects, including herbs and trace elements and other components with fermentation, such prior art livestock and breeding using this technology as compared to the projecting points has the following advantageous effects. [0033] ①中草药与微量元素结合具有抗病和促生长的功效,本发明所述方法完全可以替代抗生素,同时降低微量元素用量。 [0033] ① herbs having antiviral efficacy in combination with trace elements and growth performance, the method of the present invention can replace antibiotics, while reducing the amount of trace elements.

[0034] ②降低微量元素用量,节约资源,减少排放,保护环境。 [0034] ② reduce the amount of trace elements, save resources and reduce emissions for environmental protection.

[0035] ③无抗生素,无残留,保证食品安全和人类健康。 [0035] ③ no antibiotics, no residue, ensuring food safety and human health.

[0036] ④净化水质,阻断由水源给猪只带来的健康隐患。 [0036] ④ water purification, water blocked by the pigs to bring health risks.

具体实施方式 detailed description

[0037] 本发明实施例所述的一种保健发酵饲料配合益生菌发酵床的生态养殖方法,下面以具体实验案例为例来说明具体实施方式,应当理解,此处所描述的具体实施例仅仅用以解释本发明,并不用于限定本发明。 [0037] A health fermented feed according to embodiments of the present invention with the embodiment of ecological farming probiotics fermentation bed, the following concrete experimental example to illustrate the case of specific embodiments, it should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. [0038] 实施例1:有益菌种的种类及其发酵方法 Species and beneficial bacteria fermentation methods: one case of [0038] Embodiment

[0039] 本发酵床使用菌种包括植物乳酸菌、粪肠球菌dhf-5、白地霉菌、酿酒酵母菌、放线菌dha-8以及枯草芽孢杆菌dhk-7,将植物乳酸菌和粪肠球菌dhf-5进行厌氧发酵,其它菌种进行有氧发酵。 [0039] The present fermentation bed comprising Lactobacillus plantarum strains, Enterococcus faecalis dhf-5, fungus Geotrichum, Saccharomyces cerevisiae, actinomycetes dha-8 and Bacillus subtilis dhk-7, the Lactobacillus plantarum and Enterococcus faecalis dhf- 5 anaerobic fermentation, aerobic fermentation of strains other.

[0040] 厌氧发酵:将保存完好的植物乳酸菌和粪肠球菌dhf-5接种到液体培养基中(1000mL锥形瓶装量一般为500mL)在厌氧环境下,即在无氧条件下37°C恒温培养24h,当发酵液pH <4.5时,将其移种至50L的发酵罐中进行一级扩大培养,然后在厌氧(即无氧)条件下37°C恒温培养24h,当发酵罐内的发酵液pH < 4.5时,再将其移种至1000L的发酵罐中进行二级扩大培养,再在无氧条件下,37°C恒温培养30h,当发酵罐内的发酵液pH < 4.5时,液体发酵结束,得到厌氧发酵的菌液。 [0040] anaerobic fermentation: The preserved L. plantarum and Enterococcus faecalis dhf-5 was inoculated into a liquid medium (typically an amount of 1000mL Erlenmeyer flask 500 mL) under anaerobic conditions, i.e., under anaerobic conditions at 37 ° C incubation 24h, when the fermentation broth pH <4.5, it will move 50L seed fermenter to be in an expansion culture, and anaerobic (i.e., anaerobic) at 37 ° C incubation conditions for 24h, when a fermentor in the fermentation broth pH <4.5, and then move it for two kinds of culture to expand the 1000L fermenter, and then under anaerobic conditions, 37 ° C incubation 30h, when a fermentor broth pH <4.5 when the liquid end of fermentation, anaerobic fermentation broth obtained.

[0041] 有氧发酵:将保存的白地霉菌、酿酒酵母菌、放线菌dha-8以及枯草芽孢杆菌dhk-7接种到液体培养基中(1000mL锥形瓶装量为500mL),有氧环境下,30°C恒温培养24h,当发酵液pH < 5.5时,将其移种至50L的发酵罐中进行一级扩大培养,然后在有氧条件下,30°C恒温培养24h,当发酵罐内的发酵液pH < 5.5时,将其移种至1000L的发酵罐中进行二级扩大培养,有氧条件下,30°C恒温培养30h,当发酵罐内发酵液pH < 5.5时,液体发酵结束,得到有氧发酵的菌液。 [0041] aerobic fermentation: the saved white, molds, Saccharomyces cerevisiae, actinomycetes dha-8 and Bacillus subtilis dhk-7 was inoculated into the liquid medium (1000mL Erlenmeyer flask in an amount of 500 mL), under aerobic conditions , 30 ° C incubation 24h, for expanding a culture broth when pH <5.5 when it is transplanted to a 50L fermenter, and then under aerobic conditions, 30 ° C incubation 24h, when the fermentation tank broth pH <5.5, for which two transplanting cultured to expand the 1000L fermenter, under aerobic conditions, 30 ° C incubation 30h, when the fermenter broth pH <5.5, the end of the liquid fermentation , get aerobic fermentation broth.

[0042] 优选地,上述厌氧发酵和有氧发酵中所用的液体培养基的成份包括:葡萄糖、硫酸氨、硫酸锰、硫酸镁、磷酸二氢钾、玉米、豆柏以及去离子水等成份。 [0042] Preferably, the composition of the fermentation broth used in the aerobic and anaerobic fermentation include: glucose, ammonium sulfate, manganese sulfate, magnesium sulfate, potassium dihydrogen phosphate, corn, beans, Bo and deionized water and other ingredients .

[0043] 发酵床的制备:先将重量百分比分别为40 %的锯末、40 %的稻壳、5 %的麸皮、15%的第一复方中草药混合均匀,即为有机垫料,再将厌氧发酵的菌液和有氧发酵的菌液以体积比1:1混合,按10%的接种量将混合后的菌液接种至所述的有机垫料中,含水量控制在不超过60%,然后以堆积的方式进行固态发酵,3-5天后当固体物料温度达到50°C时,将发酵后的有机垫料以厚度不小于60cm铺在不小于20m2的发酵床上,对猪进行生态养殖,密度为平均每头猪占1.0m2~1.5m2。 Preparation [0043] The fermentation bed: the first weight percentage respectively of 40% sawdust, 40% rice hull, wheat bran 5%, 15% of the first compound mixed herbs, namely organic litter, then tired bacteria and aerobic bacteria fermented fermented oxygen volume ratio of 1: 1 mixture of 10% of the inoculum broth to the mixed inoculation of the organic litter, water content of not more than 60% and then stacked manner fermentation, 3-5 days later when the solid material reaches a temperature of 50 ° C, the fermented organic litter thickness not less than 60cm in order to spread the fermentation bed is not less than 20m2, pig farming ecological a density of pigs per account 1.0m2 ~ 1.5m2. 该第一复方中草药为80目的粉末。 The first compound 80 for the purpose herbal powder.

