CN105994955A - Compound deodorant special for livestock and poultry and preparation method and application thereof - Google Patents

Abstract

The invention relates to a compound deodorant special for livestock and poultry and a preparation method and application thereof. The compound deodorant comprises, by mass percentage, 3-4% of urease inhibitor, 5-10% of bacillus subtilis, 10-15% of clostridium butyricum, 1-2% of glucose oxidase, 10-13% of spider enzymes, 8-10% of fructo-oligosaccharide powder, and the balance corn starch. The sum of the contents of the components is 100%. The method for preparing the compound deodorant comprises a step 1 of first weighing glucose oxidase, the urease inhibitor and a certain amount of corn starch to perform premixing, and obtaining a preparation I for standby application; a step 2 of weighing and mixing bacillus subtilis, the fructo-oligosaccharide powder, clostridium butyricum and the spider enzymes, and obtaining a preparation II for standby application; a step 3 of fully mixing the preparation I and the preparation II, finally adding the auxiliary material corn starch to regulated mass, and obtaining the compound deodorant special for livestock and poultry. Various in vivo regulating agents are compounded scientifically and reasonably, the problems that an ordinary deodorant is low in deodorization efficiency, poor in safety and high in addition cost and the like are solved, the deodorization effect is improved, and the production performance of the livestock and poultry is improved.

Description

A kind of special deodorizer of compound poultry, preparation method and its usage

Technical field

The present invention relates to the deodorizer of a kind of animal husbandry technical field of pharmaceuticals, be specifically related to a kind of compound poultry Special deodorizer, preparation method and its usage.

Background technology

In recent years, along with China's aquaculture develops rapidly, intensive large-scale cultivation has become livestock and poultry breeding industry One trend of development.But, the foul smell that livestock and poultry farms in scale is discharged is to the pollution of environment the most not Disconnected aggravation, Production of Livestock and Poultry can be worked the mischief by stench, thus has influence on the performance of livestock and poultry production performance, and The living environment also contributing to surrounding resident is the most healthy.New in formally implement on January 1st, 2015 " Law on Environmental Protection " is referred to as " the tightest environmental law in history ", and contaminating enterprises' dynamics of punishing is greatly reinforced by this method, The enforcement of new environmental law makes farm pollution discharge become one of its accent work, this inundant environmental protection revolution, The speed of shuffling of aquaculture will be accelerated.As can be seen here, the effective of livestock and poultry farm odor pollution controls and pipe Reason, to realizing, scale livestock farming Sustainable Healthy Development is significant.

Poultry house foul gas has 168 kinds, including 9 class organic compound (VFA, alcohols, phenols, acid Class, aldehydes, ketone, amine, thio-alcohol and nitrogen-containing heterocycle compound) and 2 kinds of inorganic matter (NH₃、H₂S)。 Wherein NH₃It is to endanger the most serious gas, and Production of Livestock and Poultry has become the ammonia polluter that the whole world is maximum.

At present, domestic and international feces of livestock and poultry Odor con trol technology mainly has two big classes: a class is external deodorizer, I.e. by the stink sprayed or after absorption directly elimination livestock and poultry feces discharge；Another kind of is internal adjusting control agent, i.e. Deodorizer adds in feedstuff with the form of feed additive, to increase digesting and assimilating thus reducing of forage protein Foul smell discharges, and is the most effective and the most economic control measures.It is broadly divided into physical regulating agent, chemistry Adjusting control agent, plant control agent, the big class of regulator four, the respective mechanism of action is different, and every kind of tune Control agent has certain limitation.

(1) physical regulating agent: main active charcoal, zeolite powder, bentonite etc..They mainly utilize molecule Between Van der Waals'attractive force by odorant adsorb, to reach the purpose of deodorization.But shortcoming is deodorising effect does not fills Point, deodorization rate is relatively low, absorbability passage in time and reduce, when temperature rises, also can put on the contrary Go out adsorbate, so safety is poor.

(2) chemical regulator: mainly have oxidant and biocide two kinds, deodorization speed is fast, but safety is relatively Difference, life-time service may make poultry produce drug resistance, is additionally also easy to environment is produced secondary pollution.

(3) plant control agent: include multiple Chinese medicinal herb deodorizer and vegetable active extract, as Yucca carries Take thing, tea polyphenols, cinnamic aldehyde, garlicin, inulin, Jerusalem artichoke etc..They are mainly as fermentation substrate, can By changing the microorganism in animal and bird intestines and feces and sweat thereof, to change the physicochemical property of fecaluria, And then reduce the generation of stench.But the interpolation cost of certain plants extract is the highest, bearing beyond plant Ability.

(4) regulator: mainly include acidulant, probiotics, prebiotics, enzyme preparation etc..Wherein acid Agent is mainly by reducing digestive tract pH value, it is provided that suitable intestinal environment, thus improves the digestion of protein； On the one hand probiotic bacteria improves the digestibility of protein by setting up dominant microflora, and it can also suppress big simultaneously The Putrefaction of the harmful bacterias such as enterobacteria, makes the harmful substances such as stink reduce；Prebiotics can be by intestinal beneficial bacterium Utilize and promote it to breed, thus improving digesting and assimilating of protein；Enzyme preparation can promote the height of nutrient substance Effect absorbs and utilizes.Although presently commercially available regulator is of all shapes and colors, but its effect is difficult to really, The impact of high temperature, Difficulty during some probiotic bacteria (such as lactic acid bacteria) does not tolerates feed manufacturing simultaneously.

Summary of the invention

The invention provides a kind of special deodorizer of compound poultry, preparation method and its usage, solve general Logical deodorizer odor removal efficient is low, poor stability, interpolation high in cost of production problem, improves deodorizing effect, improves poultry Fowl production performance.

For solving above-mentioned technical problem, the technical solution used in the present invention is:

A kind of special deodorizer of compound poultry: the component and the respective mass fraction that contain are respectively as follows: urase Inhibitor 3-4%, bacillus subtilis 5-10%, Clostridium butyricum 10-15%, glucoseoxidase 1-2%, Arazyme 10-13%, oligofructose powder 8-10%, remaining is corn starch, and above each constituent content sum is 100%.

