WO2016182364A1 - Soil conditioner prepared using composite microorganism seed and method for preparing same - Google Patents

Abstract

The present invention relates to a soil conditioner prepared using a composite microorganism seed and a method for preparing the same, wherein the soil conditioner is completed by culturing a composite seed of a plurality of microorganisms including Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae using a culture medium, which is formed by mixing lysine, propolis, syrup, and water, and then filtering the cultured liquid material, of which the pH value is measured within a predetermined range.

Description

Soil modifier produced using the complex microbial spawn and its manufacturing method

The present invention relates to a method for producing a soil improving agent using a spawn comprising a plurality of microorganisms and a soil improving agent produced by the method.

Soil is a substance deposited on the surface of the earth. Most of the soil is rock weathering. Mixtures and chemical reaction products that are broken by large or small particles due to oxygen, water, and thermal reactions of rocks exposed to or near the surface. It is composed of organic matter. In agriculture and forestry, soil is defined as the nutrients of plants, the storage and regulation of moisture, the release, and the support of plants.

Many harmful substances produced by human activities are absorbed into the soil, resulting in soil pollution, which causes the soil to lose its function as an environmental component. Soil pollution is largely due to the use of underground resources, where minerals in rocks accumulate on the surface, Pesticides accumulate synthetic organochlorine compounds or alkyl mercury compounds, and are also caused by acid rain from industrial complexes and urban soot gases, food packaging waste, and waste from facility livestock. In addition, inorganic components such as heavy metals released by industrialization contaminate cropland soils and cause growth disorders of crops, causing harm to humans and livestock during the food chain. One of the alternatives used to solve such soil pollution is soil improver, which is used to improve the physical, chemical and biological properties of soil, to improve the physical properties by coagulation or granulation of soil. Also called a disinfectant.

On the other hand, the prior art disclosed in Republic of Korea Patent No. 10-1259416 is 0.001 ~ 0.02 parts by weight of mixed microorganisms (BM-S-1), 2-5 parts by weight of fermented rice bran, 3-7 weight of fermented rice hull powder 5 to 10 parts by weight of shiitake mushroom mycelium powder, 3 to 5 parts by weight of peat moss powder, 1 to 3 parts by weight of molasses, 2 to 5 parts by weight of trehalose, and 0.5 to 2 parts by weight of bamboo activated charcoal powder by mixing water in total 100 A composite microbial liquid preparation is prepared so as to be part by weight, and a mixed raw material is mixed with the mixed microbial liquid to prepare a mixed raw material, and then, the microorganism is inoculated and cultured to prepare a soil improving agent. This prior art has a disadvantage in that the process is complicated and the manufacturing time is long because the mixed raw material manufacturing step is complicated and includes a high temperature inoculation step, a drying step, and a molding step.

As described above, the soil improver produced using microorganisms such as the prior art disclosed in the above-described patent has an effect as a soil improver because the number of beneficial bacteria included in the soil improver is not sufficient, although the manufacturing process is complicated and the production time is long. There was a problem falling.

It is to provide a method for producing an environmentally friendly soil improver that can increase the number of beneficial bacteria in a short time through a simple manufacturing process. It is also to provide a soil improver produced by the method.

In the method for producing a soil improver using a complex microbial seed according to an aspect of the present invention, Bacillus subtilis , Lactobacillus plantarum , and Saccharomyces cerevisiae (Saccharomyces cerevisiae) Injecting the lysine, propolis, molasses, and water into the culture vessel with a plurality of microorganisms including the seed, the culture medium formed by mixing the lysine, propolis, molasses and water added to the culture vessel A culture step of culturing the complex microorganism spawn put into the culture vessel, a measurement step of measuring the pH value of the liquid material cultured in the culture step, and by filtering the liquid material measured the pH value within a predetermined range in the measurement step soil A filtration step may be included to complete the modifier.

The composite microbial inoculum is Lactobacillus casei (Lactobacillus casei), Lactobacillus Ecija FIG filler's (Lactobacillus acidophilus), rock Fig Bacillus flow Pocono stock (Lactobacillus leuconostoc), Lactobacillus brevis (Lactobacillus brevis), Streptococcus faecalis (Streptococcus faecalis) , Bacillus putrificus , Bacillus cereus , Pseudomonas fluorescens , and Aspergillus oryzae . . The complex microbial spawn may further include at least one of green sulfur bacteria, red sulfur bacteria, and red non-sulfur bacteria.

The feeding step may be added to the composite microbial spawn 0.7 ~ 0.9 parts by weight, lysine 0.1 ~ 0.3 parts by weight, propolis 0.1 ~ 0.3 parts by weight and molasses 4.5 ~ 6.5 parts by weight based on 100 parts by weight of water, the culture step Complex microorganism spawn having 0.7 to 0.9 parts by weight based on 100 parts by weight of the water by using a culture medium mixed with 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses based on 100 parts by weight Can be cultured.

The culturing step may be performed by aeration while simultaneously stirring intermittently with 0.7 to 0.9 parts by weight of the complex microorganism spawn, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses. Complex microbial spawn can be cultured. The culturing step may perform the intermittent agitation and aeration by repeating the process of stopping the agitation and aeration for a second time longer than the first time after the agitation and aeration for the first time. The culturing step may be carried out the intermittent stirring and aeration while maintaining the temperature of the culture solution in the range 35 ~ 45 ℃.

