CN109349436A - A kind of preparation method of milk cow liquid complex enzyme for feeding - Google Patents
A kind of preparation method of milk cow liquid complex enzyme for feeding Download PDFInfo
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- CN109349436A CN109349436A CN201811547221.5A CN201811547221A CN109349436A CN 109349436 A CN109349436 A CN 109349436A CN 201811547221 A CN201811547221 A CN 201811547221A CN 109349436 A CN109349436 A CN 109349436A
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- Prior art keywords
- complex enzyme
- milk
- feeding
- milk cow
- liquid complex
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3526—Organic compounds containing nitrogen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
Abstract
The invention discloses a kind of preparation methods of milk cow liquid complex enzyme for feeding, it is related to Animal nutrition and field of feed, fluid present invention complex enzyme cellulase rich in, zytase, amylase, lipase, pectase, dextranase can be improved with lysozyme, arazyme mutual cooperation and improve milk cow to the utilization rate of feed and the rumen digestibility of fibrous matter;Increase the content of albumen in cow's milk;Alleviate environmental stress to the negative effect of milk cow and the effect of mastitis for milk cows can be prevented and treated.
Description
Technical field
The present invention relates to Animal nutritions and field of feed, more particularly to a kind of system of milk cow liquid complex enzyme for feeding
Preparation Method.
Background technique
Mode in Chinese milk cattle cultivating generally to add fine fodder supplement based on roughage, greenfeed, ensilage
Raising, existing milk cattle beneficiated burden material complemental feed generally meet SB/T 10261-1996 in nutritional ingredient and supplement cow concentrate
The requirement of feed but its mainly to supplement the deficiency on the feed nutritions such as roughage, greenfeed, ensilage, but milk cow is to it
The utilization rate of feed is not high, falls flat so as to cause milk cattle beneficiated burden material complemental feed to the promotion of Lactation of Dairy Cow amount, and
It also will affect the butterfat percnetage of milk and the content of lactoprotein, and then influence the quality of dairy products.
Existing milk cattle beneficiated burden material complemental feed is mainly to supplement the feeds such as roughage, greenfeed, ensilage in nutrition
There is above-mentioned disease not for the prevention and treatment ability to common diseases such as the mammitis of milk cow, milk fever, hoof diseases in deficiency
Shape be usually assisted in the treatment of with drug based on, but using drug first is that will increase feeding cost, two understand some using drug
Perhaps residual enters to impact to the quality of milk and the health of people in milk, and third is sick to right during recovery from illness in milk cow
The lactation amount and milk quality of milk cow can also generate certain influence.To cause very huge economic loss to milk cattle cultivating family.
Existing milk cattle beneficiated burden material complemental feed is mostly fed in a manner of powder or particle, and when using powder, enzyme can
To be added in premix, it is uniformly mixed, Direct-fed is entered.If being fed using pellet, pellet in high temperature granulating,
The endogenous enzymes of feed itself and the activity of outer addition enzyme be will lead to by different degrees of loss.
In order to avoid the loss of enzymatic activity when feed granulating, spraying liquid enzyme after granulation, liquid enzymes spraying technology can be used
Spraying process after the feed processing novel as one, can in effective protection feed the heat-sensitive component as enzyme it is effective
Property, reach the optimum efficiency of enzyme feed, improves feed quality.
At present milk cow liquid complex enzyme for feeding substantially with cellulase, zytase, glucolase, pectase these four
Enzyme composition, essentially consists in raising efficiency of feed utilization, improves the digestion power of milk cow, for the mammitis of milk cow, milk fever, hoof disease
Etc. the common diseases problem excessive with milk cow somatic cell score still drug is needed to assist in the treatment of.
Summary of the invention
It, can be with technical problem to be solved by the invention is to provide a kind of milk cow liquid complex enzyme for feeding and preparation method thereof
It is normal to solve mastitis for milk cows, milk fever, hoof disease etc. under the premise of guaranteeing that milk cow has higher efficiency of feed utilization and digestion power
See disease and the excessive problem of milk cow somatic cell score.