[0044] 本发明采用益生菌发酵床的生态养殖方法彻底解决养猪对环境的污染问题。 [0044] The present invention utilizes probiotics fermentation bed ecological farming methods to solve environmental pollution problems pig. 是由于在上述发酵床上所铺的有机垫料里含有具有活性的有益微生物(即上述的几种菌种),其能够迅速有效地降解、消化猪的粪尿排泄物,不需要每天清扫猪栏,冲洗猪舍,从而没任何废弃物排出猪场,也没有任何冲洗圈舍的污水,可节省用水70%以上。 Due to containing the beneficial microorganism (i.e., several of these strains) having the above activity of the fermentation bed in the shop where the organic litter, which can be quickly and efficiently degraded, digested excreta of pigs, pigsty not require daily cleaning , flushing pig to pig without any discharge of waste, and no flush pens sewage, can save 70% or more water. 真正达到养猪零排放的目的。 The real purpose of swine zero emissions. 同时改善猪舍环境,因带有发酵床的猪舍设计为大窗,夏季全开放冬季卷帘封闭保温, 猪舍房顶设置电动排气天窗,通风透气好、温湿度均适合于猪的生长。 While improving pig house, because the pig is designed to fermentation bed with large windows, all open in summer and winter insulation shutter closed, power is provided an exhaust barn roof skylight, good ventilation, temperature and humidity are suitable for the growth of pigs . 猪粪尿经有益微生物迅速分解,猪舍里没有臭气、不滋生苍蝇,经环境空气检测,微生态发酵床猪舍内二氧化碳、硫化氢、氨气等有害气体含量均低于传统暖棚猪舍,大大低于国家农业部《畜禽场环境质盘标准》。 Pig manure rapid decomposition by beneficial microorganisms, pigsty no odor, non-breeding flies, by detecting the ambient air, the probiotics fermentation bed pig harmful gas content of carbon dioxide, hydrogen sulfide, ammonia and the like are lower than traditional greenhouses pigs homes, well below the state Department of agriculture 'Animal farm environment quality disc standard. " 此外,在微生态的发酵床中使用第一复方中草药粉,对发酵床进行进一步改进,以防止滋生霉菌、致病菌及寄生虫等,大大降低了猪的发病率,有效解决了当猪场和猪群发生呼吸道疾病时,如果不用药则猪生长速度缓慢,成活率和生长速度低、饲料报酬降低,利益得不偿失的不足。 Further, the use of probiotics in fermented Chinese herbal powder bed of a first compound, further improvement of the fermentation bed, in order to prevent the breeding of mildew, bacteria and parasites, which greatly reduced the incidence of pig farms effective solution when and when respiratory diseases in pigs occur if the medication is not swine slow growth, low survival rate and growth rate, lower feed conversion, lack of interest more harm than good.

[0045] 此外,要注意,在该设有发酵床养猪猪舍内不能使用化学消毒药品和抗生素类药物,如果使用,将杀灭和抑制微生物,或抑制其繁殖,使得微生物的活性降低;这也有利于防止人们使用抗生素等物质来饲养猪。 [0045] Further, it is noted that the features can not be used in the fermentation pig barn chemical disinfectant and antibiotics, if used, and to inhibit microbial kill, or inhibit reproduction, such decreased activity of the microorganisms; it is also helpful to prevent people from using antibiotics and other substances used to raise pigs.

[0046] 实施例2:发酵饲料及其制备 [0046] Example 2: Preparation of fermented feed and

[0047] ①饲料成份:发酵饲料的成份:a.教槽保育饲料包括玉米50kg、豆柏35kg和小料15kg,其中小料包括维生素0.2kg、微量元素0.06kg、矿物质2.91kg、氨基酸1.2kg、其它添加剂9.13kg以及第二复方中草药1.5kg ;b.仔猪饲料包括玉米55kg、豆柏22kg、花生柏5kg、麸皮IOkg以及小料8kg,其中小料包括维生素0.2kg、微量元素0.05kg、矿物质 [0047] ① feed ingredients: fermented feed ingredients: a creep feed include corn care 50kg, 35kg and small material beans Bo 15kg, wherein the feed comprises small vitamins 0.2kg, trace elements 0.06kg, minerals 2.91kg, amino acids 1.2. kg, 9.13kg, and a second compound other additives herbal 1.5kg; b piglet feed include corn 55kg, 22kg Bo beans, peanuts Bo 5kg, bran and small material IOkg 8kg, wherein the feed comprises small vitamins 0.2kg, trace elements 0.05kg. , minerals

2.91kg、氨基酸1kg、其它添加剂2.34kg以及第二复方中草药1.5kg ;c.中大猪饲料包括玉米55kg、豆柏12kg、棉柏4kg、麸皮15kg以及小料14kg ;其中小料包括维生素0.2kg、微量元素0.04kg、矿物质2.44kg、氨基酸0.8kg、其它添加剂9.02kg以及第二复方中草药1.5kg。 2.91kg, amino acids 1kg, other additives, and a second compound herbal 2.34kg 1.5kg; c Large swine feed include corn 55kg, 12kg beans Bo, Bo cotton 4kg, 15kg bran and small material 14kg;. Wherein small materials include vitamins 0.2 kg, trace elements 0.04kg, minerals 2.44kg, amino 0.8kg, 9.02kg of other additives, and a second compound herbal 1.5kg.

[0048] 其中,所述的教槽保育饲料中的微量元素的成份及其含量分别为:碱铜6.7克、硫酸亚铁33.4克、硫酸锰9.5克、氧化锌11.7克、碘化钾0.046克以及亚硒酸钠0.068克;所述的仔猪饲料中的微量元素的成份及其含量分别为:碱铜5.17克、硫酸亚铁26.7克、硫酸锰9.5克、氧化锌9.3克、碘化钾0.046克以及亚硒酸钠0.068克;所述的中大猪饲料中的微量元素的成份及其含量分别为:碱铜3.88克、硫酸亚铁20克、硫酸锰9.5克、氧化锌7克、碘化钾0.046克,亚硒酸钠0.068克。 [0048] wherein said creep feed care ingredients and trace elements content are: alkaline copper 6.7 g, 33.4 g of ferrous sulfate, manganese sulfate 9.5 g, 11.7 g of zinc oxide, 0.046 g of potassium iodide and ethylene sodium selenate 0.068 g; of the piglet feed composition and content of trace elements are: alkaline copper 5.17 g, 26.7 g of ferrous sulfate, manganese sulfate 9.5 g, 9.3 g of zinc oxide, 0.046 g of potassium iodide and selenious 0.068 g sodium; large pig feed the ingredients in trace elements and content are as follows: 3.88 g of copper base, 20 g of ferrous sulfate, manganese sulfate 9.5 g, 7 g of zinc oxide, 0.046 g of potassium iodide, ethylene 0.068 g of sodium selenate.