Further, a preferred embodiment of the present invention: described urease inhibitor is cinnamic aldehyde.

Further, a preferred embodiment of the present invention: in described cinnamic aldehyde raw material, cinnamic aldehyde content is 95%-99%.

Further, a preferred embodiment of the present invention: the described effective viable bacteria content of bacillus subtilis raw material ≥1.0×10¹¹Hundred million CFU/g.

Further, a preferred embodiment of the present invention: described effective viable bacteria content >=10 of Clostridium butyricum raw material Hundred million CFU/g.

Further, a preferred embodiment of the present invention: glucose oxidation in described glucoseoxidase raw material Vigor >=the 10000U/g of enzyme.

Further, a preferred embodiment of the present invention: the vigor of arazyme in described arazyme raw material ≥700000U/kg。

Further, a preferred embodiment of the present invention: oligofructose content in described oligofructose powder raw material For 95%-99%.

The preparation method of the special deodorizer of compound poultry of the present invention, comprises the following steps enforcement:

Step 1, first weighs glucoseoxidase, urease inhibitor and a certain amount of corn starch premix, obtains preparation I is stand-by；

Step 2, weighs bacillus subtilis, oligofructose powder, Clostridium butyricum and arazyme successively, and fully Mixing, obtains Formulation II stand-by；

Step 3, is sufficiently mixed preparation I with Formulation II, is eventually adding adjuvant corn starch to definite quality, Obtain the special deodorizer of compound poultry.

The purposes of the special deodorizer of compound poultry of the present invention, the described special deodorizer of compound poultry adds Being added in feedstuff, addition is 0.01-0.03%.

Beneficial effects of the present invention:

The most cheap plant extract is remarkably improved odor removal efficient, can reduce colony house ammonia concentration, change Kind breeding environment, can reduce again organism metabolism ammonia, improves the poultry utilization to nitrogen: the ammonia in Production of Livestock and Poultry can Being divided into colony house ammonia and organism metabolism ammonia, colony house ammonia is mainly by uric acid, carbamide warp in the feces piled up and bedding and padding Urase decomposes generation, and animal body metabolism ammonia is mainly made up of intestinal ammonia and blood ammonia, and intestinal ammonia is due to animal intestinal In antibacterial uricase, urase decompose the product of the nitrogen-containing compound such as carbamide, uric acid, it and colony house in intestinal Ammonia constitutes exogenous ammonia, endangers animal health；Blood ammonia be mainly derived from amino acid whose various deamination and The ammonia that glutamine hydrolysis is generated by kidney, it addition, uric acid, carbamide decompose product through urase in animal wastes Raw a large amount of ammonias, are absorbed into lung by poultry, enter blood, and therefore the increase of intestinal ammonia and colony house ammonia density is all The rising of ammonia concentration can be caused.Cinnamic aldehyde in this deodorizer formula can significantly reduce the urase in feces and live Property, reduce ammonia nitrogen concentration in slurry of feces and urine, improve total nitrogen conservation rate, delay the decomposition of carbamide to reduce poultry Pouity dwelling place ammonia concentration more than 50%, it can also reduce antibacterial uricase and urease activity in intestinal simultaneously, thus Reduce the organism metabolism ammonia such as intestinal ammonia and blood ammonia, and then improve the poultry body utilization rate to nitrogen, promote poultry machine Body is healthy.

2. probiotic bacteria double action mechanism scalable intestinal microbial population balance, reduces stink and produces, raising production performance: On the one hand two kinds of efficient high-temperature resistant probiotic bacterias in formula can recover and set up dominant microflora (probiotics) in intestinal And microecological balance, owing to profitable strain produces the bateriostatics such as organic acid, hydrogen peroxide and bacteriocin at intestinal Matter, thus the growth of putrefaction bacteria in inhibiting intestinal, reduce urease activity, decrease protein to amine and The conversion of ammonia, makes the content of ammonia and amine in enteral and blood decline, which reduces and excrete with feces The harmful gas such as ammonia, improve air quality in house, decrease environmental pollution.On the other hand, probiotic bacteria By increasing the quantity of digestive tract probiotics, improve secretory volume and the digestive enzyme activity of multiple enzyme in intestinal, Wherein protease, amino oxidase etc. can directly participate in the metabolism of nitrogen substance, improve the utilization rate of nitrogen, subtract The discharge of few ammonia, thus the concentration of ammonia in effectively reducing poultry house, improve feeding environment.Wherein hay bud The bacillus cereuss such as spore bacillus, Clostridium butyricum, can be with spore under poor environment is coerced as the one of probiotic bacteria Form exists, and has the ability of acidproof heat-proof, can produce multiple enzyme and improve the digestibility of feed nutrient, they On the one hand improve the digestibility of protein by setting up dominant microflora, escherichia coli etc. can also be suppressed simultaneously The Putrefaction of harmful bacteria, makes the harmful substances such as stink reduce.Clostridium butyricum secreting amylase, hydrolysis starch And saccharide, and produce a large amount of butanoic acid, on the one hand butanoic acid can maintain intestinal acidity environment to improve the activity of digestive enzyme, On the other hand butanoic acid is the regeneration of gut epithelium histiocyte and the Major Nutrient material repaired, and can repair intestinal in time Road damages, it is ensured that animal intestinal is healthy.Oligofructose powder is uniquely can be by intestinal in feedstuff as a kind of prebiotics The material that road probiotics utilizes and promotes it to breed, can make two kinds of more efficient playing a role of probiotic bacteria.

