CN107996823A - Composite probiotic fermented feed and its preparation method and application - Google Patents
Composite probiotic fermented feed and its preparation method and application Download PDFInfo
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- CN107996823A CN107996823A CN201711432028.2A CN201711432028A CN107996823A CN 107996823 A CN107996823 A CN 107996823A CN 201711432028 A CN201711432028 A CN 201711432028A CN 107996823 A CN107996823 A CN 107996823A
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- China
- Prior art keywords
- feed
- fermented feed
- composite probiotic
- animal
- fermented
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Links
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- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
This application provides a kind of method for preparing composite probiotic fermented feed, it includes treating fermented feed with saccharomycete, bacillus subtilis, lactobacillus plantarum and enterococcus faecium combined ferment, wherein the ferment feed pending includes corn flour, dregs of beans, wheat bran and rapeseed oil.Present invention also provides the purposes that the composite probiotic fermented feed prepared by this method and the composite probiotic fermented feed are used for animal feeding.By preparation method provided by the present invention, the nutritive value of feed can be improved, and improves feed color and luster and animal palatability, promotes feed intake.Composite probiotic fermented feed provided by the present invention can effectively improve breeding performonce fo animals and intestinal health.
Description
Technical field
The invention belongs to livestock feed field, and in particular to composite probiotic fermented feed, further relate to its preparation method and
Purposes.
Background technology
One very long process of the development experience of probiotics, is subject to because its remarkable efficacy and green are without side-effects
The favor of domestic and international researcher.Early in 1908, Russian scientists Metchnikoff just proposed enteron aisle lactic acid bacteria to strong first
The benefit of health, he thinks that these floras can suppress the growth of corrupt flora, so as to extend the service life of body.From this various fermentation
Food progresses into the life of people, for example most common Yoghourt is exactly probiotics fermention and comes, and the inside is contained lactic acid bacteria, protected
Add the largely bacterium living such as Leah lactobacillus.Japan is the country that the one one or two these probiotics push commercialization to, they produce
Commodity have Bifidobacteria food, Bifidobacterium medicine and the health food based on " Bifidobacterium ".At that time due to right
The understanding of Bifidobacterium is more and more, and each major company competitively releases Bifidobacterium product, and probiotic composition is brought to society
Upsurge.But the proposition of probiotics this concept and it be really achieved the development of globalization and mainly appear on American-European countries,
People were striving to find the substitute products of antibiotic at that time.Since the side effect of antibiotic, such as medicament residue, medicine are dirty
The addition for preventing some antibiotic has been give stricted orders by dye, drug resistance of bacterium etc., developed country, while as microecology and animal are cured
Research is goed deep into, and researcher begins attempt to add active bacteria formulation in the feed of animal to reach the growth of improvement animal
The effect of with some diseases are prevented.From 19 century 70s, the U.S. begins one's study and produces active bacteria formulation.European Countries are also successive
Start generally to sell the probiotics for livestock and poultry in the eighties in last century.China on fermentative feedstuff of microbe research origin in
The nineties in last century, due to the influence of a variety of factors, the application development of fermentative feedstuff of microbe does not obtain the attention of people.
Until in recent decades, development that animal husbandry is advanced by leaps and bounds, and the problem of the safety of stockbreeding production is also following.Microorganism sends out
Ferment feed is exactly to rise in this case, because its is safely controllable, is widely used at present in Animal husbandry production.