[0049] 其中,教槽保育饲料中的所述第二复方中草药中各种成份的量为杜仲0.1-0.75kg、蒲公英0.01-0.2kg、鱼腥草0.01-0.3kg、茵陈0.01-0.3kg、秦皮0.01-0.3kg、大青叶0.01-0.3kg、淫羊藿0.01-0.3kg、青蒿0.1-0.6kg、穿心莲0.01-0.3kg、龙葵 [0049] wherein the amount of creep feed conservation second compound Chinese herbal medicine Eucommia various components is 0.1 to 0.75 kg, dandelion 0.01-0.2kg, Houttuynia 0.01-0.3kg, Artemisia 0.01-0.3kg Qin skin 0.01-0.3kg, Folium 0.01-0.3kg, Epimedium 0.01-0.3kg, Artemisia annua 0.1-0.6kg, Andrographis 0.01-0.3kg, Solanum nigrum

0.1-0.5kg、地锦草0.01-0.3kg、山楂0.1-0.5kg、麦芽0.1-0.5kg、神曲0.1-0.5kg ;所述仔猪饲料中所述的第二复方中草药中各种成份的量为杜仲0.1-0.45kg、蒲公英0.01-0.2kg、鱼腥草0.01-0.3kg、茵陈0.01-0.3kg、秦皮0.01-0.3kg、大青叶0.01-0.3kg、淫羊藿 0.1-0.5kg, Humifusa 0.01-0.3kg, hawthorn 0.1-0.5kg, malt 0.1-0.5kg, Shinkyoku 0.1-0.5kg; piglet feed amount of the compound in the second component of various herbal gutta 0.1-0.45kg, dandelion 0.01-0.2kg, Houttuynia 0.01-0.3kg, wormwood 0.01-0.3kg, Qinpi 0.01-0.3kg, Folium 0.01-0.3kg, Epimedium

0.01-0.3kg、青蒿0.1-0.4kg、穿心莲0.01-0.3kg、龙葵0.1-0.5kg、地锦草0.01-0.3kg、山楂0.1-0.4kg、麦芽0.1-0.4kg、神曲0.1-0.4kg ;所述中大猪饲料中所述的第二复方中草药中各种成份的量为杜仲0.1-0.6kg、蒲公英0.01-0.2kg、鱼腥草0.01-0.3kg、茵陈 0.01-0.3kg, Artemisia annua 0.1-0.4kg, Andrographis 0.01-0.3kg, Solanum nigrum 0.1-0.5kg, humifusa 0.01-0.3kg, hawthorn 0.1-0.4kg, malt 0.1-0.4kg, The Divine Comedy 0.1-0.4kg ; large amount of pig feed said second compound Chinese herbal medicine for the various components of Eucommia 0.1-0.6kg, dandelion 0.01-0.2kg, Houttuynia 0.01-0.3kg, capillaris

0.01-0.3kg、秦皮0.01-0.3kg、大青叶0.01-0.3kg、淫羊藿0.01-0.3kg、青蒿0.1-0.6kg、穿心莲0.01-0.3kg、龙葵0.1-0.5kg、地锦草0.01-0.3kg、山楂0.1-0.4kg、麦芽0.1-0.4kg、神曲0.1-0.4kgο 0.01-0.3kg, Qin skin 0.01-0.3kg, Folium 0.01-0.3kg, Epimedium 0.01-0.3kg, Artemisia annua 0.1-0.6kg, Andrographis 0.01-0.3kg, Solanum nigrum 0.1-0.5kg, humifusa 0.01-0.3kg, hawthorn 0.1-0.4kg, malt 0.1-0.4kg, The Divine Comedy 0.1-0.4kgο

[0050] 这三个阶段的饲料均不含抗生素;该第二复方中草药为80目的中草药粉末。 [0050] The three phases are antibiotic-free feed; the second object compound Chinese herbal 80 herbal powder. 小料中与第二复方中草药结合的微量元素用量与正常用量相比,铜可降低80%以上(现有技术教槽保育阶段到中猪阶段饲料中的铜元素用量为150-200ppm,本发明中铜元素用量为22.5-37.5ppm),铁可降低45%以上(现在技术教槽保育阶段至中大猪阶段中铁元素用量为90-200ppm,本发明中铁元素用量60-100ppm),小猪阶段(即现有技术中教槽保育饲料中的锌元素用量为2115ppm,本发明中锌元素用量为87.5ppm)锌用量可降低90%以上,中大猪阶段锌用量可降低50%以上(现在技术中大猪阶段锌元素用量80-150ppm,本发明中锌元素用量为52.5-70ppm)等。 Small amounts of trace elements and a second batch of compound herbal binding compared to normal amounts, can be reduced by more than 80% copper (prior art creep conservation stage to the amount of copper in pig feed stage 150-200ppm, the present invention copper in an amount of 22.5-37.5ppm), iron can be reduced by 45% (creep nursery stage technology now to big pig iron phase in an amount of 90-200ppm, the amount of iron present invention 60-100ppm), pig stage (i.e., the amount of zinc in the prior art creep feed conservation is 2115ppm, the present invention in an amount of 87.5 ppm zinc) of zinc reduces the amount of more than 90%, large amounts pig zinc stage can be reduced by 50% (now technology large amounts of zinc pig stage 80-150ppm, the amount of zinc present invention 52.5-70ppm) and the like.

[0051] ②菌种培育:a.按实施例1中厌氧发酵的方法来培育植物乳酸菌和粪肠球菌dhf-5,得到和实施例1中相同的厌氧发酵的菌液。 [0051] ② cultivate bacteria: a method described in Example 1 to anaerobic fermentation and cultivated plants Lactobacillus Enterococcus faecalis dhf-5, obtained in Example 1 and the same anaerobic fermentation broth embodiment. b.按实施例1中有氧发酵的方法来培育酿酒酵母菌,得到酿酒酵母菌的有氧发酵菌液。 b. Press embodiment the method of Example 1 to foster aerobic fermentation of Saccharomyces cerevisiae, to give aerobic fermentation broth of Saccharomyces cerevisiae.

[0052] ③饲料发酵:将猪各阶段所喂饲料进行粉碎后,按配方称取,先将除小料外的其它饲料投入到500型卧式搅拌机中、边搅拌边蒸煮至温度80°C下进行高温灭菌熟化,保持20分钟;再用提升机输送到旋转式搅拌机,冷却;待温度降低至40°C以下后,向其它饲料中均匀加入小料,均匀混合5分钟左右,再将厌氧发酵的菌液和酿酒酵母菌的有氧发酵菌液以体积比1:1混合,并向该混合后的菌液中加入5%的糖蜜,再按10%的接种量将其接种至饲料中并混合均匀,含水量控制在45%,堆积厌氧发酵3-5天,当pH < 4.5时,有酒香味时就可以用于饲喂猪等畜禽。 [0052] ③ Feed Fermentation: The pigs are fed feed each stage after pulverization, weighed according to the recipe, except for the small first other feed materials into 500 horizontal mixer with stirring to a temperature of 80 ° C cooking sterilized at a high temperature curing, 20 min; then conveyed to the elevator rotary agitator, cooling; after the temperature was reduced to below 40 ° C, even a small added to other feed material, uniformly mixed for about 5 minutes, and then anaerobic fermentation of aerobic bacteria and Saccharomyces cerevisiae fermentation broth at a volume ratio of 1: 1 were mixed, and 5% molasses broth after the mixing, then 10% of the inoculum was inoculated to and uniformly mixed feed, water content of 45%, a bulk 3-5 days anaerobic fermentation, when pH <4.5, when the wine flavor can be used for feeding pigs and other livestock.

