CN110074273A - A kind of preparation method of ganoderma lucidum slag fermentation material - Google Patents
A kind of preparation method of ganoderma lucidum slag fermentation material Download PDFInfo
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Abstract
The present invention relates to fermentation arts, in particular to a kind of preparation method of ganoderma lucidum slag fermentation material includes: 3 ~ 10 parts of ganoderma lucidum slag, 5 ~ 13 parts of corn, 2 ~ 7 parts of wheat bran, 4 ~ 14 parts of glucose, 0.5 ~ 1 part of probiotics by weight；Preparation method includes the following steps: that S1. is pre-processed: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 50% ~ 70%, while crush maize is spare；S2. it configures fermentation liquid: probiotics is stirred, add water, fermentation liquid is made；S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding glucose and suitable quantity of water, make moisture content 45% ~ 65%, after being sealed by fermentation 40 ~ 50h, drying obtains the ganoderma lucidum slag fermentation material that moisture content is 8 ~ 12%；Ganoderma lucidum slag fermentation material can be used as agent of feed for nursing sow additive, improve feed intake, milk water and immunity.
Description
Technical field
The present invention relates to fermentation arts, in particular to a kind of preparation method of ganoderma lucidum slag fermentation material.
Background technique
Ganoderma lucidum is also known as Lin Zhongling, fine jade treasure, is the fructification of On Polyporaceae ganoderma lucidum；Ganoderma lucidum has very high natural medicinal
Value and edible value, it is main to contain amino acid, polypeptide, protein, fungal lysozyme and carbohydrate, ergosterol, triterpenes, perfume (or spice)
Beans essence glucoside, volatile oil, stearic acid, benzoic acid, alkaloid, vitamin B2 and C etc.；Spore also contains mannitol, trehalose；Many guarantors
Strong product and pharmaceutical industry are added in health promoting liquid or drug as primary raw material；Large-scale ganoderma lucidum is extracted through water extraction,
It will lead to a large amount of ganoderma lucidum dregs to generate；The ganoderma lucidum dregs for how handling generation become the thorny problem that enterprise faces；At this stage
The abandonment method that abandons or burn mainly directly is buried to the processing means of ganoderma lucidum slag, explores and how to be recycled to waste resource
Method of comprehensive utilization become research direction；It has been investigated that still remaining 17~18% albumen after the extraction of ganoderma lucidum pulp water
Matter, 15 kinds of amino acid, 7.32% the nutriments such as total reducing sugar；This part nutriment can be again through utilizing；In recent years, ganoderma lucidum slag
Comprehensive utilization is mainly reflected in: first is that being used to nursery as biological organic matter；Second is that cultivating specific micro- life as culture substrate
Object；Third is that being used as the raw material of fermented feed；These applications can alleviate ganoderma lucidum Slag treatment bring environmental problem and in reality
Direct productivity effect is generated in the production application of border.
China's feed total output in 2017 is 2.2 hundred million tons, 9809.7 ten thousand tons of pannage, accounting 44%.From Sino-U.S.'s trade in 2018
Easily war outburst, dregs of beans, inevitably by great influence, thus cause feedstuff industry price huge as important feedstuff
Amplitude wave is dynamic；Fermented feed can be used as an important supplement of feedstuff industry at this stage, comes into being and is increasingly becoming Feed Enterprise
Developing direction, fermented feed containing probiotics are that part vegetable raw material in mixed feed is carried out proper fermentation using fermentation technique to add
The feed preparations of work have the characteristics that anti-nutritional factors is low, provide beneficial bacterium for body and a kind of energy-saving and environment-friendly feed；
The complicated multiplicity of probiotics type and huge number, and the probiotics for belonging to enteron aisle in human body or animal body is the most various.With regard to human body
Speech, probiotics is distributed in the stomach function regulating of oral cavity, but far from compared with the type, number of probiotics in enteron aisle, it was found that now
Probiotics in enteron aisle mainly has 6 to belong to as many as 109 kinds, and we are used to the probiotics strain of fermented feed containing probiotics production
It is also incorporated herein mostly.
