CN113956998A - Method for preparing thermophilic deodorizing city domestic garbage treating microbial inoculum and application thereof - Google Patents
Method for preparing thermophilic deodorizing city domestic garbage treating microbial inoculum and application thereof Download PDFInfo
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- 230000001877 deodorizing effect Effects 0.000 title claims description 10
- 238000000034 method Methods 0.000 title claims description 8
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- 241001052560 Thallis Species 0.000 claims abstract description 18
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 15
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- 239000000463 material Substances 0.000 claims abstract description 13
- 239000002131 composite material Substances 0.000 claims abstract description 8
- 238000004332 deodorization Methods 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims description 41
- 241000186361 Actinobacteria <class> Species 0.000 claims description 39
- 241000193749 Bacillus coagulans Species 0.000 claims description 39
- 244000063299 Bacillus subtilis Species 0.000 claims description 39
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- 229940054340 bacillus coagulans Drugs 0.000 claims description 39
- 238000009630 liquid culture Methods 0.000 claims description 37
- 241000894006 Bacteria Species 0.000 claims description 36
- 241000233866 Fungi Species 0.000 claims description 24
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 15
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 244000005700 microbiome Species 0.000 claims description 11
- 230000000593 degrading effect Effects 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 239000010791 domestic waste Substances 0.000 claims description 6
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- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
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- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
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- 238000010438 heat treatment Methods 0.000 claims description 3
- 235000020232 peanut Nutrition 0.000 claims description 3
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Abstract
The invention discloses a thermophilic deodorization urban domestic garbage treatment microbial inoculum, which consists of composite thalli and carrier auxiliary materials, wherein the composite thalli is formed by basic treatment thalli alone or formed by the basic treatment thalli and functional strains together; the high-temperature-resistant thermophilic waste treatment microbial inoculum disclosed by the invention has better removal rate of ammonia nitrogen and COD (chemical oxygen demand) in landfill leachate and better cellulose enzyme production activity than the common compound microbial inoculum sold in the market under the high-temperature state of 70 ℃; the test result of the outdoor simulation reactor also shows that the temperature of the center of the garbage rises faster, the concentration of ammonia nitrogen and COD in the garbage percolate is lower, and the settlement rate of the garbage after the reaction is finished is higher.
Description
Technical Field
The invention belongs to the technical field of garbage, and particularly relates to a preparation method and application of a thermophilic deodorization urban domestic garbage treatment microbial inoculum.
Background
The treatment mode of domestic garbage in China is mainly sanitary landfill, the generation amount of the domestic garbage is gradually increased year by year along with the gradual advance of the industrialization and the urbanization in recent years, and the problems of a garbage landfill site are gradually highlighted, such as long stabilization time of the landfill site, continuous generation of garbage percolate with high pollution intensity, long-term emission of malodorous gas and the like. Research finds that the action of microorganisms is crucial in the stabilization process of the landfill, and the microorganisms are main decomposers of organic substances in the landfill, but under the complex environmental influence of the landfill, the efficiency of decomposing solid garbage only by indigenous microorganisms in the landfill is low, so that the degradation speed of the garbage is low, the pollution concentration is high, and the stabilization time of the landfill is long. The biological strengthening technology developed gradually in the 80's of the last century can enhance the degradation of various refractory substances and improve the efficiency of the original biological treatment system by introducing microorganisms with specific degradation functions. By utilizing the technology, the prior people have beneficially explored the treatment aspect of the solid garbage, and by putting special microorganisms capable of degrading COD and cellulose into the garbage landfill, the decomposition efficiency of the solid garbage is improved, and the stabilization process of the landfill is accelerated. However, in the research, the decomposition of the microorganisms on the garbage is closely related to the temperature, the efficiency of the microorganisms can achieve the best effect when the temperature of the simulation reactor is controlled to be 40 ℃, the temperature of the garbage center is up to 70 ℃, the decomposition efficiency of the microorganisms on the garbage is greatly reduced, and the treatment of the common microbial inoculum on the solid garbage is limited.
An effective solution to the problems in the related art has not been proposed yet.