Enteric microorganism to enteral nutrition material absorb and the health of animal plays an important roll.The micro- life of enteron aisle
Thing mainly includes two classes, it is a kind of be it is beneficial to animal body, be known as beneficial microbe, also referred to as probiotics；One kind is to body
Harmful, it is known as harmful microorganism, the two is a kind of competitive relation, and animal body is by adjusting the balance of the two so that body
Keep fit state.Fermentative feedstuff of microbe can adjust the dynamic equilibrium of intestinal flora, first, because fermentative feedstuff of microbe
In inherently there are substantial amounts of active probiotic, after these active probiotics enter enteron aisle, nutrients is competed with harmful microorganism
Matter and living space, suppress harmful microbe growth and breeding；Two fermentative feedstuff of microbe are typically all under anaerobic
Ferment, a variety of organic acids can be produced during the fermentation, this reduces the pH of feed so that inhibit neutrality,
Aerobic harmful microbe growth and breeding.Study and find according to previous experiments, when the pH of feed is 4.0 or so, can reduce
The quantity of salmonella in feed, so as to reduce the quantity of salmonella in enteron aisle indirectly.In addition, the probiotics in feed is led to
The pH of enteron aisle can be reduced by crossing the organic acid that metabolism produces, and suppress the breeding of other harmful microbes, as lactic acid, acetic acid, propionic acid and
The aliphatic acid such as butyric acid, this inhibitory action is known as chemical barrier, and the endospore that probiotics produces can discharge some polypeptides
Class material come play the role of it is antibacterial, sterilization and defence.Bacillus acidi lactici and bacillus etc. can produce carefully in metabolic process
Rhzomorph, suppresses harmful microbe growth.Experiment shows, probiotics is added in daily ration, can reduce Escherichia coli, salmonella
Deng harmful microbe quantity.Bacillus subtilis fermentation liquor is fed to piglet, lactic acid bacteria and Firmicutes in Jejunum of Piglets
Relative abundance significantly improves；Growing-finishing pig is fed with the liquid feed of lactobacillus-fermented, discovery is detected to its excrement sample,
Lactic acid bacteria level in excrement sample is significantly raised, and the level of Escherichia coli and salmonella significantly reduces.Fermentative feedstuff of microbe
While the ratio of lactic acid bacteria and Escherichia coli in animal intestinal tract is improved, and the cecum flora after adjustable and stable imbalance.
A variety of probiotics are added in feed to ferment, and the macromolecular substances in feed can not only resolved into easy to digest
The small-molecule substance of absorption, and the probiotics in feed can also produce some trophic factors in fermentation process, such as enzyme and Wei Sheng
Element etc..Data shows, when breeding of probiotics can produce various enzymes, as protease, amylase and lipolytic enzyme, hydrolase,
Pectase, dextranase, cellulase, phytase isoreactivity digestive ferment, a variety of B groups vitamins and organic acid, promote animal
Digestion and utilization to feed.In addition, a variety of probiotics are added in feed, it is meant that supplemented with abundant microorganism in feed
Mycoprotein.Therefore, fermentative feedstuff of microbe is not only supplemented with abundant nutriment, but also promote animal to original battalion in feed
The absorption of material is supported, fully meets demand of the animal to feed nutrition.
Feed has strong mellowness smell, has certain attractant effect, can improve the feeding of animal after everfermentation
Amount.And fermented feed can reduce the crude fiber content in feed, improve the palatability of feed.Research finds, growing and fattening
After pig feeding fermentative feedstuff of microbe, its average daily gain is significantly improved；Meat duck is raised with fermentative feedstuff of microbe, is found
Microorganism ferment feed can improve the daily ingestion amount of meat duck；Partial fermentation feed is added in beef concentrate can improve beef cattle to thick
The feed intake and digestibility of feed.
Anti-nutritional factors in feed mainly includes：Protease inhibitor, antagonism vitamin acting factor, external source aggegation
Element, tannin, free gossypol, isothiocyanates, phytate phosphorus and alkaloid etc..These anti-nutritional factors can press down in a different manner
Utilization of the thing to nutriment in feed is braked, causes animal digestion absorption function disorderly, influences the growth of animal.Feed passes through
After fermentation, phytate phosphorus or inorganic phosphate are degraded or separate out in feed, become the free phosphorus easily absorbed by animal.With feeding
Expect longer fermentation times, crude fibre, isothiocyanate content gradually decrease in feed；Microbial fermentation processing hair is carried out to dregs of beans
Existing, the polysaccharide of digestibility and utilization is unable in dregs of beans can be digested absorption again, and the biological transformation ratio of protein significantly improves,
In addition the content of the trace meters such as iron, zinc is added, reduces phytic acid phosphorus content.