Fermented feed containing probiotics have the function of that class grows antibiotic, generate growth-promoting effect to body, raw in aquaculture
It is widely used in production.In these years, apparent using harm brought by antibiotic.Therefore, various countries prohibit the use of antibiotic
Enable phase
After sending.Early in 1986, Sweden was put forward for the first time the antibiotic for stopping addition growth-promoting class, there is Denmark, moral successively later
State and Finland propose identical view, and the decree for forbidding feeding antibiotic has been promulgated in European Union within 2006；It can be seen that subtracting
The use of few antibiotic and the use for forbidding antibiotic, undoubtedly promote the process for finding the substitute of antibiotic.Probiotics
Product of the fermented feed as this process can be improved livestock and poultry production performance, enhancing organism disease resistance, without drug with it
The features such as remaining, have no drug resistance and having no toxic side effect becomes one of the novel fodder that substitution feeding antibiotic most has research significance.
Summary of the invention
It is an object of the invention to overcome the above problem, by ganoderma lucidum slag and corn, wheat bran, glucose, probiotics one
It rises, the ganoderma lucidum slag fermentation material of unique effects is made in fermentation in certain circumstances.
The purpose of the present invention is achieved by the following technical programs:
A kind of preparation method of ganoderma lucidum slag fermentation material, the ganoderma lucidum slag fermentation material include: 3 ~ 10 parts of ganoderma lucidum slag by weight, beautiful
5 ~ 13 parts, 2 ~ 7 parts of wheat bran, 4 ~ 14 parts of glucose, 0.5 ~ 1 part of probiotics of rice；The preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 50% ~ 70%, while crush maize is spare；
S2. it configures fermentation liquid: lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould is configured
At probiotics, weight ratio 1:1:1:1:1, number of viable >=1,000,000,000 CFU/g, after mixing evenly, it is 20 ~ 30 DEG C that temperature, which is added,
Fermentation liquid is made in water；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water, make moisture content 45% ~ 65%, and after being sealed by fermentation 40 ~ 50h, drying obtains the spirit that moisture content is 8 ~ 12%
Sesame slag fermentation material.
Ganoderma lucidum slag fermentation material includes a variety of probiotics and relevant metabolite, and contains small point of a large amount of tunning
Sub- polypeptide and amino acid abundant, mineral matter element；The ganoderma lucidum slag fermentation material has stable fragrance, and palatability is good, can add
It is added in agent of feed for nursing sow or pig starter feed to increase milking sow feed intake, it is insufficient to be effectively improved milking sow postpartum milk
Problem；Piglet feed intake is improved, pigling immunity is improved, reduces grice diarrhoea rate.
Preferably, the raw material includes: 5 ~ 8 parts of ganoderma lucidum slag by weight, and 8 ~ 11 parts of corn, 3 ~ 6 parts of wheat bran, Portugal
7 ~ 10 parts of grape sugar, 0.7 ~ 0.9 part of probiotics.
Preferably, the ganoderma lucidum slag is ganoderma lucidum gained after water extraction extracts polysaccharide, and the extraction process is the prior art.
Lactic acid bacteria is that a kind of probiotics applied to fermented feed production, function are using carbohydrate as instead earliest
Substrate is answered to ferment, to generate lactic acid；Saccharomycete is fungi one kind, it is that one kind contains multiple protein in its thallus
The eukaryotic microorganisms of the nutriments such as matter, vitamin, fat, carbohydrate, research find that excellent saccharomycete probiotics can
The output of milk for improving milk cow, makes it generate positive effect to cow producing milk performance；Bacillus subtilis, lichens bacillus subtilis
Pseudomonas is in one kind of bacterium, research shows that it is significant that the case where diarrhea occurs in piglet after adding bacillus subtilis product in diet
It reduces, and the content of blood plasma immunoglobulin M (Ig M) also dramatically increases, in aquaculture, by lichens withered grass gemma
Bacillus is added after feed or water body, and the body immunity of litopenaeus vannamei enhances；Aspergillus is able to suppress harmful toxins, promotes
The growth and development of animal, feed intake improve efficiency of feed utilization, studies have shown that Aspergillus niger strain and enteropathogenic E. Coli carry out
It co-cultures, can obviously inhibit Escherichia coli Growth, and nutritional additive is fabricated to black-koji mould, the growth of animal is sent out
It educates, feed intake and efficiency of feed utilization are all improved.Probiotics has rush to milking sow feed intake, milk, intestinal colony health
Into effect.