Disclosure of Invention
In order to achieve the above object, the technical scheme adopted by the invention is that the invention provides a thermophilic deodorization urban domestic garbage treatment microbial inoculum, which comprises composite thalli and carrier auxiliary materials, wherein the composite thalli is composed of basic treatment thalli alone or both the basic treatment thalli and functional strains;
the basic processing thallus consists of bacillus subtilis, bacillus coagulans, actinomycetes, mould and saccharomyces cerevisiae, and the carrier auxiliary material is formed by mixing components for providing carbon sources and nitrogen sources required by microorganisms and a loose and breathable carrier.
Preferably, in the basic treatment thallus, the bacillus subtilis accounts for 30-65% of the total viable count, the bacillus coagulans accounts for 20-65% of the total viable count, the actinomycetes accounts for 10-25% of the total viable count, the mould accounts for 40-60% of the total viable count, and the saccharomyces cerevisiae accounts for 5-50% of the total viable count.
Preferably, in the basic treatment thalli, the bacillus subtilis accounts for 40-55% of the total viable count, the bacillus coagulans accounts for 30-45% of the total viable count, and the saccharomyces cerevisiae accounts for 15-30% of the total viable count.
Preferably, the carrier auxiliary material is formed by mixing soybean meal, corn flour, sugar powder and wood shavings in a weight ratio of (2-5) to (2-3) to (1-3) to (0.5-1).
Preferably, the carrier auxiliary material uses rice bran and peanut bran as a carbon source and a nitrogen source.
Preferably, the treatment microbial inoculum can be applied to municipal domestic waste.
A method for preparing a thermophilic deodorization urban domestic garbage treatment microbial inoculum comprises the following steps:
s1, collecting strains; firstly, respectively collecting bacillus subtilis, bacillus coagulans, actinomycetes and mould; taking a proper amount of saccharomyces cerevisiae for standby;
s2, strain enrichment and strain domestication; inoculating the single pure colony obtained in the step 1 to a bacteria liquid culture medium, an actinomycete liquid culture medium and a mould liquid culture medium correspondingly, performing shake culture at 28 ℃ and 120r/min by a shaking table, respectively inoculating the bacteria liquid to the liquid culture medium containing 20 wt% of landfill leachate for 3-5 days after the bacteria liquid OD600nm reaches 0.7 +/-0.05, then inoculating to the liquid culture medium containing 40 wt% of the landfill leachate for 4 days, then inoculating to the liquid culture medium containing 60 wt% of the landfill leachate for 4 days, then inoculating to the liquid culture medium containing 80 wt% of the landfill leachate for 4 days, and finally inoculating to the liquid culture medium containing 100 wt% of the landfill leachate for 4 days to obtain the tolerable bacillus subtilis, bacillus coagulans, actinomycetes and mould.
S3, primary screening of the strain; respectively carrying out enrichment culture on domesticated and tolerable bacillus subtilis, bacillus coagulans, actinomycetes and mould fungi in a liquid culture medium until the bacterial liquid OD600nm is 0.7 +/-0.05, respectively inoculating the bacterial liquids of the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi to the landfill leachate according to the volume ratio of 1: 20, and culturing for 48 hours to obtain the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi capable of removing ammonia nitrogen and COD;
s4, thermophilic domestication; respectively inoculating the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi capable of removing ammonia nitrogen and COD and cellulose degrading bacteria to a culture medium, and performing high-temperature acclimation on strains by adopting a gradient cooling method, wherein the strains are firstly cultured at 28 ℃ for 2-3 days, the culture temperature is raised by 3 ℃ for 5-7 days, the heating culture operation is repeatedly performed, and when the temperature is raised to 60-70 ℃, high-temperature-resistant and thermophilic bacillus subtilis, bacillus coagulans, actinomycetes and mould fungi capable of removing ammonia nitrogen and COD and high-temperature-resistant cellulose degrading bacteria are obtained;
s5, preparing a treatment microbial inoculum; respectively inoculating the high-temperature-resistant and thermophilic bacillus subtilis, bacillus coagulans, actinomycetes, mould fungi and high-temperature-resistant cellulose degrading bacteria into a liquid culture medium, an actinomycetes liquid culture medium and a mould fungi liquid culture medium for activated culture, and carrying out compatibility according to the volume fractions that the bacillus subtilis accounts for 30 percent of the total viable bacteria, the bacillus coagulans accounts for 20 percent of the total viable bacteria, the actinomycetes accounts for 8 percent of the total viable bacteria, the mould fungi accounts for 32 percent of the total viable bacteria and the saccharomyces cerevisiae accounts for 10 percent of the total viable bacteria to obtain the treatment microbial inoculum.