The filtration step may complete the soil improver by filtering the liquid material measured in the range of pH value of 3 to 4.5 in the measuring step. In the filtration step, the liquid material corresponding to the soil improver may be discharged from the filter by filtering the liquid material whose pH value is measured within the predetermined range in at least one or more times using a filter having a pore size of 200 mesh. have.

A soil modifier according to another aspect of the present invention is prepared by a method for producing a soil modifier using the complex microorganism.

Lysine, propolis, molasses and water are mixed using a defined culture medium Basil Russ subtilis (Bacillus subtilis), Lactobacillus Plan tareom (Lactobacillus plantarum), and a saccharide, by culturing a plurality of the composite seed microorganism containing the busy MRS serenity (Saccharomyces cerevisiae) can be produced a significant increase in the numbers of microorganisms soil conditioner.

Complex microorganism having 0.7 to 0.9 parts by weight based on 100 parts by weight of water using a culture medium mixed with 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses, based on 100 parts by weight of water. By culturing the spawn, it is possible to prepare a soil improver in which the population of microorganisms is greatly increased in a short time.

Soil modifier according to the present invention can be prepared by a simple process consisting of a water purification step 10, the input step 20, the culture step 30, the measurement step 40, the filtration step 50 and the like. In addition, since any process in the manufacturing process of the soil improver does not require chemicals can provide an environmentally friendly soil improver.

1 is a flow chart of a method for producing a soil improver according to an embodiment of the present invention.

Hereinafter, the content of the present invention in more detail as follows.

The present invention is a Bacillus subtilis (Bacillus Subtilis), Lactobacillus plantarum (Lactobacillus plantarum), Sakae using a culture medium containing lysine (Lysine), Propolis (Propolis), molasses (Molasses), and Water (Water) The present invention relates to a soil improver prepared by culturing a spawn of a plurality of microorganisms including Saccharomyces cerevisiae and filtering the cultured liquid material.

The soil improving agent according to the present invention mixes 0.7 to 0.9 parts by weight of complex microorganism spawn, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis, and 4.5 to 6.5 parts by weight of molasses, based on 100 parts by weight of water, and the culture temperature. Incubate by intermittent stirring and aeration while maintaining in the range of 35 ~ 45 ℃.

Bacillus subtilis, Lactobacillus plantarum, Saccharomyces cerevisiae, including several Bacillus subtilis used in the preparation of soil improvers, lysine, propolis , Molasses, and the description of the nature and use of water will be described later.

Bacillus Subtilis is called Bacillus subtilis (a bacterium that is non-toxic and forms spores). A representative bacterium belonging to the genus Bacillus, widely distributed in nature such as soil, hay, dust, etc., and is an aerobic microorganism. Bacillus subtilis (Bacillus Subtilis) through the metabolic activity to produce a large amount of beneficial enzymes and biologically active materials, such as to feed the microorganisms present in the soil and plants planted in the soil to help growth. In addition, by secreting a variety of antibiotics to prevent various diseases caused by plants.

Lactobacillus plantarum (Lactobacillus plantarum) is a salt-resistant lactic acid bacteria (bacteria that break down sugars such as glucose to produce lactic acid), and corresponds to the anaerobic anaerobic. The Lactobacillus plantarum (Lactobacillus plantarum) is to produce organic acids as a by-product, to improve the soil by inhibiting the growth of other bacteria, and to produce a lactolin (lactolin), one of the excellent antibacterial substances to prevent pests.

In MRS celebrity busy as Saccharomyces (Saccharomyces cerevisiae), most of the yeast used in brewing, as well as, baker's yeast or brewer's yeast as a representative yeast belonging to Ascomycetes (bacteria create an ascus spores to form the ascus by sexual reproduction in fungi) Is the latest of this species. Saccharomyces cerevisiae promotes the activity of soil microorganisms by supplying useful bioactive substances such as amino acids, vitamins, minerals, and digestive enzymes through metabolic activities, and decomposing organic matter using microorganisms through fermentation. Improve soil by promoting the activity of soil microorganisms.

Complex microorganism spawn of the present invention can be collected from soybean, molasses, rice and the like, in addition to the aforementioned Bacillus Subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae Lactobacillus casei (Lactobacillus casei), Lactobacillus Ecija FIG filler's, Bacillus Pew tree (Lactobacillus acidophilus), Lactobacillus flow Pocono stock (Lactobacillus leuconostoc), Lactobacillus brevis (Lactobacillus brevis), Streptococcus faecalis (Streptococcus faecalis) It contains various spawns such as Bacillus putrificus, Bacillus cereus, Pseudomonas fluorescens and Aspergillus oryzae .