Preferably, in the step S3 be sealed by fermentation environment be humidity 50% ~ 70%, 40 ~ 60 DEG C of temperature.
Preferably, the ganoderma lucidum slag fermentation material is used as agent of feed for nursing sow additive.
Preferably, it is 1 ~ 7% that the ganoderma lucidum slag fermentation material, which is added to the weight percent of agent of feed for nursing sow,.
Compared with prior art, the present invention has following technical effect that
It is added in milking sow and/or pig starter feed after the fermentation of ganoderma lucidum slag, milking sow feed intake can be effectively improved, improves
Sow postpartum milk deficiency problem；Improve pigling immunity, reduces grice diarrhoea rate；A variety of probiotics and metabolite are included,
It effectively supplements sow beneficial bacteria of intestinal tract and significantly reduces harmful bacteria quantity, improve sow health；Grice diarrhoea rate is reduced, is promoted and is raised
Expect digestibility, hence it is evident that reduce pig house excrement stink, promote pig house comfort level；Ganoderma lucidum slag fermentation material has good physical attribute, stream
Dynamic property is good, nonhygroscopic and do not agglomerate.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, combined with specific embodiments below, comparative example and reality
Technical solution of the present invention will be described in detail by testing example.Obviously, described embodiment is only present invention a part
Embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making wound
Obtained all other embodiment under the premise of the property made is worked belongs to the range that the present invention is protected.
Except specified otherwise, equipment used in the present embodiment, comparative example and experimental example is routine experiment equipment, used
Reagent be commercially available.
The ganoderma lucidum slag used in the present embodiment can be used the prior art and extract, such as flooding extracts remnants after polysaccharide
Substance, or directly purchased from pharmaceutical factory, about sought containing 17~18% protein, a variety of amino acid, 7.32% total reducing sugar etc.
Support substance.
Embodiment 1
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 7 parts of ganoderma lucidum slag by weight, and 9 parts of corn,
5 parts of wheat bran, 10 parts of glucose, 0.8 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 60%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould according to
Parts by weight content ratio is that 1:1:1:1:1 is configured to probiotics, and number of viable >=1,000,000,000 CFU/g after mixing evenly by probiotics add
Enter the water that temperature is 25 DEG C, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 55%, and after being sealed by fermentation 48h, drying obtains the ganoderma lucidum slag that moisture content is 10% and ferments
Object.
Embodiment 2
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 3 parts of ganoderma lucidum slag by weight, and 5 parts of corn,
2 parts of wheat bran, 4 parts of glucose, 0.5 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 50%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould according to
Parts by weight content ratio is that 1:1:1:1:1 is configured to probiotics, and number of viable >=1,000,000,000 CFU/g after mixing evenly by probiotics add
Enter the water that temperature is 25 DEG C, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 45%, and after being sealed by fermentation 40h, drying obtains the ganoderma lucidum slag that moisture content is 8% and ferments
Object.
Embodiment 3
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 10 parts of ganoderma lucidum slag by weight, corn 13
Part, 7 parts of wheat bran, 14 parts of glucose, 1 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 70%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould according to
Parts by weight content ratio is that 1:1:1:1:1 is configured to probiotics, and number of viable >=1,000,000,000 CFU/g after mixing evenly by probiotics add
Enter the water that temperature is 25 DEG C, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 65%, and after being sealed by fermentation 50h, drying obtains the ganoderma lucidum slag that moisture content is 12% and ferments
Object.