[0053] 本生态养殖全程饲喂上述发酵饲料,发酵饲料是经本发明申请人筛选的微生物通过其自身的代谢活动,将植物性、动物性和矿物性物质的饲料中的抗营养因子分解或转化,生产更易被动物采食、消化、吸收的养分。 [0053] The present ecological farming fed throughout the above fermented feed, feed fermenting microorganisms of the present invention by the applicant screened by its own metabolic activity, will feed vegetable, animal and mineral substances or decomposition of the anti-nutritional factors transformation, production of animal feed more easily, digestion, nutrient absorption.

[0054] 复方中药的功效不仅与其所含微量元素的数量有关,还与某些微量元素的含量比有关,关于这些前人已有不少研究。 [0054] The effect of compound Chinese medicine and its not only the number of trace elements contained, but also with the content of certain trace elements than about, there are many of these previous studies. 而利用微生物转化中草药来提高药效,是一种很好的思路,中草药发酵的目的一方面增强中药的疗效、改变药物原有性能、扩大用药品种,另一方面还可以充分利用微生物所产生的酶、酸、多糖等保健成分帮助药物、食物消化吸收,进一步促进动物恢复健康,提闻生广力。 The use of microbial transformation to improve the efficacy of Chinese herbal medicine, is a good idea, on the one hand the purpose of enhancing the efficacy of Chinese herbal medicine fermentation, changing the original properties of the drug, expand drug varieties, on the other hand can also take advantage produced by the microorganisms health components of enzymes, acids, polysaccharides and other drugs to help food digestion and absorption, to further promote the animal back to health, to mention Wensheng wide force.

[0055] 实施例3 [0055] Example 3

[0056] 试验目的:本试验通过采用低铜、铁、锌等微量元素加中草药,不使用抗生素与高铜、闻锋、闻抗生素做对比实验,对猪生广性能相关指标进行测定,检验其对猪生广性能的影响。 [0056] Test Objective: The test trace element through the use of herbal low copper, iron, zinc, and without the use of antibiotics, high copper, Wen Feng, smell antibiotics do comparative experiments, pigs born wide performance metrics were determined, which test wide impact on pig performance students. 试验地点及时间:选择试验猪场:自由猪场,试验时间从2011年5月9日至2011年9月26日,实验期141天,分两个阶段。 Test place and time: Select the test farm: free farms, test time from May 9, 2011 to September 26, 2011, the experimental period 141 days, in two stages.

[0057] 试验动物及饲料:选择80头同期出生的28日龄断奶、体重(实测)为8.315kg左右的仔猪实验,按饲养场常规管理,常规免疫,将所有猪只按窝别、性别随机分组。 [0057] Test Animals and feed: Select 80 28-day-old born in the same period of weaning weight (measured) of about 8.315kg piglet experiments, according to conventional farm management, routine immunization, according to all the pigs do not nest, random sex grouping. 饲料按生产实际或推荐配方配置,同一阶段各组基础饲料相同。 Feed formula by actual production or recommended configuration, the same basic diet each group the same stage.

[0058] 试验设计与饲养管理:试验分为两个阶段,乳仔猪阶段和中大猪阶段。 [0058] Experimental Design and feeding management: test phase is divided into two stages big pig, piglets and stage. 试验为连续性饲养。 It is a continuous feeding test. 以下表1~4中的ppm均为元素含量;以下四个表中,处理I组试验采用水泥地饲养,试验前用百毒杀对栏舍进行彻底消毒,试验期保持猪自由采食(大料部分原料经过发酵),自由饮水(经过净化),舍内地面保持干燥。 The following Table 1 ~ 4 ppm are element content; in the following four tables, I treated group using concrete feeding test, a test 100 poisoned before thorough disinfection of the pig bed, holding the test of porcine ad libitum (large fermentation feedstock feed section), consisting of water (after purification), dry ground discounted. 根据环境温度变化,保持保温工作。 The changes in the ambient temperature, maintaining insulation work. 按常规免疫程序免疫,正常搞好环境卫生,猪只发病时及时常规治疗。 By routine immunization program immunization, normal environmental sanitation, while the incidence of pigs timely conventional therapy. 处理2组试验采用本发明所述的发酵床饲养,试验期猪自由采食,自由饮水。 Test 2 treatment groups using fermentation bed feeder of the present invention, of the ad libitum test pigs, free access to water. 按常规免疫程序免疫。 Immunity by routine immunization program.

[0059] 乳仔阶段:随机分为2个处理,每个处理4个重复,每个重复10头猪,分养在8栏内,进行饲养试验,乳仔阶段包括断奶后2周与仔猪阶段,见表1和表2。 [0059] Milk Aberdeen stages: randomly divided into two treatments with four replicates of 10 pigs, 8 points raised in column breeding experiment, Aberdeen phase comprises milk two weeks after weaning piglets stage table 1 and table 2. 中大猪阶段(即中猪阶段和大猪阶段):延续乳仔猪阶段试验分组,试验分为2个处理,每个处理4个重复,每个重复10头猪,分养在8栏内,进行饲养试验,见表3和表4。 Large pig stage (i.e., stage pig and big pig phase): continuation of a packet milk piglets stage trial, the test is divided into two treatments with four replicates of 10 pigs, kept in points 8 column, feeding experiment performed, in tables 3 and 4.

[0060]表1 [0060] TABLE 1

[0061] [0061]

Figure CN103891674AD00121

[0062]表 2 [0062] TABLE 2

[0063] [0063]

Figure CN103891674AD00122

[0064]表 3 [0064] TABLE 3

[0065] [0065]

Figure CN103891674AD00123

[0066] 表4 [0066] TABLE 4

[0067] [0067]

Figure CN103891674AD00124

[0068] 下表5~7为上述试验的试验结果。 [0068] Tables 5 to 7 under test is the test result.

[0069] 表5:乳仔猪生产性能指标(5月8日-6月11日)35天 [0069] Table 5: piglets production performance indicators (5 8 - June 11) 35 days

[0070] [0070]

Figure CN103891674AD00125
Figure CN103891674AD00131

[0071] 表6:中猪生产性能指标(6月12日-9月23日)102天 [0071] Table 6: Performance of pigs (6 12 - September 23) 102 days

[0072] [0072]

Figure CN103891674AD00132

[0073] 表7皮毛、粪便颜色及不消化颗粒观测结果 [0073] Table 7 coat, and stool color observations digestible particles

[0074] [0074]

Figure CN103891674AD00133

[0075] 表5~7的测定指标与方法 [0075] Table index and measurement method 5-7

[0076] ①日增重(ADG):于正式试验开始前、结束,分别逐栏统一称重并记录,计算每栏增重与各组平均增重。 [0076] ① daily gain (ADG): before the official start of the trial ended, respectively, column by column unified weighed and calculated for each column weight gain and average weight of each group. ②日均采食量(ADFI):每天统计各组猪耗料量,计算每只日平均采食量。 ② daily feed intake (ADFI): Statistics daily pig feed intake of each group, to calculate the average feed intake per day. 采食量=[投给料量(kg)_剩余料量(kg)]/试验期(天数)。 Intake = [administered to the quantity (kg) _ residual quantity (kg)] (days) / test period. ③腹泻率:每天记录猪只腹泻头数,统计腹泻开始时间、程度和发生率。 ③ diarrhea rate: the number of head record daily pig diarrhea, diarrhea statistics start time, and incidence. 腹泻程度按4级评分:粪便半稀、浆糊状、稀粥状未见不消化颗粒、水样状见不消化颗粒,计算腹泻率。 Level 4 according to the degree of diarrhea Rating: semi-dilute manure, paste-like, indigestible no gruel-like particles, see indigestible watery shaped particles, calculating the rate of diarrhea. ④粪便:观察猪只粪便颜色(A灰色、B深色、C灰黑色、D黑色)粪便中不消化颗粒数量及颗粒大小(A可见,较大、B可见,明显、C可见,较小、D细腻、不明显)并进行对比。 ④ stool: stool color was observed pigs (A Gray, B dark, C gray and black, D black) and the number of feces nondigestible particles the particle size (A visible, large, B can be seen clearly, C can be seen, the smaller, D delicate obvious) and compared. ⑤猪皮肤及被毛状况:每日观察猪皮肤颜色(A微红、B红润无红斑、C、苍白),被毛长度、光泽(A—般、B较好)。 ⑤ pig skin and coat conditions: observed daily porcine skin color (A reddish, B red no erythema, C, pale), length of hair, the gloss (as A-, B preferred).