3. novel green enzyme preparation contributes to improving animal and bird intestines health, raising production performance: glucoseoxidase Have the model of action of its uniqueness as a kind of novel green additive, different from normal enzyme preparation, it is not It is to improve breeding performonce fo animals by the way of improving the digestibility and utilization of nutrient and alleviating antinutritional factor Impact, but on the one hand by with glucose effect in feedstuff and the gluconic acid that produces plays a role, On the other hand, glucoseoxidase is consuming oxygen in glucose mechanism, makes digestive tract be more likely formed Anaerobic environment, promotes the propagation of anaerobism probiotics.So integrating, glucoseoxidase can in intestinal The dual function of acidulant and probiotic bacteria can be played.This novel green enzyme preparation is for animal and bird intestines health Overall improvement has extremely important effect.

4. unique highly active protein enzymatic enters efficient absorption and the utilization of proteinic nitrogen, raising production performance: arazyme It is a kind of high activity metal protease secreted by the symbiotic microorganism (HY-3) in Aranea digestive tract, with mesh Protease conventional on front market is compared, and in addition to having more powerful albuminolysis ability, also has tolerance height The feature of warm, wider pH value, and in animal diets, major part bivalent metal ion is (such as Fe²⁺、Zn²⁺、 Co²⁺、Cu²⁺、Mn²⁺、Ca²⁺And Mg²⁺) activity of enzyme can also be made to improve 2～4 times.It addition, Aranea Enzyme has certain pressing down to many pathogenic bacterium (such as escherichia coli, mycobacteria, staphylococcus aureus etc.) Bacterium activity；Obvious antiinflammatory is had to make all kinds of inflammation (pneumonia, bronchitis, skin infection, sinusitis etc.) With.The effect of this unique highly active protein enzyme is various and efficient, can promote efficient absorption and the utilization of proteinic nitrogen, Thus reduce the release of ammoniacal nitrogen, and then improve production performance.

5. ensure efficient simultaneously, it or a kind of nontoxic, noresidue, the raising of the green safety having no side effect Feed additives.Formula selected by this deodorizer is to plant extract, enzyme preparation, probiotic bacteria, prebiotics Science compounds, and especially plant urease inhibitor cinnamic aldehyde can effectively substitute chemical classes urease inhibitor (phosphorus Acid diamides, acetohrdroxamic acid, benzene a kind of jade etc.) secondary pollution to environment, so relieved poultry can be applied to Cultivation, does not all have any harm to livestock birds health and breeding environment.

6. cost is relatively low and addition is little, is a kind of very economical feed additive.Enrich from china natural resources Canella in extract cinnamic aldehyde can replace expensive plant urease inhibitor Y. flaccida Haw. plant completely Extract, makes feed additive cost be substantially reduced, can be widely popularized use at livestock and poultry farm, not Intended economic benefit can also be received on the premise of increasing excessive cost.

7. preparation is simple, easy to use, it is simple to promote.This deodorizer manufacture method is simple, simultaneously because selecting Enzyme preparation, probiotic bacteria is the most high temperature resistant, do not affected by high temperature during feed manufacturing, so being especially suitable for Promote the use of at feed factory and plant.

Detailed description of the invention

Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described Embodiment be only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention Embodiment, it is every other that those of ordinary skill in the art are obtained under not making creative work premise Embodiment, broadly falls into the scope of protection of the invention.

Raw material used in embodiment: in cinnamic aldehyde raw material, cinnamic aldehyde content is 95%-99%.Hay bud Effective viable bacteria content >=1.0 × 10 of spore bacillus raw material¹¹Hundred million CFU/g.Effective viable bacteria content >=10 of Clostridium butyricum raw material Hundred million CFU/g.Vigor >=the 10000U/g of glucoseoxidase in glucoseoxidase raw material.In arazyme raw material Vigor >=the 700000U/kg of arazyme.In oligofructose powder raw material, oligofructose content is 95%-99%.

Embodiment 1

A kind of special deodorizer of compound poultry, the component contained and respective mass fraction are respectively as follows:

Its preparation method, comprises the following steps:

(1) first weigh glucoseoxidase, cinnamic aldehyde and premix with 300g corn starch, obtaining preparation I Stand-by.

(2) bacillus subtilis, oligofructose powder, Clostridium butyricum, arazyme etc. are weighed the most successively former Expect and be sufficiently mixed, obtaining Formulation II stand-by.

(3) preparation I is sufficiently mixed with Formulation II, be eventually adding residue corn starch to definite quality, i.e. Obtain the special deodorizer of compound poultry.

Embodiment 2

A kind of special deodorizer of compound poultry, the component contained and respective mass fraction are respectively as follows:

Its preparation method, comprises the following steps:

(1) first weigh glucoseoxidase, cinnamic aldehyde and premix with 200g corn starch, obtaining preparation I Stand-by.

(2) bacillus subtilis, oligofructose powder, Clostridium butyricum, arazyme etc. are weighed the most successively former Expect and be sufficiently mixed, obtaining Formulation II stand-by.

(3) preparation I is sufficiently mixed with Formulation II, is eventually adding adjuvant corn starch to definite quality, i.e. Obtain the special deodorizer of compound poultry.

Embodiment 3

A kind of special deodorizer of compound poultry, the component contained and respective mass fraction are respectively as follows:

Its preparation method, comprises the following steps:

(1) first weigh glucoseoxidase, cinnamic aldehyde and premix with 400g corn starch, obtaining preparation I Stand-by.

(2) bacillus subtilis, oligofructose powder, Clostridium butyricum, arazyme etc. are weighed the most successively former Expect and be sufficiently mixed, obtaining Formulation II stand-by.

(3) preparation I is sufficiently mixed with Formulation II, is eventually adding adjuvant corn starch to definite quality, i.e. Obtain the special deodorizer of compound poultry.