A kind of preparation method of milk cow liquid complex enzyme for feeding, it is characterised in that the piglet liquid complex enzyme for feeding by with
Lower step is prepared:
Step 1: broken be crushed to 5mm partial size for material block with crusher for solid fermentation material, a certain amount of water is added and carries out
Stirring obtains wet material for 10-20 minutes；
Step 2: squeezing the juice wet material to obtain filtrate and slag charge with squeezer；
Step 3: filtering: 5% diatomite is added, is filtered with flame filter press and obtains cleaner liquid；
Step 4: anti-corrosion stablize: Lv Qing Ye ︰ Wen Ding 12 ︰ 18 of Ji ︰ preservative=70 ︰ ratio addition glycerol and preservative stir evenly
Both piglet liquid complex enzyme for feeding is obtained.
Further, solid fermentation material cellulase rich in, zytase, the amylase, rouge in step 1
Fat enzyme, pectase, dextranase.
Further, mixing time is 15 minutes in step 1.
Further, stabilizer is that glycerol and xanthan gum are mixed with the ratio of 5:2 in step 4.
Further, preservative is lysozyme, arazyme, glycine, tea polyphenols with the ratio of 25:60:10:15 in step 4
Example cooperates.
Further, the index of milk cow liquid complex enzyme for feeding of the present invention are as follows: zytase 170000-230000U/
Ml, amylase 50000-110000U/ml, lipase 50000-90000U/ml, cellulase 10000-20000U/ml, pectin
Enzyme 20000-50000U/ml, dextranase 85000-130000U/ml.
Present invention fermentation material can contain zytase, starch by its product with Trichoderma, aspergillus niger, bacillus etc.
Enzyme, lipase, cellulase, pectase, one of the strain of dextranase or a variety of finished products obtained through solid state fermentation are mixed
It closes.
It is generally raised by the mode for adding fine fodder supplement based on roughage, greenfeed, ensilage in the raising of milk cow
Support, cellulose rich in, protein in roughage, greenfeed, ensilage, and cow concentrate typically contain corn,
Peanut meal, wheat bran etc. are containing substances such as higher protein, fat, starch, celluloses, and cellulose occupies very big in milk cow forage
Ratio and liquid complex enzyme of the invention contain high activity cellulose and amylase, lipase, dextranase cooperation it is very big
Enhance the digestion power of milk cow, pectin and xylan both substances are all the main component of cell wall, pectase, xylan
Enzyme can decompose pectin and xylan achievees the purpose that destroy cell wall to improve efficiency of feed utilization.
Arazyme used in the present invention is the not metal ion of metalloproteinases and fluid present invention complex enzyme, additional
Glycerol and xanthan gum play the role of enzyme stable, and can effectively prevent arazyme can when milk cow feeds to the decomposition of other enzymes
To activate the activity of arazyme to reach due effect by the metal ion carried in feed.
The antimicrobial spectrum of lysozyme is wider, main -acetylmuramic acid and n-acetylglucosamine by destroying in cell wall
Between β -1,4 glycosidic bond, so that the insoluble glutinous polysaccharide of cell wall is resolved into soluble glycopeptide, cause cell wall rupture content escape
Make bacterolysis out.Lysozyme can also be bound directly with negatively charged virus protein, with DNA, RNA, apoprotein shape
At double salt, make virally inactivated.The enzyme has the effects that antibacterial, anti-inflammatory, antiviral.
Arazyme used in the present invention is high-activity biological secreted by a kind of intracorporal symbiotic microorganism of witch spider
Protease.The protease has very strong antibacterial anti-inflammatory and protein breakdown ability, golden yellow to the main pathogenic bacteria of mastitis for milk cows
The rejection ability of color staphylococcus and Escherichia coli is significantly higher than streptomysin etc., and it also have to pH value, temperature it is stronger
Tolerance.