[0077] 试验结果分析 [0077] Test Result

[0078] 乳仔猪阶段:由表5和表6数据整体来看,各组乳仔猪头日增重、腹泻率及料肉比均差异显著(P <0.05)。 [0078] Stage piglets: the data in Tables 5 and 6 as a whole, the head of each group of piglets daily gain, feed conversion rate and diarrhea were significantly different (P <0.05). 试验结论有两点:①处理2组(无抗生素,微量元素与中草药发酵组)与处理I组(高铜+高锌+高抗生素)对比观察其对乳仔猪生产性能的影响,由试验数据可以看出,处理2组的平均日增重较处理I组高5.36%、腹泻率、料肉比分别比处理I组低38.4%,5.52%,差异显著,说明处理2组可完全替代高铜、高锌及高抗生素,且能够提高仔猪生产性能,降低腹泻率和料肉比。 Conclusion There are two tests: ① 2 treated group (without antibiotics, trace elements, herbs and fermentation group) and treatment group I (high Cu + and Zn + high antibiotic) Comparative observe its effect on milk production performance in pigs, experimental data may be made seen, the process ADG 2 treated group than 5.36% higher in group I, diarrhea rate, feed conversion ratio of treatment group I low 38.4%, 5.52%, a significant difference, the process of the two groups can completely replace high copper, high zinc and high antibiotic, and can improve growth performance, feed conversion and reduced diarrhea rate. ②从乳仔猪试验设计表和试验数据表中可以很直观的看出处理2组的微量元素用量明显低于处理I组,且生长性能的各项指标均优于处理I组,元素用量分别是铜比处理I组降低80%以上、铁比处理I组降低45%以上、锌比处理I组降低50-95%,充分体现中草药与微量元素结合后,提高微量元素的利用率,减少排放污染,且大大的节约了矿物资源。 ② piglets from the experimental design and experimental table data table can directly see 2 treated group was significantly lower than the amount of trace elements I treated group, and the growth performance indicators are better than I treated group, the amount of each element is copper I treated group decreased more than 80% than iron lower than that in group I treated more than 45%, 50-95% zinc reduction ratio I treated group, which fully reflects the combined herbal and trace elements, trace elements to improve efficiency and reduce exhaust emissions and greatly saves the mineral resources.

[0079]日常观察结果:处理I组粪便为亮色黑便,明显可见未消化饲料颗粒;处理2组仔猪粪便为深色便,细腻,未消化颗粒相对少,颗粒小。 [0079] Daily observations: I treatment of fecal melena bright, visible undigested feed particles; treatment group 2 as a dark piglet will, fine, undigested particles are relatively few small particles. 处理I组皮肤微红,光泽度一般;处理2组猪皮肤红润(无红斑),光泽度较好。 Reddish skin treatment I group, generally gloss; treated pig skin red 2 groups (no erythema), good gloss.

[0080] 中大猪阶段:由表7数据整体来看,各组采食量、日增重及料肉比均差异明显,处理2组的日增重、采食量分别比处理I组高4.3 %、9.5%,料肉比比处理I低4.87%,差异显著,充分体现处理2组的促生长优势。 [0080] Large pig stages: data from Table 7 Overall feed intake of each group, average daily gain and feed conversion ratio were significantly different, the two groups treated daily gain, feed intake were higher than Group I treated 4.3%, 9.5% lower feed conversion ratio of 4.87% I treatment, a significant difference, a growth advantage to fully reflect promoting treatment group 2.

[0081] 经日常观察结果,处理I组为黑色粪便,均可见未消化饲料颗粒,皮肤微红,光泽较差;处理2组呈深色粪便,细腻成形,未消化饲料颗粒小,毛色光泽度较好组皮肤红润。 [0081] Daily observations by treatment group I black stool, undigested feed particles are visible, reddish skin, poor gloss; treatment group 2 as a dark feces, forming fine, small particles of undigested feed, coat gloss better group ruddy skin.

[0082] 全程料肉比:处理I组料肉比均值为2.83,处理2的料肉比为2.70,处理2全程可降低料肉比0.13。 [0082] full FCR: I treated group mean was 2.83 feed conversion, feed conversion processing 2 is 2.70, the whole process can lower feed 2 0.13. 出栏猪按100公斤核算,可提高经济效益(节省饲料:100公斤*(2.83-2.70) = 13公斤饲料,可增加猪重13/2.70 = 4.81公斤,每公斤生猪按12元计算,可多收益4.81*12 = 57.72元)57.72元。 Accounting slaughter pigs per 100 kg, can improve economic efficiency (feed savings: 100 kg * (2.83-2.70) = 13 kg feed, increased weight of the pig 13 / 2.70 = 4.81 kg, calculated per kg pig $ 12, can be more revenue 12 * 4.81 = 57.72 yuan) 57.72 yuan. 对于一个万头猪场来说,经济效益是非常可观的。 For a pig farms, the economic efficiency is very impressive.

[0083] 综上所述:处理2组的无抗生素、低微量的中草药发酵组可完全替代高铜、高锌、高抗生素的处理I组,在降低微量元素的同时且具有明显的抗病促生长功效,充分体现其能节约资源,降低排放,减轻水土污染,提高经济效益和产品质量,保证食品安全等优势。 [0083] In summary: antibiotic treatment group 2, the lower trace component can completely replace the fermented Chinese herbal high copper, zinc, Group I high antibiotic treatment, while reducing trace element and has a significant antiviral pro growth effect, it can fully reflect conserve resources, reduce emissions, reduce water and soil pollution, increase economic efficiency and product quality, ensure food safety and other advantages.