Embodiment 4

A kind of special deodorizer of compound poultry, the component contained and respective mass fraction are respectively as follows:

Its preparation method, comprises the following steps:

(1) take the corn starch of 400g, every gram of starch add 80 microgram a-amylase (40000U/g) and The saccharifying enzyme (80000U/g) of 20 micrograms, the response time is 10 hours, and reaction temperature is 60 DEG C, reaction PH is 6.0, obtains micropore corn starch；

(2) take glucoseoxidase, urease inhibitor premixes with step (1) gained micropore corn starch, Obtain preparation I stand-by；

(3) weigh bacillus subtilis, oligofructose powder, Clostridium butyricum and arazyme, and be sufficiently mixed, Obtain Formulation II stand-by；

(4) preparation I is sufficiently mixed with Formulation II, be eventually adding residue corn starch to definite quality, i.e. Obtain the special deodorizer of compound poultry.

The present embodiment uses manganese to compound with urease inhibitor, and suppression urase effect is more preferable, additionally can effectively carry The activity of high enzyme.Additionally by the absorption of micropore corn starch, the bacterium that can effectively heighten microorganism lives, and protects Card has longer shelf life and bacterium lives.

Embodiment 5 cinnamic aldehyde and the deodorizing effect contrast test of Y. flaccida Haw.

Test objective: Y. flaccida Haw. vegetables extractive type deodorant is effective, obtain in Production of Livestock and Poultry extensively should the most abroad With, but its source is limited (being mainly distributed on Southwestern United Stares and Mexico) by region, and the most few in China Seeing, import cost is high so that it is application aborning is very limited.Therefore, cost is sought relatively Low, effect preferably other urease inhibitors have become a kind of inevitable trend.And cinnamon is China Asia The Main Economic seeds of torrid areas, resource is the abundantest, and numerous documents have been studied discovery cinnamon and extracted Thing cinnamic aldehyde can suppress urease activity, and the generation of suppression stool odor, so the purpose of this test is to pass through body Interior test, changes two indices with excrement sample urease activity and fermentation poultry dung ammonia concentration, compares cinnamic aldehyde and silk Blue deodorizing effect.

Test material and instrument: Y. flaccida Haw. plant is " profit can-40 products " of De Jin international corporation of the U.S., Cinnamic aldehyde is the camphor familial plant extract that company of China extracts, Portable Ammonia-air Tester,

Test method:

(1) choose 90 health, 1 aa broiler chicken that body weight is close, male and female is separated, the most at random It is divided into following 3 groups (ensureing often to organize male and female ratio consistent, because sex has certain impact to the speed of growth) as far as possible.

(2) collection of excrement sample and process: test the 21st day and 9:00 in the 42nd day morning collects test chicken 30min Interior discharge is without the fresh excreta polluted.Each experimental group takes 90g fresh excreta in the taper of 500ml In Ping, each conical flask adds 270ml normal saline, the most often organizes and take 10ml solution, 4 DEG C, 4000r/min is centrifuged 5min, takes supernatant and measures wherein urease activity.

Testing inspection index:

(1) chicken manure urease activity: test the 21st day and the 42nd day and use indophenol blue colorimetry detection, urase Activity is in units of the ammonia nitrogen milligram number that every gram of excrement sample of every 24h produces.

(2) fermentation poultry dung ammonia concentration: test the 21st day and the 42nd day and gather each group of fresh chicken manure, and point Not taking equivalent amount to be contained in the plastic envelope bag of sealing, (6h, 12h, 24h) uses ammonia at regular intervals Detector detects wherein ammonia concentration, and this index can intuitively reflect that the various biochemical indicators of fresh chicken manure are to chicken manure The synthesis result of foul smell.

Result of the test:

1. cinnamic aldehyde and the Y. flaccida Haw. comparison (being shown in Table 1) to fresh chicken manure urease activity

Table 1: test the 21st day and the urease activity of the 42nd day fresh chicken manure compares

Note: attached different letter persons represent significant difference (P ＜ 0.05) with column data.

From table 1 result, the cinnamic aldehyde of test group and the Y. flaccida Haw. of positive controls can suppress 21 days and 42 It excrement sample urease activity, significant difference compared with negative control group.

2. the comparison that fermentation poultry dung ammonia concentration is changed by cinnamic aldehyde and Y. flaccida Haw.

Table 2: the ammonia concentration of the 21st day chicken manure fermenting different time sections of test compares

Note: attached different letter persons represent significant difference (P ＜ 0.05) with column data.

From table 2 result, the cinnamic aldehyde of test group and the Y. flaccida Haw. of positive controls can reduce the 21st day chicken The ammonia concentration of manure fermentation different time sections (6h, 12h, 24h), significant difference compared with negative control group.

Table 3: the ammonia concentration of the 42nd day chicken manure fermenting different time sections of test compares

Note: attached different letter persons represent significant difference (P ＜ 0.05) with column data.

From table 3 result, the cinnamic aldehyde of test group and the Y. flaccida Haw. of positive controls can reduce the 42nd day chicken The ammonia concentration of manure fermentation different time sections (6h, 12h, 24h), significant difference compared with negative control group.

Conclusion (of pressure testing): by excrement sample urease activity and fermentation poultry dung ammonia concentration being changed the detection of two indices From the point of view of result, camphor familial plant extract cinnamic aldehyde, as Y. flaccida Haw. plant extract, also can suppress fresh excrement sample Urease activity, thus reduce the ammonia value of fermentation poultry dung, reach the purpose of deodorization, so price is lower Cinnamic aldehyde can replace expensive Y. flaccida Haw. plant extract completely so that it is obtains aborning and is widely popularized, Be a kind of integrate efficiently, the plant type urease inhibitor of safety, economy.

The compound poultry of embodiment 6 special deodorizer formulation screening test

Test objective: according to deodorization index and production performance index, the formula of several composite type deodorization agent is entered The internal screening of row.

Test material and method:

(1) experimental animal and design

Choose 180 health, 1 aa broiler chicken that body weight is close, male and female is separated, divides the most at random For following 6 groups (ensureing often to organize male and female ratio consistent, because sex has certain impact to the speed of growth) as far as possible.

(2) test feedstuff

1-21 age in days feeds basal diet farm 1 material that Shandong feed factory produces, and 22-42 age in days feeds base Plinth daily ration farm 2 material, in order to ensure Feed Energy each deodorizer formula mix homogeneously, so need in advance by Grain fodder crushing.