Probiotics in fermented feed can be immunized as a kind of non-specific immune-regulating factor, stimulating animal body
Cell, such as stimulates the intrinsic confluent monolayer cells of intestinal mucosa, improves the level of animal body cellular immunity and humoral immunity, is a kind of fine
Immunoactivator, such as.As probiotics can induce beta-alexin to express.Research has shown that, is added in broiler fodder prebiotic
Bacterium, it is possible to increase obvious T cell, bone-marrow-derived lymphocyte vigor and antibody level of serum.A certain amount of probiotics is added in feed,
It can promote the development of broiler chicken immune organ, promote it ripe, so as to improve the immune response ability of animal body.
The content of the invention
On the one hand, this application provides a kind of method for preparing composite probiotic fermented feed, including with saccharomycete
(Saccharomyce), bacillus subtilis (Bacillus subtilis), lactobacillus plantarum
(Lactobacillusplantarum) and enterococcus faecium (Enterococcus faecium) combined ferment treats fermented feed, its
Described in treat that fermented feed includes corn flour, dregs of beans, wheat bran and rapeseed oil.
In one embodiment, this method comprises the following steps：
1) aqueous solution using brown sugar compound concentration as 2-4%；
2) saccharomycete, bacillus subtilis, lactobacillus plantarum and enterococcus faecium kind name are dissolved in the aqueous solution,
Strain is allowed in 36-38 DEG C of activation；
3) plus the aqueous solution dilution step 2) obtained strain mixed solution；
4) the strain mixed solution after step 3) is diluted treats fermented feed with 35mL with described:The ratio of 100g is mixed
Close uniformly, be then sub-packed in fermentation bag, be sealed by fermentation 5-15 days at 35 DEG C.
In a specific embodiment, the dosage of brown sugar described in step 1) for so that the brown sugar described in step 4) with
The quality of the fodder ratio to be fermented is 1:100.
In another embodiment, the use of the saccharomycete, bacillus subtilis, lactobacillus plantarum and enterococcus faecium
Measure to be respectively 250g with the quality of the fodder ratio to be fermented:1t、300g:1t、250g:1t and 200g:1t.
In another embodiment, the use of the saccharomycete, bacillus subtilis, lactobacillus plantarum and enterococcus faecium
Measure as so that the total plate count for treating fermented feed inoculation per g in step 4) is (1-2.5) × 108CFU。
In one embodiment, by weight, the corn flour for treating fermented feed including 60-65%, 15-20%
The rapeseed oil of dregs of beans, the wheat bran of 15-20% and 0.5-1%.
Preferably, the air in the fermentation bag is discharged before fermentation is additionally included in step 4).
Preferably, step 3) is diluted using 37 DEG C of warm water.
On the other hand, present invention also provides the composite probiotic fermented feed prepared by the above method.
Another aspect, present invention also provides the purposes of the composite probiotic fermented feed, including with the compound benefit
Raw bacterium fermented feed feeding animals, for improving the growth performance and/or intestinal health of the animal.Preferably, the animal
For snowy peak black-bone chicken.
By the preparation method of composite probiotic fermented feed provided by the present invention, the nutriture value of feed can be improved
Value, and improve feed color and luster and animal palatability, promote feed intake.Composite probiotic fermented feed provided by the present invention
Breeding performonce fo animals and intestinal health can be effectively improved.
Brief Description Of Drawings
Fig. 1 is with the representative jejunum HE stained slices photo (× 40) of the snowy peak black-bone chicken of different feeds raising.
Fig. 2 is with the representative caecum HE stained slices photo (× 40) of the snowy peak black-bone chicken of different feeds raising.
Embodiment
In order to which technical characteristic, purpose and the beneficial effect of the present invention is more clearly understood, now pass through embodiment pair
Technical scheme is further described.
Method used in following embodiments, is the conventional method in this area without specified otherwise；Agents useful for same and instrument
Device is that can be obtained by common commercial channel without specified otherwise.
Unless otherwise stated, content of material used herein or usage percentage are weight percentage.
1 fermented feed of embodiment prepares and nutrient component determining
Treat that fermented feed amounts to 200kg, weigh 124kg corn flour, 36kg dregs of beans, 36kg wheat brans, 2kg rapeseed oils and 2kg
Compound premix.