In addition, the complex microorganism spawn of the present invention may further include photosynthetic bacteria such as green sulfur bacteria, red sulfur bacteria, and red non-sulfur bacteria together with the aforementioned microorganisms. Photosynthetic bacteria are carbon-assimilated bacteria that use light energy to photosynthesize carbon dioxide and hydrogen compounds in bacteriocholorophylls instead of chlorophyll. Bacteriochlorophyll is a pigment of photosynthetic bacteria, and there are several pigments such as a and b. The clear main pigment of the structure is called bacteriochlorophyll a, and its structure is called chlorophyll a (C ₅₅ H ₇₂ MgN ₄ O _5). 1 out of 4 pyrrole nuclei is reduced form (C ₅₅ H ₇₄ MgN ₄ 0 ₅ ). These bacteriochlorophylls exhibit major absorption peaks at about 357.5 nanometers and about 770 nanometers in alcohol solutions, are bound to proteins within cells, and exhibit absorption peaks at about 380, 800, 850, 870, and 890 nanometers. A light portion of 890 nanometers is used for photosynthesis. In particular, red non-sulfur bacteria have photosynthesis with lower fatty acids from organic pollutants and grow well under both anaerobic and aerobic conditions.

These photosynthetic bacteria are added to the soil improving agent prepared according to the present invention to improve the soil by acting such as carbon assimilation, nitrogen fixation, and odor removal. The above-mentioned carbon assimilation refers to the action of any bacteria to make an organic carbon compound with carbon dioxide and water, and the organic carbon compound produced by carbon assimilation is supplied to the plant, and the supplied organic carbon compound is used as the energy source of the plant and the soil. Help the growth of plants planted in. Photosynthetic bacteria supply nitrogen to soil and plants planted in the soil with excellent nitrogen fixation ability, produce excellent antimicrobial and antiviral substances through metabolic activities, inhibit the growth of harmful bacteria, and hydrogen sulfide, which is the cause of odor of soil Decompose odor by decomposing ammonia, acetic acid and amine materials. In addition, since it removes harmful substances and inhibits the growth of harmful bacteria, it serves to increase the activity of other microorganisms present in the soil improver.

However, since photosynthetic bacteria have a high lytic ability to destroy cell walls of bacteria, they may interfere with the growth of harmful bacteria as well as beneficial bacteria, and there is a problem of increasing sulfides in the soil by the sulfate action that produces sulfates or esters. Care should be taken to avoid overuse as it may be present. Generally, the microbial strains are mixed at a ratio of 1 to 40% by weight, and the number of microbial strains is known to be about 10 ² to 10 ³ cfu / g.

Complex microorganism spawn of the present invention is 0.7 to 0.9 parts by weight with respect to 100 parts by weight of water, when less than 0.7 parts by weight, because the microorganisms necessary for the cultivation is not sufficiently supplied, the growth of the microorganism population through the cultivation of the soil improver The number of beneficial bacteria can be reduced, and if it exceeds 0.9 parts by weight, the proportion of microorganisms will be higher than the ratio of lysine, propolis, molasses and water used for the culture of the microorganisms, making it difficult for the microorganisms to multiply through sufficient feeding activity. In addition, as the medium contained in the complex microbial spawn is excessively mixed, the culture solution may be contaminated by the byproduct.

Lysine is a basic amino acid that acts as a growth accelerator to promote growth and development, also called lysine. In the present invention, lysine is used as a culture feed for complex microbial spawns such as Bacillus Subtilis, Lactobacillus plantarum, Saccharomyces cerevisiae, etc. Promotes the growth of beneficial bacteria population. Some of the remaining lysine used as a culture feed for the complex microbial spawn can remain in the soil improver to help the decomposition of dioxin, a harmful substance present in the soil, and L-lysine, one of the isomers of lysine. Increasing resistance to disease helps prevent plant pests.

When lysine used in the present invention is less than 0.1 parts by weight to 0.1 to 0.3 parts by weight with respect to 100 parts by weight of water, the growth efficiency of the microorganisms may not be sufficiently supplied due to the feeding of the complex microbial spawn in the culture step, and remains in the soil improver. If it is difficult to remove the harmful substances in the soil and the amount of lysine exceeds 0.3 parts by weight, the growth efficiency of the microorganisms may be reduced due to the lack of space and breeding space of microorganisms in the culture.

Propolis is made by mixing bees and enzymes with resin such as honeybees extracted from various plants for their survival and breeding. Many, minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism, terpenes, etc. have an anticancer effect. In the present invention, it is used to feed the complex microbial spawn in the culturing step to help increase the number of beneficial bacteria population. Some of the remaining propolis used as a culture feed for the complex microbial spawn remain as a soil improver, inhibiting the growth of harmful bacteria in the soil and acting as an eco-friendly fungicide with excellent sterilization and antibacterial activity.

When the propolis used in the present invention is less than 0.1 to 0.3 parts by weight with respect to 100 parts by weight of water, the growth efficiency of the microorganisms may be lowered due to insufficient feeding of the complex microorganism spawn in the culture step, and absorbed into soil and plants. It may not be enough as a natural antibiotic, if the amount of propolis is 0.3 parts by weight or more, the microorganisms in the culture medium and the spawning space is insufficient to reduce the growth efficiency of the microorganisms due to excessive antibacterial activity as well as harmful bacteria Growth of beneficial bacteria can also be difficult.