Comparative example 1
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 1 part of ganoderma lucidum slag by weight, and 9 parts of corn,
5 parts of wheat bran, 10 parts of glucose, 0.8 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 60%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould according to
Parts by weight content ratio is that 1:1:1:1:1 is configured to probiotics, and number of viable >=1,000,000,000 CFU/g after mixing evenly by probiotics add
Enter the water that temperature is 25 DEG C, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 55%, and after being sealed by fermentation 48h, drying obtains the ganoderma lucidum slag that moisture content is 10% and ferments
Object.
Compared with Example 1, ganoderma lucidum slag content is lower than content of the invention to this comparative example.
Comparative example 2
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 13 parts of ganoderma lucidum slag by weight, and 9 parts of corn,
5 parts of wheat bran, 10 parts of glucose, 0.8 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 60%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould according to
Parts by weight content ratio is that 1:1:1:1:1 is configured to probiotics, and number of viable >=1,000,000,000 CFU/g after mixing evenly by probiotics add
Enter the water that temperature is 25 DEG C, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 55%, and after being sealed by fermentation 48h, drying obtains the ganoderma lucidum slag that moisture content is 10% and ferments
Object.
Compared with Example 1, ganoderma lucidum slag content is higher than content of the invention to this comparative example.
Comparative example 3
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 7 parts of ganoderma lucidum slag by weight, and 9 parts of corn,
5 parts of wheat bran, 10 parts of glucose, 0.8 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 60%, while crush maize is spare；
S2. fermentation liquid is configured: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis according to parts by weight content
Than being configured to probiotics for 1:1:1:1, number of viable >=1,000,000,000 CFU/g, after mixing evenly by probiotics, it is 25 DEG C that temperature, which is added,
Water, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 55%, and after being sealed by fermentation 48h, drying obtains the ganoderma lucidum slag that moisture content is 10% and ferments
Object.
This comparative example compared with Example 1, lacks black-koji mould in step S2.
Comparative example 4
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 7 parts of ganoderma lucidum slag by weight, and 9 parts of corn,
5 parts of wheat bran, 10 parts of glucose, 0.8 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 60%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, black-koji mould according to parts by weight content ratio be 1:1:1 be configured to it is prebiotic
Bacterium, number of viable >=1,000,000,000 CFU/g after mixing evenly by probiotics are added the water that temperature is 25 DEG C, fermentation liquid are made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 55%, and after being sealed by fermentation 48h, drying obtains the ganoderma lucidum slag that moisture content is 10% and ferments
Object.
This comparative example compared with Example 1, lacks bacillus subtilis and lichens bacillus subtilis in step S2.
Comparative example 5
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 7 parts of ganoderma lucidum slag by weight, and 9 parts of corn,
5 parts of wheat bran, 10 parts of glucose, 0.8 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 60%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould according to
Parts by weight content ratio is that 0.5:0.5:1:1:1 is configured to probiotics, and number of viable >=1,000,000,000 CFU/g stirs evenly probiotics
Afterwards, the water that temperature is 25 DEG C is added, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 55%, and after being sealed by fermentation 48h, drying obtains the ganoderma lucidum slag that moisture content is 10% and ferments
Object.
Compared with Example 1, lactic acid bacteria, yeast bacterial content are lower than content of the present invention to this comparative example in step S2.
Comparative example 6
A kind of preparation method of ganoderma lucidum slag fermentation material, ganoderma lucidum slag fermentation material include: 7 parts of ganoderma lucidum slag by weight, and 9 parts of corn,
5 parts of wheat bran, 10 parts of glucose, 0.8 part of probiotics；Preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 60%, while crush maize is spare；
S2. configure fermentation liquid: by lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould according to
Parts by weight content ratio is that 1:1:0.5:0.5:1 is configured to probiotics, and number of viable >=1,000,000,000 CFU/g stirs evenly probiotics
Afterwards, the water that temperature is 25 DEG C is added, fermentation liquid is made；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water make moisture content up to 55%, and after being sealed by fermentation 48h, drying obtains the ganoderma lucidum slag that moisture content is 10% and ferments
Object.