Compound premix treats that fermented feed provides for every kilogram：Cu≧200mg、Fe≧1000mg、Zn≧1000mg、Mn≧
1000mg, Se≤2.5mg, I≤15mg, Ca≤10%, Zong Lin≤2.5%, sodium chloride 5%, Dan Jian≤7500mg, vitamin A
≤ 100000IU, vitamin D3≤ 30000IU, vitamin E≤850IU, vitamin B1≤ 30mg, vitamin B2≤ 130mg, dimension
Raw element B6≤ 60mg, vitamin B12≤ 0.3mg, Ye Suan≤22mg, Sheng Wu Su≤3mg, Yan Suan≤600mg, Fan Suan≤45mg, plant
Suan Mei≤4000U.
Brown sugar 2kg is weighed, is dissolved in 37 DEG C of warm water, is configured to the brown sugar aqueous solution that whole solubility is 2-4%.
Weigh saccharomycete, bacillus subtilis, lactobacillus plantarum and enterococcus faecium powder and distinguish 50g, 60g, 50g and 40g, it is molten
In the brown sugar aqueous solution of above-mentioned 2-4%, 2h is placed in 37 DEG C of insulating boxs, makes flora rejuvenation.
The above-mentioned brown sugar aqueous solution containing flora is diluted with suitable 37 DEG C of warm water, and is uniformly mixed, warm water used
Amount causes the brown sugar aqueous solution containing flora after the dilution with when fermented feed mixes, treating the volume ratio of fermented feed and water
For 100g:35mL.
It will treat that fermented feed 200kg is poured on the concrete floor cleaned up, splashed with washbowl and contain bacterium after diluting
The brown sugar aqueous solution of group is mixed when splashing with spades to treating on fermented feed, uniformity coefficient grabbed with hand can hold it is agglomerating without going out
Water, landing voluntarily scatter and are advisable.
Mixing is treated that fermented feed is fitted into 50kg fermentation bags, is sealed with sealing machine, then from exhaust outlet fermentation bag
Interior air is completely exhausted out, and is reused rope and is tightened sack.Fermented feed push-in Constant temperature hatch room, fermentation finally are treated into pack
Temperature is 35 DEG C, and fermentation time is 10 days.The feed fermented distributes strong wine flavour in golden yellow, can promote to move
Thing is searched for food
Conventional nutrient index determining and method：
Crude protein：Using GB/T6432-94, Kjeldahl's method.
Crude fat：Using GB/T6433-94, soxhlet extraction.
Coarse ash：Using GB/T6438-94, Method of burning of high temperature.
Moisture：Using GB/T6435-94.
After feed fermentation, the content of nutritional ingredient is changed.Table 1 is nutrient component determining knot before and after fermented feed
Fruit.The crude protein of feed, crude fat, coarse ash and moisture it is fermented after all increase, wherein crude protein content improves
7.86%, crude ash content improves 16.67%, and moisture improves 168%, difference significantly (p<0.05)；Crude fat
4.21% is improved, the not notable (p of difference>0.05).
Nutrient component determining before and after 1 fermented feed of table
Note：Crude protein, crude fat, coarse ash measure on air bells dry basis.Numerical value is average value ± standard in table
By mistake, with columns, shoulder marking-up parent phase with or the data of nothing between difference not significantly (p>0.05), the female difference person's significant difference of shoulder marking-up
(p<0.05)
Influence of 2 fermented feed of embodiment to snowy peak black-bone chicken growth performance
Experiment is chosen 1 age in days weight and is approached and the snowy peak black-bone chicken 300 of health, is randomly divided into 3 processing, i.e., and 100%
Non- fermented feed group ,+50% non-fermented feed group of 50% fermented feed, 100% fermented feed group, each handle 5 repetitions, often
A 20 chickens of repetition.Pre- to raise 7 days, formal raising 150 days, takes the method for breeding of continuous feeding free choice feeding to be raised.Examination
Test that the result is shown in table 2.
Growth performance index has average daily gain ADG, average daily gain ADFI and feed consumption weightening to compare F/G.Correlation computations
Formula is as follows：
This experiment carries out data statistic analysis using SPSS17.0 software One-wayANOVA programs, and Multiple range test uses
The method of LSD and Duncan, conspicuousness p<0.05.Test data is represented with average value ± standard error.