(3) experiment packet

(4) feeding and management

Feeding experiment is carried out in standardization hen house.Test first 1 week hen house, chicken coop are cleaned thoroughly, Sterilization.Immune programme for children routinely, the 7th age in days inoculates new Zhi Erlian and lives Seedling and newly flow dyad inactivated vaccine, 14 ages in days Inoculation bursa of fabricius vaccine, 21 ages in days inoculation new city vaccines, broiler free choice feeding and drinking-water, every day feeding twice, The feeding time is respectively 8:00 in the morning and 6 weeks 17:30. in afternoon whole experimental periods, carries out (l-3 in two stages Week, 4-6 week).

(5) data statistic analysis

Using SPSS13.0 to carry out one factor analysis of variance and Duncan multiple comparisons, P < 0.05 is significant difference Level, test bit represents with average scholar's standard deviation (Means scholar SD).

Test determination index and method:

1. growth performance:

Weigh on an empty stomach the 3rd weekend and the 6th early morning at weekend, the body weight gains in two stages of record and feed consumption rate, meter Calculate average daily ingestion amount of each phase, average daily gain and feedstuff-meat ratio.

(1) average daily ingestion amount (ADFI)=total feed consumption rate/(test natural law x chicken number)

(2) average daily gain (ADG)=(end weighs an initial weight)/(test natural law x chicken number)

(3) feedstuff-meat ratio (F/G)=average daily ingestion amount/average daily gain

2. blood parameters:

(1) sample collecting and process: use wing Venous Sampling Methods to gather all chicken group's blood (in the 3rd week Collection 6 is often organized at end, often organizes collection 12 the 6th weekend), seal immediately, left at room temperature, treated After serum separates out, 3000rpm is centrifuged 10min, is sub-packed in by supernatant in ampere pipe, sealing.

(2) mensuration of serum urea nitrogen: using urease method to measure, test kit is built up bio-engineering research by Nanjing Thered is provided.

(3) mensuration of blood ammonia: using nonprotein filtrate method to measure, test kit is built up bio-engineering research by Nanjing Thered is provided.

3. intestinal contents biochemical indicator

(1) collection of intestinal samples: dissect chicken take cecal content (in the 3rd weekend often group kill 6 chickens also Sampling, slaughters all chickens the 6th weekend, and often group takes 12 samples), make homogenate with normal saline, centrifugal Take supernatant and carry out following items detection.

(2) mensuration of intestinal ammonia: represent with ammonia nitrogen content, uses indophenol blue colorimetry to measure.

(3) mensuration of uric acid: using phosphotungstic acid colorimetric method for determining, test kit is built up biological engineering by Nanjing and grinds Study carefully and provided.

(4) mensuration of blood urea nitrogen: using urease method to measure, test kit is built up Bioengineering Research Institute by Nanjing There is provided.

Result of the test:

1. each group deodorizer impact on growth of meat chicken performance

The 1.1 respectively group deodorizer impacts on growth of meat chicken early stage (1-3 week) growth performance

Table 1: each group deodorizer impact on growth of meat chicken early stage (1-3 week) growth performance

Remarks: in bracket, percentage ratio is raising or the percentage ratio of reduction compared with index each with matched group 1.

Interpretation of result: from the point of view of table 1 result, compared with matched group 1 group, 2 groups, 5 groups, 6 groups to 1--3 All there is preferably improvement week meat chicken production performance (including average daily ingestion amount, average daily gain and feedstuff-meat ratio) Effect.

The 1.2 respectively group deodorizer impacts on chicken in late growth stage (4-6 week) growth performance

Table 2: each group deodorizer impact on chicken in late growth stage (4-6 week) growth performance

Group	Chicken group's quantity (only)	Average daily ingestion amount (g)	Average daily gain (g)	Feedstuff-meat ratio
					1	23	113.38	51.84	2.19
2	22	(119.71 ↑ 5.58%)	58.39 (↑ 12.64%)	2.05 (↓ 6.39%)
					3	23	114.21	52.39	2.18
4	22	111.04	51.17	2.17
					5	22	(116.23 ↑ 2.51%)	55.35 (↑ 6.77%)	2.10 (↓ 4.11%)
6	23	(121.95 ↑ 7.56%)	61.59 (↑ 18.81%)	1.98 (↓ 9.59%)

Remarks: in bracket, percentage ratio is raising or the percentage ratio of reduction compared with index each with matched group 1.

Interpretation of result: from the point of view of table 2 result, compared with matched group 1 group, 2 groups, 5 groups, 6 groups to 4--6 All there is preferably improvement week meat chicken production performance (including average daily ingestion amount, average daily gain and feedstuff-meat ratio) Effect.

2. each group deodorizer impact on broiler blood parameters

2.1 respectively organize deodorizer to growth of meat chicken early stage (1-3 week) serum urea nitrogen, the impact of Blood Ammonia Concentration

Table 3: each group deodorizer is on growth of meat chicken early stage (1-3 week) serum urea nitrogen, the impact of Blood Ammonia Concentration

Group	Quantity of sampling quantity (only)	Serum urea nitrogen content (mmol/L)	Blood Ammonia Concentration (umol/L)
				1	6	0.53±0.06 a	58.22±2.75 a
2	6	0.48±0.06 ab	57.23±3.45 a
				3	6	0.59±0.05 a	58.68±1.98 a
4	6	0.56±0.04 a	56.36±3.42 a
				5	6	0.43±0.05 b	56.84±4.01 a
6	6	0.40±0.02 b	57.68±4.12 a

Note: attached different letter persons represent significant difference (P ＜ 0.05) with column data.

Interpretation of result: from the point of view of table 3 result, 13 weeks, 2 groups, 5 groups, the serum urea nitrogen content of 6 groups The most on a declining curve compared with matched group 1 group, wherein, 5 groups, the serum urea nitrogen content substantially less than comparison of 6 groups Organize 1 group (P < 0.05)；2 groups, 4 groups, 5 groups, the Blood Ammonia Concentration relatively matched group 1 group of 6 groups all becomes in declining Gesture, but all difference is the most notable.