Preferably, the liquid medium is a medium commonly used in the prior art for culturing bacillus subtilis, bacillus coagulans, actinomycetes, and molds and cellulose-degrading bacteria resistant to high temperature.
Has the advantages that: compared with the prior art, the high-temperature-resistant thermophilic waste treatment microbial inoculum has the advantages and positive effects that the removal rate of ammonia nitrogen and COD in landfill leachate and the activity of cellulose enzyme production are superior to those of the common compound microbial inoculum sold in the market under the high-temperature state of 70 ℃ and the growth condition of the microbial inoculum; the test result of the outdoor simulation reactor also shows that the temperature of the center of the garbage rises faster, the concentration of ammonia nitrogen and COD in the garbage percolate is lower, and the settlement rate of the garbage after the reaction is finished is higher.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive labor.
Fig. 1 is a comparison graph of the treatment effect of the thermophilic deodorizing urban domestic waste treatment microbial inoculum provided by the embodiment and the treatment effect of the commercial microbial inoculum at different temperatures.
In the figure, a broken line A is a broken line diagram of the effect of the treatment microbial inoculum on garbage treatment provided by the invention;
the broken line B is a broken line diagram of the effect of the commercial microbial inoculum on garbage treatment provided by the invention.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Examples
In order that the above objects, features and advantages of the present invention can be more clearly understood, the present invention will be further described with reference to the accompanying drawings and examples. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and thus the present invention is not limited to the specific embodiments of the present disclosure.
The embodiment can be seen from the attached drawings of the specification, the scheme provides a thermophilic deodorization urban domestic garbage treatment microbial inoculum which consists of composite thalli and carrier auxiliary materials, wherein the composite thalli is composed of basic treatment thalli alone or both the basic treatment thalli and functional strains;
the basic processing thallus consists of bacillus subtilis, bacillus coagulans, actinomycetes, mould and saccharomyces cerevisiae, and the carrier auxiliary material is formed by mixing components for providing carbon sources and nitrogen sources required by microorganisms and a loose and breathable carrier.
Preferably, in the basic treatment thallus, the bacillus subtilis accounts for 30-65% of the total viable count, the bacillus coagulans accounts for 20-65% of the total viable count, the actinomycetes accounts for 10-25% of the total viable count, the mould accounts for 40-60% of the total viable count, and the saccharomyces cerevisiae accounts for 5-50% of the total viable count.
Preferably, in the basic treatment thalli, the bacillus subtilis accounts for 40-55% of the total viable count, the bacillus coagulans accounts for 30-45% of the total viable count, and the saccharomyces cerevisiae accounts for 15-30% of the total viable count.
Preferably, the carrier auxiliary material is formed by mixing soybean meal, corn flour, sugar powder and wood shavings in a weight ratio of (2-5) to (2-3) to (1-3) to (0.5-1).
Preferably, the carrier auxiliary material uses rice bran and peanut bran as a carbon source and a nitrogen source.
Preferably, the treatment microbial inoculum can be applied to municipal domestic waste.
A method for preparing a thermophilic deodorization urban domestic garbage treatment microbial inoculum comprises the following steps:
s1, collecting strains; firstly, respectively collecting bacillus subtilis, bacillus coagulans, actinomycetes and mould; taking a proper amount of saccharomyces cerevisiae for standby;
s2, strain enrichment and strain domestication; inoculating the single pure colony obtained in the step 1 to a bacteria liquid culture medium, an actinomycete liquid culture medium and a mould liquid culture medium correspondingly, performing shake culture at 28 ℃ and 120r/min by a shaking table, respectively inoculating the bacteria liquid to the liquid culture medium containing 20 wt% of landfill leachate for 3-5 days after the bacteria liquid OD600nm reaches 0.7 +/-0.05, then inoculating to the liquid culture medium containing 40 wt% of the landfill leachate for 4 days, then inoculating to the liquid culture medium containing 60 wt% of the landfill leachate for 4 days, then inoculating to the liquid culture medium containing 80 wt% of the landfill leachate for 4 days, and finally inoculating to the liquid culture medium containing 100 wt% of the landfill leachate for 4 days to obtain the tolerable bacillus subtilis, bacillus coagulans, actinomycetes and mould.
