CN103891674A - Ecological cultivation method with health care fermented feed matched with probiotic fermentation bed - Google Patents
Ecological cultivation method with health care fermented feed matched with probiotic fermentation bed Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K31/00—Housing birds
- A01K31/04—Dropping-boards; Devices for removing excrement
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to an ecological cultivation method with health care fermented feed matched with a probiotic fermentation bed. The method includes the steps that first, probiotic fermentation is carried out, wherein the probiotic fermentation comprises anaerobic fermentation of anaerobes and aerobic fermentation of aerobic microorganisms; second, the probiotic fermentation bed is prepared, wherein various raw materials for preparing the material of the fermentation bed are evenly mixed so as to obtain organic bedding, bacterium solution is inoculated for fermentation, and the organic bedding contains saw dust, rice husks, bran and first compound Chinese herbal medicine; third, the fermented feed contains corn, bean pulp, vitamins, microelements, mineral substances, amino acids, other additives, second compound Chinese herbal medicine and the like, and microorganisms are added into the feed components for fermentation. The ecological cultivation method has the advantages that water can be purified, and hidden danger to health of hogs caused by a water source is blocked; the using amount of the microelements is reduced, resources are saved, emissions are reduced, and the environment is protected; no antibiotics or residue exists, and food safety and human health are guaranteed; the Chinese herbal medicines have the functions of being resistant to disease and promoting growth and can replace antibiotics.
Description
Technical field
The present invention relates to biofermentation technique in agriculture field and is applied to trace element and field of Chinese herbal, and is applied in feedstuff industry, is specifically related to the ecological cultivation method that a kind of fermented forage that keeps healthy coordinates probiotics fermention bed.
Background technology
Along with aquaculture scale, business-like development, the producer is devoted to the raising of breeding performonce fo animals and quantity always in recent years, but does not cause enough attention on animal food safety and problem of environmental pollution.So that food security and Environment Pollution Event constantly occur.Though extremely country payes attention to, and under the ordering about of interests factor, is not effectively improved.Therefore, consequently increasing people thirst for country can strengthen the monitoring to food security and environmental pollution, guarantees that people can have the food of the relieved health of safety, and current country has formulated a series of laws and regulations, but this all can not play the effect of effective containment.China's intensive culture causes at present environmental pollution and animal product residue problem are more serious.Be mainly that the trace element of replenishing in feed is mostly inorganic salt form, because animal body is poor to the mineral element abilities of digestive and absorption of inorganic states, availability is low, so must excess add the due effect of just playing.This is one, and it two is in Swine Production, generally to adopt high-copper (copper sulphate), high zinc (zinc oxide) and generally grain of high antibiosis, to improve the productivity of animal.In causing data waste, environmental pollution, affect quality and the human health of animal product.
Barber etc. (1995) find to add the copper of 10 times of animal normal needs first in feed, can obviously improve its growth rate and food conversion.People also find that the zinc oxide of high dose can improve daily ingestion amount and daily gain reduction feedstuff-meat ratio and the grice diarrhoea rate of piglet, Feedstuff Enterprises and raiser generally add the zinc oxide high dose trace element that 2000-3000mg/kg is even higher (high-copper and high zinc) as effective growth-promoting additive in piglet feed, have become pig industry nutrition regulation means comparatively widely.
In Swine Production, high-copper uses quite general as growth-promoting additive, due to the antagonism between trace element, has also improved the consumption of the trace element such as zinc, iron simultaneously.In pig feed, use the inorganic microelements such as a large amount of copper (Cu), zinc (Zn), iron (Fe) because absorption rate is low, trace element is discharged in a large number through ight soil, and environment (soil, water system etc.) is caused to severe contamination.In view of high dose trace element adds the harm causing, the European Community has proposed the sanitary standard of Feed Copper allowance.Stipulate piggy 175mg/kg in 16 weeks, 17 weeks above is 35mg/kg, and Canada, U.S. feed association have been 125mg/kg and 500mg/kg by copper in daily ration and the maximum limitation of zinc separate provision.China also adjusts micro-usage amount in feed, piggy 200mg/kg, and growing and fattening pigs are 35mg/kg.High-copper not only can cause pig copper poisoning, also causes that some nutrient (as VA, VD, VE, VB2) of pig lacks, and when Feed Copper content is during up to 250ppm, meat quality is had to bad shadow, thereby cause food-safety problem.Holland considers environmental protection, no longer allows to use high-copper and high zinc as growth accelerator in daily ration: Japan also strictly limits the consumption of copper, zinc.
In Modern Animal Husbandry is produced the pollution of environment, the trace elements such as copper, zinc account for the overwhelming majority.This not only causes animal micro-element nutrition surplus and waste economically, and ecotope is caused to severe contamination.It is reported, the annual microelement additive using of China is 15～180,000 tons, but because its availability is low, nearly 100,000 tons of left and right are not discharged contaminated environment by the mineral matter of animal use with fecaluria becomes large public hazards.In general feeds inorganic microelement as the addition of copper be 20～40 times of body normal needs, after Feed Copper metabolism, 90% arranges through ight soil, copper is non-degradable material, will inevitably cause soil, water source, vegetation seriously dirty, hinders the development of environmental protection type agricultural.Zinc major part is from unabsorbed external source zinc in daily ration, and the research Pharmacological Level of Zinc daily ration group pig zinc every day discharge rate that shows to feed is 33 times of basic ration group of feeding, and so the zinc of high dose enters in environment through defecate, makes zinc in soil accumulation and contaminated environment.If infer that according to Japanese relevant expert the zinc in all pig manures rests in soil, the zinc content contaminated soil of 120mg/kg that will exceed standard in 17 years will no longer have arability, and zinc also can polluted underground water after carcass excretion hinders the sustainable development of livestock breeding to fish and the larger fishery water quality requirement of hydrobiological toxicity zinc excessive zinc within 0.1mg/L
So the micro-consumption of high dose, will certainly affect animal product quality, copper tolerance limit (GB15199-94) regulation in China's meat product, and in pork, copper content is no more than 10ppm.High-copper daily ration can cause pork liver copper content to increase 10-15 doubly, people can cause copper accumulation in the tissues such as liver, brain and kidney after eating the animal product that cupric is high, the level of increase machine interior free yl, changes lipid metabolism, causes atherosclerotic and accelerates the aging and dead of cell.
From 1950's, animal scientists find, in feed, the antibiotic of low concentration can promote growth of animals or poultry significantly, and fodder antibiotics just widely uses, in the world each feed production state almost none does not add various antibiotic in feed.Since should saying nearly half a century, fodder antibiotics, to prevention Animal diseases, promotes growth of animal, improves the price of deed and has really played positive effect.There are in the world according to statistics 20 Multiple Classes of Antibiotics and tens kinds of synthetic antimicrobial class medicines to be applied in feed.Be that antibiotic should period with the most use by feedstuff industry the sixties to the beginning of the eighties.