2.2 respectively organize deodorizer to chicken in late growth stage (4-6 week) serum urea nitrogen, the impact of Blood Ammonia Concentration

Table 4: each group deodorizer is on chicken in late growth stage (4-6 week) serum urea nitrogen, the impact of Blood Ammonia Concentration

Note: attached different letter persons represent significant difference (P ＜ 0.05) with column data.

Interpretation of result: from the point of view of table 4 result, 4--6 week, 2 groups, 5 groups, the serum urea nitrogen content of 6 groups It is substantially less than matched group 1 group (P < 0.05)；2 groups, 3 groups, 4 groups, 5 groups, the Blood Ammonia Concentration of 6 groups more right The most on a declining curve according to organizing 1 group, wherein, 2 groups, 5 groups, the Blood Ammonia Concentration of 6 groups be substantially less than matched group (P<0.05)。

3. each group deodorizer impact on biochemical indicator each in Intestine of Broiler content

3.1 on growth early stage (1-3 week) intestinal ammoniacal nitrogen, uric acid, the impact of urea nitrogen content

Table 5: on growth early stage (1-3 week) intestinal ammoniacal nitrogen, uric acid, the impact of urea nitrogen content

Group	Quantity of sampling quantity (only)	Ammonia nitrogen content (ug/g)	Uric acid content (umol/g)	Urea nitrogen content (mg/g)
					1	6	170.63±20.22 a	0.63±0.17 a	1.18±0.37 a
2	6	154.67±37.32 ab	0.56±0.20 a	1.19±0.62 a
					3	6	115.72±20.46 b	0.60±0.19 a	0.78±0.23 b
4	6	163.52±49.21 a	0.64±0.06 a	1.21±0.52 a
					5	6	178.70±26.15 a	0.77±0.23 a	1.52±0.26 a
6	6	108.16±18.27 b	0.46±0.14 b	0.70±0.15 b

Note: attached different letter persons represent significant difference (P ＜ 0.05) with column data.

Interpretation of result: from the point of view of table 5 result, 13 weeks, 2 groups, 3 groups, 4 groups, the intestinal ammonia content of 6 groups The most on a declining curve compared with matched group 1 group, wherein, 3 groups, the intestinal ammonia content of 6 groups be substantially less than matched group (P<0.05)；2 groups, 3 groups, the uric acid content of 6 groups the most on a declining curve compared with matched group 1 group, wherein, only The uric acid content having 6 groups is substantially less than matched group (P < 0.05)；It addition, 3 groups, the urea nitrogen content of 6 groups It is substantially less than matched group (P < 0.05).

3.2 on Later growth (4-6 week) intestinal ammoniacal nitrogen, uric acid, the impact of urea nitrogen content

Table 6: on Later growth (4-6 week) intestinal ammoniacal nitrogen, uric acid, the impact of urea nitrogen content

Group	Quantity of sampling quantity (only)	Ammonia nitrogen content (ug/g)	Uric acid content (umol/g)	Urea nitrogen content (mg/g)
					1	12	181.20±35.12 a	0.68±0.07 a	3.38±0.54 a
2	12	89.82±25.64 b	0.63±0.08 a	2.79±0.42 b
					3	12	157.09±39.66 ab	0.52±0.04 b	3.47±0.35 a
4	12	163.52±49.21 a	0.64±0.06 a	3.21±0.50 a
					5	12	178.70±26.15 a	0.70±0.08 a	3.17±0.35 a
6	12	92.59±16.25 b	0.54±0.06 b	2.70±0.29 b

Note: attached different letter persons represent significant difference (P ＜ 0.05) with column data.

Interpretation of result: from the point of view of table 6 result, 46 weeks, 2 groups, 3 groups, 4 groups, 5 groups, the intestinal of 6 groups Ammonia content is the most on a declining curve compared with matched group 1 group, wherein, and 2 groups, the intestinal ammonia content substantially less than comparison of 6 groups Group (P < 0.05)；2 groups, 3 groups, 4 groups, the uric acid content of 6 groups the most on a declining curve compared with matched group 1 group, Wherein, 3 groups, the uric acid content of 6 groups be substantially less than matched group (P < 0.05)；It addition, 2 groups, the urine of 6 groups Element nitrogen content is substantially less than matched group (P < 0.05).

Conclusion (of pressure testing):

From the point of view of production performance with each deodorization index comprehensive, 6 groups is the optimization formula filtered out, and various indexs are equal It is better than 3 groups, 4 groups, 5 groups, and under conditions of addition is far below positive controls, each side effect Even it is also advantageous over positive controls 2 groups.It is possible to find out plant extract cinnamic aldehyde, probiotic bacteria prebiotics Compositions, combination of enzyme preparations this three of thing combination between play synergism, it is possible to be obviously improved deodorizing effect, Improve production performance.

Embodiment 7: each deodorizer embodiment deodorizing effect checking test in broiler breeding field

Test objective: check each deodorizer Example formulations to reduce the effect of ammonia concentration in broiler house and right Production performance improves situation.

Test method:

In Shandong, large-scale broiler breeding base is carried out in test, 15 hen houses that alternative condition is close, compares chicken Give up identical with test henhouse structure, breed variety and quantity, feeding and management, 3 drylot feedings of negative control group Feeding the existing feedstuff of chicken house, 3 houses of positive controls add Qingdao company on the basis of existing daily ration Deodorant products, 9 of test group houses add embodiment 1 respectively on the basis of existing daily ration, implement Example 2 and the deodorizer trial target of embodiment 3 formula, specific design is shown in Table 1.

Table 1 broiler breeding field deodorizer EXPERIMENTAL DESIGN

Testing inspection index:

1. production performance index:

When chicken group enters young bird, raise during, record following initial data when delivering for sale: as entered young quantity, raising Period extremely washes in a pan situation, materials quantity, delivers age in days for sale, delivers quantity for sale, delivers body weight, carcass weight etc. for sale, and just Survival rate, deliver the indexs such as (42 days) trunk average weight, feedstuff-meat ratio for sale and be analyzed and compare.