Molasses are blackish syrupy liquids that are extracted from sugars in the sugar making process from sugar cane, sugar beet, etc. through the process of powdering, refining, and crystallization, and contains 20 to 30% water. The main ingredient is sugar. Molasses may be classified into sugar cane molasses, sugar beet molasses, corn molasses, citrus molasses, sorghum molasses, and wooden molasses. Molasses is a substance having various nutrients, such as dietary fiber, folic acid, protein, vitamins, and potassium, and is used as a culture feed of the complex microbial spawn in the present invention.

Molasses is a liquid viscous substance with a viscosity of 500 Pa · s and higher than 1 mPa · s of water (20 ° C. standard), so that molasses is separately dissolved at 40 ° C. and added to the culture vessel. In addition, glucose and brown sugar, etc. can be used as a culture feed for complex microbial spawns in place of molasses, but cost-effectiveness was obtained by using molasses, which is relatively easy to obtain and has a low cost burden.

Molasses used in the present invention is less than 4.5 parts by weight to 4.5 to 6.5 parts by weight with respect to 100 parts by weight of water. In the culturing stage, the microbial propagation efficiency may be reduced due to insufficient feeding of the culture microbial spawn and the molasses exceeds 6.5 parts by weight. Insufficient habitat and breeding space of microorganisms in culture media can reduce microbial growth efficiency The propagation efficiency of the microbial spawn may decrease.

In the present invention, all components except the complex microorganism spawn, lysine, propolis, and molasses are all composed of water, and water is used as a solvent for raw materials. Water has a specific heat that is large enough to control the temperature of thermophilic animals, so it is not sensitive to changes in temperature and other conditions and does not cause chemical reactions with other substances.

The soil improver according to the present invention is prepared by mixing 0.7 to 0.9 parts by weight of the complex microorganism spawn, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis, and 4.5 to 6.5 parts by weight of molasses. The mixing ratio of one component is an optimal condition for culturing the complex microbial spawn, and a preferred embodiment will be described below.

Hereinafter, with reference to the drawings will be described in detail a method for producing a soil improver using a complex microbial spawn according to an embodiment of the present invention.

1 is a flow chart of a method for producing a soil improver according to an embodiment of the present invention. Referring to FIG. 1, the method for preparing a soil improver according to the present embodiment includes a water purification step 10, an input step 20, a culture step 30, a measurement step 40, and a filtration step 50. .

The purification step 10 purifies the tap water to remove by-products such as chlorine from the tap water. In more detail, in the water purification step 10, tap water may be added to a heating vessel provided separately from the culture vessel, and purified by heating for 3 to 24 hours in a temperature range of 60 to 80 ° C. Tap water includes mixed bacteria other than chlorine (Cl), fluorine (F), calcium (Ca), magnesium (Mg), iron (Fe), other by-products, and certain beneficial bacteria. The purification step 10 purifies the tap water with water suitable for the growth of microorganisms by removing such chlorine, various bacteria, and other by-products from the tap water.

In the input step (20), lysine, together with a spawn of a plurality of microorganisms including Bacillus Subtilis, Lactobacillus plantarum, Saccharomyces cerevisiae, Propolis, molasses, and water are added to the culture vessel. According to this embodiment, in the input step 20, 0.7 to 0.9 parts by weight of the complex microorganism spawn, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses are added to 100 parts by weight of water. . This dosing step 20 follows a series of processes as follows. First, when the purified water is cooled in the purification step 10 and the water temperature reaches about 40 ° C., which is the optimum culture temperature of the microorganisms, water corresponding to about 80% of the total amount of water to be added to the culture vessel is added to the culture vessel. .

Subsequently, molasses dissolved at about 40 ° C. is added to the culture vessel, and then the complex microorganism spawn, lysine, and propolis are added to the culture vessel. As such, molasses is introduced before complex microbial spawn, lysine, and propolis because molasses has a high viscosity, and therefore, homogeneity between materials due to the unique viscosity of molasses when added later than complex microbial spawn, lysine, and propolis. This is because mixing may be disturbed. Finally, close the lid of the culture vessel after adding about 20% of water to the culture vessel until the space corresponding to about 85% of the culture vessel is filled. The space corresponding to about 15% of the culture vessel should be empty in order to provide a smooth oxygen supply to the culture mixture of lysine, propolis, molasses, and water. In order to precisely empty the space corresponding to about 15% of the culture vessel, water is added to the culture vessel in two portions as described above.

In the culturing step (30), Bacillus subtilis , Lactobacillus plantarum , and Saccharomyces cerevisiae , which were introduced into the culture vessel using a culture solution containing lysine, propolis, molasses, and water. A microorganism including a plurality of microorganisms including E. (Saccharomyces cerevisiae) is cultured. In the prior art, microbial spawn is cultured mainly by feeding molasses. As shown in Test Example 2 to be described later, when the lysine, propolis, molasses is added together compared to the case where only molasses, the population of microorganisms is greatly increased. According to this example, only by culturing the complex microbial spawn as described above only for about 72 hours, the number of microorganisms increased rapidly as shown in Test Example 1 to be described later.