Lysozyme can destroy the cell wall of bacterium and then reduce the quantity of bacterium, and there is arazyme very strong antibacterial to disappear
It is scorching to have free radical resisting with protein breakdown ability, glycine and tea polyphenols and inhibit the ability of bacterial reproduction, lysozyme can be made
Effect is significantly improved, and arazyme has very the main pathogenic bacteria staphylococcus aureus of mastitis for milk cows and Escherichia coli
Strong rejection ability, cooperating not only increase the holding time of complex enzyme formulation but also have with lysozyme reduces ox
Body cell quantity in milk increases the output of milk and has effects that prevention and treatment mastitis for milk cows.
Compared with prior art, the beneficial effects of the present invention are:
1, the method for liquid complex enzyme of the invention spraying, which is added in feed, avoids the high temperature because of granulation generation to complex enzyme
The influence of vigor and the problem for being directly added into milk cow intake unevenness caused by enzyme preparation.
2, enzyme used in the present invention is arranged in pairs or groups the characteristics of nutritional ingredient in milk cow forage, micro- with cud
Milk cow can be improved to the utilization rate of feed and the rumen digestibility of fibrous matter in bio-cooperation;Increase albumen in cow's milk
Content.Alleviate negative effect of the environmental stress to milk cow.
3, fluid present invention complex enzyme is effectively kept away using arazyme, lysozyme, glycine, tea polyphenols progress preservative treatment
The defect that common preservative such as potassium sorbate can only inhibit bacterial reproduction to be unable to eliminating bacteria on the market is exempted from, largely
On extend the storage time of liquid complex enzyme.
4, in fluid present invention complex enzyme arazyme to the main pathogenic bacteria staphylococcus aureus of mastitis for milk cows and big
Enterobacteria has very strong rejection ability, cooperates with lysozyme and can achieve the effect for preventing and treating mastitis for milk cows.
Specific embodiment
Embodiment one, a kind of preparation method of milk cow liquid complex enzyme for feeding, it is characterised in that the piglet forage liquid
Complex enzyme is prepared from the following steps:
Step 1: broken: the solid containing cellulase, zytase, amylase, lipase, pectase, dextranase being taken to send out
Ferment material 200kg, which is put into crusher, was crushed 5 mesh standard sieves for material block；
Step 2: mixing: will be put into stirring container in step 1 through broken solid fermentation material and add the progress of 200kg water
Stirring obtains wet material for 15 minutes；
Step 3: squeezing: being squeezed the juice wet material with squeezer to obtain 105.7kg filtrate and 194.3kg slag charge；
Step 4: filtering: 5% diatomite is added, is filtered with flame filter press and obtains cleaner liquid 102.1kg；
Step 5: anti-corrosion is stablized: arazyme lysozyme, glycine, tea polyphenols being cooperated with the ratio of 4:3:1:2, anti-corrosion is made
Agent, glycerol and xanthan gum are mixed to prepare stabilizer with the ratio of 5:2, and Lv Qing Ye ︰ Wen is Dinged Ji ︰ preservative with the ratio of 70 ︰, 12 ︰ 18
Example addition glycerol and preservative, which stir evenly, both obtains piglet liquid complex enzyme for feeding.
Step 5: anti-corrosion is stablized: lysozyme, glycine, tea polyphenols are cooperated with the ratio of 5:1:4
Application method: by liquid complex enzyme in a manner of spraying uniform fold in milk cow forage.
Embodiment two, a kind of preparation method of milk cow liquid complex enzyme for feeding, it is characterised in that the piglet forage liquid
Complex enzyme is prepared from the following steps:
Step 1: broken: the solid containing cellulase, zytase, amylase, lipase, pectase, dextranase being taken to send out
Ferment material 200kg, which is put into crusher, was crushed 5 mesh standard sieves for material block；
Step 2: mixing: will be put into stirring container in step 1 through broken solid fermentation material and add the progress of 300kg water
Stirring obtains wet material for 20 minutes；
Step 3: squeezing: being squeezed the juice wet material with squeezer to obtain 309.2kg filtrate and 190.8kg slag charge；
Step 4: filtering: 5% diatomite is added, is filtered with flame filter press and obtains cleaner liquid 307.6kg；
Step 5: anti-corrosion is stablized: arazyme lysozyme, glycine, tea polyphenols being cooperated with the ratio of 4:3:1:2, anti-corrosion is made
Agent, glycerol and xanthan gum are mixed to prepare stabilizer with the ratio of 5:2, and Lv Qing Ye ︰ Wen is Dinged Ji ︰ preservative with the ratio of 70 ︰, 12 ︰ 18
Example addition glycerol and preservative, which stir evenly, both obtains piglet liquid complex enzyme for feeding.