[0084] 根据上述实验案例,我们以其中的铜元素为例进行计算,我国每年加工的各类饲料总量约1.5亿吨,折合金属铜源约2万吨。 [0084] According to the test case, we calculate an example wherein copper, various types of feed amount of about 150 million tons per year processed, equivalent to about 20,000 tons of metallic copper source. 目前市场主要使用硫酸铜,约占95%以上,有机铜约占不足5 %。 Currently the market is mainly used copper sulfate, accounting for more than 95% organic copper accounts for less than 5%. 如果全部使用技术项目,每年可为国家节约铜源约在1.6万吨以上,降低铜排放量1.6万吨以上,与之相对应的锌和铁的排放也是以万吨计的。 If all the items used techniques, annual savings for the country copper source above about 16,000 tons of copper to reduce emissions by more than 16,000 tons, corresponding to the emission of zinc and iron are of thousands of tons. 每吨铜源按5万元计算,节约资源成本计8亿元人民币、减轻水土污染的成本远远不止是这个数字,这只是仅仅铜元素一项而已。 Per ton of copper 50,000 yuan calculated according to the source, the cost of saving resources at cost 800 million yuan, reduce water and soil pollution is far more than this figure, this is only a copper only. 故本发明所述方法具有重要的市场应用价值和前景。 Therefore, the method of the present invention has important application value and market prospect.

[0085] 实施例4 [0085] Example 4

[0086] 现行农业标准NY/T843-2008绿色食品肉及肉制品规定砷≤0.05mg/kg、铅,(0.lmg/kg、镉< 0.lmg/kg、铜< 10mg/kg、六六六< 0.05mg/kg、滴滴涕< 0.05mg/kg、敌敌畏< 0.02mg/kg、喹乙醇,不得检出(< 0.05mg/kg)、金霉素,不得检出(< 0.lmg/kg)、氯霉素,不得检出(< 0.01mg/kg)、盐酸克伦特罗,不得检出(< 0.002mg/kg)、菌落总数(500000CFU/g、大肠菌群≤ 1000MPN/100g。 [0086] Current Agricultural Standard NY / T843-2008 green meat and meat predetermined arsenic ≤0.05mg / kg, lead, (0.lmg / kg, Cd <0.lmg / kg, Cu <10mg / kg, sixty-six six <0.05mg / kg, DDT <0.05mg / kg, dichlorvos <0.02mg / kg, olaquindox, not detected (<0.05mg / kg), chlortetracycline, not detected (<0.lmg / kg) , chloramphenicol, not detected (<0.01mg / kg), clenbuterol, not detected (<0.002mg / kg), the total number of colonies (500000CFU / g, coliform ≤ 1000MPN / 100g.

[0087] 通过本发明所述方法饲养出来的商品猪,肉质经过谱尼测试检测的结果如下:猪肉中,砷未检出(< 0.01mg/kg)、铅未检出(< 0.01mg/kg)、镉未检出(< 0.01mg/kg)、铜未检出(< 0.lmg*kg)、猪肝中,砷未检出(< 0.0 lmg/kg)、铅< 0.052mg/kgj||< 0.032mg/kg、铜<9.53mg/kg,现在养猪业中肝脏中铜元素残留一般是肉中的几倍,以致于现在的人们根本不敢吃猪肝,但我们的产品实际检测结果却比肉中残留还要低,可见降低饲料中铜元素的用量即可达到减少畜产品的有害残留,人们可以放心的食用。 [0087] By feeding out the method of the present invention, pigs, meat through the detection results of the test are as follows Pony: pork, arsenic not detected (<0.01mg / kg), lead undetectable (<0.01mg / kg ), cadmium was not detected (<0.01mg / kg), copper undetectable (<0.lmg * kg), porcine liver, arsenic not detected (<0.0 lmg / kg), lead <0.052mg / kgj | | <0.032mg / kg, copper <9.53mg / kg, now in the pig industry in the liver copper residues in meat is generally several times, so that now people did not dare to eat liver, but actual testing of our products the result is even lower than meat residues, reducing the visible copper in the feed can be achieved by reducing the amount of harmful residues in animal products, people can rest assured that food. 六六六未检出(< 0.01mg/kg)、滴滴涕未检出(< 0.01mg/kg)、敌敌畏未检出(< 0.01mg/kg)、喹乙醇未检出(< 0.04mg/kg)、金霉素未检出(< 0.005mg/kg)、氯霉素未检出(< 0.0OOlmg/kg)、盐酸克伦特罗未检出(< 0.00025mg/kg)、菌落总数≤9200CFU/g,远低于规定中的500000CFU/g、大肠菌群≤30MPN/100g,远低于规定中的1000MPN/100,符合绿色食品要求。 BHC not detected (<0.01mg / kg), DDT was not detected (<0.01mg / kg), dichlorvos not detected (<0.01mg / kg), olaquindox not detected (<0.04mg / kg) , chlortetracycline not detected (<0.005mg / kg), chloramphenicol not detected (<0.0OOlmg / kg), clenbuterol not detected (<0.00025mg / kg), the total number of colonies ≤9200CFU / g, far below the predetermined 500000CFU / g, coliform ≤30MPN / 100g, far below the predetermined 1000MPN / 100, with green food requirements. 所以本养殖方法生产出来的畜产品做到了安全、绿色、健康、环保,是现在人们追求健康生活的有利保证。 Therefore, this method produced by livestock farming do security, green, health, environmental protection, it is advantageous to ensure that people now pursue a healthy life.

[0088] 本发明不局限于上述最佳实施方式,任何人在本发明的启示下所作的有关本发明的任何修饰或变更,凡是具有与本申请相同或相近似的技术方案,均落在本发明的保护范围之内。 [0088] The present invention is not limited to the preferred embodiment, any change or modification relating to the present invention by any person in light of the present invention, the present application all have the same or similar technical solution like, all fall present within the scope of the invention.

Claims (10)