Mainly carry out statistical analysis to the growth performance of snowy peak black-bone chicken in two stages, including it is average daily gain, flat
Equal daily gain and feed-weight ratio.In 0-4 weeks brood the stage, average daily gain, average daily gain and the material weight of each treatment group
Than there is significant difference (p<0.05).From table 2 respectively specifically from the point of view of, the average daily gain of control group is significantly higher than the 1st,
2 groups of (p<0.05).The average daily gain of control group is then less than experimental group and substantially less than the 1st group of (p<0.05).Expect from the point of view of final
Compare again, control group and the 1st group are without significant difference (p>0.05), but it is significantly higher than the 2nd group of (p<0.05).5-15 weeks incubation stage
When, the average daily gain of control group is substantially less than the 1st group of (p<0.05), with the 2nd group of there was no significant difference (p>0.05).From flat
The average daily gain of control group is substantially less than experimental group (p from the point of view of equal daily gain<0.05).The feed-weight ratio of control group is significantly higher than reality
Test group and substantially less than the 2nd group of (p<0.05).Put down in conclusion fermented feed provided by the present invention can significantly improve animal
Equal daily gain simultaneously reduces feed-weight ratio, energy efficiency.
Influence of 2 fermented feed of table to snowy peak black-bone chicken different phase growth performance
Note：Feed intake in table is on dry matter basis；Control group：100% non-fermented feed, the 1st group：50% does not ferment
The fermented feed of feed+50%；2nd group：100% fermented feed；Numerical value is mean+SD (n=20) in table, same column
In number, shoulder marking-up parent phase with or the data of nothing between difference not significantly (p>0.05), the female difference person's significant difference (p of shoulder marking-up<
0.05) influence of 3 fermented feed of embodiment to snowy peak black-bone chicken intestinal health
In off-test last day, each processing group randomly selects 15 chickens respectively, and neck bloodletting is lethal, and jugular vein is adopted
Blood, after body surface is carried out disinfection with 75% ethanol afterwards, splits abdominal cavity, takes the jejunum and caecum of same position, jejunum and caecum sample
Product are placed in paraformaldehyde and fix, for microsection manufacture.
Selecting haematoxylin Yihong HE staining kits, (haematoxylin Yihong (HE) staining kit, green skies biotechnology are ground
Study carefully institute, Shanghai, China), intestine colibacillosis analysis is carried out, is comprised the following steps that：Section, jejunum and caecal tissue after formaldehyde fixation
Gradient elution using ethanol is carried out, paraffin embedding is immersed after dimethylbenzene is transparent, cuts into slices after precooling；Dyeing, illustrates in strict accordance with kit
Book step will cut into slices and carry out HE dyeing, drip mounting after a small amount of neutral gum, and gravity-flow ventilation is dried, and collect microscopy；Microscopy, tissue are cut
Piece is observed by low power to high power under the microscope, and 3 figures of every section interception, section is selected from every group of 8 animals, with pathology figure
As analysis system (Olympus companies, Japan) analysis height of naps and Crypt depth.Result of the test is respectively displayed on table 3 and table 4
In, and representative slice photo is each provided in fig. 1 and 2.
As shown in Table 3, the height of naps of control group jejunum is substantially less than the 2nd (p<0.05)；From the point of view of Crypt depth, control
Group and the not notable (p of the 1st group of difference>0.05), but the two is all remarkably higher than the 2nd group of (p<0.05).In general its height of naps
With the ratio of Crypt depth, i.e. V/C values, control group and the 1st group are without significant difference (p>0.05), but substantially less than 100% ferments
Feed group (p<0.05).As shown in Table 4, the villus length of the 2nd group of caecum is substantially less than control group (p<0.05)；Control group it is hidden
Nest depth is significantly higher than experimental group (p<0.05).The ratio of height of naps and Crypt depth, control group are substantially less than experimental group (p<
0.05)。
3 jejunal tissue form of table
Note：Control group：100% non-fermented feed, the 1st group：The 50% non-fermented feed of fermented feed+50%；2nd group：
100% fermented feed；Numerical value is mean+SD in table, with columns, shoulder marking-up parent phase with or the data of nothing between it is poor
Different not significantly (p>0.05), the female difference person's significant difference (p of shoulder marking-up<0.05)
4 caecal tissue form of table
Note：Control group：100% non-fermented feed, the 1st group：The 50% non-fermented feed of fermented feed+50%；2nd group：
100% fermented feed；Numerical value is mean+SD in table, with columns, shoulder marking-up parent phase with or the data of nothing between it is poor
Different not significantly (p>0.05), the female difference person's significant difference (p of shoulder marking-up<0.05)
To sum up, fermented feed provided by the present invention can by influencing animal intestinal tract height of naps and Crypt depth, into
And play the effect for improving animal intestinal tract health.