As everyone knows, antibiotic has been made huge contribution as producing promoter to the development of livestock breeding.But, the caused medicament residue of antibiotic use lack of standardization, the problems such as drug resistance attract great attention.For many years, due to the long-term a large amount of antibiotic that use of China raiser, livestock and poultry profitable strain is killed, causes body flora imbalance, suprainfection, drug resistance etc., immunologic function of livestock and birds and premunition, productivity decline.Aquaculture cost increases.Moreover, in years, the expert of Europe and the U.S. investigates at this point in the past, and conclusion is that bacterium may be transferred to people to the tolerance of medicine in animal body.Drug resistance even can pass to human body by animal products, brings threat to the mankind's health, treatment.According to Chinese animal doctor's magazine (1992), " rabid ox disease " of making us in recent years terrified is also to cause to the three-in-one injection of one that under beef injection antibiotic, hormone and sheep horse, water is made due to long-term, and for example the generation of " pseudomembrane intestines claim again antibiotic enteritis " also has substantial connection with abuse of antibiotics.
Antibiotic use not only suppresses or has killed pathogenic microorganism, also suppress simultaneously or killed the beneficial bacterium in animal body, break microecological balance, cause some hypovitaminosis of animal, immunocompetence reduces, even make non-sensitive flora and fungi amount reproduction, cause the obstacle of digesting and assimilating so that secondary infection causes animal morbidity or dead.As long-term use antibiotic, make livestock and poultry body produce dependence, limit the performance of immunocyte function in body, body's immunity is declined, resist the disease ability reduces.Antibiotic after animal alimentary canal plays a role, enters the blood circulation of animal with feed, excretes, but still have some in animal body residual through the filtration great majority of kidney with urine.These residual antibiotic are accumulated in animal product as in meat, egg, milk, hair, are directly threatening health.Use antibiotic likely to make animal produce gene mutation, deformity, carcinogenic; Antibiotic is discharged outside animal body in a large number, also may cause environmental pollution.According to reports indicate that, in feed, use for a long time the antibiotic of low dosage, can increase the probability that drug resistance produces.
In view of the serious side effect of antibiotic, EU Committee's legislation forbade adding bacitracin, spiramycin, virginiamycin, 4 kinds of antibiotic of tylosin feed from 1 day July in 1999.And be defined in the tissue of animal and must can not have detected medicament residue, this just has higher requirement to China's export animal products.At present, U.S. FDA has been cancelled penicillin, tetracycline, erythromycin, lincomycin, tylosin and virginiamycin from agricultural use.
In order to solve the problems such as animal product safety hidden danger, various countries scholar has carried out the research to green feed additive.Now existing probiotics and enzyme preparation, probiotics is by increasing the beneficial bacterium in digestive tract, suppress harmful bacteria, adjust or maintain gut flora balance, reach prevent disease, promote growth, improving the effect of efficiency of feed utilization; In enzyme preparation, contain the multiple enzyme that decomposes nutriment in feed, help animal digestion to absorb, promote growth of animal, improve efficiency of feed utilization.But these products can not reach the effect of fodder antibiotics completely.Chinese herbal medicine is the traditional medicine of preventing and curing diseases of China, and hope is sent in the health care for animal on Chinese herbal medicine by people now.Most research shows, Chinese herbal feed additive has growth of animals or poultry and the health-care effect of promotion, and its toxic and side effect is little, noresidue, the unique advantage such as have no drug resistance, but Chinese medicine compound prescription amount is large, absorption difference, and extract is expensive, effect is single, in application, be restricted.
People obtains very large progress with field of medicaments to Effective Component of Chinese Medicine and micro-coordination research, along with developing rapidly and further investigation to Chinese medicine physiology, pharmacological active substance of bioinorganic chemistry, a large amount of achievements in research using trace element (inorganic constituents) important active ingredient and the significance to life, health and curative effect in Chinese medicine, has given sufficient identification.Micro-research, also by simple mensuration, is progressively goed deep into.Show there is stronger pharmacologically active after trace element coordinates with organic principle in Chinese herbal medicine according to Effective Component of Chinese Medicine and micro-coordination theory research, some is also higher than organic compound for its activity.Pharmacology effectiveness is combining form mostly, easily penetrates in tissue than alone mineral salt.For the domestic research report that there is not yet of the application at feedstuff industry of plant extracts and micro-complex.
Along with the enhancing of global Consciousness of food security, under current cultivating condition, how, in performance animal largest production performance, can guarantee again the health of animal, improving body immunity and the resistance to foeign elements such as diseases etc. more and more becomes the core content of Production of Livestock and Poultry.Coordinate trace element and the Chinese herbal medicine a kind of NEW TYPE OF COMPOSITE livestock and poultry probiotics forming that ferments so our company applies probio by research, in order to substitute antibiotics, reduce micro-consumption simultaneously.This product can, by changing intestinal microflora, overcome the adverse consequences of antibiotic and chemicals, has growth promotion, prevents and cures diseases, the effect such as health care, can significantly improve livestock and poultry production performance, improves efficiency of feed utilization etc.These animal product that can not only tighten security are produced and environmental protection, ensure people's health, can promote again sustainable development of animal husbandry simultaneously, meet the needs of China's Foreign Trade, are also the needs of national politics economic stability.
In the prior art, livestock and poultry cultivation has produced a large amount of ight soil, in feces of livestock and poultry, contain a large amount of nitrogen, phosphorus and organic pollutant etc., can produce afterwards by fermentation the pernicious gases such as a large amount of ammonia nitrogens, hydrogen sulphide, scatol, methane, these gases not only can destroy ecology, but also can directly affect human health.Livestock and poultry farm (family) environmental consciousness is poor in addition, arbitrarily stacks feces of livestock and poultry and sewage effluent, and dirt enters water system with rainwater and sewage, and severe contamination underground water causes soil property and water quality constantly to worsen.The problem of environmental pollution that aquaculture is brought to be to attract great attention, and that conscientiously draw that certain areas exist in animal husbandry development process first pollutes the lesson preventing and treating afterwards.
In addition, since reform and opening-up, China's livestock breeding has obtained significant progress, and the at present production of meat of China leaps to the first in the world, and has become genuine herding big country.But livestock breeding remains low yield industry in China, for improving the productivity of animal, multiplex and abuse feed addictive (copper, zinc, antibiotic and the class additive that promotes production) has become the ubiquitous problem of feedstuff industry.Country has also put into effect some corresponding laws and regulations and has been limited, but it is not fully up to expectations to produce effects.These additive parts remain in the middle of animal product, are eaten the health that directly affects people; The direct entered environment of part follower excreta, causes certain pollution to soil, air, water source, and then enters human body by food chain, same potential threat human health.Therefore, how to solve livestock breeding safety in production and the impact of environment has been become to problem demanding prompt solution in current sustainable development of animal husbandry.Along with China's rapid development of economy, the popularization of the ecological agriculture, feedstuff industry is increasing to the demand of environmentally friendly machine.
Summary of the invention
The object of this invention is to provide the ecological cultivation method that a kind of fermented forage that keeps healthy coordinates probiotics fermention bed; Chinese herbal medicine in the method and trace element are by fermentation; form a kind of new compound; can reduce micro-consumption; and substitute antibiotic completely, economized on resources, reduced discharge; protection of the environment, improves again product quality.And fermentation bed of the present invention makes up existing ecological bed shortcoming by Chinese herbal medicine with ecological bed being combined with, and has solved mould, pathogenic bacteria, the problems such as parasitic generation; And the present invention effectively overcomes food security and problem of environmental pollution that existing feed uses Tong Yuan, high zinc, high antibiotic and growth-promoting class additive to bring.