  1. 1.一种保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:所述生态养殖方法包括以下步骤: ①有益菌种发酵:包括厌氧微生物的厌氧发酵和有氧微生物的有氧发酵;所述厌氧微生物包括植物乳酸菌和粪肠球菌dhf-5,所述有氧微生物包括白地霉菌、酿酒酵母菌、放线菌dha-8以及枯草芽孢杆菌dhk-7 ; 所述厌氧发酵是将厌氧微生物接种到液体培养基中进行无氧恒温培养,并通过一级扩大培养和二级扩大培养得到厌氧发酵菌液;所述有氧发酵是将有氧微生物接种到液体培养基中进行有氧恒温培养,并通过一级扩大培养和二级扩大培养得到有氧发酵菌液; ②益生菌发酵床的制备:将制备发酵床物料的各种原料混合均匀,得到有机垫料,再将厌氧发酵菌液和有氧发酵菌液接种至有机垫料中,进行堆积固态发酵,3-5天后当固体物料温度达到50°C时,将有机 1. A health-fermented feed with ecological farming probiotics fermentation bed, characterized in that: said method comprises the ecological farming: ① From probiotic fermentation: anaerobic fermentation and aerobic microorganisms including anaerobic microorganisms are fermentation oxygen; anaerobic microbes comprises the Lactobacillus plantarum and Enterococcus faecalis dhf-5, the aerobic microorganisms include fungus Geotrichum, Saccharomyces yeasts, actinomycetes dha-8 and Bacillus subtilis dhk-7; the anaerobic the anaerobic fermentation is inoculated into a liquid medium for anaerobic incubation, culture and expansion by an expansion for two anaerobic fermentation broth obtained; the aerobic fermentation of aerobic microorganisms is inoculated into a liquid culture for group aerobic incubation, culture and expansion by an expansion for two aerobic fermentation broth obtained; probiotic fermentation bed ② preparation of: mixing various raw materials to prepare a fermentation bed material uniformly, to give an organic litter when then the anaerobic fermentation and aerobic fermentation broth was inoculated into broth organic litter, for the accumulation of fermentation, the temperature of 3-5 days when the solid material reaches 50 ° C, and the organic 料均匀铺在所述发酵床上,所铺厚度不小于60cm,再对畜禽进行生态养殖;所述有机垫料包括锯末、稻壳、麸皮以及第一复方中草药,所述第一复方中草药包括蒲公英、鱼腥草、茵陈、秦皮、大青叶、淫羊藿、青蒿、穿心莲、龙葵、博落回、地锦草、贯众与土荆皮; ③发酵饲料的成份:a.教槽保育饲料包括玉米50kg、豆柏35kg和小料15kg,其中小料包括维生素0.2kg、微量元素0.06kg、矿物质2.91kg、氨基酸1.2kg、其它添加剂9.13kg以及第二复方中草药1.5kg ;b.仔猪饲料包括玉米55kg、豆柏22kg、花生柏5kg、麸皮IOkg以及小料8kg,其中小料包括维生素0.2kg、微量元素0.05kg、矿物质2.91kg、氨基酸1kg、其它添加剂2.34kg以及第二复方中草药1.5kg ;c.中大猪饲料包括玉米55kg、豆柏12kg、棉柏4kg、麸皮15kg以及小料14kg ;其中小料包括维生素0.2kg、微量元素0.04kg、矿物质2.44kg、氨基酸0.8 Was uniformly spread in the fermentation bed, the lay thickness of not less than 60cm, and then the livestock ecological farming; the organic gasket comprises sawdust, rice hulls, bran, and a first herbal compound, said first compound Chinese herbal medicine comprises dandelion, Houttuynia, wormwood, ash bark, Folium, Epimedium, Artemisia annua, Andrographis, Solanum nigrum, Macleaya, humifusa, Guanzhong and pseudolaric; ③ fermentation of feed ingredients: a. conservation creep feed include corn 50kg, 35kg and small material beans Bo 15kg, wherein the feed comprises small vitamins 0.2kg, trace elements 0.06kg, minerals 2.91kg, amino 1.2kg, other additives 9.13kg 1.5kg and a second compound Chinese herbal medicine; b. piglet feed include corn 55kg, 22kg Bo beans, peanuts Bo 5kg, bran and small material IOkg 8kg, wherein the feed comprises small vitamins 0.2kg, trace elements 0.05kg, minerals 2.91kg, amino 1kg, and other additives 2.34kg The second compound Chinese herbal medicine 1.5kg; c large swine feed include corn 55kg, 12kg beans Bo, Bo cotton 4kg, 15kg bran and small material 14kg;. wherein small materials include vitamins 0.2kg, trace elements 0.04kg, 2.44kg minerals , amino acids 0.8 kg、其它添加剂9.02kg以及第二复方中草药1.5kg ;所述第二复方中草药包括杜仲、蒲公英、鱼腥草、茵陈、秦皮、大青叶、淫羊藿、青蒿、穿心莲、龙葵、地锦草、山楂、麦芽以及神曲; ④发酵饲料的制备:a.菌种培育:按步骤①中所述厌氧发酵的方法来制得植物乳酸菌和粪肠球菌dhf-5的厌氧发酵的菌液;按步骤①中所述有氧发酵方法来制得酿酒酵母菌的有氧发酵菌液;b.将步骤③中所述的饲料中除小料外的其它饲料成份投入到卧式搅拌机中,边搅拌边蒸煮至80°C进行高温灭菌熟化,然后冷却至40°C以下后加入小料,均匀混合,并将加入了5%糖蜜的厌氧发酵菌液和酿酒酵母菌的有氧发酵菌液混合均匀,再接种至加入了小料的饲料中,堆积厌氧发酵3-5天,当pH < 4.5,停止发酵,得到发酵饲料。 kg, and other additives 9.02kg 1.5kg second compound Chinese herbal medicine; chinese herbal medicine comprising the second eucommia, dandelion, Houttuynia, capillaris, Qinpi, Folium, Epimedium, Artemisia annua, Andrographis, Solanum nigrum, Humifusae, hawthorn, malt and Shinkyoku; ④ preparation of fermented feed: a cultivate bacteria: the method of anaerobic fermentation in step ① be prepared by lactobacillus plantarum and Enterococcus faecalis dhf-5 anaerobic fermentation. bacteria; step ① by the aerobic fermentation process in aerobic fermentation broth obtained from the system of Saccharomyces cerevisiae; b. the step ③ said other feed ingredients in feed materials into small addition to the horizontal mixer. , the small material, uniformly mixed with stirring cooked to 80 ° C for high-temperature sterilization aged, then cooled to below 40 ° C was added, and the anaerobic fermentation of the bacterial suspension was added 5% molasses and Saccharomyces cerevisiae have oxygen mixed fermentation broth, then added to the inoculated into small material feed, bulk 3-5 days anaerobic fermentation, when pH <4.5, the fermentation was stopped to give fermentation feed.
  2. 2.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤①中所述厌氧发酵的具体过程是将保存的植物乳酸菌、粪肠球菌dhf-5接种到液体培养基中,在无氧条件下37°C恒温培养24h,当发酵液pH < 4.5时,将其移种至50L的发酵罐中进行一级扩大培养,在无氧条件下37°C恒温培养24h,当发酵罐内的发酵液pH< 4.5时,将其移种至1000L的发酵罐中进行二级扩大培养,无氧条件下37°C恒温培养30h,当发酵罐内的发酵液PH < 4.5时,结束发酵,得到所述的厌氧发酵菌液。 The health of the fermented feed as claimed in claim 1, with the ecological farming probiotics fermentation bed, characterized in that: ① in the specific process step is to save the anaerobic fermentation of L. plantarum, Enterococcus faecalis dhf-5 inoculated into a liquid medium, under anaerobic conditions at 37 ° C incubated 24h, when the fermentation broth pH <4.5, it will be moved to perform a seed culture expansion in 50L fermentor at 37 ° under anaerobic conditions C incubation 24h, for secondary expansion when fermentation culture a fermentor, pH <4.5, it is transplanted to the 1000L fermenter, at 37 ° C anaerobically incubated 30h, when a fermentation tank liquid PH <4.5, the end of fermentation, anaerobic fermentation broth obtained according to.