S3, primary screening of the strain; respectively carrying out enrichment culture on domesticated and tolerable bacillus subtilis, bacillus coagulans, actinomycetes and mould fungi in a liquid culture medium until the bacterial liquid OD600nm is 0.7 +/-0.05, respectively inoculating the bacterial liquids of the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi to the landfill leachate according to the volume ratio of 1: 20, and culturing for 48 hours to obtain the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi capable of removing ammonia nitrogen and COD;
s4, thermophilic domestication; respectively inoculating the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi capable of removing ammonia nitrogen and COD and cellulose degrading bacteria to a culture medium, and performing high-temperature acclimation on strains by adopting a gradient cooling method, wherein the strains are firstly cultured at 28 ℃ for 2-3 days, the culture temperature is raised by 3 ℃ for 5-7 days, the heating culture operation is repeatedly performed, and when the temperature is raised to 60-70 ℃, high-temperature-resistant and thermophilic bacillus subtilis, bacillus coagulans, actinomycetes and mould fungi capable of removing ammonia nitrogen and COD and high-temperature-resistant cellulose degrading bacteria are obtained;
s5, preparing a treatment microbial inoculum; respectively inoculating the high-temperature-resistant and thermophilic bacillus subtilis, bacillus coagulans, actinomycetes, mould fungi and high-temperature-resistant cellulose degrading bacteria into a liquid culture medium, an actinomycetes liquid culture medium and a mould fungi liquid culture medium for activated culture, and carrying out compatibility according to the volume fractions that the bacillus subtilis accounts for 30 percent of the total viable bacteria, the bacillus coagulans accounts for 20 percent of the total viable bacteria, the actinomycetes accounts for 8 percent of the total viable bacteria, the mould fungi accounts for 32 percent of the total viable bacteria and the saccharomyces cerevisiae accounts for 10 percent of the total viable bacteria to obtain the treatment microbial inoculum.
Preferably, the liquid medium is a medium commonly used in the prior art for culturing bacillus subtilis, bacillus coagulans, actinomycetes, and molds and cellulose-degrading bacteria resistant to high temperature.
The high-temperature-resistant thermophilic waste treatment microbial inoculum disclosed by the invention has better removal rate of ammonia nitrogen and COD (chemical oxygen demand) in landfill leachate and better cellulose enzyme production activity than the common compound microbial inoculum sold in the market under the high-temperature state of 70 ℃; the test result of the outdoor simulation reactor also shows that the temperature of the center of the garbage rises faster, the concentration of ammonia nitrogen and COD in the garbage percolate is lower, and the settlement rate of the garbage after the reaction is finished is higher.
The above description is only a preferred embodiment of the present invention, and not intended to limit the present invention in other forms, and any person skilled in the art may apply the above modifications or changes to the equivalent embodiments with equivalent changes, without departing from the technical spirit of the present invention, and any simple modification, equivalent change and change made to the above embodiments according to the technical spirit of the present invention still belong to the protection scope of the technical spirit of the present invention.
Claims (8)
1. A thermophilic deodorization city domestic garbage treatment microbial inoculum is characterized in that the treatment microbial inoculum consists of composite thalli and carrier auxiliary materials, wherein the composite thalli is composed of basic treatment thalli alone or the basic treatment thalli and functional strains together;
the basic processing thallus consists of bacillus subtilis, bacillus coagulans, actinomycetes, mould and saccharomyces cerevisiae, and the carrier auxiliary material is formed by mixing components for providing carbon sources and nitrogen sources required by microorganisms and a loose and breathable carrier.
2. The thermophilic deodorizing city domestic garbage treatment microbial inoculum according to claim 1, wherein in the basic treatment microbial inoculum, bacillus subtilis accounts for 30-65% of the total viable count, bacillus coagulans accounts for 20-65% of the total viable count, actinomycetes accounts for 10-25% of the total viable count, mold accounts for 40-60% of the total viable count, and saccharomyces cerevisiae accounts for 5-50% of the total viable count.
3. The thermophilic deodorizing city domestic garbage treatment microbial inoculum according to claim 1, wherein in the basic treatment microbial inoculum, bacillus subtilis accounts for 40-55% of the total viable count, bacillus coagulans accounts for 30-45% of the total viable count, and saccharomyces cerevisiae accounts for 15-30% of the total viable count.