The object of the invention is to be achieved through the following technical solutions:
The fermented forage that keeps healthy coordinates an ecological cultivation method for probiotics fermention bed, and described ecological cultivation method comprises the following steps:
1. probiotics fermentation: the anaerobic fermentation and the aerobic fermentation that has oxygen animalcule that comprise anaerobe; Described anaerobe comprises plant lactobacillus and enterococcus faecalis dhf-5, described in have oxygen animalcule to comprise Geotrichum, S. cervisiae, actinomycetes dha-8 and bacillus subtilis dhk-7; Described anaerobic fermentation is that anaerobe is inoculated into and in liquid nutrient medium, carries out anaerobic constant temperature culture, and is expanded and cultivated and secondary expands cultivation and obtains anaerobic fermentation bacterium liquid by one-level; Described aerobic fermentation is that aerobic microbial inoculant is carried out to aerobic constant temperature cultivation in liquid nutrient medium, and is expanded and cultivated and secondary expands cultivation and obtains aerobic fermentation bacterium liquid by one-level; 2. the preparation of probiotics fermention bed: the various raw materials of preparation fermentation bed material are mixed, obtain organic bedding and padding, again anaerobic fermentation bacterium liquid and aerobic fermentation bacterium liquid are seeded in organic bedding and padding, pile up solid state fermentation, after 3-5 days in the time that solid material temperature reaches 50 ℃, by organic bedding and padding uniform spreading, in described fermentation bed, the thickness that spreads is not less than 60cm, then livestock and poultry are carried out to ecologic breeding; Described organic bedding and padding comprise sawdust, rice husk, wheat bran and the first compound Chinese herb, and described the first compound Chinese herb comprises dandelion, cordate houttuynia, oriental wormwood, the bark of ash, folium isatidis, barrenwort, sweet wormwood, Herba Andrographitis, black nightshade, macleaya cordata, wolf's milk, rhizome of cyrtomium and Golden Larch Bark; 3. the composition of fermented forage: a. religion groove agent of feed for nursing comprises corn 50kg, dregs of beans 35kg and small powder 15kg, and wherein small powder comprises vitamin 0.2kg, micro-0.06kg, mineral matter 2.91kg, amino acid/11 .2kg, other additive 9.13kg and the second compound Chinese herb 1.5kg; B. pig starter feed comprises corn 55kg, dregs of beans 22kg, peanut meal 5kg, wheat bran 10kg and small powder 8kg, and wherein small powder comprises vitamin 0.2kg, micro-0.05kg, mineral matter 2.91kg, amino acid/11 kg, other additive 2.34kg and the second compound Chinese herb 1.5kg; C. middle hog grower feed comprises corn 55kg, dregs of beans 12kg, cotton dregs 4kg, wheat bran 15kg and small powder 14kg; Wherein small powder comprises vitamin 0.2kg, micro-0.04kg, mineral matter 2.44kg, amino acid 0.8kg, other additive 9.02kg and the second compound Chinese herb 1.5kg; Described the second compound Chinese herb comprises the bark of eucommia, dandelion, cordate houttuynia, oriental wormwood, the bark of ash, folium isatidis, barrenwort, sweet wormwood, Herba Andrographitis, black nightshade, wolf's milk, hawthorn, Fructus Hordei Germinatus and Divine Comedy; 4. the preparation of fermented forage: a. breeding strain: by step 1. described in the method for anaerobic fermentation make the bacterium liquid of the anaerobic fermentation of plant lactobacillus and enterococcus faecalis dhf-5; By step 1. described in aerobic fermentation method make the aerobic fermentation bacterium liquid of S. cervisiae; B. by step, in the feed described in 3., other feed composition except small powder is put in horizontal mixer, high-temperature sterilization slaking is carried out in boiling to 80 while stirring ℃, then after below being cooled to 40 ℃, add small powder, evenly mix, and by having added the anaerobic fermentation bacterium liquid of 5% molasses and the aerobic fermentation bacterium liquid of S. cervisiae to mix, inoculate to having added in the feed of small powder, pile up anaerobic fermentation 3-5 days, as pH < 4.5, stop fermentation, obtain fermented forage.
Further, described other additive comprises the compositions such as acidulant.
Further, step 1. described in the detailed process of anaerobic fermentation be by the plant lactobacillus of preserving, enterococcus faecalis dhf-5 is inoculated in liquid nutrient medium, 37 ℃ of constant temperature culture 24h under oxygen free condition, in the time of zymotic fluid pH < 4.5, its culture transferring is carried out to one-level expansion cultivation to the fermentation tank of 50L, 37 ℃ of constant temperature culture 24h under oxygen free condition, in the time of zymotic fluid pH < 4.5 in fermentation tank, its culture transferring is carried out to secondary expansion cultivation to the fermentation tank of 1000L, the lower 37 ℃ of constant temperature culture 30h of oxygen free condition, in the time of zymotic fluid pH < 4.5 in fermentation tank, finish fermentation, obtain described anaerobic fermentation bacterium liquid.
Further, the detailed process of the aerobic fermentation of step described in be 1. by described aerobic microbial inoculant in liquid nutrient medium, the lower 30 ℃ of constant temperature culture 24h of aerobic conditions, in the time of zymotic fluid pH < 5.5, its culture transferring is carried out to one-level expansion cultivation to the fermentation tank of 50L, 30 ℃ of constant temperature culture 24h under aerobic conditions, when in tank when zymotic fluid pH < 5.5, its culture transferring is carried out to secondary expansion cultivation to the fermentation tank of 1000L, 30 ℃ of constant temperature culture 30h under aerobic conditions, when in fermentation tank when zymotic fluid pH < 5.5, finish fermentation, obtain described aerobic fermentation bacterium liquid.
Further, in described anaerobic fermentation and aerobic fermentation, the composition of liquid nutrient medium used comprises: glucose, sulfate of ammoniac, manganese sulphate, magnesium sulfate, potassium dihydrogen phosphate, corn, dregs of beans and deionized water.
Further, step 2. described in the composition of organic bedding and padding and parts by weight thereof comprise 40% sawdust, 40% rice husk, 5% wheat bran and the first compound Chinese herb of 15%, in described the first compound Chinese herb, the parts by weight of various compositions are: dandelion 1-2 part, cordate houttuynia 1-2 part, oriental wormwood 1-2 part, bark of ash 1-2 part, folium isatidis 1-2 part, barrenwort 1-2 part, sweet wormwood 1-2 part, Herba Andrographitis 1-2 part, black nightshade 1-2 part, macleaya cordata 2-4 part, wolf's milk 1-2 part, rhizome of cyrtomium 1-3 part and Golden Larch Bark 1-4 part.
Further, anaerobic fermentation bacterium liquid and the aerobic fermentation bacterium liquid of step described in is 2. to mix with the volume ratio of 1: 1, then is seeded in organic bedding and padding by the inoculum concentration of 10% (mass percent).
Further, the water content of postvaccinal described organic bedding and padding is no more than 60%.
Further, the size of the fermentation bed of step described in is 2. for being not less than 20m
2, described livestock and poultry are pig, average every pig area occupied is 1.0～1.5m
2.
Further, micro-composition and the content thereof in the religion groove agent of feed for nursing of step described in is 3. respectively: 0.068 gram of 6.7 grams of alkali copper, 33.4 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 11.7 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite; Micro-composition and content thereof in described pig starter feed are respectively: 0.068 gram of 5.17 grams of alkali copper, 26.7 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 9.3 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite; Micro-composition and content thereof in described middle hog grower feed are respectively: 0.068 gram of 3.88 grams of alkali copper, 20 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 7 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite.