Step 5: anti-corrosion is stablized: lysozyme, glycine, tea polyphenols are cooperated with the ratio of 5:1:4
Application method: by liquid complex enzyme in a manner of spraying uniform fold in milk cow forage.
Embodiment three, a kind of preparation method of milk cow liquid complex enzyme for feeding, it is characterised in that the piglet forage liquid
Complex enzyme is prepared from the following steps:
Step 1: broken: the solid containing cellulase, zytase, amylase, lipase, pectase, dextranase being taken to send out
Ferment material 200kg, which is put into crusher, was crushed 5 mesh standard sieves for material block；
Step 2: mixing: will be put into stirring container in step 1 through broken solid fermentation material and add the progress of 250kg water
Stirring obtains wet material for 17 minutes；
Step 3: squeezing: being squeezed the juice wet material with squeezer to obtain 257.8kg filtrate and 192.2kg slag charge；
Step 4: filtering: 5% diatomite is added, is filtered with flame filter press and obtains cleaner liquid 256.1kg；
Step 5: anti-corrosion is stablized: arazyme lysozyme, glycine, tea polyphenols being cooperated with the ratio of 4:3:1:2, anti-corrosion is made
Agent, glycerol and xanthan gum are mixed to prepare stabilizer with the ratio of 5:2, and Lv Qing Ye ︰ Wen is Dinged Ji ︰ preservative with the ratio of 70 ︰, 12 ︰ 18
Example addition glycerol and preservative, which stir evenly, both obtains piglet liquid complex enzyme for feeding.
Step 5: anti-corrosion is stablized: lysozyme, glycine, tea polyphenols are cooperated with the ratio of 5:1:4
Application method: by liquid complex enzyme in a manner of spraying uniform fold in milk cow forage.
Detection；
The detection of milk cow liquid complex enzyme for feeding obtained by embodiment one to embodiment three enzyme activity result is as follows；
Experiment；
It selects 80 milk production phase milk cows to be tested, is divided into 4 groups of every group of 20 cow heads according to parity, lactation number of days, the output of milk,
Experiment 60 days, in the colony house of the same terms, whole day provides cleaning drinking-water, and daily management provides to carry out according to farm.Daily
It is milked by the worker of profession, ten days feeds fed are that the average value of first group of its feeds utilized output of milk is made before on-test
For the initial output of milk.
First group is control group in four groups of milk cows, and the milk cow forage used is Alfalfa, maize stalks storage feed and milk cow
Fine fodder composition, excess-three group with the milk cow forage for the milk cow liquid complex enzyme for feeding being added in embodiment one, two, three, add respectively
Dosage is feed 100ml per ton.
Experimental result:
It is obvious to the promotion of the output of milk that milk cow forage of the invention is added as can be seen from the above table, using adding in 60 days of experiment
Its output of milk of the milk cow of milk cow forage of the invention is added generally to be not added with the milk production of cow of milk cow forage of the invention than using
It wants high, and is added to milk cow forage of the invention the quantity for reducing milk Methods of Milk Somatic Cell Images can be played and obviously imitate
Fruit, effect highly significant of the present invention to the quantity for reducing milk Methods of Milk Somatic Cell Images compared with being not added with milk cow forage of the invention.