Compared with Example 1, bacillus subtilis, lichens bacillus subtilis bacterial content are lower than this in step S2 for this comparative example
Invention content.
Experimental example 1
Observe the average daily feed intake of sow nursery phase.
The feeding effect for illustrating 1 ~ 6 feed of the present embodiment 1 ~ 3 and comparative example by specifically testing chooses 400 pregnancies
85 days, the female Landraces of 3~6 parity, according to production performance, the expected date of childbirth is randomly divided into 10 groups, every group of 40 repetitions；Test period
After gestation 85 days to 21 age in days of piglet wean, totally 51 days.It is grouped as follows: the feeding of 100% basal feed of control group, experimental group:
97% basal feed is then respectively adding 3% embodiment 1 ~ 3 and the ganoderma lucidum slag fermentation material of comparative example 1 ~ 6 is fed.Record lactation
Sow records average daily gain from childbirth to the feed intake during weaned piglet.Experimental data with mean ± standard deviation (±
S) it indicates, is examined using t.
The 1 average daily feed intake of sow nursery phase of table
| Group | Basal feed | Ganoderma lucidum slag fermentation material | Average daily feed intake (kg/ head/day) | Group | Basal feed | Ganoderma lucidum slag fermentation material | Average daily feed intake (kg/ head/day) |
| 1 | 100% | 0% | 4.77±0.11 | 6 | 97% | 3% comparative example 2 | 4.84±0.20 |
| 2 | 97% | 3% embodiment 1 | 5.32±0.14 | 7 | 97% | 3% comparative example 3 | 4.92±0.19 |
| 3 | 97% | 3% embodiment 2 | 5.29±0.21 | 8 | 97% | 3% comparative example 4 | 5.21±0.15 |
| 4 | 97% | 3% embodiment 3 | 5.33±0.12 | 9 | 97% | 3% comparative example 5 | 5.01±0.16 |
| 5 | 97% | 3% comparative example 1 | 4.94±0.09 | 10 | 97% | 3% comparative example 6 | 5.25±0.24 |
The experimental results showed that the experimental group of addition ganoderma lucidum slag fermentation material is improved on the influence of milking sow feed intake, it was demonstrated that
Ganoderma lucidum slag fermentation material plays the role of part instead of basal feed in sow diets, while having side to the raising of sow feed intake
It helps, comparative example 1 and 2 can all reduce sow feed intake as can be seen that ganoderma lucidum slag is higher or lower than amount of the invention；Comparative example 3
The missing of black-koji mould and the reduction of comparative example 5 lactic acid bacteria, yeast bacterial content, reduce the increase of the feed intake of sow；Comparison
The missing of example 4 and 6 bacillus subtilis of comparative example and lichens bacillus subtilis and reducing sow feed intake is increased influence compared with
It is small.
Experimental example 2
Observe the wean counterpoise and daily gain of sow nursery phase weanling pig.
The female Landrace for choosing 400 pregnancies 85 days, 3~6 parity, according to production performance, the expected date of childbirth is randomly divided into 10 groups,
Every group of 40 repetitions.Test period after gestation 85 days to 21 age in days of piglet wean, totally 51 days.It is grouped as follows: control group 100%
Basal feed feeding, experimental group: 97% basal feed is then respectively adding the ganoderma lucidum slag fermentation of 3% embodiment 1 ~ 3 and comparative example 1 ~ 6
Object is fed.The individual counterpoise of piglet birth, the individual counterpoise of wean on the 21st are recorded, piglet lactation daily gain is calculated.