Further, in second compound Chinese herb of step described in 3., various compositions and parts by weight thereof are bark of eucommia 10-75 part, dandelion 1-20 part, cordate houttuynia 1-30 part, oriental wormwood 1-30 part, bark of ash 1-30 part, folium isatidis 1-30 part, barrenwort 1-30 part, sweet wormwood 10-60 part, Herba Andrographitis 1-30 part, black nightshade 10-50 part, wolf's milk 1-30 part, hawthorn 10-50 part, Fructus Hordei Germinatus 10-50 part, Divine Comedy 10-50 part.
Further, the detailed process that prepared by the fermented forage of step described in is 4.: a. puts into other feed composition except small powder in horizontal mixer, and boiling while stirring to 80 ℃ of temperature are carried out high-temperature sterilization slaking, keeps about 20 minutes; Be transported to rotary agitator with elevator again, cooling, after temperature is reduced to below 40 ℃, evenly add therein small powder, mix 4～7 minutes preferably 5 minutes with other feed uniform ingredients after sterilizing; B. to the molasses that 3. add 5% (referring to percentage by volume) with 1: 1 mixed step of volume ratio in the aerobic fermentation bacterium liquid of described anaerobic fermentation bacterium liquid and S. cervisiae, mix to obtain inoculum; C. the inoculum described in step b is seeded to by the inoculum concentration of 10% (referring to mass percent) in the feed that has mixed small powder in step a, pile up anaerobic fermentation 3-5 days, while fermenting to pH < 4.5, stop fermentation, obtain fermented forage, i.e. fermentation stops fermentation when having wine flavour, just can be used for the livestock and poultry of feeding.
Further, the water content of postvaccinal feed is 40～50%, and preferred water content is 45%.
The invention provides the ecological cultivation method that a kind of fermented forage that keeps healthy coordinates probiotics fermention bed, key technology point of the present invention is by organic bedding and padding such as Chinese herbal medicine are fermented, and be added in fermentation bed livestock and poultry are cultivated, and feed aspect, to comprise that Chinese herbal medicine ferments together with trace element and other composition, make to use livestock and poultry that this technology cultivates compared with prior art, mainly there is following some outstanding beneficial effect.
1. Chinese herbal medicine is with trace element in conjunction with having disease-resistant and somatotrophic effect, and the method for the invention completely can substitute antibiotics, reduces micro-consumption simultaneously.
2. reduce micro-consumption, economize on resources, reduce discharge, protection of the environment.
3. antibiotic-free, noresidue, guarantees food security and human health.
4. purify water, the healthy hidden danger that blocking-up is brought to pig by water source.
Embodiment
A kind of fermented forage that keeps healthy described in the embodiment of the present invention coordinates the ecological cultivation method of probiotics fermention bed, as an example of specific experiment case example, embodiment is described below, should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1: the kind of probiotics and fermentation process thereof
This fermentation bed is used bacterial classification to comprise plant lactobacillus, enterococcus faecalis dhf-5, Geotrichum, S. cervisiae, actinomycetes dha-8 and bacillus subtilis dhk-7, plant lactobacillus and enterococcus faecalis dhf-5 are carried out to anaerobic fermentation, and other bacterial classification carries out aerobic fermentation.
Anaerobic fermentation: intact plant lactobacillus and enterococcus faecalis dhf-5 are inoculated in liquid nutrient medium to (1000mL conical flask loading amount is generally 500mL) under anaerobic environment, i.e. 37 ℃ of constant temperature culture 24h under oxygen free condition, in the time of zymotic fluid pH < 4.5, its culture transferring is carried out to one-level expansion cultivation to the fermentation tank of 50L, then 37 ℃ of constant temperature culture 24h under anaerobism (being anaerobic) condition, in the time of zymotic fluid pH < 4.5 in fermentation tank, again its culture transferring is carried out to secondary expansion cultivation to the fermentation tank of 1000L, again under oxygen free condition, 37 ℃ of constant temperature culture 30h, in the time of zymotic fluid pH < 4.5 in fermentation tank, liquid fermentation finishes, obtain the bacterium liquid of anaerobic fermentation.
Aerobic fermentation: by the Geotrichum of preserving, S. cervisiae, actinomycetes dha-8 and bacillus subtilis dhk-7 are inoculated into (1000mL conical flask loading amount is 500mL) in liquid nutrient medium, under aerobic environment, 30 ℃ of constant temperature culture 24h, in the time of zymotic fluid pH < 5.5, its culture transferring is carried out to one-level expansion cultivation to the fermentation tank of 50L, then under aerobic conditions, 30 ℃ of constant temperature culture 24h, in the time of zymotic fluid pH < 5.5 in fermentation tank, its culture transferring is carried out to secondary expansion cultivation to the fermentation tank of 1000L, under aerobic conditions, 30 ℃ of constant temperature culture 30h, when in fermentation tank when zymotic fluid pH < 5.5, liquid fermentation finishes, obtain the bacterium liquid of aerobic fermentation.
Preferably, in above-mentioned anaerobic fermentation and aerobic fermentation, the composition of liquid nutrient medium used comprises: the compositions such as glucose, sulfate of ammoniac, manganese sulphate, magnesium sulfate, potassium dihydrogen phosphate, corn, dregs of beans and deionized water.
The preparation of fermentation bed: first percentage by weight is respectively to 40% sawdust, 40% rice husk, 5% wheat bran, the first compound Chinese herb of 15% mixes, be organic bedding and padding, again the bacterium liquid of the bacterium liquid of anaerobic fermentation and aerobic fermentation is mixed with volume ratio at 1: 1, inoculum concentration by 10% is seeded to mixed bacterium liquid in described organic bedding and padding, water content is controlled at and is no more than 60%, then carry out solid state fermentation in the mode of piling up, after 3-5 days in the time that solid material temperature reaches 50 ℃, organic bedding and padding after fermentation are not less than to 60cm with thickness to be layered on and to be not less than 20m
2fermentation bed on, pig is carried out to ecologic breeding, density accounts for 1.0m for average every pig
2～1.5m
2.This first compound Chinese herb is 80 object powder.
The present invention adopts the ecological cultivation method of probiotics fermention bed thoroughly to solve the pollution problem of raising pigs to environment.Owing to containing the activated beneficial microbe of tool (being above-mentioned several bacterial classifications) in the organic bedding and padding that spread in above-mentioned fermentation bed, it can degrade, digest the fecaluria excreta of pig quickly and effectively, do not need every day and clean swinery, rinse pig house, thereby do not have any discarded object to discharge pig farm, also without any the sewage that rinses colony house, can conserve water more than 70%.Really reach the object of the zero-emission of raising pigs.Improve pig house environment simultaneously, because being designed to large window with the pig house of fermentation bed, Full-open in summer Rolling curtain closet in winter insulation, pig house roof arranges electric exhausting skylight, ventilated good, growth that temp. and humidity is all suitable for pig.Pig manure urine decomposes rapidly through beneficial microbe, in pig house, there is no foul smell, do not grow fly, detect through surrounding air, the pernicious gas content such as the interior carbonic acid gas of microecological fermentation bed pig house, hydrogen sulphide, ammonia, all lower than traditional brooder pig house, are significantly less than the national Ministry of Agriculture " animal or fowls farm environment matter dish standard ".In addition, in the fermentation bed of micro-ecology, use the first compound Chinese herb powder, fermentation bed is further improved, to prevent from growing mould, pathogenic bacteria and parasite etc., greatly reduce the incidence of disease of pig, efficiently solve in the time of pig farm and swinery generation respiratory disease, if not medication, growth speed of pigs is slow, survival rate and growth rate are low, the price of deed reduces, the deficiency that interests lose more than gain.