In the culturing step (30) while maintaining the culture temperature within the range of 35 ~ 45 ℃ 0.7 to 0.9 parts by weight of the complex microorganism spawn, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight and molasses 4.5 to 100 parts by weight of water 6.5 parts by weight of the mixed microbial spawn is cultured by intermittently stirring and aeration. As shown in Test Example 1 to be described later, when culturing the complex microbial spawn in the component ratio as described above, the microbial population is exploded in a short time. In particular, in order to explode the microbial population, agitation and aeration should be performed at appropriate time intervals. The agitation of the culture solution may be performed by a tool for agitating the culture solution, and the aeration of the culture solution may be performed by an air pump or the like installed inside the culture vessel.

If the agitation time of the culture solution is too long, the microorganisms cannot take sufficient rest and the increase of the number of microorganisms is slow. If the agitation time of the culture solution is too short, the food is concentrated in the culture medium and the increase of the microbial population is slow. If the aeration time of the culture medium is too long, anaerobic microorganisms that do not require oxygen are killed and the increase of microbial population is slow. If the aeration time of the culture medium is too short, the aerobic microorganism that requires oxygen is killed and the increase of microbial population is slow.

In the culturing step 30 according to the present embodiment, in order to explode the microbial population for the same reason as described above, the culturing step is carried out a process of stopping agitation and aeration for about 4 hours after stirring and aeration for about 2 hours. By repeating, intermittent stirring and aeration are performed. The reason for stopping the agitation and aeration is about twice as long as the agitation and aeration time is that the microorganism population must be explosive only if the microorganism has a longer time to rest after metabolism than the time the microorganism is fed. Because it increases.

In the measuring step 40, the pH of the liquid material cultured in the culturing step 30 is measured using a pH meter. The pH of the liquid material may be measured in a state in which the pH meter is immersed in the liquid material cultured in the culturing step 30, or a small amount of the liquid material cultured in the culturing step 30 may be collected and measured by a pH meter. When the pH measurement value is between 3 and 4.5, the cultured liquid material is filtered and used as a soil improver, and the cultured liquid material is characterized by aroma and sour taste. On the other hand, when the pH value of the liquid material cultured in the culture step 30 is not between 3 to 4.5, the cultured liquid material is disposed of.

In the filtration step 50, the soil improver is completed by filtering the liquid substance whose pH value is measured within the predetermined range in the measurement step 40. Herein, the predetermined range is preferably 3 to 4.5. For example, the filtration step 50 may filter the liquid material having a pH value of 3 to 4.5 measured in the measurement step 40 with a filter having a pore size of 200 mesh. A filter having a pore size of 200 mesh may be manufactured by drilling a plurality of 200 mesh size holes in the bottom of a container made of acrylic material. When a liquid substance having a pH value of 3 to 4.5 is introduced into a filter of such a container type, the liquid substance discharged from the filter becomes a soil improver.

In the measurement step 40, the liquid material measured to have a pH value of 3 to 4.5 , Bacillus subtilis , Lactobacillus plantarum , Saccharomyces cerevisiae (Saccharomyces cerevisiae) In addition to the complex spawn, lysine, and propolis , which contains), the molasses and the remaining by-products left by the spawn are included. Molasses in the liquid substance is easily rotting, so if a large amount of molasses is contained in the liquid substance, the liquid substance is deteriorated during the long-term storage of the liquid substance. Similarly, residual by-products in the liquid substance deteriorate the liquid substance during long term storage of the liquid substance.

Therefore, molasses and residual by-products in the liquid substance, measured at pH values of 3 to 4.5, must be removed because they deteriorate the soil improver. The aforementioned complex microbial spawn, lysine and propolis have the property of passing through 200 mesh sized holes, while molasses and residual byproducts do not pass through 200 sized holes. Therefore, molasses and residual by-products in the liquid substance can be removed by filtering the liquid substance whose pH value is measured within 3 to 4.5 by the method described above. As such, since the soil improver that has undergone the filtration step 50 does not contain molasses and residual by-products, deterioration of the soil improver can be prevented and the condition of the soil improver can be preserved for a long time. For example, storage of the soil improver for 6 months was not decayed and the population of beneficial bacteria remained almost unchanged.

On the other hand, the filtration process as described above may be repeated several times to more reliably remove components and by-products unnecessary for the present invention from the liquid substance having a pH value of 3 to 4.5. For example, four filters having a pore size of 200 mesh can be prepared, and a liquid material whose pH value is measured within 3 to 4.5 can be passed through the four filters in turn. That is, the liquid material filtered by any one filter is passed to the next filter to be filtered. The liquid material that passes through the fourth filter is a soil improver and is placed on the market in a container.

Soil modifier according to the present embodiment is a simple process consisting of a water purification step 10, a dosing step 20, a culture step 30, a measurement step 40, a filtration step 50, etc. according to the above-described series of processes It can be produced only. In addition, since any process in the manufacturing process of the soil improver does not require chemicals can provide an environmentally friendly soil improver. On the other hand, the soil improver may use a diluent mixed with a certain amount of water and soil improver, the diluent mixed with a certain amount of water and soil modifier can be sprayed on the soil, or sprayed on plants.

In addition, the soil improver prepared according to the above-mentioned steps is applied to the soil to remove the various pollutants in the soil to clean the soil, and by providing a plant planted in the soil and the beneficial components produced by the activities of various microorganisms Promotes growth.