The wean counterpoise and daily gain of 2 sow nursery phase weanling pig of table
| Group | Basal feed | Ganoderma lucidum slag fermentation material | The individual counterpoise of birth | Wean individual weight (kg) on the 21st | Piglet average daily gain (kg/d, 1-21) |
| 1 | 100% | 0% | 1.59 | 5.61 | 0.191 |
| 2 | 97% | 3% embodiment 1 | 1.51 | 6.12 | 0.219 |
| 3 | 97% | 3% embodiment 2 | 1.57 | 6.28 | 0.224 |
| 4 | 97% | 3% embodiment 3 | 1.58 | 6.23 | 0.221 |
| 5 | 97% | 3% comparative example 1 | 1.54 | 5.86 | 0.206 |
| 6 | 97% | 3% comparative example 2 | 1.60 | 5.91 | 0.205 |
| 7 | 97% | 3% comparative example 3 | 1.52 | 5.84 | 0.205 |
| 8 | 97% | 3% comparative example 4 | 1.59 | 5.92 | 0.206 |
| 9 | 97% | 3% comparative example 5 | 1.53 | 5.86 | 0.206 |
| 10 | 97% | 3% comparative example 6 | 1.55 | 5.79 | 0.202 |
The experimental results showed that the average daily gain of piglet nursery phase is improved, illustrate that sow milk is sufficient, feeding addition spirit
The feed of sesame slag fermentation material improves piglet weaning weight on the 21st, and helpful to piglet nursery phase daily gain, comparative example 1 and 2 can
To find out, ganoderma lucidum slag, which is higher or lower than amount of the invention, can all be such that the increase of sow milk water reduces；3 ~ 6 probiotics of comparative example
Excalation or reduction, the increase of sow milk water can all reduced.
Experimental example 3
Parturient sow colostrum in experimental example 1 is analyzed, and analyzes milk composition index.
3 milk composition index of table
| Group | Basal feed | Ganoderma lucidum slag fermentation material | Crude fat content (g/100g) | Crude protein content (g/100g) | Non-fat solid content (g/100g) | Lactose content (g/100g) |
| 1 | 100% | 0% | 4.74 | 8.41 | 21.79 | 11.63 |
| 2 | 97% | 3% embodiment 1 | 5.54 | 10.25 | 25.91 | 13.85 |
| 3 | 97% | 3% embodiment 2 | 5.32 | 10.11 | 25.22 | 13.56 |
| 4 | 97% | 3% embodiment 3 | 5.43 | 10.31 | 25.47 | 13.68 |
| 5 | 97% | 3% comparative example 1 | 5.01 | 9.21 | 23.54 | 12.14 |
| 6 | 97% | 3% comparative example 2 | 5.07 | 9.25 | 24.01 | 12.38 |
| 7 | 97% | 3% comparative example 3 | 4.91 | 8.96 | 24.11 | 12.17 |
| 8 | 97% | 3% comparative example 4 | 5.11 | 10.09 | 24.91 | 13.14 |
| 9 | 97% | 3% comparative example 5 | 4.96 | 9.62 | 24.13 | 12.47 |
| 10 | 97% | 3% comparative example 6 | 5.13 | 10.12 | 25.02 | 13.21 |
The experimental results showed that adding Ganoderma fermented product in sow diets improves crude fat in colostrum, crude protein, non-fat solid content
With the content of lactose.Comparative example 1 and 2 is as can be seen that ganoderma lucidum slag can all make in sow colostrum higher or lower than amount of the invention
The increase of milk composition reduces；The missing and reduction pair of comparative example 4 and 6 bacillus subtilis of comparative example and lichens bacillus subtilis
The increased reduction of ingredient influences smaller；5 lactic acid bacteria of missing and comparative example of 3 black-koji mould of comparative example, yeast bacterial content subtract
It is few, reduce the increase of milk composition in sow colostrum.
Experimental example 4
Control group piglet 10 are chosen in experimental example 2, and 1 piglet of embodiment 10 detects the physiochemical indice in piglet.