In addition, be noted that in this is provided with fermentation bed to raise pig pig house and can not use chemical disinfection medicine and antibiotics, if used, will kill and suppress microorganism, or suppress its breeding, make the activity decreased of microorganism; This is also conducive to prevent that people from raising pig with materials such as antibiotic.
Embodiment 2: fermented forage and preparation thereof
1. feed composition: the composition of fermented forage: a. religion groove agent of feed for nursing comprises corn 50kg, dregs of beans 35kg and small powder 15kg, and wherein small powder comprises vitamin 0.2kg, micro-0.06kg, mineral matter 2.91kg, amino acid/11 .2kg, other additive 9.13kg and the second compound Chinese herb 1.5kg; B. pig starter feed comprises corn 55kg, dregs of beans 22kg, peanut meal 5kg, wheat bran 10kg and small powder 8kg, and wherein small powder comprises vitamin 0.2kg, micro-0.05kg, mineral matter 2.91kg, amino acid/11 kg, other additive 2.34kg and the second compound Chinese herb 1.5kg; C. middle hog grower feed comprises corn 55kg, dregs of beans 12kg, cotton dregs 4kg, wheat bran 15kg and small powder 14kg; Wherein small powder comprises vitamin 0.2kg, micro-0.04kg, mineral matter 2.44kg, amino acid 0.8kg, other additive 9.02kg and the second compound Chinese herb 1.5kg.
Wherein, micro-composition and the content thereof in described religion groove agent of feed for nursing is respectively: 0.068 gram of 6.7 grams of alkali copper, 33.4 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 11.7 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite; Micro-composition and content thereof in described pig starter feed are respectively: 0.068 gram of 5.17 grams of alkali copper, 26.7 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 9.3 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite; Micro-composition and content thereof in described middle hog grower feed are respectively: 3.88 grams of alkali copper, 20 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 7 grams, zinc oxide, 0.046 gram of potassium iodide, 0.068 gram of sodium selenite.
Wherein, in described the second compound Chinese herb in religion groove agent of feed for nursing, the amount of various compositions is bark of eucommia 0.1-0.75kg, dandelion 0.01-0.2kg, cordate houttuynia 0.01-0.3kg, oriental wormwood 0.01-0.3kg, bark of ash 0.01-0.3kg, folium isatidis 0.01-0.3kg, barrenwort 0.01-0.3kg, sweet wormwood 0.1-0.6kg, Herba Andrographitis 0.01-0.3kg, black nightshade 0.1-0.5kg, wolf's milk 0.01-0.3kg, hawthorn 0.1-0.5kg, Fructus Hordei Germinatus 0.1-0.5kg, Divine Comedy 0.1-0.5kg; In the second compound Chinese herb described in described pig starter feed, the amount of various compositions is bark of eucommia 0.1-0.45kg, dandelion 0.01-0.2kg, cordate houttuynia 0.01-0.3kg, oriental wormwood 0.01-0.3kg, bark of ash 0.01-0.3kg, folium isatidis 0.01-0.3kg, barrenwort 0.01-0.3kg, sweet wormwood 0.1-0.4kg, Herba Andrographitis 0.01-0.3kg, black nightshade 0.1-0.5kg, wolf's milk 0.01-0.3kg, hawthorn 0.1-0.4kg, Fructus Hordei Germinatus 0.1-0.4kg, Divine Comedy 0.1-0.4kg; In the second compound Chinese herb described in described middle hog grower feed, the amount of various compositions is bark of eucommia 0.1-0.6kg, dandelion 0.01-0.2kg, cordate houttuynia 0.01-0.3kg, oriental wormwood 0.01-0.3kg, bark of ash 0.01-0.3kg, folium isatidis 0.01-0.3kg, barrenwort 0.01-0.3kg, sweet wormwood 0.1-0.6kg, Herba Andrographitis 0.01-0.3kg, black nightshade 0.1-0.5kg, wolf's milk 0.01-0.3kg, hawthorn 0.1-0.4kg, Fructus Hordei Germinatus 0.1-0.4kg, Divine Comedy 0.1-0.4kg.
The feed of this three phases is not all containing antibiotic; This second compound Chinese herb is 80 object herbal powders.The micro-consumption of being combined with the second compound Chinese herb in small powder is compared with positive usual amounts, copper can reduce more than 80% that (the copper consumption in prior art religion groove child care stage to middle pig stage feed is 150-200ppm, in the present invention, copper consumption is 22.5-37.5ppm), iron can reduce more than 45% (now the technology religion groove child care stage in the large pig stage ferro element consumption be 90-200ppm, ferro element consumption 60-100ppm in the present invention), the piggy stage (is that in prior art, to teach the zinc element consumption in groove agent of feed for nursing be 2115ppm, in the present invention, zinc element consumption is 87.5ppm) zinc consumption can reduce more than 90%, in large pig stage zinc consumption can reduce more than 50% (pig stage zinc element consumption 80-150ppm greatly in technology now, in the present invention, zinc element consumption is 52.5-70ppm) etc.
2. breeding strain: a. is by the method for anaerobic fermentation in embodiment 1 cultivate plants lactic acid bacteria and enterococcus faecalis dhf-5, obtain with embodiment 1 in the bacterium liquid of identical anaerobic fermentation.B. cultivate S. cervisiae by the method for aerobic fermentation in embodiment 1, obtain the aerobic fermentation bacterium liquid of S. cervisiae.
3. feed fermentation: after pig feed that each stage feeds is pulverized, take by formula, first other feed except small powder is put in 500 type horizontal mixers, boiling while stirring carries out high-temperature sterilization slaking to 80 ℃ of temperature, keep 20 minutes; Be transported to rotary agitator with elevator again, cooling; After temperature is reduced to below 40 ℃, in other feed, evenly add small powder, evenly mix about 5 minutes, again the aerobic fermentation bacterium liquid of the bacterium liquid of anaerobic fermentation and S. cervisiae is mixed with volume ratio 1: 1, and to the molasses that add 5% in this mixed bacterium liquid, then be seeded in feed and mix by 10% inoculum concentration, water content is controlled at 45%, pile up anaerobic fermentation 3-5 days, in the time of pH < 4.5, just can be for the livestock and poultry such as pig of feeding while having wine flavour.
This ecologic breeding whole process above-mentioned fermented forage of feeding, fermented forage is that microorganism through the present patent application people screening is by the metabolic activity of himself, antinutritional factor in the feed of vegetalitas, animality and mineral material decomposed or transform, producing more easily by the nutrient of feed intake, digestion, absorption.
Effect of herbal mixture is not only relevant with the quantity of its contained trace element, and also the content micro-with some is than relevant, about the existing many research of these forefathers.And utilize microbial conversion Chinese herbal medicine to improve drug effect, it is a kind of well thinking, the object of Chinese herbal medicine fermentation strengthens curative effect, the original performance of change medicine, the expansion medicine variety of Chinese medicine on the one hand, can also make full use of on the other hand the health-care components such as enzyme, acid, polysaccharide that Institute of Micro-biology produces helps medicine, food digestion to absorb, further promote that animal gets well, and increases productivity.