  3. 3.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤①中所述的有氧发酵的具体过程是将所述有氧微生物接种到液体培养基中,有氧条件下30°C恒温培养24h,当发酵液pH < 5.5时,将其移种至50L的发酵罐中进行一级扩大培养,在有氧条件下30°C恒温培养24h,当罐内发酵液pH < 5.5时,将其移种至1000L的发酵罐中进行二级扩大培养,在有氧条件下30°C恒温培养30h,当发酵罐内发酵液pH <5.5时,结束发酵,得到所述的有氧发酵菌液。 The health of the fermented feed as claimed in claim 1, with the ecological farming probiotics fermentation bed, characterized in that: the specific process steps ① aerobic fermentation according to the aerobic microorganism is inoculated into a liquid medium in aerobic conditions at 30 ° C incubation 24h, for expanding a culture broth when pH <5.5 when it is transplanted to the 50L fermentor, under aerobic conditions in a 30 ° C incubation 24h, when tank fermentation broth at pH <5.5, for which two expanded transplanting cultured in a fermentor 1000L, under aerobic conditions in a 30 ° C incubation 30h, when the fermenter broth pH <5.5, the end of fermentation , aerobic fermentation broth obtained according to.
  4. 4.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤②中所述有机垫料的成份及其重量份数包括40%的锯末、40%的稻壳、5%的麸皮以及15%的第一复方中草药,所述第一复方中草药中各种成份的重量份数为:蒲公英1-2份、鱼腥草1-2份、茵陈1-2份、秦皮1-2份、大青叶1-2份、淫羊藿1-2份、青蒿1_2份、穿心莲1-2份、龙葵1-2份、博落回2-4份、地锦草1-2份、贯众1-3份与土荆皮1_4份。 The health of the fermented feed as claimed in claim 1, with the ecological farming probiotics fermentation bed, characterized in that: in the step ② gasket organic ingredients and comprising 40% by weight of sawdust, 40% rice husks, 5% of wheat bran and 15% of the first compound Chinese herbal medicine, said first compound Chinese herbal medicine in parts by weight of various components is: dandelion parts 1-2, 1-2 parts of Houttuynia capillaris 1 2 copies, ash bark 1-2 parts, Folium 1-2 parts, 1-2 parts of epimedium, Artemisia annua 1_2 parts of Andrographis 1-2 parts, 1-2 parts of Solanum nigrum, Macleaya 2-4 parts, parts Humifusa 1-2, 1-3 parts and rhizome pseudolaric 1_4 parts.
  5. 5.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤②中所述的厌氧发酵菌液和有氧发酵菌液是以1:1的体积比混合均匀,再按10%的接种量将其接种至有机垫料中。 The health of the fermented feed as claimed in claim 1, with the ecological farming probiotics fermentation bed, characterized in that: in the step ② of anaerobic fermentation and aerobic bacteria fermentation broth is 1: 1 by volume uniformly mixing ratio, then 10% of the inoculum was inoculated into the organic litter.
  6. 6.根据权利要求5所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:接种后的所述有机垫料的含水量不超过60%。 According to claim 5, wherein the fermented feed health complex ecological farming probiotics fermentation bed, characterized in that: the water content of the organic inoculated dunnage does not exceed 60%.
  7. 7.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤②中所述的发酵床的大小为不小于20m2,所述畜禽为猪,平均每头猪所占面积为1.0 ~1.5m2。 The health of the fermented feed as claimed in claim 1, with the ecological farming probiotics fermentation bed, characterized in that: the step size ② of the fermentation bed is not less than 20m2, the livestock is a pig, the average pigs occupied an area of ​​1.0 ~ 1.5m2.
  8. 8.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤③中所述的教槽保育饲料中的微量元素的成份及其含量分别为:碱铜6.7克、硫酸亚铁33.4克、硫酸锰9.5克、氧化锌11.7克、碘化钾0.046克以及亚硒酸钠0.068克;所述的仔猪饲料中的微量元素的成份及其含量分别为:碱铜5.17克、硫酸亚铁26.7克、硫酸锰9.5克、氧化锌9.3克、碘化钾0.046克以及亚硒酸钠0.068克;所述的中大猪饲料中的微量元素的成份及其含量分别为:碱铜3.88克、硫酸亚铁20克、硫酸锰9.5克、氧化锌7克、碘化钾0.046克以及亚硒酸钠0.068克。 Health fermented feed according to claim 1, said complex ecological farming probiotics fermentation bed, characterized in that: the content of trace elements and components in the step ③ said creep feed conservation are: base copper 6.7 grams, 33.4 grams ferrous sulfate, manganese sulfate 9.5 g, 11.7 g of zinc oxide, 0.046 g of potassium iodide and sodium selenite 0.068 g; of the trace elements in piglet feed ingredients and contents were: base copper 5.17 grams, 26.7 grams ferrous sulfate, manganese sulfate 9.5 g, 9.3 g of zinc oxide, 0.046 g of potassium iodide and 0.068 g of sodium selenite; large pig feed the ingredients in trace elements and contents were: base copper 3.88 grams, 20 grams ferrous sulfate, manganese sulfate 9.5 g, 7 g of zinc oxide, 0.046 g of potassium iodide and 0.068 g of sodium selenite.
  9. 9.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤③中所述的第二复方中草药中各种成份及其重量份数为杜仲10-75份、蒲公英1-20份、鱼腥草1-30份、茵陈1-30份、秦皮1-30份、大青叶1-30份、淫羊藿1-30份、青蒿10-60份、穿心莲1-30份、龙葵10-50份、地锦草1-30份、山楂10-50份、麦芽10-50份、神曲10-50份。 9. The health fermented feed according to claim 1 with ecological farming probiotics fermentation bed, characterized in that: in the step ③ said second compound Chinese herbal medicine and the various components of 10-75 parts by weight of eucommia copies, 1-20 parts dandelion, Houttuynia 1 to 30 parts, 30 parts wormwood, ash bark 1-30 parts, Folium 1-30 parts, epimedium 1-30 parts, Artemisia annua 10-60 parts of Andrographis 1-30 parts, Solanum nigrum 10-50 parts, humifusa 1-30 parts, hawthorn 10-50 parts, 10-50 parts malt, Divine Comedy 10-50 parts.
  10. 10.根据权利要求1所述的保健发酵饲料配合益生菌发酵床的生态养殖方法,其特征在于:步骤④中所述的发酵饲料制备的具体过程为:a.将除小料外的其它饲料成份投入到卧式搅拌机中,边搅拌边蒸煮至温度80°C进行高温灭菌熟化,保持20分钟;再用提升机输送到旋转式搅拌机,冷却,待温度降低至40°C以下后,在其中均匀加入小料,与灭菌后的其它饲料成份均匀混合4~7分钟;b.向以体积比1:1混合后的步骤③所述的厌氧发酵菌液和酿酒酵母菌的有氧发酵菌液中加入5%的糖蜜,混合均匀得接种物;c.将步骤b中所述的接种物按10%的接种量接种至步骤a中混合了小料的饲料中,接种后的饲料的含水量为40~50%,堆积厌氧发酵3-5天,发酵至pH < 4.5时,停止发酵,得到发酵饲料。 10. The health-fermented feed according to claim 1 with ecological farming probiotics fermentation bed, characterized in that: the specific process of preparing fermented feed in the step ④ of:. A small addition to the other feed material ingredients into a horizontal mixer with stirring to a temperature of 80 ° C cooking temperature sterilization for curing, 20 min; then lift the conveyor to rotary agitator, cooling until the temperature drops to below 40 ° C, in wherein the homogeneous material by adding a small, uniform mixing with other feed components after sterilization 4 to 7 minutes; to B volume ratio of 1: 1 the yeast mixing step ③ said anaerobic fermentation aerobic bacteria and Saccharomyces. fermentation broth added 5% molasses, uniformly mixed to obtain inoculum;. c in step b the step of inoculum to feed a small mixing 10% of the inoculum in the feed, the feed after vaccination moisture content of 40 to 50% and the bulk 3-5 days anaerobic fermentation, the fermentation to pH <4.5, the fermentation was stopped to give fermentation feed.
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