4. The thermophilic deodorizing microbial inoculum for treating urban domestic garbage according to claim 1, wherein the carrier auxiliary materials are prepared by mixing soybean meal, corn meal, powdered sugar and wood shavings in a weight ratio of (2-5) to (2-3) to (1-3) to (0.5-1).
5. The thermophilic deodorizing urban domestic garbage treatment microbial inoculum according to claim 1, wherein the carrier auxiliary materials use rice bran and peanut bran as carbon source and nitrogen source.
6. The thermophilic deodorizing urban domestic waste treatment bacterial agent according to any one of claims 1 to 5, wherein said treatment bacterial agent can be applied to urban domestic waste.
7. A method for preparing a thermophilic deodorizing city domestic waste treatment microbial inoculum according to any one of claims 1 to 5, characterized by comprising the following steps:
s1, collecting strains; firstly, respectively collecting bacillus subtilis, bacillus coagulans, actinomycetes and mould; taking a proper amount of saccharomyces cerevisiae for standby;
s2, strain enrichment and strain domestication; inoculating the single pure colony obtained in the step 1 to a bacteria liquid culture medium, an actinomycete liquid culture medium and a mould liquid culture medium correspondingly, performing shake culture at 28 ℃ and 120r/min by a shaking table, respectively inoculating the bacteria liquid to the liquid culture medium containing 20 wt% of landfill leachate for 3-5 days after the bacteria liquid OD600nm reaches 0.7 +/-0.05, then inoculating to the liquid culture medium containing 40 wt% of the landfill leachate for 4 days, then inoculating to the liquid culture medium containing 60 wt% of the landfill leachate for 4 days, then inoculating to the liquid culture medium containing 80 wt% of the landfill leachate for 4 days, and finally inoculating to the liquid culture medium containing 100 wt% of the landfill leachate for 4 days to obtain the tolerable bacillus subtilis, bacillus coagulans, actinomycetes and mould.
S3, primary screening of the strain; respectively carrying out enrichment culture on domesticated and tolerable bacillus subtilis, bacillus coagulans, actinomycetes and mould fungi in a liquid culture medium until the bacterial liquid OD600nm is 0.7 +/-0.05, respectively inoculating the bacterial liquids of the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi to the landfill leachate according to the volume ratio of 1: 20, and culturing for 48 hours to obtain the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi capable of removing ammonia nitrogen and COD;
s4, thermophilic domestication; respectively inoculating the bacillus subtilis, the bacillus coagulans, the actinomycetes and the mould fungi capable of removing ammonia nitrogen and COD and cellulose degrading bacteria to a culture medium, and performing high-temperature acclimation on strains by adopting a gradient cooling method, wherein the strains are firstly cultured at 28 ℃ for 2-3 days, the culture temperature is raised by 3 ℃ for 5-7 days, the heating culture operation is repeatedly performed, and when the temperature is raised to 60-70 ℃, high-temperature-resistant and thermophilic bacillus subtilis, bacillus coagulans, actinomycetes and mould fungi capable of removing ammonia nitrogen and COD and high-temperature-resistant cellulose degrading bacteria are obtained;
s5, preparing a treatment microbial inoculum; respectively inoculating the high-temperature-resistant and thermophilic bacillus subtilis, bacillus coagulans, actinomycetes, mould fungi and high-temperature-resistant cellulose degrading bacteria into a liquid culture medium, an actinomycetes liquid culture medium and a mould fungi liquid culture medium for activated culture, and carrying out compatibility according to the volume fractions that the bacillus subtilis accounts for 30 percent of the total viable bacteria, the bacillus coagulans accounts for 20 percent of the total viable bacteria, the actinomycetes accounts for 8 percent of the total viable bacteria, the mould fungi accounts for 32 percent of the total viable bacteria and the saccharomyces cerevisiae accounts for 10 percent of the total viable bacteria to obtain the treatment microbial inoculum.
8. The method for preparing a thermophilic deodorizing city domestic garbage treatment microbial inoculum and the application thereof according to claim 1, wherein the liquid culture medium is a culture medium commonly used in the prior art for culturing bacillus subtilis, bacillus coagulans, actinomycetes, mold and high temperature resistant cellulose degradation bacteria.
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