Hereinafter, the soil improving agent using the complex microbial seed of the present invention will be described in detail through Examples, Comparative Examples, and Test Examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.

<Example 1>

According to the embodiment of the present invention described above was prepared a soil improver as follows. Even if omitted below, the descriptions given above will be followed. 1000 Kg of tap water was put into a heating vessel, and heated to 80 ° C. for 12 hours. Subsequently, while cooling the water slowly, when the temperature of the water reached the 40 ℃ range 800Kg of water was added to the culture vessel. Subsequently, 50Kg of molasses, 7.5Kg of complex microbial seed, 1.5Kg of lysine, and 1.5Kg of propolis were added to the culture vessel. Here, the complex microbial spawn was prepared by mixing 40% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum , and 30% by weight of Saccharomyces cerevisiae. .

Then, 200Kg of water remaining in the culture vessel was added. Subsequently, stirring and aeration were performed for 2 hours on the culture medium in the culture vessel, and the process of stopping the stirring and aeration for 4 hours was repeated for a total of 72 hours, and the temperature of the culture solution was maintained at 40 ° C. Subsequently, the liquid material having a pH value of 4 was filtered four times using a filter having a pore size of 200 mesh, and the liquid material passed through the fourth filter was placed in a storage container to complete the preparation of the soil improver.

<Example 2>

According to the embodiment of the present invention described above was prepared a soil improver as follows. Even if omitted below, the descriptions given above will be followed. 1000 Kg of tap water was put into a heating vessel, and heated to 80 ° C. for 12 hours. Subsequently, while cooling the water slowly, when the temperature of the water reached the 40 ℃ range 800Kg of water was added to the culture vessel. Subsequently, 50Kg of molasses, 7.5Kg of complex microbial seed, 1.5Kg of lysine, and 1.5Kg of propolis were added to the culture vessel. Here, the complex microorganism spawn is 35% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum, 30% by weight of Saccharomyces cerevisiae , and red non-sulfur bacteria 5% by weight was prepared by mixing.

Then, 200Kg of water remaining in the culture vessel was added. Subsequently, stirring and aeration were performed for 2 hours on the culture medium in the culture vessel, and the process of stopping the stirring and aeration for 4 hours was repeated for a total of 72 hours, and the temperature of the culture solution was maintained at 40 ° C. Subsequently, the liquid material having a pH value of 4 was filtered four times using a filter having a pore size of 200 mesh, and the liquid material passed through the fourth filter was placed in a storage container to complete the preparation of the soil improver.

Comparative Example 1

According to the embodiment of the present invention described above was prepared a soil improver as follows. Even if omitted below, the descriptions given above will be followed. 1000 Kg of tap water was put into a heating vessel, and heated to 80 ° C. for 12 hours. Subsequently, while cooling the water slowly, when the temperature of the water reached the 40 ℃ range 800Kg of water was added to the culture vessel. Subsequently, 50Kg of molasses and 7.5Kg of complex microbial seed were added to the culture vessel. Then, 200Kg of water remaining in the culture vessel was added. Here, the complex microbial spawn was prepared by mixing 40% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum , and 30% by weight of Saccharomyces cerevisiae. .

 Subsequently, stirring and aeration were performed for 2 hours on the culture medium in the culture vessel, and the process of stopping the stirring and aeration for 4 hours was repeated for a total of 72 hours, and the temperature of the culture solution was maintained at 40 ° C. Subsequently, the liquid material having a pH value of 4 was filtered four times using a filter having a pore size of 200 mesh, and the liquid material passed through the fourth filter was placed in a storage container to complete the preparation of the soil improver.

Comparative Example 2

According to the embodiment of the present invention described above was prepared a soil improver as follows. Even if omitted below, the descriptions given above will be followed. 1000 Kg of tap water was put into a heating vessel, and heated to 80 ° C. for 12 hours. Subsequently, while cooling the water slowly, when the temperature of the water reached the 40 ℃ range 800Kg of water was added to the culture vessel. Subsequently, 50 Kg of molasses, 7.5 Kg of complex microbial seed, 3.5 Kg of lysine, and 4 Kg of propolis were added to the culture vessel. Then, 200Kg of water remaining in the culture vessel was added. Here, the complex microbial spawn was prepared by mixing 40% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum , and 30% by weight of Saccharomyces cerevisiae. .

 Subsequently, stirring and aeration were performed for 2 hours on the culture medium in the culture vessel, and the process of stopping the stirring and aeration for 4 hours was repeated for a total of 72 hours, and the temperature of the culture solution was maintained at 40 ° C. Subsequently, the liquid material having a pH value of 4 was filtered four times using a filter having a pore size of 200 mesh, and the liquid material passed through the fourth filter was placed in a storage container to complete the preparation of the soil improver.

<Test Example 1>

The results of Table 1 disclosed below are the results of testing at the Chungnam National University Agricultural Science and Technology Center located in Daejeon Metropolitan City, Chungcheongnam-do, Korea to confirm the superiority as a soil improver through a microbial test according to an embodiment of the present invention.