It should be pointed out that for those of ordinary skill in the art, without departing from the inventive concept of the premise,
Various modifications and improvements can be made, these belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention
It should be subject to the appended claims.
Claims (10)
1. a kind of method for preparing composite probiotic fermented feed, including with saccharomycete, bacillus subtilis, lactobacillus plantarum and
Enterococcus faecium combined ferment treats fermented feed, wherein the ferment feed pending includes corn flour, dregs of beans, wheat bran and rapeseed oil.
2. the method as described in claim 1, it comprises the following steps：
1) aqueous solution using brown sugar compound concentration as 2-4%；
2) saccharomycete, bacillus subtilis, lactobacillus plantarum and enterococcus faecium are dissolved in the aqueous solution, allow strain to exist
36-38 DEG C of activation；
3) plus the aqueous solution dilution step 2) obtained strain mixed solution；
4) the strain mixed solution after step 3) is diluted treats fermented feed with 35mL with described:The ratio mixing of 100g is equal
It is even, then it is sub-packed in fermentation bag, is sealed by fermentation 5-15 days at 35 DEG C.
3. method as claimed in claim 2, the dosage of brown sugar is so that red described in step 4) wherein described in step 1)
The sugared and quality of the fodder ratio to be fermented is 1:100.
4. method as claimed in claim 1 or 2, wherein the saccharomycete, bacillus subtilis, lactobacillus plantarum and dung intestines ball
The dosage of bacterium is than being respectively 250g with the quality of the fodder to be fermented:1t、300g:1t、250g:1t and 200g:1t.
5. method as claimed in claim 2, wherein the saccharomycete, bacillus subtilis, lactobacillus plantarum and enterococcus faecium
Dosage for so that in step 4) per g treat fermented feed inoculation total plate count be (1-2.5) × 108CFU。
6. method as claimed in claim 1 or 2, wherein by weight, the corn treated fermented feed and include 60-65%
Powder, the dregs of beans of 15-20%, the rapeseed oil of the wheat bran of 15-20% and 0.5-1%.
7. by the air in the fermentation bag before being additionally included in fermentation in method as claimed in claim 2, wherein step 4)
Discharge.
8. composite probiotic fermented feed prepared by the method any one of claim 1 to 7.
9. the purposes of the composite probiotic fermented feed described in claim 8, including raised with the composite probiotic fermented feed
Animal is fed, for improving the growth performance and/or intestinal health of the animal.
10. purposes as claimed in claim 9, wherein the animal is snowy peak black-bone chicken.
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| CN109938179A (en) * | 2019-04-01 | 2019-06-28 | 华南农业大学 | It is a kind of to improve the gentle fermented feed for solving heat stress of standby milk cow growth performance and its preparation and application |
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| CN110881569A (en) * | 2019-10-17 | 2020-03-17 | 广西中粮生物质能源有限公司 | Preparation method of cassava vinasse fermented feed |
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| CN115176922A (en) * | 2022-06-06 | 2022-10-14 | 北京挑战农业科技有限公司 | Laying hen feed prepared by fermenting carrot by-products through compound microorganisms and production method thereof |
| CN115176922B (en) * | 2022-06-06 | 2023-09-08 | 天津博菲德科技有限公司 | Laying hen feed prepared from carrot byproducts by composite microorganism fermentation and production method of laying hen feed |
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