In summary it can be seen that no matter milk cattle fooder adds the milk cow forage of liquid complex enzyme of the invention in the output of milk
Performance with milk cow somatic cell score is all significantly better than the edible milk cattle beneficiated burden material complemental feed for being not added with fluid present invention complex enzyme,
It has been investigated that the excessive number of milk Methods of Milk Somatic Cell Images can be such that lactoprotein in the output of milk and milk of milk cow, butterfat, lactose contains
Amount causes different degrees of decline, and also can increase the disease incidence of mastitis for milk cows, therefore can be from milk Methods of Milk Somatic Cell Images
Quantity and the output of milk are inferred to the content of lactoprotein in the incidence and its milk of mastitis for milk cows, butterfat, lactose, in turn
It may determine that the quality of milk.
Embodiment described above is only presently preferred embodiments of the present invention, is not to make other forms to the present invention
Limitation, the implementation that any those skilled in the art may be changed or be retrofited on the technology contents basis of the disclosure above
Example.Under the premise of without departing from technical solution of the present invention content, the protection scope of technical solution of the present invention should all be fallen into.
Claims (5)
1. a kind of preparation method of milk cow liquid complex enzyme for feeding, it is characterised in that the piglet liquid complex enzyme for feeding is by following
Step is prepared:
Step 1: broken be crushed to 5mm partial size for material block with crusher for solid fermentation material, a certain amount of water is added and carries out
Stirring obtains wet material for 10-20 minutes；
Step 2: squeezing the juice wet material to obtain filtrate and slag charge with squeezer；
Step 3: filtering: 5% diatomite is added, is filtered with flame filter press and obtains cleaner liquid；
Step 4: anti-corrosion stablize: Lv Qing Ye ︰ Wen Ding 12 ︰ 18 of Ji ︰ preservative=70 ︰ ratio addition glycerol and preservative stir evenly
Both piglet liquid complex enzyme for feeding is obtained.
2. a kind of preparation method of milk cow liquid complex enzyme for feeding as described in claim 1, it is characterised in that in step 1
Solid fermentation material cellulase rich in, zytase, amylase, lipase, pectase, dextranase.
3. a kind of preparation method of milk cow liquid complex enzyme for feeding as described in claim 1, it is characterised in that in step 1
Mixing time is 15 minutes.
4. a kind of preparation method of piglet liquid complex enzyme for feeding as described in claim 1, it is characterised in that in step 4
Stabilizer is that glycerol and xanthan gum are mixed with the ratio of 5:2.
5. a kind of preparation method of milk cow liquid complex enzyme for feeding as described in claim 1, it is characterised in that in step 4
Preservative is lysozyme, arazyme, glycine, tea polyphenols are cooperated with the ratio of 25:60:10:15.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811547221.5A CN109349436A (en) | 2018-12-18 | 2018-12-18 | A kind of preparation method of milk cow liquid complex enzyme for feeding |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811547221.5A CN109349436A (en) | 2018-12-18 | 2018-12-18 | A kind of preparation method of milk cow liquid complex enzyme for feeding |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101352203A (en) * | 2008-07-11 | 2009-01-28 | 青岛康地恩生物科技有限公司 | Method for preparing liquid complex enzyme for feeding |
| CN103535510A (en) * | 2013-09-24 | 2014-01-29 | 广州市天朝生物科技有限公司 | Feed for late-stage fattening and lactation promoting of livestock/poultry and ruminants |
| CN105994955A (en) * | 2016-05-19 | 2016-10-12 | 山东信得科技股份有限公司 | Compound deodorant special for livestock and poultry and preparation method and application thereof |
-
2018
- 2018-12-18 CN CN201811547221.5A patent/CN109349436A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101352203A (en) * | 2008-07-11 | 2009-01-28 | 青岛康地恩生物科技有限公司 | Method for preparing liquid complex enzyme for feeding |
| CN103535510A (en) * | 2013-09-24 | 2014-01-29 | 广州市天朝生物科技有限公司 | Feed for late-stage fattening and lactation promoting of livestock/poultry and ruminants |
| CN105994955A (en) * | 2016-05-19 | 2016-10-12 | 山东信得科技股份有限公司 | Compound deodorant special for livestock and poultry and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 孙贵斌等: "蜘蛛酶及其对奶牛生产性能的影响", 《中国奶牛》 * |
| 薛正莲等: "糖化酶稳定性的研究 ", 《食品与发酵工业》 * |
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