Inspection items	unit	test results
Bacillus subtilis	cfu / g	3.0 × 10 9
Lactobacillus plantarum	cfu / g	2.0 × 10 10
Saccharomyces cerevisiae	cfu / g	7.0 × 10 9

Table 1 is a test report of the first embodiment of the soil improving agent prepared according to the Bacillus subtilis (Bacillus subtilis), Lactobacillus plan tareom (Lactobacillus plantarum), and Saccharomyces busy in serenity (Saccharomyces cerevisiae) in microbial populations.

According to Example 1 of the present invention, when lysine, propolis, and molasses were fed at the same time as the feed of the complex microbial spawn, the microbial spawn was cultured , Bacillus subtilis, Lactobacillus plantarum, and At least 3.0 x 10 ⁹ populations of Saccharomyces cerevisiae microorganisms were detected.

<Test Example 2>

Table 2 is a test report of the comparative example of a soil conditioner prepared according to 1 Bacillus subtilis (Bacillus subtilis), Lactobacillus plan tareom (Lactobacillus plantarum), and Saccharomyces busy in serenity (Saccharomyces cerevisiae) in microbial populations.

Inspection items	unit	test results
Bacillus subtilis	cfu / g	5.0 × 10 6
Lactobacillus plantarum	cfu / g	4.0 × 10 6
Saccharomyces cerevisiae	cfu / g	1.5 × 10 7

In Comparative Example 1, as shown in the related art, molasses was used as a culture feed for microorganisms, and the microorganism spawn was cultured. Compared with Table 1 and Table 2, the population of microorganisms increased significantly when lysine, propolis, and molasses were added together. Therefore, when lysine, propolis, and molasses are simultaneously introduced into the culture feed of the microorganism, it can be seen that it is effective in enhancing the population of the microorganism.

<Test Example 3>

Inspection items	unit	test results
Bacillus subtilis	cfu / g	4.0 × 10 7
Lactobacillus plantarum	cfu / g	6.0 × 10 8
Saccharomyces cerevisiae	cfu / g	4.0 × 10 8

Table 3 is a test report of the Comparative Example 2 of a soil conditioner prepared according to the Bacillus subtilis (Bacillus subtilis), Lactobacillus plan tareom (Lactobacillus plantarum), and Saccharomyces busy in serenity (Saccharomyces cerevisiae) in microbial populations.

In Comparative Example 2, lysine, propolis, and molasses were simultaneously added to feed the complex microbial spawn to cultivate the complex microbial spawn, but the amount of lysine and propolis was added in an amount different from the mixing ratio of the present invention. Incubated. As a result, it can be seen that the population of Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae microorganisms decreased compared with Table 1.

<Test Example 4>

In order to confirm whether the soil improver prepared according to Example 1 and the soil improver prepared according to Example 2 were suitable for crop growth, the following test was conducted using lettuce.

First, for 28 days, seedlings (planting young seedlings used for breeding as cultivated crops or nails) were formally planted (planting seedlings grown on hotbeds or seedlings in fields). In Experimental group 1, the soil modifier prepared according to Example 1 was sprayed to the soil by diluting 1000 times with water, and in Experimental group 2, the soil modifier prepared according to Example 2 was diluted to 1000 times and sprayed onto the soil. There was no separate treatment in the control group for comparison. The herb length (the length from the index of the herbaceous plant to the tip of the herbaceous plant) and the number of leaves (number of plant leaves) of each group were shown in Table 4, and lettuces with similar growth rates were used for the test.

	Control	Experiment group 1	Experiment group 2
Extra long (cm)	8.25	8.42	8.48
Coniferous tree	9.5	9.8	9.8

Thereafter, in Experimental Group 1, the soil modifier prepared according to Example 1 was sprayed on the soil by diluting it 1000 times with water, and in Experimental Group 2, the soil modifier prepared according to Example 2 was diluted and sprayed by 1000 times. Each group was sprayed with soil modifiers every five days, and the rest of the cultivation was in compliance with the Rural Standard Farming Manual.

After 10 days from the date of establishment, the growth state of the lettuce was first examined, and the soil improver was sprayed twice in Experiment Group 1 and Experiment Group 2 for 10 days, and the results are shown in Table 5 below. As shown in Table 5, it can be seen that the growth efficiency of lettuce was higher in Experimental Group 1 and Experimental Group 2 to which the soil improvers prepared in Examples 1 and 2 were applied than the control group.

Primary investigation (10 days after formality)

	Control	Experiment group 1	Experiment group 2
Extra long (cm)	12.11	12.88	13.44
Coniferous tree	9.5	10.7	11

In the case of the number of leaves, Experimental Group 1 was about 1 sheet long, and Experimental Group 2 was about 1.5 sheets more than the control group. In the case of ultra long, the control group was about 3.86 cm long, while the experimental group 1 was about 4.46 cm long, and the experimental group 2 was about 4.96 cm long. As a result of the first investigation, the number of leaves was larger in the experimental group 1 and the experimental group 2 than the control group, and the height was longer.

Next, Table 6 below shows the growth state of lettuce after 20 days from the date of formulation, and the soil improver was sprayed four times in Experimental Group 1 and Experimental Group 2 for 20 days.