Embodiment 3
Test objective: this test, by adopting the trace elements such as low copper, iron, zinc to add Chinese herbal medicine, is not used antibiotic and high-copper, high zinc, high antibiotic to be contrast experiment, and pig productivity index of correlation is measured, and checks its impact on pig productivity.Test site and time: select test pig farm: free pig farm, test period is from September 26,9 days to 2011 May in 2011, and experimental period 141 days, divides two stages.
Experimental animal and feed: select 28 ages in days wean of birth 80 same periods, the piglet that body weight (actual measurement) be 8.315kg left and right to test, by feed lot Routine Management, routine immunization, presses all pigs that nest is other, sex random packet.Feed is by production reality or recommended formula configuration, and it is identical that same stage is respectively organized basal feed.
Experimental scheme and feeding and management: test is divided into two stages, the newborn piglet stage and in the large pig stage.Test is continuity raising.Ppm in following table 1～4 is constituent content; Below in four tables, process 1 group of test and adopt concrete floor to raise, before test, with hundred poisonings, column home is carried out to thorough disinfection, experimental period, keeps pig free choice feeding (aniseed part material by fermentation), freely drink water (through purifying), in house, ground keeps dry.According to variation of ambient temperature, keep heat insulation work.Immune programme for children immunity routinely, normally does assanation well, timely conventional therapy when pig morbidity.Process 2 groups of tests and adopt fermentation beds of the present invention to raise, experimental period pig free choice feeding, freely drink water.Immune programme for children immunity routinely.
Breast son's stage: be divided at random 2 processing, 4 repetitions of each processing, 10 pigs of each repetition, divide and support in 8 hurdles, carry out feeding experiment, and the young stage of breast comprises latter 2 weeks of wean and piglet stage, in table 1 and table 2.In the large pig stage (pig stage and large pig stage): continue newborn piglet step-by-step test grouping, test is divided into 2 processing, 4 repetitions of each processing, 10 pigs of each repetition, divide and support in 8 hurdles, carry out feeding experiment, in table 3 and table 4.
Table 1
Table 2
Table 3
| Process | The middle pig stage |
| Process 1 | 150ppm?Cu(CuSO 4)+120ppm?Zn(ZnSO 4)+120ppm?Fe(FeSO 4) |
| Process 2 | 22.5ppm alkali copper+52.5ppm Zn (ZnO)+60ppm FeSO 4The+the second compound Chinese herb powder |
Table 4
| Process | The large pig stage |
| Process 1 | 30ppm?Cu(CuSO 4)+80ppm?Zn(ZnSO 4)+90ppm?Fe(FeSO 4) |
| Process 2 | 22.5ppm alkali copper+52.5ppm Zn (ZnO)+60ppm FeSO 4The+the second compound Chinese herb powder |
The result of the test that following table 5～7 is above-mentioned test.
Table 5: newborn weaned piglets index (8-June 11 May) 35 days
| Project | Process 1 | Process 2 |
| Average starting weight (kg) | 7.91 | 8.58 |
| Average end heavy (kg) | 22.93 | 24.4 |
| Average daily gain (g/d) | 429 | 452 |
| Average daily ingestion amount (g/d/ head) | 699 | 696 |
| Diarrhea rate (%) | 4.06 | 2.5 |
| Feedstuff-meat ratio (%) | 1.63 | 1.54 |
Table 6: middle pig productivity index (12-September 23 June) 102 days
| Project | Process 1 | Process 2 |
| Average starting weight (kg) | 22.93 | 24.4 |
| Average end heavy (kg) | 96.98 | 105.49 |
| Average daily gain (g/d) | 726 | 795 |
| Average daily ingestion amount (g/d/ head) | 2233 | 2329 |
| Feedstuff-meat ratio (%) | 3.08 | 2.93 |
Table 7 fur, stool colour and indigestion particle observed result
| Project | Process 1 | Process 2 |
| Skin color | A | B |
| Hair color | A | B |
| Do not digest particle | B | D |
| Stool colour | D | B |
The testing index of table 5～7 and method
1. daily gain (ADG): before formal test starts, finish, weigh and record by hurdle unification respectively, calculate the weightening finish of every hurdle and each group of average weight gain.2. average daily feed intake (ADFI): add up each group of pig feed consumption rate every day, calculate every per day feed intake.Feed intake=[throwing feeding coal (kg)-residue material amount (kg)]/experimental period (number of days).3. diarrhea rate: record a pig diarrhoea number every day, statistics diarrhoea time started, degree and incidence.Diarrhoea degree is by 4 grades of scorings: ight soil half is rare, starchiness, gruel shape have no indigestion particle, water sample shape is shown in indigestion particle, calculates diarrhea rate.4. ight soil: in observation pig stool colour (A grey, B dark color, C grey black, D black) ight soil, (A is visible for indigestion amounts of particles and particle size, greatly, B is visible, obviously, C is visible, less, D is fine and smooth, not obvious) and contrast.5. porcine skin and by a hair situation: observe porcine skin color (A micro-red, B is ruddy without erythema, C, pale) every day, by staple length, gloss (A is general, B is better).
Test result analysis
The breast piglet stage: by table 5 and table 6 data on the whole, respectively organize a newborn piglet daily gain, diarrhea rate and the equal significant difference of feedstuff-meat ratio (P < 0.05).Conclusion (of pressure testing) has 2 points: 1. process 2 groups of (antibiotic-frees, trace element and Chinese herbal medicine fermentation group) with process its impact on newborn weaned piglets of 1 group of (high-copper+high zinc+high antibiotic) paired observation, can be found out by test data, process the average daily gain of 2 groups and process 1 group high by 5.36%, diarrhea rate, feedstuff-meat ratio respectively than processing 1 group low by 38.4%, 5.52%, significant difference, illustrate that processing 2 groups can substitute high-copper, high zinc and high antibiotic completely, and can improve weaned piglets, reduce diarrhea rate and feedstuff-meat ratio.2. the micro-consumption that can find out very intuitively 2 groups of processing from newborn piglets design table and test data table is starkly lower than 1 group of processing, and the indices of growth performance is all better than processing 1 group, element consumption be respectively copper than process 1 group reduce more than 80%, iron than process 1 group reduce more than 45%, zinc is than 1 group of reduction 50-95% of processing, fully demonstrate after Chinese herbal medicine and trace element combination, improve micro-availability, reduce pollution emissions, and saved mineral resources greatly.
Daily observed result: processing 1 group of ight soil is light tone melena, does not obviously digest as seen feed granules; Process 2 groups of piglet ight soil and be dark color just, exquisiteness, does not digest Particle Phase to few, and particle is little.Process 1 group of skin micro-red, glossiness is general; Process 2 groups of porcine skins ruddy (without erythema), glossiness is better.
In the large pig stage: by table 7 data on the whole, each group feed intake, daily gain and the equal difference of feedstuff-meat ratio are obvious, process the daily gain, feed intake of 2 groups respectively than processing 1 group high by 4.3%, 9.5%, and feedstuff-meat ratio is lower by 4.87% than processing 1, significant difference, fully demonstrates the growth promotion advantage of processing 2 groups.
Through daily observed result, processing 1 group is black ight soil, does not all digest as seen feed granules, and skin is micro-red, and gloss is poor; Process 2 groups and be dark ight soil, fine and smooth shaping, does not digest feed granules little, and it is ruddy that hair color glossiness is better organized skin.