2. blood parameters:

(1) sample collecting and process: use wing Venous Sampling Methods, often organize in time delivering for sale and gather 30 respectively Chicken blood, seals, left at room temperature immediately, and after having serum to separate out, 3000rpm is centrifuged 10min, Supernatant is sub-packed in ampere pipe, sealing.

(2) mensuration of serum urea nitrogen: using urease method to measure, test kit is built up biological engineering by Nanjing and grinds Study carefully and provided.

(3) mensuration of blood ammonia: using nonprotein filtrate method to measure, test kit is built up bio-engineering research by Nanjing Thered is provided.

3. intestinal contents biochemical indicator

(1) collection of intestinal samples: often organize 30 chickens of dissection respectively in time delivering for sale, take cecal content raw Homogenate made by reason saline, and centrifuging and taking supernatant carries out following items detection.

(2) mensuration of intestinal ammonia: represent with ammonia nitrogen content, uses indophenol blue colorimetry to measure.

(3) mensuration of uric acid: using phosphotungstic acid colorimetric method for determining, test kit is built up bio-engineering research by Nanjing Thered is provided.

(4) mensuration of blood urea nitrogen: using urease method to measure, test kit is built up Bioengineering Research Institute by Nanjing and carries Supply.

4. colony house ammonia:

Respectively at 21 ages in days and the 12 noon Portable Ammonia-air Tester before delivering for sale, (there is this instrument in my company Device) carry out colony house ammonia Site Detection, concrete grammar is as follows:

Within 2 hours, simultaneously close off each door and window before testing, respectively every hen house 3 different distributions points, away from The height that ground is 0.9 meter carries out the detection of ammonia value.

Result of the test:

1. the impact on meat chicken production performance

Table 2: each deodorizer embodiment impact on meat chicken production performance

Note: the numerical value in table is meansigma methods ± standard error, indicates same letter and represents that difference is not notable, indicate different letter representation significant difference (P ＜ 0.05)

As can be seen from Table 2, compared with negative control group, each deodorizer formulation Example can improve meat The various production performance indexs of chicken: as significantly improved the survival rate of broiler, it is the heaviest that trunk is delivered in raising for sale, fall Low feedstuff-meat ratio (P ＜ 0.05)；And its each side index is superior to positive controls.

2. the impact on broiler blood parameters

Table 3: each deodorizer embodiment impact on broiler blood parameters (42 age in days)

Note: the numerical value in table is meansigma methods ± standard error, indicates same letter and represents that difference is not notable, indicate different letter representation significant difference (P ＜ 0.05)

As can be seen from Table 3, compared with negative control group, each deodorizer formulation Example can significantly drop The effect of nitrogen metabolism index of correlation in low serum urea nitrogen and Blood Ammonia Concentration (P ＜ 0.05), and its reduction blood Fruit is better than positive controls.

3. the impact on Intestine of Broiler content biochemical indicator

Table 4: each deodorizer embodiment impact on Intestine of Broiler content biochemical indicator (42 age in days)

Note: the numerical value in table is meansigma methods ± standard error, indicates same letter and represents that difference is not notable, indicate different letter representation significant difference (P ＜ 0.05)

As can be seen from Table 4, compared with negative control group, each deodorizer formulation Example can significantly drop Low intestinal ammonia nitrogen concentration also can significantly reduce intestinal uric acid content and urea nitrogen content (P ＜ 0.05), and And it reduces the effect of nitrogen metabolism index of correlation in intestinal and is better than positive controls.

4. the impact on broiler house ammonia concentration

Table 5: each deodorizer embodiment impact on broiler house ammonia concentration

Note: the numerical value in table is meansigma methods ± standard error, indicates same letter and represents that difference is not notable, indicate different letter representation significant difference (P ＜ 0.05)

As can be seen from Table 5, compared with negative control group, each deodorizer formulation Example is to 21 ages in days In hen house, ammonia concentration impact is little, but ammonia concentration (P ＜ 0.05) in 42 age in days hen houses can be significantly reduced, And deodorizing effect is better than positive controls.

Conclusion (of pressure testing):

The each Example formulations of this deodorizer (embodiment 1～embodiment 3) all can effectively reduce Intestine of Broiler content Various nitrogen metabolism indexs of correlation in thing and blood, thus reduce the ammonia concentration in breeding house, and then improve meat The production performance of chicken.

Embodiment 8: each deodorizer embodiment deodorizing effect checking test on pig farm

Test objective: check each deodorizer Example formulations to reduce the effect of ammonia concentration in pig house and to respectively Swine herd production performance improvement situation.

Test method:

(1) test packet: test in Shandong, large-scale Demonstration Base of raising pigs is carried out, alternative condition close 20 Pig house, comparison pig house and to test pig house structure, breed variety identical with quantity, 4 of negative control group Drylot feeding feeds pigs the existing feedstuff in field, and 4 houses of positive controls add Qingdao on the basis of existing daily ration The deodorant products of company, 12 of test group houses add on the basis of existing daily ration respectively embodiment 1, Embodiment 2 and the deodorizer trial target of embodiment 3 formula, specific design is shown in Table 1.Each group correspondence respectively: I Gestation house；II delivery room；III piglet house；IV is bred as house.Timing every morning of each house Measuring ammonia concentration, raise phase 2d in advance, just trying phase 28d, ammonia determines 28d.

Table 1 pig farm deodorizer EXPERIMENTAL DESIGN

(2) pig house structure and management

Test pig is for reaching blue breed system boar, and the whole audience carries out closed management, and in house, temperature is passed to by temperature sensor Computer, is regulated the interior temperature of house by computer regulation rotation speed of the fan and She Nei spray.