Second investigation (20 days after formality, 10 days after primary investigation)

	Control	Experiment group 1	Experiment group 2
Extra long (cm)	16.25	19.08	21.3
Coniferous tree	12.2	15.8	17.0

In the case of the number of leaves, Experimental Group 1 was about 3 in number and Experimental Group 2 was about 5 in comparison with the control group. In the case of ultra long, the control group was about 4.14 cm long, while Experiment 1 was about 6.2 cm long, and Experiment 2 was 7.86 cm long. As a result of the second investigation, the number of leaves was more than three in the experimental group compared with the control group, and in the case of super long, the experimental group was significantly longer than the control group.

Through Tables 5 and 6, it can be seen that the growth and development of lettuce grown in the soil sprayed with the soil improver prepared according to Examples 1 and 2 was better. Therefore, it was confirmed that the soil improving agents prepared according to Examples 1 and 2 of the present invention promote the growth of plants by improving the soil.

In addition, the growth efficiency of Experiment Group 2 using the soil modifier prepared according to Example 2 containing photosynthetic bacteria was higher than that of Experiment Group 1 using the soil modifier prepared according to Example 1 not containing photosynthetic bacteria. Therefore, the addition of photosynthetic bacteria is thought to increase the efficacy of the soil improver.

Therefore, to prepare a soil improver with a mixing ratio of 0.7 to 0.9 parts by weight of the composite microorganism spawn 0.7 to 0.9 parts by weight, lysine 0.1 to 0.3 parts by weight, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses according to the present invention. In the same time, it is possible to prepare a soil improver with a large increase in the number of microorganisms.

In view of all the results of the Examples, Comparative Examples, and Test Examples of the present invention, when lysine, propolis, and molasses are simultaneously used as the culture feed of the complex microbial spawn, it is effective in increasing the population of the complex microbial spawn, According to the mixing ratio suggested, it is estimated that the effect of increasing the population is increased.

So far, the present invention has been described with reference to preferred examples, comparative examples, and test examples. Although the present invention has been described in detail through Examples, Comparative Examples, and Test Examples, it is not intended to limit the present invention but merely to illustrate the present invention. Therefore, it will be apparent to those skilled in the art that the present invention is not limited by these examples in accordance with the gist of the present invention. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Claims (10)

1. In the manufacturing method of the soil improving agent using the complex microbial spawn,

   Bacillus subtilis (Bacillus subtilis), Lactobacillus plan tareom (Lactobacillus plantarum), and the MRS celebrity busy in Saccharomyces lysine with (Saccharomyces cerevisiae) is a compound seed a number of micro-organisms containing, propolis, molasses, and water Injecting step into the culture vessel;

   A culturing step of culturing the complex microbial spawn added to the culture vessel using a culture solution formed by mixing lysine, propolis, molasses and water added to the culture vessel;

   A measuring step of measuring a pH value of the liquid substance cultured in the culturing step; And

   And a filtration step of completing the soil improving agent by filtration of the liquid substance whose pH value is measured within a predetermined range in the measuring step.
2. The method of claim 1,

   The complex microorganism spawn is Lactobacillus casei , Lactobacillus acidophilus , Lactobacillus leuconostoc , Lactobacillus brevis , Lactobacillus brevis , Streptococcus faecalis faecalis , Bacillus putrificus , Bacillus cereus , Pseudomonas fluorescens , and Aspergillus oryzae The manufacturing method of the soil improving agent manufactured using the complex microorganism spawn.
3. The method of claim 1,

   The composite microbial spawn further comprises at least one of green sulfur bacteria, red sulfur bacteria, and red non-sulfur bacteria.
4. The method of claim 1,

   In the input step, 0.7 to 0.9 parts by weight of the complex microorganism spawn, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses,

   The culturing step is 0.7 to 0.9 weight based on 100 parts by weight of the water using a culture solution mixed in a composition ratio of 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses based on 100 parts by weight of water. A method for producing a soil improving agent produced using a complex microbial spawn, characterized in that the complex microbial spawn is cultured.
5. The method of claim 4, wherein

   The culturing step may be performed by aeration while simultaneously stirring intermittently with 0.7 to 0.9 parts by weight of the complex microorganism spawn, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses. A method for producing a soil improving agent produced using a complex microbial spawn, characterized in that the complex microbial spawn is cultured.
6. The method of claim 5,

   The culturing step is characterized in that the intermittent agitation and aeration by repeating the step of stopping the agitation and aeration for a second time longer than the first time after performing the stirring and aeration for a first time Method for producing a soil improving agent prepared using a complex microbial spawn.
7. The method of claim 5,

   The culturing step is a method for producing a soil improving agent prepared using a complex microbial spawn characterized in that the intermittent stirring and aeration while maintaining the temperature of the culture medium in the range of 35 ~ 45 ℃.
8. The method of claim 1,

   The filtration step is a method for producing a soil improver using a complex microbial spawn, characterized in that to complete the soil improver by filtering the liquid material measured in the pH value range of 3 to 4.5 in the measuring step.
9. The method of claim 1,

   In the filtration step, the liquid material corresponding to the soil improver is discharged from the filter by filtering the liquid material whose pH value is measured within a predetermined range in the measuring step by using a filter having a pore size of 200 mesh. A method for producing a soil improving agent produced using a complex microbial seed.
10. A soil improving agent prepared by the method of claim 1.

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