Omnidistance feedstuff-meat ratio: processing 1 group of feedstuff-meat ratio average is 2.83, the feedstuff-meat ratio of processing 2 is 2.70, processes 2 whole process and can reduce feedstuff-meat ratio 0.13.Deliver pig for sale by 100 kilograms of accounting, can increase economic efficiency (save feed: 100 kilograms of * (2.83-2.70)=13 kilogram feed, can increase the heavy 13/2.70=4.81 kilogram of pig, per kilogram live pig is by 12 yuan of calculating, can many incomes 4.81*12=57.72 unit) 57.72 yuan.For ten thousand pig farms, economic benefit is considerable.
In sum: the Chinese herbal medicine fermentation group of processing the antibiotic-free of 2 groups, low trace can substitute 1 group of high-copper, high zinc, high antibiotic processing completely, reduce micro-in and there is obvious disease-resistant growth-promoting effect, fully demonstrating it can economize on resources, reduce discharge, alleviating water and soil pollutes, increase economic efficiency and product quality, guarantee the advantages such as food security.
According to above-mentioned experiment case study, we calculate as example take copper wherein, and approximately 1.5 hundred million tons of all kinds of feed total amounts of the annual processing of China, amount to approximately 20,000 tons, metallic copper source.Existing market is mainly used copper sulphate, accounts for more than 95%, and organic copper accounts for less than 5%.If whole operation technique projects can be national saving copper source every year about more than 1.6 ten thousand tons, reduction copper bar is high-volume more than 1.6 ten thousand tons, and the zinc of answering in contrast and the discharge of iron are also in ten thousand tons.Copper per ton source is by 50,000 yuan of calculating, and 800,000,000 yuans of the cost meters that economize on resources, to alleviate that cost that water and soil pollutes runs far deeper than be this numeral, and this is one of copper only just.Therefore the method for the invention has important market using value and prospect.
Embodiment 4
Existing agriculture standard NY/T843-2008 pollution-free food meat and meat products regulation arsenic≤0.05mg/kg, plumbous, ≤ 0.1mg/kg, cadmium≤0.1mg/kg, copper≤10mg/kg, hexachlorcyclohexane≤0.05mg/kg, dichlorodiphenyl trichloroethane≤0.05mg/kg, dichlorvos≤0.02mg/kg, olaquindox, must not detect (< 0.05mg/kg), aureomycin, must not detect (< 0.1mg/kg), chloramphenicol, must not detect (< 0.01mg/kg), clenobuterol hydrochloride, must not detect (< 0.002mg/kg), total plate count≤500000CFU/g, coliform≤1000MPN/100g.
The market pig of raising out by the method for the invention, the result that meat is tested detection through spectrum Buddhist nun is as follows: in pork, arsenic does not detect (< 0.01mg/kg), lead does not detect (< 0.01mg/kg), cadmium does not detect (< 0.01mg/kg), copper does not detect (< 0.1mg*kg), in pork liver, arsenic does not detect (< 0.01mg/kg), plumbous < 0.052mg/kg, cadmium < 0.032mg/kg, copper < 9.53mg/kg, now in pig industry in liver copper residual be generally in meat several times, so that present people dare not eat pork liver at all, but the actual testing result of our product is than residual also low in meat, the consumption of visible reduction Feed Copper element can reach the hazard residue that reduces animal product, people can be relieved edible.Hexachlorcyclohexane does not detect (< 0.01mg/kg), dichlorodiphenyl trichloroethane does not detect (< 0.01mg/kg), dichlorvos does not detect (< 0.01mg/kg), olaquindox does not detect (< 0.04mg/kg), aureomycin does not detect (< 0.005mg/kg), chloramphenicol does not detect (< 0.0001mg/kg), clenobuterol hydrochloride does not detect (< 0.00025mg/kg), total plate count≤9200CFU/g, far below the 500000CFU/g in regulation, coliform≤30MPN/100g, far below the 1000MPN/100 in regulation, meet pollution-free food requirement.So the animal product that this cultural method is produced have been accomplished safety, green, healthy, environmental protection, are the favourable assurances that present people pursue healthy living.
The present invention is not limited to above-mentioned preferred forms, any modification relevant of the present invention or change that anyone does under enlightenment of the present invention, and every have identical with a application or akin technical scheme, within all dropping on protection scope of the present invention.
Claims (10)
1. the fermented forage that keeps healthy coordinates an ecological cultivation method for probiotics fermention bed, it is characterized in that: described ecological cultivation method comprises the following steps:
1. probiotics fermentation: the anaerobic fermentation and the aerobic fermentation that has oxygen animalcule that comprise anaerobe; Described anaerobe comprises plant lactobacillus and enterococcus faecalis dhf-5, described in have oxygen animalcule to comprise Geotrichum, S. cervisiae, actinomycetes dha-8 and bacillus subtilis dhk-7;
Described anaerobic fermentation is that anaerobe is inoculated into and in liquid nutrient medium, carries out anaerobic constant temperature culture, and is expanded and cultivated and secondary expands cultivation and obtains anaerobic fermentation bacterium liquid by one-level; Described aerobic fermentation is that aerobic microbial inoculant is carried out to aerobic constant temperature cultivation in liquid nutrient medium, and is expanded and cultivated and secondary expands cultivation and obtains aerobic fermentation bacterium liquid by one-level;
2. the preparation of probiotics fermention bed: the various raw materials of preparation fermentation bed material are mixed, obtain organic bedding and padding, again anaerobic fermentation bacterium liquid and aerobic fermentation bacterium liquid are seeded in organic bedding and padding, pile up solid state fermentation, after 3-5 days in the time that solid material temperature reaches 50 ℃, by organic bedding and padding uniform spreading, in described fermentation bed, the thickness that spreads is not less than 60cm, then livestock and poultry are carried out to ecologic breeding; Described organic bedding and padding comprise sawdust, rice husk, wheat bran and the first compound Chinese herb, and described the first compound Chinese herb comprises dandelion, cordate houttuynia, oriental wormwood, the bark of ash, folium isatidis, barrenwort, sweet wormwood, Herba Andrographitis, black nightshade, macleaya cordata, wolf's milk, rhizome of cyrtomium and Golden Larch Bark;
3. the composition of fermented forage: a. religion groove agent of feed for nursing comprises corn 50kg, dregs of beans 35kg and small powder 15kg, and wherein small powder comprises vitamin 0.2kg, micro-0.06kg, mineral matter 2.91kg, amino acid/11 .2kg, other additive 9.13kg and the second compound Chinese herb 1.5kg; B. pig starter feed comprises corn 55kg, dregs of beans 22kg, peanut meal 5kg, wheat bran 10kg and small powder 8kg, and wherein small powder comprises vitamin 0.2kg, micro-0.05kg, mineral matter 2.91kg, amino acid/11 kg, other additive 2.34kg and the second compound Chinese herb 1.5kg; C. middle hog grower feed comprises corn 55kg, dregs of beans 12kg, cotton dregs 4kg, wheat bran 15kg and small powder 14kg; Wherein small powder comprises vitamin 0.2kg, micro-0.04kg, mineral matter 2.44kg, amino acid 0.8kg, other additive 9.02kg and the second compound Chinese herb 1.5kg; Described the second compound Chinese herb comprises the bark of eucommia, dandelion, cordate houttuynia, oriental wormwood, the bark of ash, folium isatidis, barrenwort, sweet wormwood, Herba Andrographitis, black nightshade, wolf's milk, hawthorn, Fructus Hordei Germinatus and Divine Comedy;
4. the preparation of fermented forage: a. breeding strain: by step 1. described in the method for anaerobic fermentation make the bacterium liquid of the anaerobic fermentation of plant lactobacillus and enterococcus faecalis dhf-5; By step 1. described in aerobic fermentation method make the aerobic fermentation bacterium liquid of S. cervisiae; B. by step, in the feed described in 3., other feed composition except small powder is put in horizontal mixer, high-temperature sterilization slaking is carried out in boiling to 80 while stirring ℃, then after below being cooled to 40 ℃, add small powder, evenly mix, and by having added the anaerobic fermentation bacterium liquid of 5% molasses and the aerobic fermentation bacterium liquid of S. cervisiae to mix, inoculate to having added in the feed of small powder, pile up anaerobic fermentation 3-5 days, as pH < 4.5, stop fermentation, obtain fermented forage.
2. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: step 1. described in the detailed process of anaerobic fermentation be by the plant lactobacillus of preserving, enterococcus faecalis dhf-5 is inoculated in liquid nutrient medium, 37 ℃ of constant temperature culture 24h under oxygen free condition, in the time of zymotic fluid pH < 4.5, its culture transferring is carried out to one-level expansion cultivation to the fermentation tank of 50L, 37 ℃ of constant temperature culture 24h under oxygen free condition, in the time of zymotic fluid pH < 4.5 in fermentation tank, its culture transferring is carried out to secondary expansion cultivation to the fermentation tank of 1000L, the lower 37 ℃ of constant temperature culture 30h of oxygen free condition, in the time of zymotic fluid pH < 4.5 in fermentation tank, finish fermentation, obtain described anaerobic fermentation bacterium liquid.
3. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: the detailed process of the aerobic fermentation of step described in be 1. by described aerobic microbial inoculant in liquid nutrient medium, the lower 30 ℃ of constant temperature culture 24h of aerobic conditions, in the time of zymotic fluid pH < 5.5, its culture transferring is carried out to one-level expansion cultivation to the fermentation tank of 50L, 30 ℃ of constant temperature culture 24h under aerobic conditions, when in tank when zymotic fluid pH < 5.5, its culture transferring is carried out to secondary expansion cultivation to the fermentation tank of 1000L, 30 ℃ of constant temperature culture 30h under aerobic conditions, when in fermentation tank when zymotic fluid pH < 5.5, finish fermentation, obtain described aerobic fermentation bacterium liquid.
4. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: step 2. described in composition and the parts by weight thereof of organic bedding and padding comprise 40% sawdust, 40% rice husk, 5% wheat bran and the first compound Chinese herb of 15%, in described the first compound Chinese herb, the parts by weight of various compositions are: dandelion 1-2 part, cordate houttuynia 1-2 part, oriental wormwood 1-2 part, bark of ash 1-2 part, folium isatidis 1-2 part, barrenwort 1-2 part, sweet wormwood 1-2 part, Herba Andrographitis 1-2 part, black nightshade 1-2 part, macleaya cordata 2-4 part, wolf's milk 1-2 part, rhizome of cyrtomium 1-3 part and Golden Larch Bark 1-4 part.
5. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: anaerobic fermentation bacterium liquid and the aerobic fermentation bacterium liquid of step described in is 2. to mix with the volume ratio of 1: 1, then is seeded in organic bedding and padding by 10% inoculum concentration.
6. health care fermented forage according to claim 5 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: the water content of postvaccinal described organic bedding and padding is no more than 60%.
7. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: the size of the fermentation bed of step described in is 2. for being not less than 20m
2, described livestock and poultry are pig, average every pig area occupied is 1.0～1.5m
2.
8. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: micro-composition and content thereof in the religion groove agent of feed for nursing of step described in are 3. respectively: 0.068 gram of 6.7 grams of alkali copper, 33.4 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 11.7 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite; Micro-composition and content thereof in described pig starter feed are respectively: 0.068 gram of 5.17 grams of alkali copper, 26.7 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 9.3 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite; Micro-composition and content thereof in described middle hog grower feed are respectively: 0.068 gram of 3.88 grams of alkali copper, 20 grams, ferrous sulfate, 9.5 grams of manganese sulphates, 7 grams, zinc oxide, 0.046 gram of potassium iodide and sodium selenite.
9. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: in second compound Chinese herb of step described in 3., various compositions and parts by weight thereof are bark of eucommia 10-75 part, dandelion 1-20 part, cordate houttuynia 1-30 part, oriental wormwood 1-30 part, bark of ash 1-30 part, folium isatidis 1-30 part, barrenwort 1-30 part, sweet wormwood 10-60 part, Herba Andrographitis 1-30 part, black nightshade 10-50 part, wolf's milk 1-30 part, hawthorn 10-50 part, Fructus Hordei Germinatus 10-50 part, Divine Comedy 10-50 part.
10. health care fermented forage according to claim 1 coordinates the ecological cultivation method of probiotics fermention bed, it is characterized in that: detailed process prepared by the fermented forage of step described in is 4.: a. puts into other feed composition except small powder in horizontal mixer, boiling while stirring to 80 ℃ of temperature are carried out high-temperature sterilization slaking, keep 20 minutes; Be transported to rotary agitator with elevator again, cooling, after temperature is reduced to below 40 ℃, evenly add therein small powder, mix 4～7 minutes with other feed uniform ingredients after sterilizing; B. to the molasses that 3. add 5% with 1: 1 mixed step of volume ratio in the aerobic fermentation bacterium liquid of described anaerobic fermentation bacterium liquid and S. cervisiae, mix to obtain inoculum; C. the inoculum described in step b is seeded to by 10% inoculum concentration in the feed that has mixed small powder in step a, the water content of postvaccinal feed is 40～50%, piles up anaerobic fermentation 3-5 days, while fermenting to pH < 4.5, stop fermentation, obtain fermented forage.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410155490.2A CN103891674A (en) | 2014-04-17 | 2014-04-17 | Ecological cultivation method with health care fermented feed matched with probiotic fermentation bed |
| CN201510108762.8A CN104686847A (en) | 2014-04-17 | 2015-03-12 | Healthcare fermented feed for livestock and poultry breeding |
| CN201510109179.9A CN104761310A (en) | 2014-04-17 | 2015-03-12 | Ecologic organic fertilizer and preparation method thereof |
| CN201510109188.8A CN104620996A (en) | 2014-04-17 | 2015-03-12 | Probiotic fermentation bed for breeding livestock and poultry |
| CN201510108751.XA CN104642259B (en) | 2014-04-17 | 2015-03-12 | A kind of livestock and poultry ecological cultivation method and livestock and poultry product |
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| CN201510109179.9A Pending CN104761310A (en) | 2014-04-17 | 2015-03-12 | Ecologic organic fertilizer and preparation method thereof |
| CN201510108762.8A Pending CN104686847A (en) | 2014-04-17 | 2015-03-12 | Healthcare fermented feed for livestock and poultry breeding |
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- 2015-03-12 CN CN201510109179.9A patent/CN104761310A/en active Pending
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Also Published As
| Publication number | Publication date |
|---|---|
| CN104620996A (en) | 2015-05-20 |
| CN104642259B (en) | 2017-11-03 |
| CN104761310A (en) | 2015-07-08 |
| CN104686847A (en) | 2015-06-10 |
| CN104642259A (en) | 2015-05-27 |
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