Influence of the 4 ganoderma lucidum residue fermented feed of table to piglet blood index
| Control group | Test group | |
| ALT glutamic-pyruvic transaminase (U/L) | 60.24±9.13 | 55.18±6.17 |
| AST glutamic-oxalacetic transaminease (U/L) | 45.17±4.19 | 37.96±6.14 |
| ALP alkaline phosphatase (U/L) | 163.74±28.11 | 164.51±20.55 |
| TP total protein (g/L) | 65.11±1.96 | 68.24±1.89 |
| GLO globulin (g/L) | 38.11±1.54 | 39.24±0.97 |
| A/G Archon ratio | 0.70±0.05 | 0.73±0.03 |
| TG triglycerides (mmol/L) | 0.42±0.02 | 0.37±0.03 |
| GLU glucose (mmol/L) | 4.71±0.41 | 4.33±0.15 |
The indexs such as immunoglobulin, alkaline phosphatase are able to reflect out whether body is healthy, and whether body is normal in physiochemical indice
Growth and development, the glutamic-pyruvic transaminase of test group, glutamic-oxalacetic transaminease, alkaline phosphatase, glucose, content of triglyceride are slightly below pair
According to group；And total protein, globulin and the albumin of test group are slightly above control group.Serum glutamic pyruvic transminase and glutamic-oxalacetic transaminease with
Liver function is closely related with cardiac function, and in certain negative correlation, their the higher reflection of level is both internal organs damage
That hurts is even more serious；And immune protein is directly associated with the immune function of body itself in serum, can also reflect lymphocyte
The ability of immune protein is generated with phagocyte, and then affects metabolism and the disease-fighting capabilities of entire body, is thus judged
The health status of body.
Claims (7)
1. a kind of preparation method of ganoderma lucidum slag fermentation material, which is characterized in that the ganoderma lucidum slag fermentation material includes: spirit by weight
3 ~ 10 parts of sesame slag, 5 ~ 13 parts of corn, 2 ~ 7 parts of wheat bran, 4 ~ 14 parts of glucose, 0.5 ~ 1 part of probiotics；
The preparation method includes the following steps:
S1. it pre-processes: by the cleaning of ganoderma lucidum slag, sterilizing, crushing, control water content is 50% ~ 70%, while crush maize is spare；
S2. it configures fermentation liquid: lactic acid bacteria, saccharomycete, bacillus subtilis, lichens bacillus subtilis, black-koji mould is configured
At probiotics, weight ratio 1:1:1:1:1 is added the water that temperature is 20 ~ 30 DEG C, fermentation liquid is made after mixing evenly；
S3. it ferments: after smashed ganoderma lucidum slag, corn flour and wheat bran are mixed, being added in fermentation liquid, while adding Portugal
Grape sugar and suitable quantity of water, make moisture content 45% ~ 65%, and after being sealed by fermentation 40 ~ 50h, drying obtains the spirit that moisture content is 8 ~ 12%
Sesame slag fermentation material.
2. the preparation method of ganoderma lucidum slag fermentation material according to claim 1, which is characterized in that the raw material wraps by weight
It includes: 5 ~ 8 parts of ganoderma lucidum slag, 8 ~ 11 parts of corn, 3 ~ 6 parts of wheat bran, 7 ~ 10 parts of glucose, 0.7 ~ 0.9 part of probiotics.
3. the preparation method of ganoderma lucidum slag fermentation material according to claim 1, which is characterized in that the ganoderma lucidum slag is ganoderma lucidum through water
Formulation extracts gained after polysaccharide.
4. the preparation method of ganoderma lucidum slag fermentation material according to claim 1, which is characterized in that be sealed by fermentation in the step S3
Environment be humidity 50% ~ 70%, 40 ~ 60 DEG C of temperature.
5. the ganoderma lucidum slag fermentation material that the preparation method of ganoderma lucidum slag fermentation material described in Claims 1-4 is prepared.
6. ganoderma lucidum slag fermentation material described in claim 5 is preparing the application in agent of feed for nursing sow additive.
7. applying according to claim 6, which is characterized in that the ganoderma lucidum slag fermentation material is added to milking sow conventional feed
In weight percent be 1 ~ 7%.
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