Gestation house is half slatted floor, modular, 64 single hurdle (70cm × 230cm)/unit, pig house face Amassing as 10m × 15m, stocking density is 0.43/m2.Feed mode is stockline feeding, quantitative feeding.

Delivery room is full crack obstetric table, modular, 8 single hurdle (180cm × 230cm)/unit, pig house area For 4.5m × 10m, stocking density is 0.18/m2.Hand fed.

Piglet house is full crack obstetric table, modular, 8 circle (120cm × 250cm)/unit, pig house area For 3.5m × 10m, stocking density is 2.06/m2.Free choice feeding, often circle raises 9.

Being bred as house is half crack obstetric table, modular, 12 circle (200cm × 320cm)/unit, pig house area For 7.5m × 16m, stocking density is 0.85/m2.Free choice feeding, often circle raises 8～9.

(3) assay method and data process

Every day experimental period house in press uniform stationing principle selection East, West, South, North, in 5 points, away from Ground 0.3～0.5m is highly located, and timing measures ammonia concentration with portable ammonia instrument in the morning.Data are united Meter is analyzed and is used SPSS10.0 to carry out significance test.

Result of the test:

Respectively organize ammonia concentration (taking weekly a sub-value) (being shown in Table 2)

Table 2 experimental period, respectively organizes ammonia concentration (ppm)

Note: the numerical value in table is meansigma methods ± standard error, indicates same letter and represents that difference is not notable, indicate different letter representation significant difference (P ＜ 0.05)

Start the most aobvious from 1d from table 2 it can be seen that add deodorizer formulation Example 1～embodiment 3 Write and reduce gestation house and be bred as the ammonia concentration (P < 0.05) of house, and start just to significantly reduce product from 7d The ammonia concentration in room, starts just to significantly reduce the ammonia concentration of piglet house from 14d.Positive controls is pregnant It is pregnent house, delivery room, piglet house, ammonia value variation tendency and each test group embodiment variation tendency one of incubation house Cause, simply its except ammonia effect more slightly worse than each test group.

The most each pig house Swine herd production performance improvement situation

Table 3: to gestation house sow and birth piglet each production performance target improvement situation

By table 3 result it can be seen that compared with negative control group, each test group all can improve Farrowing number, Reduce and produce weak young number and stillborn fetus number, improve the number of live birth, and piglet birth counterpoise can be improved.This is mainly Because this deodorizer formula can reduce intestinal ammonia content, reduce the ammonia infringement to intestinal mucosa cells, make intestinal mucosa The renewal of cell reduces, and updates the oxygen consumed and reduces, and sow oxygen content increases, and more oxygen passes through Uterus supply fetus, reduces fetus and forms stillborn fetus or weak tire because of anoxia.

Table 4: production performance target improvement situation each to delivery room ablactational baby pig

By table 4 result it can be seen that compared with negative control group, each test group all can improve nest number pigs weaned, and can improve weaned piglet weight.

Table 5: production performance target improvement situation each to piglet house nursery pig

By table 5 result it can be seen that compared with negative control group, each test group all can improve piglet house child care The survival rate of pig, and can improve 56 age in days nursery pig turn group weight.

Table 6: to being bred as house growing and fattening pigs each production performance target improvement situation

By table 6 result it can be seen that compared with negative control group, each test group all can improve growing-finishing pig Survival rate and 150 ages in days to deliver head for sale the heaviest, and feedstuff-meat ratio can be reduced, high degree improve production Performance.

Conclusion (of pressure testing):

The each Example formulations of this deodorizer (embodiment 1～embodiment 3) can effectively reduce in different cultivars pig house Ammonia concentration, thus improve the production performance of corresponding swinery.

Claims (10)

1. the special deodorizer of compound poultry, it is characterised in that: the component contained and respective mass fraction It is respectively as follows: urease inhibitor 3-4%, bacillus subtilis 5-10%, Clostridium butyricum 10-15%, Fructus Vitis viniferae glycosyloxy Changing enzyme 1-2%, arazyme 10-13%, oligofructose powder 8-10%, remaining is corn starch, above each component Content sum is 100%.

The special deodorizer of the compound poultry of one the most according to claim 1, it is characterised in that: described Urease inhibitor is cinnamic aldehyde.

The special deodorizer of the compound poultry of one the most according to claim 2, it is characterised in that: described meat In cinnamic aldehyde raw material, cinnamic aldehyde content is 95%-99%.

The special deodorizer of the compound poultry of one the most according to claim 1, it is characterised in that: described Effective viable bacteria content >=1.0 × 10 of bacillus subtilis raw material¹¹Hundred million CFU/g.

The special deodorizer of the compound poultry of one the most according to claim 1, it is characterised in that: described The effective viable bacteria content of Clostridium butyricum raw material >=1,000,000,000 CFU/g.

The special deodorizer of the compound poultry of one the most according to claim 1, it is characterised in that: described Glucoseoxidase raw material in the vigor >=10000U/g of glucoseoxidase.

The special deodorizer of the compound poultry of one the most according to claim 1, it is characterised in that: described Arazyme raw material in the vigor >=700000U/kg of arazyme.

The special deodorizer of the compound poultry of one the most according to claim 1, it is characterised in that: described Oligofructose powder raw material in oligofructose content be 95%-99%.

The preparation method of the special deodorizer of a kind of compound poultry the most according to claims 1 to 7, it is special Levy and be, specifically implement according to following steps:

Step 1, first weighs glucoseoxidase, urease inhibitor and a certain amount of corn starch premix, obtains preparation I is stand-by；

Step 2, weighs bacillus subtilis, oligofructose powder, Clostridium butyricum and arazyme successively, and fully Mixing, obtains Formulation II stand-by；

Step 3, is sufficiently mixed preparation I with Formulation II, is eventually adding adjuvant corn starch to definite quality, Obtain the special deodorizer of compound poultry.

10. the purposes of the special deodorizer of compound poultry prepared by claim 9, it is characterised in that: described The special deodorizer of compound poultry add in feedstuff, addition is 0.01-0.03%.